Water and Food Analytics

Transcription

1 Water and Food Analytics Test Kits, Instruments and Accessories Merck Millipore is a division of

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3 When analyzing water and food, trust plays a very important role. Only careful analysis using tests that are performed correctly and are delivering sound results will help to maintain and improve our quality of life. With ready-to-use tests and test systems from Merck, you can trust your results. After all, when it comes to fast and precise analysis, Merck has been the leading specialist for more than 100 years. Our broad product range covers everything from water analysis to the control of production process up to the measurement of special food parameters. With our high-quality visual test kits you ll achieve results easily and within minutes also in on-site analyses. Our test systems consist of optimally coordinated kits and measuring instruments for a wide range of application areas. All of our test kits for instrumental analysis are supplied with detailed certificates. With ready-to-use standards, you ll make your quality controls as easy and reliable as possible. 3

4 Content NEW Standard solutions (CRM) for photometric applications To attain reliable results in analytical quality control, users need standard solutions that are traceable to globally accepted primary standards. That's why we offer now diluted, certified standard solutions in varying concentrations. The analysis certificates for these standard solutions document all batch-specific and analysis-relevant data such as exact concentration and expanded measurement uncertainty. Most importantly, it provides users with the certitude that the diluted, certified standard solutions are traceable directly to primary standard reference materials from NIST. For more information see page

5 Page Parameters from A-Z Visual and instrumental test kits at a glance 6 ph test strips and papers ph determination made easy 20 Test strips Universal, fast and simple Merckoquant 26 Colorimetric and titrimetric test kits Variety and ease of use Aquamerck Microquant Aquaquant 36 Reflectometry Easy in-process monitoring Reflectroquant 56 Photometry Safety in water analysis Spectroquant 64 Turbidimetry Clear view in many waters Turbiquant 134 Quality Assurance Proven and verifiable results 146 Service Simply competent 152 5

6 Parameters from A-Z Visual and instrumental test kits at a glance What options do the Merck rapid tests have to offer for the determination of the concentration of individual parameters? The following table is designed to assist you in selecting the appropriate test for your requirements: Select the test parameter of your choice (arranged alphabetically) Find the measuring range that applies to your requirements and use the table to select the line of products suited to your work. Get all requested information on the selected product from the stated page of this brochure. There the Applications section will give you details on the areas for which the product in question has been tested and is suitable. While a product has not been tested for use in applications that are not listed, it is nonetheless possible to apply it in these areas. You can use the order number stated to order the product. Many other details and information on the individual products for example working instructions, certificates of analysis, and applications can be found by entering the first six digits of the ordering number into the search box of our internet site A small tip: the column Type describes special features regarding the performance of the test, such as evaluation with the aid of color charts or comparators, the use of additional reagents, etc. 6

7 Index A Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page A Absorbance A Spectroquant PM 88 Acid Capacity Cell Test to ph mmol/l Spectroquant CT 88 (total alkalinity) mg/l CaCO 3 Alkalinity (total) see also Acid capacity to ph 4.3 Alkalinity Test mmol/l Aquamerck TP 42 Aluminium Cell Test mg/l Al Spectroquant CT 88 Aluminium Test mg/l Al Spectroquant RT 88 Aluminium Test mg/l Al Aquaquant TS 42 Aluminium Test mg/l Al Microquant DC 42 Aluminium Test mg/l Al Merckoquant R 32 Ammonium Cell Test mg/l NH 4 -N Spectroquant CT mg/l NH 4 Ammonium Test mg/l NH 4 -N Spectroquant RT mg/l NH Ammonium Test mg/l NH Aquaquant TL 42 Ammonium Test mg/l NH Aquaquant TL 42 Ammonium Test mg/l NH Aquamerck SC 42 Ammonium Test mg/l NH Reflectoquant R 62 Ammonium Test mg/l NH Aquaquant TS 42 Ammonium Test mg/l NH Microquant DC 42 Ammonium Cell Test mg/l NH 4 -N Spectroquant CT mg/l NH 4 Ammonium Test in fresh- and seawater mg/l NH Aquamerck CC 42 Ammonium Test mg/l NH Aquamerck CC 42 Ammonium Cell Test mg/l NH 4 -N Spectroquant CT mg/l NH 4 Ammonium Test mg/l NH 4 -N Spectroquant RT mg/l NH 4 Ammonium Cell Test mg/l NH 4 -N Spectroquant CT mg/l NH 4 AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 7

8 Index A-C Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page A Ammonium Test mg/l NH Reflectoquant R 62 Ammonium Test mg/l NH Merckoquant R 32 Ammonium Test mg/l NH Reflectoquant R 62 Antimony mg/l Sb Spectroquant AP 88 AOX Cell Test mg/l AOX Spectroquant CT 88 Arsenic Test mg/l As Spectroquant RT 88 Arsenic Test mg/l As Merckoquant R 32 Arsenic Test mg/l As Merckoquant R 32 Ascorbic Acid Test mg/l Ascorbic Acid Reflectoquant T 62 Ascorbic Acid Test RQeasy mg/l Ascorbic Acid Reflectoquant T 62 Ascorbic Acid Test mg/l Ascorbic Acid Merckoquant T 32 B BOD Cell Test mg/l BOD Spectroquant CT 90 Boron Test mg/l B Spectroquant RT 90 Boron Cell Test mg/l B Spectroquant CT 90 Bromate mg/l BrO 3 Spectroquant AP 90 Bromine Test mg/l Br Spectroquant RT 90 C Cadmium Test mg/l Cd Spectroquant RT 90 Cadmium Cell Test mg/l Cd Spectroquant CT 90 Calcium Test mg/l Ca Spectroquant RT 90 Calcium Test mg/l Ca Aquamerck TP 42 Calcium Test mg/l Ca Reflectoquant R 62 Calcium Test mg/l Ca Reflectoquant T 62 Calcium Test mg/l Ca Spectroquant RT mg/l CaO mg/l CaCO mg/l Ca mg/l CaO mg/l CaCO 3 Calcium Test mg/l Ca Merckoquant R 32 Calcium Cell Test mg/l Ca Spectroquant CT mg/l CaO mg/l CaCO 3 Carbohydrazide see Oxygen Scavengers Test Carbonate Hardness Test / e Aquamerck TP 42 Acid capacity to ph 4.3 ( SBV, ANC) (ANC mmol/l) Carbonate Hardness Test / 1 drop corresponds to 1.25 e Aquamerck TD 42 Acid capacity to ph 4.3 ( SBV, ANC) Carbonate Hardness Test in fresh- and seawater 1 drop corresponds to 1.25 e Aquamerck TD 42 8

9 Index C Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page C Carbonate Hardness Test 5-30 e Merckoquant T 32 Chloride Test mg/l Cl Aquamerck TP 42 Chloride Test mg/l Cl Spectroquant RT Chloride Test mg/l Cl Microquant DC 42 Chloride Cell Test mg/l Cl Spectroquant CT 90 Chloride Test mg/l Cl Aquaquant TS 42 Chloride Test 1 drop corresponds to 25 mg/l Cl Aquamerck TD 42 Chloride Test mg/l Cl Merckoquant T 32 Chlorine Test (free chlorine) mg/l Cl Aquaquant TL 42 Chlorine Test (free chlorine) mg/l Cl Spectroquant RT 90 USEPA approved Chlorine Cell Test (free chlorine) mg/l Cl Spectroquant CT 90 USEPA approved Chlorine Test (free chlorine) mg/l Cl Aquamerck CC 42 in freshwater and seawater Chlorine Test (free chlorine) (liquid) mg/l Cl Microquant DC 42 Chlorine Test (free chlorine) mg/l Cl Microquant DC 44 Chlorine Test (free chlorine) mg/l Cl Reflectoquant R 62 Chlorine Test (free chlorine) mg/l Cl Merckoquant T 32 Chlorine Test (free chlorine) mg/l Cl Merckoquant T 32 Chlorine Test (total chlorine) mg/l Cl Spectroquant RT 90 USEPA approved Chlorine Test (free and total chlorine) mg/l Cl (100 each) Spectroquant RT 92 USEPA approved Chlorine Cell Test mg/l Cl (100 each) Spectroquant CT 92 (free and total chlorine) USEPA approved AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 9

