The National Algaepilot Mongstad:

Transcription

1 The National Algaepilot Mongstad: production of microalgae for food and feed Dorinde Kleinegris, Hanna Böpple, Pia Steinrücken, Svein Rune Erga, Hans Kleivdal

2 m3/ha/year Why microalgae? High diversity many products Oil Productivity 25,8 High areal productivity 20 CO 2 mitigation No arable land Low water use Fresh water, seawater and wastewater ,4 1,0 1,2 1,3 1,9 6,0 12,3 Steer metabolism towards product of interest

3 Main components Microalgae Lipids Carbohydrates Proteins Other valuable compounds Storage lipids Mainly TAGs Up to 50% of DW Membrane lipids Different lipid classes (phospholipids, galactolipids,...) Up to 40% of total lipids are PUFA Storage products A-(1,4)-glucans B-(1,3)-glucans Fructans Sugars, glycerol Exopolysaccharides: galactose, fucose, rhamnose, arabinose, xylose, glucose High content, up to 50% of DW All 20 amino acids Pigments Antioxidants Vitamins Anti - Fungal - Microbial - Viral Toxins Sterols MAAs

4 Some microalgae products currently available in Norway

5 Still a gap to bridge for commodities from microalgae

6 Commercial production challenges: Product costs Scale Production chain analysis Market development

7 Food Protein & Omega-3 &Protein Omega-3 & Protein Directly utilize the source of nutrients from the bottom of the chain

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9 Roadmap for microalgae based aquafeed (2013) Algae biology Screening & Develop new prod strains Optimize cultivation conditions Upscale production Processing Feed formulation and development Demonstrate functionality University of Bergen Uni Research SINTEF F&A University of Tromsø SINTEF M&C NTNU Nofima Polarfeed Skretting EWOS NTNU UiS CORE NIVA NMBU MicroA Biomar Marine Harvest

10 Roadmap for microalgae based aquafeed (2013) Algae biology Screening & Develop new prod strains Optimize cultivation conditions Upscale production Processing Feed formulation and development Demonstrate functionality University of Bergen Uni Research SINTEF F&A Nofima University of Tromsø Polarfeed SINTEF M&C NTNU NMBU UiS CORE NIVA National Algepilot Mongstad - Connect research with industry - Strenghten competence & capacity - Produce sufficient volumes of algae for application testing Skretting EWOS Biomar NTNU MicroA Marine Harvest

11 National Algaepilot Mongstad National platform for the industrialization of microalgae Owned by UiB Managed by UiB, NORCE and CO2BIO Build in 2016 Operational since 2017 Nextdoor to Equinor refinery and TCM Financed by: University of Bergen Seafood Research Fund Government Hordaland County Municipality councils 6 mnok 3 mnok 6 mnok 2 mnok 1 mmok 18 mnok

12 We develop the full microalgae production chain: from bioprospecting to process optimization and biomass production at pilot scale Photo: Thor Brødreskift to development of new valuable products from microalgae

13 Microalgae + UiB CO2FOOD Algae2Future Waste2Use IFishIENCI

14 Miracles Aquaculture feed Food & Nutraceuticals Non-food specialties

15 Bioprospecting + strain selection Nordic climate Search for: strains with high EPA/DHA content. Assumption: Microalgae from northern high latitudes are expected promising candidates, as low growth temperatures can increase fatty acid unsaturation. Sampling Isolation Cultivation Screening Selection 58 events 110 plates 149 clonal cultures 30 strains: Growth Fatty acids Promising strains: growth rates > 0.7 d -1 EPA/DHA >3% DW 8 selected for further characterization

16 Bioprospecting + strain selection Nordic climate Comparison under Nordic outdoor conditions in flat-panel PBRs Compared vs. commercial Phaeodactylum reference strain m o l p h m - 2 d A I r r a d i a n c e B B i o m a s s p r o d u c t i o n g L - 1 d F it o M 2 8 B C E P A p r o d u c t i o n 1 0 m g L - 1 d s p r i n g s u m m e r a u t u m n

17 Bioprospecting + strain selection Nordic climate A principal component analysis (PCA) of the average seasonal fatty acid compositions (%TFA) revealed strainspecific fatty acid profiles and only little influence of the season on the fatty acid composition. However, adding fatty acid data from laboratory experiments to the PCA, revealed more significant differences between outdoor- and laboratory-grown cultures than between strains.

18 Microalgae show great potential as functional ingredients in aquaculture feeds Experiments: Nannochloropsis and Phaeodactylum (whole cells, disrupted, residual biomass, oil) Diets with 1 8 % inclusion rates Tested on: salmon, gilthead seabream, senegalese sole Demonstrated benefits on: Animal welfare (stress resistance, immunity, improved survival of larvae) Consumer quality traits (more vivid and typical external pigmentation) Reduce reliance on finite marine-harvested resources (e.g. replacement of fish meal (80%) and fish oil (30%) )

19 Scale - Salmon as example Objective: replace 10% of fish meal with algae 1.3 million tonnes of Atlantic salmon 1.8 million tonnes of feed 0.18 million tonnes of algae Without algae With algae

20 Scale - Salmon as example Objective: replace 10% of fish meal with algae 1.3 million tonnes 1.8 million tonnes 0.18 million tonnes Nutrients needed: tons CO ton N ton P TCM captures ton CO 2 per year Yara produces in Norway alone 2,7 million ton NPK yearly BUT There are other sources as well...

21 Næring Industrial symbiosis for development of new value chains CO 2 CO 2 Insektproduksjon Matavfall

22 Evaluation of different CO 2 sources for microalgae production Tested in 25L LGEM tubular PBRs: 3 sources tech CO 2 TCM CO 2 RFCC TCM CO 2 CHP 3 algae Tetraselmis chuii Nannochloropsis gaditana Phaeodactylum tricornutum Gas-fired PP CO 2 CO 2 CO 2 Refinery Statoil CO 2

23 Evaluation of different CO 2 sources for microalgae production Tested in 25L LGEM tubular PBRs: Equal or better growth on captured CO 2 Still looking into biomass quality Gas-fired PP CO 2 CO 2 CO 2 Refinery Statoil CO 2

24 Preliminary experiments integration with waste streams Municipality Biodegradable Waste Insect manure Fish manure

25 Biomass productivities (g/l/d) WP 1 Establish production strains and commercial cultivation media Waste streams 0,4 Phaeodactylum B58 Biomass Productivity (EXP) Extracts of: Aquaculture waste Fish processing plant 0,2 Municipal waste water Beer brewing waste WUR : standard medium 0,0 For 55ml For 110ml Blod 55ml Blod 110ml Kloakk 55ml Kloakk 110ml Mask 55ml Mask 110ml WUR 55ml WUR 110ml Insekt 82ml Insekt 41ml Insect manure

26 Using algae within the organic waste pyramid

27 Algae for food & feed CO 2

28 Algae for plankton and fish larvae production Other fish than salmon: e.g. Ballan wrasse, more difficult production process Greenwater effect Light filter / create shade Anti-bacterial agent In situ biological filter and produce oxygen Beneficial effects on health Feed / feed enrichment direct or via copepods and rotifers

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30 Thank you for your attention Questions?