7 Preparative Columns

Transcription

1 YMC GENERAL CATALOG Overview of optimization methods for isolation/purification YMC-Actus series YMC-Pack Pro C18 YMC-Pack R&D series YMC-Pack preparative columns ODS series C8 C4 TMS Ph CN SIL SIL-06 Polyamine NH2 Diol YMC-DispoPack 136, , , 第 0 章.indd :58:38 AM

2 Preparative packed columns Overview of optimization methods for isolation/purification An overview of the methods for selecting optimum conditions for isolation/purification and conducting efficient isolation, as well as points to check are given below. Selection of a preparative column: General comments 1)Selecting the separation mode of chromatography When more than one mode is available for the separation of samples, the following points should be considered for the selection of an appropriate separation mode: (1)Resolution:Selectivity of the packing material for the compound of interest (2)Load:Capacity of the packing material (3)Speed:Isolation time 2) The table shown in below provides a rough guide for selection of column inner diameters and packing material particle sizes. (1)Column inner diameter:sample load is proportional to the column cross-sectional area. It is necessary to select a column inner diameter suitable for the sample load. (2)Packing material particle size:smaller particle sizes result in higher column efficiency, however, which also results in higher prices and higher column pressure. In addition, the equipment used needs to be resistant to the pressure. When the target component and the nearest peak are very near and the highest resolution is needed, packing materials with small particle size are useful. In contrast, larger particle sizes result in lower column efficiency, but result in lower prices and lower column pressure. (3)Column length:longer column lengths result in higher resolution and higher sample load, but the column pressure becomes larger and the separation time longer. 136 第 0 章.indd :58:41 AM

3 Steps for Performing a Preparative Separation 1) Consider screening packing materials that can be scaled for preparative isolations. If you suspect that larger quantities of the compound needing purification will be required in the future, consider performing your analytical scale investigations on a packing material that is available in preparative particle sizes (10 micron and larger). As the requirements for the purified product become greater at later stages of the project, you will have the option to isolate larger quantities of material on larger particle sizes in larger columns on the same packing chemistry. This is an important consideration if the compound should be required in much larger quantities as the project matures. 2) Perform selectivity studies on analytical scale columns (4.6 mm I.D. and smaller) on a variety of packing materials under several sets of conditions. Automated software programs may be particularly helpful in predicting the most desirable separation modes and in helping to choose the best chromatographic conditions for your separations in the shortest amount of time. 3) Once the best resolution is obtained, perform loading studies on the analytical scale column. Evaluate product purity at variable loadings and select the maximum load allowed for a desired product purity. 4) Select the size of preparative column that will be needed by scaling up the separation based on the loading obtained on the analytical column. If possible, use the same particle size and column length to achieve predictable preparative results. Scale up the loading of the preparative column based on the ratio of the cross-sectional areas of the preparative and anayltical columns. 5) Perform the preparative separation and evaluate the yield and purity. 13 第 0 章.indd :58:42 AM

4 High durability semi-preparative columns YMC-Actus series Axial Compression Technology for Ultimate Separation YMC-Actus series are semi-preparative HPLC columns that have excellent column durability and efficiency by applying axial compression technology. YMC-Actus series columns show high durability under high flow rate or steep gradient conditions and desirable for milligram scale preparative HPLC of various compounds. Excellent efficiency and durability preparative HPLC column Features Improved durability by applying axial compression technology Prepacked column for milligram scale preparative HPLC Excellent resolution Applying axial compression technology Slurry packing Image of YMC Actus 10% increase in bed density With compression Attaching the end assembly Compressing After the stability test under high-speed and high-pressure Flow No change Test conditions (high-speed and high-pressure) :50 20 mmi.d. 5 µm Eluent :A)water B)methanol Gradient :5-95% B Flow rate :50 ml/min Pressure : 1 MPa Image of conventional column Without compression Flow after 00 run initial Void and crack Degradation of performance after 300 run initial Uniformly high density packing is necessary for high efficiency and high durability of HPLC column. DAC (Dynamic Axial Compression) column is widely used for preparative separation in pilot or production scale. It allows uniformly high density packing and prevents formation of voids. YMC-Actus series have been developed by applying this Axial Compression Technology to semi-prep column. The column bed is compressed adequately by attaching end assembly newly designed for YMC-Actus. It provides proper bed density (10% higher than conventional columns) and bed uniformity. 138 第 0 章.indd :58:45 AM

