Abstract Book. National Seminar on. Plants and Environment (NSSAPPE-2017) March 27 & 28, Organized by Department of Botany, D. S. B.

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1 Abstract Book National Seminar on Plants and Environment (NSSAPPE-2017) March 27 & 28, 2017 Organized by Department of Botany, D. S. B. Campus, Under SAP, UGC Programme Kumaun University, Nainital 1

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4 About the University Kumaun University was established in It consists of three campuses at Almora, Nainital and Bhimtal. More than 100 affiliated government colleges and self-financed private institutions spread across the Kumaun region. In the affiliated campuses, both teaching and research activities are equally important factors that support the curriculum and contribute to the student's learning habits. NAAC accredited A grade to Kumaun University in About the Botany Department The Botany department was established in 1951 as a government postgraduate college and since 1973, it is functioning as a department of University. The department has been graced by many nationally and internationally known scientists/ teachers including Prof. K.S. Bhargava, Prof. S.C. Gupta, Prof. B.S. Mehrotra, Prof. J. S. Singh, Prof. S.P. Singh, Prof. R.D. Khulbe, Prof. Y.P.S. Pangtey, Prof. Gira Bala Pant, Prof. Sudhir Chandra and Prof. Uma Palni. Prof. J.S. Singh was awarded FNA and Shanti Swaroop Bhatnagar prize. Prof. S.P. Singh was awarded FNA and Birbal Sahni medal and also worked as Hon ble Vice Chancellor, Garhwal University, Srinagar. Prof. Y.P.S. Pangtey, NASI fellow was awarded FNASc and worked as UGC, Emeritus Fellow. The department has been included under FIST programme, DST, New Delhi and recognized as DRS (SAP) department, UGC New Delhi. The department actively collaborated with major institutions like G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi, Katarmal- Almora, NBRI Lucknow, NBPGR Bhowali, CIMAP, Lucknow, BSI, Dehradun, RRIHF, CCRAS, Tarikhet and CAP, Selaqua. A large number of research projects and several collaborative research programmes involving various national and international agencies have been completed and ongoing. Over 500 research papers have been published in more than 50 journals of national and international repute and about 250 students have been awarded Doctoral degree in past two decades. Specimens of Angiosperms (2500), Pteridophytes (300), Bryophytes (>200), Fungi (>100) and Lichens (nearly 100) have been preserved in herbarium. Presently semester courses at undergraduate, postgraduate and course system Ph. D. are being run in the department. At Post Graduate level, specializations are running in various disciplines viz., Forest Ecology, Bryology, Plant Pathology, Ethonobotany and Plant Taxonomy. The department is regularly publishing an annual news letter OAKS for last ten years. The department has well equipped and computerized laboratories of Plant Tissue Culture, Plant Pathology and Aquatic Mycology, Ecology, Taxonomy and Bryology. At present, the 4

5 department has two glass houses, one mist chamber to carry out various green house experiments and research activities. The major equipments available in the department for research activities are Spectrophotometer, Pressure chamber, Laminar air flow chamber, ultra cool centrifuge, lypholizer, BOD Incubator, seed germinator, LCDs, Mico Kjeldhal unit, Microscopes, Xerox machines, Research Microscopes, Multi media facility, Fax facility etc. About the National Conference The Botany Department, D.S.B. Campus Kumaun University, Nainital, Uttarakhand, India is organising a National Conference on 27 & 28 March 7 The plenary, key notes and invited talks by eminent Scientist of this region would give insight to the current research scenario for the benefits to the society. The seminar will cover the following areas: Systematic Biology Drug Design Natural Product Ecosystem Environment Biotechnology Microbiology Botany Department of Nainital was established on 1951 and reorganised in 1973 with the establishment of the Kumaun University, Nainital. The world class research centre of Uttarakhand working in the several of Plant Sciences. This conference is organised under the SAP- UGC programme of the Department. 5

6 Patron Hon ble Vice Chancellor, Kumaun University, Nainital Chairman Prof. S.P.S. Mehta, Director D. S. B. Campus, Nainital Co-Chairman Prof Neerja Pande, Head, Department of Botany, D.S.B. Campus, Nainital Convener Prof. S. C. Sati, Department of Botany, D.S.B. Campus, Nainital Co-convener Prof. Y. S. Rawat, Department of Botany, D. S. B. Campus, Nainital Organising Secretary Prof. Lalit Tewari, Department of Botany, D. S. B. Campus, Nainital Joint Secretary Dr. Kiran Bargali, Dr. Sushma Tamta Dr. Neelu Lodhiyal, Dr. Anil Bisht, Dr. Kapil Khulbe, D. S. B. Campus, Nainital Advisory Board Prof. Santosh Kumar, Dean Science, Prof. P. C. Pande, Prof. Hema Joshi, Prof. S. S. Gahlan Prof. S. S. Bargali Members Dr. Yogesh Joshi, Dr. Balwant Kumar, Dr. Dhani Arya, Dr. Ashish Tewari, Dr. Veena Pande, Sri Yugal Joshi, Sri Suchetan Sah, Dr. Geeta Teawri, Dr. Tapan Nailwal, Dr. Santosh Upadhyay, Dr. Penny Joshi, Dr. Himanshu Lohani, Dr. Sohail Javed, Dr. Vijay Kumar, Dr. Lalit Mohan National Advisory Committee Prof. D. K. Maheshwari, Haridwar, Prof. A. K. Pandey, New Delhi, Prof L. M. S. Palni, Dehradun, Prof. Y. P. S. Pangtey, Haldwani, Prof. Sudhir Chandra, Nainital Prof. Uma Palni, Dehradun, Dr. Gopal Rawat, Dehradun, Dr. S. S. Samant, Mohal, Kulu Prof. Jeet Ram, Nainital, Prof. L. S. Lodhiyal, Nainital, Dr. Jyoti Saxena, Dwarahat, Prof. Uma Melkania, Pantnagar, Dr R.S. Rawal, Katarmal, Prof P. B. Rao, Pantnagar, Dr G. C. Joshi, Tarikhet, Dr. B. S. Kalakoti, Haldwani, Dr. I. S. Bisht, Niglat, Prof. Satish Garkoti, J. N. U., New Delhi, Dr. D. K. Upreti, N. B. R. I., Lucknow, Prof. R. C. Dubey, Haridwar, Prof. S. V. S. Chauhan, Agra, Dr. Rajnish Agnihotri, Agra, Prof. N. N. Tripathi, Gorakhpur, Prof. Chitra Pande, Nainital, Prof. N. K. Dubey, Varanasi, Prof. V. P. Singh, Delhi, Prof. Arun Arya, Barodara, Dr. Virendra Nath, Lucknow, Prof. N. S. Bisht, Pauri, Prof. Ganga Bisht, Prof. Suchi Bisht, Prof. Sanjay Pant, Prof. C. K. Pant, Dr. N. G. Sahoo, Nainital, Dr. P. B. Tewari, Chandausi Speakers Prof. Nageshwar Rao, Prof. S. P. S. Mehta, Prof. Santosh Kumar, Prof. Uma Melkania. Dr. B. S. Kalakoti, Dr Amit Pandey, Dr. I. D. Bhatt, Dr. G. C. S. Negi, Prof. Neerja Pande, Prof. 6

