ISTA VIGOUR COMMITTEE REPORT

Transcription

1 ISTA VIGOUR COMMITTEE REPORT Alison A Powell University of Aberdeen, UK

2 Brief summary of some points in the report for 2010 Update on special projects and plans for

3 Rules development Introduction of new method Controlled deterioration test for Brassica species: introduced 2010 Introduction of new species Conductivity test Phaseolus vulgaris: Introduced to Rules 2010 Glycine max: Rules proposal accepted 2010; Introduced to Rules 2011 Introduction of Rules changes Radicle emergence vigour test for maize: Rules proposal 2011

4 Publications 4 papers in peer reviewed journals (SST, Italian Journal of Agronomy) 5 abstracts of oral papers at ISTA Seed Symposium abstracts of poster papers at ISTA Seed Symposium abstract, 5 th Int Food Legume Research Conference, Antalya, Turkey 3 articles, Argentinian publications

5 Update on special projects and plans for Controlled deterioration test: Approaches to reducing the test duration Marie-Hélène Wagner (SNES-GEVES, FR) Ibrahim Demir (University of Ankara, TR)

6 Marie-Hélène Wagner: Oil seed rape Adjustment of seed MC

7 Normal germination (%) CD test results similar using filter paper or roller method A1 A5 A6 B1 D1 D4 F2 H1 H2 H3 H4 J O P R CD 24h45 C at 20% MC by adding lots water CD 24h45 C at 20% MC by imbibition

8 Normal germination (%) Marie-Hélène Wagner: OSR: Replace germination test by conductivity measurement y = x R² = ** Conductivity (µs.cm -1.g -1 DW)

9 Ibrahim Demir: Short CD test for onion (5d vs 14 d) Reduce time for equilibration to 8h; Ageing for 18h; Germination count (radicle emergence) at 74 or 96h Relationship to field emergence and storage potential

10 Field emergence (%) Mean emergence time (d) Seed vigour tests Sowing 1 Sowing 2 Sowing 1 Sowing 2 CD /N 0.92** 0.91** 0.87** 0.76* CD /T 0.91** 0.91** 0.90** 0.77* Short CD/74h 0.93** 0.85* 0.97*** 0.75* Short CD/89h 0.89** 0.85* 0.88** 0.66 SG Also investigating: short CD for tomato, radish; replacing germination by EC

11 Vigour assessment Radicle emergence test: extension to other species Viola (Ibrahim Demir) Seedling emergence Mean emergence time (d) Storage for 12 months at: 5 o C 25 o C Standard germination 0.68 * 0.73 * MGT 0.96 *** 0.90 *** 0.94 *** 0.93 *** 2d germination 0.82 *** 0.82 ** 0.80 ** 0.80 ** MGT and 2d germination: Gazania, Salvia, Impatiens, Pelargonium

12 Radicle emergence test: Extension to other species Use of automated germination time courses: GEVES Species Temperature ( C) Samples analyzed Oilseed rape 10,20, Sunflower 12,20,25 18 Maize 15,20,30 32 Onion Cabbage Tomato Cucumber 18 7 Medicago truncatula (RIL pop) Alfalfa Pea 10, Wheat Use to identify best time for radicle emergence

13 Radicle emergence test: Extension to other species Collaborative tests on: Aubergine (Ibrahim Demir) Cotton (Stan Matthews) Onion (Hulya Ilbi) Oilseed rape (Gillian McLaren, Stan Matthews)

14 Comparative tests: Conductivity test: Also ongoing or planned: Cicer arietinum (chickpea) (Mohammad Khajeh Hosseini) Vigna unguiculata (cowpea) (Dionysia Fasoula) Accelerated ageing: cereals Research on: Biotinylated proteins Cell cycle and vigour Vigour tests for Elymus spp Canola and mustard (Marie-Helene Wagner) (Mohammad Khajeh Hosseini) (Yanrong Wang) (Bob Elliott) Influence of quality under different tillage practices; Effect of neonicotinoid treatments on vigour

15 Also ongoing or planned: Tetrazolium test for vigour: Glycine max Carina Gallo; Augusto Martinelli; Ignacio Aranciaga ; Jose França Neto Proficiency test for conductivity (2013) Gillian McLaren Workshop 2012 Canada

16 Thank you! ISTA Vigour Committee Alison Powell (Chair) GB Hulya Ilbi (Vice Chair) TR Emanuela Casarini IT Malavika Dadlani IN Ibrahim Demir TR Dionysia Fasoula CY Carina Gallo AT Bob Elliott CA Mohammad Khajeh Hosseini IR Tim Loeffler US Gillian McLaren UK Stan Matthews UK Marie-Hélène Wagner FR Yanrong Wang CN

17 Thank you for listening!