Welcome into a New World of Chromatography. Karima BAUDIN Oct 2015

Transcription

1 Welcome into a New World of Chromatography Karima BAUDIN Oct 2015

2 Chromatography Portfolio Single and multi-instrument Platform Altus SQ Detector Altus A10 HPLC Altus A30 UPLC NexION LC and GC Speciation Control Software Clarus GC-MS TurboMatrix ATD, HS Arnel Engineered Solutions Clarus GC 580/680 SNFR GC Olfactory Port

3 Introducing Long-term Strategic Partnership 1. Bring class-leading CDS to PerkinElmer Chromatography: Unification of LC and GC for better customer outcomes 2. Complete transformation of LC product line with market leading Technologies. & 3. Unites PKI best in class service and market application knowledge.

4 One single Platforme Software : Controlled under Empower 3 Altus A-10 HPLC Altus A-30 UPLC Altus SQ Detector Clarus 680/580 GC Clarus SQ 8 GC/MS TurboMatrix HeadSpace TurboMatrix Thermal Desorption SNFR Olfactory Port NexION LC and GC Speciation

5 What do we need from an analytical tool? Meet Deadlines Improve Efficiencies Reduce/Mitigate Risk Reduce Costs Increase Confidence in Data/Product Quality Reduce Errors/Manual Entry Control Costs

6 Key Value Propositions Meet Deadlines Improve Efficiencies Built in calculations Tailored calculations Quickly find information with view filters Access to everything needed on a routine basis in one window QuickStart Guided wizards, Show-Me Help Drag and drop reporting to generate what you need, when you need it Reduce review times with the Result Audit Viewer

7 Key Value Propositions Increase Confidence in Data/Product Quality Reduce Errors/Manual Data Entry Reduce/Mitigate Risk Proven, turnkey compliance readiness Full traceability (Database) Track changes to data and methods (Audit Trails) Easily perform searches with view filters Tools for regulatory needs are provided and easily implemented

8 Key Value Propositions Control Costs Reduce Costs One software to Acquire Data Process Data Calculate Results Report Data Laboratory Analytics to Improve Utilization Improve Workflows Identify Resource Needs Identify Training Needs

9 Empower 3 Graphical Dashboard HPLC Data Converter Drag & Drop Reports Automated custom calculations UPLC / UPC ApexTrack GC MS PDA Data collection Data processing Analytics & Reporting View Filters Easily search and track data Dissolution Customized reports Fraction Collection Report Sharing

10 Empower 3 CDS Do more and do it with confidence. Optimal Usability Easy-to-Learn Simple interface specific to each user Advanced processing and reporting capability Greater Value Risk Reduction Reliable data collection and processing Multi-technique CDS (LC, GC, IC, Speciation) Total data traceability Minimal cross-training required Grows with lab

11 The NEW PerkinElmer LC Product Line Altus HPLC A-10 Series of modules featuring technology that's delivered industryleading consistency & confidence for more than 15 years Altus UPLC A-30 Series of modules offering superior chromatographic performance and the lowest dispersion in the industry

12 Wide range of optimized detection techniques A-10 RI A-30 RI Altus SQ A-10 FLR A-30 FLR A-10 UV A-30 UV NexION A-10 PDA A-30 PDA Additional detection options planned AxION 2 TOF

13 Customer Value Propositions: Products CUSTOMER NEED High performance Timely, providing insights predictable and to mitigate risks reliable results Time-critical, high throughput and comprehensive data requirements OUR SOLUTION Altus UPLC Altus HPLC Empower 3 CDS VALUE PROPOSITI ON Provides superior performance with the best throughput and peak resolution in the industry for the most complex chromatographic analyses. Easy-to-use and simplifies lab operations while delivering high performance and consistent, reliable results. Software that delivers data integrity and traceability across multi-vendor instrumentation for the highest degree of confidence to mitigate risk

14 AltusTM HPLC System UV/Vis PDA RI Sample Heating/Cooling FL Vial/Closure options PerformancePLUS degasser Column Heater Column Heater Cooler Switching Valves