7 S. C. Sati, Prof. Ganga Bisht, Prof. P. C. Pande, Prof. Lalit Tewari, Dr. Ashish Tewari, Dr. Yogesh Joshi Panel of experts Prof. Sudhir Chandra, Dr. S. D. Tewari, Dr. Amit Pandey Contacts Prof. S. C. Sati Prof. Y. S. Rawat Prof. Lalit Tewari Convener Co Convenor Organising Secretary Mob.: Mob.: Mob.:

8 Abstracts 8

9 Index S. No. Name of Participant Title Page no. 1 Bharat Giri Flow of Forest Ecosystem Services with Special Emphasis on C- 12 Gosain Sequestration in a Mountain Watershed, Kumaun Himalaya 2 Vijay Arya Ecosystem Services in Temperate Sacred Forests of Kumaun Himalaya 13 3 Rajni Rawat, Distribution and Diversity of Ocimum Species Occurring in Different Agro- 14 Ecologies of Uttarakhand State 4 Kamla Dhyani Allelopathic Effect of Leaf Extract of (Poplar Deltoidus) in Seed Germination, Root Characters and Physiology of Rice Seedlings 15 5 Niraj Singh Development and Application of Scar Markers for Identification of 16 Gladiolus Germplasm 6 Harsh Chauhan Rapid Vulnerability Assessment of Trillium Govanianum Wall. Ex D. Don; 17 A Commercially Exploited Medicinal Plant of Himalaya 7 Indu Tiwari Impact of Environmental Factors on the Growth of Plants 18 8 Prachi Tamta Observation on Status of Insect Pollinators in Binsar Wildlife Sanctuary, 19 Western Himalayas, Uttarakhand, India 9 Mahesha Nand Qsar Based Virtual Screening of Novel Phytochemical Inhibitors Against 20 Her2 in Breast Cancer 10 Preeti Bhatt Biochemical Aspects of Photosynthesis and Respiration: An Implication to 21 Environment and Social Awareness 11 Meenakshi Negi Analysis of Forest Vegetation, Population Structure And Regeneration 22 Pattern In Kumaun Himalaya 12 Hemlata Rathore A Seasonal (Rainy Season) Survey and Study on the Availability of Different Fodder Species of Kumaun and Garhwal Region of Uttarakhand Prabha B. Allelopathic Effect of Bryophytes on Germination and Growth of Bidens 24 Dhondiyal Biternata Lour.) Merr & Shreff 14 Netrapal Sharma Genetic Diversity among Sea Buckthorn (Hippophae Rhamnoides (L)) using Molecular Marker based Analysis 15 Balwant Kumar Impact of Lichen Litter on Soil Nutrients in the Temperate Areas of Kumaun Himalaya (Uttarakhand) 16 Mahesh Adsorptive Removal of Zinc (II) by Activated Bark of Pinus Roxburghii : Chandra Arya An Ecofriendly Approach 17 Uzma Siddiqui Observation on Environmental Effect on the Breeding Dynamics of Schizothorax Richardsonii (Gray), from Kumaun Himalayas, Uttarakhand, India 18 Pushpa Bhakuni Antimalarial activities of some Plant Species Found in Kumaun Region Uttarakhand 19 Sapna A Review of Chemical Analyses and Ethno-Medicinal Uses of 30 Zingiberaceae Family 20 Mamta Bisht Effect of Zinc on Growth and Herbage Yield of Ocimum Gratissimum L Bhanu Kumar Chemotypic Variations in Ageratum Conyzoides L. Collected from Indo- 32 Gangetic Plains 22 Akanksha Rani Essential Oil Composition of Murraya Koenigii Collected from Three 33 Locations of North India 23 Brij M. Upreti Physico Chemical Characterization of Soil Collected From Sacred and Non 34 Sacred Forests of Uttarakhand: A Comparative Study 24 Vandana Jaggi, Confirmation of Interacting Partners of Chickpea DNA Methytransferases Clones and their Interaction With Ago Protein

