Capabilities & Services

Transcription

1 Capabilities & Services Accelerating Research & Development

2 Table of Contents Introduction to DHMRI 3 Services and Capabilites: Genomics 4 Proteomics & Protein Characterization 5 Metabolomics 6 In Vitro Sciences 7 Light Microscopy 8 Bioinformatics 9 Contact Us 11

3 INTRODUCTION TO DHMRI The David H. Murdock Research Institute (DHMRI) is a nonprofit life sciences research institute that provides collaborators groundbreaking research and development solutions at the intersection of human health, agriculture and nutrition. Located at the heart of the North Carolina Research Campus (NCRC), DHMRI contains instrumentation, resident expertise, and well-equipped laboratories that bring together a variety of disciplines under one roof. DHMRI has developed a multidisciplinary, integrated approach that incorporates a variety of scientific platforms. By combining collaboration and customized solutions, DHMRI finds answers to advance research and product development. MULTIDISCIPLINARY APPROACH No one science or platform can provide the comprehensive analysis needed for confident decision making. That is why DHMRI has adopted and incorporated a multidisciplinary, integrated approach that includes: - Genomics - Proteomics & Protein Characterization - Metabolomics -NMR based Metabolomics - In Vitro Sciences - Light Microscopy - Bioinformatics DHMRI offers the following services that enable a complete research and development experience. 3

4 GENOMICS DHMRI s Genomics Laboratory provides next generation sequencing services, including whole organism de novo sequencing, targeted regional resequencing, DNA methylation profiling by sequencing, and whole transcriptome sequencing. The Genomics Laboratory features multiple platforms for genomic analysis. All services are supported by state-of-the art computational and bioinformatics resources. Applications include: - Next generation sequencing Whole genome sequencing Transcriptome analysis Gene regulation studies Amplicon sequencing - Microarray abilities - Sequenom MassARRAY applications -DNA sequencing Next-generation sequencing Whole genome sequencing SNP detection ChIP-Sequence Transcriptome sequencing Whole genome methylation analysis -Expression analysis -Genotyping Targeted candidate gene expression Whole genome expression analysis Genome-wide association Mid-plex linkage peak/candidate gene analysis Single-plex candidate gene analysis -Small RNA analysis 4

5 PROTEOMICS The Proteomics Laboratory of DHMRI combines advanced instrumentation, high-throughput robotics, and an integrated data management system to provide automated and streamlined solutions for protein biomarker identification and analysis. Through evaluating proteomic changes, researchers can identify and target disease-related biomarkers for disease detection and monitoring as well as potential new drug targets for therapeutic development. Proteomics methods for targeted or discovery based proteomics are provided, through mass spectrometry (MS)-based techniques. The DHMRI Proteomics Mass Spectrometry facility leverages automation, rapid separation systems, and a diverse set of sensitive mass spectrometry instruments to detect, identify, and characterize proteins from purified samples or complex protein mixtures. Applications include: - Protein expression studies leading to diagnostic or predictive markers -Protein and peptide separation by liquid chromatography -Protein/Peptide identification -Intact mass determination -Post-translational modification characterization -Qualitative and quantitative proteomic analysis Open MS-based qualitative and quantitative analysis for differential protein expression and biomarker discovery applications Targeted protein quantitation using LC/MS for biomarker verification and sample screening PROTEIN CHARACTERIZATION The Analytical Sciences Laboratory combines a variety of state-of-the-art instrumentation in a wide assortment of protein characterization experiments. Proteins in complex biological systems may be studied to understand how proteins interact with target molecules as related to disease, diet change, or other biological stresses. Three-dimensional protein structure, protein/ligand binding and protein/protein complex formation, and binding sites may be studied. Dynamic aspects such as protein motion, diffusion properties, and protein/ligand assays and binding constants may also be determined. -Protein ligand binding assays -3D Protein structure determination -Mass spectrometry -Protein dynamics (folding, mobility, size, etc.) 5

