Effects of Sewage Run off on River Water Quality. John Peilert Central Catholic High school grade 9 2nd year of PJAS
|
|
- Rosanna Wiggins
- 6 years ago
- Views:
Transcription
1 Effects of Sewage Run off on River Water Quality John Peilert Central Catholic High school grade 9 2nd year of PJAS
2 Problem The sewage system in Pittsburgh is one of the oldest in the country. Whenever it rains the water treatment plant diverts the sewage directly into the river. As a city that relies heavily on its rivers, this could be devastating.
3 Storm Water Runoff Runoff is part of the water cycle. It is water that flows over a land surface. Precipitation causes substances to enter bodies of water unchecked.
4 Sewer Runoff Combined Sewer System collects both waste and rain water. This allows for both storm water and waste water to enter the sanitation plant. Overflow causes waste water to enter water bodies. Pittsburgh s municipalities mainly have combined
5 Nitrate Nitrates are used as components in fertilizers. Nitrates can also be used as a salt.
6 Ammonia Ammonia is used in the home as a cleaner. Also a component in fertilizer.
7 Phosphate Used is an ingredient in soap It is also used as a component of fertilizer.
8 E. coli Gram negative Rod-shaped Intestinal mammalian symbiont Widely studied Easy to maintain, high growth rate, simple nutritional requirements
9 Enterobactor aerogenes Gram negative Rod shaped Found in human intestinal tract Is an opportunistic infector
10 Purpose The purpose of this experiment is to determine whether or not sewage will significantly affect the levels of chemicals and fecal matter in the river water.
11 Hypothesis Null: The river water affected by the sewer runoff will not vary significantly from the normal river water. Alternative: The Coliscans that test the water collected after the precipitation will contain significantly more fecal and non-fecal coliforms.
12 Materials Carolina spectrophotometer nitrate solution 10mg/1L(stock) phosphate solution 10mg/1L(stock) ammonia solution 10mg/1L(stock) nitrate testing kit phosphate testing kit ammonia testing kit Incubator 10ml sterile test tubes 10 & 5 ml pipette 1000μL micropipette water test tube rack river water samples river water samples with sewer runoff Coliscan easy gel Coliscan easy gel plates
13 Procedure Two samples of river water were collected. The first was collected on January 4. The sample was collected then because there was almost no precipitation. The second was collected on the 12 a day after four straight days of precipitation. A 3mL sample was added to the Coliscan fluid and then immediately poured into a sterile Coliscan plate After an hour of hardening the plates were then incubated over night at 37C. The resulting colonies were identified and counted. Three standard curves were then established using the following solutions (The stock is the amount of either nitrate, phosphate, or ammonia in solution. All were used.)
14 Procedure 0% 20% 40% 60% 80% 100% Pure water 5ml 4ml 3ml 2ml 1ml 0ml Stock 0ml 1ml 2ml 3ml 4ml 5ml total 5ml 5ml 5ml 5ml 5ml 5ml After the solutions were made, a reporter molecule packet was added to each of the corresponding test tubes. The samples were allowed to sit for 10 minutes, to promote the colorimetric reaction. Absorbance readings at 400 nm were recorded for all samples.