10 25 Deepshekha Punetha Heavy Metal Accumulation by Coriandrum Sativum in Cow Dung Amended Soils 26 Sunita Bhatt Oven Dried Volatile Constituents of Ocimum americanum: A Rich Source 37 of Bioactive Compounds 27 Jitendra Bhatt Changes in Biodiversity and Regeneration Pattern in Plant 38 Communities after Eradication of Lantana Camara L. From the Forest Areas of Uttarakhand 28 Nandan Singh Tree Water Relation and Regeneration Status of Treeline Species in 39 Tungnath Area of Uttarakhand 29 Shruti Shah Assessing Regeneration Potential and Developing Reliable Indicators 40 of Seed Maturity in Myrica esculenta, Buch-Ham. Ex D. Don, in Kumaun Central Himalayan Region 30 Bhawna Tewari Developing Nursery Techniques for Propagation of Prunus cerasoides 41 D. Don 31 Pritee Pant Antioxidant and Phytochemical Analysis of two Medicinal Plants of 42 Kumaun Himalaya 32 Neeta Arya Diversity Analysis in Pinus Roxburghii Sarg. and Mixed Broadleaf Forest of 43 Central Hiimalaya, India 33 Neha Rajwar Solid Waste Management through Vermicomposting and Earthworm Gut 44 Cellulolytic Bacteria 34 Poonam Prasad A Comparison of Canopy Cover Measured through Transect and 45 Densiometer Methods in Oak Forest of Central Himalaya India 35 Rajesh Kumar Zirconium Doped Iron Oxide Nanocomposite for the Adsorption of Cd (Ii) : 46 An Eco-Friendly Protocol 36 Jeewan Gahtori A comparative study on water quality of river Saryu for drinking purposed 47 in Bageshwer District 37 Anita Rana Density relationship for adsorption capacity of Liriodendron tulipifera (hard 48 wood) and Cedrus deodara (soft wood) 38 Urvashi Verma Quantitative estimation of total Phenolic content, total flavonoid content and 49 free radical scavenging potential of Himalayan Fir (Abies pindrow (Royle ex D.Don) Royle) : An Ethnomedicinally Important Plant 39 Krishna Kumar Economic Upliftment of Local Communities through Cheura (Diploknema 50 Tamta Butyracea Roxb.) Plantations in Kumaun 40 KaminiTamta Biomass Status In Ungrazed And Grazed Oak Forest Grazingland In The 51 Himalaya, India 41 Naveen Ethnobotanical Studies of Betalghat Region, along with Kosi Catchment 52 Chandra Pandey Area, Kumaun Himalya 42 Sumita Rana Cultivated Exotic Medicinal Plants in Tarai Belt of Uttarakhand, India Prabha Pant Screening for Phosphate Solubilization Potential of Two Rhizospheric Fungi Parikshit Kumar Phytochemical analysis and evaluation of Kumaun Himalayan Litsea 55 umbrosa Nees bark extract for its antimicrobial and antioxidant potentiality 45 Amit Mittal Impact of Winter Water Stress on Seedlings of Quercus Leucotrichophora 56 A.Campus and Rhododendron Arboreum Wall 46 Kanchan Upreti Ethnobotany and Indigenous Uses of Urtica dioica L. in Uttarakhand 57 Himalaya 47 Geeta Tewari Essential Oil Composition and Antifungal Activity of Pimpinella 58 Achilleifolia (DC) Clark 48 Neelaxi Pandey Nutraceutical Plant Diversity in Kumaun Himalaya of Uttarakhand, India Pankaj Kumar Biotechnological Traditional Knowledge and Related IPR Issues Pramod Kumar Litter Decomposition in a 18-Year Old Holoptelea Intrigrifolia Planch Plantation in A Sub-Tropical Forest of Tehri Garhwal

11 51 Priyanka Maiti Exploration of Plant Derived Neutraciticals against Lung Cancer Using In 62 Silico Tools 52 Rashi Miglani Ecotoxic Investigations on Agro Ecosystems with Special Reference to 63 Earthworms 53 Renu Suyal Epiphytic Orchids and Their Host Species in Baliyanala Catchment Area of 64 Nainital, Kumaun Himalaya 54 Ripu Daman Impact of Forest Fires on Stand Structure and Regeneration 65 Singh 55 Shalini Rawat Radiation Absorber Plants Surabhi Gumber Fire Adaptations in Some Important Tree Species Vinay Singh Study on the Ecological Importance of Aquatic Avian Fauna of Wetlands of 68 Kumaun Region, India. 58 Yogesh Joshi New Horizons in the Field of Lichenology: Case Study from Kumaun Himalaya Bhaskar Ch. Watershed, West Himalaya, India 70 Joshi 60 Ira Tewari Significance of Community Participation in Promoting the Conservation of 71 Musk Deer and Its Preferred Fodder in Uttarakhand, In Uttarakhand Himalaya 61 Jagmohan Singh Negi Synthesis of Some New Azetidinones Derivatives with Possible Biological Activities 72 11

12 1. Flow of Forest Ecosystem Services with Special Emphasis on C-Sequestration in a Mountain Watershed, Kumaun Himalaya Bharat Giri Gosain G.B. Pant National Institute of Himalayan Environment and Sustainable Development (GBPNIHESD) Kosi-Katarmal, Almora , India. bharatgirigosain@rediffmail.com A major problem being faced by human society is that the global temperature is believed to be rising due to human activities that release CO 2 and other greenhouse gases to the atmosphere and thus causes global warming. In atmosphere CO 2 accounts for 0.035% and it is the most abundant greenhouse gas present in the atmosphere. Besides this other greenhouse gases responsible for global warming are methane, nitrous oxide, ozone, water vapours and CFCs. C-sequestration is the process through which agriculture and forestry practices remove CO 2 from the atmosphere. The forests have potential to capture and retain large volumes of carbon over long periods as trees absorb carbon through photosynthesis process. The present study was conducted in Syahi Devi forests located in a mountain watershed (Kosi) Almora district of Uttarakhand, aimed to assessed the potential of C-sequestration rate and Net Primary Productivity (NPP) of Oak (Q. leucotricophora) and Pine (P. roxburghii) forests at an altitude of m. C-sequestration rate was estimated following McDicken (1997) and Tewari et al. (2008). NPP was following Chaturvedi and Singh (1987) and Rawat and Singh (1988) by using the allometric equations. Total tree layer biomass in the first year in Oak forests (mean = t/ha) increased in next year (mean = t/ha) in Average NPP of tree layer of Oak forests in the present study was thus computed to 6.81 t/ha/yr. In Pine forests tree layer biomass (mean = t/ha) in year 2014, increased (mean = t/ha) in 2015, thus NPP of Pine forests tree layer was computed to be t/ha/yr. C-sequestration rate in Oak and Pine forests in the present study was recorded 3.23 t/ha/yr (range = t/ha/yr) and 5.61 t/ha/yr (range = t/ha/yr), respectively. Key word: C-sequestration, greenhouse gases, net primary productivity, mountain watershed, Kumaun Himalaya. 12

