ENZYME TECHNOLOGY TO DRIVE PROFITS IN BIOGAS PRODUCTION Sandra Hinz, PhD, DuPont Industrial Biosciences 5 July 2017

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1 ENZYME TECHNOLOGY TO DRIVE PROFITS IN BIOGAS PRODUCTION Sandra Hinz, PhD, DuPont Industrial Biosciences 5 July 2017

2 TWO EARLY PIONEERS IN MODERN INDUSTRIAL BIOTECHNOLOGY Genencor Genentech & Corning form Genencor New joint owners Kodak & Cultor form Genencor Int l Genencor acquires: Gistbrocades,Solvay,Wuxi, Enzyme Bio Systems, Rhodia Enzymes Danisco acquires Genencor Construction of Nevada CE facility completed DuPont Genencor initate PDO collaboration 1980 s DuPont DDCE joint venture formed DuPont s investments in biotechnology begin in earnest DuPont Merck JV formed 1995 DuPont Tate & Lyle JV established DOE funds DuPont Integrated Corn Biorefinery Program 2008 First sales of biobased Sorona polymer Butamax JV established for biobutanol DuPont acquires Danisco IB business formed First shipment of new cold-water protease for laundry DuPont acquires Dyadic industrial biotech business DuPont Copyright 2017 DuPont. All rights reserved. 2

3 INDUSTRIAL BIOTECHNOLOGY HOLDS THE POTENTIAL TO ADD AND CAPTURE VALUE ACROSS DIVERSE INDUSTRY SEGMENTS VALUE DRIVERS PRODUCT PERFORMANCE PROCESS EFFICIENCY & QUALITY ALTERNATIVE, SAFER AND CLEANER PROCESSES GROWTH OPPORTUNITIES ACROSS DIVERSE INDUSTRIES Novel functionality Higher performance Sustainable or natural ingredients Higher yields Lower costs Less waste Alternatives to harsh chemicals Alternatives to petroleum Water and energy conservation Animal Nutrition Home Care & Cleaning BIOTECH SOLUTIONS BIOBASED INGREDIENTS BIOCATALYSTS TECHNOLOGY AND PROCESSING AIDS Grain Processing Bioenergy Apparel Proteins including enzymes Biopolymers Probiotics Amino acids Enzymes Yeast Process technology Enzymes Ethanologens Food & Beverage Carpet Personal Care CASE STUDY: FOOD ENZYMES + CASE STUDY: FUEL ETHANOL + CASE STUDY: TEXTILES + Copyright 2017 DuPont. All rights reserved. 5 July

4 WORLDWIDE AND WORLD CLASS PEOPLE, SCALE AND CAPABILITIES ~ 2500 dedicated colleagues worldwide 6 global R&D sites 8 applications labs 17 operations sites Copyright 2017 DuPont. All rights reserved. 4

5 COLLABORATION PROJECT: DEMETER EU funded Project Demonstrating more efficient enzyme production to increase biogas yields 1. to improve and scale-up the enzyme-producing fermentation process to yield at least 20% more protein while retaining quality 2. to improve downstream processing of the fermentation broth leading to a cost reduction of at least 15% 3. to demonstrate the improved production process in a L pilot plant 4. to demonstrate the industrial and economic feasibility to apply a cost-effective enzyme product in biogas plants 5. to develop a predictive model of the effect of enzyme addition on the biogas yield of a given fermentation process 6. to analyze the impact of the developed processes and products on environment and economy Copyright 2017 DuPont. All rights reserved. 5 July

6 BOTTLENECKS IN BIOGAS PRODUCTION High viscosity in the reactor reduces mixing and conversion Long retention time in reactor (up to 150 days) means a high loading of substrate is required to achieve high gas production Incomplete conversion results in a large digestate waste stream Low-cost lignocellulosic substrates show relatively poor biogas yield Copyright 2017 DuPont. All rights reserved. 5 July

