ADVANCING GENOMICS TO BETTER LIFE. Flask to field: Issues related to large scale cultivation. October 26 th 2016

Transcription

1 ADVANCING GENOMICS TO BETTER LIFE Flask to field: Issues related to large scale cultivation. October 26 th 2016

2 Outline: Brief introduction to Synthetic Genomics Inc.(SGI). Cultivation activities conducted at the Imperial Valley Facility (IVF). What are the issues related with large-scale algae cultivation? Contamination o Microbiological o Ash Water management Wrap up o Consumption o State regulations Final thoughts Conclusion 1

3 Positioned to build industries Innovation from the bench to the shelf Enabled by foundational core technology RESEARCH Revolutionizing science and medicine with next generation genomic solutions BIO-BASED PRODUCTION Reinventing cell-based production processes APPLIED PRODUCTS Create novel solutions and transform existing products Instrumentation / DNA printing Reagents DNA synthesis Bioinformatics Improves existing production hosts and develops entirely new synthetic production hosts New and more efficient routes to bioproducts Vaccines Gene/cell therapy Biologics Organ transplantation Antimicrobials Dietary supplements Algae protein Renewable chemicals Bio-based intermediates Plant enhancement Biofuels Food ingredients CORE TECHNOLOGY 2

4 Algal strain isolation and development capability The foundation of a wide range of innovative applied products Exploration and Discovery Biodiversity access to unique sites Large gene pathway database Extensive microbial collection including algae Leading genomic technologies Proprietary DNA synthesis Novel genomic tools Relevant for commercial applications Strain development Evolution & selection (non-gmo) Directed engineering (GMO) Commercial Targets Production system In-house lab scale fermentation Toll and partnered scale up fermentation N O T F O R D I S T R I B U T I O N 3

5 Algal Capability: Laboratory to commercial scale Characterization of phototrophic microalgae strains for biofuel production Laboratory Greenhouse Scale-down Pond simulation Programmable day light and temperature Photobioreactors Adaptive Evolution On-line monitoring Outdoor Commercial Scale Mini-ponds 100L PBR Outdoor Raceway Ponds 70 m2 1.9 m2 On-line monitoring NOT FOR DISTRIBUTION 4

6 Flask to field: Issues related to large scale cultivation. Introduction: Imperial Valley Facility (IVF) Large Scale Algae Cultivation Species Assessment Production Improvement Optimization Deployment COPYRIGHT 2016 SYNTHETIC G E N O M I C S, I N C. 5

7 Contamination: Issues to Consider. Types of contamination: Microbiological Ash COPYRIGHT 2016 SYNTHETIC G E N O M I C S, I N C. 6

8 Contamination: Microbiological Paraphysoderm sedebokerensis Chytrids are an infamous fungal contamination. They exhibit considerable variation in host ranges. Shown to infect both palmelloids (green cells) and the aplanospores (red cyst). Can result in catastrophic crop loss during red stage growth. 7

9 SGI algae capabilities Case Study: development of a commercially viable Haematococcus strain for the production of Astaxanthin Defeating Paraphysoderm sedebokerensis, an infectious pathogen of Haematococcus Pathogen Culturing and Characterization DIC Lipid stain Infection Models and Chemical Treatments Untreated Evolved Resistance Treated Next Gen Sequencing of Paraphysoderma sedebokerensis complete COPYRIGHT 2016 SYNTHETIC G E N O M I C S, I N C. 8

10 Flask to field: Issues related to large scale cultivation. Contamination Control: Ash Ash can constitute a wide range of harvested mass percentages. Ash content 8-60%+. Introduced into the system in various ways. o o Windblown debris. Chemical Precipitation. 9

11 Flask to field: Issues related to large scale cultivation. Dust and Debris

12 Flask to field: Issues related to large scale cultivation Chemical Precipitation: Well Water Analysis (ppm) Well 3 Well 5 Concentration (ppm) Well Water #5 Well Water # Analytes 11

13 Water Management: Issues to consider. What are some of the issues related to water management in large scale algae cultivation? Recharging evaporative losses. Evaporative pond management and control. o o Zero discharge facility? Blowdown management. o State paperwork and reporting requirements. Pumping expectations. o Enough capacity and capital to support the daily water and transfer schedules? Desired intake filtering of source water. o High costs of maintenance. o Potable water need: testing and paperwork Site sustainability due to water availability. Good Manufacturing Practices required? o Additional testing and analysis on inlet water required. 12

14 Water Management: Consumption during operation Evaporative loses: n Vary from site to site. n Can be unsustainable in some regions. Imperial Valley Evaporative Data: n Yearly Average: inches. n Added modifier of 20% for motility: inches/year. n Yearly evaporative consumption at 20 acres of 211 acre/ft total evaporative consumption. 13

15 Water Management: Site Sustainability. Site water rights vary from state to state. In the Imperial Valley the Imperial Valley Irrigation District (IID), controls water use based on site acreage. An 80 acre site has yearly rights to approximately 251 acre/ft of water per year. Evaporative Consumption at 20 acres. 211 Acre Ft/year. Approximated site start up for 20 acres. 17 acre ft/year Approximated blowdown consumption. Typically observed blow down of 5% of start up volume:.9 acre/ft. Total Site usage at 20 acres approximated at: 229 acre/ft/year. 80 acres of owned area = 251 acre feet of available production water per year. 251 acre feet of water can support approximately 20 acres of wet production area annually. 14

16 Conclusion: There are many more issues related with large scale cultivation. Cost of goods (COGs). The obvious unexpected costs. 15

17 Acknowledgements: Thank you! 16

18 Questions?

19 C O N F I D E N T I A L A N D N O T F O R D I S T R I B U T I O N

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21 Flask to field: Issues related to large scale cultivation. Issues: Water Management Contamination Control Labor COSTS 20

22 Flask to field: Issues related to large scale cultivation Other issues related to water management. 21

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28 C O N F I D E N T I A L A N D N O T F O R D I S T R I B U T I O N