13 2. Ecosystem Services in Temperate Sacred Forests of Kumaun Himalaya Vijay Arya and G. C. S. Negi* Department of Botany, S.S.J. Campus Almora , Kumaun University, Uttarakhand G. B. Pant National Institute of Himalayan Environment & Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand Sacred forests are a group of trees or a patch of vegetation protected by the local people through religious beliefs and socio-cultural regulations practices.uttarakhand has a rich history of conservation of forests through this approach. The present study was carried out in eight temperate sacred forests of Pithoragarh district, Kumaun Himalaya situated between masl.these forests have been offered to the local deity (folk god and goddess) and declared as sacred forest by the local communities either to stop extraction of forest resources or regulate their use framing well-defined rules and regulations. The eight sacred forests we studied have been protected for the last 5-10 years. A questionnaire based survey was conducted in the adjacent villages of these forests to get information about the ecosystem services provided by these sacred forests. People acknowledged use of various ecosystem goods and services from these forests and understood the benefits of protecting these forests which are now providing intangible services such as water, control of soil erosion, wildlife conservation, moderation of temperature, aesthetic value etc. This paper describes various mechanisms employed by the rural people to protect their forests through the use of religious and socio-cultural ethos that is contributing to biodiversity conservation and sustainable utilization of the forest resources. Keywords: Ecosystem services, sacred forests, Pithoragarh, Kumaun Himalaya 13

14 3. Distribution and Diversity of Ocimum Species Occurring in Different Agro- Ecologies of Uttarakhand State Rajni Rawat, Ranjana Singh, K.S. Negi, P.S. Mehta, S. K. Verma and I. S. Bisht ICAR-National Bureau of Plant Genetic Resources, Regional Station, Bhowali (Nainital), Uttarakhand , India Deceased on 14 December 2016 Basil is an aromatic and sacred herb in the family Lamiaceae, used both as a classic culinary, ornamental and cosmopolitan herb of home gardens. The genus Ocimum contains approximately 65 species of herbs and shrubs originated in tropical Asia. In the present study diversity of 79 accessions has been described. Of the 79 accessions, 51 accessions were collected indigenously from the farmer households of eight districts of Kumaon and Garhwal region of Uttarakhand state viz. Almora, Bageshwar, Chamoli, Champawat, Nainital, Pauri, Pithoragarh and Rudrapryag, and 28 accessions were augmented from exotic sources. Six species of Ocimum namely O. basilicum (six varieties i.e. O.b. var pilosum, O.b. var basilicum, O.b. var glabratum, O.b. var difforme, O.b. var thyrsiflora and O.b. var purpurascens), O. tenuiflorum, O. gratissimum, O. Virde, O. kilimandascharium and O. citriodorum were represented in the study. The accessions were characterized in the Kharif seasons of and at ICAR-NBPGR Regional Station- Bhowali, Nainital, Uttarakhand for quantitative and qualitative morphological characters including essential oil yield content and composition using standardized descriptors for identification of superior genotypes. Wide variations were recorded for different quantitative characters such as spike length, plant height, herbage yield, oil percentage and major component of essential oil. Spike length varied from cm, plant height varied from cm, herbage yield varied from g, oil percentage varied from %. Methyl chavicol, linalool, β- cedrene, β- bisabolene, 1,8- cineole and eugenol were recorded as major compound of above mentioned Ocimum species. Some promising accessions identified for major compound in different species include, O. b. var. pilosum (EC ) for methyl chavicol (88.6 %); O. b. var. basilicum (EC ) for linalool (82.4%); O. virdie (NRT-05) for β bisabolene (30.25%); O. kilimandscharicum (IC ) for camphor (45.58%), and O. tenuiflorum (RNRT- 26) for eugenol (70.52%). Promising accessions for herbage yield include O. b. var. basilicum (EC , 710 g) and O. citriodorum (NRT- 03, g). These promising accessions have potential for commercial cultivation in different parts of Uttarakhand hills. Keywords: essential oil, Ocimum, quantitative genetic variations, species 14

15 4. Allelopathic Effect of Leaf Extract of (Poplar Deltoidus) in Seed Germination, Root Characters and Physiology of Rice Seedlings Kamla Dhyani, Namrata Singh, Chhaya Singh and Shweta Sahni Shri Guru Ram Rai Institute of Technology & Sciences, Patel Nagar Dehradun, Uttrakhand In the present investigation an attempt to be made to analyse the potential allelopathic effect of Poplar deltoidus leaf extract at different concentrations (0, 1, 2, 5 and 10 %) of water extract of green and decomposed leaves of Poplar deltoidus. On seed germination, seedling growth and biochemical constituents changes in Oryza sativa L. The results showed that the both leaves extracts of Poplar caused a general phytotoxic effect on seed germination and seedling growth of O. Sativa at some concentration like 10 % and 5 % and some stimulatory effect on some lower concentrations (1% and 2 %). It was noticed that the germination % in both the extract is inhibited in higher concentration. The degree of seed germination inhibition was higher in green leaf extract as compare to decomposed leaf more than. It was also noticed that root morphology is highly effected by the treatment extensive secondary root growth was observed in lower concentration extract as compare to the control. Allelopathic effect on germination percentage is not significant but the other growth parameters like shoot length, root length, dry weight, chlorophyll content of the leaves and proline content is highly effected. 15

