: Capture enhancement reagent

Transcription

1 Capture enhancement reagent SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name : Capture enhancement reagent Substance name : Capture enhancement reagent Relevant identified uses of the substance or mixture and uses advised against Use of the Substance/Mixture : Assay reagent 1.3 Details of the supplier of the safety data sheet Company : Janssen Pharmaceutica NV Turnhoutseweg Beerse Belgium Telephone : Telefax : address Responsible/issuing person : SDSJanssen@its.jnj.com 1.4 Emergency telephone number SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification (REGULATION (EC) No 1272/2008) Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Classification (67/548/EEC, 1999/45/EC) Harmful R22: Harmful if swallowed. 2.2 Label elements Labelling (REGULATION (EC) No 1272/2008) Not a hazardous substance or mixture according to Regulation (EC) No. 1272/ Other hazards This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 3: Composition/information on ingredients 3.2 Mixtures Chemical nature : Liquid 1 / 10

2 Capture enhancement reagent Hazardous components Chemical Name CAS-No. EC-No. Registration number sodium azide Classification (67/548/EEC) T+; R28 R32 N; R50-R53 Classification (REGULATION (EC) No 1272/2008) Acute Tox.2; H300 Aquatic Acute1; H400 Aquatic Chronic1; H410 Concentration [%] >= < 0.25 For explanation of abbreviations see section 16. SECTION 4: First aid measures 4.1 Description of first aid measures If inhaled : If breathed in, move person into fresh air. Consult a physician. In case of skin contact In case of eye contact If swallowed : Take off contaminated clothing and shoes immediately. Wash off with plenty of water. If symptoms persist, call a physician. : Rinse immediately with plenty of water, also under the eyelids, for at least 5 minutes. Remove contact lenses. If eye irritation persists, consult a specialist. : If swallowed, rinse mouth with water (only if the person is conscious). Call a physician immediately. 4.2 Most important symptoms and effects, both acute and delayed Symptoms : No information available. 4.3 Indication of any immediate medical attention and special treatment needed Treatment : Treat symptomatically. SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing media : Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. 2 / 10

3 Capture enhancement reagent 5.2 Special hazards arising from the substance or mixture Specific hazards during firefighting : No information available. 5.3 Advice for firefighters Special protective equipment for firefighters Further information : In the event of fire, wear self-contained breathing apparatus. : No information available. SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Personal precautions : In the event of an accidental release the emergency response team must respond based on a risk assessment and use personal protective equipment as appropriate. Evacuate personnel to safe areas. 6.2 Environmental precautions Environmental precautions : Should not be released into the environment. 6.3 Methods and materials for containment and cleaning up Methods for cleaning up : Large spills: Dam up. Soak up with inert absorbent material. Keep in properly labelled containers. Small spills: Gently cover the spill with an absorbent towel or pad. Large spills + Small spills: Keep in suitable, closed containers for disposal. Treat recovered material as described in the section "Disposal considerations". 6.4 Reference to other sections For disposal information, see section 13 SECTION 7: Handling and storage 7.1 Precautions for safe handling Advice on safe handling Advice on protection against fire and explosion Hygiene measures : To avoid thermal decomposition, do not overheat. Avoid inhalation, ingestion and contact with skin and eyes. For personal protection see section 8. : : Handle in accordance with good industrial hygiene and safety 3 / 10

4 Capture enhancement reagent practice. 7.2 Conditions for safe storage, including any incompatibilities Requirements for storage areas and containers Storage temperature : 2-8 C : To maintain product quality, do not store in heat or direct sunlight. Store in original container. Keep containers tightly closed in a dry, cool and well-ventilated place. Keep away from heat and sources of ignition. Keep locked up. Keep refrigerated. 7.3 Specific end use(s) Specific use(s) : Consult the technical guidelines for the use of this substance/mixture. SECTION 8: Exposure controls/personal protection 8.1 Control parameters Components CAS-No. sodium azide Further information sodium azide Further information Value type (Form of exposure) Control parameters Update Basis TWA 0.1 mg/m /39/EC Identifies the possibility of significant uptake through the skinindicative STEL 0.3 mg/m /39/EC Identifies the possibility of significant uptake through the skinindicative 8.2 Exposure controls Engineering measures All personal protective equipment should be based on a risk assessment. Consult a Environment Health Safety expert if necessary. Personal protective equipment Eye protection : No special precautions required. Hand protection Remarks : Disposable gloves 4 / 10

5 Capture enhancement reagent Skin and body protection : No special precautions required. Respiratory protection : Engineering controls should always be the primary method of controlling exposures. If respiratory protective equipment is needed for certain activities, the type as well as the corresponding protection factor will depend upon the risk assessment and air concentrations, hazards, physical and warning properties of substances present. No personal respiratory protective equipment normally required. Protective measures : The type of protective equipment must be selected based on the Environmental Health and Safety risk assessment. Consult a Environmental Health and Safety expert if necessary. Environmental exposure controls General advice : Should not be released into the environment. SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties Appearance : liquid Colour : clear Odour : 9.2 Other information SECTION 10: Stability and reactivity 10.1 Reactivity None reasonably foreseeable Chemical stability Stable under recommended storage conditions Possibility of hazardous reactions Hazardous reactions : No dangerous reaction known under conditions of normal use Conditions to avoid 5 / 10

