The objective of this work was the production of ethanol

Size: px
Start display at page:

Download "The objective of this work was the production of ethanol"

Transcription

1 1.12 Bioethanol from sugar cane bagasse Urribarrí, Lauris 1 * Ferrer, Alexis 2 Aiello, Cateryna 3 Rivera, Jhoandry 4 Abstract The objective of this work was the production of ethanol by simultaneous saccharification and fermentation (SSF) from sugar cane bagasse treated with ammonia at 1.25 kg/kg dry matter ammonia loading, 30% moisture content, 100 C for 5 min on the ethanol production by thermotolerant Kluyveromyces marxianus CECT Non treated bagasse was used as a control. The results are compared with separate saccharification and fermentation (SHF). Enzymatic hydrolysis was carried out with 5% w/v dry bagasse using 5 IU/g dm of cellulases (Accellerase 100) at 50 C for 48 h and the fermentation at 42 C for 96 h. The SSF was carried out for 96h at 42 C. The sugars released in the hydrolysis were measured with the 3.5 dinitrosalicylic acid method and the ethanol produced by gas chromatography. The SSF ethanol yields with respect to the theoretical value for the treated material was 71%, 1.85 times higher than that obtained for the untreated material and also considerably higher than SHF (1.64 times higher). Clearly, elimination of feedback inhibition due to sugars by SSF enhanced considerably the sugar yield, and therefore ethanol yield. Sugar cane bagasse can be used for bioetha- 1 Chemistry Department, Science Faculty, University of Zulia, Maracaibo, Venezuela. 2 Zulian Institute of Technological Research, Km. 15 vía La Cañada de Urdaneta, Venezuela. 3 Biochemical Engineering Department, Engineering Faculty, University of Zulia, Maracaibo, Venezuela. 4 Biology Department, Science Faculty, University of Zulia, Maracaibo, Venezuela. * Corresponding author II Congreso Iberoamericano sobre Biorrefinerías

2 nol production with a 71% yield by using a mild ammonia treatment, low enzyme doses and a simultaneous saccharification and fermentation. Keywords: Ammonia treatments, Ethanol production, Sugar cane bagasse. Introduction Lignocellulosic materials are the major source of cellulose and hemicellulose in nature and have been recognized as a potential renewable and inexpensive source of to obtain fermentable sugars for fuel ethanol production (second generation biofuels) and other products of high added value, mainly by biotechnological and / or thermochemical processes. However, the complex organization and interaction of the cell wall polymers make it necessary to subject them to physical and chemical pretreatments [DOE, 2005] which minimize structural barriers, increasing their chemical and enzymatic susceptibility. Ammonia processes are an excellent alternative because the volatile agent is easily recovered and process conditions are relatively mild which ensures a minimum degradation of the biomass (Ferrer et al, 2000). Sugar cane bagasse (SCB) is the third largest agro-industrial residue in Venezuela, with an approximate production of 1,580,000 tons per year (FEDEAGRO, 2010) and barely 50% of the volume produced is used. Its high carbohydrate content makes it an attractive substrate for production of bioethanol. Traditionally, SCB is burned to produce a modest amount of energy and as a way to limit the disposal of this waste. The pretreatment of pressurization and depressurization Ammonia (PDA) (Ferrer et al, 2000) which is a treatment with ammonia in liquid phase under pressure, using relatively low temperatures and short times, is available at a pilot plant scale and a 1 ton/h demonstration plant is currently being built. This treatment has been successful in eliminating structural barriers such as high crystallinity of cellulose, high lignin content, high acetylation of hemicellulose and high degree of linkage between these molecules. The aim of this study was to produce bioethanol from ammonia treated SCB by simultaneous saccharification and fermentation. 96 Methodology Substrate SCB was provided by La Pastora, a mill located in Carora, Lara, Venezuela. The sample was dried in a forced convection oven at 60C for 48 hours to reduce its moisture content to 10%, and stored in plastic bags until use. SCB was sieved to a size not greater than 2 mm. 2 nd Iberoamerican Congress on Biorefineries

3 Ammonia treatments Samples of 350 g of dry substrate were treated with ammonia in a 2 kg pilot plant at 1.25 kg of ammonia / kg dry substrate loading, 30% moisture contents at 100ºC for 5 min, using a pressure of 300 psi (ATB). Subsequently, the treated samples were aerated under a hood for 12 h. Non treated bagasse (NTB) was used as a control. Biomass Analysis The moisture content of the samples was determined by the method described in AOAC (2010) for SCB. The contents of hemicellulose, cellulose and lignin were determined using the method of Goering and Van Soest (1970) based on determinations of neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid lignin (ADL). Separate Saccharification and Fermentation For these experiments, the saccharification of untreated (NTB) and ammonia treated (ATB) sugarcane bagasse was performed using Accellerase and β-glucosidase for 48 h. A 5% substrate and 5 IU cellulase/g dm were used. After this, the hydrolysate was filtered and the fermentation was initiated by adding the inoculum of the organism corresponding to a volume equal to 10% of the workload. The sugars released in the hydrolysis were measured with the 3.5 dinitrosalicylic acid method and the ethanol produced by gas chromatography. Simultaneous Saccharification and Fermentation The process was applied to both NTB and ATB. The substrate, the enzyme complex Accellerase and β-glucosidase, and thermotolerant Kluyveromyces marxianus CECT 10875, were added simultaneously. The SSFs were performed in 500 ml Erlenmeyer flasks with 100 ml of the culture medium at 100 rpm for 72 hours in an orbital incubator model Innova 4300 (New Brunswick Scientific, Edison NJ, USA), in duplicate. A 5% substrate and 5 IU cellulase/g dm were used. The medium was inoculated with a 10% volume of the workload to initiate the fermentation. Aliquots were taken at 0, 6, 12, 24, 48 and 72 hours. Sugars and ethanol were measured as in SHF. Results The compositions of NTB and ATB were 49.32% cellulose, 23.96% hemicellulose, 11.05% lignin, 11.08% solubles, and 36.82% cellu- 97 II Congreso Iberoamericano sobre Biorrefinerías