16 5. Development and Application of SCAR Markers for Identification of Gladiolus Germplasm Niraj Singh 1, Ashish K. Pal 1, Baleshwar Meena 1, R. K. Roy 1, Sushma Tamta 2 and T. S. Rana 1 1 CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow , India 2 Department of Botany, D. S. B. Campus, Kumaun University, Nainital , Uttarakhand, India niraj.singh37@gmail.com Gladiolus is an economically important ornamental crop, cultivated for its beautiful flowers throughout the world. The genus Gladiolus consists of about 265 species and is one of the largest genera in the family Iridaceae. As the number of Gladiolus cultivars are increasing day by day in India, therefore, it is very significant to develop cultivar specific markers for rapid identification. The correct genotype identification of plant material is very significant in the floriculture industry. The aim of this study was to develop sequence-characterized amplified region (SCAR) markers from the RAPD and ISSR fragments for identification and authentication of Gladiolus germplasm. The SCAR markers developed could be easily employed as valuable tools to identify newly developed Gladiolus cultivars. The SCAR markers viz., ScG12, ScG34 and ScG36 are specific to the DNAs from all sixty two Gladiolus cultivars, as they did not amplify the DNAs of other taxa of the family Iridaceae, including Iris, Amaryllis, and Narcissus. All three SCAR markers distinguished Gladiolus from other taxa of the family Iridaceae, whereas marker ScAm was specific to Amethyst cultivar. Our results confirmed that this particular SCAR marker distinguished Amethyst cultivar from rest of other sixty two Gladiolus cultivars investigated in the present study. The development of SCAR markers based on RAPD and ISSR markers seems to be the maiden attempt for Gladiolus cultivars. Keywords: Gladiolus, identification, ISSR, RAPD, SCAR marker 16

17 6. Rapid Vulnerability Assessment of Trillium Govanianum Wall. Ex D. Don; A Commercially Exploited Medicinal Plant of Himalaya Harsh Kumar Chauhan 1,2, Anil Kumar Bisht 1, Indra Dutt Bhatt 2, 1 Department of Botany, D.S.B. Campus, Kumaun University, Nainital , India 2 G.B. Pant National Institute of Himalayan Environment & Sustainable Development, Kosi-Katarmal, Almora , India bishtakb@rediffmail.com Trillium govanianum Wall. ex D. Don (Nagchhatri) is an endemic Himalayan medicinal plant restricted to shady, moist and cold habitat in subalpine zones at m asl. Trade of its rhizomes collected from wild populations has recently emerged as a significant income source in Himalayan region. The rhizome of the species is reported to have rich amount of steroidal saponins and have potent uses in cancer, reproductive disorders and inflammation. We assessed ecological vulnerabilities in 17 sites from Tirthan Valley (Himachal Pradesh), Munsiyari and Tunghnath (Uttarakhand); and socio-economic vulnerabilities through communal focus groups and interviews with 579 gatherers and 19 traders. Species vulnerability was recorded highest in Munsiyari, then Tunghnath, and indicated that the species may reach at the brink of extinction in near future. Recommendations to encourage sustainable utilization are suggested with the aim to reduce the threat of extinctions while maintaining community incomes. Keywords: Ecological vulnerabilities, socio-economic vulnerabilities, steroidal saponins, trade, vulnerability 17

18 7. Impact of Environmental Factors on the Growth of Plants Indu Tiwari Department of Botany, Government P. G. College, New Tehri, Uttarakhand Plant growth and distribution are limited by the environment. If any one of the environmental factors is less than ideal it will become a limiting factor in plant growth. Limiting factors are also responsible for the geography of plant distribution. For example, only plants adapted to limited amounts of water can live in deserts. Most plant problems are caused by environmental stress, either directly or indirectly. Therefore, it is important to understand the environmental aspects that affect plant growth. Environmental factors viz., light, temperature, water, and soil, greatly influence plant growth and geographic distribution. These factors determine the suitability of a plant for a particular location, management practices, and levels of inputs needed. A plant performs best and is least costly to propagate if it is grown under the most favorable environmental conditions. To maximize the production of any plant, it is important to understand how these environmental factors affect plant growth and development. Keywords: Limiting factors, environmental stress, management practices, geographic distribution, propagate 18

19 8. Observation on Status of Insect Pollinators in Binsar Wildlife Sanctuary, Western Himalayas, Uttarakhand, India Prachi Tamta, Manoj K. Arya and Dayakrishna Department of Zoology, D.S.B. Campus, Kumaun University, Uttarakhand, India Present investigations were undertaken to know the status of insect pollinators in Binsar Wildlife Sanctuary (BWLS), Kumaun Himalayas, Uttarakhand, India. The geographical location of BWLS is 29 o o 44 N and 79 o o 49 E and the altitude varies between 1200 to 2500 meters above sea level. BWLS represents the characteristic floral elements of Moist Temperate Forest surrounded by Chir pine and agricultural land. Insect as a pollinator has important role to maintain biodiversity in natural ecosystem. Insects including honey bees, bumble bees, butterflies, moths and beetles are vital for the pollination of many cultivated and wild plants. A total of 53 species of insects were found to facilitate the pollination process in the BWLS during the study period The identified pollinator species of insects belonged to order Lepidoptera (33 species), Hymenoptera (11 species), Diptera (five species) and Coleoptera (four species), respectively. Among the four recoded group of insects, Lepidopteran insects belonging to families Nymphalidae, Pieridae, Papilionidae, Lycaenidae, Danaidae, Satyridae, Acraeidae, Arctiidae, Noctuidae and Sphingidae were recorded in great numbers indicating efficient pollination in the study area. Similarly, Hymenopteran insects belonging to families Apidae, Scoliidae, Pompilidae, Vespidae and Xylocopidae were recorded in great numbers indicating efficient pollination in the study area. Among the Hymenopteran pollinator insect species, bees (Anthophora confusa Smith and Apis laboriosa Smith), bumble bees (Bombus sp. and Bremus sp.), and Xylocopa fenestrata Fabricius were recorded as the most frequent flower visiting and efficient pollinator insect species during the field visits in BWLS. Key words: Hymenopteran, insects, lepidopteran, pollinators, species 19