5 Outstanding durability and efficiency YMC-Actus Brand l6 Conventional Preparative Column Sequential gradient test 100 run Column performance test Sequential gradient test (high-speed and high-pressure) : mmi.d. 5 µm or mmi.d. 5 µm Eluent :A) water B) methanol Gradient : 5% B (0-0.5 min), 5-95% B ( min), 95% B ( min), 5% B ( min) Flow rate :50 ml/min Column performance test : mmi.d. 5 µm or mmi.d. 5 µm Eluent :methanol/water (60/40) Flow rate :10 ml/min In case of wrong packing conditions, rapid pressure change under high-speed gradient condition degrades column performance. To examine bed stability, the column performance was evaluated after every 100 run of the sequential high-speed gradient. YMC-Actus offers superior initial column efficiency to competitors. Furthermore, its excellent performance is maintained after a long series of gradient tests. Available for high-throughput purification: injection in DMSO mau Ethenzamide 2. Clemastine fumarate 3. Ibuprofen th 800th 600th 400th 200th 1st min Column : YMC-Actus Pro C18 RS (5 µm, 80 Å) mmi.d. Eluent : A) water/tfa (100/0.1) B) acetonitrile/tfa (100/0.1) 10-90% B (0-3 min), 90% B (3-5 min), 10% B (5-10 min) Flow rate :20 ml/min Temperature :ambient Detection :UV at 20 nm Injection :100 µl (0.2-2 mg/ml in DMSO) As shown in overlay chromatograms, YMC-Actus provides outstanding stability and reproducibility in the separation of pharmaceuticals dissolved in 100% DMSO, even after 1,000 injections under the test conditions. It is ideal for high-throughput purification in drug discovery. Purification of highly polar compounds -oligonucleotide- Crude synthetic 30 mer oligonucleotide 5'-CCGCTCGAGCTAAAAAAAGCCTGTGTTACC-3' Impurities 30 mer Eluent : A) 10 mm DBAA* (ph 6.0)/methanol (60/40) B) 10 mm DBAA* (ph 6.0)/methanol (20/80) 10-35% B (0-30 min) Temperature :ambient Detection :UV at 269 nm Sample :synthetic oligonucleotide (100 µm) * di-n-butylammonium acetate min min Impurities 30 mer min min In analytical scale, many impurities could be separated from the target compound by onenucleotide difference on Hydrosphere C18. Even in purification scale, YMC-Actus gave superior separation and recovery. YMC-Actus Hydrosphere C18 is useful for purification of hydrophilic compounds such as oligonucleotides, organic acids, oligosaccharides and glycosides. 139 第 0 章.indd :58:48 AM

6 High durability semi-preparative columns YMC-Actus series Purification of hydrophobic compounds with similar structure -Capsaicinoids in red pepper min min min Capsaicin 2 3 Purity > 99.9% min 1 H3CO HO 2 H3CO HO N H Nordihydrocapsaicin N H O O Capsaicin CH3 CH3 CH3 CH3 1 3 H3CO N H O CH3 HO CH min Dihydrocapsaicin Eluent : A) methanol/water/tfa (50/50/0.1) B) methanol/tfa (100/0.1) 10-30% B (0-5 min), 30% B (5-10 min) Temperature : 25 for mmi.d. ambient for mmi.d. Detection :UV at 280 nm Sample : methanol extract of a commercial cayenne pepper (1 g cayenne pepper/3 ml) Pro C18 RS has superior selectivity for hydrophobic compounds that differ slightly in structure and hydrophobicity, achieves better resolution between peak 1 and peak 2. Furthermore, analytical separation can be directly scaled up to preparative scale with YMC-Actus Pro C18 RS. YMC-Actus series have high resolution equal to analytical columns. Purification of crude extract under fast gradient condition at high flow rate -Glycyrrhizic acid in herb medicine- Glycyrrhizic acid Purity 99.5% Glycyrrhizic acid min min min Glycyrrhizic acid Eluent : A) water/acetic acid (99/1) B) methanol/acetic acid (99/1) 20% B (0-2 min), 20-45% B (2- min), 45% B (-10 min) Temperature :ambient Detection :UV at 260 nm Sample : methanol/water/acetic acid extract of commercially available herb medicine (0.1 g/ml) In high-speed conditions, target product is separated from many impurities within a short time. Also in purification scale, glycyrrthizic acid with high purity is obtained on YMC-Actus ODS-AQ. YMC-Actus series are developed for applicable to a high-throughput gradient condition and show excellent column stability and efficiency. YMC-Actus series are useful for screening or isolation in the field of combinatorial chemistry. 140 第 0 章.indd :58:52 AM

