CRYOPRESERVATION EQUIPMENT

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1 CRYOPRESERVATION EQUIPMENT SCIENCE & INDUSTRY Gases for Science, Leisure and Industry

2 preservation brochure3.qx6 16/8/07 14:58 Page 1 CRYOSERVICE - THE DIFFERENCE The quality of Chart MVE products, backed by CryoService expertise, provides the highest level of sample security supporting biomedical applications such as assisted reproduction, oncology research, immunology, gene therapy and banking of tissue, bone marrow, stem cells and cord blood. REDUCING LIFETIME COSTS THE LATEST TECHNOLOGY, THE GREATEST BENEFITS Chart MVE freezers have consistently low Liquid Nitrogen boil off rates as a result of maximum thermal performance. This is achieved through the use of an advanced chemical vacuum retention systems where palladium dioxide is placed in the vacuum cavity to absorb moisture which increases the life and performance of the vacuum. The benefit to you is low Liquid Nitrogen consumption which reduces the cost of storing your samples. HIGHEST SAMPLE SECURITY From our inception in 1970, we have embraced innovation and new technology to produce a World Class solution to the supply of specialist gases. Originally established to provide low-volume cryogenic deliveries to the scientific market, we quickly developed a reputation as the supplier of choice for critical applications, pioneering a unique delivery service and developing innovative storage solutions that provide tangible benefits to thousands of customers across the UK. We now supply an extensive range of cryogenic and specialist gases to a wide range of markets in the science, leisure and industrial sectors. This is complemented by comprehensive equipment options and in-depth application knowledge. Our unique Intelligent and Managed Gas concepts seamlessly combine innovation, service and new technology to provide the ultimate in industrial and technical gas solutions. We are the preferred UK distributor for Chart Biomedical who manufacture the World s leading range of products for cryopreservation of biological materials under the MVE brand. For more than thirty years, Chart MVE has led the industry setting the standard for cryopreservation technology. Our total dedication to service means that we are able to exceed the expectations of our customers time and time again by delivering: 7 Day Support To ensure that we are always available to offer support as and when you need it. National Network We have a network of engineers across the UK operating from 10 depots to provide you with local support and a quick response. Solutions to Meet Your Needs Over 30 years experience operating across a wide range of market segments means that we have a breadth of solutions that can be tailored to meet your needs. Liquid Nitrogen offers unparalleled security for the cryopreservation of biological samples. At Liquid Nitrogen temperatures there is a wider safety margin than via -150 C mechanical freezers and no reliance on a supply of electricity. The Chart MVE range have long Liquid Nitrogen holding times as a result of their superior vacuum performance, which is maintained over the life of the product, providing enhanced security for stored samples. The Chart MVE Eterne vapour phase freezer range have the longest holding times in the industry. EASE OF USE The Chart MVE range have been designed with ergonomic features to ensure samples can be removed easily and safely. An example of this is the rotating platform and fold up steps on the Eterne vapour freezers. LONG-TERM SAMPLE VIABILITY To ensure long-term viability, samples need to be stored at a constant range of temperatures below a minimum critical threshold, for living cells this is -130 C. The upper limit of the range should be well below this temperature i.e. at least lower than -140 C. Storing in liquid is not always practical as it poses health and safety and cross contamination issues. The alternative is to store in vapour, however not all vapour freezers are able to maintain a temperature lower than -140 C. This is not the case with Chart MVE products, as their design, excellent thermal insulation and vacuum performance, ensures your samples are consistently kept below the critical temperature. Chart MVE were the first company to develop freezers specifically designed for storage of samples in vapour phase. The Eterne vessels feature a smaller, offset lid which results in a greater surface area being protected by a vacuum insulated surface, this stops heat radiating into the vessel and ensures samples are maintained in a -190 C environment. This temperature can be maintained in excess of 20 days without additional filling. LONG PRODUCT LIFETIME There is a standard 2 year warranty on all equipment (parts and labour) and a 5 year vacuum warranty. Chart MVE s vacuum warranty surpasses industry standards due to the rugged design and high quality welds. Chart MVE aluminium products have only one weld compared to two welds on other freezer products supplied in the market. All these features result in a longer product lifetime which both protects your samples and reduces the frequency with which you have to replace freezers. TECHNICAL SUPPORT The Eterne and MVE ranges of stainless steel freezers can be supplied with a state-of-the-art TEC 3000 controller, which initiates automatic filling and constantly monitors and logs the temperature at two separate heights in the freezer. The data can be downloaded to a PC and alarms can be connected to a BMS system. CryoService have over 30 years experience of supplying and maintaining Liquid Nitrogen vessels. We have an extensive team of service engineers based around the country who carry the most common spare parts required by Chart MVE freezers. Telephone support is available 7 days a week including outside normal business hours. TOTAL SOLUTIONS CryoService have the experience and expertise to assist you in designing a complete Cryopreservation laboratory. This includes liquid gases and associated storage vessels, superinsulated vacuum transfer pipelines, the best Liquid Nitrogen vapour storage freezers on the market, oxygen depletion systems and business management outputs...a total package from one provider.