15 0.2 0 RT %RSD Area RSD Resolution USP T a b le t T A C Unit Manufactured December, New System a b le t A C R A U System Suitability Criteria R A U Performance Consistency M in u t e s Sample: 0.1mg/mL Irbesartan tablet spiked with 0.1mg/mL Related Compound A (RCA) Column: HSS T3, 5 m, 4.6 x 250mm Injection Volume: 10 L Flow rate: 1.0mL/min Mobile Phase: Isocratic Premix (0.55% Phosphoric acid in Water, ph 3.0):(Acetonitrile) 60:40 Detection: 220-nm, 40 Hz, 0.05 s (normal)

16 Why Altus A-10? Performance Altus-10 systems provide consistent, reproducible results which improves lab productivity gets the job done faster Reliability Users value the design improvements embodied over the years that make Altus-10 systems more rugged and dependable Flexibility Altus-10 systems provide an ideal platform for a broad range of applications using a variety of detection techniques can readily adapt to new needs Regulatory pressures All acquired raw data, results and related instrumental parameters may be securely archived Altus-10 systems come compliant ready Staff turnover in laboratories Users benefit from streamlined daily interactions with Altus-10 systems unattended daily system startups are one example

17 Pulse Free Solvent Delivery The HPLC systems provide unmatched ripple-free solvent delivery enabling lower limits of detection enhancing confidence in your results HPLC System Competitors Traditional Binary System Competitors Traditional Quaternary System 5 ng of the five peptide test mixture on column can be easily distinguished from the baseline in the chromatograms generated on the Separations Module At 5 ng, the peak is lost in the baseline ripple present in the chromatograms generated on a competitive traditional design Binary pump and Quaternary gradient system.

18 Proof of Performance: Gradient Reproducibility Gradient Performance Altus A-10 systems generate unparalleled reproducible gradients that help deliver consistently high quality results increasing lab throughput Tinuvin P MD 1024 BHT BHEB UV 531 Isonox 129 Irganox 3114 Irganox 1010 Irganox 1076 Irgafos 168 Overlay of 12 injections % RSD Rt % % % % % % % % % % % RSD Area % % % % % % % % % %

19 Sampling - Key Performance Attributes : Linearity

20 Sampling - Key Performance Attributes : Automated System Start-up SystemPREP Alliance/Altus A-10 system fluidics can be fully primed/purged/equilibrated from the front panel or automatically, unattended via software control

21 Sampling - Key Performance Attributes Automated System Start-up Automation benefits User can easily build unattended daily startup routines into sample methods with their A-10 systems frees up valuable lab time

22 Major Benefits of the Altus A-10 System SystemPREP eases daily startup routines 4-solvent AutoBlend capability Active plunger seal wash Pulse-less solvent delivery Excellent gradient performance Minimized carry over Column temperature control Tool-free routine maintenance

23 AltusTM UPLC System Detectors A-30h Column Heater with Active Pre-Heating TUV, e PDA, FLR, RI LCMS A-30 Sample Module Sample Manager- Flow Through Needle A-30 Solvent Module Quaternary Solvent Manager

24 What Does It Do? Provides the power of sub 2 micron particles with the flexibility of multi-solvent mixing Provides tools to transfer HPLC methods as HPLC or as UPLC methods, as necessary Familiar flow path for injection and multisolvent delivery at 15,000 psi allows the ability to use HPLC SOPs in place today, as well as transitioning to UPLC in timely fashion

25 A-30 Quaternary Solvent Module Operating characteristics Maximum flow = 2 ml/min Maximum operating pressure = 15,000 psi Quaternary solvent delivery Continuous blending of four solvents in any combination or proportion Eliminates potential variability caused by manual mixing of solvents Optional integrated Solvent Select Valve expands solvent choices

26 A-30 Sample Module w/ FTN Programmable volume injection Up to 10 ul injection standard Up to 250 ul with extensions Needle in flow path design Total volume sample injection Full range of plates and vials Supports load ahead and loop offline For increased throughput

27 UPLC System Detection: Tunable UV PDA /e PDA Fluorescence Refractive Index Single Quad MS Software Control*: Empower 3 HPLC Compatible Column Management: Heating Heating/Cooling Column Switching ecord Technology Sample Module Quaternary Solvent Module

