ANATOMY LEARNING RESOURCE CENTER

Transcription

1 College of Medicine

2 Central Laboratories Catalog ANATOMY LEARNING RESOURCE CENTER Lab Name Location Equipment/ Contents Functions Anatomy Museum M Anatomy Lab A M Anatomy Lab B M Plastinated Specimens (Full Parts and Cross Sections) Setting for Small Group Studying Cupboards for Plastic & Plastinated Parts Data Show Computers Histology Lab A M Light and Binocular Microscopes for Life Histology Lab B M Demonstration Computers Demonstration/ Dissection Room Cadaver Store Room Store Room M M27-051C M27-053A Setting for Dissecting Human Cadavers Life Demonstrating Video System Cadavers & Parts are Preserved in Tanks Museum for Demonstrating and Teaching Anatomy Students are Allowed to Self-Study Small Group, Interactive Teaching Anatomy Office for the Lab Technician Small Group, Interactive Teaching Anatomy Virtual Reality 3D Cyber Anatomy System Small Group, Interactive Teaching Histology For Running Practical Examinations (OSPE) For PBL Tutorial Sessions Dissection of Human Cadavers For Life Demonstration of Human Cadavers and Self-Study Preserving Cadavers Storing Chemicals, Dissection Instruments, Plastic Models, and Anatomy Charts

3 The Learning Resource Center of Anatomy is available in M27 for learning and teaching human Anatomy for the medical students. It is formed of eight laboratories. Students gain the practical skills required for the 3D understanding of the gross anatomy and the microscopic structures of the human body. These labs are as follows: 4 Gross Anatomy Labs. (One for Upper and Lower Extremities, One for Head & Neck, One for Abdomen and Pelvis) 2 Histology Labs One Anatomy Museum One Demonstration Room PURPOSE The purpose of the Anatomy Learning Resource Center is to provide medical learners the optimal tools that enable them to transform their knowledge in Anatomy into three dimensional concepts for the betterment understanding of the human body. We seek motivating learners and integrating knowledge with the applied and clinical aspects of anatomy. The Anatomy labs are designed to help learners for the interactive and self-directed learning and to encourage them for peer presentation guided by the class objectives given to them a head, e.g. fit for purpose. EQUIPMENT AND INSTRUMENTS Every Lab is Equipped with Computers, Data Show and Accessibility to Internet More than 70 Plastinated Parts Covering all Parts of the Human Body Plastic Models and Skeletons of the Highest Quality Covering all Body Parts and Organs Five Formalinized Bodies used for Dissection and Demonstration Video Camera with Plasma Screen for Live Demonstration for a more than 50 Students Virtual Reality 3D Cyber Anatomy System Binocular Microscopes for Histological Examination of Slides Full Sets of Histology Slides for all the Body Plastinated Models in Anatomy Learning Resource Tissues More than 20 Illustrative Anatomy Charts of the Different Body Systems 271

4 Central Laboratories Catalog Full Set of X-Ray films (Including Digital) for Studying the Normal Radiological Anatomy ANATOMY TEACHING Human anatomy is taught in five main themes: Regional Anatomy Functional Anatomy Radiological Anatomy Surface Anatomy Clinical Anatomy

5 HUMAN PHYSIOLOGY LABORATORY One of the major academic challenge a student faces during the preclinical years is to understand the core principles of physiology and their application. Confucius once said, I hear and I forget, I see and I remember, I do and I understand. We also firmly believe that all basic concepts in physiology need to be translated into applied knowledge by active learning. Such an approach help students in assimilating the theoretical knowledge. With this principle in mind, a physiology lab in the Basic Medical Science Department was established. EQUIPMENT AND INSTRUMENTS Biopac Data Acquisition Unit Stethoscope Amplifier Electrode Lead Set Disposable Electrodes Electrode Gel Push-Button Hand Switch Computer System with Analysis Software Headphones Electromyography Phonocardiography Reaction Time 273

6 Central Laboratories Catalog MEDICAL BIOCHEMISTRY LABORATORY (UNDER CONSTRUCTION) The mission of this Laboratory is to provide the medical student with an insight to understand how the blood tests are conducted as well as recognizing the major limitations and obstacles. The goal is to obtain basic laboratory skills to obtain reproducible data from biochemical experiments as well as to analyze and interpret the results of their laboratory experiments in a team work of problem-based assignments. EQUIPMENT AND INSTRUMENTS Spectrophotometers ELISA Reader Centrifuges Micropipettes Lab Introduction, Basics and Safety Glucose Estimation and Basic Spectrometry DNA Extraction Calcium Estimation Hemoglobin Electrophoresis Cholesterol Estimation Introduction to Kinetic Studies and ALT Estimation

