RWP-001. eca biomed. Jul-17 eca biomed GmbH & Co. KG Riedstadt 1

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1 RWP-001 Jul-17 GmbH & Co. KG Riedstadt 1

2 ANOSAN - general properties very active against bacteria, virus and moulds no gaps in effectiveness and no development of microbial resistance known non-toxic to humans, animals and plants; ph-neutral allergic reactions not known no dangerous good, simple handling contains no organic chemicals, produced from water and salt ANOSAN has a shelf life from 6 to 24 months no problematic residues, no special disposal Jul-17 2

3 ANOSAN - microbiocidal effectiveness very active against all micro-organisms bacteria >log5-reduction virus (enveloped and non-enveloped) >log4- reduction Candida and other yeasts >log4-reduction no special disposal Jul-17 3

4 ANOSAN TW first choice for water disinfection no operational interruption of the water supply system immediate removal of micro-organisms from the running water removal of biofilm/ scaling from inner pipe walls during permanent use thus sustainable sanitation of water pipe systems is ensured fulfills the EU/German criteria for drinking water and cooling tower disinfectants purity requirements according to EN DIN 901 production process according to Technical Rule W229 of DVGW effective against Legionella according to EN DIN effective in cold and hot water systems does not increase the corrosion potential of water December-14 no21 4

5 ANOSAN TW - how to use it Buildings (apartment houses, hotels, office blocks, shopping malls): A special water meter is placed downstream of the service water connection. For each volume of 10 liters the water meter transmits an electrical pulse to the pulse receiver of the dosing pump. The dosing pump is fitted with a supply tank equipped with level sensors. With each pulse the pump injects a preset volume of ANOSAN into the running water. The displacement volume of the dosing pump is variable so that the desired concentration of ANOSAN can be adjusted (usually between 0.1 and 1.0%). December-14 5

6 ANOSAN - removal of biofilm Test design Biofilm was grown in silicon tubing by cycling a suspension of the bacteria Pseudomonas aeruginosa in an appropriate nutrient solution for 6 months. For testing the tubing was cut into defined pieces which were incubated in roller flasks. Nutrient solutions without (control) and complemented with disinfectant were replaced every day. The number of colony forming units (cfu) is related to the mass of biofilm. Bevor adding disinfectants the counts were 10 6 to 10 7 cfu per cm² of inner tubing surface. Semi-logarithmic plot. December-14 6

7 ANOSAN - results removal of biofilm comparison with chlorine dioxid Decomposition of biofilm according to Otte, Gebel, Exner; Hyg Med cfu/cm² ClO2 0,2 mg/l control Anolyt 1:400 Anolyt 1:200 mass of biofilm 1,E+08 1,E+07 1,E+06 1,E+05 1,E+04 1,E+03 1,E+02 chlorine dioxid 0.2 ppm control 0,25% Note: Removal of biofilm is a precondition for the sustainable sanitation of all water circuits! 1,E+01 0,5% 1,E days December-14 7

8 Why is ANOSAN unique? Because ANOSAN is a very strong disinfectant 3 drops of ANOSAN can disinfect one litre of water ANOSAN works human identical! ANOSAN has a 2 year shelf life! ANOSAN can be used in a lot of aspects

9 the solution volumetric dosing easy to install easy to use remove reliable bacteria and virus it works odorless and tastless destroy biofilm in waterpipelines no chemicals inexpensive to buy cheap in the application Jul-17 9

10 ANOSAN - effective and safe For the disinfection of one liter of drinking water you need 3 drops only. In contrast to other disinfectants ANOSAN is not a hazardous good, is eco-friendly and non-toxic to humans, animals, and plants and is not irritating. Substitution of unsafe agents helps nature. February 2015 no.23 10