Multi-product Integrated biorefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties ( )

Transcription

1 Production of specialties for food, aquaculture and non-food applications via multi-product biorefinery of microalgae Progress of the EU FP7 project MIRACLES Multi-product Integrated biorefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties ( ) Hans Reith, Leen Bastiaens, Hans Kleivdal, Lolke Sijtsma, Philippe Willems, Carlos Unamunzaga, Elke Breitmayer, Macarena Sanz, René Wijffels AlgaEurope, Madrid, December 2016

2 Objectives MIRACLES Improving cost-effectiveness of production and processing Multiple-product biorefinery Development of new products

3 The MIRACLES CONSORTIUM: 26 partners 6 Universities, 5 Research Organisations, 12 SME s, 3 MNI s incl. 11 end user companies in target sectors In 6 EU countries + Norway + Chile.

4 Production and harvesting Make use of an existing commercial production facility (Fitoplancton Marino) Growth of commercial algae Nannochloropsis Isochrysis Phaeodactylum Scenedesmus Break through technologies Harvesting in membranes and medium recirculation New strains CO 2 from air Novel reactor systems

5 Bioprospecting, metabolic optimization, and cultivation at extreme locations Objectives: Bioprospecting in extreme climatic conditions Optimize of cultivation under different climatic conditions Metabolic models to optimize productivity Extreme climate conditions push towards evolutionary adaptation High biodiversity with special properties/components Rational approach using industrial needs as basis for screening

6 Bioprospecting efforts and progress WP4 Industrial screening criteria Sampling Enrichment Isolation Cultivation Screening Selection BEA CODE STRAIN % BEA 0024 Rhodomonas sp 0,1% TYPE Phaeodactylum tricornutum 0,2% BEA 0650 Tetraselmis striata 0,3% BEA 0664 Pyrocystis lunula 0,4% BEA 0136 Chlorococcum sp. 0,4% BEA 0620 Erythotrichia cf carnea 0,4% BEA 0097 Tetraselmis striata 0,8% BEA 0304 Dunaliella sp 1,3% BEA 0078 Tetraselmis convolutae 1,4% BEA 0163 Dunaliella sp 1,4% BEA 0808 Closteriun sp. 1,7% BEA 0124 Rhodomonas sp 1,9% BEA 0398 Picochlorum oklahomense 2,0% BEA 0615 Graesiella vacuolata 2,0% BEA 0788 Coelastrella saipanensis 2,1% TYPE Scenedesmus obliquus 2,2% BEA 0274 Crucigeniella sp 2,3% BEA 0317 Desmodesmus sp. 2,4% BEA 0544 Coccomyxa sp 2,4% BEA 0406 Cosmarium sp 2,8% BEA 0316 Desmodesmus sp. 3,0% BEA 0364 Protodesmus globulifer 3,4% BEA 0069 Halochlorella rubescens 4,5% TYPE Isochrysis galbana 7,1% BEA 0313 Sarcinochrysis marina 8,4% TYPE Nannochloropsis gaditana 10,5% BEA 1048 Euglena sp. 22,0% BEA 0937 Euglena cantabrica 57,5% CONTROL Yeast 46% ß-Glucan 44,6% WP3 Downstream processing Environmental samples collected Enrichments cultures in lab Clonal cultures plated (FACS) Clonal isolates established for cultivation Screened strains Candidate strains Sub-tropics, Gran Canarias, Spain Altiplanic lagoon Antofagasta, Chile WP4 Application testing WP5 Demonstration Arctic/Nordic, Bergen, Norway Total Candidates for Food applications: 11 Candidates for Feed applications: 7 Candidates for Speciality chemicals: 3 Candidates for Materials applications: 6

7 D ry w e ig h t (g l - 1 ) C Evaluating strain cultivation under the different climatic conditions µ m o l p h o to n s m - 2 s P 1 P Pilots in extreme environments Bergen, Norway Las Palmas, Gran Canary Islands Antofagasta, Chile P 1 P 2 P Time (days)

8 CO 2 capacity (mol/kg) CO 2 concentration from ambient air air? CO 2 Independent from CO 2 point source Screening sorbents Cost target: < 75 /ton CO 2 Activities Sorbent screening Sorbent characterisation CO 2 adsorption capacity H 2 O co-adsorption Kinetics Sorbent stability Regeneration Process & Contactor design Prototype / Proof of Concept at lab scale Universiteit Twente. (NL) Adsorption equilibria ppm CO2 exp.data LINE = FIT

9 2 Contactor Design Gas-sorbent contacting (basic types) Cyclic operation (a) (b) (c) Fixed bed Fluid bed Parallel flow vs Continuous operation Treated gas Flue gas d 1 cm Transport gas Cooling water Adsorption Column (many other configurations possible) Riser Rotary valve Cyclone Rotary valve Cooling water Energy input: LOW Residence time: Gas : SHORT Particle : LONG CO 2 removal efficiency: HIGH Sorbent working capacity : HIGH Regeneration and reuse of absorbant: HIGH Multistage fluid bed Rotary valve Transport gas Fluidization gas CO + Fluidization gas Gas-solid trickle flow reactor (UTwente) Desorption Column

10 Contactor design & testing Contactor design Radial Flow Contactor Convective sorbent/air contact Low DP Using fixed bed (FB) results for design in good agreement Construction + Testing Design of lab scale unit (0.5 kg CO 2 /day)

11 Development Liquid Foam Bed PhotoBioReactor Concept: growth of algae in liquid foams. Foam generation controlled by foaming agents and gas distributors Feature Very short light path Limited amount of water in reactor Low weight, low pressure drop, Large interface surface area Increased gas residence time Consequence High biomass conc. Reduced harvesting costs Reduced construction costs, energy costs Enhanced mass transfer CO 2, O 2

12 Proof of principle Liquid recirculation to the top No foam breaker

13 Development of integrated biorefinery Multiple products Complete cell disruption (bead milling and homogenization) Extraction with supercritical fluids Innovations Milder disruption techniques: PEF, enzymes Membrane separation

14 Product development and market assessment Develop and validate applications for microalgae Characterisation Functionality testing Formulation Performance testing Market analysis Create a market pull Orient tasks of WP1, 2, 3 & 5

15 Products developed/in the pipeline Aquafeed pellets Food & Nutraceuticals Feed & Aquafeed Personal care Anti-microbial & anti-oxidant Thermoplastic biopolymers Thermosetting resins &glues Fertilisers Glue for wood panels Biopolymers Decorative panels

16 Conclusions Multiple products: ambitious 4 products developed, new products coming Breakthrough technologies Promising new strains Proof of principle of CO 2 capture from air Proof of principle foam reactor