4 lose, 13.75% hemicellulose, 8.96% lignin, 21.89% solubles, respectively. The treatment solubilized 43% of the hemicellulose and 25% of the cellulose. The enzymatic hydrolysis of NTB and ATB produced 8.5 mg/ ml and mg/ml of reducing sugars, respectively, for 48 h of hydrolysis. Figure 1 shows the fermentation of both NTB and ATB hydrolysates. For NTB, as the fermentation proceeds the concentration of ethanol increases whereas the glucose concentration decreases in the medium, until exhausted at 24 h at which time the fermentation ceases. The ethanol yield with respect to the theoretical value obtained from the fermentation of NTB was 26.30%, which is quite low but expected for a recalcitrant material with a high lignin content and intricate fiber structure, which has not undergone prior treatment. Figure 1 Kinetics of the fermentation using Kluyveromyces marxianus CECT a) Untreated bagasse hydrolysate. b) Treated bagasse hydrolysate 98 For ATB, the system behaved as for NTB, however, glucose was exhausted at 48 h, due to the higher concentration of glucose to start. The total consumption of glucose indicates that the treatment did not produce inhibitory compounds or at least not in concentrations which could inhibit the yeast. The fermentation yield of the ATB hydrolysate was 65% higher than the untreated, however, it is relatively low (43.24%). This suggests the need either to apply more aggressive ammonia treatment conditions, to further simplify the lignocellulosic biomass matrix, or to use SSF. The kinetics of the SSF for the untreated and treated material is shown in Figure 2. During the first phase, there was a steady increase in ethanol concentration while the glucose content remained very low, showing a good performance of the yeast during the fermentation. The glucose concentration increased only slightly at the end of the trials. 2 nd Iberoamerican Congress on Biorefineries

5 The highest concentrations of ethanol for both NTB and ATB were obtained at 96 hours, although 48 and 72 h may be considered as final times, respectively, since the difference between 48 and 96 h for NTB (3.8%) and between 72 and 96 for ATB (5,1%) were small. Figure 2 Kinetics of SSF for SCB. a) Untreated, b) treated The ethanol yield with respect to the theoretical value was 71.29%, 1.64 times higher than in SHF, clearly indicating the importance of removing the glucose as it is formed. The yield is higher than those reported by Bollók et al. (2000) in SSF experiments (44%) with 5% of substrate (softwoods treated with steam explosion) using strains of K. marxianus, In another study (Ballesteros et al., 2004), using the yeast K. marxianus CECT in SSF with a substrate concentration of 10%, yields of ethanol with respect to the theoretical were between 60 and 71%, using different lignocellulosic substrates, results that are consistent with those obtained in this work with the same thermotolerant strain but using only one third of the cellulolytic enzymes and 5% substrate. Ethanol yields obtained for the treated material in this work were between 1.8 and 1.9 times higher than those obtained for the untreated material. Conclusions The ammonia treatment considerably increased the ethanol yields compared to untreated, but most important, the use of SSF conditions improved the ethanol yields compared to those obtained by SHF. In the case of ATB, the increase was 1.64 times. The production of bioetha- 99 II Congreso Iberoamericano sobre Biorrefinerías

6 nol in SSF processes allowed not only to obtain higher yields but also they were achieved in less time, 48h and 72h for the untreated and treated materials, respectively, while the SHF processes for both materials required 24 h more. It is possible to further increase the ethanol yields by optimizing pretreatment and SSF conditions. Acknowledgement Authors thank the contribution of FONACIT (Caracas, Venezuela) and the Swiss Agency for Development and Cooperation (Lausanne, Switzerland) for partly financing this work, and to the CIEMAT (Madrid, Spain) for kindly supplying the yeast strain. References 100 AOAC, Official methods of analysis. (2010). 18th edition online, Association of Official Analytical Chemists. Available in: eoma.aoac.org. Ballesteros, M., Oliva, J., Negro, M., Manzanares, P., Ballesteros, I. (2004). Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SSF) with Kluyveromyces marxianus CECT Process Biochemistry, 39 (12), Bollók, M., Réczey, K., Zacchi, G. (2000). Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol. Applied Biochemistry and Biotechnology, 84/86, DOE, U.S. Department of Energy (2005). Breaking the biological barriers to cellulosic ethanol: a join research agenda. A research road map resulting from the biomass to biofuels workshop, December 7-9, Maryland, USA, 206 p. Ferrer, A., Byers, F. M., Sulbarán de Ferrer, B., Dale, B. E., Aiello, C. (2000). Optimizing ammonia pressurization/depressurization processing conditions to enhance enzymatic susceptibility of dwarf elephant grass. Applied Biochemistry and Biotechnology, 84/86, FEDEAGRO. Confederación Nacional de Asociaciones de Productores Agropecuarios. (2010). Estadísticas Agrícolas. Venezuela. Goering, H., Van Soest, P. (1970). Forage fiber analyses (apparatus, reagents, procedures, and some applications), Agric. Handbook N 379. ARS-USDA, Washington, D.C. 8 p. 2 nd Iberoamerican Congress on Biorefineries

Pretreatment Methods for Banana Peel as a Substrate for the Bioproduction of Ethanol in SHF and SSF

Pretreatment Methods for Banana Peel as a Substrate for the Bioproduction of Ethanol in SHF and SSF Pretreatment Methods for Banana Peel as a Substrate for the Bioproduction of Ethanol in SHF and SSF Nuttiya Chantawongsa Division of Biochemical Technology, School of Bioresources and Technology, King

More information

Summary & Conclusion

Summary & Conclusion Summary & Conclusion CHAPTER 6 SUMMARY & CONCLUSIONS Concerns regarding the soaring cost of gasoline and the depleting petroleum reserves have led to an urge for a sustainable alternative to gasoline such

More information

Optimization of the pretreatment of wheat straw for production of bioethanol

Optimization of the pretreatment of wheat straw for production of bioethanol Optimization of the pretreatment of wheat straw for production of bioethanol Eva-Lena Jakobsson Department of Chemical Engineering, Lund University Abstract Bioethanol has some advantages over petrol as

More information

THERMOPHILIC ENZYMES FOR BIOMASS CONVERSION

THERMOPHILIC ENZYMES FOR BIOMASS CONVERSION Bioenergy- II: Fuels and Chemicals from Renewable Resources THERMOPHILIC ENZYMES FOR BIOMASS CONVERSION Dr. Francesco La Cara Institute of Protein Biochemistry C.N.R. Naples - Italy THERMOPHILIC ENZYMES

More information

Optimization of alkaline peroxide pretreatment of rice straw

Optimization of alkaline peroxide pretreatment of rice straw Optimization of alkaline peroxide pretreatment of rice straw Jaruwan Damaurai 1, Verawat Champreda 2, Navadol Laosiripojana 1* 1. The Joint Graduate School of Energy and Environment, King Mongkut s University

More information

Ethanosolv Pretreatment of Bamboo with Dilute Acid for Efficient Enzymatic Saccharification

Ethanosolv Pretreatment of Bamboo with Dilute Acid for Efficient Enzymatic Saccharification Ethanosolv Pretreatment of Bamboo with Dilute Acid for Efficient Enzymatic Saccharification Zhiqiang LI Ph.D. lizq@icbr.ac.cn 55th International Convention of Society of Wood Science and Technology Main

More information

Improvements in Bioethanol Production Process from Straw

Improvements in Bioethanol Production Process from Straw Improvements in Bioethanol Production Process from Straw Heike Kahr,*, Alexander G. Jäger Upper Austria University of Applied Sciences Research and Development Ltd, Campus Wels Stelzhamerstrasse, A- Wels,

More information

Biofuel production using total sugars from lignocellulosic materials. Diego Alonso Zarrin Fatima Szczepan Bielatowicz Oda Kamilla Eide

Biofuel production using total sugars from lignocellulosic materials. Diego Alonso Zarrin Fatima Szczepan Bielatowicz Oda Kamilla Eide Biofuel production using total sugars from lignocellulosic materials Diego Alonso Zarrin Fatima Szczepan Bielatowicz Oda Kamilla Eide scope of the presentation 1. Available lignocellulosic materials 2.