6 Capture enhancement reagent Conditions to avoid 10.5 Incompatible materials Materials to avoid : To avoid thermal decomposition, do not overheat. : Oxidizing agents 10.6 Hazardous decomposition products Hazardous decomposition products : None known. SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Product Acute oral toxicity : Acute toxicity estimate : > mg/kg Method: Calculation method Components: sodium azide : Acute oral toxicity : LD50 Rat: 27 mg/kg Skin corrosion/irritation Serious eye damage/eye irritation Respiratory or skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Toxicity to reproduction/fertility Reprod.Tox./Development/Teratogenicity STOT - single exposure 6 / 10

7 Capture enhancement reagent STOT - repeated exposure Aspiration toxicity SECTION 12: Ecological information 12.1 Toxicity Components: sodium azide : Toxicity to fish Toxicity to daphnia and other aquatic invertebrates Toxicity to algae Toxicity to bacteria : LC50 (Lepomis macrochirus (Bluegill sunfish)): 0.7 mg/l Exposure time: 96 h : EC50 (Daphnia pulex (Water flea)): 4.2 mg/l Exposure time: 96 h : IC50 : 272 mg/l : EC50 (Photobacterium phosphoreum): 38.5 mg/l 12.2 Persistence and degradability 12.3 Bioaccumulative potential 12.4 Mobility in soil 12.5 Results of PBT and vpvb assessment Product: Assessment 12.6 Other adverse effects : This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 13: Disposal considerations 13.1 Waste treatment methods 7 / 10

8 Capture enhancement reagent Product Contaminated packaging : In accordance with National, Federal, State and Local regulations. : Empty containers should be taken to an approved waste handling site for recycling or disposal. SECTION 14: Transport information 14.1 UN number ADN ADR RID IMDG IATA 14.2 Proper shipping name ADN ADR RID IMDG IATA 14.3 Transport hazard class ADN ADR RID IMDG IATA 14.4 Packing group ADN ADR RID 8 / 10

9 Capture enhancement reagent IMDG IATA 14.5 Environmental hazards ADN ADR RID IMDG IATA 14.6 Special precautions for user For personal protection see section Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Major Accident Hazard Legislation 96/82/EC : Update: Not applicable Notification status REACH CH INV TSCA DSL AICS NZIoC ENCS ISHL KECI PICCS IECSC Other regulations : Not in compliance with the inventory : The formulation contains substances listed on the Swiss Inventory : On TSCA Inventory : All components of this product are on the Canadian DSL. : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : Not in compliance with the inventory : Not in compliance with the inventory : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : Restricted to professional users Chemical Safety Assessment A Chemical Safety Assessment is not applicable (mixture) 9 / 10

10 Capture enhancement reagent SECTION 16: Other information Full text of R-Phrases R28 Very toxic if swallowed. R32 Contact with acids liberates very toxic gas. R50 Very toxic to aquatic organisms. R53 May cause long-term adverse effects in the aquatic environment. Full text of H-Statements H300 Fatal if swallowed. H400 Very toxic to aquatic life. H410 Very toxic to aquatic life with long lasting effects. Full text of other abbreviations Date and Number Formats This document uses the following notation for printing dates and numbers: Date: Dec 31th, 2012 as Numbers: as The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. 10 / 10

11 Anti-CK-FITC/ Anti CD45-APC SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name Substance name : Anti-CK-FITC/ Anti CD45-APC : Anti-CK-FITC/ Anti CD45-APC 1.2 Relevant identified uses of the substance or mixture and uses advised against Use of the Substance/Mixture : Assay reagent 1.3 Details of the supplier of the safety data sheet Company : Janssen Pharmaceutica NV Turnhoutseweg Beerse Belgium Telephone : Telefax : address Responsible/issuing person : SDSJanssen@its.jnj.com 1.4 Emergency telephone number SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification (REGULATION (EC) No 1272/2008) Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Classification (67/548/EEC, 1999/45/EC) Harmful R22: Harmful if swallowed. 2.2 Label elements Labelling (REGULATION (EC) No 1272/2008) Not a hazardous substance or mixture according to Regulation (EC) No. 1272/ Other hazards This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 3: Composition/information on ingredients 3.2 Mixtures Chemical nature : Liquid 1 / 10