10 Index C Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page C Chlorine Test (liquid) mg/l Cl (400 each) Microquant DC 42 (free and total chlorine) Chlorine Test mg/l Cl (400 each) Microquant DC 44 (free and total chlorine) Chlorine Reagent Cl 2-1 (liquid) mg/l Cl Spectroquant RT 92 Chlorine Reagent Cl 2-2 (liquid) mg/l Cl Spectroquant RT 92 Chlorine Reagent Cl 2-3 (liquid) mg/l Cl Spectroquant RT 92 Chlorine- and ph Test mg/l Cl (chlorine) Aquamerck SC 44 (free chlorine) ph (ph) Chlorine- and ph Test mg/l Cl (chlorine) Aquamerck CV 44 (free and total chlorine) ph (ph) Chlorine Dioxide Test mg/l ClO Aquaquant TL 44 Chlorine Dioxide Test mg/l ClO Spectroquant RT 92 Chlorine Dioxide Test mg/l ClO Microquant DC 44 Chromate Test mg/l CrO Aquaquant TL 44 Chromate Test mg/l Cr Spectroquant RT 92 for the determination of chromium (VI) mg/l CrO 4 Chromate Cell Test mg/l Cr Spectroquant CT 92 for the determination of chromium (VI) mg/l CrO 4 and chromium (total) USEPA approved Chromate Test mg/l CrO Aquaquant TS 44 Chromate Test mg/l CrO Microquant DC 44 Chromate Test mg/l CrO Merckoquant R 32 Chromium in electroplating baths g/l CrO 3 Spectroquant AP 92 Cobalt Test mg/l Co Merckoquant T 32 COD Cell Test USEPA approved mg/l COD Spectroquant CT 92 COD Cell Test mg/l COD Spectroquant CT 92 COD Cell Test USEPA approved mg/l COD Spectroquant CT 92 COD Cell Test USEPA approved mg/l COD Spectroquant CT 92 COD Cell Test USEPA approved mg/l COD Spectroquant CT 94 COD Cell Test USEPA approved mg/l COD Spectroquant CT 94 COD Cell Test USEPA approved mg/l COD Spectroquant CT 94 COD Cell Test USEPA approved mg/l COD Spectroquant CT 94 COD Cell Test mg/l COD Spectroquant CT 94 COD Cell Test (Hg free) mg/l COD Spectroquant CT 94 COD Cell Test (Hg free) mg/l COD Spectroquant CT 94 Color, true and apparent Pt/Co or Cu Spectroquant PM 94 Color measurement m -1 Spectroquant PM 94 Color Test Hz no limit Aquaquant TL 44 Compact Laboratory for aquaristics Aquamerck 53 Compact Laboratory for soil analysis Agroquant 55 10

11 Index C-F Visual and instrumental test kits Parameter Measuring range No. of test Ord. No. System Type Page C Compact Laboratory for water testing Aquamerck 54 Congo red paper ph < 3 blue-violet / > 5 red-orange 3 x 4.8 m ph test paper 24 Copper Test mg/l Cu Spectroquant RT 94 Copper Test mg/l Cu Aquaquant TL 44 Copper Cell Test mg/l Cu Spectroquant CT 94 Copper Test in freshwater and seawater mg/l Cu Aquamerck CC 44 Copper Test mg/l Cu Aquaquant TS 44 Copper Test mg/l Cu Microquant DC 44 Copper Test mg/l Cu Merckoquant T 32 Copper in electroplating baths g/l Cu Spectroquant AP 94 Cyanide Test mg/l CN Aquaquant TL 44 Cyanide Test for the determination of free and mg/l CN Spectroquant RT 94 readily liberated cyanide Cyanide Cell Test for the determination of free mg/l CN Spectroquant CT 94 and readily liberated cyanide USEPA approved Cyanide Test mg/l CN Aquaquant TS 44 Cyanide Test mg/l CN Microquant DC 44 Cyanide Test 1-30 mg/l CN Merckoquant R 32 Cyanuric acid Test mg/l Cyanuric acid Spectroquant RT 94 D DEHA (Diethylhydroxylamine) see Oxygen Scavengers Test Detergents see Surfactants E Ethanol see Alcohol F Fluoride Cell Test mg/l F Spectroquant CT mg/l F Fluoride Cell Test mg/l F Spectroquant CT mg/l F Fluoride Test mg/l F Spectroquant RT Fluoride Test mg/l F Aquamerck CC 44 Formaldehyde Test mg/l HCHO Spectroquant RT 96 Formaldehyde Test mg/l HCHO Aquamerck SC 44 Formaldehyde Cell Test mg/l HCHO Spectroquant CT 96 AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 11

12 Index F-L Visual and instrumental test kits Parameter Measuring range No. of tests Ord.No. System Type Page F Formaldehyde Test mg/l HCHO Reflectoquant R 62 Formaldehyde Test mg/l HCHO Merckoquant R 32 Free Sulfurous Acid Test mg/l SO Reflectoquant R 62 Fritest Fritest 50 G Glucose Test mg/l Glucose Reflectoquant T 62 Glucose Test mg/l Glucose Merckoquant T 32 Glutardialdehyde Test 0-2.5% Merckoquant T 32 Gold Test mg/l Au Spectroquant RT 96 H Hardness see Residual Hardness or Total Hardness Hazen Color Number (Pt/Co, APHA, Hazen) Pt/Co or Cu Spectroquant PM 96 Hydrazine Test mg/l N 2 H Spectroquant RT 96 Hydrazine Test mg/l N 2 H Aquamerck CV 44 Hydrogen Peroxide see also Peroxide Hydrogen Peroxide Test mg/l H 2 O Spectroquant RT 96 Hydrogen Peroxide Cell Test mg/l H 2 O Spectroquant CT mg/l H 2 O 2 Hydrogen sulfide see Sulfide Hydroquinone see Oxygen Scavengers Test Hydroxymethylfurfural Test mg/l HMF Reflectoquant T 62 I Iodine Color Number IFZ Spectroquant PM 96 Iodine Test mg/l I Spectroquant RT 96 Iron Test mg/l Fe Spectroquant RT Iron Test mg/l Fe Aquaquant TL 46 Iron Test mg/l Fe Spectroquant RT 96 Iron Test in freshwater and seawater mg/l Fe Aquamerck CC 46 Iron Cell Test mg/l Fe Spectroquant CT 96 Iron Test mg/l Fe Microquant DC 46 Iron Test mg/l Fe Aquamerck CV 46 Iron Test mg/l Fe Aquaquant TS 46 Iron Test mg/l Fe Aquaquant TS 46 Iron Test mg/l Fe(II) Reflectoquant T 62 Iron Cell Test mg/l Fe Spectroquant CT 96 Iron Test mg/l Fe(II) Merckoquant T 32 Iron Test mg/l Fe(II) Reflectoquant T 62 Isoascorbic acid (Erythorbic acid) see Oxygen Scavengers Test L Lactic Acid Test mg/l Lactic acid Reflectoquant T 62 Lead Test mg/l Pb Spectroquant RT 96 Lead Cell Test mg/l Pb Spectroquant CT 96 Lead Test mg/l Pb Merckoquant R 32 Lead(II) acetat paper Sulfide from 10 mg/l 3 x 4.8 m Reagent paper 34 12

13 Index L-N Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page L Litmus paper, blue ph < 7 red / > 7 blue 3 x 4.8 m ph test paper 24 Litmus paper, neutral ph < 7 red / > 7 blue 3 x 4.8 m ph test paper 24 Litmus paper, red ph < 7 red / > 7 blue 3 x 4.8 m ph test paper 24 M Magnesium Cell Test mg/l Mg Spectroquant CT 96 Magnesium Test mg/l Mg Reflectoquant T 62 Magnesium Test mg/l Mg Aquamerck CC 46 Malic Acid Test mg/l Malic acid Reflectoquant T 62 Manganese Test mg/l Mn Spectroquant RT 96 Manganese Test mg/l Mn Spectroquant RT Manganese Test mg/l Mn Aquaquant TL 46 Manganese Cell Test mg/l Mn Spectroquant CT 96 Manganese Test mg/l Mn Microquant DC 46 Manganese Test mg/l Mn Merckoquant R 32 Mercury Hg Spectroquant AP 96 Methylethylketoxime (2-Butanoneoxime) see Oxygen Scavengers Test Molybdenum Cell Test mg/l Mo Spectroquant CT mg/l MoO mg/l Na 2 MoO 4 Molybdenum Test mg/l Mo Spectroquant RT mg/l MoO mg/l Na 2 MoO 4 Molybdenum Test mg/l Mo Merckoquant R 32 Monochloramine Test mg/l Cl Spectroquant RT mg/l NH 2 Cl mg/l NH 2 Cl-N N Nickel Test mg/l Ni Aquaquant TL 46 Nickel Test mg/l Ni Spectroquant RT 98 Nickel Cell Test mg/l Ni Spectroquant CT 98 Nickel Test mg/l Ni Microquant DC 46 Nickel Test mg/l Ni Merckoquant T 32 Nickel in electroplating baths g/l Ni Spectroquant AP 98 AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 13

