A Study of Composting System of Municipal Solid Waste with Bio-surfactant

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1 The Journal of American Science, (), 5, Xi, Study of Composting System of Municipal Solid Waste A Study of Composting System of Municipal Solid Waste with Bio-surfactant Beidou Xi, Yingjun Li, Zimin Wei,, Yue Zhao, Hongliang Liu. Chinese Research Academy of Environmental Science, Beijing, China; Xibeidou@6.net. Beijing Vocational Agriculture, Beijing, China. Northeast Agriculture University, Harbin, Heilongjiang 5, China; Weizm69@6.com Abstract: Three sets of adding Bio- surfactant experiments were conducted to understand composting processes with bio-surfactant. Inoculating Strains Ⅲ(), Pseudomonas aeruginosa (PA) and Bio-surfactant to MSW system by for Run, Run, Run, respectively. The composting experiments showed that: The system of treatment (.4 Strains Ⅲ()), reduce surface tension between liquid and solid to 6 mn/ml at 4 hours. Compared with the control, the quantity of humic from.6 to 8. and accumulation H S of outlet gas was around half of the control. Thus, Inoculation composting technology with bio-surfactant is a promised method to enhance composting efficiency and improve composting quality. [The Journal of American Science, ():66-7]. Keywords: municipal solid waste; composting; bio-surfactant; efficiency composting process. Introduction Composting is a well-know system for rapid organic matter (OM) stabilization and humification (Adani, 995; Desai J D, 997), and Compost is organic fertilizer containing primary nutrients as well as trace minerals, humus and humic acids, in a proportion that almost exactly matches plant requirements, and in a slow release form that does not burn plants. At present, because of the poor compost technology in China, some problems, such as, low efficiency of MSW compost, unstable production qualities, and some strong smells, made a lot of compost factory be a state of stop production and half stop production owing to the unmarketable of compost production, worse environment of compost plant, and expensive fee of MSW treatment (Nakano, 99), therefore, how to increase the efficiency of compost, improve the environment and qualities of compost have become the technological keys. Bio-surfactant comes from the metabolic product of microorganisms; it can improve the microenvironment of compost, promote the dissolution of infectant and accelerate the process of compost reaction. In this paper, the further study on strengthen compost technology by Bio-surfactant has important theory and practice meaning on popularization of MSW compost in China.. Materials and Methods. Materials Because of the complex and unsteadiness of MSW compositions, primary MSW compost would produce many uncertainty factors. In this paper, compost materials are made up of many substances according to a certainty proportion (Table ), properties of compost materials were showed in Table. Table. The composition of compost materials Materials Potato cabbage meat rice paper soil unit note Contents / Table. Properties of compost materials Treatment Carbon Water Ash matter Nitrogen Phosphorus Potassium C/N ph Compost ~5 5~6 5~5 55~65.~.5?? 4~ 6.5~7 Materials ?