12 Anti-CK-FITC/ Anti CD45-APC Hazardous components Chemical Name CAS-No. EC-No. Registration number sodium azide Classification (67/548/EEC) T+; R28 R32 N; R50-R53 Classification (REGULATION (EC) No 1272/2008) Acute Tox.2; H300 Aquatic Acute1; H400 Aquatic Chronic1; H410 Concentration [%] >= < 0.25 For explanation of abbreviations see section 16. SECTION 4: First aid measures 4.1 Description of first aid measures If inhaled : If breathed in, move person into fresh air. Consult a physician. In case of skin contact In case of eye contact If swallowed : Take off contaminated clothing and shoes immediately. Wash off with plenty of water. If symptoms persist, call a physician. : Rinse immediately with plenty of water, also under the eyelids, for at least 5 minutes. Remove contact lenses. If eye irritation persists, consult a specialist. : If swallowed, rinse mouth with water (only if the person is conscious). Call a physician immediately. 4.2 Most important symptoms and effects, both acute and delayed Symptoms : No information available. 4.3 Indication of any immediate medical attention and special treatment needed Treatment : Treat symptomatically. SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing media : Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. 2 / 10

13 Anti-CK-FITC/ Anti CD45-APC 5.2 Special hazards arising from the substance or mixture Specific hazards during firefighting : No information available. 5.3 Advice for firefighters Special protective equipment for firefighters Further information : In the event of fire, wear self-contained breathing apparatus. : No information available. SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Personal precautions : In the event of an accidental release the emergency response team must respond based on a risk assessment and use personal protective equipment as appropriate. Evacuate personnel to safe areas. 6.2 Environmental precautions Environmental precautions : Should not be released into the environment. 6.3 Methods and materials for containment and cleaning up Methods for cleaning up : Large spills: Dam up. Soak up with inert absorbent material. Keep in properly labelled containers. Small spills: Gently cover the spill with an absorbent towel or pad. Large spills + Small spills: Keep in suitable, closed containers for disposal. Treat recovered material as described in the section "Disposal considerations". 6.4 Reference to other sections For disposal information, see section 13 SECTION 7: Handling and storage 7.1 Precautions for safe handling Advice on safe handling Advice on protection against fire and explosion Hygiene measures : To avoid thermal decomposition, do not overheat. Avoid inhalation, ingestion and contact with skin and eyes. For personal protection see section 8. : : Handle in accordance with good industrial hygiene and safety 3 / 10

14 Anti-CK-FITC/ Anti CD45-APC practice. 7.2 Conditions for safe storage, including any incompatibilities Requirements for storage areas and containers Storage temperature : 2-8 C : To maintain product quality, do not store in heat or direct sunlight. Store in original container. Keep containers tightly closed in a dry, cool and well-ventilated place. Keep away from heat and sources of ignition. Keep locked up. Keep refrigerated. 7.3 Specific end use(s) Specific use(s) : Consult the technical guidelines for the use of this substance/mixture. SECTION 8: Exposure controls/personal protection 8.1 Control parameters Components CAS-No. sodium azide Further information sodium azide Further information Value type (Form of exposure) Control parameters Update Basis TWA 0.1 mg/m /39/EC Identifies the possibility of significant uptake through the skinindicative STEL 0.3 mg/m /39/EC Identifies the possibility of significant uptake through the skinindicative 8.2 Exposure controls Engineering measures All personal protective equipment should be based on a risk assessment. Consult a Environment Health Safety expert if necessary. Personal protective equipment Eye protection : No special precautions required. Hand protection Remarks : Disposable gloves 4 / 10

15 Anti-CK-FITC/ Anti CD45-APC Skin and body protection : No special precautions required. Respiratory protection : Engineering controls should always be the primary method of controlling exposures. If respiratory protective equipment is needed for certain activities, the type as well as the corresponding protection factor will depend upon the risk assessment and air concentrations, hazards, physical and warning properties of substances present. No personal respiratory protective equipment normally required. Protective measures : The type of protective equipment must be selected based on the Environmental Health and Safety risk assessment. Consult a Environmental Health and Safety expert if necessary. Environmental exposure controls General advice : Should not be released into the environment. SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties Appearance : liquid Colour : clear 9.2 Other information SECTION 10: Stability and reactivity 10.1 Reactivity None reasonably foreseeable Chemical stability Stable under recommended storage conditions Possibility of hazardous reactions Hazardous reactions : No dangerous reaction known under conditions of normal use Conditions to avoid Conditions to avoid : To avoid thermal decomposition, do not overheat. 5 / 10

16 Anti-CK-FITC/ Anti CD45-APC 10.5 Incompatible materials Materials to avoid : None known Hazardous decomposition products Hazardous decomposition products : None known. SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Product Acute oral toxicity : Acute toxicity estimate : > mg/kg Method: Calculation method Components: sodium azide : Acute oral toxicity : LD50 Rat: 27 mg/kg Skin corrosion/irritation Serious eye damage/eye irritation Respiratory or skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Toxicity to reproduction/fertility Reprod.Tox./Development/Teratogenicity STOT - single exposure STOT - repeated exposure 6 / 10