14 Index N Visual and instrumental test kits Parameter Measuring range No. of tests Ord.No. System Type Page N Nitrate Test mg/l NO 3 -N Spectroquant RT mg/l NO Nitrate Test mg/l NO 3 -N Spectroquant RT mg/l NO 3 Nitrate Cell Test mg/l NO 3 -N Spectroquant CT mg/l NO 3 Nitrate Cell Test mg/l NO 3 -N Spectroquant CT mg/l NO 3 Nitrate Test 3-90 mg/l NO Reflectoquant T 62 Nitrate Cell Test mg/l NO 3 -N Spectroquant CT mg/l NO 3 Nitrate Test 5-90 mg/l NO Microquant DC 46 Nitrate Test mg/l NO Reflectoquant T 62 Nitrate Test RQeasy mg/l NO Reflectoquant T 62 Nitrate Test in freshwater mg/l NO Aquamerck CC 46 Nitrate Test mg/l NO Aquamerck SC 46 Nitrate Test mg/l NO Merckoquant T Nitrate Test mg/l NO Merckoquant IS 32 Nitrate Cell Test mg/l NO 3 -N Spectroquant CT mg/l NO 3 Nitrate Cell Test in seawater mg/l NO 3 -N Spectroquant CT mg/l NO 3 Nitrate Test in seawater mg/l NO 3 -N Spectroquant RT mg/l NO 3 Nitrite Test mg/l NO Aquaquant TL 46 Nitrite Test mg/l NO 2 -N Spectroquant RT mg/l NO Nitrite Test mg/l NO Aquamerck SC 46 Nitrite Cell Test mg/l NO 2 -N Spectroquant CT mg/l NO 2 Nitrite Test in freshwater and seawater mg/l NO Aquamerck CC 46 Nitrite Test mg/l NO Aquaquant TS 46 Nitrite Test mg/l NO Microquant DC 46 Nitrite Test mg/l NO Merckoquant T 32 Nitrite Test mg/l NO Reflectoquant T 62 Nitrite Test 2-80 mg/l NO Merckoquant T Nitrite Cell Test mg/l NO 2 -N Spectroquant CT mg/l NO 2 Nitrite Test g/l NO Reflectoquant T 62 Nitrite Test g/l NO Merckoquant T 32 Nitrogen (total) Cell Test mg/l N Spectroquant CT 98 Nitrogen (total) Cell Test mg/l N Spectroquant CT 98 Nitrogen (total) Cell Test mg/l N Spectroquant CT 98 14

15 Index O-P Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page O Organic Carbon, Total see TOC Oxifrit Test Oxifrit Test 50 Oxygen Test mg/l O Aquamerck TP 46 Oxygen Test 1 drop corresponds to 0.5 mg/l O Aquamerck TD 46 Oxygen Cell Test mg/l O Spectroquant CT 100 Oxygen Test in freshwater and seawater 1-12 mg/l O Aquamerck CC 46 Oxygen demand, biochemical see BOD Oxygen demand, chemical see COD Oxygen Scavengers Test mg/l DEHA Spectroquant RT mg/l Carbohy mg/l Hydro mg/l ISA mg/l MEKO Ozone Test mg/l O Aquaquant TL 48 Ozone Test mg/l O Spectroquant RT Ozone Test mg/l O Microquant DC 48 P Palladium mg/l Pd Spectroquant AP 100 Peracetic Acid Test mg/l Peracetic acid Reflectoquant T 62 Peracetic Acid Test 5-50 mg/l Peracetic acid Merckoquant T 32 Peracetic Acid Test mg/l Peracetic acid Reflectoquant T 62 Peracetic Acid Test mg/l Peracetic acid Merckoquant T 32 Peracetic Acid Test mg/l Peracetic acid Merckoquant T 32 Peroxidase Test yes/no result Merckoquant R 32 Peroxide see also Hydrogen peroxide Peroxide Test mg/l H 2 O Reflectoquant T 62 Peroxide Test mg/l H 2 O Merckoquant T Peroxide Test mg/l H 2 O Merckoquant T 34 Peroxide Test mg/l H 2 O Reflectoquant T 62 Peroxide Test mg/l H 2 O Merckoquant T 34 ph indicator papers see separate list of ph indicator papers 3 x 4.8 m ph test paper 24 ph indicator strips see separate list of ph indicator strips 100 ph test strips 25 ph indicator liquid ph ml Aquamerck CC 48 ph Test ph Reflectoquant T 62 ph Test ph Reflectoquant T 62 ph Universal indicator liquid ph ml Aquamerck CC 48 1 l AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 15

16 Index P Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page P ph Test ph Aquamerck CV 48 ph Test ph Aquamerck SC 48 ph Test in freshwater and seawater ph Aquamerck CC 48 ph Cell Test ph Spectroquant CT 100 ph Test in swimming pool ph Aquamerck CC 48 ph Test for Cooling Lubricants ph Reflectoquant T 62 ph indicator liquid ph ml Aquamerck CC 48 Phenol Test mg/l Phenol Spectroquant RT mg/l Phenol Phenol Cell Test mg/l Phenol Spectroquant CT 100 Phenolphthalein paper ph < 8.5 colorless / > 8.5 red 3 x 4.8 m ph test paper 24 Phosphatase, Alkaline see Alkaline Phosphatase Phosphate Test mg/l PO 4 -P Spectroquant RT 100 (ortho-phosphate) mg/l PO mg/l P 2 O 5 Phosphate Test mg/l PO Aquaquant TL 48 Phosphate Test RQflex plus mg/l PO Reflectoquant RT 62 Phosphate Cell Test mg/l PO 4 -P Spectroquant CT 100 (ortho-phosphate) mg/l PO mg/l P 2 O 5 Phosphate Cell Test mg/l PO 4 -P Spectroquant CT 100 (ortho-phosphate and total phosphorus) mg/l PO 4 USEPA approved mg/l P 2 O 5 Phosphate Test in freshwater and seawater mg/l PO Aquamerck CC 48 Phosphate Test mg/l PO Microquant DC 48 Phosphate Test mg/l PO Aquamerck CV 48 Phosphate Cell Test (ortho-phosphate) mg/l PO 4 -P mg/l PO mg/l P 2 O Spectroquant CT 100 Phosphate Cell Test mg/l PO 4 -P Spectroquant CT 100 (ortho-phosphate and total phosphorus) mg/l PO 4 USEPA approved mg/l P 2 O 5 Phosphate Cell Test mg/l PO 4- P Spectroquant CT 100 (ortho-phosphate) mg/l PO mg/l P 2 O 5 Phosphate Test mg/l PO 4 -P Spectroquant RT 100 (ortho-phosphate) mg/l PO mg/l P 2 O 5 Phosphate Test mg/l PO Aquaquant TS 48 Phosphate Test mg/l PO 4 -P Spectroquant RT 100 (ortho-phosphate) mg/l PO mg/l P 2 O 5 Phosphate Test mg/l PO Microquant DC 48 Phosphate Test mg/l PO Reflectoquant R 62 Phosphate Cell Test mg/l PO 4 -P Spectroquant CT 102 (ortho-phosphate) mg/l PO mg/l P 2 O 5 16