7 Abundant packing material types are available Packing material pore size particle size C% Usable ph range Characteristics Triart C ~12.0 Pro series Pro C Hydrosphere C ~ 8.0 Pro C18 RS ~ 10.0 Pro C ~.5 YMC-Pack series ODS-A ~.5 Versatile hybrid silica based ODS packing material Great chemical durability Excellent resolution in every condition High performance ODS packing material Can be used with 100% water mobile phase Superior separation for hydrophilic compounds High carbon ODS packing material, high durability Processed with advanced endcapping technology Superior separation of basic compounds Standard ODS from analytical to preparative ODS-AQ Good separation for hydrophilic compounds High durability semi-preparative columns Packing material Pose size Triart C18 S TA12S WX TA12S WX TA12S WX 30 5 TA12S05-L530WX TA12S WX Pro C18 S AS12S WX AS12S WX AS12S WX 30 5 AS12S05-L530WX AS12S WX Hydrosphere C18 Pro C18 RS S HS12S WX HS12S WX HS12S WX 30 5 HS12S05-L530WX HS12S WX S RS08S WX RS08S WX RS08S WX 30 5 RS08S05-L530WX RS08S WX Packing material Pose size Pro C8 S OS12S WX OS12S WX OS12S WX 30 5 OS12S05-L530WX OS12S WX ODS-A S AA12S WX AA12S WX AA12S WX 30 5 AA12S05-L530WX AA12S WX ODS-AQ S AQ12S WX AQ12S WX AQ12S WX 30 5 AQ12S05-L530WX AQ12S WX Other than listed above, inquire us. 141 第 0 章.indd :58:53 AM

8 YMC-Pack Pro C18 Superior separation of basic compounds Silica gel base, low in metal impurities Two types of test reports are included with each column :120 Å Carbon content:16% Usable ph range: H i g h p e r f o r m a n c e p a c k i n g m a t e r i a l f o r p r e p a r a t i v e i s o l a t i o n YMC-Pack Pro C18 is available in 5 and 10 micron particles in preparative column size. Chromatograms of Coptis Rhizome (Japanese Goldthread) min Column : mmi.d. Eluent :methanol/thf/10 mm CH3COONH4 (15/2/5) Flow rate :2.0 ml/min Temperature :40 Detection :UV at 254 nm Sample :Coptis Rhizome (Japanese Goldthread) min min YMC-Pack Pro C18 is processed with advanced endcapping technology, resulting in good peak shapes. S AS12S WT AS12S WT AS12S WT 30 5 AS12S05-L530WT AS12S WT AS12S WT S AS12S WT AS12S WT AS12S WT AS12S WT AS12S WT AS12S WT AS12S WT AS12S DR AS12S DR S AS12S WTG AS12S WTG S AS12S WTG AS12S WTG AS12S DRG DR=for 1/8 tubing. For 1/16 tubing, please ask. 142 第 0 章.indd :58:55 AM

9 Isolation of berberine O O Analysis of the preparative fraction CH 3 O OCH 3 N + Cl - Berberine 0 10 min min Column :YMC-Pack Pro C18 (5 µm, 120 Å) mml.d. Eluent :acetonitrile/50 mm KH2PO4 (50/50) containing 6 mm SDS Flow rate :1.0 ml/min Temperature :40 Detection :UV at 254 nm yield 88% min min Column :YMC-Pack Pro C18 Eluent :methanol/thf/10 mm CH3COONH4 (15/2/5) Temperature :40 Detection :UV at 254 nm 143 第 0 章.indd :58:58 AM

