CERTIFICATION. Certificate No. The AOAC Research Institute hereby certifies that the performance of the test kit known as: MC Media Pad ACplus

Transcription

1 CERTIFICATION AOAC Pefomance Teted SM Cetificate No The AOAC Reeach Intitute heeby cetifie that the pefomance of the tet kit known a: MC Media Pad ACplu manufactued by JNC Copotation 2 1, Otemachi 2 chome Chiyoda ku Tokyo Japan Thi method ha been evaluated in the AOAC Pefomance Teted Method SM Pogam, and found to pefom a tated by the manufactue contingent to the comment contained in the manucipt. Thi cetificate mean that an AOAC Cetification Mak Licene Ageement ha been executed which authoize the manufactue to diplay the AOAC Pefomance Teted SM cetification mak along with the tatement "THIS METHOD'S PERFORMANCE WAS REVIEWED BY AOAC RESEARCH INSTITUTE AND WAS FOUND TO PERFORM TO THE MANUFACTURER'S SPECIFICATIONS" on the above mentioned method fo a peiod of one calenda yea fom the date of thi cetificate (Decembe 19, 2017 Decembe 31, 2018). Renewal may be ganted at the end of one yea unde the ule tated in the licening ageement. Deboah McKenzie Deboah McKenzie, Senio Diecto Signatue fo AOAC Reeach Intitute Decembe 19, 2017 Date 2275 Reeach Blvd., Ste. 300, Rockville, Mayland, USA Telephone: Fax: Intenet e mail: aoaci@aoac.og * Wold Wide Web Site:

2 METHOD AUTHORS Hajime Teamua and Gail Bett SUBMITTING COMPANY JNC Copoation Yokohama Reeach Cente 5 1, Ookawa, Kanazawa ku, Kanagawa Japan KIT NAME(S) MC Media Pad ACplu CATALOG NUMBERS JNC Cop: SK01A25, SK01A10, SK91B10 INDEPENDENT LABORATORY Campden BRI Ltd. Station Rd Chipping Campden, Gloucetehie GL55 6LD, UK AOAC EXPERTS AND PEER REVIEWERS Yi Chen 1, Michael Bodky 2, Yvonne Salfinge 3 1 FDA CFSAN, College Pak, MD, USA 2 Bodky Conultant, Ontaio, Canada 3 Conultant, Denve, CO APPLICABILITY OF METHOD Taget oganim Total Aeobic Count Matice (MLG 3.02) beat (50 g), Raw gound pok (50 g) (SMEDP Chapte 6) Ceam cheee (11 g), Yogut dink (11 ml) (OMA ) Paley (50 g), Vegetable juice (50 ml), Cooked pawn (50 g), (50 g), Egg alad andwich (50 g), Deli pata alad with feta (50 g) (ISO 4833:2013) beat (10 g), Raw gound pok (10 g), Ceam cheee (10 g), Yogut dink (10 ml), Paley (10 g), Vegetable juice (10 ml), Cooked pawn (10 g), (10 g), Egg alad andwich (10 g), Deli pata alad with feta (10 g) REFERENCE METHODS U.S. Depatment of Agicultue, Food Safety and Inpection Sevice (2015) Micobiology Laboatoy Guidebook, Chapte 3.02, Quantitative Analyi of Bacteia in Food a Sanitay Indicato, af b 718d6592bcc9/MLG3.pdf?MOD=AJPERES (7) Standad Method fo the Examination of Daiy Poduct (2004) 17 th Edition, APHA Pe, Wahington, DC, Chapte 6, Micobiological Count Method, (8) Official Method of Analyi (2016) 20 th Edition, AOAC INTERNATIONAL, Gaithebug, MD, Method , (9) ISO :2013 Micobiology of the food chain Hoizontal method fo the enumeation of micooganim Pat 1: Colony count at 30 degee C by the pou plate technique, (11) Pefomance claim Pefomance equivalent to that of the U.S. Depatment of Agicultue Food Safety and Inpection Sevice Micobiology Laboatoy Guidebook (USDA FSIS/MLG) 3.02 Quantitative Analyi of Bacteia in Food a Sanitay Indicato fo 50 g tet potion of aw gound pok and aw chicken beat unde tandad and apid uage condition; the Standad Method fo the Examination of Daiy Poduct (SMEDP) Chapte 6 Micobiological Count Method, Standad Plate Count Method, fo 11 ml tet potion of yogut dink unde tandad and apid uage condition and 11 g tet potion of ceam cheee unde tandad uage condition; AOAC Official Method Micobiological Method fo 50 g tet potion of paley, vegetable juice, tuna pate, and pata alad unde tandad and apid uage condition and 50 g tet potion of pawn and andwiche unde tandad uage condition; and ISO :2013 Micobiology of the food chain Hoizontal method fo the enumeation of micooganim Pat 1: Colony count at 30 degee C by the pou plate technique fo 10 g tet potion of all claimed matixe unde altenate method condition. ORIGINAL CERTIFICATION DATE Septembe 05, 2017 METHOD MODIFICATION RECORD None Unde thi AOAC Pefomance Teted SM Licene Numbe, thi method i ditibuted by: 1. Meck KGaA CERTIFICATION RENEWAL RECORD Renewed and appoved though 2018 SUMMARY OF MODIFICATION None Unde thi AOAC Pefomance Teted SM Licene Numbe, thi method i ditibuted a: 1. MC Media Pad Rapid Aeobic Count with Meck (Millipoe Sigma) catalog numbe