17 Index P-S Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page P Phosphate Cell Test (ortho-phosphate and total phosphorus) mg/l PO 4 -P mg/l PO mg/l P 2 O Spectroquant CT 102 Phosphate Test mg/l PO Merckoquant R 34 Platinum mg/l Pt Spectroquant AP 102 Platinum-Cobalt Standard Method see Color Potassium Test RQflex plus mg/l K Reflectoquant RT 62 Potassium Cell Test mg/l K Spectroquant CT 102 Potassium Cell Test mg/l K Spectroquant CT 102 Potassium Test g/l K Reflectoquant R 62 Potassium Test mg/l K Merckoquant R 34 Potassium iodide-starch paper Oxidizing agents 3 x 4.8 m Reagent paper 34 Protein-Test g/l Protein Spectroquant RT 102 Protein-Test g/l Protein Spectroquant RT 102 Q Quaternary Ammonium Compounds see also Surfactants (cationic) Quaternary Ammonium Compounds mg/l Benzalkonium chloride Merckoquant T 34 R Residual Hardness Test e Aquamerck CC mg/l CaCO 3 Residual Hardness Cell Test mg/l Ca Spectroquant CT d e f mg/l CaO mg/l CaCO 3 S SAC (Spectral absorption coefficient) m -1 Spectroquant PM 102 Silicate (Silicic Acid) Test mg/l SiO Spectroquant RT mg/l Si Silicate (Silicic Acid) Test mg/l Si Aquaquant TL mg/l SiO 2 Silicate (Silicic Acid)Test mg/l Si Microquant DC mg/l SiO 2 Silicate (Silicic Acid) Test mg/l SiO Spectroquant RT mg/l Si Silicic Acid see Silicate Silver Test mg/l Ag Spectroquant RT 102 Sodium Cell Test mg/l Na Spectroquant CT 102 in nutrient solution for fertilization Sucrose Test g/l Reflectoquant R 62 Sulfate Cell Test USEPA approved mg/l SO Spectroquant CT 102 Sulfate Test mg/l SO Spectroquant CT 102 Sulfate Test mg/l SO Microquant T 48 Sulfate Test mg/l SO Aquaquant TS 48 AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 17

18 Index S-T Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page S Sulfate Cell Test mg/l SO Spectroquant CT 102 Sulfate Cell Test USEAP approved mg/l SO Spectroquant CT 102 Sulfate Test mg/l SO Merckoquant DC 34 Sulfide Test mg/l S Aquaquant TL 50 Sulfide Test mg/l S Spectroquant RT 104 Sulfide Test mg/l S Microquant DC 50 Sulfite Test mg/l Na 2 SO Aquamerck TP 50 ( mg/l SO 3 ) Sulfite Cell Test mg/l SO Spectroquant CT mg/l SO 3 Sulfite Test mg/l SO Spectroquant RT mg/l SO 2 Sulfite Test mg/l SO Reflectoquant T 62 Sulfite Test mg/l SO Merckoquant T 34 Sulfurous Acid, free see Free Sulfurous Acid Sulfurous Acid, total see Total Sulfurous Acid Surfactants (anionic) Cell Test mg/l MBAS Spectroquant CT 104 Surfactants (cationic) Cell Test mg/l CTAB Spectroquant CT 104 Surfactants (nonionic) Cell Test mg/l Triton X Spectroquant CT 104 Suspended Solids suspended solids Spectroquant PM 104 T Tin Cell Test mg/l Sn Spectroquant CT 104 Tin Test mg/l Sn Merckoquant R 34 TOC Cell Test mg/l TOC Spectroquant CT 104 TOC Cell Test mg/l TOC Spectroquant CT 104 Total Acidity Test ph g/l (as Tartaric acid) Reflectoquant R 62 Total Alkalinity see Acid capacity to ph 4.3 or Alkalinity Total Hardness Test e Aquamerck TP 50 (1-100 mg/l CaCO 3 ) Total Hardness Test d Reflectoquant T 62 Total Hardness Test e Aquamerck TP 50 ( mmol/l) 18

19 Index T-Z Visual and instrumental test kits Parameter Measuring range No. of tests Ord. No. System Type Page T Total Hardness Cell Test mg/l Ca Spectroquant CT d e f mg/l CaO mg/l CaCO 3 Total Hardness Test in freshwater 1 drop corresponds to 1.25 e Aquamerck TD 50 Total Hardness Test 1 drop corresponds to 1.25 e Aquamerck TD 50 Total Hardness Test 1 drop corresponds to 20 mg/l CaCO Aquamerck TD 50 Total Hardness Test 4-26 e Merckoquant T 34 Total Hardness Test 4-26 e Merckoquant IS 34 Total Hardness Test 4-26 e Merckoquant S 34 Total Hardness Test 6-31 e Merckoquant T 34 Total Hardness Test 6-31 e Merckoquant IS 34 Total Hardness Test < > 2.5 mmol/l CaCO Merckoquant T 34 Total Nitrogen see Nitrogen (total) Total Sugar Test (glucose and fructose) mg/l total sugar Reflectoquant R 62 Transmission % T Spectroquant PM 104 Turbidity see chapter Turbiquant Turbiquant 134 Turbidity FAU Spectroquant PM 104 U Urea Test mg/l Urea Microquant DC 50 Urea Test in Milk Application mg/l NH Reflectoquant R 62 V Volatile Organic Acids Cell Test mg/l acetic acid Spectroquant CT 104 W Water Hardness see Residual Hardness or Total Hardness Z Zinc Cell Test mg/l Zn Spectroquant CT 104 Zinc Test mg/l Zn Spectroquant RT 104 Zinc Test mg/l Zn Microquant DC 50 Zinc Test mg/l Zn Aquaquant TS 50 Zinc Cell Test mg/l Zn Spectroquant CT 104 Zinc Test 4-50 mg/l Zn Merckoquant R 34 AP application DC disk comparator R incl. reagent ST indicator strips TP titration with pipette CC color card IS individually sealed RT reagent test T test strip TS tubes, short CT cell test PA indicator paper S single test strips TD titration with dropping bottle CV color-matching vessel PM physical method SC sliding comparator TL tubes, long 19

20 "Why can t my university tests be as easy as ph tests from Merck?" Cho Li X., undergraduate Research or industry? Cho isn t sure which to choose when she graduates. Either way, she s sure to find our ph test strips and papers waiting on the desk - just as she did at college. Even after forty years, there s no better way to determine ph values easily and quickly. 20

21 ph test strips and papers General information 22 ph test strips and papers 24 21

22 ph test strips and papers ph determination made easy To measure the ph swiftly and without using instruments, simply use Merck s all-purpose ph indicator papers and strips. The ph tests are suited for all media in environmental analysis and in industrial in-process controls. The visual Aquamerck test kits are predestined for all applications in which the use of ph indicator solutions is required. The broad ph test range offers you an optimal solution for each application area. 22

23 The advantages of Merck s ph tests Rapid on-the-spot ph determination More precise evaluation thanks to differentiated color graduations Expressive results for environmental analysis and in-process controls Calibration and checking of the products by certified buffer solutions ph indicator papers The traditional option: Our ph indicator papers consist of high-quality filter papers that are impregnated with indicator or mixed-indicator solutions, then dried and cut to size. We generally supply them in the roll format. This has the advantage that the tests can be better protected against external effects, such as e.g. moisture, light, and ambient gases, and can thus be stored for a longer time. ph indicator strips non-bleeding The universal option: Our ph indicator strips contain special indicator dyes that are covalently bound to the reagent papers. This special method of manufacture gives the ph indicator strips decisive advantages over the indicator papers: since the indicator does not bleed, the strips can be left in place in the measurement medium for an indefinite time without contaminating the medium. Shelf-life and storage Our indicator papers and strips can be used for 3 to 5 years when stored at temperatures of 10 to 25 C, protected from light in a dry laboratory atmosphere. To ensure optimal protection of the tests, the package should be closed immediately after removal of each strip or paper. Package sizes We standardly pack our indicator papers in lengths of 4.8 meters in a roll dispenser. Our indicator test strips are packed in boxes containing 100 strips each. On request, from a defined order size upwards, the customer can also receive singly sealed test strips and indicator papers in other formats. Quality assurance Merck checks and calibrates its tests using certified buffer solutions. These buffer solutions can be traced back directly to primary reference materials originating from NIST and PTB. This enables us to maintain a consistently high quality of our ph tests. 23

24 ph test strips and papers ph indicator papers Product ph-measuring Graduation Roll length/ Ord. No. range number of strips Roll format ph-box x 4.8m ph-indicator paper Replacement rolls* x 4.8m ph-indicator paper Replacement rolls* x 4.8m ph-indicator paper Replacement rolls* x 4.8m ph-indicator paper Universal indicator x 4.8m ph-indicator paper Replacement rolls* 6 x 4.8m ph-indicator paper Universal indicator x 4.8m ph-indicator paper Replacement rolls* 6 x 4.8m ph-indicator paper Acilit x 4.8m ph-indicator paper Replacement rolls* 6 x 4.8m ph-indicator paper Neutralit x 4.8m ph-indicator paper Replacement rolls* 6 x 4.8m ph-indicator paper Alkalit x 4.8m ph-indicator paper Replacement rolls* 6 x 4.8m ph-indicator paper Special indicator < x 4.8m ph-indicator paper Special indicator < >7.0 3 x 4.8m ph-indicator paper Special indicator >8.0 3 x 4.8m ph-indicator paper Special indicator < x 4.8m Litmus paper, blue Reag. Ph Eur ph < 7 red / > 7 blue - 3 x 4.8m Litmus paper, neutral ph < 7 red / > 7 blue - 3 x 4.8m Litmus paper, red Reag. Ph Eur ph < 7 red / > 7 blue - 3 x 4.8m Congo red paper Reag. Ph Eur ph < 3 blue-violet / - 3 x 4.8m > 5 red-orange Phenolphthalein paper ph < 8.5 colorless / - 3 x 4.8m > 8.5 red Booklet format ph-indicator paper Universal indicator x On request we are ready to supply ph-indicator paper as special customer orders in other presentations or dimensions, such as DIN A4 and larger. * Replacement roll without color scale 24