2 The Journal of American Science, (), 5, Xi, Study of Composting System of Municipal Solid Waste Culture medium compositions: peptone, NaCl, K HPO 4, agaragar, potato distill juice, glucose, wort, barm juice, distilled waster, et al. Experiment instruments: surface tension instrument, TOC analysis instrument, culture box, compost reactor, O -H S detection instrument, CO detection instrument (ANALYZER LX-7) (Zhang, ).. Experiment methods kg compost materials (water content 55-6) put into compost reactor (Figure ) designed with a total volume of 7 L, and mm in length, mm in width, and mm height. Additional equipments: churn-dasher, ph probe, gas flow meter, temperature maintenance box, an O -H S monitoring instrument (Model MD-5E), and a CO analyzer (Model LX-7). The experimental design included 4 treatments (Table ). The experimental strains included strainsiii () and pseudomonas aeruginosa (PA) which produce bio-surfactant strongly. The different strains were respectively inoculated in 5 ml corresponding culture medium, and cultured by shake for 4 h at 8. And the suspension was inoculated in composting. Inoculating concentrations of the suspension were shown in Table. The bio-surfactant of treatment 4 was purified from the culture solution of strainsiii (). During composting, a g sub-samples was taken once a day. A g was analyzed for moisture content and ash. A g was extraction by Na P 4 O 7 solution, and 5 matters 4 6 analyzed humus. A g was added to distilled waster, and analyzed surface tension and ph. Table. Quantity of Bio- surfactant accession in Trentments compost materials Additive Control () -- Dose, w/w Strainsβ( ).4 Pseudomonas A eruginosa (PA) --.4 Bio-surfactant.8. Analyses methods.. Analyses items and methods () Surface tension measurement To g fresh MSW sample and 9 ml of distilled water was added, And the solution was shaken ( rpm, 7 ) for h, the suspension was then centrifuged (4 r min - ) for min and the supernatant was filtered through a.45-um membrane filter, then measured by surface tension instrument (Xi, ). () Air feed quantity and gas concentration outlet tested by O -H S detection instrument and CO detection instrument, respectively... Calculated methods Oxygen consumption rate can be defined as the consumption oxygen quantity of per unit time in dry compost materials degradation. Oxygen consumption rate and accumulated oxygen consumption profile can R = A a t q( zi z V M e ) dt be calculated by the gas difference of inlet to outlet (Haug, 99). Where, R is the oxygen consumption rate (mol (h kg) - ); q is the airflow (m h - ); z e is O concentration of outlet gas (mol kg - ); z i is O concentration of inlet gas (mol kg - ); V M is the volume of per mol gas (mol mol - ); M d is the weight of dry matter (kg); a is the oxygen quantity of per unit organic matter degradation (.7g kg); A o is the molecular weight of oxygen (. kg mol). in Figure. Schematic diagram of experimental reactor air pump.gas flow meter.composting tank 4.temperature maintainence box 5. Gas analyzer 6.filter. Results. Surface tension profile during composting The surface tension profile was showed at fig., 67

3 The Journal of American Science, (), 5, Xi, Study of Composting System of Municipal Solid Waste during composting, the surface tension of declined to the minimal value (46.5mN m - ) at 7 h, and could maintain under 5 mn m - only for 6 h; but that of adding strain III (treatment ), pseudomonas aeruginosa (treatment ) and bio-surfactant (treatment ) declined to the minimal value (6. mn m -, 6.5 mn m -, 9.8 mn m - ) and maintain under 5 mn m - for 8 h, 96h and 96h, respectively. The results showed, to a certainty range, the surface tension related to the adding strains which could produce the surfactant, or directly adding bio-surfactant. In this study, the decline range of surface tension at treatment and were lower than that of and treatment during composting. And ordered as follow: treatment, treatment > treatment >. the results suggested, the inoculating strains which selected from composting, could regulate metabolism according to surroundings, and produce relevant surfactant to reduce the surface tension between liquid and solid, then make the substance easily to be extracted from inherent of solid waste. Compared to adding strains, the ability of adapting composting system by directly adding bio-surfactant was limited. Therefore, to declined the surface tension between liquid and solid during composting, inoculating microbes which could produce surfactant, is a effective way. Surface rensiion/(mn/m) t/h Fig. Curve changes of surface tension during composting. Oxygen consumption rate and accumulation oxygen consumption The Figure and Figure 4 showed, Oxygen consumption rates and accumulation oxygen consumption of treatment, and all increased at a different degree compared to. For, treatment, treatment, and treatment, the maximal oxygen consumption rates were. mol (h kg) - at 96 h,.6 mol (h kg) - at 48 h,.5 mol (h kg) - at 7 h,.5 mol (h kg) - at 7 h, and the accumulation oxygen consumption were.5 g kg -, 46. g kg -, 44.8 g kg -, 8.7 g kg - with 4 h operation, respectively. The results showed that inoculating microbes on composting could increase the oxygen consumption rate rapidly, and the maximal oxygen consumption rate was.6 times of t /h Figure. Curve changes of oxygen conxumption rate t/h Figure 4. Curve changes of oxygen consumption accumulation rate. CO and H S evolution of outlets gas CO and H S evolution of outlets gas could further study the compost proceed. During composting, the CO and H S concentration was measured (Table 4). CO concentration of treatment and reached up to.9, 9. at 48 h, respectively, and was - times of (4.6). During composting, HS concentration in was highest among all treatments, whose maximal emission was 6. ml m -, while the concentration in treatment, and was.5 ml m -, 5.4 ml m -, 8.8 ml m -, respectively. The results indicted adding bio-surfactant could incline the surface tension between liquid and solid, increase the translation of organic matter, improve the surroundings of composting, and then restrain the emission of malodor gases. The orders were as followed: StrainsⅢ()> PA> Bio-surfactant, the reason for this was the inoculated microbes selected from composting system, could adapt the composting matrix environmental quickly, and produce 68