15 absorbance Standard Curve Ammonia Series1 Poly. (Series1) % 50% 100% 150% [Concentration] mg/l
16 Absorbance Standard Curve 0.2 Phosphate Series1 Poly. (Series1) % 50% 100% 150% [Concentration] mg/l
17 absorbance Standard Curve 0.9 Nitrate Series1 Poly. (Series1) % 50% 100% 150% [concentration] mg/l
18 mg/l Runoff chemical results Phosphate nitrate ammonia River water without sewage River water with sewage
19 Results Standard Curve 0% 20% 40% 60% 80% 100% Phosphate average Nitrate average Ammonia average Absorbance of river water samples River water without sewage mg/l River water with sewage mg/l Phosphate average Nitrate average Ammonia average.5.525
20 Phosphate Anova Anova: Single Factor Phosphates SUMMARY Groups Count Sum Average Variance River water without runoff River water with runoff ANOVA Source of Variation SS df MS F P-value F crit Between Groups Within Groups Total
21 Ammonia Anova Anova: Single Factor Ammonia SUMMARY Groups Count Sum Average Variance River water without runoff River water with runoff ANOVA Source of Variation SS df MS F P-value F crit Between Groups Within Groups Total
22 Nitrate Anova Anova: Single Factor Nitrate SUMMARY Groups Count Sum Average Variance River water without runoff River water with runoff ANOVA Source of Variation SS df MS F P-value F crit Between Groups Within Groups Total
23 Coliscan Results Three types of colonies were identified. E. coli (fecal) Enterobacter Aerogenes (non-fecal) Unidentified coliforms (other) Average number of coliforms E. coli Enterobacter aerogenes Other River water without sewage River Water with sewage
24 Number of colonies Coliscan Results River water with sewage Fecal coliforms non fecal coliforms other river water without sewage sewage
25 E. Coli Anova Anova: Single Factor E. coli SUMMARY Groups Count Sum Average Variance River water without runoff River water with runoff ANOVA Source of Variation SS df MS F P-value F crit Between Groups Within Groups Total 4 7
26 Enterobactor aerogenes Anova Anova: Single Factor Enterobacter aerogenes SUMMARY Groups Count Sum Average Variance River water without runoff River water with runoff ANOVA Source of Variation SS df MS F P-value F crit Between Groups Within Groups Total
27 Unidentified coliforms Anova Anova: Single Factor Other SUMMARY Groups Count Sum Average Variance River water without runoff River water with runoff ANOVA Source of Variation SS df MS F P-value F crit Between Groups Within Groups Total
28 Conclusion Nitrate was significant. Phosphate and Ammonia were not significant The count of non-fecal coliform colonies changed significantly. The count of unidentified coliforms and E. coli coliforms were not changed significantly. The majority of tests did not change significantly.
29 Limitations and extensions Only one wavelength of light was used during the tests. The samples for the standard curve should have sterile filtered. There should have been more replicates to receive more accurate results on the Coliscan. The test tubes were not polished before having the absorbance measured. More samples should have been taken when more or less rain had fallen. Algae growth should be tested with each sample. Multiple rivers should be tested in multiple places.
Andrew Klabnik Grade 9 Central Catholic High School
Andrew Klabnik Grade 9 Central Catholic High School Part of the water cycle Water that flows over a land surface Precipitation causes substances to enter bodies of water unchecked Petroleum Pesticides
More informationEffects of Splenda on E.coli Survivorship. Noah Stickel Central Catholic High School Grade 9 First Year of PJAS
Effects of Splenda on E.coli Survivorship Noah Stickel Central Catholic High School Grade 9 First Year of PJAS Microbial Flora Normal populations of eukaryotic fungi, protists, and bacteria, varies between
More informationBy: Nicholas Bhaskar Pittsburgh Central Catholic Grade 9
Oil Pollution Effects on Autotroph Population By: Nicholas Bhaskar Pittsburgh Central Catholic Grade 9 Crude Oil Found beneath Earth s surface Made from dead organisms under sedimentary rock that undergo
More informationFRACKING CHEMICAL. Luke Deasy Central Catholic High School Grade 9 1st Year in PJAS. Effect on Microbial Life. Tuesday, January 29, 13
FRACKING CHEMICAL Effect on Microbial Life Luke Deasy Central Catholic High School Grade 9 1st Year in PJAS PROBLEM World s supply of natural gas is depleting A number of people believe that we must find
More informationThe Effects of Gamma Radiation on Microbial Life. Nolan Jacob 9 th Grade Central Catholic High School
The Effects of Gamma Radiation on Microbial Life Nolan Jacob 9 th Grade Central Catholic High School Gamma Radiation Produce extremely high frequency radiation Used in the food industry to control the
