ROU Information Sheet No Sampling and Sample Management for Consistent Analysis of Recycled Organics Products. Sampling and Sample Management

Transcription

1 ROU Infrmatin Sheet N Recycled Organics Unit PO Bx 6267 University f New Suth Wales Sydney, NSW 1466 Phne a.campbell@recycledrganics.cm Sampling and Sample Management fr Cnsistent Analysis f Recycled Organics Prducts The Australian Standard AS4454 (2012) Cmpsts, sil cnditiners and Mulches includes a new Appendix A: Sampling, Sample Handling and Preparatin which prvides general guidance n sampling, and specific guidance n sample quantities (derived frm internatinal standards). Hwever, Appendix N: Maturity Index in the standard prvides specific guidance n sample handling and preparatin fr bilgical stability tests, withut which test results will be unreliable. Neither appendix prvides infrmatin in a frm that can be directly implemented. This ROU infrmatin sheet prvides principles and peratinal practice fr sampling, sample handling and shipping methds that are cnsistent with the requirements f AS4454 (2012) standard. These methds are cnsistent with the requirements fr sampling, handling and analysis f samples fr the respirmetry test ptins specified in the Maturity Index f the revised AS4454 (2012) standard fr the assessment f bilgical stability (O2 uptake, CO2 evlutin, NDI, Slvita ). Sectin 1 Sampling and Sample Management 1.1 Purpse f sampling The first step in analyzing cmpst quality is t btain a representative sample frm the cmpst pile. The sample (and sampling pints) shuld reflect the verall r average characteristics f the material being tested. Due t natural variatin in raw materials and in envirnmental cnditins within the cmpst pile (albeit within an expected range) prperly representative sample must be frmed by mixing numerus incremental samples t frm a cmpsite sample fr analysis. Labratry testing is expensive. Tests perfrmed n a sample that is nt representative f the bulk f material, r that has nt been crrectly handled will prduce unreliable results that may misrepresent the characteristics f the cmpst batch as a whle. This is undesirable fr manufacturer s quality cntrl purpses and fr generating prduct infrmatin; and fr selectin and use by custmers. 1.2 Definitins Batch: A quantity f gds manufactured frm knwn materials by the same prcess under the same cnditins and assumed t have the same characteristics. The surce/s f supply and perid ver which the materials have been received fr an individual batch shuld be knwn fr traceability. Incremental sample: a discrete quantity f material taken frm ne discrete sampling pint. Cmbined r cmpsite sample: cmbinatin f mixed the incremental samples frm a single batch. Sampling site/sampling area: an area f a cmpst windrw r pile that is deliberately prepared t expse an internal crss sectin f the pile frm which incremental samples can be taken. 1.3 Safety / disclaimer Care shuld be taken when handling waste materials and samples that may cntain sharps and sharp fragments, chemical cntaminants r pssible pathgenic rganisms. Facility peratrs shuld ensure that sampling and assciated peratins are carried ut in a safe manner that prtects staff and minimises risks. Such risks are dependent upn engineering arrangement and equipment used at the facility, and shuld be addressed via risk assessment by managers f the facility. Recycled Organics Unit Cmpst Sampling and Sample Management Page 1