20 9. QSAR Based Virtual Screening of Novel Phytochemical Inhibitors Against HER2 in Breast Cancer Mahesha Nand 1, Priyanka Maiti 2, Subhash Chandra 2 * and Veena Pande 1 1 Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal, Uttarakhand, India. 2 Department of Botany, Kumaun University, S.S.J Campus, Almora, Uttarakhand, India. subhashku@kunainital.ac.in Breast cancer is the second most widespread cancer in the world among women with an estimated 1.67 million new cancer cases diagnosed in 2012 (25% of all cancers). It is the most frequent cancer in women both in more and less developed regions with slightly more cases in less developed (883,000 cases) than in more developed (794,000) regions. In Indian yearly incidence of breast cancer were approximately 1, 44,000. In Uttarakhand around 25% patients were diagnosed with breast cancer.her2 (human epidermal growth factor receptor 2) receptor is a membrane tyrosine kinase and when activated affects cell proliferation and survival. HER2 amplification is the primary pathway of HER2 receptor overexpression and is a major driver of tumor development and progression in a subset of breast cancers. HER2 amplification is found in case of 15% to 20% breast cancer. Therefore overexpressed HER2 receptor is an importantdrug target and the present investigation aims to explore some novel phytochemicals against HER2 by applying QSAR based virtual screening methods. Screened phytochemicals and their family are includes Cisplatin from Zanthoxylumarmatum, Homoharringtonine from Ulmusparvifolia, Daidzein from Puerariamirifica, Genistein from Puerarialobata and Catechins from Camellia sinensis. Screening anti-cancer compounds from several medicinal plants and natural compounds is attractive because they are safe, nontoxic and cost effective. The results of the work have identified several novel anti-cancer phytochemicals against breast cancer with future avenues in drug development against breast cancer. Keywords: Breast cancer, phytochemicals, machine learning, her2, breast cancer, text mining. 20

21 10. Biochemical Aspects of Photosynthesis and Respiration: An Implication to Environment and Social Awareness Preeti Bhatt and Chandra Kala Pant Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India Biochemical reactions in living systems regulate carbon dioxide (CO 2 ) and oxygen (O 2 ) balancein the atmosphere. Photosynthetic processes are highly significant in minimizing CO 2 level while the glycolytic pathways along with electron transport, producing CO 2 and water (H 2 O) at the expense of O 2, through respiration are widely known. In this presentation the biochemical aspects of respiration and photosynthesis has been considered to understand the consequences of these metabolic processes. Keeping in view the hazardous effects of deforestation and diseases caused by excessive intake of food materials (carbohydrates, proteins, fatty acids etc.) and alcoholism are discussed and their correlation with the environment and social awareness has been manifested. Keywords: Photosynthesis, respiration, environment, deforestation, health awareness 21

22 11. Analysis of Forest Vegetation, Population Structure and Regeneration Pattern in Kumaun Himalaya Meenakshi Negi and R. S. Rawal G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi- Katarmal, Almora , Uttarakhand, India The noticeable decline in natural regeneration is of great concern for west Himalayan forests due to several reasons, like, excessive lopping, over grazing and tree felling, non-viable seeds, extreme weevil and pest infestation, animal and bird predation resulting in low seed production. A study was therefore, carried out with an objective to quantitatively analyse representative forests around Nainital to record patterns of natural regeneration. Considering the dominance, a total of six forest types, P. roxburghii, mixed oak, Q. leucotrichophora, Q. lanuginosa, Q. floribunda and Q. semecarpifolia were identified. The tree density of these forests ranged from ind/ha, sapling density from ind/ha and total tree basal cover from m 2 /ha. Seedling density for both the years of observation was found maximum in Q. floribunda forest in rainy season i.e ind/ha (I year) and 3491 ind/ha (II year). Surprisingly, no seedlings were recorded from Q. semecarpifolia forest. The composition of tree and shrub layers differed markedly among all the forests. Q. floribunda forest supported the largest shrub population, while Q. semecarpifolia was poorest in this regard. With an exception of Q. semecarpifolia, the dominant species in all the forests had a stable population indicating good regeneration. The paper attempts to describe future possibilities of management in these forests. Keywords: Kumaun Himalaya, regeneration oak forests, phytosociology, population structure, 22

23 12. A Seasonal (Rainy Season) Survey and Study on the Availability of Different Fodder Species of Kumaun and Garhwal Region of Uttarakhand Hemlata Rathore, Netrapal Sharma and Satpal Singh Bisht Department of Zoology, D. S. B. Campus, Kumaun University, Nainital hem.rathore09@gmail.com Uttarakhand is a state which consists of 65% of forest cover and 86% region is mountainous It is surrounded by Himalaya from the northern side which provides a remarkable range of variation among the species the area consist of alpine zone and tropical forest. Trees leaves, shrubs, herbs, legumes and grasses are used as a fodder by the local villagers. Despite being a biodiversity rich state it is observed that there is an acute deficiency of quality fodder for the livestock. In order to check the availability of fodder species and their nutrient value a seasonal survey was done across the Kumaun and Garhwal region of Uttarakhand and also their availability was observed along different elevation. The survey was done approx in every district of Kumaun and Garhwal region.the fodder species collection is 480 and 390 in Kumaun and Garhwal region respectively. Dudhila (Ficus nemoralis), Eleusine indica,bhimal (Grewia optiva), Sakina (Indigofera), Timil (ficus roxburghii),tusharu (Debregeasia spp.) Doob (cynodon dactylon) are found to be most common according to availability during rainy season. The survey also highlighted the fact that there is lack of awareness among the villagers regarding the quality fodder and its management. The survey can help in identifying the fodder species rich area and also provide options for better fodder management techniques. Further awareness of quality fodder among villagers can slightly improves the deficit of fodder for the livestock. Keywords: Availability, deficit, Fodder, livestock 23

24 13. Allelopathic Effect of Bryophytes on Germination and Growth of Bidens Biternata (Lour.) Merr & Shreff Prabha B. Dhondiyal, Neerja Pande and Kiran Bargali Department of Botany, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India The present work embodies the allelopathic effect of bryophytes (Lunularia cruciata, Marchantia polymorpha and Plagiochasma appendiculatum) on the germination behaviour and biomass of Bidens biternata. The aim of the work was to study the effect of bryophyte species on unsoaked and presoaked seeds of B. biternata. The extract of these bryophytes were prepared in different organic solvents of varied polarity (acetone, ethanol and water) at two concentrations (100% & 50%). For pot experiments bryophytes were mixed with soil and as layer on the top of the soil. In in vitro and pot experiment the bryophytes had different degree of inhibitory effect on the germination and biomass of B. biternata as compared to control. The germination was completely inhibited in unsoaked seeds of Bidens in acetone and ethanol extracts of bryophytes at both the concentrations. In presoaked seeds of Bidens the germination was completely checked at higher concentration of acetone and ethanol extracts. The initiation of germination was also delayed in bryophytic extracts. The radicle length, plumule length, total seedling size and biomass varied between bryophyte species, solvent and concentration of the solvent used. Keywords: Allelopathy, bryophyte, Bidens, germination, inhibition, weed 24

