In Vivo Hazard Containment: Equipment, Facilities i i & Practices

Transcription

1 Mid Atlantic Biological Safety Association 24 th Annual Biological Safety Symposium In Vivo Hazard Containment: Equipment, Facilities i i & Practices Neil S. Lipman Memorial Sloan Kettering Cancer Center Weill Medical College of Cornell University

2 Hazardous Agents: Animal Models Biological i l agents microorganisms toxins Hazardous Chemicals mutagens/teratogens/abortifactants carcinogens toxins Radionuclides

3 Safety Principles i Laboratory Practice & Technique Safety Equipment (1 barrier) Caging System(s) Biological Safety Cabinet (Class I & II) Chemical Hood Facility (2 barrier)

4 Hazard Considerations Biological i l Shedding Particulate Aerosol Chemical Volatile Particulate Fecal Effluvium Body fluids Metabolites Carcass

5 Barrier (defn): a material object or set of objects that separates, demarcates, or serves as a barricade

6 Barrier Levels Animal Facility caging system ancillary equipment air lock air or wet shower Room/Cubicle air pressure physical boundary filtration Building air pressure physical boundary

7 Keep In, Keep Out (KIKO)

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9 SHOEBOX CAGE

10 KRAFT ISOLATOR CAGE 1958

11 KRAFT BIOLOGICAL TRANSFER HOOD

12 Filter Tops(Historical) p(

13 SEDLACEK MICROISOLATOR CAGE

14 Keller et al LAS 1988

15 Effect of Room RH on Average Weekly Relative Humidity Levels in Static Isolator Cages % Rela ative Hu umidity 80.0% 60.0% 40.0% 20.0% 00% 0.0% 20% RH 50% RH si c room si = static isolator c = control (open shoebox)

16 Microenvironmental Ammonia (weekly average) Levels in Static Isolator Cages Amm monia (ppm) % relative humidity 50% relative humidity mi mb mp c room ml = microisolator mb = microbarrier mp = molded polyester c = control

17 Effect of Caging Systems on Average Weekly Carbon Dioxide Levels Carbo on diox xide ppm ml = microisolator mb = microbarrier mi mb mp c room mp = molded polyester c = control Microenvironmental relatively humidity = 50%

18 Disposable Isolator Cages

19 Secondary Enclosures Ventilated tiltdcbi Cabinets

20 Secondary Enclosures cont. Mass Air Displacement Units

21 High Level Containment

22 Individually Ventilated Cage Systems

23 Ventilated Caging Systems (VCS)

24 VENTILATED CAGING SYSTEMS (VCS)

25 SF6 Gas Release and Capture in Ventilated Cages

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27 VCS HVAC System Integration SUPPLY AIR EXHAUST AIR

28 Ventilated Caging Systems Hazard Containment Intracage Pressure Considerations Decontamination Negative Autoclave Chemical Gas Cage Effluent Filtration HEPA particulate Charcoal-select chemicals Capture-HVAC System Blower Control

29 VCS Biocontainment HEPA exhaust cage/rack Filter DOP testable Blower integration Autoclavable Fully sealed gasketed cage Cages maintained under neg pressure (0.38 H 2 O) Ports gas decon

30 VCS Biocontainment HEPA exhaust cage/rack Filter DOP testable Blower integration Autoclavable Fully sealed gasketed cage Cages maintained under neg pressure (0.38 H 2 O) Ports gas decon

31 VCS Biocontainment HEPA exhaust cage/rack Filter DOP testable Blower integration Autoclavable Fully sealed gasketed cage Cages maintained under neg pressure (0.38 H 2 O) Ports gas decon

32 Biological Safety Cabinets Animal Care and Use Class I cabinet personnel protection exhaust HEPA filtered cage dump station carbon filter +/ hard/thimble connect Class II cabinet product and personnel protection exhaust HEPA filtered Types A2 & B2 expanded sash opening

33 BSC Modifications Animal Use

34 BSC Limits: Cage Changing 1/6 6/6 6/6 0/6 1/6 2/6 Plan View: 5 Class II Type A2 BSC = Settle Plate

35 Biosafety Levels Level 1 Description Agents not known to cause disease in healthy adults. Recm d Caging System Open shoebox cages Static isolator cages Ventilated caging systems Agents associated with 2 Static isolator cages human disease. Select ventilated caging Transmission - autoinoculation, ingestion, systems mucous membrane exposure. e.g. Salmonella +/- Class II BSC spp. & Shigella spp.

36 Biosafety Levels (continued) Level 3 Description Indigenous or exotic agents which may cause disease with serious or lethal consequences Aerosol transmission possible e.g. M. tuberculosis, C. burnetti, LCM Recm d Caging System Static isolator cages + MADU (neg) Static isolator cages + cubicle (neg) Static isolator cages + ventilated cabinet (neg) Select ventilated caging systems?????? +Cl Class IIbi biological i lsafety cabinet 4 Dangerous/exotic agents which pose high risk of life-threatening disease Specialized systems

37 Biocontainment Animal Facilities

38 Typical Biohazard Suite Floorplan

39 BIOCONTAINMENT SUITE Cubicles: Pre manufactured & BIOCONTAINMENT SUITE Constructed

40 Biocontainment Cubicle Airflow HEPA FILTER AT ROOF SUPPLY AIR EXHAUST AIR LOW VOLUME FRONT REAR

41 Biocontainment Cubicle Airflow (cont) Supply and exhaust into room Direct connect supply and exhaust HVAC system Variable air exchange rates (volatile agents ~100 ACH)

42 Cubicles Details

43 Configurable BSL 3/ABSL 3 Laboratory High Containment Low Containment

44 Configurable BSL 3/ABSL 3 Laboratory High Containment Low Containment

45 Configurable BSL 3/ABSL 3 Laboratory High Containment Low Containment

46 Configurable BSL 3/ABSL 3 Laboratory High Containment Low Containment

47 Configurable BSL 3/ABSL 3 Laboratory High Containment Low Containment

48 Configurable BSL 3/ABSL 3 Laboratory High Containment Low Containment

49 In Vivo Hazardous Agent Identification IACUC

50 Hazardous Agent Review Environmental Health & Safety or Radiation Safety Specialist + Attending Vet Select IACUC protocol sections Training, practices, PPE & equipment, waste disposal, etc. specified P&C form generated and signed by review staff/pi Reviewed with applicable staff Posted with relevant protocol sections when study is active

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