More informationEffect of Bacterial Growth Stage on Chlorine Disinfection. Project By: Warren Sipe Grade 9 Central Catholic High School
Effect of Bacterial Growth Stage on Chlorine Disinfection Project By: Warren Sipe Grade 9 Central Catholic High School Chlorine Bleach -Common household disinfectant -Active ingredients -Sodium hypochlorite
More informationTAML Remediation of Eutrophic Water SIMON SWEENEY CENTRAL CATHOLIC HIGH SCHOOL 9TH GRADE
TAML Remediation of Eutrophic Water SIMON SWEENEY CENTRAL CATHOLIC HIGH SCHOOL 9TH GRADE Problem u Water pollution harms the ecosystem, a solution must be found to combat the pollution by degrading the
More informationArtificial Sweeteners Mutagenic Effects. Warren Austin Pittsburgh Central Catholic High School Second Year at PJAS
Artificial Sweeteners Mutagenic Effects Warren Austin Pittsburgh Central Catholic High School Second Year at PJAS Artificial Sweeteners Maybe derived from naturally occurring substances Excessive sweetness
More informationEffects of Raspberry Juice on Microbial Flora. Max O Malley Grade 9 Central Catholic High School
Effects of Raspberry Juice on Microbial Flora Max O Malley Grade 9 Central Catholic High School Antimicrobial effects of berries Types of berries Raspberry Cranberry Blueberry Previous Studies Cancer Cells
More informationLab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl)
Lab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl) Some of the diseases that humans can contract from drinking polluted water include typhoid, dysentery,
More informationMeasuring Dissolved Oxyg Instructions for the La Motte Field-Test Kit
Then, immediately add 8 drops of alkaline potassium iodide azide to the water sample Recap the bottle and shake to mix. Immediately add 8 drops of manganous sulfate to the water Record the temperature
More informationLead Compound Effects on Microbial Survivorship. Joseph Ga; Central Catholic High School 9 th grade
Lead Compound Effects on Microbial Survivorship Joseph Ga; Central Catholic High School 9 th grade Microbial Flora Li@le is known about the associacon between humans and their flora. Effects are mutualiscc,
More informationThe Effects of Isotretinoin on C2C12 Stem Cells. Colm Parrish Pi,sburgh Central Catholic HS Grade 11
The Effects of Isotretinoin on C2C12 Stem Cells Colm Parrish Pi,sburgh Central Catholic HS Grade 11 Tissue Engineering The development and manipulation of artificial implants, laboratory-grown tissues,
More informationPROTECTING THE WACCAMAW WATERSHED. Water Quality Monitoring. Christine Ellis. Waccamaw RIVERKEEPER A Program of Winyah Rivers Foundation
PROTECTING THE WACCAMAW WATERSHED Water Quality Monitoring Christine Ellis Waccamaw RIVERKEEPER A Program of Winyah Rivers Foundation Focusing on the Waccamaw Waccamaw RIVERKEEPER Program Citizen-Based
More informationAdapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692
Biology 4B Laboratory Bacteriological Examination of Water Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692 Objectives Carry out
More informationLab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab
Lab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab OBJECTIVES 1. Understand the use of MPN to determine likely fecal water contamination. 2. Understand the use of MUG,
More information2014 ASSINIBOINE RIVER MONITORING REPORT
October 15, 2014 Temperature º C 9.7 9.6 9.6 9.6 9.7 9.7 10.1 10.2 10.1 Dissolved Oxygen mg/l 10.3 10.2 10.2 10.3 10.1 10.2 10.2 10.2 10.2 Conductivity ms/cm 1.262 1.235 1.253 1.307 1.315 1.307 1.322 1.32
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More informationWater Chemistry Monitoring
Chicago River Fieldtrip Activity Summary Students use basic to advanced water quality monitoring kits and probes to assess the water quality of the Chicago River. Water chemistry kits available for loan
More informationGrand River Monitoring
City of Grand Rapids Environmental Services Grand River Monitoring by Mike Lunn Environmental Services Department Manager Grand River Monitoring History 1968 Grand River Monitoring Network Since 1988 Data
More information2009 Water Sampling Report
2009 Water Project No. 209063-000 January 2010 , Idaho 2009 Water 10394 02/11/10 Project No. 209063-000 January 2010 TABLE OF CONTENTS 10-1.0 Introduction 1.1 Background 1 1.2 Objectives 1 2.0 - Island
More informationAcidity and Alkalinity:
Evaluation of Pollution Sources to Lake Glenville Quarterly Report December 2018 Kimberlee K Hall, PhD Environmental Health Program, Western Carolina University Summary Chemical and microbial analysis
More informationTAML Process Effluent Effect on Aquatic Productivity. Joe DiPietro Central Catholic High School 11 th grade