3 preservation brochure3.qx6 16/8/07 14:58 Page 1 CRYOSERVICE - THE DIFFERENCE The quality of Chart MVE products, backed by CryoService expertise, provides the highest level of sample security supporting biomedical applications such as assisted reproduction, oncology research, immunology, gene therapy and banking of tissue, bone marrow, stem cells and cord blood. REDUCING LIFETIME COSTS THE LATEST TECHNOLOGY, THE GREATEST BENEFITS Chart MVE freezers have consistently low Liquid Nitrogen boil off rates as a result of maximum thermal performance. This is achieved through the use of an advanced chemical vacuum retention systems where palladium dioxide is placed in the vacuum cavity to absorb moisture which increases the life and performance of the vacuum. The benefit to you is low Liquid Nitrogen consumption which reduces the cost of storing your samples. HIGHEST SAMPLE SECURITY From our inception in 1970, we have embraced innovation and new technology to produce a World Class solution to the supply of specialist gases. Originally established to provide low-volume cryogenic deliveries to the scientific market, we quickly developed a reputation as the supplier of choice for critical applications, pioneering a unique delivery service and developing innovative storage solutions that provide tangible benefits to thousands of customers across the UK. We now supply an extensive range of cryogenic and specialist gases to a wide range of markets in the science, leisure and industrial sectors. This is complemented by comprehensive equipment options and in-depth application knowledge. Our unique Intelligent and Managed Gas concepts seamlessly combine innovation, service and new technology to provide the ultimate in industrial and technical gas solutions. We are the preferred UK distributor for Chart Biomedical who manufacture the World s leading range of products for cryopreservation of biological materials under the MVE brand. For more than thirty years, Chart MVE has led the industry setting the standard for cryopreservation technology. Our total dedication to service means that we are able to exceed the expectations of our customers time and time again by delivering: 7 Day Support To ensure that we are always available to offer support as and when you need it. National Network We have a network of engineers across the UK operating from 10 depots to provide you with local support and a quick response. Solutions to Meet Your Needs Over 30 years experience operating across a wide range of market segments means that we have a breadth of solutions that can be tailored to meet your needs. Liquid Nitrogen offers unparalleled security for the cryopreservation of biological samples. At Liquid Nitrogen temperatures there is a wider safety margin than via -150 C mechanical freezers and no reliance on a supply of electricity. The Chart MVE range have long Liquid Nitrogen holding times as a result of their superior vacuum performance, which is maintained over the life of the product, providing enhanced security for stored samples. The Chart MVE Eterne vapour phase freezer range have the longest holding times in the industry. EASE OF USE The Chart MVE range have been designed with ergonomic features to ensure samples can be removed easily and safely. An example of this is the rotating platform and fold up steps on the Eterne vapour freezers. LONG-TERM SAMPLE VIABILITY To ensure long-term viability, samples need to be stored at a constant range of temperatures below a minimum critical threshold, for living cells this is -130 C. The upper limit of the range should be well below this temperature i.e. at least lower than -140 C. Storing in liquid is not always practical as it poses health and safety and cross contamination issues. The alternative is to store in vapour, however not all vapour freezers are able to maintain a temperature lower than -140 C. This is not the case with Chart MVE products, as their design, excellent thermal insulation and vacuum performance, ensures your samples are consistently kept below the critical temperature. Chart MVE were the first company to develop freezers specifically designed for storage of samples in vapour phase. The Eterne vessels feature a smaller, offset lid which results in a greater surface area being protected by a vacuum insulated surface, this stops heat radiating into the vessel and ensures samples are maintained in a -190 C environment. This