25 ph indicator strips (non-bleeding) Product ph-mesasuring Graduation Number of Ord. No. range test strips ph-indicator strips Universal indicator ph-indicator strips Acilit ph-indicator strips Neutralit ph-indicator strips Alkalit ph-indicator strips ph-indicator strips Special indicator ph-indicator strips Special indicator ph-indicator strips Special indicator ph-indicator strips Special indicator ph-indicator strips Special indicator For professional use ph-indicator strips Special indicator for ph-measurements in turbid solutions (suspensions) ph-indicator strips Special indicator for ph-measurements in meat ph-indicator strips, CE-certified for in vitro diagnostics for semi-quantitative determination of ph in human urine ph-indicator strips, singly sealed ph-indicator strips, singly sealed Individually sealed ph-indicator strips are available on request, also for other ph-ranges. ph indicator strips Singly sealed ph indicator strips can be included or stuck in books, magazines, or brochures. These ph indicator strips are also used for product promotion and product differentiation purposes by adding them to a specific product. 25

26 My customers trust me for fast deliveries. And Merckoquant test strips for fast results. Miguel C., farmer Freshness won t wait. So when Miguel brings his oranges to the factory, his customers start testing the ascorbic acid content right away. Thanks to Merckoquant indicator strips, they get reliable results in minutes. And can start making juice long before Miguel gets back home. 26

27 Test strips Merckoquant General information 28 Merckoquant test strips 32 Reagent papers 34 27

28 Test strips Merckoquant Universal, fast and simple Test strips actually constitute a genuine high-tech product: each one is a mobile lab taking up just a few square millimeters and easily fits in your pocket to be taken wherever you go. Merckoquant test strips are ideally suited for the semi quantitative determination of ions and compounds. They can be used in concentration ranges from 1 resp. 10 mg/l right up to the g/l range. As a screening tool they enable the user to run a swift check on the contents of his sample and help save many time-consuming and cost-intensive analytical procedures. Quality- and process control operations are carried out more rapidly. Thanks to the PET film and the low reagent content, the test strips are also easy to dispose of. 28

29 The advantages of the Merckoquant test strips Small and easy to handle for on-the-spot use Results provided fast and easily Broad range with over 50 tests Flexible and universal application Ideal for screening time and cost-saving Merckoquant take your pick! Merckoquant test strips yield a rapid measurement result in the semiquantitative range for over 30 parameters. In some tests the sample must be pretreated. Again; no problem all the necessary reagents are included in the pack. Some test strips are also available individually sealed. This keeps them protected against external influences, and they can be easily inserted or stuck in books, magazines, and brochures. From a given order volume up, Merckoquant test strips can also be supplied in the customer's own package design. 29

30 Merckoquant Merckoquant it s that simple! 1. Merckoquant test strips couldn t be easier to handle. 2. The reaction zones are wetted with the solution to be tested simply by dipping them briefly into the liquid sample. Any excess liquid is then shaken off or removed by drawing the test strip across the edge of the vessel. 3. After the specified reaction time of at most one minute has expired, the color of the reaction zone is compared with the color scale printed on the tube label and the corres ponding concentration is read off. Shelf-life and storage When stored cool (refrigeration is necessary in some cases) and dry, the test strips can be used at least up to the expiry date printed on the pack. The tube must be reclosed immediately after the removal of each strip to ensure the remaining test strips are protected. Package sizes Our standard package consists of 100 test strips packed in an aluminium tube. For certain parameters we also offer packs containing 25 test strips. In cases in which sample preparation is necessary, the additionally required reagents are also included in the pack. Quality assurance Merck checks and calibrates its tests and the exact comparison colors using certified standard solutions. These solutions can be traced back directly to primary reference materials originating from NIST and PTB. This enables us to achieve a consistently high quality of our Merckoquant test strips. Merckoquant Blank Test Strips (Ord. No ) The blank test strips incorporate a paperzone not impregnated with reagent. They are used to perform tests to see whether sample solutions turn the blank zone a different color. A difference in color can indicate that the intrinsic color of the sample may affect results obtained with other Merckoquant test strips. 30

31 ph and Merckoquant test strips available with your own brand name Are you interested in ph and Merckoquant test strips customized to your requirements or with a private label? To support and enhance your product promotion, the single sealed test strips or the test strips in a tube with your own label are the ideal solution. Merck provides you with three different options: Single sealed test strips Individually sealed test strips can be enclosed loose or with adhesive in books, magazines and brochures. These strips can also be used, however, for product promotion and differentiation purposes and enclosed with a specific product. Your benefits: Unique and highly innovative technology products customized to your requirements Support for your product promotion Tubes with list items or tailor-made test strips Design your own tailor-made label and increase the awareness for your business. We offer you close cooperation from the design of the color card to the final product. The test strips are also available for other measuring ranges customized to your needs. Customized innovative products You need an unlisted test strip for your individually requirements? Merck s customized innovative products are the ideal solution. We verify the feasibility and cooperate with you on developing unique, innovative products that are highly profitable. Design your own individual innovative products customized to your requirements in cooperation with Merck. For more details please see 31

32 Index A-P Merckoquant test strips Parameter Graduation No. of tests Ord. No. Method Type A Aluminium Test mg/l Al Aurin tricarboxylic acid R Ammonium Test mg/l NH Neßler R Arsenic Test mg/l As modified Gutzeit test R Arsenic Test mg/l As modified Gutzeit test R mg/l As Ascorbic Acid Test Phosphormolybdenum blue mg/l ascorbic acid B Blank strip C Calcium Test mg/l Ca Glyoxal bishydroxyanil R Carbonate Hardness Test e Mixed indicator Chloride Test mg/l Cl Silver chromate Chlorine Test mg/l Cl Redox reaction Chlorine Test mg/l Cl Redox reaction Chromate Test mg/l CrO Diphenylcarbazide R Cobalt Test mg/l Co Rhodanide Copper Test mg/l Cu ,2 -Cuproin Cyanide Test mg/l CN Barbituric acid derivative R F Formaldehyde Test mg/l HCHO Triazole R G Glucose Test mg/l Glucose Enzymatic reaction Glutardialdehyde Test % Redox reaction I Iron Test mg/l Fe(II) ,2 -Bipyridine L Lead Test mg/l Pb Rhodizonic acid R M Manganese Test mg/l Mn Oxidation/Redox indicator R Molybdenum Test mg/l Mo Toluene-3,4-dithiol R N Nickel Test mg/l Ni Diacetyldioxime Nitrate Test mg/l NO modified Griess reaction Nitrate Test mg/l NO modified Griess reaction Nitrate Test mg/l NO modified Griess reaction IS Nitrite Test mg/l NO Griess reaction Nitrite Test mg/l NO Griess reaction Nitrite Test mg/l NO Griess reaction Nitrite Test g/l NO Griess reaction P Peracetic Acid Test mg/l Peracetic acid Redox reaction Peracetic Acid Test Redox reaction mg/l Peracetic acid Peracetic Acid Test mg/l Peracetic acid Redox reaction Peroxidase Test yes/no result Enzymatic reaction R IS individually sealed R incl. reagent 32

33 Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Aluminium Test Ammonium Test Arsenic Test Arsenic Test Ascorbic Acid Test Blank strip Calcium Test Carbonate Hardness Test Chloride Test Chlorine Test Chlorine Test Chromate Test Cobalt Test Copper Test Cyanide Test Formaldehyde Test Glucose Test Glutardialdehyde Test Iron Test Lead Test Manganese Test Molybdenum Test Nickel Test Nitrate Test Nitrate Test Nitrate Test Nitrite Test Nitrite Test Nitrite Test Nitrite Test Peracetic Acid Test Peracetic Acid Test Peracetic Acid Test Peroxidase Test 33