10 YMC-Pack R&D series A s e m i - p r e p a r a t i v e c o l u m n a n d a n a n a l y t i c a l c o l u m n s e t YMC-Pack R&D series are packaged column sets that include a semi-preparative column and an analytical column packed with high quality YMC*GEL. The packing materials used for the semi-preparative column and the analytical column are from the same lots and the selectivity is identical. Analytical conditions can be directly scaled up and can be reproduced with the preparative column and the preparative separation can be conducted simply and rapidly. R&D set Application example Isolation of terpene Column Analytical conditions YMC-Pack ODS-A/R, 5 µm, 120 Å mmi.d. Preparative conditions YMC-Pack ODS-A/D, 5 µm, 120 Å mmi.d. Eluent acetonitrile/water (65/35) Flow rate 1.0 ml/min 9.9 ml/min Pressure 12 MPa 18 MPa Detection RI RIU/FS Injection 50 µl (1 mg) 1 ml (20 mg) min Packing material min for analysis(r) for preparative(d) ODS-A ( 120 Å) S AA12S05-RD AA12S05-RD AA12S05-RD2 S AA12S11-RD AA12S11-RD2 S AA12S16-RD AA12S16-RD2 SIL ( 120 Å) S SL12S05-RD SL12S05-RD SL12S05-RD2 S SL12S11-RD SL12S11-RD2 S SL12S16-RD SL12S16-RD2 SIL SL06S05-RD06 ( 60 Å) SL06S05-RD SL06S05-RD2 S SL06S11-RD SL06S11-RD2 S SL06S16-RD SL06S16-RD2 PolyamineⅡ S PB12S05-RD0 PROTEIN-RP S PR99S05-RD0 144 第 0 章.indd :59:01 AM

11 YMC-Pack ODS-A Standard ODS YMC-Pack ODS-A S AA12S WT AA12S WT AA12S WT 30 5 AA12S05-L530WT AA12S WT AA12S WT AA12S WT AA12S DR AA20S WT AA20S WT AA20S WT AA20S DR AA30S WT AA30S WT AA30S WT AA30S DR S AA12S WT AA12S WT AA12S WT AA12S WT AA12S WT AA12S DR AA12S DR AA20S WT AA20S WT AA20S WT AA20S WT AA20S WT AA20S DR AA20S DR AA30S WT AA30S WT AA30S WT AA30S WT AA30S WT AA30S DR AA30S DR S AA12S WT AA12S WT AA12S WT AA12S WT AA12S WT AA12S DR AA12S DR AA20S WT AA20S WT AA20S WT AA20S WT AA20S WT AA20S DR AA20S DR AA30S WT AA30S WT AA30S WT AA30S WT AA30S WT AA30S DR AA30S DR S AA12S WT AA12S WT AA12S WT AA12S WT AA12S DR AA12S DR S AA20S WT AA20S WT AA20S WT AA20S WT AA20S DR AA20S DR AA30S WT AA30S WT AA30S WT AA30S WT AA30S DR AA30S DR S AA12S WT AA12S WT AA12S WT AA12S WT AA12S DR AA12S DR S AA12S WTG AA12S WTG AA12S DRG AA20S WTG AA20S WTG AA20S DRG AA30S WTG AA30S WTG AA30S DRG S AA12S WTG AA12S WTG AA12S DRG AA20S WTG AA20S WTG AA20S DRG AA30S WTG AA30S WTG AA30S DRG S AA12S WTG AA12S WTG AA12S DRG AA20S WTG AA20S WTG AA20S DRG AA30S WTG AA30S WTG AA30S DRG S AA12S WTG AA12S WTG AA12S DRG AA20S WTG AA20S WTG AA20S DRG AA30S WTG AA30S WTG AA30S DRG S AA12S WTG AA12S WTG AA12S DRG 145 第 0 章.indd :59:02 AM