28 Tunable Ultraviolet Detector (TUV Detector) HPLC TUV Baseline Noise - <5.0 E-6 ASTM Procedure E Linearity - 5% at 2.5 AU Linear Range Optical Bandwidth 5nm Accuracy Wavelength Range nm Data Rate Single Wavelength 80 Hz Dual Wavelength 2Hz UPLC TUV Baseline Noise - <6.0 E-6 ASTM Procedure E Linearity - 5% at 2.5 AU Linear Range Optical Bandwidth 5nm Accuracy Wavelength Range nm Data Rate Single Wavelength 80 Hz Dual Wavelength 2Hz

29 TUV Functionalities Lamp Optimization Software Maximizes s/n performance regardless of lamp age or wavelength Extends the useful life of the lamp Ensures reproducibility Allows the use of a single lamp over the entire operational wavelength range No need to monitor energy from 2 lamps Integral Ebrium Filter Used for internal calibration upon startup using 4 points across entire spectrum

30 Photo-Diode Array Detector (PDA Detector) HPLC PDA UPLC PDA Wavelength Range nm Wavelength Range nm Number of Diodes 512 Number of Diodes 512 Slit Width 1.2nm Fixed Slit Width 1.2nm Fixed Sampling Rate 80Hz Maximum Sampling Rate 80Hz Maximum 2D Channels Maximum of 8 2D Channels Maximum of 8 Noise 1.5 E-6 230nm Noise 1 E-6 254nm Linearity - 5.0% at 2.0AU Linearity 5.0% at 2.0AU

31 High Brightness Lamp (TUV and PDA) High brightness deuterium lamp Low noise characteristics Ability to output nearly twice the brightness (energy) of ordinary deuterium lamps Benefits Less Noise Higher sensitivity Lamp Warranty 2000 hours Front Accessible Ease-of-Use Lamp Information in Console Tracked on Chip o Hours of operation o Ignitions o Serial Number Benefit: High Sensitivity

32 Fluorescence Detector (FLR Detector) HPLC FLR UPLC FLR Excitation nm Excitation nm Emission nm Emission nm Optical Bandwidth 20nm Optical Bandwidth 20nm Data Rate 80Hz Data Rate 20 Hz (1Hz in 3D) 2D Channels 4 2D Channels 4 Range ,000 EU Range ,000 EU Lamp High Energy Xenon/Mercury Lamp Xenon

33 UPLC FLR Low Dispersion Flow Cell Axially Illuminated Flow Cell The cell entrance (lens) and opposing wall are optically matched allowing energy to be reflected back for a second opportunity to be absorbed. Double pass design effectively doubles the path length resulting in increased sensitivity. Light is either absorbed on the second pass or exits through the lens reducing scatter. The narrow rectangular shape disperses liquids less than traditional cubic flow cells.

34 UPLC FLR 3D Capabilities for Method Development 3D Scan For on the fly spectra scanning λ-λ Plot Simultaneously scan excitation and emission wavelength Console Scan Independently scan excitation and emission wavelength Requires static sample (cuvette) Requires static sample (cuvette) Best choice for unknown conditions Best choice for known compounds Similar to PDA functionality Scan excitation and emission separately

35 Refractive Index Detector (RI Detector) UPLC RI HPLC RI RI Range RIU Noise E-9 RIU Linearity - 5.0% over ±5.0 E-4 RIU Lamp 870 nm LED Temperature Control Internal Oven 30 to 55 C Flow Cell Pressure Limit psi RI Range RIU Noise - ±1.5 E-8 RIU Linearity - 5.0% over ±5.0 E-4 RIU Lamp 870nm LED Temperature Control Internal Oven C External Column Heater Ambient 150C Flow Cell Pressure Limit psi

36 Refractive Index Features High Intensity Lamp Long Lifetime 870 nm Thermal Management Counter current heat exchanger Integrated Fluidics Automated solvent recycling and reference flow cell purge Data Acquisition Sampling Rate of 1 80 Points/Second Independent Optimization of sampling rate and filter time constants

37 Customer Value Propositions: Products CUSTOMER NEED High performance Timely, providing insights predictable and to mitigate risks reliable results Time-critical, high throughput and comprehensive data requirements OUR SOLUTION Altus UPLC Altus HPLC Empower 3 CDS VALUE PROPOSITION Provides superior performance with the best throughput and peak resolution in the industry for the most complex chromatographic analyses. Easy-to-use and simplifies lab operations while delivering high performance and consistent, reliable results. Software that delivers data integrity and traceability across multi-vendor instrumentation for the highest degree of confidence to mitigate risk

38 A New Day in chromatography