Bioanalyzer and Tapestation Summit

Transcription

1 Bioanalyzer and Tapestation Summit March 31, 2016 Santa Clara, CA 1

2 Agenda o 10:00-12:00 o 12:00-2:00 Welcome Overview of Electrophoresis Products- Bioanalyzer & TapeStation Comparison of Systems Sample QC in Multiple Workflows Bioanalyzer Tips & Tricks Bioanalyzer Troubleshooting Tapestation Tips & Tricks Rotations Between: Lunch Data Analysis Workshop Tapestation Demo Bioanalyzer Demo 2 2

3 Bioanalyzer & TapeStation Portfolio 3

4 Microfluidics Product Portfolio 2200 TapeStation System - ScreenTape Technology fully scalable 96-well plate compatible ready-to-use 2100 Bioanalyzer System Electrophoresis in microchannels separation according to mobility (size) cell counting (pressure driven) 4200 TapeStation System ScreenTape Technology Introducing the new TapeStation system Unattended walk away operation with fully automated sample processing for up to 96 samples. 4

5 AGILENT 2100 BIOANALYZER SYSTEM 5

6 The Agilent 2100 Bioanalyzer System First commercially available Lab-on-a-Chip product Introduced 1999 Analysis of biomolecules: DNA, RNA, Proteins and Cells Established standard for the analysis of RNA Standard for sample QC in Next-Generation Sequencing workflows > 33,000 citations Page 6 6

7 2100 Bioanalyzer System One platform Endless possibilities Analytical Platform with exchangable cartridge design - four application areas by easy switch 7

8 Bioanalyzer Kit Portfolio Electrophoretic Separations DNA1000 DNA7500 DNA12000 DNA Assays: Sizing Quantitation PCR products, digests, larger DNA fragments samples in min. Flow Cytometry High Sensitivity DNA 6000 Nano 6000 Pico Small RNA P230 P80 HSP 250 RNA Assays: Quantitation (Sizing in Small RNA) Total RNA, mrna Purity & integrity determination samples in 30 min. RNA Integrity number (RIN) Protein Assays: Sizing Quantitation Cell lysates, column fractions, purified proteins, antibodies etc. 10 samples in min. Flow Cytometry Cell Assays: Analysis of 6 samples in 30 minutes Two color detection Analysis of protein expression in cells 8

9 AGILENT TAPESTATION SYSTEMS 2200 TapeStation System AND 4200 TapeStation System 9

10 Agilent TapeStation Systems Ease of Use: 1 Place ScreenTape and tips in the TapeStation ScreenTape TapeStation 2 Place prepared samples in the TapeStation and press Start on the instrument controller software 3 View analyzed results in around 1 min per sample TapeStation Software 10

11 What technology did Agilent acquire? The TapeStation system and...screentape assays Automated gel electrophoresis system with flexible throughput and 96 well plate compatibility. DNA, RNA and Protein analysis. 11

12 Introducing the 4200 TapeStation System Size: W45 x H50 x D60cm Weight: 25kg Complete System including Laptop 4200 TapeStation Software (no license - free upgrades on webpage) Vortexer with adaptor Plastic consumable start up kit ScreenTape Assay Quick Guides TapeStation Test Tape I&F service bundle Made in Germany 12

13 ScreenTape Assay Portfolio TODAY DNA 4200/2200 TapeStation 2200 ONLY RNA Protein D to 1000bp 1µl of sample HS D to 1000bp 2µl of sample D to 5000bp 1µl of sample HS D to 5000bp 2µl of sample Genomic 200 to DNA >60kbp 1µl of sample RNA 100 to 6000b 1µl of sample HS RNA 100 to 6000b 2µl of sample P to 200kDa 2µl of sample ng/µl LOD 0.1 ng/ul pg/µl LOD 5 pg/ul ng/µl LOD 0.1 ng/ul pg/µl LOD 5 pg/ul ng/µl LOD 0.5 ng/ul ng/µl LOD 5 ng/µl ,000pg/µl LOD 100 pg/µl ng/µl IgG LOD 5 ng/ul DNA Integrit y Numbe r (DIN) *2200 TapeStation obsolescence by end of 2016* RNA Integrity Number equivalent (RIN e ) 13

14 A new standard for genomic DNA QC - the DNA Integrity Number (DIN) DIN functionality for the Genomic DNA ScreenTape assay. Automated numerical software assessment of sample integrity. Independent of sample concentration and analyst. Full range of eukaryotic samples from degraded to intact gdna. Benefits: Easy interpretation of results. Ensures repeatability of experiments. Enables comparison of samples by defining a DIN value or threshold for specific type of samples or preparation 14

15 Ease of use Agilent PLuF NGS QC System offering 4200 TapeStation system 2200 TapeStation system 96 sample walk away system 2100 Bioanalyzer system Established Standard for NGS sample and library QC Ready-to-use ScreenTape Technology Scalable throughput 2-96 samples Genomic DNA analysis DNA Integrity Number (DIN) ScreenTape devices need to be manually exchanged for 96 well plate run (hands on every minutes) Ready-to-use ScreenTape Technology Scalable throughput 1-96 samples Genomic DNA analysis DNA Integrity Number (DIN) Increased product portfolio with D5000 / HS D5000 Automation & Throughput 15

