Ensure high performance chromatography by using particle free mobile phases and sample solutions

Transcription

1 Ensure high performance chromatography by using particle free mobile phases and sample solutions

2 Protect your columns, Particles from your samples and mobile phase can contaminate your system and/or block the frits of your HPLC column. By filtering your samples, you can ensure that your column s lifetime is not cut short by particle-induced blockages. Particulate matter is often abrasive, and may cause unnecessary wear on injection valves and switching valves. By membrane filtering of samples and mobile phases, you protect your instrument from particle-induced wear, and minimize unnecessary downtime. Additionally, if you use columns with small internal diameters, it s even more important to prevent blockage of your narrow-bore capillaries by membrane filtering blocked capillaries cost time and money. Choose the right membrane type Agilent Technologies offers a range of filters to remove particles down to 0.45 µm in diameter. To remove the smallest particles, filters are also available with a pore size of only 0.2 µm. Select the right membrane type to match your sample solvent: regenerated acetate nitrate PTFE Nylon

3 look after your instruments For samples containing coarse particles, there are glass fiber filters that can be used either on their own or as pre-filters in series with any other membrane filter. Or for quick, simple filtration, you can choose 2 in 1 filters combination filters that offer savings in both sample and time compared with multiple individual filters. Choose the right filter diameter The diameter determines the filter cross-sectional area of the filter surface. The same membranes are used in all filter diameters. 30 mm filters are designed for large sample volumes or for solvent filtration and offers increased speed over small diameter filters. 25 mm is the industry standard size and are conveniently and economically packaged in a 200/pack. 13 mm offer the best compromise between sample volume and holdup volume and are our most popular size for HPLC use. 3 mm are ideal for small samples (<1 ml) when minimum sample loss is desirable. Holdup volume: <7 µl.

4 safeguard your analytical results, Pre-filters The pre-filters have a 100% borosilicate glass fiber membrane that is chemically inert and resistant to most solvents, both organic and inorganic. The mechanically rigid fiber structure has a high surface area that provides outstanding particle retention capacity while maintaining low filter resistance. You can choose between the GF53, for coarse particles (down to 3 mm) and the GF92, for particles down to 1 mm. You can use these glass fiber filters either on their own, for single-step filtration, or in series with any other membrane filter.

5 and preserve your samples. Regenerated the universal membrane The regenerated filters, because of their low protein binding, give the best performance and highest sample recovery rates. If you are working with large sample volumes, the Recovery of PAH: Naphthalene, Acenaphthylene, Acenaphthene, Fluorene, Phenanthrene, Anthracene, Pyrene Low Protein Adsorption of CA and RC membranes 30 mm filter offers a speed increase of up to 35% over 25 mm diameter filters. And the low protein adsorption and low solvent extractables (organic and aqueous) mean that your sample retains its integrity.

6 2 in 1 filters A B For quick, simple filtration of difficult-tofilter solutions, you can choose one of the 2 in 1 filters. These combine a coarse glass fiber pre-filter with a nitrate or regenerated membrane in a single housing. Because the coarse particles are retained on the glass fiber pre-filter, and do not block the surface of the membrane, 2 in 1 filters require less force, and give a higher throughput. And the combination costs less than two individual filters. 2 in 1 filters are economical they save you time, sample and money. Agilent 2 in 1 filter with glass fibre pre-filter (A) and 0.45 µm membrane (B) a) b) Carrot juice (left) a) filtered with Agilent 2 in 1 filter b) filtered with conventional filter

7 Agilent Technologies filters are mounted in inert housings of either polypropylene or polycarbonate and are capable of sustaining a pressure of greater than 7 bar (105 psi). All filters are manufactured under ultra-clean conditions. Safe filtration The simple, disposable units have unique rimmed housings, which eliminate the need for glues or binders, thus eliminating the possibility of introducing artifacts during filtration. In exhaustive comparative tests, Agilent Technologies filters produced cleaner, purer filtrates than filters from other manufacturer. When filters were extracted with commonly-used solvents, only the Agilent Technologies filters produced clean filtrates with negligible Methanol extract measured with HPLC at 254 nm

8 Acetonitrile extract measured with HPLC at 254 nm extracted impurities. Filters from other manufacturers produced contaminated filtrates containing significant extracted impurities. When you filter your sample through an Agilent Technologies filter, you can be sure that the only change is that the particles are removed; nothing is added. Choose the right size for your sample volume Sample Hold up Effective Diameter volume volume filter area [mm] [ml] [µl] [cm 2 ] < < < < 1 <

9 The solvent filtration apparatus permits the rapid filtration of most HPLC solvents. Removing the particulates from the solvents ensures that the solvent delivery system and other parts subject to wear have maximum life expectancy. In addition, the removal of particles also minimizes the risk of clogging column inlet frits. Mobile Phase Clean-up An added benefit to the process of filtrating the mobile phase also degasses the mobile phase for those people who do not have an on-line degasser installed with their system. Degassing of the solvents also prevents the formation of spurious peaks within the detector due to solvent outgassing at the low pressure end of the chromatograph. The solvent filtration kit consists of everything you need to successfully filter your solvents a 250 ml glass reservoir, tapered funnel base with PTFE-coated sieve, tapered top, 1000 ml flask and clamp. The filter apparatus can be used with standard membrane filters 47 or 50 mm in diameter.

10 Ordering Information (All membrane filters in packs of 100 or 200) Pre-filters, (100/pk) Sample Diameter volume Agilent [mm] [ml] Membrane Housing Part No Glass fiber GF53 Polypropylene Glass fiber GF92 Polypropylene Membrane filters, (100/pk) Pore Sample Diameter size volume Agilent [mm] [µm] [ml] Membrane Housing Part No Cellulose Polycarbonate acetate Regenerated Polypropylene Regenerated Polypropylene PTFE Polypropylene PTFE Polypropylene Regenerated Polypropylene * Regenerated Polypropylene * Regenerated Polypropylene Regenerated Polypropylene PTFE Polypropylene PTFE Polypropylene * < 1 Nylon Nylon < 1 Nylon Nylon * filter with mini-tip. All other filters have male luer tip

11 Membrane Econofilters filters, (200/pk) Pore Sample Diameter size volume Agilent [mm] [µm] [ml] Membrane Housing Part No Regenerated Polypropylene Regenerated Polypropylene Nylon Polypropylene Nylon Polypropylene PTFE Polypropylene PTFE Polypropylene in 1 filters, (100/pk) Pore Sample Diameter size volume Agilent [mm] [µm] [ml] Membrane Housing Part No Glass fiber Polycarbonate Cellulose nitrate Glass fiber Polypropylene Regenerated Solvent Filtration Apparatus Description Agilent Part No. Solvent filtration kit (Consists of a 250 ml glass reservoir, tapered funnel base with PTFE coated sieve, tapered top, 1000 ml flask and clamp) Regenerated filter membranes 47 mm, pore size 0.45 µm, 100/pk Nylon filter membranes 47 mm, pore size 0.45 µm, 100/pk

12 To place an order or for additional information, visit our web site at click on Columns and Accessories and select Syringe Filters under LC Consumables. Or, contact your local Agilent sales office or authorized Agilent distributor. Copyright 2002 Agilent Technologies The information in this publication is subject to change without notice. All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under the copyright laws. Printed in Germany July 30, 2002 Publication No EN