7 HUMAN BIOLOGY LABORATORY This Human Biology Laboratory is specifically for Medical and Dental students in Foundation Year. The laboratory session is divided into two sections. Human Biology 1 (HB1) and Human Biology 2 (HB2). HB1 is offered in Fall and Spring semesters while HB2 in Spring and Summer semesters. Students are exposed to different laboratory activities related to body tissues, blood components and Cardiovascular system learned during their lectures. EQUIPMENT AND INSTRUMENTS Human Biology 1 Compound Microscope Prepared Tissue Slides Compact Bone Model Human Biology 2 Compound Microscope Prepared Peripheral Blood Slides Micro Pipette Hemocytometer Hematocrit Centrifuge Machine Westergren ESR Tube Stethoscope BP Measurement Set ECG Machine Human Biology 1 Introduction to Laboratory Safety Compound Microscope and its uses Wet Mount Preparation using Cheek Cells 275

8 Central Laboratories Catalog Determination of Cell Diameter Identification of Different Human Body Tissues Under the Microscope Human Biology 2 Introduction to Laboratory Safety Introduction to Basic Laboratory Instruments Osmosis & Tonicity Learning How to use Micro Pipette WBCs and RBCs Morphology Manual Determination of: o Total White Blood Cell Count using Hemocytometer o Differential White Blood Cell Count o Hematocrit and Hemoglobin Values Erythrocyte Sedimentation Rate (ESR) Heart Sounds and Peripheral Pulses Blood Pressure ECG

9 IMMUNOLOGY LABORATORY The main theme of this Laboratory is to introduce various experiments and techniques in the field of immunology. Our basic techniques include cell isolation and cell culturing. Regarding cell culture, this lab aims at teaching the students how to perform cell culture in sterile conditions, in addition to the preparation of different culture media and buffers. Furthermore, it is quite crucial to highlight the laboratory safety and equipment along the experimental approaches. EQUIPMENT AND INSTRUMENTS Centrifuges Micro-Pipettes Water Baths Culture Flasks and Falcon Tubes PH Meters Balances CO2 Incubators Hemocytometers Inverted Light Microscope Biological Safety Hood Isolation of Human and Mouse Lymphocytes such as Natural Killer (NK) Cells, Monocytes and Dendritic Cells Culture of Primary Isolated Cells as Well Immortalized Cell Lines Determination of Cell Viability Preparation of Various Culture Media and Buffers Study the Effect of Different Treatments on NK Cell Killing of Target Cells such as Cancer Cells Study the Effect of the Various Treatments on NK Cell Migration Toward Target Cells Study the Expression of Cell Surface Receptors using Flow Cytometry 277

10 Central Laboratories Catalog CHEMOKINE AND CYTOKINE LABORATORY In this Laboratory, students are introduced to the effects of chemokines and cytokines on various cell types. The experiments are accomplished using several techniques. EQUIPMENT & MATERIAL Centrifuges Micro-Pipettes Whatman Nucleopore Membranes Giemsa Stain Modified Boyden Chambers Inverted Light Microscope Chemical Reagents and Kits Conical Tubes ELX808 Microplate Reader FLX800 Plate Reader Bioplex-200 Multiplex System Investigate the Migration of Lymphocytes such as Natural Killer Cells Towards their Respective Targeted Cells as well as Various Chemokines using Modified Boyden Chambers and Nucleopore Membranes Analyze the Expression as well as Quantity of Cytokines and Chemokines using ELISA and Bioplex-200 Systems Examine the Expression of Chemokine Receptors on the Surface of NK and other Cell Types Evaluate the Concentrations of Various Chemokines in Samples