16 Hardware 16

17 Hardware 2100 Bioanalyzer System HW Product Structure 2100 Electrophoresis Bioanalyzer Instrument (G2939AA) no PC reduced I&F service 2100 Bioanalyzer bundles with desktop PC (G2940CA) or laptop (G2943CA) systems require cartridge for electrophoresis (G2947CA) or for flow cytometry (G2948CA) full I&F service 4200 TapeStation System HW Product Structure 4200 TapeStation System (G2991AA) DNA and RNA analysis. System includes laptop, software, accessories, initial set of consumables. 17

18 Software Both software offer great functionality. 18

19 PC and software 2100 Bioanalyzer System 2100 Expert Software Revision B SR3 Instrument control and data analysis Security Pack / 21CFR11 compliance (G2949CA) 4200 TapeStation System TapeStation Software Revision A Separate instrument control and data analysis software packages 21 CFR Part 11 compliance not available Available for download from

20 Software overview 20

21 TapeStation Software - Overview TapeStation Software CONTROLLER TapeStation Software ANALYSIS 21

22 Agilent 4200 TapeStation Controller Software Easy and automated Easy population of sample descriptions Sample names can be entered by copy/paste or imported from a previous file Required Consumables Software calculates the required consumables based on number of samples for analysis Automatic Assay Selection Barcode recognises assay type and available number of ScreenTape lanes for analysis Intuitive sample selection Full flexibility for sample selection independent on sample location. System status & Observations Status, warnings and errors are displayed in the task bar 22

23 Agilent TapeStation Analysis Software Intuitive and familiar Analysis Functions Functions are navigated through a context sensitive ribbon. Gel and Electropherogram View Results can be viewed in familiar gel view or Electropherogram view. Navigation pane Navigate through experiments and individual lanes. Lanes can be selected in any order from the Plate Selector. Result Presentation Customisable views of gel image, eletropherogram and data table. 23

24 Assays 24

25 Current Assay Portfolio Genomic DNA D1000 HS D1000 D5000 HS D5000 DNA assays DNA1000 DNA7500 DNA12000 High Sensitivity DNA RNA HS RNA RNA assays 6000 Nano 6000 Pico Small RNA Protein assays P200 P230 P80 HSP 250 Flow Cytometry Assay Flow Cytometry 25

26 Workflow Overview 2100 Bioanalyzer System Prepare gel-dye mix Prime chip Load chip Select assay RUN 1 = samples in minutes Time (minutes) TapeStation System DNA Prepare samples Buffer Load tips and samples RUN 1 = 1-16 samples in minutes 1 = 96 samples in < minutes 26

27 RNA Assay 27

28 RNA Profile Comparison o Close correlation between 2100 Bioanalyzer and 2200 TapeStation system Agilent 2100 Bioanalyzer instrument RNA 6000 Nano Assay Agilent 2200 TapeStation System RNA ScreenTape Assay 28

29 Resolution alignment o Figures below show how much closer the RNA ScreenTape assay is to the resolution of the RNA assays on the 2100 Bioanalyzer A1 B1 Example Gel Images from the Bioanalyzer (BioA) and TapeStation (TS) Gel image and profile overlay of Eukaryotic RNA (A1 and Blue) and Eukaryotic/Prokaryotic RNA mixture (B1 and Red) 29 29

30 RIN vs. RINe correlation o o o Similar to Eukaryote, Prokaryote samples subjected to heat degradation and dilution across concentration range >200 individual RNA data points (not shown) Median error < ±0.4 RIN units Switching analysis mode is easy! o Can now be done in the analysis software button shown below: NOTE: Depending on the RNA tissue type, profiles may vary and RINe value might be slightly different from RIN 30

31 DNA Assay 31

32 DNA Profile Comparison Bioanalyzer HSDNA assay DNA fragment Tapestation HSD1000 assay DNA fragment 32

33 DNA Profile Comparison Bioanalyzer SSEL Sheared DNA HSDNA assay Tapestation SSEL Sheared DNA - HSD1000 assay SSEL Precapture library - HSDNA assay SSEL Precapture library - HSD1000 assay 33

34 Want to know more? Visit the Bioanalyzer & TapeStation website for most recent information on products, consumables and assays

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36 Agilent 2100 Bioanalyzer Support Tool Box

37 2100 Bioanalyzer Buddy App for ios and Android smartphones Tips & Tricks! Alerts for Expirations - Chips, Reagents, Gels Stopwatches Priming Complete Alert Game for lab downtime! Alerts for Maintenance - Run Complete Alert Compete with friends. Can you Priming Stations & Cartridges move from Dishwasher to Download from itunes and Google Nobel winner? Play 37

38 Questions 38