17 Anti-CK-FITC/ Anti CD45-APC Aspiration toxicity SECTION 12: Ecological information 12.1 Toxicity Components: sodium azide : Toxicity to fish Toxicity to daphnia and other aquatic invertebrates Toxicity to algae Toxicity to bacteria : LC50 (Lepomis macrochirus (Bluegill sunfish)): 0.7 mg/l Exposure time: 96 h : EC50 (Daphnia pulex (Water flea)): 4.2 mg/l Exposure time: 96 h : IC50 : 272 mg/l : EC50 (Photobacterium phosphoreum): 38.5 mg/l 12.2 Persistence and degradability 12.3 Bioaccumulative potential 12.4 Mobility in soil 12.5 Results of PBT and vpvb assessment Product: Assessment 12.6 Other adverse effects : This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 13: Disposal considerations 13.1 Waste treatment methods Product : In accordance with National, Federal, State and Local regulations. 7 / 10

18 Anti-CK-FITC/ Anti CD45-APC Contaminated packaging : Empty containers should be taken to an approved waste handling site for recycling or disposal. SECTION 14: Transport information 14.1 UN number ADN ADR RID IMDG IATA 14.2 Proper shipping name ADN ADR RID IMDG IATA 14.3 Transport hazard class ADN ADR RID IMDG IATA 14.4 Packing group ADN ADR RID IMDG 8 / 10

19 Anti-CK-FITC/ Anti CD45-APC IATA 14.5 Environmental hazards ADN ADR RID IMDG IATA 14.6 Special precautions for user For personal protection see section Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Major Accident Hazard Legislation 96/82/EC : Update: Not applicable Notification status REACH CH INV TSCA DSL AICS NZIoC ENCS ISHL KECI PICCS IECSC Other regulations : Not in compliance with the inventory : The formulation contains substances listed on the Swiss Inventory : On TSCA Inventory : All components of this product are on the Canadian DSL. : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : Not in compliance with the inventory : Not in compliance with the inventory : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : Restricted to professional users Chemical Safety Assessment A Chemical Safety Assessment is not applicable (mixture) 9 / 10

20 Anti-CK-FITC/ Anti CD45-APC SECTION 16: Other information Full text of R-Phrases R28 Very toxic if swallowed. R32 Contact with acids liberates very toxic gas. R50 Very toxic to aquatic organisms. R53 May cause long-term adverse effects in the aquatic environment. Full text of H-Statements H300 Fatal if swallowed. H400 Very toxic to aquatic life. H410 Very toxic to aquatic life with long lasting effects. Full text of other abbreviations Date and Number Formats This document uses the following notation for printing dates and numbers: Date: Dec 31th, 2012 as Numbers: as The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. 10 / 10

21 Nucleic acid dye SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name : Nucleic acid dye Substance name : Nucleic acid dye Relevant identified uses of the substance or mixture and uses advised against Use of the Substance/Mixture : Assay reagent 1.3 Details of the supplier of the safety data sheet Company : Janssen Pharmaceutica NV Turnhoutseweg Beerse Belgium Telephone : Telefax : address Responsible/issuing person : SDSJanssen@its.jnj.com 1.4 Emergency telephone number SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification (REGULATION (EC) No 1272/2008) Skin sensitisation, Category 1 H317: May cause an allergic skin reaction. Classification (67/548/EEC, 1999/45/EC) Sensitising R43: May cause sensitisation by skin contact. 2.2 Label elements Labelling (REGULATION (EC) No 1272/2008) Hazard pictograms : Signal word : Warning Hazard statements : H317 May cause an allergic skin reaction. Precautionary statements : Prevention: P261 P272 1 / 11 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray. Contaminated work clothing should not be allowed out of the workplace.

22 Nucleic acid dye P280 Response: P333 + P313 P362 + P364 Disposal: P501 Wear protective gloves. If skin irritation or rash occurs: Get medical advice/ attention. Take off contaminated clothing and wash it before reuse. Dispose of contents/ container to an approved waste disposal plant. Hazardous components which must be listed on the label: mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) 2.3 Other hazards This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 3: Composition/information on ingredients 3.2 Mixtures Chemical nature : Liquid Hazardous components Chemical Name mixture of: 5-chloro-2- methyl-4-isothiazolin-3- one [EC no ] and 2-methyl-2Hisothiazol-3-one [EC no ] (3:1) CAS-No. EC-No. Registration number Classification (67/548/EEC) T; R23/24/25 C; R34 R43 N; R50-R53 Classification (REGULATION (EC) No 1272/2008) Acute Tox.3; H331 Acute Tox.3; H311 Acute Tox.3; H301 Skin Corr.1B; H314 Skin Sens.1; H317 Aquatic Acute1; H400 Aquatic Chronic1; H410 Concentration [%] >= < 0.06 For explanation of abbreviations see section 16. SECTION 4: First aid measures 4.1 Description of first aid measures If inhaled : If breathed in, move person into fresh air. 2 / 11

