Research projects on insects at NIFES

Transcription

1 Research projects on insects at NIFES AquaFly, ENTOFÔR, NICE Erik-Jan Lock

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3 Nutrients Contaminants Pathogens Fish health and welfare Nutrients Contaminants Fillet quality

4 Nutrients Contaminants Pathogens Insect health and welfare Nutrients Contaminants Product quality

5 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality

6 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality

7 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality

8 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality

9 Red algae Bangiales Gigartinales Palmariales -Porphyra umbilicalis -Porphyra dioica -Porphyra purpurea -Chondrus crispus -Mastocarpus stellatus -Furcellaria lumbricalis -Palmaria palmata Bodø Ulvales -Ulva lactuca -Ulva intestinalis Green algae Cladophorales -Cladophora rupestis Brown algae Fucales Laminariales -Fucus serratus -Fucus vesiculosus -Fucus spiralis -Ascophyllum nodosum -Pelvetia canaliculata -Halidrys siliquosa -Himanthalia elongata -Saccharina latissimi -Alaria esculenta -Laminaria digitate Ectocarpales -Chordaria flagelliformis

10 Porphyra cf. purpurea Porphyra dioica Porphyra cf. Umbilicalis Palmaria palmata Hierarchical cluster analysis Unsupervised principal component analysis Bangiales and Palmariales species exhibited similar metabolite profiles in comparison to profiles from other red algae

11 Chordaria flagelliformis Alaria esculenta Saccharina latissima Laminaria digitata Hierarchical cluster analysis Unsupervised principal component analysis Metabolites of the algal species belongig to the laminariales and ectocarpales order were grouped separately

12 % o f d ry w e ig h t 2 5 P r o t e in P o r p h y r a c f. p u r p u r e a P o r p h y r a c f. le u c o s tic ta U lv a c f. in te s tin a lis U lv a s p. (b la d e d ) P o r p h y r a s p. P o r p h y r a c f. p u r p u r e a C la d o p h o r a r u p e s tr is U lv a c f. in te s tin a lis C h o n d r u s c r is p u s P a lm a r ia p a lm a ta P o r p h y r a c f. u m b ilic a lis A la r ia e s c u le n ta M a s to c a r p u s s te lla tu s S a c c h a r in a la tis s im a F u r c e lla r ia lu m b r ic a lis C h o r d a r ia fla g e llifo r m is? L a m in a r ia h y p e r b o r e a C h o r d a r ia fla g e llifo r m is? H im a n th a lia e lo n g a ta H a lid r y s s iliq u o s a F u c u s v e s ic u lo s u s P e lv e tia c a n a lic u la ta F u c u s s p ir a lis F u c u s s e r r a tu s F u c u s v e s ic u lo s u s F u c u s s p ir a lis A s c o p h y llu m n o d o s u m A s c o p h y llu m n o d o s u m e c a d. m a c k a y i A s c o p h y llu m n o d o s u m

13 % o f d ry w e ig h t 1 5 F a t 1 0 A s c o p h y llu m n o d o s u m A s c o p h y llu m n o d o s u m e c a d. m a c k a y i A s c o p h y llu m n o d o s u m 5 0 F u c u s s p ir a lis F u c u s s p ir a lis P e lv e tia c a n a lic u la ta F u c u s v e s ic u lo s u s H a lid r y s s iliq u o s a F u c u s v e s ic u lo s u s C h o r d a r ia fla g e llifo r m is? C h o r d a r ia fla g e llifo r m is? F u c u s s e r r a tu s P o r p h y r a c f. p u r p u r e a S a c c h a r in a la tis s im a U lv a c f. in te s tin a lis P o r p h y r a c f. p u r p u r e a P a lm a r ia p a lm a ta L a m in a r ia h y p e r b o r e a C la d o p h o r a r u p e s tr is U lv a s p. (b la d e d ) A la r ia e s c u le n ta H im a n th a lia e lo n g a ta P o r p h y r a c f. u m b ilic a lis C h o n d r u s c r is p u s U lv a c f. in te s tin a lis P o r p h y r a s p. F u r c e lla r ia lu m b r ic a lis M a s to c a r p u s s te lla tu s

14 A r s e n ic C a d m iu m B r o w n L.d ig ita t a B r o w n G r e e n 4 0 m g kg -1 m a x a llo w e d G r e e n 1 m g kg -1 m a x a llo w e d fo r fe e d m a te ria ls o f v e g e ta b le o rig in fo r s e a w e e d m e a l R e d R e d m g k g -1 d w m g k g -1 d w

15 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality Feasible Seaweed (%) Control (%) Feeding group S0 S10 S20 S30 S40 S50 S60 S70 S80 S90 S100

16 BSF composition Protein content % DM in all groups Amino acid profile does not change in the larvae fed seaweed enriched media Lipid content changes:

17 Eicosapentaenoic acid EPA ~1 % EPA

18 Iodine

19 Feed-food safety

20 Undesirable elements

21 Undesirable elements

22 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality Two feeding trials: - Freshwater salmon (2016) - EWOS - 8 weeks - Start 50 gr End 130 gr - Seawater salmon (2017) - GIFAS - 6 months - Start 1 kg 4 kg

23 Freshwater feeding trial EWOS Fish meal SPC Diet 1 Diet 2 Diet 3 Protein Insect meal Diet 4 Diet 5 Diet 6 Fish oil Insect lipid Protix Lipid Insect lipid AquaFly

24 Formulation and proximate composition of the experimental diets CTL FM/IL FM/ILAF IM/FO IM/IL IM/ILAF FM LT IM VPC Binder FO Veg Oil Insect Oil Aquafly Oil Vit&Min premix Misc Sum % 12% 12% 60% 65% 65% FA (mg g -1 ) CTL FM/IL FM/ILAF IM/FO IM/IL IM/ILAF 12:0 < : : Saturated FA Sum MUFA Sum n Sum n Sum PUFA

25 F is h g a in Growth performances S F G C R R C tl F M /IL F M /IL A F IM /F O IM /IL IM /IL A F The lipid sources had a significant effect on fish gain and specific growth rate, no effect of protein source

26 H S I F I Growth performances F C R V S I C tl 8 0 F M /IL F M /IL A F IM /F O IM /IL IM /IL A F

27 Sustainability evaluation (social, environmental and economic) and ethical considerations Nutrients, contaminants, pathogens Fish health and welfare Nutrients Contaminants Fillet quality - Seawater salmon (2017) - GIFAS - 6 months - Start 1 kg 4 kg Insect lipid Insect meal

28 Insects in the Circular Economy S G R % E P A in in s e c t Underused (marine) resources Insects convert carbohydrate-rich organic material into animal protein and lipid One third of the world s food is wasted 1.3 billion tons X X insectmeal 1.5 insectlipid E P A % E P A in g ro w th m e d ia Retain valuable nutrients in the food chain and increase protein supply in a sustainable manner Specific growth rate Cloying Earthy Aftertaste Flavour/taste FM100 A100 B25 Sour 9 8 Salt Bitter Metallic Fillet quality Food safety Sensory testing Fish meal Insect meal Chemical Marine Rancid Fish-oil Vegetable-oil

29 ENTOFÔR from waste to resource NorInsect AS Myldregard AS Botngaard Bioprotix AS

30 Main aim To deliver knowledge and tools that policy makers, companies and other stakeholders need to direct and develop a waste-fed insect industry for feed production in Norway

31 WP 1: Identify attractive Norwegian organic waste streams that may be converted by insects to produce raw materials for animal feed (WP1 leader: Ivar Pettersen, NIBIO) Task 1.1: Identify factors determining suitability of organic waste as insect feed Task 1.2. Segmented study of waste streams for insect production

32 WP 2: Evaluate and establish insect species cultures and methods that are relevant to convert the suggested waste streams into protein feed sources (WP2 leader: Ingeborg Klingen, NIBIO) Task 2.1: Determine insect species and establish methods for insect cultures on suggested organic waste streams Task 2.2: Test relevant waste streams as insect feed - Insect feeding studies

33 Work package 3: Development of insect functional ingredients (WP3 leader: Vincenzo Fogliano, WUR) Task 3.1: Development and characterization of protein based insect ingredients - Cost-effective isolation and fractionation of insect protein - Digestibility test in fish Task 3.2: Development and characterization of lipid based insect ingredient - Optimization of lipid extraction methods - Test in aquafeed

34 WP 4. Develop methods for detection and tracing of non-legal waste use in the insect production chain (WP leader: Marc Berntssen NIFES) Task 4.1.: Peptidomic profiling of different insect species reared on specific waste products Task 4.2.: Quantification of specific waste products or insect species in a feed ingredient mixture

35 WP 5: Validate and perform a wide scope screening for pesticides and mycotoxins for insect reared on different waste products (WP leader: Marc Berntssen, NIFES) Task 5.1. Development and validation of a wide scope screening method for detection of pesticides and mycotoxins in insect feed and insect products Task 5.2. Screening of pesticides and mycotoxins in several waste substrates and insect materials

36 WP6 Communication with stakeholders and dissemination of results (WP leader: Erik-Jan Lock, NIFES) Task 6.1 Participation and communication with stakeholders and end-users Task 6.2 Project website with project activities and results Task 6.3: Project management

37 NICE aims to form a knowledge base that can support sustainable development of the insect industry in Norway and the Nordic region. 1. Organizing seminars in Norway, Sweden, Finland and Denmark 2. Website: insect-network.org (.com)

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41 Thank you for your attention!