ENVIRONMENTAL AND WATER QUALITY MONITORING PROGRAM

Transcription

1 ENVIRONMENTAL AND WATER QUALITY MONITORING PROGRAM Research Animal Resources Center University of Wisconsin Madison Overview and Training Program designed by Laurel Statz of the Comparative Pathology Lab

2 YOUR HAND OUTS R.A.R.C. SOP PATH # 25, ENVIRONMENTAL MONITORING R.A.R.C. SOP PATH # 31, AUTOCLAVE TESTING TOLERANCE DOCUMENT TECHNICAL ARTICLE COMPARING USE OF ATP WITH RODAC PLATES LIST OF PLAN FOR YOUR FACILITIES THE ANNUAL REPORT FOR YOUR OWN FACILITY SANITATION FLOW CHART QUALITY ASSURANCE BROCHURE

3 OVERVIEW OF CAGE WASH MONITORING The Charm NovaLUM system is the system used in our monitoring program. The product employs firefly enzyme, luciferin and luciferase in creating a firefly glow which is read by the handheld reader device This system monitors for the presence of ATP on surfaces. The presence of ATP indicates: that the surface has not been adequately cleaned or that ATP is present in water in our water quality program. Besides bacteria the system measures the presence of : Molds and yeasts Food or Organic product residues Biofilms

4 WHAT IS ATP? denosine ri hosphate is a universal energy source; a high energy compound is present in all living (or once living) organisms: Plants Animals Microorganisms occurs in Produc;on Environments as: free ATP soma;c ATP microbial ATP..Biofilms

5 WHY USE ATP? PocketSwab Plus ATP Technology is a Rapid, Accurate, Cost effec;ve method of Sanita;on Verifica;on Easy to Use and Interpret Numerical Results for documenta;on Real Time Results provide informa;on for immediate correc;ve ac;on steps prior to produc;on.

6 ATP TECHNICAL DOCUMENT Published in November 1998 Contemporary Topics Journal Compares the results of ATP methods with the old standard RODAC Plates Favorable results towards the use of ATP Available in your handouts in hard copy.

7 PRINCIPLE OF ATP TECHNOLOGY ATP + Luciferin + Luciferase + Buffering = Chemical Reaction In effect the firefly enzymes adhere to the ATP and create a glow, like a firefly that can be read and numerated by the handheld reader. The buffering agent and the tablets contained in the pocket swab make this possible.

8 WHY TEST FOR ATP? 1. Total indicator: clean vs. dirty - a cleaner and safer surface 2. Real time test (5 second Readout): allows for corrective action 3. Numeric result: documentation of test results 4. Reduce ATP: Reduce Microorganisms 5. Training/Education: empower sanitation crew For illustration purposes only

9 SUCCESS OF ATP PROGRAM DEPENDS ON: Determining swabbing loca;ons & test frequency Customizing instruments with sample plans Monitoring with ATP to iden;fy SOP Devia;ons Swabbing technique, number of sample points Re- clean, re- test, and possibly integrate with micro data. Technical support by vendor personnel.

10 Swa b H a nd le P o ck e t S w a b PATENTED TECHNOLOGY SHELF STABLE Write- on Label for Swab Sampling Site Patented Thread Design for C onsistent Results User to User Bod y Premoistened Swab with Biofilm Breaking Agent Metal Detectable Foil Seal C lear Liquid with Sanitizer Buffering Agent Microtub e 36 f-77 f C lear Microtube Allows Reaction O bservation Luciferin/Luciferase in Tablet Form for Uniform Dosage

11 OUR TWO PART ENVIRONMENTAL MONITORING PROGRAM Cage Wash System Check: Caging Equipment Stationary Objects Water Monitoring: Source Water, nonconditioned Conditioned water Wash Water Water given to animals. Water at the furthermost place from source

12 No More White Glove Test. REVIEW OF ENVIRONMENTAL MONITORING S.O.P. Spend some time reading the SOP R.A.R.C. SOP # Path 25 Also: Refer to the Cleaning flow chart handout.