23 Nucleic acid dye Consult a physician. In case of skin contact In case of eye contact If swallowed : Take off contaminated clothing and shoes immediately. Wash off immediately with plenty of water. If symptoms persist, call a physician. Wash contaminated clothing before re-use. : Rinse immediately with plenty of water, also under the eyelids, for at least 5 minutes. Remove contact lenses. If eye irritation persists, consult a specialist. : If swallowed, rinse mouth with water (only if the person is conscious). Call a physician immediately. 4.2 Most important symptoms and effects, both acute and delayed Symptoms : No information available. 4.3 Indication of any immediate medical attention and special treatment needed Treatment : Treat symptomatically. SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing media : Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. 5.2 Special hazards arising from the substance or mixture Specific hazards during firefighting : No information available. 5.3 Advice for firefighters Special protective equipment for firefighters Further information : In the event of fire, wear self-contained breathing apparatus. : No information available. SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Personal precautions : In the event of an accidental release the emergency response team must respond based on a risk assessment and use personal protective equipment as appropriate. Evacuate personnel to safe areas. 3 / 11

24 Nucleic acid dye 6.2 Environmental precautions Environmental precautions : Should not be released into the environment. 6.3 Methods and materials for containment and cleaning up Methods for cleaning up 6.4 Reference to other sections For disposal information, see section 13 : Large spills: Dam up. Soak up with inert absorbent material. Keep in properly labelled containers. Small spills: Gently cover the spill with an absorbent towel or pad. Large spills + Small spills: Keep in suitable, closed containers for disposal. Treat recovered material as described in the section "Disposal considerations". SECTION 7: Handling and storage 7.1 Precautions for safe handling Advice on safe handling Advice on protection against fire and explosion Hygiene measures : To avoid thermal decomposition, do not overheat. Avoid inhalation, ingestion and contact with skin and eyes. For personal protection see section 8. : : Handle in accordance with good industrial hygiene and safety practice. 7.2 Conditions for safe storage, including any incompatibilities Requirements for storage areas and containers Storage temperature : 2-8 C : To maintain product quality, do not store in heat or direct sunlight. Store in original container. Keep containers tightly closed in a dry, cool and well-ventilated place. Keep away from heat and sources of ignition. Keep locked up. Keep refrigerated. 7.3 Specific end use(s) Specific use(s) : Consult the technical guidelines for the use of this substance/mixture. 4 / 11

25 Nucleic acid dye SECTION 8: Exposure controls/personal protection 8.1 Control parameters Contains no substances with occupational exposure limit values. 8.2 Exposure controls Engineering measures All personal protective equipment should be based on a risk assessment. Consult a Environment Health Safety expert if necessary. Personal protective equipment Eye protection : No special precautions required. Hand protection Remarks : Disposable gloves Skin and body protection : No special precautions required. Respiratory protection : Engineering controls should always be the primary method of controlling exposures. If respiratory protective equipment is needed for certain activities, the type as well as the corresponding protection factor will depend upon the risk assessment and air concentrations, hazards, physical and warning properties of substances present. No personal respiratory protective equipment normally required. Protective measures : The type of protective equipment must be selected based on the Environmental Health and Safety risk assessment. Consult a Environmental Health and Safety expert if necessary. Environmental exposure controls General advice : Should not be released into the environment. SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties 5 / 11

26 Nucleic acid dye Appearance : liquid Colour : clear, light yellow Odour : odourless ph : 7.5 Solubility(ies) Water solubility : soluble 9.2 Other information SECTION 10: Stability and reactivity 10.1 Reactivity None reasonably foreseeable Chemical stability Stable under recommended storage conditions Possibility of hazardous reactions Hazardous reactions : No dangerous reaction known under conditions of normal use Conditions to avoid Conditions to avoid 10.5 Incompatible materials Materials to avoid : To avoid thermal decomposition, do not overheat. : None known Hazardous decomposition products Hazardous decomposition products : None known. SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Components: mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2Hisothiazol-3-one [EC no ] (3:1) : Acute dermal toxicity : LD50 Rabbit: mg/kg Skin corrosion/irritation 6 / 11

27 Nucleic acid dye Components: mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2Hisothiazol-3-one [EC no ] (3:1) : Species: Rabbit Result: Corrosive after 3 minutes to 1 hour of exposure Serious eye damage/eye irritation Respiratory or skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Toxicity to reproduction/fertility Reprod.Tox./Development/Teratogenicity STOT - single exposure STOT - repeated exposure Aspiration toxicity SECTION 12: Ecological information 12.1 Toxicity 12.2 Persistence and degradability 12.3 Bioaccumulative potential 12.4 Mobility in soil 7 / 11

