Centre for Sustainable Aquatic Research (CSAR) Craig Pooley College of Science Swansea University

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1 Centre for Sustainable Aquatic Research (CSAR) Craig Pooley College of Science Swansea University PHYCONET Annual Conference 2016

2 About CSAR Founded in 2003 with support from the European Union, Welsh Government and Swansea University. Created with sustainability as a core principle in order to: Develop and transfer sustainable technologies through research Create opportunities to link industry with research expertise Provide training and educational opportunities Equipped with modern, fully programmable recirculating aquaculture systems. CSAR is designed for applied research using a diverse range of aquatic organisms from temperate to tropical and marine to freshwater environments.

3 Facilities CSAR specifications 750m 2 controlled environment building Freshwater to full-strength seawater Ozone sterilised water intake and discharge Air and water temperature control: 9-27 C Programmable lighting light intensity, photoperiod Restricted access to all areas All life support systems monitored and alarmed Full electrical backup via 500 kva generator

4 Facilities Two industry grade Recirculating Aquaculture Systems (RAS) Capacity = 60 m 3 - water circulation rate to 100 m 3 /h 5% system volume exchange per day and maintain 15kg/m 3 of biomass 1.5 & 3.6 m 3 rearing tanks equipped with swirl separators for uneaten feed and faeces collection Oxygen, ozone, temperature & ph control and monitoring

5 Tropical Laboratory Self-contained RAS = 16 x 300L rearing tanks & 18 x 30L trial aquaria Freshwater lab Flow-through or RAS Zebrafish facility Killifish facility Pilot-commercial scale live feeds production 1000L/day microalgae 12 x 800L rotifer tanks 6 x 800L Artemia tanks 6 x 1,200L copepod tanks Primary quarantine laboratory 5 x 1.4m diameter rearing tanks (1.5 m 3 operating volume) Self contained RAS, fully isolated from all other systems

6 Projects EURO-BASIN and imarnet Placing mixotrophs in context All for Water SHOAL SEACAMS FishScan NEPHROPS UK Ocean Acidification Research Programme DeammRecirc

7 Projects Energetic Algae ACCOMPLISH BioAlgaeSorb ShellPlant Algal Biotechnology for Advanced Bio-Products MacroBioCrude MARIBE Project Algal Biotech KTC

8 Microalgae The largest University algal research facility in UK Master culture collection >25 fresh & saltwater species Production lab cultures of 500ml 20L 80L

9 Microalgae 400L Photo-Bioreactor (Biofence TM) 2 x 800L Photo-Bioreactor (Biofence TM) 1,500L tubular Photo-Bioreactor Micro & Ultra Filtration Rigs (>600L/day) Portable algal growth lab

10 Macroalgae Biomass production for biofuel and food Saccharina latissima, Palmaria palmata, Laminaria digitata, Alaria esculenta, Ulva sp. and Porphyra sp. Optimise production for cultivation gametophyte stage

11 Macroalgae Modified nutrient regime to optimise production of gametophytes Increase carbohydrate content - stress PhD Identifying optimum harvest window of Porphyra for increased yield without impacting the wild population. Cultivate in the lab and at pilot scale. Macroalgae testing facilities in Pembrokeshire (2 double longlines)

12 Analytical tools Cell count, biovolume (Multisizer 4, Beckman, USA) C:N analyser Water chemistry by automated segmented flow analyzer (AA3, Bran Luebbe, Germany) HPLC GC Biochemical composition: Lipids (Bligh and Dyer, 1959 ) Carbohydrates (DuBois et al. 1956) Pigments (Griffiths et al. 2011) FTIR Spectroscopy Biochemical fingerprinting Photoefficiency PHYTO-PAM (PHYTO-ED, Walz, Germany)

13 Education Undergraduate Single & Joint Honours degrees Post graduate studies PhD, MRes and MSc Training Aquaculture Water Quality Control Recirculating Aquaculture Systems (RAS) Marine Hatchery Technologies Biosecurity, Hygiene and Health Management Aquaculture Nutrition and Feeds Aquaculture Waste Management Algal Culturing and Maintenance Publications >100 ISI papers

14 MANY THANKS