TAML Process Effluent Effect on Aquatic Productivity Joe DiPietro Central Catholic High School 11 th grade Tetra Amido Macrocyclic Ligand(TAML) Developed and engineered by a research group led by Dr. Terry
More informationThe Effects of EGCG on Microbiological Survivorship. Sean Bailey Grade 11, CCHS 2nd Year in PJAS
The Effects of EGCG on Microbiological Survivorship Sean Bailey Grade 11, CCHS 2nd Year in PJAS This Experiment to determine whether or not epigallocatechin-3-gallate has a anti-carcinogenic effect on
More informationThird Herring Brook Watershed
Bridgewater State University Virtual Commons - Bridgewater State University Watershed Access Lab Projects Watershed Access Lab 2004 Third Herring Brook Watershed Recommended Citation Norwell High School,
More informationSwab and Samplers Test Kits Quality control as easy as 1, 2, 3
Swab and Samplers Test Kits Quality control as easy as 1, 2, 3 Merck Millipore is a division of Environmental monitoring Sample, incubate, count Merck Millipore s Samplers and Swab Test Kits simplify routine
More informationBefore You Begin. Calibration Protocols
Before You Begin Read through this entire protocol sheet carefully before you start your experiment and prepare any materials you may need. Calibration Protocols 1. OD 600 Reference point You will use
More informationigem 2018 InterLab Study Protocol Before You Begin
igem 2018 InterLab Study Protocol Before You Begin Read through this entire protocol sheet carefully before you start your experiment and prepare any materials you may need. In order to improve reproducibility,
More informationNational Science Content Standards: A: Science as Inquiry E: Science and Technology
National Science Content Standards: A: Science as Inquiry E: Science and Technology To develop an understanding of water quality monitoring and an appreciation of the methods used to keep water safe and
More informationFactsheet: Town of Deep River Water Quality and Stormwater Summary
79 Elm Street Hartford, CT 06106-5127 www.ct.gov/deep Affirmative Action/Equal Opportunity Employer Factsheet: Town of Deep River Water Quality and Stormwater Summary This document was created for each
More informationSummary. River. 5 th 8 th. about the. Chemical. anteceden. Grade Level: 5 th. 12 th. Objectives: instructions to. determine the. Chicago River Field
Water Chemistry Monitoring Chicago River Field Trip Activity Summary Students use basic to advanced water quality monitoring kits and probes to assess the water quality of the Chicago River. Water chemistry
More informationValidation of qpcr Rapid Bacterial Quantification through Viable E. coli cell count in the Saginaw Bay Watershed
Validation of qpcr Rapid Bacterial Quantification through Viable E. coli cell count in the Saginaw Bay Watershed TYLER LEFEVRE ADVISOR: DR. TAMI SIVY 1 Overview Introduction to fecal coliforms Current
More informationHe eia Fishpond Water Monitoring Project Due:
He eia Fishpond Water Monitoring Project Due: While on your field trip to He eia Fishpond, you will be using a water monitoring kit. This kit was designed to provide a simple, affordable, non-hazardous
More informationFactsheet: City of West Haven Water Quality and Stormwater Summary
79 Elm Street Hartford, CT 06106-5127 www.ct.gov/deep Affirmative Action/Equal Opportunity Employer Factsheet: City of West Haven Water Quality and Stormwater Summary This document was created for each
More informationFactsheet: Town of East Lyme Water Quality and Stormwater Summary
79 Elm Street Hartford, CT 06106-5127 www.ct.gov/deep Affirmative Action/Equal Opportunity Employer Factsheet: Town of East Lyme Water Quality and Stormwater Summary This document was created for each
More informationE.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps
E.Z.N.A. HP Viral RNA/DNA Kit R6873-00 5 preps R6873-01 50 preps August 2013 E.Z.N.A. HP Viral RNA/DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationJoe DiPietro Central Catholic High School 11 th grade
Joe DiPietro Central Catholic High School 11 th grade Developed and engineered by a research group led by Dr. Terry Collins of Carnegie Mellon University Accelerates Hydrogen Peroxide(H2O2) reaction Made
More informationITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector
Page 1 of 5 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector 1. Digest 1 µg of pbluescript with Eco RI 2. Following digestion, add 0.1 volumes of 3M sodium acetate (ph
More informationWrite your own lab Spectrophotometry/Colorimetry
Sheila MacIntyre University of Pennsylvania Spectroscopy Nitrate Concentration Determination Teacher Section: Background Information: In this lab students will determine the concentration of nitrates in
More informationIntroduction to Bacteria Monitoring of Surface water for Volunteers. Is the water safe?