28 Nucleic acid dye 12.5 Results of PBT and vpvb assessment Product: Assessment : This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher Other adverse effects SECTION 13: Disposal considerations 13.1 Waste treatment methods Product Contaminated packaging : In accordance with National, Federal, State and Local regulations. : Empty containers should be taken to an approved waste handling site for recycling or disposal. SECTION 14: Transport information 14.1 UN number ADN ADR RID IMDG IATA 14.2 Proper shipping name ADN ADR RID IMDG IATA 14.3 Transport hazard class ADN 8 / 11

29 Nucleic acid dye ADR RID IMDG IATA 14.4 Packing group ADN ADR RID IMDG IATA 14.5 Environmental hazards ADN ADR RID IMDG IATA 14.6 Special precautions for user For personal protection see section Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Major Accident Hazard Legislation 96/82/EC : Update: Not applicable Notification status REACH CH INV : Not in compliance with the inventory : The formulation contains substances listed on the Swiss Inventory 9 / 11

30 Nucleic acid dye TSCA DSL AICS NZIoC ENCS ISHL KECI PICCS IECSC Other regulations : Not On TSCA Inventory : This product contains the following components that are not on the Canadian DSL nor NDSL. : Not in compliance with the inventory : On the inventory, or in compliance with the inventory : Not in compliance with the inventory : Not in compliance with the inventory : Not in compliance with the inventory : Not in compliance with the inventory : On the inventory, or in compliance with the inventory : Restricted to professional users Chemical Safety Assessment A Chemical Safety Assessment is not applicable (mixture) SECTION 16: Other information Full text of R-Phrases R23/24/25 Toxic by inhalation, in contact with skin and if swallowed. R34 Causes burns. R43 May cause sensitisation by skin contact. R50 Very toxic to aquatic organisms. R53 May cause long-term adverse effects in the aquatic environment. Full text of H-Statements H301 Toxic if swallowed. H311 Toxic in contact with skin. H314 Causes severe skin burns and eye damage. H317 May cause an allergic skin reaction. H331 Toxic if inhaled. H400 Very toxic to aquatic life. H410 Very toxic to aquatic life with long lasting effects. Full text of other abbreviations Date and Number Formats This document uses the following notation for printing dates and numbers: Date: Dec 31th, 2012 as Numbers: as The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific 10 / 11

31 Nucleic acid dye material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. 11 / 11

32 Permeabilization reagent SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name : Permeabilization reagent Substance name : Permeabilization reagent Relevant identified uses of the substance or mixture and uses advised against Use of the Substance/Mixture : Assay reagent 1.3 Details of the supplier of the safety data sheet Company : Janssen Pharmaceutica NV Turnhoutseweg Beerse Belgium Telephone : Telefax : address Responsible/issuing person : SDSJanssen@its.jnj.com 1.4 Emergency telephone number SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification (REGULATION (EC) No 1272/2008) Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Classification (67/548/EEC, 1999/45/EC) Harmful R22: Harmful if swallowed. 2.2 Label elements Labelling (REGULATION (EC) No 1272/2008) Not a hazardous substance or mixture according to Regulation (EC) No. 1272/ Other hazards This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 3: Composition/information on ingredients 3.2 Mixtures Chemical nature : Liquid 1 / 10

33 Permeabilization reagent Hazardous components Chemical Name CAS-No. EC-No. Registration number sodium azide Classification (67/548/EEC) T+; R28 R32 N; R50-R53 Classification (REGULATION (EC) No 1272/2008) Acute Tox.2; H300 Aquatic Acute1; H400 Aquatic Chronic1; H410 Concentration [%] >= < 0.25 For explanation of abbreviations see section 16. SECTION 4: First aid measures 4.1 Description of first aid measures If inhaled : If breathed in, move person into fresh air. Consult a physician. In case of skin contact In case of eye contact If swallowed : Take off contaminated clothing and shoes immediately. Wash off with plenty of water. If symptoms persist, call a physician. : Remove contact lenses. If eye irritation persists, consult a specialist. Rinse immediately with plenty of water, also under the eyelids, for at least 5 minutes. : If swallowed, rinse mouth with water (only if the person is conscious). Call a physician immediately. 4.2 Most important symptoms and effects, both acute and delayed Symptoms : No information available. 4.3 Indication of any immediate medical attention and special treatment needed Treatment : Treat symptomatically. SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing media : Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. 2 / 10