6 METABOLOMICS The DHMRI Metabolomics Laboratory provides methods for targeted and broad spectrum analysis using mass spectrometry (MS) and nuclear magnetic resonance (NMR) technologies. Through the linking of metabolites to known biochemical pathways, investigators can develop mechanistic insights that show promise in staging disease, monitoring therapeutic treatments, and developing or improving intervention strategies. Uniquely positioned to provide high throughput metabolite analysis from a single compound to broad metabolite profiling, the Metabolomics Laboratory has the technology and expertise to provide insight into complex biological processes. Applications include: - Custom assay development for small molecule and metabolite quantitation - Metabolite biomarker studies that lead to early predictive or diagnostic markers for: Disease, disease staging, therapeutic monitoring, determining susceptibility -Unknown compound identification and/or structural elucidation -Metabolomics profiling utilizing LC/MS, GC/MS, ICP/MS, and NMR based profiling for high throughput chemical marker discovery -Qualitative and quantitative metabolite analysis -Data analysis and interpretation: Assay development for targeted metabolite detection and quantitation -Structural elucidation using NMR, exact mass, and MS n -Structure determination of other biological/chemical compounds 6

7 IN VITRO SCIENCES DHMRI s In Vitro Sciences Laboratory (IVSL) provides a wide range of protein biochemistry, cell biology, immunology and clinical chemistry capabilities to support a diverse range of discovery, preclinical and clinical research projects. IVSL has experience in a wide variety of disease areas including diabetes, obesity, and cardiovascular disease. By providing integrated biochemistry and cell biology services, IVSL supports the discovery pipeline from basis research through to the human clinical setting. -Genomic analysis -Protein analysis -Cellular analysis Digital gene expression Development of customized curated multiplexed gene panels An intermediate level of biomarker discovery & validation 96- and 384-well plate assays for low-medium throughput profiling Semi-manual, bench top and integrated automation Immunoassay-based capabilities Solid phase time resolved fluorescence Bead-based single- or multiplexed flow immunoassays Plate-based (electro) chemiluminescence Homogeneous immunoassays Gene expression, metabolite, protein and cell analysis (flow cytometry analysis) Multi-parameter flow cytometry for the in-depth quantification Profiling of all major blood cell populations including minor sub-types Quantification of stem cell surface markers Intracellular markers -Clinical biomarker analysis Broad range of ~ 50 clinical chemistries Well-established biomarker panels used in human clinical and diagnostic studies 7

8 LIGHT MICROSCOPY DHMRI s Integrated Microscopy Laboratory (IML) is designed to handle all aspects of microscopy, providing researchers with access to the expertise required to examine subcellular details at high resolution by both light and laser microscopy. IML performs quantitative image analysis for confocal microscopy, fluorescent microscopy, live-cell imaging, sample preparation, and image analysis. IML is located in close proximity to cell culture facilities providing optimal support for live-cell imaging. The wide range of capabilities allows IML to choose the best possible combination of speed, sensitivity and resolution to meet the individual imaging needs of each user. -Consultation on microscopy and techniques -Instruction in microscopy -Access to microscopy equipment for qualified/experienced users -Collaboration on research projects 8

9 BIOINFORMATICS DHMRI has a dedicated bioinformatics team that employs a broad range of analytical tools to analyze the large data sets that are generated in the laboratories. DHMRI Bioinformatics facilitates a collaborative environment using technology and business disciplines for acquiring, managing, integrating, optimizing, analyzing, inferring and referencing disparate data for our Genomic, Proteomic, Metabolomic, and In Vitro sciences laboratories. At the union of science and technology, the bioinformatics group touches every area of scientific research within the DHMRI. Applications include: - Centralized data management - Comparison of novel DNA and protein sequence data to well-characterized data - Intensive data computations within the cluster environment -Genomics Advanced Sequencing QC Analysis Reference mapping/alignments Variant Calling (SNV/SNP) Structural Variation Detection (indels, inversions, CNV, etc.) De novo genomic assembly, finishing, and annotation Metagenomic assembly Transcriptomics - Novel splicing sites, exon, and transcript discovery - RNASeq and Differential Expression Analysis (microarray data as well) - Small RNA analysis - De novo assembly - RNA secondary structure prediction Pathway analysis Targeted Resequencing Analysis Bis-DNA and methylated DNA sequencing analysis ChIP-Seq analysis Phylogenetics 9

10 BLAST/database querying and analysis Genome Wide Association Studies (GWAS) -Proteomics Expression profiling Pathway analysis BLAST/database querying and analysis Pattern discovery Motif discovery Signal peptide discovery Secondary structural determination and prediction Clustering -Metabolomics Pathway analysis Data annotation Multivariate analysis 10

11 CONTACT DHMRI For Business Opportunities, please contact: THE DAVID H. MURDOCK RESEARCH INSTITUTE 150 Research Campus Drive Kannapolis, North Carolina USA

12 THE DAVID H. MURDOCK RESEARCH INSTITUTE FM-0042 R1.0