3 PRINCIPLE OF THE METHOD (1) The MC Media Pad ACplu i a tet pad coated with gowth medium and thee edox indicato fo detection of aeobic bacteial colonie. Once the liquid ample i inoculated onto the tet pad, the ample diffue though the whole pad by capillay action, econtituting the medium. If aeobic bacteia ae peent, they gow a ed coloed colonie on the tet pad. Fo mot food, a 50 g o 50 ml tet potion i homogenized with 450 ml Buttefield Phophate Buffe. Seial decimal dilution ae pepaed a needed. Fo daiy poduct, an 11 g tet potion of ceam cheee i added to 99 ml 2% odium citate wamed to C and homogenized. An 11 ml tet potion of yogut dink i diluted into 99 ml Buttefield phophate buffe. Seial dilution of daiy poduct ae pepaed with 1 ml o 11 ml of homogenate diluted into 99 ml Buttefield phophate buffe. A 1 ml aliquot of diluted ample i applied to the tet pad, the cove film i eplaced, and the plate incubated at 35±1 C fo 24±2 h (apid uage) o 48±2 h (tandad uage). Even though the apid uage can be ued fo poceed food etc. afte veified by ue, it doe not apply to food containing lage amount of lactic acid bacteia o pychophilic bacteia. Altenatively, a 10 g tet potion of matix i diluted into 90 ml Maximum Recovey Diluent (MRD). Seial dilution ae pepaed with 10 ml homogenate diluted into 90 ml MRD. A 1 ml aliquot of diluted ample i applied to the tet pad, the cove film i eplaced, and the plate incubated at 30±1 C fo 72±3 h (altenate method). DISCUSSION OF THE VALIDATION STUDY (1) The MC Media Pad ACplu method pefomed a well o bette than the elevant efeence method teted in the matix tudy. In all matixe teted, thee wa le than a 0.5 log 10 cfu/g diffeence between the candidate and efeence method and the epeatability of the method wa vey imila. The eult howed that the altenative method wa vey veatile in it application. It howed compaable pefomance to the USA efeence method unde tandad uage (48±2 h at 35±1 C) fo all 10 matixe and unde apid uage (24±2 h at 35±1 C) fo 7 matixe. In addition it howed compaable eult to the ISO efeence method (72±3 h at 30±1 C) fo all 10 matixe. The eult of thi validation tudy indicate that the MC Media Pad ACplu method can be ued fo apid and accuate enumeation of total aeobic count in a vaiety of food and unde a vaiety of tet condition. Table 8. Matix Study Reult fo Standad Uage (35 C, 48 h) (1) LCL UCL Low Raw gound pok Med High Low Med beat High Low Feh paley Med High Low Vegetable juice Med High Low Cooked pawn Med High Low Med High Low Sandwiche Med High Low Pata alad Med High Low Yogut dink Med High Low Ceam cheee Med High N: numbe of eplicate S: Standad deviation of eplicate LCL: Lowe confidence limit UCL: Uppe confidence limit

4 Table 10. Matix Study Reult fo Rapid Uage (35 C, 24 h) (1) LCL UCL Low Raw gound pok Med High Low Med beat High Low Feh paley Med High Low Vegetable juice Med High Low Med High Low Pata alad Med High Low Yogut dink Med High N: numbe of eplicate S: Standad deviation of eplicate LCL: Lowe confidence limit UCL: Uppe confidence limit

5 Table 12. Matix Study Reult fo Altenate Uage (30 C, 72 h) (1) LCL UCL Low Raw gound pok Med High Low Med beat High Low Feh paley Med High Low Vegetable juice Med High Low Cooked pawn Med High Low Med High Low Sandwiche Med High Low Pata alad Med High Low Yogut dink Med High Low Ceam cheee Med High N: numbe of eplicate S: Standad deviation of eplicate LCL: Lowe confidence limit UCL: Uppe confidence limit REFERENCES CITED 1. Teamua, H. and Bett, G., Evaluation of the MC Media Pad ACplu fo Enumeation of Aeobic Count in a Vaiety of Food, AOAC Pefomance Teted SM cetification numbe Appoved Septembe AOAC Reeach Intitute Validation Outline fo MC Media Pad ACplu. 3. AOAC INTERNATIONAL Method Committee Guideline fo Validation of Micobiological Method fo Food and Envionmental Suface (2016) Official Method of Analyi of AOAC INTERNATIONAL, 20th Ed., Appendix J, AOAC INTERNATIONAL, Gaithebug, MD, acceed 7/20/ U.S. Depatment of Agicultue, Food Safety and Inpection Sevice (2015) Micobiology Laboatoy Guidebook, Chapte 3.02, Quantitative Analyi of Bacteia in Food a Sanitay Indicato, af b 718d6592bcc9/MLG3.pdf?MOD=AJPERES, acceed 7/20/ Standad Method fo the Examination of Daiy Poduct (2004) 17 th Edition, APHA Pe, Wahington, DC, Chapte 6, Micobiological Count Method, acceed 7/20/ Official Method of Analyi (2016) 20 th Edition, AOAC INTERNATIONAL, Gaithebug, MD, Method , acceed 7/20/ Lukaz Komta (2011) Tet fo outlie: R package veion poject.og/package=outlie, acceed 7/20/ ISO :2013 Micobiology of the food chain Hoizontal method fo the enumeation of micooganim Pat 1: Colony count at 30 degee C by the pou plate technique, acceed 7/20/17.