34 Index P-Z Merckoquant test strips Parameter Graduation No. of tests Ord. No. Method Type P Peroxide Test mg/l H 2 O Enzymatic reaction Peroxide Test mg/l H 2 O Enzymatic reaction Peroxide Test mg/l H 2 O Enzymatic reaction Peroxide Test mg/l H 2 O Enzymatic reaction Phosphate Test mg/l PO Phosphormolybdenum blue R Potassium Test mg/l K Dipicrylamine R Q Quaternary Ammonium Indicator Compounds 500 mg/l Benzalkonium chloride S Sulfate Test mg/l SO Ba-thorin complex Sulfite Test mg/l SO Nitroprusside/ Zn-hexacyanoferrate T Tin Test mg/l Sn Toluene-3,4-dithiol R Total Hardness Test e EDTA Total Hardness Test e EDTA IS Total Hardness Test e EDTA IS Total Hardness Test e EDTA Total Hardness Test e EDTA IS Total Hardness Test < 1.5; ; > 2.5 mmol/l CaCO EDTA Z Zinc Test mg/l Zn Dithizone R IS individually sealed R incl. reagent Reagent papers Lead acetate paper 3 rolls each with 4.8 meters Ord. No Lead acetate paper is used for the determination of sulfide and hydrogen sulfide Potassium iodide-starch paper, Reag. Ph Eur 3 rolls each with 4.8 meters Ord. No Potassium iodide paper is used for the determination of oxidizing agents 34

35 Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Peroxide Test Peroxide Test Peroxide Test Peroxide Test Phosphate Test Potassium Test Quaternary Ammonium Compounds Sulfate Test Sulfite Test Tin Test Total Hardness Test Total Hardness Test Total Hardness Test Total Hardness Test Total Hardness Test Total Hardness Test Zinc Test Merckoquant Sample vessels for Aquamerck and Merckoquant tests are ideally suited for the use of the Merckoquant tests with reagent. For more details please see page

36 "My protective clothing is off-the-peg. But my Microquant test assays are always made to measure." Siam L., cleaning technician When Siam checks for residue after purifying water at the bottling plant, he uses Microquant test assays. We tailor them to his extract thresholds, so he gets precise results every time. And because they work right there on site, there s no waiting for lab samples either. 36

37 Colorimetric and titrimetric test kits Aquamerck Microquant Aquaquant General information 38 Product list 42 Compact laboratories 52 37

38 Colorimetric and titrimetric test kits Aquamerck Microquant Aquaquant Variety and ease of handling Convince yourself of just how easy it is to handle our visual rapid tests. These are simple to work with, even for users without in-depth chemical skills. Each pack contains all the accessories and reagents necessary to perform the test. The reagent bottles have been designed in such a way that the liquid reagents can be easily metered drop by drop. The solid reagents can be measured in using the metering spoons integrated into the screw caps. A unique feature of these tests are the high-quality and brilliant color cards, which decisively simplify the allocation of the stated values (color dots). This gives you more precise results in no time at all. Another practical feature: the corresponding refill packs. The bottles fit directly in the packs for the respective original test kits, and the number of determinations is the same as that for the original test kits. In this way you can use the color comparators over and over again reducing your costs per determination and helping to relieve the environmental burden at the same time. The Aquamerck system comprises titrimetric and colorimetric tests (evaluation using color cards or test vessels) 38

39 The advantages of the rapid tests Practical for on-the-spot use Illustrated instructions for an easy to carry out procedure Application-oriented measuring ranges Brilliant color scales and high-quality comparators deliver reliable results Low analytical costs The visual rapid tests at a glance Aquamerck Concentration range Areas of application The range comprises Medium concentration range and seawater and titration pipettes Microquant Concentration range Areas of application The range comprises Medium to high concentration range, measurements possible in turbid or colored samples Aquaquant Concentration range Areas of application The range comprises Very low to medium concentration range 39

40 Visual rapid tests Aquamerck The Aquamerck product range comprises both titrimetric as well as colorimetric tests. Their handling could not be easier. In the titration tests the sample is titrated until the color changes, with the number of drops consumed to the turning point or the scalar value read off from a pipette corresponding to the concentration of the tested parameter. In the case of the colorimetric tests a color reaction is produced by adding reagents to the sample solution. By allocating the color to a color value on a reference scale the corresponding concentration can be read off. Microquant Microquant tests evaluate the color reaction according to the transmitted light method. This makes it possible to analyze even turbid and slightly colored water samples without any further sample preparation. Thanks to the robust color comparator disk it is also possible to use these tests without problem in industrial areas and in wet environments. The ten-stage color disk of the comparator is made of lightfast and thus extremely durable slides of plastic. Almost every test contains break-proof test vessels for more safety handling. Using the refill packs that are also available, the original test kit can be used for a very long time. Aquaquant The special strength of the Aquaquant range lies in the measurement of low concentrations. With Aquaquant tests users can detect concentrations down to the ppb range. These specifically high measurement sensitivities are made possible by use of greater layer thickness and the special construction design of the Aquaquant comparator. In the comparison method the reaction color of the sample is compared with the blank sample tinted by the color scale. This makes it possible to achieve an excellent match with a concentration. The color cards are of particular importance here. The unique brilliance of the print and the fine color graduation enable precise analyses to be made, even at the lower end of the concentration range. The Aquaquant system: The comparator method convinces in its use! 40

41 Shelf-life and storage When stored at temperatures between 15 and 25 C, the visual test kits remain stable for up to three years from the date of production. Under other storage conditions the test kit should be checked prior to use by using a standard solution adjusted in the upper third of the measuring range. If test kit detects this set value, it is still capable of being used. Quality assurance We adjust our test kits and comparison colors with the aid of certified standard solutions. These can be traced back directly to primary reference materials originating from NIST und PTB. This enables us to achieve a consistently high quality standard of all our visual test kits. The Microquant system: All reagents and the color comparator disk are contained in the kit. Practical refill packs We devote our efforts not just to the actual applications themselves, but also to ensuring that we offer you economically attractive solutions. This is why refill packs are also available for many of our test kits, reducing the price for individual analyses in the process. If you have a large specific requirement, we can also supply customer-specific packs with a modified number of determinations or different color scales. 41

42 Index A-C Aquamerck Microquant Aquaquant Parameter Graduation No. of tests Ord. No. Method System Type A Alkalinity Test 0.1 mmol/l 200 at 8.5 mmol/l Acidimetric Aquamerck TP Aluminium Test Chromazurol S Aquaquant TS mg/l Al Aluminium Test Chromazurol S Microquant mg/l Al Ammonium Test Indophenol blue Aquaquant TL mg/l NH 4 Ammonium Test Neßler Aquaquant TL mg/l NH 4 Ammonium Test mg/l NH Indophenol blue Aquamerck SC Ammonium Test Indophenol blue Aquaquant TS mg/l NH 4 Ammonium Test Indophenol blue Microquant mg/l NH 4 Ammonium Test in mg/l NH Indophenol blue Aquamerck CC freshwater and seawater Ammonium Test mg/l NH Neßler Aquamerck CC C Calcium Test 2 mg/l 200 at 170 mg/l Ca Titriplex III Aquamerck TP Carbonate Hardness Test/ 0.25 e and 0.1 mmol/l 300 at 12.5 e Acidimetric Aquamerck TP Acid cap. to ph 4.3 (ANC) Carbonate Hardness Test/ 1.25 e 100 at 12.5 e Acidimetric Aquamerck Acid cap. to ph 4.3 (ANC) Carbonate Hardness Test in 1.25 e 50 at 12.5 e Acidimetric Aquamerck freshwater and seawater Chloride Test 2 mg/l Cl 200 at 170 mg/l Cl Mercury(II)-nitrate Aquamerck TP Chloride Test Mercury(II)- Microquant mg/l Cl thiocyanate Chloride Test Mercury(II)- Aquaquant TS 300 mg/l Cl thiocyanate Chloride Test 25 mg/l Cl 100 at 150 mg/l Cl Mercury(II)-nitrate Aquamerck Chlorine Test free chlorine Aquaquant TL mg/l Cl 2 Chlorine Test in mg/l Cl free chlorine TMB Aquamerck CC freshwater and seawater Chlorine Test free chlorine Microquant mg/l Cl 2 Chlorine Test free chlorine Microquant mg/l Cl total chlorine CC color card CV color-matching vessel DC disk comparator SC sliding comparator TD titration with dropping bottle TL tubes, long TS tubes, short TP titration with pipette 42