More information

Enzymatic Conversion of Biomass to Ethanol

Enzymatic Conversion of Biomass to Ethanol Enzymatic Conversion of Biomass to Ethanol Alfalfa/Corn Rotations for Sustainable Cellulosic Biofuels Production June 29-30, 2010 Pioneer Hi-Bred Carver Center Johnston, IA 2 Genencor, a Danisco Division

More information

Effect Of Alkali Pretreatment and Enzymatic Saccharification on Bagasse Reducing Sugar For Bioethanol Production

Effect Of Alkali Pretreatment and Enzymatic Saccharification on Bagasse Reducing Sugar For Bioethanol Production 1). Technical Implementation Unit for Development of Chemical Engineering Processes GunungKidul, Yogyakarta, Indonesia. Effect Of Alkali Pretreatment and Enzymatic Saccharification on Bagasse Reducing

More information

The Biorefinery approach to production of lignocellulosic ethanol and chemicals from lignocellulosic biomass

The Biorefinery approach to production of lignocellulosic ethanol and chemicals from lignocellulosic biomass The Biorefinery approach to production of lignocellulosic ethanol and chemicals from lignocellulosic biomass IEA Bioenergy Conference, Vienna 13-14.11.2012 Gisle L Johansen Senior Vice President R&D and

More information

Conversion of Corn-Kernel Fiber in Conventional Fuel-Ethanol Plants

Conversion of Corn-Kernel Fiber in Conventional Fuel-Ethanol Plants Conversion of Corn-Kernel Fiber in Conventional Fuel-Ethanol Plants Executive Summary National Corn to Ethanol Research Center Ethanol derived from corn-kernel fiber is qualified as cellulosic biofuel

More information

MATERIALS & METHODS Microorganisms and cultivation. Keywords: Corncob; Cellulosic hydrolysates; Streptomyces sp.; Reducing sugar; Bioethanol

MATERIALS & METHODS Microorganisms and cultivation. Keywords: Corncob; Cellulosic hydrolysates; Streptomyces sp.; Reducing sugar; Bioethanol Pretreatment and hydrolysis of cellulosic agricultural wastes with a cellulaseproducing Streptomyces for bioethanol production Chuan-Liang Hsu a, Ku-Shang Chang b, Yi-Huang Chang b, Hung-Der Jang b, *

More information

Novozymes Cellic CTec3 HS - secure your plant's lowest cost

Novozymes Cellic CTec3 HS - secure your plant's lowest cost Bioenergy Application sheet Novozymes Cellic CTec3 HS - secure your plant's lowest cost Cellic CTec3 HS is a highly efficient cellulase and hemicellulase complex that ensures a cost-efficient conversion

More information

Degradation kinetics of steam exploded wheat straw during anaerobic digestion for biogas production

Degradation kinetics of steam exploded wheat straw during anaerobic digestion for biogas production Ref: C0472 Degradation kinetics of steam exploded wheat straw during anaerobic digestion for biogas production Javier Lizasoain, alps Centre for Climate Change Adaptation, Grabenweg 68, 6020 Innsbruck,

More information

Steam Pretreatment Optimisation for Sugarcane Bagasse in Bioethanol Production

Steam Pretreatment Optimisation for Sugarcane Bagasse in Bioethanol Production Steam Pretreatment Optimisation for Sugarcane Bagasse in Bioethanol Production Johan Sendelius Master of Science Thesis 2005 Department of Chemical Engineering, Lund University, Sweden Abstract: Steam

More information

Nordic Association of Agricultural Scientists

Nordic Association of Agricultural Scientists NJF Report Vol 3 No 4 Year 2007 Nordic Association of Agricultural Scientists NJF Seminar 405 Production and Utilization of Crops for Energy Vilnius, Lithuania, 25-26 September 2007 Straw biomass potential

More information

RESEARCH PAPERS FACULTY OF MATERIALS SCIENCE AND TECHNOLOGY IN TRNAVA SLOVAK UNIVERSITY OF TECHNOLOGY IN BRATISLAVA

RESEARCH PAPERS FACULTY OF MATERIALS SCIENCE AND TECHNOLOGY IN TRNAVA SLOVAK UNIVERSITY OF TECHNOLOGY IN BRATISLAVA RESEARCH PAPERS FACULTY OF MATERIALS SCIENCE AND TECHNOLOGY IN TRNAVA SLOVAK UNIVERSITY OF TECHNOLOGY IN BRATISLAVA 2011 Number 31 OZONE PRETREATMENT OF WHEAT STRAW AND ITS EFFECT ON REDUCING SUGARS IN

More information

2G ethanol from the whole sugarcane lignocellulosic biomass

2G ethanol from the whole sugarcane lignocellulosic biomass Pereira et al. Biotechnology for Biofuels (21) 8:44 DOI 1.1186/s1368-1-224- RESEARCH ARTICLE 2G ethanol from the whole sugarcane lignocellulosic biomass Open Access Sandra Cerqueira Pereira 1, Larissa

More information

The effect of acid pretreatment on bio-ethanol and bio-hydrogen production from sunflower straw

The effect of acid pretreatment on bio-ethanol and bio-hydrogen production from sunflower straw nd International Conference on Sustainable Solid Waste Management The effect of acid pretreatment on bio-ethanol and bio-hydrogen production from sunflower straw G. Antonopoulou 1, G. Dimitrellos 1, D.

