INTERNATIONAL SEED TESTING ASSOCIATION (ISTA) Zurich 2009

Transcription

1 Vigour Committee Report Zurich 2009

2 Vigour Committee Members: 2009 Resignations: John Hampton, NZ; Anders Lomholt, DK Chair Dr Alison A Powell GB United Kingdom Vice Chair Dr Michael Kruse DE - Germany Ms Patricia Brownfield US United States Dr Emanuela Casarini IT - Italy Dr Malavika Dadlani IN - India Dr Ibrahim Demir T - Turkey Ms Rasha El-Khadem AU - Austria Dr Hulya Ilbi T - Turkey Dr Mohammad Khajeh Hosseini IR - Iran Prof Seok Hyeon Kim K South Korea Dr Tim Loeffler NL - Netherlands Ms Gillian McLaren GB United Kingdom Dr Stan Matthews GB United Kingdom Ms Marie-Helene Wagner FR - France Ms Yan Rong Wang CH - China

3 Outline Method Development Development of new vigour tests Addition of new species to validated tests Research developments Method Validation Proficiency i tests Training The future

4 Method Development 1. Development of new vigour tests Rate of germination test: WG led by S Matthews 2. Addition of new species to validated tests Conductivity i test: soyabean 3. Research developments

5 1. Development of new vigour tests: Rate of Germination Test Work on maize Two publications (Matthews and Khajeh Hosseini, 2007, 2008; SST 34, 35): Rate (MGT) and soil emergence One publication (Khajeh Hosseini et al, 2009; SST 37); poster at Brazilian Congress: Field emergence and repeatability 13 o C, 3 labs (Seed Symposium, Brazil): Repeatability in 4 labs (13 and 20 o C); early counts and MGT Related to cold test and compared to AA (paper in preparation) 2007: Argentinian WG (AM, Bologna): repeatability in 8-13 labs (13 and 20 o C) Early counts and MGT related to field emergence : Validation study - repeatability in 6 labs (13 and 20 o C) : Validation study repeatability in 6 labs (13 and 20 C) Early counts related to field emergence

6 Validation study Leaders: Stan Matthews (UK), Rasha El Khadem (AT) 6 participants: Italy (LaRAS) France (SNES) Austria (2 labs, Pioneer; AGES) Iran (University of Ferdowsi) UK (University it of Aberdeen) 9 lots screened Feb/ March 2007 (Iran, UK); 6 lots selected for comparative test; distributed April 08 Assessment of rate of germination 13 o C; 20 o C; 2 runs at each temperature

7 Table: Comparison of mean physiological i l germination (%) after 66 h at 20 o C. Most rapid germination, high vigour (ranked 1); slowest germination (ranked 6). Seed lot Laboratory Overall mean Run Run Run Run Run Run = = = 2 2 2= 1= = =

8 Other Crops: Pepper: Demir et al l(2008) (2008); Seed Science and Technology, 36 Cucurbits: Mavi, Demir and Matthews, 2009; submitted for publication Cotton: with Cotton Seed Distributors in Australia

9 Cotton in Australia y = x R 2 = 0.83 *** 80 y = x R 2 = 0.76 *** % Filed d emergence Slow Older seed % Filed emergence Slow Older seed MGT (18 o C) d % Germination 3d (18 o C) Slow germinating lots give low field emergence Lab tests: Aberdeen, UK Field emergence: Australia

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11 2. Addition of new species to validated tests Conductivity it test: t soyabean Leaders: Rasha El Khadem (AT), Alison Powell (UK) 5 seed lots from Italy, sent out May laboratories: UK (2 labs: OSTS, Cambridge and Edinburgh) Italy (LaRAS) France (SNES) Iran (University of Ferdowsi) Turkey (Ege University) Netherlands (Seminis)

12 Comparison of conductivity readings from 7 laboratories Red numbers indicate seed lot ranking from 1 (high vigour) to 5 (low vigour) Lab Lot Lab A B C D E means (3) 17.4 (2) 15.3 (1) 21.2 (4) 22.2 (5) 19.0 d (3) 17.8 (2) 16.4 (1) 21.8 (4) 22.7 (5) 19.4 cd (2) 19.4 (3) 17.1 (1) 21.5 (4) 23.0 (5) 19.9 bc (2) 20.8 (3) 16.3 (1) 21.4 (4) 25.1 (5) 20.5 ab (1=) 18.0 (3) 15.8 (1=) 20.6 (4) 23.9 (5) 18.8 d (2=) 19.3 (2=) 17.4 (1) 22.8 (4) 26.3 (5) 21.0 a (2) 16.8 (3) 15.3 (1) 20.2 (4) 20.9 (5) 17.8 e Lot 18.0 c 18.5 c d 21.4 b 23.5 a means (2) (3) (1) (4) (5)

13 Validation of conductivity test for soyabeans: Method validation report submitted Following acceptance of report: Rules proposal to be submitted November 2009

14 3. Research developments Conductivity (EC) measurements of solute leakage from Brassica species Mirdad and Powell (2006); Seed Science and Technology, 34: EC predicts germination in artificially aged cauliflower and cabbage Demir et al (2008); Seed Science and Technology, commercial cabbage seed lots (2 cvs): EC predicts standard germination and vigour (germination after CD) Hampton et al (2009); Seed Science and Technology, 37: 26 Brassica seed lots: 3 vegetable species (18 lots), 3 forage species(8 lots) EC correlated with field emergence for all 26 lots and for B. napus (3 forage + 7 vegetable lots) Matthews et al (2009) submitted 12 commercial cabbage lots (1 cv): EC before and after CD Regression equations between EC before and after CD estimate emergence in modules

15 Conductivity alone may identify vigour differences Conductivity may replace germination after CD to predict vigour Conclusion Potential for vigour tests for Brassica spp based on conductivity. Next step- international comparative tests

16 Method Validation Controlled Deterioration test for Brassica species Method validation report accepted November 2008 Rules proposal at Annual Meeting 2009 Extension of the Conductivity test to Phaseolus spp Method Validation report accepted October 2008 Rules proposal at Annual Meeting 2009

17 Proficiency Test October 2008: Conductivity test for Pisum sativum 33 laboratories: 13 accredited for vigour Test preparation: Gillian McLaren, Edinburgh Thanks to Zita Ripka for supplying seed lots

18 Distribution of Z-Scores all lots all labs Vigour 08-3 P.sat Frequency More Z-Scores

19 Laboratory Ratings A B C BMP Accredited for vigour Vl Voluntary Voluntary, Accredited Voluntary, Non-accredited

20 Training Seed Moisture, Germination and Vigour Workshop Nakuru, Kenya, November 2008 Gillian McLaren vigour presentations

21 The Future Next year: Rules proposal: Addition of soyabean to species for conductivity test Validation and Rules proposal: Rate of Germination test for maize And beyond: Further work on use of conductivity test for Brassica Direct measurements To replace germination after CD Rate of germination test for other species: comparative tests