11 TISSUE BANK LABORATORY The main aim of this Laboratory is to familiarize the students with tissue-based research techniques and increase their understanding about human diseases, specifically on the genomic and proteomic level. Furthermore, tissue banks make it possible and feasible to utilize human tissue in research. EQUIPMENT & MATERIAL Centrifuges -80 C Freezers Liquid Nitrogen Tanks Biological Safety Hood CO2 Incubators Various Fluorescence and Optical Microscopes HM-NX Cryostat Chemical Reagents Tissue Printer Tissue Processor Tissue Sonicators Tissue Homogenizers Preparation of Freshly Obtained Cells and Tissues for Freezing Processing the Samples via Paraffin Embedding, Cutting and Staining with Hematoxylin and Eosin Protein Detection using Fluorescence After Sectioning of the Embedded Tissue Immunohistochemistry Protocols for Imaging of Internal Cells and Tissue Structures Printing Tissues such as Skin and Ears, Among any others Examination of Cells under Fluorescence Microscopes 279

12 Central Laboratories Catalog NK CELL RESEARCH LABORATORY In this Laboratory, we focus on investigating natural killer cell viability, cytotoxicity and migration. NK cells can perform several important functions such as immune-regulation via secretion of cytokines and chemokines, and hence interact with dendritic cells and the adaptive immune system. Additionally, they are capable of lysing and destroying stressed cells such virally infected and tumor cells, via release of cytotoxic granules. EQUIPMENT & MATERIAL Centrifuges Pipettes Microscope Chemical Reagents and Kits Culture Flasks and Tubes Fluorescent Labelled Antibodies for FACS CO2 Incubators FLX800 Plate Reader LS55 Fluorescence Spectrometer NK Cell Isolation and Culture Cytotoxicity Assay using Calcein-AM and FLX800 Plate Reader Flow Cytometric Analysis of Diverse Extracellular and Intracellular Markers Identification of NK Cells Subtypes Calcium Mobilization Test to Measure Ca2+ Fluxes After Different Treatments

13 IN-VITRO CANCER RESEARCH LABORATORY This Laboratory introduces students to various techniques and procedures used in cancer cell handling. Different cell lines for various experiments and projects are cultured with great care in order to maintain their safe handling under sterile conditions. EQUIPMENT & MATERIAL CO2 Incubators Autoclaves Culture Flasks and Tubes Pipettes Microscope Proper Preparation of Condition Media According to the Requirements of each Cell Line Perform Cell Washing, and Calculate Seeding Density and Volumes Thawing and Cryopreservation of the Cells Test for Cell Viability and Perform Cell Counting 281

14 Central Laboratories Catalog HISTOPATHOLOGICAL LABORATORY The main aim of this Laboratory is to provide the users with various techniques and procedures used in histopathology in order to aid in the diagnosis of human diseases. These methods include tissue processing, paraffin embedding, tissue sectioning, slide staining and microscopic examination. EQUIPMENT & MATERIAL -80 C Freezers EXCELSIOR AS Tissue Processor HM 355 Microtome Pipettes Fluorescence Microscope and Optical Microscope HM NX Cryostat Chemical Reagents Preparation of Tissues by Paraffin Embedding, Tissue Cutting and Staining with Hematoxylin and Eosin. The Standard Procedure Consists of Tissue Fixation, Dehydration, Rehydration, and Subsequent Embedment of Tissue into Paraffin Perform Immunohistochemistry for Detection of Specific Markers in Human Tissues using Fluorescent Labelled Antibodies

15 ASTHMA RESEARCH LABORATORY The Asthma Research Laboratory focuses on investigating the mechanisms that drive the pathogenesis of respiratory diseases, asthma in particular, and in identifying clinical and diagnostic biomarkers across varying severities of asthma. This Laboratory introduces students to basic molecular biology techniques and reagent preparations. Furthermore, the students develop hands on knowledge on various molecular and cellular functional assays that provide them a better understanding of the events that occur within the cell. EQUIPMENT & MATERIAL Biological Safety Cabinets Optical Microscope Centrifuges Incubators and Water Baths PH Meters Spectrophotometers Bioplex-200 Multiplex System Confocal Microscopy Real-time PCR Machines NGS DNA Sequencer Culturing, Counting and Freezing of Cell Lines Investigation of Protein Expression in Cells using Western Blots and Flow Cytometry Examination of Protein Co-Localization using Fluorescence/ Confocal Microscopy Quantification of Gene Expression using Quantitative Real-Time Polymerase Chain Reaction (qrt-pcr) Preparation of Histological Sections and Analysis using Immunohistochemistry DNA, RNA and Protein Extraction from Cell Lines and Biopsies DNA and RNA Purification Molecular Cloning Transformation of Competent Cells 283