More information

Influence of harvesting time on biochemical composition and glucose yield from hemp

Influence of harvesting time on biochemical composition and glucose yield from hemp Agronomy Research 11 (1), 215 220, 2013 Influence of harvesting time on biochemical composition and glucose yield from hemp M. Tutt *, T. Kikas and J. Olt Institute of Technology, Estonian University of

More information

Biofuels Research at the University of Washington

Biofuels Research at the University of Washington Biofuels Research at the University of Washington 15 July 2008 Rick Gustafson Paper Science & Engineering College of Forest Resource University of Washington UW biofuels research agenda Vision: Cost effective

More information

2.2 Conversion Platforms

2.2 Conversion Platforms 2.2 Conversion Platforms The strategic goal of the conversion element is to develop technologies for converting feedstocks into cost-competitive commodity liquid fuels, like ethanol, as well as bioproducts

More information

Effects of Liquid Hot Water Pretreatment on Enzyme Loading and Hydrolysis of Hardwood

Effects of Liquid Hot Water Pretreatment on Enzyme Loading and Hydrolysis of Hardwood 1 Effects of Liquid Hot Water Pretreatment on Enzyme Loading and Hydrolysis of Hardwood Michael Ladisch, Youngmi Kim, Ja Kyong Ko, Tommy Kreke, Eduardo Ximenes Laboratory of Renewable Resources Engineering

More information

Effect of particle size on enzymatic hydrolysis of pretreated miscanthus

Effect of particle size on enzymatic hydrolysis of pretreated miscanthus Engineering Conferences International ECI Digital Archives BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals Proceedings Spring 6-13-2013 Effect of particle size on enzymatic

More information

COMPARISON OF DIFFERENT PRETREATMENT METHODS ON DEGRADATION OF RYE STRAW

COMPARISON OF DIFFERENT PRETREATMENT METHODS ON DEGRADATION OF RYE STRAW COMPARISON OF DIFFERENT PRETREATMENT METHODS ON DEGRADATION OF RYE STRAW Marti Tutt, Timo Kikas, Juri Olt Estonian University of Life Sciences marti.tutt@emu.ee Abstract. This article investigates the

More information

SECOND GENERATION BIOETHANOL FROM Eucalyptus globulus labill AND Nothofagus pumilio USING IONIC LIQUIDS. María Cristina Ravanal E.

SECOND GENERATION BIOETHANOL FROM Eucalyptus globulus labill AND Nothofagus pumilio USING IONIC LIQUIDS. María Cristina Ravanal E. SECOND GENERATION BIOETHANOL FROM Eucalyptus globulus labill AND Nothofagus pumilio USING IONIC LIQUIDS. María Cristina Ravanal E. Centro de Biotecnología y Bioingeniería Universidad de Chile mravanal@ing.uchile.cl

More information

Production of Fermentable Sugars from Recycled Paper Sludge for Alcohol Production

Production of Fermentable Sugars from Recycled Paper Sludge for Alcohol Production Production of Fermentable Sugars from Recycled Paper Sludge for Alcohol Production Nantanat Kulsuwan Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University

More information

Developing Herbaceous Energy Crops

Developing Herbaceous Energy Crops USDA - ARS - National Center for Agricultural Utilization Research Developing Herbaceous Energy Crops Michael A. Cotta Fermentation Biotechnology Research Current Paradigm Corn Wheat Sugarcane Potential

More information

BIOETHANOL PRODUCTION FROM CELLULOSIC FIBERS: COMPARISON BETWEEN BATCH AND FED-BATCH SACCHARIFICATION

BIOETHANOL PRODUCTION FROM CELLULOSIC FIBERS: COMPARISON BETWEEN BATCH AND FED-BATCH SACCHARIFICATION BIOETHANOL PRODUCTION FROM CELLULOSIC FIBERS: COMPARISON BETWEEN BATCH AND FED-BATCH SACCHARIFICATION CÁTIA V. T. MENDES, M. GRAÇA V. S. CARVALHO and JORGE M. S. ROCHA CIEPQPF, Department of Chemical Engineering,

More information

Fermentation of pretreated source separated organic (SSO) waste for ethanol production by different bacteria

Fermentation of pretreated source separated organic (SSO) waste for ethanol production by different bacteria Fermentation of pretreated source separated organic (SSO) waste for ethanol production by different bacteria by Bekmuradov Valeriy, Luk Grace and Luong Robin Ryerson University Toronto, Canada Montreal,

More information

BIOETHANOL PRODUCTION FROM CELLULOSIC FIBERS: COMPARISON BETWEEN BATCH AND FED-BATCH SACCHARIFICATION

BIOETHANOL PRODUCTION FROM CELLULOSIC FIBERS: COMPARISON BETWEEN BATCH AND FED-BATCH SACCHARIFICATION CELLULOSE CHEMISTRY AND TECHNOLOGY BIOETHANOL PRODUCTION FROM CELLULOSIC FIBERS: COMPARISON BETWEEN BATCH AND FED-BATCH SACCHARIFICATION CÁTIA V. T. MENDES, M. GRAÇA V. S. CARVALHO and JORGE M. S. ROCHA

More information

Cellulosic Biomass Chemical Pretreatment Technologies

Cellulosic Biomass Chemical Pretreatment Technologies Life-changing Research and Development Cellulosic Biomass Chemical Pretreatment Technologies September 6, 2007 Keith Pauley Keith.Pauley@matricresearch.com 800-611-2296 Chemical and Environmental Technologies

More information

Alkali pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for production of ethanol

Alkali pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for production of ethanol ISBN 978-979-18962-0-7 Alkali pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for production of ethanol Yanni Sudiyani*, Syarifah Alawiyah, Kiky C. Sembiring Research Center

More information

SWEET SORGHUM JUICE AND BAGASSE AS A POSSIBLE FEEDSTOCK FOR BIOETHANOL PRODUCTION

SWEET SORGHUM JUICE AND BAGASSE AS A POSSIBLE FEEDSTOCK FOR BIOETHANOL PRODUCTION HUNGARIAN JOURNAL OF INDUSTRIAL CHEMISTRY VESZPRÉM Vol. 36(1-2) pp. 43-48 (28) SWEET SORGHUM JUICE AND BAGASSE AS A POSSIBLE FEEDSTOCK FOR BIOETHANOL PRODUCTION M. GYALAI-KORPOS 1, J. FECZÁK 2, K. RÉCZEY

More information

Ethanol Production from Biomass - Optimization of Simultaneous Saccharification and Fermentation with Respect to Stirring and Heating

Ethanol Production from Biomass - Optimization of Simultaneous Saccharification and Fermentation with Respect to Stirring and Heating Ethanol Production from Biomass - Optimization of Simultaneous Saccharification and Fermentation with Respect to Stirring and Heating JESPER NÖRGÅRD Department. of Chemical Engineering, Lund Institute

More information

AN ENZYMATIC PROCESS OF BIOETHANOL PRODUCTION USING AGRICULTURAL WASTES BY Saccharomyces cerevisiae (MTCC 173) AND Zymomonas mobilis (MTCC 2427)

AN ENZYMATIC PROCESS OF BIOETHANOL PRODUCTION USING AGRICULTURAL WASTES BY Saccharomyces cerevisiae (MTCC 173) AND Zymomonas mobilis (MTCC 2427) AN ENZYMATIC PROCESS OF BIOETHANOL PRODUCTION USING AGRICULTURAL WASTES BY Saccharomyces cerevisiae (MTCC 173) AND Zymomonas mobilis (MTCC 2427) A. Pranavya 1, C. Saravanamurugan 1 and S. Rajendran 2.