34 Permeabilization reagent 5.2 Special hazards arising from the substance or mixture Specific hazards during firefighting : No information available. 5.3 Advice for firefighters Special protective equipment for firefighters Further information : In the event of fire, wear self-contained breathing apparatus. : No information available. SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Personal precautions : In the event of an accidental release the emergency response team must respond based on a risk assessment and use personal protective equipment as appropriate. Evacuate personnel to safe areas. 6.2 Environmental precautions Environmental precautions : Should not be released into the environment. 6.3 Methods and materials for containment and cleaning up Methods for cleaning up : Large spills: Dam up. Soak up with inert absorbent material. Keep in properly labelled containers. Small spills: Gently cover the spill with an absorbent towel or pad. Large spills + Small spills: Keep in suitable, closed containers for disposal. Treat recovered material as described in the section "Disposal considerations". 6.4 Reference to other sections For disposal information, see section 13 SECTION 7: Handling and storage 7.1 Precautions for safe handling Advice on safe handling Advice on protection against fire and explosion Hygiene measures : To avoid thermal decomposition, do not overheat. Avoid inhalation, ingestion and contact with skin and eyes. For personal protection see section 8. : : Handle in accordance with good industrial hygiene and safety 3 / 10

35 Permeabilization reagent practice. 7.2 Conditions for safe storage, including any incompatibilities Requirements for storage areas and containers Storage temperature : 2-8 C : To maintain product quality, do not store in heat or direct sunlight. Store in original container. Keep containers tightly closed in a dry, cool and well-ventilated place. Keep away from heat and sources of ignition. Keep locked up. Keep refrigerated. 7.3 Specific end use(s) Specific use(s) : Consult the technical guidelines for the use of this substance/mixture. SECTION 8: Exposure controls/personal protection 8.1 Control parameters Components CAS-No. sodium azide Further information sodium azide Further information Value type (Form of exposure) Control parameters Update Basis TWA 0.1 mg/m /39/EC Identifies the possibility of significant uptake through the skinindicative STEL 0.3 mg/m /39/EC Identifies the possibility of significant uptake through the skinindicative 8.2 Exposure controls Engineering measures All personal protective equipment should be based on a risk assessment. Consult a Environment Health Safety expert if necessary. Personal protective equipment Eye protection : No special precautions required. Hand protection Remarks : Disposable gloves 4 / 10

36 Permeabilization reagent Skin and body protection : No special precautions required. Respiratory protection : Engineering controls should always be the primary method of controlling exposures. If respiratory protective equipment is needed for certain activities, the type as well as the corresponding protection factor will depend upon the risk assessment and air concentrations, hazards, physical and warning properties of substances present. No personal respiratory protective equipment normally required. Protective measures : The type of protective equipment must be selected based on the Environmental Health and Safety risk assessment. Consult a Environmental Health and Safety expert if necessary. Environmental exposure controls General advice : Should not be released into the environment. SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties Appearance : liquid Colour : clear Odour : odourless ph : 7.5 Solubility(ies) Water solubility : soluble 9.2 Other information SECTION 10: Stability and reactivity 10.1 Reactivity None reasonably foreseeable Chemical stability Stable under recommended storage conditions Possibility of hazardous reactions 5 / 10

37 Permeabilization reagent Hazardous reactions 10.4 Conditions to avoid Conditions to avoid 10.5 Incompatible materials Materials to avoid : No dangerous reaction known under conditions of normal use. : To avoid thermal decomposition, do not overheat. : Strong acids and strong bases Reducing agents Strong oxidizing agents 10.6 Hazardous decomposition products Hazardous decomposition products : None known. SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Product Acute oral toxicity : Acute toxicity estimate : > mg/kg Method: Calculation method Components: sodium azide : Acute oral toxicity : LD50 Rat: 27 mg/kg Skin corrosion/irritation Serious eye damage/eye irritation Respiratory or skin sensitisation Germ cell mutagenicity Carcinogenicity Reproductive toxicity Toxicity to reproduction/fertility Reprod.Tox./Development/Teratogenicity 6 / 10

38 Permeabilization reagent STOT - single exposure STOT - repeated exposure Aspiration toxicity SECTION 12: Ecological information 12.1 Toxicity Components: sodium azide : Toxicity to fish Toxicity to daphnia and other aquatic invertebrates Toxicity to algae Toxicity to bacteria : LC50 (Lepomis macrochirus (Bluegill sunfish)): 0.7 mg/l Exposure time: 96 h : EC50 (Daphnia pulex (Water flea)): 4.2 mg/l Exposure time: 96 h : IC50 : 272 mg/l : EC50 (Photobacterium phosphoreum): 38.5 mg/l 12.2 Persistence and degradability 12.3 Bioaccumulative potential 12.4 Mobility in soil 12.5 Results of PBT and vpvb assessment Product: Assessment 12.6 Other adverse effects : This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. 7 / 10

39 Permeabilization reagent SECTION 13: Disposal considerations 13.1 Waste treatment methods Product Contaminated packaging : In accordance with National, Federal, State and Local regulations. : Empty containers should be taken to an approved waste handling site for recycling or disposal. SECTION 14: Transport information 14.1 UN number ADN ADR RID IMDG IATA 14.2 Proper shipping name ADN ADR RID IMDG IATA 14.3 Transport hazard class ADN ADR RID IMDG IATA 14.4 Packing group ADN 8 / 10