7 ENZYME TECHNOLOGY FOR IMPROVED BIOGAS PRODUCTION Enzyme addition aids break down complex polymeric substrates into smaller oligomers that are more suitable for biogas-producing organisms: Increasing the fermentable fraction of the substrate (more gas, less digestate) Improvement of degradation kinetics (higher feeding rates) Improvement of rheological characteristics of reactor (viscosity reduction) Easy enzyme introduction to biogas processes Can be added into primary digester or as a pretreatment step Copyright 2017 DuPont. All rights reserved. 5 July

8 ENZYME TECHNOLOGY FOR IMPROVED BIOGAS PRODUCTION Make complex feedstocks and substrates simpler Classic model of converting insoluble cellulose into soluble gluco-oligomers and glucose Endo-cellulase* Exo-cellulase β-glucosidase Image from U.S. Department of Energy Copyright 2017 DuPont. All rights reserved. 5 July

9 ENZYMES TO MATCH WITH DIFFERENT BIOGAS SECTORS Cellulase / Hemicellulase OPTIMASH AD-100 General Liquefaction Agricultural waste Protease Cow, swine, poultry manure α-amylase and glucoamylase Wastewater Pectinase Mannanase Food Waste MSW (Municipal Solid Waste) Lipase FOG (Fats, Oils, Grease) Copyright 2017 DuPont. All rights reserved. 5 July

10 ENZYMES CAN REDUCE FEEDSTOCK REQUIRED Agricultural co-digestion plant trial: 1 MW biogas plant Cow slurry, corn silage and triticale; CSTR (Continuously Stirred Tank Reactor) operating at 41 C 12% decrease in feedstock required to maintain biogas output (~12% increase in biogas yield) Copyright 2017 DuPont. All rights reserved. 5 July

11 ENZYMES CAN REDUCE SUBSTRATE VISCOSITY Agricultural co-digestion plant trial: 1.2 MW biogas plant Chicken muck, whey permeate, beet, corn silage Plug-flow reactor operating at 39.5 C; 35 day retention time Decrease in substrate viscosity by 2-3x Copyright 2017 DuPont. All rights reserved. 5 July

12 LOOKING FORWARD Learning from nature and practical application Importance of understanding which enzyme classes are crucial to the digestion of different feedstocks used in the biogas industry Of these, identify enzymes that are highly active under typical AD conditions Learn how best to apply these enzymes in different biogas plants We will continue to develop new functionalities to improve biogas processes Copyright 2017 DuPont. All rights reserved. 5 July

13 BOCK UK Distributor for DuPont biogas enzymes in UK Supply of sloping walled silage clamps and high quality covers to the anaerobic digestion industry Press release: Contact: Francis Auchincloss Booth L401 Copyright 2017 DuPont. All rights reserved. 5 July

14 Sandra Hinz, PhD DuPont Industrial Biosciences Booth E215 Copyright 2017 DuPont. All rights reserved. 5 July

15 CONNECT WITH US Thank you for your attention DuPont Copyright 2017 DuPont. All rights reserved. 15

16 Copyright 2017 DuPont. All rights reserved. The DuPont Oval Logo, DuPont, The Leaf Globe, Genencor, OPTIMASH and all products denoted with or are registered trademarks or trademarks of E. I. du Pont de Nemours and Company or its affiliates. Nothing contained herein shall be construed as a representation that any recommendations, use or resale of the product or process described herein is permitted and complies with the rules or regulations of any countries, regions, localities, etc., or does not infringe upon patents or other intellectual property rights of third parties. The information provided herein is based on data DuPont believes to be reliable, to the best of its knowledge and is provided at the request of and without charge to our customers. Accordingly, DuPont does not guarantee or warrant such information and assumes no liability for its use. If this product literature is translated, the original English version will control and DuPont hereby disclaims responsibility for any errors caused by translation. This document is subject to change without further notice. 16