More information

Syamsul Falah Suryani Azmi Azhari. Department of Biochemistry Faculty of Matemathics and Natural Sciences Bogor Agricultural University

Syamsul Falah Suryani Azmi Azhari. Department of Biochemistry Faculty of Matemathics and Natural Sciences Bogor Agricultural University Bioethanol Production from Falcata (Paraserianthes falcataria) Wood by Enzymatic Delignification and Simultaneous Saccharification Fermentation using Immobilized Cells Syamsul Falah Suryani Azmi Azhari

More information

Biomass hydrolysis and ethanol production

Biomass hydrolysis and ethanol production Chapter 10 Biomass hydrolysis and ethanol production 10.1. Introduction Efficient hydrolysis of cellulosic biomass would allow its utilization for bioethanol production. Development of technologies for

More information

The Potentially Promising Technologies for Conversion Woody Biomass to Sugars for Biofuel Production: Technology and Energy Consumption Evaluation

The Potentially Promising Technologies for Conversion Woody Biomass to Sugars for Biofuel Production: Technology and Energy Consumption Evaluation The Potentially Promising Technologies for Conversion Woody Biomass to Sugars for Biofuel Production: Technology and Energy Consumption Evaluation J.Y. Zhu US Forest Service, Forest Products Laboratory,

More information

Lignin Production by Organosolv Fractionation of Lignocellulosic Biomass W.J.J. Huijgen P.J. de Wild J.H. Reith

Lignin Production by Organosolv Fractionation of Lignocellulosic Biomass W.J.J. Huijgen P.J. de Wild J.H. Reith Lignin Production by Organosolv Fractionation of Lignocellulosic Biomass W.J.J. Huijgen P.J. de Wild J.H. Reith Presented at the International Biomass Valorisation Congress, 13-15 September 2010, Amsterdam,

More information

ABENGOA BIOENERGY NEW TECHNOLOGIES

ABENGOA BIOENERGY NEW TECHNOLOGIES ABENGOA BIOENERGY NEW TECHNOLOGIES From research and demonstration to the first commercialization plant: Abengoa Bioenergy s experience in 2nd generation bioethanol November, 2012 Carmen Millan Chacartegui

More information

FEASIBILITY OF ETHANOL PRODUCTION USING THE WHOLE SUGARCANE BIOMASS

FEASIBILITY OF ETHANOL PRODUCTION USING THE WHOLE SUGARCANE BIOMASS FEASIBILITY OF ETHANOL PRODUCTION USING THE WHOLE SUGARCANE BIOMASS SANDRA CERQUEIRA PEREIRA 1, LARISSA MAEHARA 1,2, CRISTINA MARIA MONTEIRO MACHADO 3 AND CRISTIANE SANCHEZ FARINAS 1,2 1 Brazilian Agricultural

More information

to-wheels Graduate Enterprise: Bioprocessing Initiatives

to-wheels Graduate Enterprise: Bioprocessing Initiatives A Wood-to to-wheels Graduate Enterprise: Bioprocessing Initiatives David R. Shonnard Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931 Presentation to MEDC and Other

More information

By Srinivas Reddy Kamireddy Department of Chemical Engineering University of North Dakota. Advisor Dr. Yun Ji

By Srinivas Reddy Kamireddy Department of Chemical Engineering University of North Dakota. Advisor Dr. Yun Ji By Srinivas Reddy Kamireddy Department of Chemical Engineering University of North Dakota Advisor Dr. Yun Ji Outline Introduction Background Experimental procedure Results and Discussion Conclusion Acknowledgements

More information

Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature

Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature Liu et al. Biotechnology for Biofuels 2014, 7:167 RESEARCH ARTICLE Open Access Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature Zhi-Hua

More information

Simultaneous saccharification and fermentation of Arundo donax - Comparison of feeding strategies

Simultaneous saccharification and fermentation of Arundo donax - Comparison of feeding strategies Simultaneous saccharification and fermentation of Arundo donax - Comparison of feeding strategies Bhargav Prasad Kodaganti Abstract Department of Chemical Engineering, Lund University, Sweden September,

More information

Thomas Grotkjær Biomass Conversion, Business Development

Thomas Grotkjær Biomass Conversion, Business Development NOVOZYMES AND BETA RENEWABLES DEPLOY WORLD CLASS CELLULOSIC ETHANOL TECHNOLOGY TO MARKET FROM BIOMASS TO BIOENERGY BIO WORLD CONGRESS, PHILADELPHIA, 13 MAY 2014 Thomas Grotkjær Biomass Conversion, Business

More information

Sodium Hydroxide-Steam Explosion Treated Oil Palm Empty Fruit Bunch: Ethanol Production and Co- Fermentation with Cane Molasses

Sodium Hydroxide-Steam Explosion Treated Oil Palm Empty Fruit Bunch: Ethanol Production and Co- Fermentation with Cane Molasses Sodium Hydroxide-Steam Explosion Treated Oil Palm Empty Fruit Bunch: Ethanol Production and Co- Fermentation with Cane Molasses Trinset Weeraphan, a Vasana Tolieng, b Vichien Kitpreechavanich, c Somboon

More information

ENZYMATIC HYDROLYSIS OF AGRICULTURAL LIGNOCELLULOSIC BIOMASS HIDROLIZA ENZIMATICA A BIOMASEI LIGNOCELULOZICE DIN AGRICULTURA

ENZYMATIC HYDROLYSIS OF AGRICULTURAL LIGNOCELLULOSIC BIOMASS HIDROLIZA ENZIMATICA A BIOMASEI LIGNOCELULOZICE DIN AGRICULTURA Lucrări ştiinţifice Zootehnie şi Biotehnologii, vol. 42 (1) (29), Timişoara ENZYMATIC HYDROLYSIS OF AGRICULTURAL LIGNOCELLULOSIC BIOMASS HIDROLIZA ENZIMATICA A BIOMASEI LIGNOCELULOZICE DIN AGRICULTURA

More information

Requirements for characterization of biorefinery residues

Requirements for characterization of biorefinery residues COST FP0901 meeting Current needs in biorefinery analytics WG 3: Process Residues Requirements for characterization of biorefinery residues Ina Körner 1, Ron Janzon 2, Helmut Adwiraah 1, Jörn Heerenklage

More information

BIOFUELS: EUROPEAN EUROPEAN -- SPANISH OVERVIEW Mercedes Ballesteros Head of Biofuels Unit CIEMAT 3rd March,

BIOFUELS: EUROPEAN EUROPEAN -- SPANISH OVERVIEW Mercedes Ballesteros Head of Biofuels Unit CIEMAT 3rd March, BIOFUELS: EUROPEAN - SPANISH OVERVIEW Mercedes Ballesteros Head of Biofuels Unit CIEMAT 3rd March, 2011 1 OUTLINE CURRENT BIOFUELS PRODUCTION NEW OBJECTIVES FOR 2020 SECOND GENERATION BIOFUELS CIEMAT s

