Evidence-based Guidelines for Microbial Source Tracking Projects. September 5th, nd Annual Conference of INAFSM Evansville, IN

Transcription

1 Evidence-based Guidelines for Microbial Source Tracking Projects September 5th, nd Annual Conference of INAFSM Evansville, IN

2 Providing Genetic & Analytic Solutions for Water Digital PCR Pathogens (BSL2) Accredited* Water DNA Lab *World s only ISO Accredited MST Lab Project & Site Analytics Nutrient Source Tracking Host Fecal Score 2

3 QUIT Q U I T Question? Uninterested at this time Information, please. Time to Talk! 3

4 Increasing pressure to get it right 4

5 Available Tools - Legacy Testing (Culture FIB) Concerns: Ineffective at discriminating between sources. 5

6 Available Tools - Field Obs ervations Concerns: Circumstantial and subjective evidence. Difficult to defend. 6

7 Consequence Hinders source abatement Source identification must precede mitigation Weakens the chain of inference Not all sources present the same level of human health ris k Non-fecal < fecal Non-human < human 7

8 DNA-bas ed Microbial Source Tracking 8

9 Example Datasets: Questions Answered Monitoring Receiving Waters for Human Monitoring Outfall Discharges Monitoring Receiving Waters for Multiple Sources Advance 9

10 Monitoring Receiving Waters Locating Sources Aging infrastructure in urban watershed High concentrations of E. coli Human sources expected from leaking sewers Animal sources also suspected What segment would you prioritize? Human-Associated DNA Site Copies/100ml A 9480 B C 479 D E Blank Detected Not Detected 10

11 Monitoring Receiving Waters Locating Sources Bracketed drainage areas Upstream and Downstream comparisons Human-Associated DNA Copies/100ml Site Upstream Downstream Delta A decrease B Detected increase C decrease D increase E Detected Detected none Blank Not Detected NA Menu 11

12 Monitoring Outfall Discharges Locating Sources Combined Storm Sewer with know CSOs E. coli concentrations measured at outfalls Location All Wet Dry A B C D E F G NA H Which outfall would you prioritize? 12

13 Monitoring Outfall Discharges Locating Sources Human-associated DNA measured simultaneously Ranked on E. coli Concentrations Location All Wet Dry A B C D E F G 1 1 NA H Ranked on Human DNA Detections Location All Wet Dry A B C D E F G 1 2 NA H Which outfall would you prioritize? Menu 13

14 Monitoring Receiving Waters for Multiple Sources 4 sampling events in 7 streams (June to October) Stream E. coli Exceedances Goose Dog Human A 3 0 4* 4 B * C D 3* E F G *Highest Concentration Measured What remediation would you propose? Menu 14

15 Designing a Project MST is being applied at each stage of the CWA 15

16 Sampling and Testing Plan ~$250-$800/sample 16

17 Case Studies: Outcomes Achieved MS4 IDDE Evaluation BMP/Remediation Planning BMP Effectiveness Monitoring Natural Source Exclusion Advance 17

18 Where is the pollution coming from - Boston Water and Sewer First ever effectiveness assessment of MS4 IDDE program using DNA markers Human markers measured at outfalls regardless of degree of IDDE completion, and conventional tools (test kits) found to be insufficiently sensitive or specific for detecting illicit discharges New IDDE procedures now recommended, including DNA markers to improve program effectiveness Outcome will be greater bacteria and phosphorus reduction (at lower unit cost and greater health benefit than Green Infrastructure), moving City closer to TMDL compliance Project recognized with national O&M Performance Award from NACWA Menu 18

19 Site Prioritization - Florida DEP/Martin County Menu 19

20 Demonstrating BMP effectiveness - Santa Barbara Menu 20

21 Natural Source Exclusion central Florida Water, sediment and vegetation samples Fecal validation samples Stream removed from 303d Menu 21

22 Microbial Source Tracking - Maturity Precedent Credible Tests Projects in >40 States National Validation (SIPP) Access to Technology Objective Interpretation Laboratories (Accredited) Host Fecal Score 22

23 MST s in stormwater permitting GA NPDES Industrial Storm Water General Permits 23

24 Project Level Probabilistic Modeling 24

25 Microbial Source Tracking Resources Source: Orin Shanks, Biological and Microbial Aspects of Septic System Pollution webinar, June 30, 2015 California Microbial Source Identification Manual Report on state-of-thepractice on source tracking techniques and strategies 25

26 Thank You James Herrin Project Manager Q U I T Question? Uninterested at this time Information, please. Time to Talk! 26

27 Next Steps - Automation 27