40 Permeabilization reagent ADR RID IMDG IATA 14.5 Environmental hazards ADN ADR RID IMDG IATA 14.6 Special precautions for user For personal protection see section Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Major Accident Hazard Legislation 96/82/EC : Update: Not applicable Notification status REACH CH INV TSCA DSL AICS NZIoC ENCS ISHL KECI PICCS IECSC Other regulations : Not in compliance with the inventory : The formulation contains substances listed on the Swiss Inventory : Not On TSCA Inventory : All components of this product are on the Canadian DSL. : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : Not in compliance with the inventory : Not in compliance with the inventory : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : On the inventory, or in compliance with the inventory : Restricted to professional users. 9 / 10

41 Permeabilization reagent 15.2 Chemical Safety Assessment A Chemical Safety Assessment is not applicable (mixture) SECTION 16: Other information Full text of R-Phrases R28 Very toxic if swallowed. R32 Contact with acids liberates very toxic gas. R50 Very toxic to aquatic organisms. R53 May cause long-term adverse effects in the aquatic environment. Full text of H-Statements H300 Fatal if swallowed. H400 Very toxic to aquatic life. H410 Very toxic to aquatic life with long lasting effects. Full text of other abbreviations Date and Number Formats This document uses the following notation for printing dates and numbers: Date: Dec 31th, 2012 as Numbers: as The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. 10 / 10

42 Anti-EpCAM ferrofluid SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name : Anti-EpCAM ferrofluid Substance name : Anti-EpCAM ferrofluid Relevant identified uses of the substance or mixture and uses advised against Use of the Substance/Mixture : Assay reagent 1.3 Details of the supplier of the safety data sheet Company : Janssen Pharmaceutica NV Turnhoutseweg Beerse Belgium Telephone : Telefax : address Responsible/issuing person : SDSJanssen@its.jnj.com 1.4 Emergency telephone number SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification (REGULATION (EC) No 1272/2008) Skin sensitisation, Category 1 H317: May cause an allergic skin reaction. Classification (67/548/EEC, 1999/45/EC) Sensitising R43: May cause sensitisation by skin contact. 2.2 Label elements Labelling (REGULATION (EC) No 1272/2008) Hazard pictograms : Signal word : Warning Hazard statements : H317 May cause an allergic skin reaction. Precautionary statements : Prevention: P261 P272 1 / 11 Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray. Contaminated work clothing should not be allowed out of the workplace.

43 Anti-EpCAM ferrofluid P280 Response: P333 + P313 P362 + P364 Disposal: P501 Wear protective gloves. If skin irritation or rash occurs: Get medical advice/ attention. Take off contaminated clothing and wash it before reuse. Dispose of contents/ container to an approved waste disposal plant. Hazardous components which must be listed on the label: mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC no ] and 2-methyl-2H-isothiazol-3-one [EC no ] (3:1) 2.3 Other hazards This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vpvb) at levels of 0.1% or higher. SECTION 3: Composition/information on ingredients 3.2 Mixtures Chemical nature : Liquid Hazardous components Chemical Name mixture of: 5-chloro-2- methyl-4-isothiazolin-3- one [EC no ] and 2-methyl-2Hisothiazol-3-one [EC no ] (3:1) CAS-No. EC-No. Registration number Classification (67/548/EEC) T; R23/24/25 C; R34 R43 N; R50-R53 Classification (REGULATION (EC) No 1272/2008) Acute Tox.3; H331 Acute Tox.3; H311 Acute Tox.3; H301 Skin Corr.1B; H314 Skin Sens.1; H317 Aquatic Acute1; H400 Aquatic Chronic1; H410 Concentration [%] >= < 0.06 For explanation of abbreviations see section 16. SECTION 4: First aid measures 4.1 Description of first aid measures If inhaled : If breathed in, move person into fresh air. 2 / 11

44 Anti-EpCAM ferrofluid Consult a physician. In case of skin contact In case of eye contact If swallowed : Take off contaminated clothing and shoes immediately. Wash off immediately with plenty of water. If symptoms persist, call a physician. Wash contaminated clothing before re-use. : Rinse immediately with plenty of water, also under the eyelids, for at least 5 minutes. Remove contact lenses. If eye irritation persists, consult a specialist. : If swallowed, rinse mouth with water (only if the person is conscious). Call a physician immediately. 4.2 Most important symptoms and effects, both acute and delayed Symptoms : No information available. 4.3 Indication of any immediate medical attention and special treatment needed Treatment : Treat symptomatically. SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing media : Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. 5.2 Special hazards arising from the substance or mixture Specific hazards during firefighting : No information available. 5.3 Advice for firefighters Special protective equipment for firefighters Further information : In the event of fire, wear self-contained breathing apparatus. : No information available. SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Personal precautions : In the event of an accidental release the emergency response team must respond based on a risk assessment and use personal protective equipment as appropriate. Evacuate personnel to safe areas. 3 / 11