43 Ord. No. Refill pack Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Alkalinity Test Aluminium Test Aluminium Test Ammonium Test Ammonium Test Ammonium Test Ammonium Test Ammonium Test Ammonium Test in freshwater and seawater Ammonium Test Calcium Test Carbonate Hardness Test/ Acid cap. to ph 4.3 (ANC) Carbonate Hardness Test/ Acid cap. to ph 4.3 (ANC) Carbonate Hardness Test in freshwater and seawater Chloride Test Chloride Test Chloride Test Chloride Test Chlorine Test Chlorine Test in freshwater and seawater Chlorine Test Chlorine Test 43

44 Index C-H Aquamerck Microquant Aquaquant Parameter Graduation No. of tests Ord. No. Method System Type C Chlorine Test free chlorine Microquant mg/l Cl 2 Chlorine Test free chlorine Microquant mg/l Cl total chlorine Chlorine- and ph Test mg/l Cl (chlorine) Aquamerck SC (free chlorine) ph (ph) red Phenol Chlorine- and ph Test mg/l Cl (chlorine) Aquamerck CV (free and total chlorine) ph (ph) red Phenol Aquaquant TL mg/l ClO Microquant mg/l ClO 2 Chromate Test Aquaquant TL mg/l CrO 4 Chromate Test Aquaquant TS mg/l CrO 4 Chromate Test Microquant mg/l CrO 4 Color Test no limit Hazen Aquaquant TL Hz Copper Test Cuprizone Aquaquant TL mg/l Cu Copper Test in Cuprizone Aquamerck CC freshwater and seawater mg/l Cu Copper Test Cuprizone Aquaquant TS mg/l Cu Copper Test Cuprizone Microquant mg/l Cu Cyanide Test König s reaction Aquaquant TL mg/l CN Cyanide Test König s reaction Aquaquant TS mg/l CN Cyanide Test König s reaction Microquant mg/l CN F Fluoride Test mg/l F Alizarin-komplexone Aquamerck CC Formaldehyde Test Triazole derivative Aquamerck SC 1.5 mg/l HCHO H Hydrazine Test mg/l N 2 H Aquamerck CV benzaldehyde CC color card CV color-matching vessel DC disk comparator SC sliding comparator TD titration with dropping bottle TL tubes, long TS tubes, short TP titration with pipette 44

45 Ord. No. Refill pack Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Chlorine Test Chlorine Test Chlorine- and ph Test Chlorine- and ph Test Chromate Test Chromate Test Chromate Test Color Test Copper Test Copper Test in freshwater and seawater Copper Test Copper Test Cyanide Test Cyanide Test Cyanide Test Fluoride Test Formaldehyde Test necessary Hydrazine Test 45

46 Index I-O Aquamerck Microquant Aquaquant Parameter Graduation No. of tests Ord. No. Method System Type I Iron Test Triazine Aquaquant TL mg/l Fe Iron Test in Triazine Aquamerck CC freshwater and seawater mg/l Fe Iron Test Triazine Microquant mg/l Fe Iron Test ,2 -Bipyridine Aquamerck CV mg/l Fe Iron Test Triazine Aquaquant TS mg/l Fe Iron Test ,2 -Bipyridine Aquaquant TS mg/l Fe M Magnesium Test Xylidyl blue Aquamerck CC mg/l Mg Manganese Test Formaldoxime Aquaquant TL mg/l Mn Manganese Test Formaldoxime Microquant mg/l Mn N Nickel Test Aquaquant TL mg/l Ni Nickel Test Microquant 8-10 mg/l Ni Nitrate Test Nitrospectral / Microquant mg/l NO 3 sulfuric acid Nitrate Test in Sulfanilic acid / Aquamerck CC freshwater 150 mg/l NO 3 Gentisine acid Nitrate Test Sulfanilic acid / Aquamerck SC 150 mg/l NO 3 Gentisine acid Nitrite Test Griess reaction Aquaquant TL mg/l NO 2 Nitrite Test Griess reaction Aquamerck SC mg/l NO 2 Nitrite Test in mg/l NO Griess reaction Aquamerck CC freshwater and seawater Nitrite Test Griess reaction Aquaquant TS mg/l NO 2 Nitrite Test Griess reaction Microquant 6-10 mg/l NO 2 O Oxygen Test 0.1 mg/l O at 8.5 mg/l O modified Aquamerck TP Winkler method Oxygen Test 0.5 mg/l O at 8.5 mg/l O modified Aquamerck Winkler method Oxygen Test in mg/l O modified Aquamerck CC freshwater and seawater Winkler method 46 CC color card CV color-matching vessel DC disk comparator SC sliding comparator TD titration with dropping bottle TL tubes, long TS tubes, short TP titration with pipette

47 Ord. No. Refill pack Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Iron Test Iron Test in freshwater and seawater Iron Test Iron Test Iron Test Iron Test Magnesium Test Manganese Test Manganese Test Nickel Test Nickel Test Nitrate Test Nitrate Test in freshwater Nitrate Test Nitrite Test Nitrite Test Nitrite Test in freshwater and seawater Nitrite Test Nitrite Test necessary: necessary: Oxygen Test Oxygen Test Oxygen Test in freshwater and seawater 47

48 Index O-S Aquamerck Microquant Aquaquant Parameter Graduation No. of tests Ord. No. Method System Type O Ozone Test Aquaquant TL mg/l O 3 Ozone Test Microquant mg/l O 3 P ph Universal indicator, ph ml Mixed indicator Aquamerck CC liquid ph Universal indicator, ph l Mixed indicator Aquamerck CC liquid ph Indicator liquid ph ml Mixed indicator Aquamerck CC ph Indicator liquid ph ml Mixed indicator Aquamerck CC ph Test ph Mixed indicator Aquamerck SC ph Test ph Mixed indicator Aquamerck CV ph Test in ph Mixed freshwater Aquamerck CC freshwater and seawater and seawater indicator ph Test in swimming pool ph Phenol red Aquamerck CC Phosphate Test Phosphor- Aquaquant TL mg/l PO 4 molybdenum blue Phosphate Test in Phosphor- Aquamerck CC freshwater and seawater 2-3 mg/l PO 4 molybdenum blue Phosphate Test Phosphor- Microquant mg/l PO 4 molybdenum blue Phosphate Test mg/l PO Phosphor- Aquamerck CV molybdenum blue Phosphate Test Vanadium Aquaquant TS mg/l PO 4 molybdate Phosphate Test Vanadium Microquant mg/l PO 4 molybdate R Residual Hardness Test e Mixed indicator Aquamerck CC S Silicate (Silicic Acid) Test Silico- Aquaquant TL mg/l SiO 2 molybdenum blue Silicate (Silicic Acid)Test Silico- Microquant mg/l SiO 2 molybdenum blue Sulfate Test Tannic acid Microquant mg/l SO 4 Sulfate Test Tannic acid Aquaquant TS 300 mg/l SO 4 CC color card CV color-matching vessel DC disk comparator SC sliding comparator TD titration with dropping bottle TL tubes, long TS tubes, short TP titration with pipette 48

49 Ord. No. Refill pack Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Ozone Test Ozone Test ph Universal indicator, liquid ph Universal indicator, liquid ph Indicator liquid ph Indicator liquid ph Test ph Test ph Test in freshwater and seawater ph Test in swimming pool Phosphate Test Phosphate Test in freshwater and seawater Phosphate Test Phosphate Test Phosphate Test Phosphate Test Residual Hardness Test Silicate (Silicic Acid) Test Silicate (Silicic Acid)Test Sulfate Test Sulfate Test 49