2 1.4 Sample management fr maturity test accuracy Cmpst maturity is nw assessed in AS4454 (2012) by assessing bth the bilgical stability f a prduct, and als cmplementary assessment f plant grwth respnse attributes relevant t the relative presence/absence f phyttxic characteristics (see AS4454 Appendix N). Bilgical stability can be defined as the extent t which readily bidegradable material has decmpsed. A material is cnsidered unstable if it cntains a high prprtin f bidegradable matter that can sustain high micrbial activity. If the material cntains mainly recalcitrant r humuslike matter, it is nt able t sustain high levels f micrbial activity under suitable envirnmental cnditins (temperature and misture), and therefre it is cnsidered bilgically stable. Respirmetry test methds assesses the level f bilgical activity in a sample f material under specified cnditins that are cnducive t micrbial activity by measuring the rate f respiratin in the frm f carbn dixide evlved r xygen cnsumed by micrrganisms, r in the frm f the heat generated by this bilgical activity. Respiratin is directly related t the metablic activity f a micrbial ppulatin, the micr-rganism ppulatin will cllectively respire at higher rates in the presence f higher levels f bilgically available rganic matter, while micrbilgical activity and ttal respiratin will be lwer where such material is scarce (under equivalent cnditins). Tests specify the quantity f suitably mist cmpst that is incubated at a specified temperature under specific cnditins fr measurement. Respirmetry tests can prvide a repeatable and quantitative r semi quantitative measure f the degree t which a material has been decmpsed and stabilized. Respiratin determinatin is reprted t be sensitive t the time lag between sample cllectin and analysis. T minimize changes in the sample due t micrbial activity ver time, samples shuld be analysed as sn as pssible after cllectin. Fr reliability f results, ideally samples shuld be prepared and assessment begun within 48 hurs f sampling. This requires prir arrangement with the labratry s that they are expecting the sample t arrive and have the test apparatus available t cnduct the test in a timely manner. Temperature and misture cntent during sample transprt, handling and preparatin are widely reprted thrughut the literature as critical fr reliability f respiratin test indices as bilgical activity is a functin f bth parameters. Adequate sample misture is critical. Test methds specify sample preparatin requirements including misture and temperature adjustment, and lag time fr micrbial acclimatisatin. Acclimatisatin is particularly imprtant where samples have dried t < 40% misture, r where samples have been enclsed in a sealed cntainer withut adequate air fr an extended perid f time, r have been frzen during transprt. Samples fr micrbial testing must nt be frzen. T minimize changes in the sample due t micrbial activity with samples maintained at temperatures f >1 C and <4 C during handling and transprt; and packaged and handled in a manner t best avid risk f freezing, high temperatures, drying ut, and the develpment anaerbic cnditins. The methds described aim fr increased validity and cnsistency in labratry testing by ensuring a representative sample is btained and by minimising risk f damaging impact n samples in transprt. There are knwn interferences that can distrt respirmetry test results, including the fllwing: a) Time lag between sampling and testing: respiratin determinatin is reprted t be sensitive t the time lag between sample cllectin and analysis. T minimize changes in the sample due t micrbial activity ver time, samples shuld be analysed as sn as pssible after cllectin. Ideally samples shuld be prepared and assessment begun within 48 h f sampling. If this is nt pssible, a sample management plan shuld be agreed with the labratry cnducting the test. b) Temperature and misture cntent during sample transprt, handling and preparatin are widely reprted thrughut the literature as critical parameters fr respiratin test indices, as bilgical activity is a functin f bth temperature and suitable misture cntent. T minimize changes in the sample due t micrbial activity, samples shuld be maintained at temperatures f Recycled Organics Unit Cmpst Sampling and Sample Management Page 2

3 >1 C and <4 C during handling and transprt, and packaged and handled in a manner that best avids bth drying ut and the nset anaerbic cnditins. Test methds specify sample preparatin requirements, including misture and temperature adjustment, and lag time fr micrbial acclimatizatin. Acclimatizatin is particularly imprtant where samples have dried t < 35% misture, have been enclsed in a sealed cntainer withut adequate air fr an extended perid f time, r have been frzen during transprt. c) Vlatile ammnia can be a significant cause f nxius durs and is knwn t be phyttxic and t inhibit micrbial activity at elevated levels. Trials and labratry experience have shwn false psitive results frm respirmetry-based tests due t the suppressin f bilgical activity frm elevated vlatile ammnia levels. Vlatile ammnia assessment shuld be implemented t infrm the validity f results frm respirmetry tests. d) Particle size fr test: respirmetry tests fr cmpsts in UK, EU and USA standards are cmmnly cnducted n cmpst prducts f 10 mm particle size grade. As requirements in Table N3.2 are based n internatinal standards and research, it is recmmended that respirmetry assessments fr cmpliance with this Standard be carried ut n a 10 mm particle size fractin f the specimen fr test. e) Acclimatizatin and rewetting f dry samples: where the received sample is assessed t have a misture cntent belw 40%, the sample shuld be remistened and thrughly mixed t a misture cntent f 50%. T acclimatize micrbial activity prir t testing, place the remistened sample in an unsealed cntainer that avids cntaminatin, is ut f direct sunlight and is in a draught-free envirnment at a temperature f (25 C ±2 C) fr 48 hurs prir t cnducting tests specified fr bilgical stability. NOTE: this requirement applies irrespective f any ther requirement specified in an individual methd. Labratry tests where respiratin is calculated per quantity f vlatile slids f) Carbnates can increase vlatile slids determinatins due t the release f CO2 during sample cmbustin at 550 C in the LOI (Lss n Ignitin) methd specified fr BVS. Samples with significant carbnate cntent must be pre-treated (leached with acid) prir t analysis t remve carbnates. g) Inert materials, including petrleum-based materials such as film and hard plastics can increase vlatile slids determinatins due t the release f CO2 during sample cmbustin at 550 C in the LOI methd specified fr BVS, therefre plastics and ther cntaminants shuld be remved t the greatest extent pssible prir t cmbustin. The mass f cntaminants remved prir t sample cmbustin shuld be recrded and test results adjusted t accunt fr the mass f inert materials in the riginal sample. 1.5 Wrking with yur lab Sample management practices shuld be agreed with the labratry cnducting the test, and shuld be dcumented n the purchase rder. In additin, the labratry shuld be advised upn dispatch f the sample frm the facility and arrangements made fr the labratry t cnfirm receipt f sample. One advantage f the current AS4454 (2012) standard is that simple tests are nw available fr n-site assessment f prduct maturity. This includes TMECC 05-05B biassay, and the Slvita maturity index test kit (which prvides tw tests, assessing bth CO2 evlutin and als vlatile ammnia). This allws manufacturers t readily test prducts prir t sale fr cmpliance with maturity specificatins. T best crrelate the results with full characterisatin test cnducted by yur labratry service prvider, check and ensure the temperature settings in yur n-site lab rm are equivalent t thse f yur independent labratry service prvider fr mre cmparable bilgical stability results; and make sure yu are using the same seed variety and cnditins as yur lab fr the biassay. Recycled Organics Unit Cmpst Sampling and Sample Management Page 3