temperature can be maintained in excess of 20 days without additional filling. LONG PRODUCT LIFETIME There is a standard 2 year warranty on all equipment (parts and labour) and a 5 year vacuum warranty. Chart MVE s vacuum warranty surpasses industry standards due to the rugged design and high quality welds. Chart MVE aluminium products have only one weld compared to two welds on other freezer products supplied in the market. All these features result in a longer product lifetime which both protects your samples and reduces the frequency with which you have to replace freezers. TECHNICAL SUPPORT The Eterne and MVE ranges of stainless steel freezers can be supplied with a state-of-the-art TEC 3000 controller, which initiates automatic filling and constantly monitors and logs the temperature at two separate heights in the freezer. The data can be downloaded to a PC and alarms can be connected to a BMS system. CryoService have over 30 years experience of supplying and maintaining Liquid Nitrogen vessels. We have an extensive team of service engineers based around the country who carry the most common spare parts required by Chart MVE freezers. Telephone support is available 7 days a week including outside normal business hours. TOTAL SOLUTIONS CryoService have the experience and expertise to assist you in designing a complete Cryopreservation laboratory. This includes liquid gases and associated storage vessels, superinsulated vacuum transfer pipelines, the best Liquid Nitrogen vapour storage freezers on the market, oxygen depletion systems and business management outputs...a total package from one provider.

4 preservation brochure3.qx6 16/8/07 14:58 Page 1 CRYOSERVICE - THE DIFFERENCE The quality of Chart MVE products, backed by CryoService expertise, provides the highest level of sample security supporting biomedical applications such as assisted reproduction, oncology research, immunology, gene therapy and banking of tissue, bone marrow, stem cells and cord blood. REDUCING LIFETIME COSTS THE LATEST TECHNOLOGY, THE GREATEST BENEFITS Chart MVE freezers have consistently low Liquid Nitrogen boil off rates as a result of maximum thermal performance. This is achieved through the use of an advanced chemical vacuum retention systems where palladium dioxide is placed in the vacuum cavity to absorb moisture which increases the life and performance of the vacuum. The benefit to you is low Liquid Nitrogen consumption which reduces the cost of storing your samples. HIGHEST SAMPLE SECURITY From our inception in 1970, we have embraced innovation and new technology to produce a World Class solution to the supply of specialist gases. Originally established to provide low-volume cryogenic deliveries to the scientific market, we quickly developed a reputation as the supplier of choice for critical applications, pioneering a unique delivery service and developing innovative storage solutions that provide tangible benefits to thousands of customers across the UK. We now supply an extensive range of cryogenic and specialist gases to a wide range of markets in the science, leisure and industrial sectors. This is complemented by comprehensive equipment options and in-depth application knowledge. Our unique Intelligent and Managed Gas concepts seamlessly combine innovation, service and new technology to provide the ultimate in industrial and technical gas solutions. We are the preferred UK distributor for Chart Biomedical who manufacture the World s leading range of products for cryopreservation of biological materials under the MVE brand. For more than thirty years, Chart MVE has led the industry setting the standard for cryopreservation technology. Our total dedication to service means that we are able to exceed the expectations of our customers time and time again by delivering: 7 Day Support To ensure that we are always available to offer support as and when you need it. National Network We have a network of engineers across the UK operating from 10 depots to provide you with local support and a quick response. Solutions to Meet Your Needs Over 30 years experience operating across a wide range of market segments means that we have a breadth of solutions that can be tailored to meet your needs. Liquid Nitrogen offers unparalleled security for the cryopreservation of biological samples. At Liquid Nitrogen temperatures there is a wider safety margin than via -150 C mechanical freezers and no reliance