Slide 1 Introduction to Bacteria Monitoring of Surface water for Volunteers CWQ Winter Session February 7, 2004 Slide 2 Is the water safe? In part, the answer to this? depends on presence or absence of
More informationWater Quality Tests Explained
Chicago River Classroom Activity Summary When doing water quality testing it is important that students understand how each of the eight parameters affects the river. This demonstration can help teachers
More informationFactsheet: Town of Trumbull Water Quality and Stormwater Summary
79 Elm Street Hartford, CT 06106-5127 www.ct.gov/deep Affirmative Action/Equal Opportunity Employer Factsheet: Town of Trumbull Water Quality and Stormwater Summary This document was created for each town
More informationFactsheet: Town of Hamden Water Quality and Stormwater Summary
79 Elm Street Hartford, CT 06106-5127 www.ct.gov/deep Affirmative Action/Equal Opportunity Employer Factsheet: Town of Hamden Water Quality and Stormwater Summary This document was created for each town
More informationPLANS for the Chesapeake Bay A Teacher s Guide
1 Nutrient Enrichment of Phytoplankton in the Chesapeake Estuary A MWEE for 9 th Grade Environmental Science Classes Third Day of PLANS Teacher led classroom activity, supported by PLANS staff through
More informationSome Industrially Important Microbes and Their Products
2 Some Industrially Important Microbes and Their Products 2.1. ENZYME PRODUCING MICROBES Type of enzyme Substrate Microorganism Amylase Starch Saccharomyces diastaticus Protease Proteins Bacillus sp. Lipase
More informationWater Quality Assessment for the Town of Caswell Beach, 2008
Water Quality Assessment for the Town of Caswell Beach, 2008 UNCW-CMS Report 08-02 Report to: Town of Caswell Beach 1100 Caswell Beach Rd. c/o Mr. Jim Carter, Town Administrator Caswell Beach, N.C. 28465
More informationLABORATORY 10 SOIL FERTILITY. Objectives
LABORATORY 10 SOIL FERTILITY I Objectives Learn a method for measuring extractable soil P. Make fertilizer recommendations for a crop. Gain experience with calculations involving inorganic fertilizer materials.
More informationActivity 1: Using the Level of Oxygen- Saturation Chart graph Dissolved Oxygen vs. Temperature at 100% saturation. Start by making a 2 column table.
Activity 1: Using the Level of Oxygen- Saturation Chart graph Dissolved Oxygen vs. Temperature at 100% saturation. Start by making a 2 column table. Number the first column with temperatures from 0 to
More informationRunoff and Its Impact on the Ecosystem
Runoff and Its Impact on the Ecosystem We are looking at the effects that soil nutrients in runoff, specifically nitrogen, have on bacteria in the soil. Before we started our experiment, we researched
More informationLos Angeles Fresh Water Testing LAB
Los Angeles Fresh Water Testing LAB Background: Freshwater is placed in Los Angeles from precipitation. The water either goes into the ground (which percolates into groundwater reservoirs) or runs off
More informationRayBio Caspase-3 Colorimetric Assay Kit
RayBio Caspase-3 Colorimetric Assay Kit User Manual Version 1.0 Mar 25, 2013 Caspase-3 Colorimetric Assay Kit Protocol Catalog numbers: 68CL-Casp3-S25 68CL-Casp3-S100 68CL-Casp3-S200 68CL-Casp3-S400 RayBiotech,
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationCytoScan WST 1 Cell Cytotoxicity Assay
087PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name CytoScan WST 1 Cell Cytotoxicity Assay Colorimetric Assay for Quantitation of Cellular
More informationSwab and Samplers Test Kits Quality control as easy as 1, 2, 3
Swab and Samplers Test Kits Quality control as easy as 1, 2, 3 Merck Millipore is a division of Environmental monitoring Sample, incubate, count Merck Millipore s Samplers and Swab Test Kits simplify routine
More informationLecture 7 Water Quality Monitoring: Estimation of fecal indicator bacteria
Lecture 7 Water Quality Monitoring: Estimation of fecal indicator bacteria FECAL INDICATOR BACTERIA Fecal indicator bacteria, which are directly associated with fecal contamination, are used to detect
More informationENVR 421 Laboratory #1: Basic Bacteriology Techniques
ENVR 421 Laboratory #1: Basic Bacteriology Techniques Introduction The purpose of this laboratory exercise is to familiarize you with two fundamental bacteriology techniques: the streak plate and the spread