4 1.6 Determining the number f incremental samples required per batch The number f sampling pints required t btain a representative sample f cmpst frm a batch is calculated frm internatinal standards 1, nte that these quantities A minimum f 12 and a maximum f 30 incremental samples shall be btained frm a cmpst batch f defined vlume (f up t 5,000 m 3 ), then thrughly mixed int a representative cmpsite sample: Number f incremental samples required fr piles f different size Windrw r pile vlume N. f increment samples required 575 m m m m m m m m 3 30 The internatinal standards fr sampling frm which these calculatins are btained is relevant fr sampling materials where the prperties being tested fr are cnsistently distributed thrughut the material. The AS4454 (2012) standard prvides guidance fr the minimum required size f each incremental sample n the basis f the maximum particle size range f the material being sampled, as shwn in the table belw. The bjective is t ensure the sampling methd is nt biased twards aviding larger particles: Minimum incremental sample size fr materials f different particle size Largest size mm Litres per increment sample Nte that all incremental samples shuld all be f a cnsistent size. Sampling peratins shall be carried ut ver a sufficiently shrt perid f time and in such a way as t avid any alteratin in the characteristics f the prduct. During sampling, all incremental samples shall be handled and stred in a manner that avids cntaminatin and maintains their characteristics. 1 British Standards Institutin. (2000). British Standard BS EN 12579:2000 Sil imprvers and grwing media: Sampling. British Standards Institutin (BSI), Lndn, United Kingdm This is the English language versin f the DIN EN : 1999 Eurpean Standard. Recycled Organics Unit Cmpst Sampling and Sample Management Page 4

5 1.7 Sample divisin methd: cning and quartering The result f cllecting and mixing the specified number f incremental samples each f cnsistent vlume f the specified minimum required size may result in a cmpsite sample f significantly larger quantity than is required fr testing. After thrugh mixing, the resulting representative cmpsite sample (r cmbined sample) can be divided int representative sub-samples via the cning and quartering methd Apparatus All apparatus must be clean 20 L plastic pails with lids; Spade/s; Plastic sheet (use the thickest available plyethylene builders plastic); Means f cleaning the apparatus Methd cning and quartering a) Empty the increment samples nt a clean plastic sheet t avid cntaminating the sample with material frm the grund. Using a clean spade, arrange int a cnical pile and thrughly mix by wrking the pile whilst mving thrugh tw full circuits arund the pile. b) Cut the pile int 4 segments f equal size using the spade (as thugh cutting slices f a cake), and pull the segments apart with the blade t separate frm each ther; c) Take tw equally sized ppsite segments and cmbine them t frm a representative sub sample (final sample) that is apprximately half f the cmpsite sample in vlume. This cmbined final sample fr test is then packaged and transprted t the labratry. d) Take the ther tw equally ppsite sized segments and cmbine them and retain this cmpsite final sample (apprximately half f the cmpsite sample in vlume) at the facility as an archive sample. This is imprtant in case the final sample sent t the labratry is lst in transit r the labratry experiences difficulties, r if re-testing f the sample is required fr any reasn. If pssible it is preferable t refrigerate the archive sample in a suitably labelled and sealed cntainer (ideally less than 4 C but nt belw 1 C); therwise stre the cntainer in a dark, dry, cl lcatin ut f direct sunlight at a temperature f less than 10 C but nt belw 0 C t reduce any change in characteristics ver time. e) Cning and quartering can be repeated sequentially t halve the sample again as required t further reduce the cmpsite sample int smaller representative final samples f suitable quantity. f) Discard the unneeded r remaining material, r therwise add back t a new batch f incming raw materials fr reprcessing. CONING AND QUARTERING METHOD: Dividing a representative cmpsite sample int 2 representative sub-samples = lab sample = archive sample Recycled Organics Unit Cmpst Sampling and Sample Management Page 5