12 YMC-Pack ODS-AQ ODS useful for separation of hydrophilic compounds YMC-Pack ODS-AQ S AQ12S WT AQ12S WT AQ12S WT 30 5 AQ12S05-L530WT AQ12S WT AQ12S WT AQ12S WT AQ12S DR AQ20S WT AQ20S WT AQ20S WT AQ20S DR S AQ12S WT AQ12S WT AQ12S WT AQ12S WT AQ12S WT AQ12S DR AQ12S DR AQ20S WT AQ20S WT AQ20S WT AQ20S WT AQ20S WT AQ20S DR AQ20S DR S AQ12S WT AQ12S WT AQ12S WT AQ12S WT AQ12S WT AQ12S DR AQ12S DR AQ20S WT AQ20S WT AQ20S WT AQ20S WT AQ20S WT AQ20S DR AQ20S DR S AQ12S WT AQ12S WT AQ12S WT AQ12S WT AQ12S DR AQ12S DR S AQ12S WT AQ12S WT AQ12S WT AQ12S WT AQ12S DR AQ12S DR S AQ12S WTG AQ12S WTG AQ12S DRG AQ20S WTG AQ20S WTG AQ20S DRG S AQ12S WTG AQ12S WTG AQ12S DRG AQ20S WTG AQ20S WTG AQ20S DRG S AQ12S WTG AQ12S WTG AQ12S DRG AQ20S WTG AQ20S WTG AQ20S DRG S AQ12S WTG AQ12S WTG AQ12S DRG S AQ12S WTG AQ12S WTG AQ12S DRG 146 第 0 章.indd :59:04 AM

13 YMC-Pack ODS-AM ODS with high reproducibility YMC-Pack ODS-AL ODS with residual silanol groups YMC-Pack ODS-AM S AM12S WT AM12S WT AM12S WT 30 5 AM12S05-L530WT AM12S WT AM12S WT AM12S WT AM12S DR S AM12S WTG AM12S WTG AM12S DRG YMC-Pack ODS-AL S AL12S WT AL12S WT AL12S WT 30 5 AL12S05-L530WT AL12S WT AL12S WT AL12S WT AL12S DR S AL12S WTG AL12S WTG AL12S DRG 14 第 0 章.indd :59:05 AM

14 YMC-Pack C8 Characterized by moderate hydrophobicity YMC-Pack C8 S OC12S WT OC12S WT OC12S WT 30 5 OC12S05-L530WT OC12S WT OC12S WT OC12S WT OC12S DR OC20S WT OC20S WT OC20S WT OC20S DR OC30S WT OC30S WT OC30S WT OC30S DR S OC12S WT OC12S WT OC12S WT OC12S WT OC12S WT OC12S DR OC12S DR OC20S WT OC20S WT OC20S WT OC20S WT OC20S WT OC20S DR OC20S DR OC30S WT OC30S WT OC30S WT OC30S WT OC30S WT OC30S DR OC30S DR S OC12S WT OC12S WT OC12S WT OC12S WT OC12S WT OC12S DR OC12S DR OC20S WT OC20S WT OC20S WT OC20S WT OC20S WT OC20S DR OC20S DR S OC12S WT OC12S WT OC12S WT OC12S WT OC12S DR OC12S DR S OC20S WT OC20S WT OC20S WT OC20S WT OC20S DR OC20S DR OC30S WT OC30S WT OC30S WT OC30S WT OC30S DR OC30S DR S OC12S WTG OC12S WTG OC12S DRG OC20S WTG OC20S WTG OC20S DRG OC30S WTG OC30S WTG OC30S DRG S OC12S WTG OC12S WTG OC12S DRG OC20S WTG OC20S WTG OC20S DRG OC30S WTG OC30S WTG OC30S DRG S OC12S WTG OC12S WTG OC12S DRG OC20S WTG OC20S WTG OC20S DRG S OC12S WTG OC12S WTG OC12S DRG OC20S WTG OC20S WTG OC20S DRG OC30S WTG OC30S WTG OC30S DRG 148 第 0 章.indd :59:06 AM