More informationLab Exercise 13: Growth Curve
Lab Exercise 13: Growth Curve OBJECTIVES 1. Know the different phases of a standard growth curve. 2. Understand and perform direct measurement of bacterial growth through serial dilutions and standard
More informationMr. Leach Science Burrillville High School
Mr. Leach Science Burrillville High School The Clear River subwatershed, is located in northwestern Rhode Island. This subwatershed is a component of the Lower Blackstone River Watershed, the Blackstone
More informationThe goal of our experiment was to see how a temperature above or below the
Abstract: The goal of our experiment was to see how a temperature above or below the optimum temperature for the growth of Escherichia coli (E. coli) affected the growth rate. We decided to address this
More informationSIERRA CLUB. Protect America s Environment. For our Families For our Future. 85 Second Street San Francisco, California 94105
SIERRA CLUB Protect America s Environment. For our Families For our Future 85 Second Street San Francisco, California 94105 SAFE DRINKING WATER IS ESSENTIAL FOR HEALTHY COMMUNITIES WATER POLLUTION IS A
More informationENV H 433 LABORATORY EXERCISE 1
ENV H 433 LABORATORY EXERCISE 1 Multiple tube fermentation and presence/absence methods to detect total coliforms, fecal coliforms and Escherichia coli I. LABORATORY GOAL To determine concentration of
More informationToday is your last chance to turn in the short topic questionnaire!
In the last class you: Heard about the Water Use Cycle as it relates to this course. We also discussed units for quantifying water quality parameters (ex., mg/l and ppm), and equivalents/l. There were
More informationGuidelines for the Reduction of Dyes Released from Pulp and Paper Mills. Environment Canada
Guidelines for the Reduction of Dyes Released from Pulp and Paper Mills Environment Canada July 2012 Table of Contents Foreword... 3 Definitions... 4 Scope... 4 Performance guidelines... 5 Declaration...
More informationThe significance of the difference between the Escherichia coli numbers in six different brands of 19 liters reusable plastic bottles of water
The significance of the difference between the Escherichia coli numbers in six different brands of 19 liters reusable plastic bottles of water Is there any difference in terms of the amount (colony number
More informationMicrobiological Quality: Understanding Drinking Water Quality and Management
S A S K A T C H E W A N Microbiological Quality: Understanding Drinking Water Quality and Management June 2017 EPB 197 Table of Contents Page Number How to Determine the Microbiological Quality... 1 Monitoring
More informationPortland Water District Sebago Lake Watershed Monitoring Programs Crooked River Monitoring (1977 to 2017) Brie Holme
Portland Water District Sebago Lake Watershed Monitoring Programs Crooked River Monitoring (1977 to 2017) Brie Holme Introduction Sebago Lake is the primary drinking water supply for 200,000 people in
More informationCollege of the Canyon s Introduction to Biotechnology Course, Custom Lab
College of the Canyon s Introduction to Biotechnology Course, Custom Lab Protein Extraction and Concentration Determination Version 7-22-12 The standard curve that you created in the protein standard curve
More informationRayBio Caspase Colorimetric Substrate Set II Plus
RayBio Caspase Colorimetric Substrate Set II Plus User Manual Version 1.0 Mar 25, 2014 RayBio Caspase Colorimetric Substrate (Cat#: 68FL-CaspP-S925) RayBiotech, Inc. We Provide You With Excellent Support
More informationILLICIT DISCHARGE INDICATOR THRESHOLDS MEMORANDUM
ILLICIT DISCHARGE INDICATOR THRESHOLDS MEMORANDUM Prepared for Washington State Department of Ecology Prepared by Herrera Environmental Consultants, Inc. Note: Some pages in this document have been purposely
More informationCOMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Biomaterial Hemolytic Assay. Kit Insert
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Biomaterial Hemolytic Assay Kit Insert Version: April 2017 Summary Hemolytic activity is a requirement to be tested for any blood
More informationIron Assay Kit. Catalog Number KA assays Version: 05. Intended for research use only.
Iron Assay Kit Catalog Number KA0814 100 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials Supplied...