25 14. Genetic Diversity among Sea Buckthorn (Hippophae Rhamnoides (L)) Using Molecular Marker Based Analysis Netrapal Sharma 1, Satpal Singh Bisht 1, Hemlata Rathore 1 and Sanjay Gupta 2 1 Department of zoology, D.S.B. Campus, Kumaun University, Nainital SBS PG Institute, Balawala, Dehradun, Uttarakhand np_biotech53@rediffmail.com The genetic diversity among thirty six genotypes of Hippophae Salicifolia D. Don of Uttarakhand region and Hippophae rhamnoides subsp. Turkestanica of Ladakh region were studied; using simple sequence repeats DNA (SSRs)-PCR, Random amplified primers (RAPD) and Cytochrome P450 analysis. The protein profiling and antioxidant profiling (quantitative and qualitative) of seeds were also done to select the superior genotypes having high economic importance. A total of seven SSR, twenty one RAPD and five CytoP450 primers were used for detecting genetic variability in Sea buckthorn seeds out of which seven SSR, eleven RAPD and five CytoP450 primers were highly reproducible and found suitable for use in PCR (Polymerase Chain Reaction) analysis. SSRs profile obtained by primer UTR- 15 was containing total of 8 bands ( kb), among these eight bands, one was found to be polymorphic (12.5%). The similarity coefficients based on SSR markers ranged from to with an average value of The polymorphism information content (PIC) value ranged from (UTR-015) to (UTR-12) with a mean of In RAPD, a total of 198 bands were scored corresponding to an average of 10.8 bands per primer with 138 bands showing polymorphism (73.2 %) with similarity coefficient ranging from to A dendrogram constructed based on the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering method revealed two major clusters of Uttarakhand (with sub-clusters) and Ladakh region (no sub-clusters). This study may useful in the selection of superior genotypes having higher nutraceutical value by using developed molecular and protein based markers in Uttarakhand and Ladakh regions. Key Words: Elaeagnaceae, Hippophae salicifolia, genotypes, sea buckthorn, genetic diversity 25

26 15. Impact of Lichen Litter on Soil Nutrients in the Temperate Areas of Kumaun Himalaya (Uttarakhand) Balwant Kumar and Mahesh Kumar Department of Botany, Kumaun University, S.S.J. Campus Almora, Uttarakhand, India Fresh lichen litter- Usnea, Everniastrum and Parmotrema were removed from Quercus twigs situated in temperate regions of Kumaun Himalaya. Agricultural soil samples were also collected for their chemical analysis and lichen decomposition activity. Individuals of selected lichens were mixed separately with this soil. Total fifteen sample buckets were used for lichen decomposition purpose. After six months of decomposition, observations were taken. Usnea was found to be the fastest decomposed lichen (about 91%) and increased the value of soil macronutrients- ph, N, P & K. It is the first attempt regarding impact of lichen decomposition on soil nutrients in the area. Key words: Kumaun Himalaya, lichen litter, decomposition, Usnea, soil nutrients 26

27 16. Adsorptive Removal of Zinc (II) by Activated Bark of Pinus Roxburghii : An Ecofriendly Approach Mahesh Chandra Arya, Shah Raj Ali and Rajesh Kumar Chemistry Department, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand , India mcarya181@gmail.com This work highlights an inexpensive, sustainable and ecofriendly adsorbent for the removal of Zinc (II) from its aqueous solution using activated bark powder of Pinus roxburghii as an adsorbent. Pine bark was collected from local Pinus roxburghii trees, also known as chir pine. The washed bark was oven-dried, ground and sieved through the mesh of different sizes ranging from 100µm to 150 µm. The powder was treated with HCl, NaOH and water till it got stabilised in aqueous medium. It was activated by heating it at C for suitable time and used as adsorbent to remove Zinc (II) from its aqueous solution. The adsorptive removal was studied in the concentration range 50 to150 mgl -1.The various parameters studied were effect of contact time between adsorbate and adsorbent, ph, concentration of adsorbate, size of particles of adsorbent, amount of adsorbent and adsorption isotherm. The activated bark powder of Pinus roxburghii showed maximum efficiency of 88% at optimum ph of 4.0 and contact time of 300 min for an initial concentration of 100 mgl -1. The data obtained was found to follow Langmuir adsorption isotherm model. The work demonstrates the removal of Zinc (II) from its aqueous solution efficiently using pine bark powder as an adsorbent. Keywords: Zinc (II), adsorption, pine bark 27

28 17. Observation on Environmental Effect on the Breeding Dynamics of Schizothorax Richardsonii (Gray), from Kumaun Himalayas, Uttarakhand, India. Uzma Siddiqui and H. C. S. Bisht Department of Zoology, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India Studies of environmental factors of an aquatic ecosystem are basic for understanding its breeding pattern. In aquatic system, the successful reproduction is based on sensing the environmental factors. Each factor plays an individual role but it is the synergistic effect of various environmental parameters which determines its breeding dynamics. Due to environmental factor such as temperature the breeding pattern is change in comparison to earlier period. ph is also increased due to forest fire and heavily construction of houses and roads. In Kumaun region of Uttarakhand. Dissolved Oxygen and free Carbon dioxide is also effected which affects the breeding biology of snow trout, Schizothorax richardsonii. Dissolved Oxygen is decreased during summer due to smog and smoke of forest fire. It is very surprising observation that the Nainital Lake had very rich population of Schizothorax richardsonii in the earlier but in present scenario there is no Schizothrax richardsonii at all in Nainital Lake except the exotic carp are growing. The breeding dynamics of this species depends on these environmental factors. Through the management practices for fish culture snow trout can be a successful breeder of Kumaun river by utilising scientific tools for fisheries. Key words: Breeding, dynamics, environmental parameters, reproduction, temperature 28

