Workshop Hydrological Op2cs : Measurements and Modelling

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1 Workshop Hydrological Op2cs : Measurements and Modelling Convidado Externo Emmanuel Boss University of Maine Projeto: FAPESP 2015/ Organizado pelo Laboratório de Instrumentação de Sistemas Aquá2cos (LabISA) INPE/OBT 19 e 20 de abril de às 18:00 horas Auditório do Ins:tuto Interamericano para Pesquisas em Mudanças Globais - IAI Auditório José Simeão de Medeiros LabGeo Sala 27 - Prédio Asa Par:cipantes Ins2tuto Nacional de Pesquisas Espaciais - INPE São José dos Campos - SP Apoio Financeiro

2 Particle size distribution of inland cyanobacteria: Preliminary analysis INPE Na(onal Ins(tute for Space Research Felipe de Lucia Lobo - Post Doc. Jr (CNPq) Evlyn Novo Cláudio Barbosa Lino Sander de Carvalho Daniel Schaffer FURG Federal University of Rio Grande João Sarkis Yunes - Pablo Santos Guimarães Edi Morales Pinheiro Júnior

3 Introduc:on Par:cle Size Distribu:on of phytoplankton is important: Behaviour, movement Grazzing, Ecology, Strategy Species iden:fica:on (size and shape) Spikes and peaks can be associated with groups/species need valida:on. Ahn e Grant (2007) show high correla:on between PSD- LISST and PSD- op:cal micrography. Karp- Boss et al. (2007) evaluates the cell shape effect on the PSD measured by LISST and microscopy.

4 Introduc:on PSD provides informa:on about the Volume Sca`ering Func2on (VSF) which is key for bio- op:cal modelling Remote Sensing Applica2on

5 Volume Sca`ering Func2on (VSF) Introduc:on Input data for Bio- op:cal models Hydrolight Adapted from Daniel, 2014

6 For example, Introduc:on

7 Introduc:on To inves:gate the importance of phytoplankton PSD on VSF, LabISA (INPE) purchased one PSD meter: LISST- Portable that measures PSD of each sample. Improve Bio- op:cal models aiming algae bloom monitoring.

8 Objec:ves The goal is to characterize the PSD of three cyanobacteria species cul:vated in laboratory (at FURG). The ul:mate goal is to provide VSF derived from PSD and simulate its consequences to modeled AOPs (such as Rrs).

9 Specific Objec:ves Measure PSD of three cyanobacteria: Microcys;s aeruginosa, Anabaena sp., e Synechocys;s sp. Validate PSD derived from LISST with PSD derived from microscopy analysis. Evaluate PSD as indicator (proxy) of cyanobacteria species.. If possible, derive VSF from PSD and use them as input data in Hydrolight simula:ons (R rs ).

10 Material & Methods Cynobacteria in vitro Microcys;s sp., individual colonial, mucilaginous; Anabaena sp., filamentous, aerotopes (buoying); Synechocys;s sp., small individual cells

11 Material & Methods LISST portable Light beam at 670 nm through the water sample. Measures volume scapering in 32 bins (0-20 ). Size interval ( µm). Output: Par:cle Volume Distribu:on (µl/l)...?? PSD (freq.) or VSF??

12 Material & Methods VSF Phase func:on, sr - 1 LISST??? ECO- BB9 117 Agrawal et al. (2005) ! Algorithm models from Boss etal.? 2.??

13 Material & Methods # total cells / ml N = n*a/a*1/v n = cells counts A = glass microscope slide area= 4.33 cm 2 a = 10 photos area= cm 2 V= glass slide volume = 2 ml Biovolume (µl/l) Biovolume = N * CV *1000 N = # of cells in 1 ml CV = cell volume (µl) Wetzel & Likens, 2000 Hillebrand et al., 1999

14 Results Synechocys;s sp., small individual cells Microcys;s sp., individual colonial, mucilaginous; Anabaena sp., filamentous, aerotopes (buoying);

15 Results - Synechocys:s LISST 35% 30% 25% 20% 15% 10% 5% 0% 30% 25% 20% 15% 10% 5% 0% Synechocys2s (max. conc.) Total Part. Conc.= µl/l 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns Chl- a = µg/l Synechocys2s (max. conc.) Total Part. Conc.= µl/l Chl- a = µg/l 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns REPLICA 25% 20% 15% 10% 5% 0% 25% 20% 15% 10% 5% 0% Synechocys2s (66% conc.) Total Part. Conc.= 87.8 µl/l (82%) Chl- a = ug/l (55%) 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns Synechocys2s (66% conc.) Total Part. Conc = 97.5 µl/l (75%) Chl- a = µg/l (49%) MICROSCOPY 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns

16 Results - Synechocys:s Photo 400x Dividing

17 10 Photos 400x Resultados - Synechocys:s Cells/ml 1E+7 1E+6 1E+5 1E+4 1E+3 1E+2 1E+1 1E+0 Synechocys:s S. duplicando detritos Biovolume Synechocys:s Diameter = 2.2 μm Vol. Synechocys;s = 4/3*π * r 3 = 14.4 μm 3 Vol. S. duplicando = x 1.5 = 21.2 μm 3 biovolume (µl/l) Synechocys:s S. duplicando LISST Vol. Conc = 97.5 µl/l Chl- a = µg/l

18 Results Synechocys;s sp., small individual cells Microcys;s sp., individual colonial, mucilaginous; Anabaena sp., filamentous, aerotopes (buoying);

19 Resultados - Microcys:s LISST 10% 8% 6% Microcys2s (max. conc.) Total Part. Conc.= µl/l Chl- a = 74.5 µg/l 10% 8% 6% Microcys2s (66% conc.) Total Part. Conc.= 61.7 µl/l (52%) Chl- a = 27.8 µg/l (38%) 4% 4% 2% 2% 0% 10% 8% 6% 4% 2% 0% 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns Microcys2s (max. conc.) Total Part. Conc.= µl/l Chl- a = 75.8 µg/l 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns REPLICA 0% 10% 8% 6% 4% 2% 0% 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns Microcys2s (66% conc.) Total Part. Conc = 90.4 µl/l (77%) Chl- a = 41.1 µg/l (54%) 2,7 3,8 5,3 7,3 10,2 14,2 19,8 27,6 38,4 53,5 74,5 103,7 144,4 201,1 280,1 390,0 Diameter in microns MICROSCOPY

20 Results - Microcys:s Photo 400x Dividing

21 10 Photos 400x Resultados - Microcys:s Cells/ml 1E+6 1E+4 1E+2 Cryptomonadas uni. (4 um) Microcys:s uni. (6um) M. duplicando (9um) detritos 1E+0 Biovolume Cryptomonadas diameter = 4.3 μm Microcys:s Diameter = 6.2 μm Vol. Cryptomonadas = 4/3*π * r 3 = 55.7 μm 3 Vol. Microcys;s = 4/3*π * r 3 = 91.4 μm 3 0 Biovolume (µl/l) Vol. Conc = 61.7 µl/l Chl- a = 27.8 µg/l Cryptomonadas uni. (4 um) Microcys:s uni. (6um) M. duplicando (9um) LISST Vol. M. duplicando = x 1.5 = μm 3 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1

22 Results Synechocys;s sp., small individual cells Microcys;s sp., individual colonial, mucilaginous; Anabaena sp., filamentous, aerotopes (buoying);

23 Results - Anabaena LISST 10% 8% 6% Anabaena (max. conc.) Total Part. Conc.= µl/l Chl- a = - 10% 8% 6% Anabaena (66% conc.) Total Part. Conc.= ul/l (72%) Chl- a = - 4% 4% 2% 2% 0% 2,1 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1 Diameter in microns 0% 2,1 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1 Diameter in microns 10% 8% 6% 4% Anabaena (max. conc.) Total Part. Conc.= µl/l Chl- a = µg/l RÉPLICA 12% 10% 8% 6% 4% Anabaena (66% conc.) Total Part. Conc = µl/l (73%) Chl- a = µg/l (56%) MICROSCOPY 2% 2% 0% 2,1 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1 Diameter in microns 0% 2,1 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1 Diameter in microns

24 Results - Anabaena Photo 400x Zoom das celulas duplicando e cryptomonadas

25 10 Photos 400x Results - Anabaena Par:cles/ml 1E+5 1E+4 1E+3 Chlamydomonas + unicelular + cistos Filamentos detritos 1E+2 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1 Biovolume Chlamydomonas diameter = 4.0 μm Anabaena (one cell) Diameter = 3.1 μm Vol. Chlamydomonas = 4/3*π * r 3 = 33.8 μm 3 Vol. Anabaena = 4/3*π * r 3 = 20.4 μm 3 Vol. Filaments = Vol Anabaena * # cells Biovolume (ul/l) Vol. Conc = µl/l Chl- a = µg/l Chlamydomonas + unicelular + cistos Filamentos LISST 2,9 4,0 5,6 7,8 10,9 15,2 21,1 29,5 41,1 57,3 79,9 111,4 155,4 216,7 302,1

26 Discussion Evaluate into what extend non- linear PSD (opposed to Junge) can affect VSF. Es:mate the contribui:on of different species PSD to VSF Limita:on to 20 degree! Use VSF for different cyanobacteria as input Hydrolight to model AOPs (such as Rrs). Is that possible??