More informationc: Don Labossiere, Director, Environmental Compliance and Enforcement Public Registries
Climate Change and Environmental Protection Division Environmental Approvals Branch 123 Main Street, Suite 160, Winnipeg, Manitoba R3C 1A5 T 204 945-8321 F 204 945-5229 www.gov.mb.ca/conservation/eal CLIENT
More informationGreener Homes National Summit Rainwater Harvesting & Rain Garden Design and Performance Sustainable Water Management Session September
Greener Homes National Summit Rainwater Harvesting & Rain Garden Design and Performance Sustainable Water Management Session September 28 2011 Ty Newell Professor Emeritus Mechanical Science and Engr University
More informationColiform bacteria are quantitated by the fractional gram pour plate technique (Note 1). Test tubes containing gas collector tubes (Durham Tubes)
Microbiological Methods IV-A- 1 (STANDARD PLATE COUNT METHOD) PRINCIPLE SCOPE Coliform bacteria are quantitated by the fractional gram pour plate technique (Note 1). The method is applicable to starches,
More informationPortland Water District Sebago Lake Watershed Monitoring Programs Crooked River Monitoring (1977 to 2016) Brie Holme
Portland Water District Sebago Lake Watershed Monitoring Programs Crooked River Monitoring (1977 to 2016) Brie Holme Introduction Sebago Lake is the primary drinking water supply for 200,000 people in
More informationRainwater Harvesting: The Relationship Between Water Supply Contamination and Community Participation in Rural Karnataka, India
University of Massachusetts Amherst ScholarWorks@UMass Amherst Water Resources Research Center Conferences Water Resources Research Center 4-9-2007 Rainwater Harvesting: The Relationship Between Water
More informationWater Pollution Overview. Sewage dumping
Water Pollution Water Pollution Overview Sewage dumping Ocean Pollution Water Pollution Overview Urban Water Pollution Water Pollution Overview Water Pollution Overview Urban Runoff Water Pollution Overview
More informationEach group or student should have a worksheet.
Testing the Water Quality of the Manoa Stream Lesson 9 --------------------------------------------------------------------------------------------------------------------- Objectives The student will
More information-ECVAM Skin Irritation Validation Study-
Version : 1.2 Page 1 of 16 September 2005 EPIDERMIS MODEL EPISKIN -ECVAM Skin Irritation Validation Study- VALIDATION OF THE EPISKIN SKIN IRRITATION TEST - 42 HOURS ASSAY FOR THE PREDICTION OF ACUTE SKIN
More informationIDEXX is an ISO certified facility. Gil Dichter World Wide Technical Support Manager
USEPA ATP Process for Approval of Colilert-18 and Quanti-Tray for the Detection of Fecal Coliforms in Waste Water; What is a Number: Comparison of MPN and CFU Gil Dichter World Wide Technical Support Manager
More informationImpacts of Rainfall Events on Water Quality in the Houston Metro Area
Impacts of Rainfall Events on Water Quality in the Houston Metro Area Hanadi Rifai and Anuradha Desai Civil and Environmental Engineering University of Houston, Houston, TX Acknowledgments TCEQ EPA Houston
More informationMEMORANDUM Project Specific:
Information Only MEMORANDUM Project Specific: 5-829-007 Policy Memo - File With TO: Robert Flaherty, Bullitt County Deputy Judge Executive FROM: Adam Connelly DATE: RE: 2006 Wet Weather Water Quality Monitoring
More informationSerial dilution and colony count (Viable count) Pour plate. Spread plate Membrane filtration. Turbidity. Microscopic cell count
Aljawharah Alabbad 2016 Serial dilution and colony count (Viable count) Pour plate Spread plate Membrane filtration Turbidity Microscopic cell count Many studies require the quantitative determination
More informationOriginal Research Paper
40 International Journal of Tropical Medicine and Public Health Volume 1, Issue 1, 2011 Crosshouse books Original Research Paper EFFICACY OF SAND FILTRATION, Moringa oleifera SEED AND ALUM TREATMENT IN
More informationLarge Volume Serial Dilutions:
Serial Dilutions All three bacterial plate count methods described in lab require you to serially dilute your samples until you have 30-300 colony forming units (CFU) on the plate. Plates with more than
More informationFor the rapid, sensitive and accurate measurement of Caspase 3 Activity in cell and tissue lysates.