13 REVIEW OF ENVIRONMENTAL MONITORING SOP. WHERE DO WE NEED ATTENTION? What is working well. Most people call and make sure the timing for swabs is good. The new calendar that gives facilities a specific month to run swabs, seems to be working out well. The new reports will give a clear idea how well your sanitation program works. I see fewer and fewer cases of swabs being pre exposed when I get them. This means I hardly ever get swabs with the foil membrane already broken.

14 REVIEW OF ENVIRONMENTAL MONITORING SOP WHERE WE NEED ATTENTION. Where can we improve? Consistency in following plans Consistency in testing at least 5 similar items from the same wash cycle. Writing the start time of swabbing onto the work sheet. There is confusion about testing water Taking care to use the swabs properly and carefully. Identifying the materials that an item is made of. (example: polycarbonate is not plastic)

15 SURFACES & TOLERANCES Non-Porous with a tolerance level of 1500 RLU Stainless Steel, Glass, Glazed Ceramic, Polycarbonate, Polysulfone and similar new age plastics, etc.. Porous with a tolerance level of 4500 RLU Rubber, Plastics, Cement, Wood, Unglazed ceramic, PVC, etc. Water Tolerance is always Zero RLU Ever Wonder where those tolerance numbers come from?

16 Tolerance Levels ü The limits of RLU directly relate to the failure levels of the RODAC Plates. ü 1500 RLU occurs where RODAC plates from nonporous items would fail ü 4500 RLU occurs where RODAC plates from porous items would fail. ü Water should be free of ATP

17 TYPICAL RODENT FACILITY PLANS INCLUDE Mouse caging Rat Caging Feeder Wires Water bottles Water Sippers Microisolator Lids Shelving Countertops Room Wall Water Larger facilities will have a hard time swabbing very much stationary equipment and may request extra swabs for this.

18 Plans: Items Cleaned in Automatic Washers such as Cage Washers, Tunnel Washers and Bottle Washers. Stationary equipment such as floors, walls,sinks, lixits. Water-both source water used for washing and water given to animals. Detergents and chemicals used in the washing process. (when suggested, not always) The SMPH has an independent water testing program. Water information does not pertain to them.

19 Water Testing Use the water test sheet for your water testing. Depending on the size of the facility (facilities washing more than 1000 cages weekly) you may receive extra swabs for water. Run the Hach Aquatest 5 Parameter test strip first and record on the worksheet. This product is available through Fisher on MDS (catalog # ) When you have tested with swabs send the worksheet with the Hach test results along with your swabs to RARC

20 A WATER PLAN INCLUDES Primary source of water into facility After any water treatments such as water softener, acidifier etc. Water farthest from the primary source. Then others such as Water at the bottle filling station Water dripping from washer appliances after a cycle The end of an automatic water line Water from hoses Refer to your facility plan list.

21 WATER TESTING How your completed water worksheet should look. It is preferable to Run your Hach AquaChek tests and fill out that part. Then run the swabs and bring swabs and worksheet to RARC for reading. The completed form is scanned and sent back to, so that you have a complete report for your records.

22 AUTOCLAVE TESTING We use the Steris Verify Self Contained Biological Indicators. Your autoclaves are to be tested every month. Wrap the ampule similar to a surgical wrap. Wype-Alls work well. Place an identifying number on each ampule, not on the wrappings. Run 3 ampule packets with a typical load and mark on the worksheet, where each ampule was placed, such as near the vent, center of load, front of load, near the door etc. Ampules need to be incubated within 72 hours (3 days) of being autoclaved. Tests run on Fridays can wait until Monday to go into the incubator, however, do not run the tests on Fridays at the beginning of a 3 day holiday week end.

23 R.A.R.C. COMPARATIVE PATHOLOGY LABORATORY Staff: Pathologists are Dr. Annette Gendron, Dr. Denise Schwahn, Dr. Ruth Sullivan Technicians are Laurel Statz (Research Quality Assurance), Cindy Lundey (Clinical Lab) Phone Numbers: Comparative Pathology Outreach Services Web Address:

24 REFER BACK TO THIS MODULE Ø You may use this module to familiarize and train personnel to use the swabs. Ø You may always call RARC for help with your sanitation monitoring. Ø The modules are available on the RARC, Comparative Pathology Lab web site.

25 THANK YOU ALL FOR YOUR ATTENDANCE