6 Sectin 2 Windrw sampling methds and diagrams 2.1 Methd A: sampling cmpletely mixed cmpst piles Apparatus All apparatus must be clean 20 L plastic pails with lids. Trwels r trenching shvel. Means f cleaning the apparatus. Suitable means f turning the cmpst pile Sampling methd A Arrange fr the pile t be turned t break up clumps and cmpletely mix and hmgenize the materials. Fr a pile that has been s thrughly mixed with clumps brken up and distributed with a windrw turner (r equivalent), increment samples f cnsistent size can be taken frm randm psitins (sampling sites) frm alng the exterir f the length and height f the pile immediately after mixing. Ideally sample within 2 hurs f mixing as a range f characteristics such as misture distributin, O2 availability and bilgical distributin can alter rapidly, and certainly within a few hurs f mixing. Use a clean trenching shvel r trwel t extract incremental samples f cnsistent vlume at varius, evenly spaced lcatins frm the sides f the windrw. Dig a plug frm the pile fr each incremental sample rather than simply scaping frm the surface. During sampling take care t handle and stre incremental samples in a manner that maintains their characteristics, being careful t avid crss cntaminatin f the sampling sites prir t sampling (eg with turner wheels r mechanism), and f the increment samples during sampling and mixing (eg. frm windblwn materials). Avid sampling frm the ends f the windrw where cnditins are unique (in terms f surface area t vlume rati) and are nt representative f cnditins in the greater cmpsting mass. Recycled Organics Unit Cmpst Sampling and Sample Management Page 6

7 Methd A: cmpst sampling diagram Fr a batch that has been thrughly mixed with a windrw turner (r equivalent) in a manner that breaks up and distributes clumps, incremental samples f cnsistent size can be taken frm the exterir f the pile immediately after mixing, taking samples frm each side alng the length f the windrw. Each incremental sample represents a different sampling site. Dig a plug frm the pile fr each incremental sample rather than simply scaping frm the surface. Avid sampling frm the ends f a windrw as cnditins there are unique and nt representative f the greater cmpsting mass. The number f incremental samples required fr a repre- Windrw sentative cmpsite sample depends n the size f the cmpst pile. Recycled Organics Unit (2011) Incremental samples Cmpsite r cmbined sample Thrughly mix incremental samples frm multiple sampling sites int a representative cmpsite sample. Use a clean trenching shvel r trwel t extract incremental samples f cnsistent and adequate vlume, taking care t handle and stre samples in a manner that maintains their characteristics, and being careful t avid crss cntaminatin f the sampling sites prir t sampling (eg with turners); and f the increment samples during sampling (eg. frm windblwn materials). Recycled Organics Unit Cmpst Sampling and Sample Management Page 7