ab39401 Caspase 3 Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Caspase 3 Activity in cell and tissue lysates. This product is for research use only
More informationHow Ecosystems Work Section 2
Objectives List the three stages of the carbon cycle. Describe where fossil fuels are located. Identify one way that humans are affecting the carbon cycle. List the tree stages of the nitrogen cycle. Describe
More informationSoil Ecology Project. The Effect of Road Salt on Bacteria Density. Hope Duke, Irina Kolesnik and Clare O Brien Mr. Brock Biology 9 Honors
Soil Ecology Project The Effect of Road Salt on Bacteria Density Hope Duke, Irina Kolesnik and Clare O Brien Mr. Brock Biology 9 Honors 5/31/2012 A. Background Report Soil is made out of four basic components:
More informationBIO BASIC INC. BRADFORD PROTEIN ASSAY KIT SK3031. Version 4.0 ISO9001 Certified
BIO BASIC INC. BRADFORD PROTEIN ASSAY KIT SK3031 Version 4.0 ISO9001 Certified 20 Konrad Crescent, Markham, Ontario L3R 8T4 Canada Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794 Email: order@biobasic.com
More information2009 Summary Report for Municipalities
2009 Summary Report for Municipalities (As per Schedule 22 of O. Reg. 170/03) The Onaping Potable Water System - #220003519 Certificate of Approval 1414-0696HD3 0014-6MQQFQ Prepared by: Jean-Marc Joliat
More informationNucleic acid-free silica-matrix: Regeneration of DNA binding columns
MAXXBOND ready-to-use - Kit for the regeneration of DNA binding columns with pure silica matrices Product No. MB007 Nucleic acid-free silica-matrix: Regeneration of DNA binding columns efficient and easy
More informationPLASMID DNA EXTRACTION FROM BACTERIAL CELLS 1
PLASMID DNA EXTRACTION FROM BACTERIAL CELLS 1 Practical Two: Plasmid DNA Extraction from Bacterial Cells Student s Name University Affiliate PLASMID DNA EXTRACTION FROM BACTERIAL CELLS 2 Aims The purpose
More informationLDH-Cytotoxicity Assay Kit II
LDH-Cytotoxicity Assay Kit II Catalog Number KA0786 500 assays Version: 08 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationtill /Mission Water & Sewer Services REPORT
tr '! Abbotsfo rd till /Mission Water & Sewer Services REPORT To: From: Abbotsford/Mission Water & Sewer Commission (WSC) Len Stein, Director of Utility Operations Date: June 29, 2010 Subject: JAMES Plant
More informationNATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA. National Food Safety Standard
GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 食品安全国家标准食品微生物学检验大肠埃希氏菌计数
More informationFARM MICROBIOLOGY 2008 PART 7: WATER & WASTEWATER MICROBIOLOGY. B. The water supply and the hydrologic cycle.
FARM MICROBIOLOGY 2008 PART 7: WATER & WASTEWATER MICROBIOLOGY I. Water General and Microbiology. A. Domestic use of water. Drinking, bathing, cleaning, formulating drugs, mediamaking (for bacteriology
More informationCat # Box1 Box2. DH5a Competent E. coli cells CCK-20 (20 rxns) 40 µl 40 µl 50 µl x 20 tubes. Choo-Choo Cloning TM Enzyme Mix
Molecular Cloning Laboratories User Manual Version 3.3 Product name: Choo-Choo Cloning Kits Cat #: CCK-10, CCK-20, CCK-096, CCK-384 Description: Choo-Choo Cloning is a highly efficient directional PCR
More informationM. Dalbey/Bio 105M Isolation of E. coli - Isolation of E. coli from an Environmental Sample
Isolation of E. coli from an Environmental Sample We want to expand our horizons a bit beyond the domesticated lab strains of E. coli. In this exercise you will isolate "wild" E. coli strains from an environmental
More informationECO Smart Aerobic Waste Water Treatment System. Optimising the re-use and recycling of waste water
Optimising the re-use and recycling of waste water The ECO Smart aerobic wastewater treatment system is a selfcontained wastewater treatment system that utilizes a combination of anaerobic as well as aerobic
More informationThe Biotechnology Education Company. Water Quality Testing I: Chromogenic Analysis of Water Contaminants. See Page 3 for storage instructions.
The Biotechnology Education Company REVISED & UPDATED EDVO-Kit 951 Water Quality Testing I: Chromogenic Analysis of Water Contaminants See Page 3 for storage instructions. EXPERIMENT OBJECTIVE: The objective
More information