15 YMC-Pack C4 Reversed-phase column with shorter alkyl chain YMC-Pack C4 S BU12S WT BU12S WT BU12S WT 30 5 BU12S05-L530WT BU12S WT BU12S WT BU12S WT BU12S DR BU20S WT BU20S WT BU20S WT BU20S DR BU30S WT BU30S WT BU30S WT BU30S DR S BU12S WT BU12S WT BU12S WT BU12S WT BU12S WT BU12S DR BU12S DR BU20S WT BU20S WT BU20S WT BU20S WT BU20S WT BU20S DR BU20S DR BU30S WT BU30S WT BU30S WT BU30S WT BU30S WT BU30S DR BU30S DR S BU12S WT BU12S WT BU12S WT BU12S WT BU12S WT BU12S DR BU12S DR BU20S WT BU20S WT BU20S WT BU20S WT BU20S WT BU20S DR BU20S DR BU30S WT BU30S WT BU30S WT BU30S WT BU30S WT BU30S DR BU30S DR S BU12S WT BU12S WT BU12S WT BU12S WT BU12S DR BU12S DR S BU20S WT BU20S WT BU20S WT BU20S WT BU20S DR BU20S DR BU30S WT BU30S WT BU30S WT BU30S WT BU30S DR BU30S DR S BU12S WTG BU12S WTG BU12S DRG BU20S WTG BU20S WTG BU20S DRG BU30S WTG BU30S WTG BU30S DRG S BU12S WTG BU12S WTG BU12S DRG BU20S WTG BU20S WTG BU20S DRG BU30S WTG BU30S WTG BU30S DRG S BU12S WTG BU12S WTG BU12S DRG BU20S WTG BU20S WTG BU20S DRG BU30S WTG BU30S WTG BU30S DRG S BU12S WTG BU12S WTG BU12S DRG BU20S WTG BU20S WTG BU20S DRG BU30S WTG BU30S WTG BU30S DRG 149 第 0 章.indd :59:0 AM

16 YMC-Pack TMS Reversed-phase column with the lowest hydrophobicity YMC-Pack Ph Reversed-phase column with π electrons YMC-Pack TMS S TM12S WT TM12S WT TM12S WT 30 5 TM12S05-L530WT TM12S WT TM12S WT TM12S WT TM12S DR S TM12S WT TM12S WT TM12S WT TM12S WT TM12S WT TM12S DR TM12S DR S TM12S WT TM12S WT TM12S WT TM12S WT TM12S WT TM12S DR TM12S DR S TM12S WT TM12S WT TM12S WT TM12S WT TM12S DR TM12S DR S TM12S WTG TM12S WTG TM12S DRG S TM12S WTG TM12S WTG TM12S DRG S TM12S WTG TM12S WTG TM12S DRG S TM12S WTG TM12S WTG TM12S DRG YMC-Pack Ph S PH12S WT PH12S WT PH12S WT 30 5 PH12S05-L530WT PH12S WT PH12S WT PH12S WT PH12S DR S PH12S WT PH12S WT PH12S WT PH12S WT PH12S WT PH12S DR PH12S DR S PH12S WT PH12S WT PH12S WT PH12S WT PH12S WT PH12S DR PH12S DR S PH12S WT PH12S WT PH12S WT PH12S WT PH12S DR PH12S DR S PH12S WTG PH12S WTG PH12S DRG S PH12S WTG PH12S WTG PH12S DRG S PH12S WTG PH12S WTG PH12S DRG S PH12S WTG PH12S WTG PH12S DRG 150 第 0 章.indd :59:09 AM

17 YMC-Pack CN Column can be used in both normal-phase and reversed-phase modes YMC-Pack CN S CN12S WT CN12S WT CN12S WT 30 5 CN12S05-L530WT CN12S WT CN12S WT CN12S WT CN12S DR CN30S WT CN30S WT CN30S WT CN30S DR S CN12S WT CN12S WT CN12S WT CN12S WT CN12S WT CN12S DR CN12S DR S CN12S WT CN12S WT CN12S WT CN12S WT CN12S WT CN12S DR CN12S DR S CN12S WT CN12S WT CN12S WT CN12S WT CN12S DR CN12S DR S CN12S WTG CN12S WTG CN12S DRG CN30S WTG CN30S WTG CN30S DRG S CN12S WTG CN12S WTG CN12S DRG S CN12S WTG CN12S WTG CN12S DRG S CN12S WTG CN12S WTG CN12S DRG 151 第 0 章.indd :59:10 AM