8 2.2 Methd B: Sampling cmpst piles withut cmplete mixing Apparatus All apparatus must be clean 20 L plastic pails with lids. Trwels r trenching shvel. Means f cleaning the apparatus. Suitable means f turning the cmpst pile Methd B: sampling cmpst piles withut adequate mixing A different sampling methd is required where the windrw r pile is unturned, r turning methds d NOT cmpletely break up and distribute clumps and hmgenize the materials. Arrange fr a wheel lader r excavatr t dig r back-blade half way in t a sectin f the pile expsing a near vertical crss sectin face (alternatively, fr smaller piles, a lader may cut a crss sectin thrugh the entire windrw). This vertical face represents a sampling site that allws increment samples t be extracted frm the crss sectin f the windrw. Sampling shuld ccur immediately after pening and expsing the sampling site with the machinery (ideally within 2 hurs as a range f characteristics such as misture distributin, O2 availability and bilgical distributin can alter rapidly, and certainly within a few hurs f mixing). Use the clean trenching shvel r trwel t extract between three and five increment samples f cnsistent vlume at varius, evenly spaced lcatins frm the expsed crss sectin face f the windrw. Use a clean trenching shvel r trwel t extract increment samples f cnsistent vlume at varius, evenly spaced lcatins frm the expsed crss sectin faces f the windrw. Dig a plug frm the pile fr each incremental sample rather than simply scaping frm the surface. During sampling take care t handle and stre incremental samples in a manner that maintains their characteristics, being careful t avid crss cntaminatin f the sampling sites prir t sampling (eg with turners), and f the increment samples (eg. frm windblwn materials). Nte that samples are extracted frm the crss sectin face, and NOT the centre line face within the windrw (as this wuld ver represent material frm the centre f the pile). Avid sampling frm the uter mm layer when sampling (tp, bttm and sides), and avid sampling frm the ends f the windrw where cnditins are unique (in terms f surface area t vlume rati) and are nt representative f cnditins in the greater cmpsting mass. Recycled Organics Unit Cmpst Sampling and Sample Management Page 8

9 Methd B: Windrw cmpst sampling diagram Fr use where a windrw r pile is unturned, r turning methds d NOT cmpletely hmgenize the materials. Use a lader r excavatr t dig half way int the pile t expse a near vertical crss sectin face, which becmes a sampling site. A number f incremental samples f cnsistent quantity can be taken frm each expsed crss sectin face f the windrw, representing the distributin f materials and cnditins thrughut the mass. D nt sample frm the expsed centre line face as this wuld ver represent material in the centre f the pile. Avid sampling frm the uter mm r the ends f a windrw as cnditins are unique and nt representative f the greater mass. The number f sampling sites and incremental samples required fr a representative cmpsite sample f the windrw depends n the size f the cmpst pile. Incremental samples Centre line Recycled Organics Unit (2011) Cmpsite r cmbined sample Thrughly mix incremental samples frm multiple sampling sites int a representative cmpsite sample. Use a clean trenching shvel r trwel t extract increment samples f cnsistent vlume, taking care t handle and stre samples in a manner that maintains their characteristics, and being careful t avid crss cntaminatin f the sampling sites prir t sampling (eg with lader r excavatr), and f the increment samples (eg. frm windblwn materials) Methd C: sampling cmpst piles assessment f beneficial micrrganisms Nte, this is nt relevant fr pathgen r indicatr pathgen assessment, which must be cnducted n a representative sample btained in accrdance with methds abve. In the instance that a custmer requests an assessment f the micrbial cmmunities f a cmpst fr identificatin f micrrganisms that are beneficial t sil health, such as cunts f ttal r active fungal and/r bacterial cmmunities: a) Samples shuld preferably be taken frm lcatins in the pile that mst clsely represent envirnmental cnditins (misture, air, heat) that will ccur when the cmpst has been applied. This will better represent the micrbial activity that the cmpst is able t supprt nce applied. b) There is little value in assessing cmpst sampled frm lcatins in the pile where envirnmental cnditins are irrelevant t applicatin cnditins and may be entirely unsuitable fr the rganisms being tested fr. c) Generally fllw methd B abve, sampling nly frm areas f the crss sectin face f the cmpst pile that best represent relevant envirnmental cnditins. Recycled Organics Unit Cmpst Sampling and Sample Management Page 9