29 18. Antimalarial Activities of Some Plant Species Found in Kumaun Region Uttarakhand Pushpa Bhakuni Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand Malaria is a vector borne disease prevalent in tropical and developing countries causing significant morbidity and mortality. According to the latest estimates of WHO, in 2015 there were about 214 million cases of malaria worldwide and an estimated deaths attributable to it. Plasmodium falciparum (Pf), the most deadly malaria causing organism, has developed resistance to most commonly used drugs classes viz. the cinchona alkaloids, 4-aminoquinolines and the sulphonamides+diaminopyrimidines. After widespread resistance to commonly used antimalarial drugs viz. Quinine, Chloroquine and sulphadoxine+pyrimethamine, the Artemisinin derivatives became the only hope to fight against Malaria. However, alarming signals of Artemisinin resistance emanating from areas adjoining Cambodia are causing fears of rapid spread of Artemisinin resistance, leaving humanity to suffer this dreaded disease without a vaccine or effective and affordable antimalarial drugs. Therefore, there is an urgent need to search for novel antimalarials on a war footing. Plants have been used in the traditional medicine for the treatment of malarial fever and have indeed provided us two of the most powerful antimalarial drugs viz, Quinine (from cinchona bark) and Artemisinin (from Artemisia annua). Kumaun region in Uttarakhand state in the Indian Himalayan region is geographically and climatically diverse with number of plant species known for their medicinal properties. A Survey of ethnobotanical literatures reveal use of many locally found plant parts as traditional remedies. The treatment of various ailments including malarial fever. Such plant species are Achyranthes aspera, Ajuga bracteosa or parviflora, Berberis aristata or asiatica, Nepeta leucophylla, Pongamia pinnata, Roylea cinerea, Sida spinosa and Vitex nigundo found in Kumaun region. Therefore there is an urgent need of evaluation of biologically active compound against malaria from all these plant species which may be an alternative and effective antimalarial agent. This may help to decrease the burden of the disease and prevent emergence of resistance to currently used antimalarials. 29

30 19. A Review of Chemical Analyses and Ethno-medicinal Uses of Zingiberaceae Family Sapna, Pushpa Joshi and Manoj Dhuni Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India. sapna.chemistry91@gmail.com Zingiberaceae family is an important natural resource for food, perfumes, dyes, medicines, spices and aesthetics. India is one of richest and diverse region for Zingiberaceae having 22 genera and about 170 species, where North-east region is a zone of greatest concentration having about 19 genera and about 88 species. Natural products from plants of this family find application in several therapeutic formulations. These compounds belong to different chemical classes (alkaloids, terpenoids etc.) and have chemically diverse and complex structures. The rhizomes of plants belonging to this family are aromatic, tonic and stimulant. They are rich sources of essential oils which exhibit various physiological activities, antimicrobial, anti-arthritic, antioxidant, anticancer, anti-inflammatory, anti-diabetic, anti- HIV, neuro-protective etc. The objective of this paper is to make a collection and summarize the researches conducted in Zingiberaceae family so that researcher and academician may have better understanding about variations in chemical properties due to different region, climate and geographical conditions. The second objective of this paper is to identify the gap in the researches and to bridge the gaps. Keywords: Zingiberaceae, rhizomes, natural products, physiological activities 30

31 20. Effect of Zinc on Growth and Herbage Yield of Ocimum Gratissimum L. Mamta Bisht and Geeta Tewari Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital The genus Ocimum, (family Lamiaceae) is a rich source of aroma chemicals that find varied application in the food, flavor and pharmaceutical industry. Zinc is an essential micronutrient for the growth and reproduction of plants and active site of various enzymes that are responsible for driving many metabolic reactions in all crops. Growth and development would stop if specific enzymes were not present in plant tissue. Protein, and chlorophyll formation is significantly reduced in zinc-deficient plants. Therefore, a constant and continuous supply of zinc is needed for optimum growth and maximum yield. The purpose of this study is to determine the effect of zinc on growth and yield of Ocimum gratissimum L. A polyhouse study was conducted to determine the effect of zinc amendment on the growth and yield of Ocimum gratissimum L.The treatments of zinc were 600, 1000 and 1200 mg kg -1 in triplicate along with un-amended control.on increasing amendments there is a significant decrease in growth ( height and weight) of the plant, while yield increases significantly with unamended control. From the above study, it is suggested that this Ocimum species which finds wide application in herbal products, can be cultivated in zinc contaminated soil for its volatile constituents. Keywords: Ocimum gratissimum L., growth, oil yield, zinc 31

32 21. Chemotypic Variations in Ageratum Conyzoides L. Collected From Indo-Gangetic Plains Bhanu Kumar 1, Ankita Misra 1, Sharad Srivastava 1, Yashwant Singh Rawat 2 and Ajay Kumar Singh Rawat 1 1 Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, bhanu.kumar85@gmail.com Lucknow (Uttar Pradesh) 2 Botany Department, Kumaun University, Nainital (Uttarakhand) The present study investigates the chemotypic variations in germplasms of Ageratum conyzoides L., collected from Indo Gangetic Plains. Twenty-one germplasms were evaluated for morpho-anatomic variations and chemoprofiling through HPTLC. The morphological and anatomical variations were slight; however, quantification of biologically active marker compounds Precocene I (PI) and Precocene II (PII) using Toluene-Ethyl Acetate (9.8: 0.2) as solvent system revealed significant variation among germplasms. Precocene I and Precocene II content ranges from to 0.082% and to 0.078% respectively on dry weight basis. Cluster analysis based on the correlation matrix of metabolites, bifurcate the germplasms into two groups, separating NAC-13 with a considerable amount of both Precocene I and Precocene II. It is noteworthy that a negative correlation (Karl Pearson coefficient = ) was observed between the two marker compounds. The results clearly depict the variations in metabolite content among the intraspecific germplasms. Five elite germplasms were identified which can be utilised for commercial prospection for the development of herbal formulation with the desired effect in near future. Keywords: Ageratum conyzoides L., HPTLC, Indo-Gangetic plain, Precocene-I, Precocene- II 32