4 The Journal of American Science, (), 5, Xi, Study of Composting System of Municipal Solid Waste bio-surfactants incessantly during composting process. The activity of directly adding bio-surfactant weakened owing to the limited ability to adapting composting environmental. Times Table 4. Density changes of CO H S by composting equipment export Outlet CO () Inlet H S (ml m - ) (h) Control Control Table 5. Compare of compost quality Treatments Humus(C,) TN() TP(P O 5,) TK(K O,) Date rate of ova() Colibacillus value Control ~ ~ -.4 compost quality The product qualities including humus, TN, TP, TK, death rate of ova and colibacillus are investigated and listed in Table 5. Table 5 showed the humic concentration of inoculating microbes treatment and increased compared with that of control. The total nutrition (N, P and K) were.75,.75, and were.8,.5 times of that in control (.8), respectively. Therefore, inoculating microbes which excrete bio-surfactant on composting not only can enhance composting rate, but also can improve the composting quality greatly.. Conclusions In conclusion, we observed that inoculating Strains Ⅲ(), Pseudomonas aeruginosa which could produce bio-surfactant, can not only accelerate the composting reaction rate but also improve the quality of compost. The paper analyzed the changes of concentration of outlet gases, surface tension, and compost quality in different treatments during static state composting, the result indicated, the effect on composting efficiency of different treatments were as followed, strains III() > pseudomonas aeruginosa > bio-surfactant > control. Acknowledgement: Foundation item: National Basic Research Program of China (5CB74, C48-7, CB4). Correspondence to: Beidou Xi, Zimin Wei Chinese Research Academy of Environmental Sciences Beijing, China Telephone: Cellular phone: 86-87, Xibeidou@6.net, Weizm69@6.com References. Adani F, Gennevini PL, Gasperi F, et al, 995. A new index of organic matter stability [J]. Compost Sci Util 995;:5-7.. Zhang BY, Huang G, Chen B, et al. Study on mechanisms of Biosurfactant-enhanced composting technology for waste management [C]. Annual conference of the Canadian Society for Civil Engineering. Moncton, Noureau-Brunswick, Canada 4-7 June /June 4-7:. 69

5 The Journal of American Science, (), 5, Xi, Study of Composting System of Municipal Solid Waste Desai JD, Banat IM. Microbial Production of Surfactants and Their Commercial Potential [J]. Microbial Molec Biol Rev 997;6:-47. Haug RT. Development of Simulation Models, In: The Practical Handbook of Compost Engineering[M], Lewis Publishers, 99:-6. Nakano MM, Corbell N, Besson J, Zuber P. Isolation and characterization of sfp: a gene that functions in the production of the lipopeptide biosurfactant, surfactin, in Bacillus subtilis [J]. Mol Gen Genet 99;:. 6. Xi BD, Meng W, Huang GH, et al. Composting technology of municipal solid waste with inoculation agent [J]. Environmental Science ;4():

Continuous bioremediation of phenol polluted air in an external loop airlift bioreactor with packed bed Hossein Nikakhtari 1 and Gordon A.

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