Evaluation of Enzymatic Hydrolysis Process of Sugarcane Bagasse

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1 Evaluation of Enzymatic Hydrolysis Process of Sugarcane Bagasse FAPESP-OXITENO LABORATORY OF INDUSTRIAL BIOTECHNOLOGY CENTER FOR TECHNOLOGY OF PROCESSES AND PRODUCTS

2 The Institute for Technological Research of the State of São Paulo

3 IPT as a Conector Universities Basic and Applied Research Education Extension Programs IPT Pre-competitive research Technological Development Management of cooperative projects Technological Services Translator of business demands Companies Products and Services Financial Resources R&D

4 Infrastructure IPT units in: São Paulo Franca (leather and shoes) São José dos Campos* (composite materials) Piracicaba* (biomass) * in progress 12 technology centers 40 laboratories ,00 m 2 of built area m 2 of total area

5 Evaluation of Enzymatic Hydrolysis Process of Sugarcane Bagasse OBJECTIVES Evaluate the conditions of steam explosion of sugarcane bagasse to improve the susceptibility to enzymes Evaluate the conditions of delignification of sugarcane bagasse with NaOH or H 2 O 2 that increase its susceptibility to enzymes Kinetic study of enzymatic hydrolysis of pretreated bagasse: substrate/cellulase ratio, agitation, influence of delignification on pretreated bagasse.

6 Steam Explosion Pretreatment In Natura Bagasse (Characterization) Steam Explosion Pretreatment 180 C for 5, 15, 30 and 50 minutes 195 C for 3, 6, 9, 15, 20 and 30 minutes 210 C for 1, 2, 4, 6, 9 and 12 minutes Hemicellulosic Hydrolysed (Characterization) Pretreated Bagasse (Characterization) Enzymatic Hydrolysis Standard condition with commercial enzimes complexes. Tests with xylanase suplementation. Delignification Pretreatment Standard condition with NaOH. Lignin solubilization was evaluated. Pretreated and Delignified Bagasse (Characterization) Glucose yield

7 Steam Explosion Pretreatment A B C D E F G Fig. 2: Images of in natura bagasse (A) and pretreated bagasse at 210ºC for 1 min (B), 2 min (C), 4 min (D), 6 min (E), 9 min (F) and 12 min (G). Fig. 1: Steam explosion system for biomass pretreatment In Natura Pretreated

8 Preliminary Delignification of Pretreated Bagasse Figure 3 Figure 4

9 Pretreated Bagasse and Pretreated/Delignified Bagasse Cellulose Hemicellulose Lignin Acetyl groups Cellulose Hemicellulose Lignin Time (min) Time (min) Figure 5 - Effect of pre-treatment time at 180 o C in the mass of recovered components from (A) pretreated bagasse and (B) pretreated and delignified bagasse (100 C 1h ). BIN: In Natura Bagasse

10 Hemicellulose (g/kg BIN) Hemicellulose (g/kg BIN) Hemicellulose (g/kg BIN) Hemicellulosic Hydrolyzed ºC 195ºC 210ºC time (min) X, XO, A, F (g/kg BIN) time (min) Figure 6 - Chemical composition of hemicellulosic hydrolyzed. hemicellulose, xylo-oligomers (XO), xylose (X), arabinose (A), furfural (F), soluble lignin (LS) X, XO, A, F (g/kg BIN) time (min) X, XO, A, F (g/kg BIN)

11 Rendimento Glucose em yield glicose (%) (g/100g Glic. Pot.) Rendimento em glicose (g/kg BIN) Pretreatment: Enzymatic Hydrolysis 100,0 90,0 80,0 70,0 60,0 solid line = 180 C (9 bar) 300,0 dashed line (A) = 195 C (13 bar) dotted line = 210 C (18 bar) = pretreated bagasse 250,0 = pretreated bagasse plus xylanase = pretreated/delignified bagasse 200,0 50,0 40,0 30,0 20,0 10,0 150,0 100,0 50,0 0, time (min) Figure 7 Influence tempo of bagasse (min) pretreatment conditions on enzymatic hydrolysis. 0,

12 Kinetic of Enzymatic Hydrolysis 2 nd Phase Kinetic study in a 40 L stirred reactor Objective: Evaluation of hydrolysis conditions on sugar yield. Parameters Substrate: pretreated and pretreated/delignified bagasse Agitation Enzyme charge: up to 60 FPU/g cellulose Bagasse concentration: up to 25% (w/w) Xylanase supplementation

13 BIOREFINERY RELATED PROJECTS Lactic Acid Production from Hemicellulosic Hydrolyzed Objective: Isolation and screening of bacteria for producing lactic acid consuming xylose from hemicellulosic hydrolyzed.

14 Team Alfredo Eduardo Maiorano Chemical Eng. (Dr.) Maria Filomena de A. Rodrigues Biochemical Pharm. (Drª) Marcelo Brant Whurthmann Saad Biochemical Eng. (Msc) Eliza Mami Ota Food Eng. (Msc) Luiz Pereira Ramos technical advisor Ivan Fabian Arcuri Chemical eng. Luiz Roberto Magossi - technician Valter de Oliveira - technician Paula Cristina Frausto - trainee Monique Belniowski - trainee

15 Thank you Maria Filomena de Andrade Rodrigues twitter.com/iptsp