More information

Cellulosic Conversion to Bioethanol from Pongamia Pod A Biodiesel Industry Waste

Cellulosic Conversion to Bioethanol from Pongamia Pod A Biodiesel Industry Waste International OPEN ACCESS Journal Of Modern Engineering Research (IJMER) Cellulosic Conversion to Bioethanol from Pongamia Pod A Biodiesel Industry Waste Yashaswi R.Metri 1, Dr.Bharati S.Meti 2 Department

More information

Saccharification versus simultaneous saccharification and fermentation of kraft pulp

Saccharification versus simultaneous saccharification and fermentation of kraft pulp 48 March, 2012 Int J Agric & Biol Eng Vol. 5 No.1 Saccharification versus simultaneous saccharification and fermentation of kraft pulp Nichole A. Bauer, William R. Gibbons (Biology-Microbiology Department,

More information

Challenges of Ethanol Production from Lignocellulosic Biomass

Challenges of Ethanol Production from Lignocellulosic Biomass Challenges of Ethanol Production from Lignocellulosic Biomass Maha Dakar Varieties of Carbohydrates Sugar Starch Cellulose/Hemicellulose What All Plants Have in Common Cellulose Glucose Why is it difficult

More information

HIDROLIZA ENZIMATICA CU PRETRATAMENT A PLANTELOR ENERGETICE AGRICOLE IN SCOPUL OBTINERII DE BIOCOMBUSTIBILI (ETANOL, BIOGAZ)

HIDROLIZA ENZIMATICA CU PRETRATAMENT A PLANTELOR ENERGETICE AGRICOLE IN SCOPUL OBTINERII DE BIOCOMBUSTIBILI (ETANOL, BIOGAZ) Lucrări ştiinţifice Zootehnie şi Biotehnologii, vol. 42 (1) (2009), Timişoara HYDROLYSIS OF AGRICULTURAL BIOMASS BY COMBINED PRETREATMENT AND ENZYMATIC METHODS IN ORDER TO PRODUCE BIOFUELS (ETHANOL, BIOGAS)

More information

IBUS Integrated Biomass Utilisation Systems

IBUS Integrated Biomass Utilisation Systems IBUS Integrated Biomass Utilisation Systems Best Basis for Biorefineries Børge Holm Christensen Holm Christensen Biosystemer ApS, Denmark Charles Nielsen Dong Energy A/S Denmark IEA Workshop TheBiorefinery

More information

INDUSTRIAL ENZYMES FOR CELLULOSIC SUGARS AND BEYOND

INDUSTRIAL ENZYMES FOR CELLULOSIC SUGARS AND BEYOND INDUSTRIAL ENZYMES FOR CELLULOSIC SUGARS AND BEYOND THE POWER AND SPEED OF TAILORED SOLUTIONS EuroNanoForum 2017 Malta, June 22 nd MATTI HEIKKILÄ CTO METGEN STRATEGIC FOCUS Lignocellulosic biomass conversion

More information

Enzymatic hydrolysis of steam-exploded sugarcane bagasse by adding natural Sapindus peel

Enzymatic hydrolysis of steam-exploded sugarcane bagasse by adding natural Sapindus peel 5th International Conference on Advanced Design and Manufacturing Engineering (ICADME 215) Enzymatic hydrolysis of steam-exploded sugarcane bagasse by adding natural Sapindus peel 1 Y.Z. YOU & 2 Y.M. WANG

More information

DBT-IOC Integrated Technology for 2G Ethanol

DBT-IOC Integrated Technology for 2G Ethanol DBT-IOC Integrated Technology for 2G Ethanol Dr S K Puri Chief General Manager (Bio-Energy) Indian Oil Corporation Limited R & D Centre, Sector-13 Faridabad -121007 (Haryana) EU-India Conference on Advanced

More information

The possibility of longan tree trimming waste for the bioethanol production

The possibility of longan tree trimming waste for the bioethanol production Available online at www.buuconference.buu.ac.th The 5 th Burapha University International Conference 2016 Harmonization of Knowledge towards the Betterment of Society The possibility of longan tree trimming

More information

RESEARCH ARTICLE. Keywords: bioethanol, lignocellulosic biomass, sugarcane bagasse derived cellulignin.

RESEARCH ARTICLE. Keywords: bioethanol, lignocellulosic biomass, sugarcane bagasse derived cellulignin. Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.13 No.2, Issue of March 15, 2010 2010 by Pontificia Universidad Católica de Valparaíso -- Chile Received May 11, 2009 / Accepted October 14, 2009

More information

Ethanol production potential from AFEX and steam exploded sugarcane residues for sugarcane biorefineries

Ethanol production potential from AFEX and steam exploded sugarcane residues for sugarcane biorefineries Mokomele et al. Biotechnol Biofuels (21) 11:127 https://doi.org/1.116/s136-1-113-z Biotechnology for Biofuels RESEARCH Open Access Ethanol production potential from AFEX and steam exploded sugarcane residues

More information

Rice Straws and Husks Biofuel: Emphasizing on Selection of Pre-Treatment Method Elza Firdiani Shofia, Kharisma Bangsa Senior High School, Indonesia

Rice Straws and Husks Biofuel: Emphasizing on Selection of Pre-Treatment Method Elza Firdiani Shofia, Kharisma Bangsa Senior High School, Indonesia Rice Straws and Husks Biofuel: Emphasizing on Selection of Pre-Treatment Method Elza Firdiani Shofia, Kharisma Bangsa Senior High School, Indonesia Picture: Indonesian farmers are harvesting rice. There

More information

Trash into Gas: Powering Sustainable Transportation by Plants

Trash into Gas: Powering Sustainable Transportation by Plants Trash into Gas: Powering Sustainable Transportation by Plants Jaclyn D. DeMartini Dr. Charles E. Wyman University of California Chemical and Environmental Engineering Department Center for Environmental

More information

Available online at ScienceDirect. Procedia Engineering 90 (2014 )

Available online at  ScienceDirect. Procedia Engineering 90 (2014 ) Available online at www.sciencedirect.com ScienceDirect Procedia Engineering 90 (2014 ) 504 509 10th International Conference on Mechanical Engineering, ICME 2013 Production of bio-fuel (bio-ethanol) from

More information

Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol

Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol Industrial Crops and Products 20 (2004) 103 110 Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol Zs. Kádár, Zs. Szengyel, K. Réczey Department of

More information

Production Of Fermentable Sugars By Dilute Acid Pretreatment And Enzymatic Saccharification Of Three Different Lignocellulosic Materials

Production Of Fermentable Sugars By Dilute Acid Pretreatment And Enzymatic Saccharification Of Three Different Lignocellulosic Materials International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.4, No.4, pp 1497-1502, Oct-Dec 2012 Production Of Fermentable Sugars By Dilute Acid Pretreatment And Enzymatic Saccharification

More information

Bioethanol production: from wood to fuel

Bioethanol production: from wood to fuel Bioethanol production: from wood to fuel JM van Zyl α, LR Lynd β and TM Harms α. α Department of Mechanical and Mechatronic Engineering, Stellenbosch University, RSA β Thayer School of Engineering, Dartmouth

More information

Technical Barriers in Converting Lignocellulose to ethanol. Samson Hailemichael Introduction to Green Chemistry (CHEM 0671) Dec.