10 2.3 Packaging and shipping The cmpsite sample fr testing by the labratry must be packaged in such a way that characteristics are unaltered n arrival at the labratry, and must be clearly labelled fr identificatin and t maintain chain f custdy. Arrangements fr testing shuld be made with the labratry prir t sampling and shipping, including written cnfirmatin f the analysis t be cnducted, hw the sample is t be managed n arrival, and expected date f sample delivery (t ensure test apparatus is available). Use a 10 litre sealable cntainer (a rbust plastic handy pail with sealable lid is suitable where samples are sent and received n the same day by rad transprt. Where samples are sent by air r vernight transprt a fully enclsed EPS (plystyrene) cntainer with tight sealing lid that can be securely taped t seal is preferred t avid extremes f temperature. A small EPS esky is suitable fr small samples, r a fully enclsed ice pack brccli EPS vegetable bx fr larger samples. Lad sample int strng high density plyethylene sampling bags then place bags int the cntainer. Where cntainers have been previusly used, thrughly clean the cntainers 2, and rinse 3 times with clean water drain and air dry prir t lading the cmbined sample (with clean shvel) directly frm the cning and quartering prcess abve. Fr new cntainers, clean and then rinse with clean water prir t use. Securely attach shipping labels with tape t the utside f the cntainer (1 n tp, 1 n side). The label shuld clearly identify the delivery address and cntact details fr the labratry. Place a similar label inside the cntainer, with a letter prviding sample and client details, and specifying the analysis t be cnducted enclsed in a zip-lck plastic lunch bag. Fr chain f custdy, the labels and/r enclsed paperwrk shuld als clearly identify: The surce facility (facility name); A unique batch identificatin number; The type and grade f material; The number f incremental samples cmbined fr this representative labratry sample; The date and time f sampling; The persn respnsible fr sampling and packaging (name and signature); and The facility cntact persn/number. Place pre-frzen, sealed gel type ice-pack/s n tp f the sample in the packaging cntainer (d nt include ice that can melt and leak), then immediately prir t shipping securely install the lid t seal, and tape arund the lid t ensure it cannt be disldged. Fr respirmetry tests, samples shuld be received by the lab and sample preparatin begun within 48 hurs f sampling. During this perid, sme methds specify that samples must nt be frzen, and that temperatures abve 4 C are permitted fr n mre than 24 hurs. Keep the cntainer and sample in a dark, dry, cl lcatin ut f direct sunlight whilst awaiting cllectin (ideally refrigerated between 1 and 4 C, r therwise less than 10 C, but nt frzen). Arrange fr cllectin n the same day f sampling, r the mrning fllwing. Use a delivery service that will deliver it t the labratry within 24 hurs. 2 Where cntainers are simply siled washing and brushing with mild detergent slutin prir t triple rinsing is sufficient. Cleaning slutins such as Decn are suitable fr sterilising cntainers where there is cncern ver the ptential presence f chemical r bilgical residues, see Recycled Organics Unit Cmpst Sampling and Sample Management Page 10

11 Upn dispatch r cllectin by delivery curier, cntact the testing labratry t cnfirm that the sample is in transit and t prvide an ETA fr delivery (eg. tmrrw) Packaging fr analysis f chemical cntaminants Where a sample is t be tested fr ptential presence f chemical cntaminants, ideally pack a prtin f the labratry sample int a glass jar sealed with a metal cap r with aluminium fil under the cap t avid inadvertent secndary cntaminatin as rganic pesticides can migrate thrugh plastic lids. 500 ml is sufficient fr mulch samples and 250 ml fr sil cnditiner samples Shipping label: tp f cntainer: Print and cmplete fr each sample, and attach t sample fr transprt t lab, cnsistent with the packaging and shipping instructins abve. Labratry delivery address: DELIVERY ADDRESS Lab cntact persn: Lab cntact number: Sample fr analysis: Please keep cl and ut f direct sunlight Prject cde: Sample #: Please advise by when sample is received t cnfirm analysis required. Cntact Recycled Organics Unit Cmpst Sampling and Sample Management Page 11

12 2.3.3 Shipping label: side f cntainer: Print and cmplete fr each sample, and attach t the side f the package. Labratry delivery address: DELIVERY ADDRESS Lab cntact persn: Lab cntact number: Sample fr analysis: Please keep cl and ut f direct sunlight Please pen samples when received t allw exchange f air. Sample preparatin and analysis shuld begin within 48 hurs f sampling. Samples shuld be kept cntained in a dark, dry, cl lcatin ut f direct sunlight (ideally less than 10 C but nt belw 1 C. D nt freeze samples). Sample has been shipped n day f cllectin, and shuld arrive at the labratry by curier the day after sampling. Sample details (and chain f custdy): Please advise by when sample is received t cnfirm analysis required. Cntact Prject/batch cde: Sample #: Details t be cmpleted by persn respnsible fr sampling and packaging: Facility name: Batch number: Material type and grade: Sample quantity: Number f incremental samples cmbined in this sample: Sampling date and time: Name: Signature: Recycled Organics Unit Cmpst Sampling and Sample Management Page 12