on a supply of electricity. The Chart MVE range have long Liquid Nitrogen holding times as a result of their superior vacuum performance, which is maintained over the life of the product, providing enhanced security for stored samples. The Chart MVE Eterne vapour phase freezer range have the longest holding times in the industry. EASE OF USE The Chart MVE range have been designed with ergonomic features to ensure samples can be removed easily and safely. An example of this is the rotating platform and fold up steps on the Eterne vapour freezers. LONG-TERM SAMPLE VIABILITY To ensure long-term viability, samples need to be stored at a constant range of temperatures below a minimum critical threshold, for living cells this is -130 C. The upper limit of the range should be well below this temperature i.e. at least lower than -140 C. Storing in liquid is not always practical as it poses health and safety and cross contamination issues. The alternative is to store in vapour, however not all vapour freezers are able to maintain a temperature lower than -140 C. This is not the case with Chart MVE products, as their design, excellent thermal insulation and vacuum performance, ensures your samples are consistently kept below the critical temperature. Chart MVE were the first company to develop freezers specifically designed for storage of samples in vapour phase. The Eterne vessels feature a smaller, offset lid which results in a greater surface area being protected by a vacuum insulated surface, this stops heat radiating into the vessel and ensures samples are maintained in a -190 C environment. This temperature can be maintained in excess of 20 days without additional filling. LONG PRODUCT LIFETIME There is a standard 2 year warranty on all equipment (parts and labour) and a 5 year vacuum warranty. Chart MVE s vacuum warranty surpasses industry standards due to the rugged design and high quality welds. Chart MVE aluminium products have only one weld compared to two welds on other freezer products supplied in the market. All these features result in a longer product lifetime which both protects your samples and reduces the frequency with which you have to replace freezers. TECHNICAL SUPPORT The Eterne and MVE ranges of stainless steel freezers can be supplied with a state-of-the-art TEC 3000 controller, which initiates automatic filling and constantly monitors and logs the temperature at two separate heights in the freezer. The data can be downloaded to a PC and alarms can be connected to a BMS system. CryoService have over 30 years experience of supplying and maintaining Liquid Nitrogen vessels. We have an extensive team of service engineers based around the country who carry the most common spare parts required by Chart MVE freezers. Telephone support is available 7 days a week including outside normal business hours. TOTAL SOLUTIONS CryoService have the experience and expertise to assist you in designing a complete Cryopreservation laboratory. This includes liquid gases and associated storage vessels, superinsulated vacuum transfer pipelines, the best Liquid Nitrogen vapour storage freezers on the market, oxygen depletion systems and business management outputs...a total package from one provider.

5 APPLICATION INFORMATION Product Range Description Vial Capacity Range Applications Eterne/HE Stainless Steel Freezer 15,600 to 79,950 Storage in vapour of stem cells, blood bags, vials or straws MVE Series Stainless Steel Liquid or Freezer 2,400 to 33,000 Storage in vapour or liquid of stem cells, blood bags, vials or straws CryoCart Portable Workbench N/A Workbench to maintain sample temperature when picking samples or transporting around the laboratory Cryosystem Aluminum Rack Liquid Freezer 750 to 6,000 Storage in liquid of vials in racks SC/XC Series Aluminum Canister Liquid Freezer <1,500 Storage in canisters of straws or vials Shippers Shipper for Samples <1,134 Shipment of samples by air and land with hold times from 8 to 85 days Lab Series Liquid Nitrogen Dewar N/A Dermatology treatments, supply to Controlled Rate Freezers and manual top-up of Liquid Nitrogen Freezers For more information on each product range see the individual data sheets. CryoService can also supply a range of freezer alarms, freezer monitoring systems and Oxygen Depletion Monitors to compliment this product range.

6 Warndon Business Park Worcester WR4 9RH United Kingdom Tel: +44 (0) Fax: +44 (0) /01