Technical Barriers in Converting Lignocellulose to ethanol. Samson Hailemichael Introduction to Green Chemistry (CHEM 0671) Dec. Technical Barriers in Converting Lignocellulose to ethanol Samson Hailemichael Introduction to Green Chemistry (CHEM 0671) Dec. 15, 2009 Outline Introduction Benefits Drawbacks Conventional process Pretreatment

More information

Introduction to BIOFUELS. David M. Mousdale. CRC Press. Taylor & Francis Group Boca Raton London New York

Introduction to BIOFUELS. David M. Mousdale. CRC Press. Taylor & Francis Group Boca Raton London New York Introduction to BIOFUELS David M. Mousdale CRC Press Taylor & Francis Group Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Croup, an informa business Contents Preface Acknowledgments

More information

Available online at Journal of Agroalimentary Processes and Technologies 2013, 19(4),

Available online at   Journal of Agroalimentary Processes and Technologies 2013, 19(4), Available online at http://journal-of-agroalimentary.ro Journal of Agroalimentary Processes and Technologies 2013, 19(4), 419-428 Journal of Agroalimentary Processes and Technologies Cost effective production

More information

Rubbish or resources: an investigation into converting municipal solid waste to bio-ethanol production

Rubbish or resources: an investigation into converting municipal solid waste to bio-ethanol production Waste to Energy 29 Rubbish or resources: an investigation into converting municipal solid waste to bio-ethanol production A. Li & M. Khraisheh University College London, UK Abstract An investigation into

More information

Cellulosic ethanol from agricultural residues THINK AHEAD, THINK SUNLIQUID

Cellulosic ethanol from agricultural residues THINK AHEAD, THINK SUNLIQUID Cellulosic ethanol from agricultural residues THINK AHEAD, THINK SUNLIQUID Market ready and highly efficient SUNLIQUID PROCESS FOR THE PRODUCTION OF CELLULOSIC ETHANOL By developing sunliquid technology,

More information

The Utility of Critical Fluids for Efficient Processing of Lignocellulose as Part of an Integrated Biorefining Concept

The Utility of Critical Fluids for Efficient Processing of Lignocellulose as Part of an Integrated Biorefining Concept University of Birmingham, School of Chemical Engineering, College of Engineering & Physical Sciences, UK The Utility of Critical Fluids for Efficient Processing of Lignocellulose as Part of an Integrated

More information

SIMULTANEOUS SACCHARIFICATION and FERMENTATION of BANANA (MUSA ACUMINATA) PEEL for BIOETHANOL PRODUCTION An T.T. Tran 1, Quan D.

SIMULTANEOUS SACCHARIFICATION and FERMENTATION of BANANA (MUSA ACUMINATA) PEEL for BIOETHANOL PRODUCTION An T.T. Tran 1, Quan D. SIMULTANEOUS SACCHARIFICATION and FERMENTATION of BANANA (MUSA ACUMINATA) PEEL for BIOETHANOL PRODUCTION An T.T. Tran 1, Quan D. Nguyen 1,2, Phung T.K. Le 2 1 1 Laboratory of Biofuel and Biomass Research

More information

Wood to Wheel: Process Improvement for the Production of Substituted Fuels from Renewable Biomass

Wood to Wheel: Process Improvement for the Production of Substituted Fuels from Renewable Biomass Wood to Wheel: Process Improvement for the Production of Substituted Fuels from Renewable Biomass POKE Symposium 10-16.08.2014, Ösel Venkata Prabhakar Soudham venkata.soudham@chem.umu.se The Problem: Our

More information

Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus

Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces marxianus Energies 2013, 6, 1794-1801; doi:10.3390/en6031794 Article OPEN ACCESS energies ISSN 1996-1073 www.mdpi.com/journal/energies Production of Bioethanol from Carrot Pomace Using the Thermotolerant Yeast Kluyveromyces

More information

Fed-Batch Mode Optimization of SSF for Cellulosic Ethanol Production from Steam-Exploded Corn Stover

Fed-Batch Mode Optimization of SSF for Cellulosic Ethanol Production from Steam-Exploded Corn Stover Fed-Batch Mode Optimization of SSF for Cellulosic Ethanol Production from Steam-Exploded Corn Stover Zhen Wang, Zhe Lv, Xiushan Yang, and Shen Tian* To achieve a desired ethanol concentration and maximize

More information

Prof. Arkady P.Sinitsyn

Prof. Arkady P.Sinitsyn Enzyme complex for highly efficient saccharification of lignocellulosic feedstocks Prof. Arkady P.Sinitsyn A.N.Bach Institute of Biochemistry, Russian Academy of Sciences German-Russian Forum Biotechnology

More information

Activities in UW Forest Resources and Lignocellulosic Biorefineries

Activities in UW Forest Resources and Lignocellulosic Biorefineries Activities in UW Forest Resources and Lignocellulosic Biorefineries Rick Gustafson, Renata Bura, Bill McKean, Sharon Doty, Brian Marquardt, Rob Synovec, Joyce Cooper 3 May 2010 U.S. Renewable Fuel Standard

More information

Evaluation of Enzymatic Hydrolysis Process of Sugarcane Bagasse

Evaluation of Enzymatic Hydrolysis Process of Sugarcane Bagasse Evaluation of Enzymatic Hydrolysis Process of Sugarcane Bagasse FAPESP-OXITENO LABORATORY OF INDUSTRIAL BIOTECHNOLOGY CENTER FOR TECHNOLOGY OF PROCESSES AND PRODUCTS The Institute for Technological Research

More information

Hydrothermal treatments of corn cob and hemicelluloses extraction

Hydrothermal treatments of corn cob and hemicelluloses extraction Proceedings of the 10th International Chemical and Biological Engineering Conference - CHEMPOR 2008 Braga, Portugal, September 4-6, 2008 E.C. Ferreira and M. Mota (Eds.) Hydrothermal treatments of corn

More information

Sugar Industry Restructuring by Implementing Biorefinery Technology

Sugar Industry Restructuring by Implementing Biorefinery Technology Sugar Industry Restructuring by Implementing Biorefinery Technology Dr. Maurizio Cocchi THE BIOREFINERY CONCEPT Biorefinery approach Integration of biomass conversion processes and technologies to produce

More information

Does densification (pelletisation) restrict the biochemical conversion of biomass?