18 YMC-Pack SIL YMC-Pack SIL-06 Standard normal-phase column YMC-Pack SIL S SL12S WT SL12S WT SL12S WT 30 5 SL12S05-L530WT SL12S WT SL12S WT SL12S WT SL12S DR S SL12S WT SL12S WT SL12S WT SL12S WT SL12S WT SL12S DR SL12S DR S SL12S WT SL12S WT SL12S WT SL12S WT SL12S WT SL12S DR SL12S DR S SL12S WT SL12S WT SL12S WT SL12S WT SL12S DR SL12S DR S SL12S WT SL12S WT SL12S WT SL12S WT SL12S DR SL12S DR YMC-Pack SIL-06 S SL06S WT SL06S WT SL06S WT 30 5 SL06S05-L530WT SL06S WT SL06S WT SL06S WT SL06S DR S SL06S WT SL06S WT SL06S WT SL06S WT SL06S DR S SL06S WT SL06S WT SL06S WT SL06S WT SL06S DR S SL06S WTG SL06S WTG SL06S DRG S SL06S WTG SL06S WTG SL06S DRG S SL06S WTG SL06S WTG SL06S DRG S SL12S WTG SL12S WTG SL12S DRG S SL12S WTG SL12S WTG SL12S DRG S SL12S WTG SL12S WTG SL12S DRG S SL12S WTG SL12S WTG SL12S DRG S SL12S WTG SL12S WTG SL12S DRG 152 第 0 章.indd :59:11 AM

19 YMC-Pack Polyamine Ⅱ Amino column with improved durability YMC-Pack NH2 Normal-phase column utilizing amino groups YMC-Pack Polyamine Ⅱ S PB12S WT S PB12S WTG YMC-Pack NH2 S NH12S WT NH12S WT NH12S WT S NH12S WTG NH12S WTG YMC-Pack Diol Gel filtration chromatography column YMC-Pack Diol Packing material Diol-60 S DL06S WT DL06S WT Diol-120 S DL12S WT DL12S WT Diol-200 S DL20S WT DL20S WT Diol-300 S DL30S WT DL30S WT Packing material Diol-60 S DL06S WTG Diol-120 S DL12S WTG Diol-200 S DL20S WTG Diol-300 S DL30S WTG 153 第 0 章.indd :59:12 AM

20 Disposable prepacked column YMC-DispoPack Disposable type prepacked column for low pressure preparative chromatography Adaptable to any LPLC systems Luer-lock connections D i s p o s a b l e c o l u m n s f o r p r e p a r a t i v e c h r o m a t o g r a p h y YMC-DispoPack has been developed to meet customer's needs for high-throughout purification for synthesized compounds. It consists of original disposable polypropylene column with special irregular packing materials for preparative isolation. YMC*GEL are available for custom made packing. Specifications Product ph range Maximum pressure/mpa Characteristics SIL Totally porous silica gel packing material Standard normal-phase material NH Aminopropyl-bonded silica gel Diol Dihydroxypropyl-bonded silica gel Alternative selectivity to silica in normal-phase separation Product name Specification YMC-DispoPack SIL YMC-DispoPack NH2 YMC-DispoPack Diol TYPE 20 9 g/ 20 ml 30-pack DP02SLK06I5230 TYPE g/ 60 ml 12-pack DP06SLK06I5212 TYPE g/200 ml 6-pack DP20SLK06I5206 TYPE 20 8 g/ 20 ml 30-pack DP02NHK15I5230 TYPE g/ 60 ml 12-pack DP06NHK15I5212 TYPE g/200 ml 6-pack DP20NHK15I5206 TYPE 20 9 g/ 20 ml 30-pack DP02DLK15I5230 TYPE g/ 60 ml 12-pack DP06DLK15I5212 TYPE g/200 ml 6-pack DP20DLK15I 第 0 章.indd :59:14 AM