Does densification (pelletisation) restrict the biochemical conversion of biomass? Does densification (pelletisation) restrict the biochemical conversion of biomass? Linoj Kumar, Zahra Tooyserkani Shahab Sokhansanj, Richard Chandra Jack Saddler* Forest Products Biotechnology/Bioenergy

More information

Research in Biotechnology, 2(4): 01-06, 2011 ` ISSN: X

Research in Biotechnology, 2(4): 01-06, 2011 ` ISSN: X Research in Biotechnology, 2(4): 01-06, 2011 ` ISSN: 2229-791X www.researchinbiotechnology.com Regular Article Depolymerization of bagasse by Ruminococcus albus in the production of eco-friendly fuel Sampana

More information

Extraction of high molecular mass hemicelluloses prior to ethanol production. Alkali steam pretreatment of wheat and barley straw. Elisabeth Joelsson

Extraction of high molecular mass hemicelluloses prior to ethanol production. Alkali steam pretreatment of wheat and barley straw. Elisabeth Joelsson Extraction of high molecular mass hemicelluloses prior to ethanol production Alkali steam pretreatment of wheat and barley straw Elisabeth Joelsson Department of Chemical Engineering Lund University P.O.

More information

Moving towards commercialization of lignocellulosic biomass to fuels to chemicals. How to deal with heterogeneous biomass?

Moving towards commercialization of lignocellulosic biomass to fuels to chemicals. How to deal with heterogeneous biomass? Moving towards commercialization of lignocellulosic biomass to fuels to chemicals. How to deal with heterogeneous biomass? Renata Bura, Shannon Ewanick, Erik Budsberg, Jordan Crawford, Brian Marquardt

More information

Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production and characterization of cellulases

Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production and characterization of cellulases Journal of Scientific & Industrial Research 714 Vol. 68, August 29, pp. 714-718 J SCI IND RES VOL 68 AUGUST 29 Solid-state fermentation of waste cabbage by Penicillium notatum NCIM NO-923 for production

More information

HOW LIME PRETREATMENT IMPROVES YOUR BIOGAS YIELD

HOW LIME PRETREATMENT IMPROVES YOUR BIOGAS YIELD HOW LIME PRETREATMENT IMPROVES YOUR BIOGAS YIELD Heiszwolf, J.J. 1, Dobbe, R. 2 and Brognaux, N. 1 1 Lhoist Business Innovation Center, Rue de l Industrie 31, B-1400, Belgium, 2 Lhoist Western Europe,

More information

BIOETHANOL PRODUCTION FROM RICE BRAN BY SACCHAROMYCES CEREVISIAE. *Corresponding author:

BIOETHANOL PRODUCTION FROM RICE BRAN BY SACCHAROMYCES CEREVISIAE. *Corresponding author: BIOETHANOL PRODUCTION FROM RICE BRAN BY SACCHAROMYCES CEREVISIAE K. Harismah 1, M. Da i 2, A. Asngad 3, Samlawi 4 1 Department of Chemical Engineering, 2 Department of Biology Education, 3 Faculty of Pharmacy

More information

Update on Lignol s Biorefinery Technology

Update on Lignol s Biorefinery Technology Update on Lignol s Biorefinery Technology October 15, 2009 www.lignol.ca Introduction to Lignol A leader in emerging cellulosic ethanol technology Burnaby, BC and Berwyn, PA based, public company (LEC:TSX-V)

More information

Comparison of Acidic and Basic Pretreatment on Saccharification of Water Hyacinth

Comparison of Acidic and Basic Pretreatment on Saccharification of Water Hyacinth Comparison of Acidic and Basic Pretreatment on Saccharification of Water Hyacinth Sathvik Varma V 1, Hari Narayanan T R 2, Kamala K 3, Shivashankar B 4, Jagadish H Patil 5 Corresponding author, UG Chemical

More information

Production of cellulase by submerged and solid-state cultures and yeasts selection for conversion of lignocellulose to ethanol

Production of cellulase by submerged and solid-state cultures and yeasts selection for conversion of lignocellulose to ethanol Romanian Biotechnological Letters Vol. 14, No. 2, 2009, pp. 4275-4281 Copyright 2009 Bucharest University Printed in Romania. All rights reserved Romanian Society of Biological Sciences ORIGINAL PAPER

More information

Alternative Feed-stocks for Bioconversion to Ethanol: a techno-commercial appraisal

Alternative Feed-stocks for Bioconversion to Ethanol: a techno-commercial appraisal Alternative Feed-stocks for Bioconversion to Ethanol: a techno-commercial appraisal Subhash Chand Formerly, Professor & Head: Department of Biochemical Engineering & Biotechnology Indian Institute of Technology

More information

Evaluation of agricultural wastes for the use in ethanol production by Candida shehatae TISTR 5843

Evaluation of agricultural wastes for the use in ethanol production by Candida shehatae TISTR 5843 KKU Res. J. 2014; 19(Supplement Issue) 11 KKU Res. J. 2014; 12(Supplement Issue): 11-18 http : //resjournal.kku.ac.th Evaluation of agricultural wastes for the use in ethanol production by Candida shehatae

More information

CBSE-2014 [2 nd and 3 rd April 2014] Challenges in Biochemical Engineering and Biotechnology for Sustainable Environment

CBSE-2014 [2 nd and 3 rd April 2014] Challenges in Biochemical Engineering and Biotechnology for Sustainable Environment International Journal of ChemTech Research CODEN (USA): IJCRGG ISSN : 0974-4290 Vol.6, No.12, pp 5064-5070, October 2014 CBSE-2014 [2 nd and 3 rd April 2014] Challenges in Biochemical Engineering and Biotechnology

More information

Optimization of Enzymatic Hydrolysis Process of Mischantus giganteus Plants, which were Physical/Chemical Pretreated to Obtain Biogas

Optimization of Enzymatic Hydrolysis Process of Mischantus giganteus Plants, which were Physical/Chemical Pretreated to Obtain Biogas Bulletin UASVM Animal Science and Biotechnologies, 68(1-2)/2011 Print ISSN 1843-5262; Electronic ISSN 1843-536X Optimization of Enzymatic Hydrolysis Process of Mischantus giganteus Plants, which were Physical/Chemical

More information

Effect of Liquid Hot Water Pretreatment on Switchgrass Hydrolysis

Effect of Liquid Hot Water Pretreatment on Switchgrass Hydrolysis Effect of Liquid Hot Water Pretreatment on Switchgrass Hydrolysis Youngmi Kim, Nathan S. Mosier, Michael R. Ladisch Laboratory of Renewable Resources Engineering (LORRE) Purdue University Presented at

More information

Optimization of microwave assisted alkali pretreatment and enzymatic hydrolysis of Banana pseudostem for bioethanol production

Optimization of microwave assisted alkali pretreatment and enzymatic hydrolysis of Banana pseudostem for bioethanol production 2011 2nd International Conference on Environmental Science and Technology IPCBEE vol.6 (2011) (2011) IACSIT Press, Singapore Optimization of microwave assisted alkali pretreatment and enzymatic hydrolysis

More information