50 Index S-Z Aquamerck Microquant Aquaquant Parameter Graduation No. of tests Ord. No. Method System Type S Sulfide Test Aquaquant TL mg/l S phenylendiamine Sulfide Test Microquant mg/l S phenylendiamine Sulfite Test 0.5 mg/l Na 2 SO 3 (0.3 mg/l SO 3 ) Iodate / Starch Aquamerck TP at 40 mg/l Na 2 SO 3 T Total Hardness Test 0.13 e and 1 mg/l CaCO at 3.8 e Titriplex III Aquamerck TP Total Hardness Test 0.25 e and 0.1 mmol/l 300 at 12.5 e Titriplex III Aquamerck TP Total Hardness Test in 1.25 e 50 at 12.5 e Titriplex III Aquamerck freshwater Total Hardness Test 1.25 e 100 at 12.5 e Titriplex III Aquamerck Total Hardness Test 20 mg/l CaCO at 200 mg/l Titriplex III Aquamerck U Urea Test Indophenol blue Microquant 8 mg/l (NH 2 ) 2 CO Z Zinc Test Thiocyanate / Microquant mg/l Zn Brilliant green Zinc Test Thiocyanate / Aquaquant TS mg/l Zn Brilliant green CC color card CV color-matching vessel DC disk comparator SC sliding comparator TD titration with dropping bottle TL tubes, long TS tubes, short TP titration with pipette Parameter Graduation No. of test Ord. No. Method Fritest Perfect still good Colorimetric, acc. to the change of fat advisable alkali color count principle deep-frying fat gone off Fritest Colorimetric, acc. to the refill pack alkali color count principle Oxifrit Test fresh deep-frying fat Colorimetric, acc. to the change of fat advisable principle of determination of deep-frying fat gone off oxidized fatty acids (OFAs) Oxifrit Test Colorimetric, acc. to the refill pack principle of determination of oxidized fatty acids (OFAs) 50

51 Ord. No. Refill pack Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Sulfide Test Sulfide Test Sulfite Test Total Hardness Test Total Hardness Test Total Hardness Test in freshwater Total Hardness Test Total Hardness Test Urea Test Zinc Test Zinc Test Oxifrit Test for evaluating the degree of deterioration of fats used in deep-frying in kitchens and bakeries. Accessories for Aquamerck, Microquant, Aquaquant and Merckoquant Product Ord. No. Flat-bottomed long tubes inclusive screw caps for Aquaquant Tests (1 pack = 12 pcs) Flat-bottomed tubes inclusive screw caps for Aquamerck Test (1 pack = 12 pcs) Flat-bottomed tubes inclusive screw caps for Microquant Tests (1 pack = 12 pcs) Test vessels with 5 ml und 10 ml graduations, for Aquamerck and Merckoquant Tests (1 pack = 30 pcs) 51

52 Compact laboratories Aquamerck Agroquant On site with the lab No matter whether you wish to check a specific concentration range for a given parameter on the spot or if you just want to check a limit the Aquamerck compact laboratory contains all the reagents and accessories necessary for the application in question. The robust carry-case provides protection for the contents, and the opened cover can be used as an onthe-spot workbench. Economical refill packs are available for each parameter for the compact laboratories, too. These reagent bottles can be placed in the case directly and reduce the costs for the later determinations. 52

53 The advantages of the compact laboratories User-oriented compilation of the reagents AIl reagents in a minimum space No further instruments or accessories necessary Safely packaged Easy to handle and robust On site with the lab The compact laboratories at a glance Aquamerck compact laboratory for aquaristics Ord. No Compact laboratory for the determination of ph, carbonate hardness, total hardness, ammonium/ammonia, nitrite and nitrate in freshwater and seawater. This parameter combination can be used to check and monitor the most important quality-relevant water constituents, in freshwater and in some cases also the seawater of an aquarium. With the help of available literature data, these results can be used to control the parameter concentrations that must be observed for the fish stock. An appropriate range of separate tests are also available for other parameters that must also be monitored. Scope of delivery Parameter Measuring range No. of tests Ord. No. refill pack Aquamerck Ammonium Test mg/l NH in freshwater and seawater Aquamerck Carbonate Hardness Test 1.25 e 50 at 12.5 e Aquamerck Total Hardness Test in freshwater 1.25 e 50 at 12.5 e Aquamerck Nitrate Test in freshwater mg/l NO Aquamerck Nitrite Test mg/l NO in freshwater and seawater Aquamerck ph Test ph in freshwater and seawater Test vessels with 5 ml and 10 ml graduations 1 pack = 30 pcs for Aquamerck and Merckoquant Tests 53

54 Compact laboratories Aquamerck Agroquant Aquamerck compact laboratory for water testing Ord. No Compact laboratory for the determination of ph, ammonium, biological oxygen and nitrite, phosphate and oxygen. The tests of this compact laboratory can be used to rapidly check all major parameters of standing or flowing surface water and to assess the current water quality. The compact laboratory is optimally suited for use in school lessons as well as for professional applications e.g. by water monitors. Scope of delivery Parameter Measuring range No. of tests Ord. No. refill pack Aquamerck mg/l NH Ammonium Test Aquamerck e 300 at 12.5 e Carbonate Hardness Test/ Acid cap. to ph 4.3 (ANC) ANC: mmol/l Aquamerck 0.25 e and 0.1 mmol/l 300 at 12.5 e Total Hardness Test Aquamerck mg/l NO Nitrate Test Aquamerck mg/l NO Nitrite Test Aquamerck ph ph Test Aquamerck mg/l PO Phosphate Test in freshwater and seawater Aquamerck 0.1 mg/l O at 8.5 mg/l O Oxygen Test Flat-bottomed tubes inclusive 1 pack = 12 pcs screw caps for Aquamerck Tests Thermometer Aquamerck Oxygen Reaction bottle 1 bottle Test vessels with 5 ml and 10 ml 1 pack = 30 pcs graduation for Aquamerck and Merckoquant Tests 54

55 Agroquant soil laboratory 3 x N and ph Ord. No Compact laboratory with test strips, reagents, balance, timer and accessories for the determination of nitrate, nitrite, ammonium nitrogen and ph in soils, water, plant, animal fodder, compost, solid and liquid manure. Scope of delivery Parameter Measuring range No. of tests Ord. No. refill pack Merckoquant Nitrate Test mg/l NO ph indicator strips Universal indicator ph ph indicator strips Special indicator ph Aquamerck Ammonium Test mg/l NH Merckoquant Ammonium Test mg/l NH Agroquant Extraction Reagent Potassium chloride for analysis Sorry, we discontinued the Aquamerck Compact Laboratory for Offset Printing. But the individual components are still separately available. Please ask your local distributor for more information. 55

56 "Mum says my milk is forty-five with vitamins. Or something like that. Hannes (5), critical consumer Kids like Hannes aren t supposed to worry about fortification levels in nutrition. That s a job for grown-ups! Actually, though, making sure products contain the right amounts of healthy ingredients is child s play with Reflectoquant kits. No wonder they go down so well with manufacturers. 56

57 Reflectometry Reflectoquant General information 58 RQflex RQflex 10 plus 61 Test kits 62 57

58 Reflectometric Measurements Reflectoquant Easy in-process monitoring The simplest possible handling combined with the precision of instrumental analysis: the Reflectoquant system is in a class of its own. Monitor the raw materials you use in all stages of your production processes and thus assure a consistently high quality. The system consists of the following modules: test strips, test kits and the RQflex 10 and RQflex 10 plus reflectometers. No matter whether you work in agriculture, in food or water analysis, in cleaning control, or in industrial analysis with the Reflectoquant system you have an inexpensive, mobile laboratory at your disposal for on-the-spot analysis. The Reflectoquant system offers you numerous tests with a broad range of parameters, measuring ranges and applications for the widest conceivable spectrum of sample materials. Reflectoquant Ascorbic Acid test simplifies the in-process controls for juice production. IQ, OQ and PQ documents for all Reflectoquant Instruments. further details see page

59 The advantages of the Reflectoquant system Small and easy to handle for on-the-spot analysis Barcode calibration for reliable, quantitative results in a matter of minutes Many applications available Low analysis costs Environmentally friendly thanks to a minimal requirement of reagents The Reflectoquant product range at a glance The basic components of a compact solution to analyse more than 20 parameters Instruments RQflex 10 Instrument and basic accessories to measure test strips Test kits Reflectoquant Tests Many parameters need only test strips. If further reagents and tools are needed, they are included in the kits RQflex 10 plus Most flexible instrument to measure test strips and solutions (cell tests) RQeasy RQeasy Nitrat RQeasy Ascorbic Reflectoquant plus Tests Cell test kits for higher sensitivity especially in agricultural and water analysis Reflectoquant Tests RQeasy Test strips designed for application in RQeasy instruments Application instructions Application finder Reflectometry: Tested protocols to prepare food and beverage samples for analysis Quality control Standards Instruction manuals recommend Merck standard solutions (see page 127) or materials to prepare your own standard solution Data transfer RQdata Interface and software package for transfer and documentation of results on IBM-compatible PCs 59

60 Reflectoquant system Fast and inexpensive alternative to supervise raw material ingredients during production Hydroxymethylfurfural (HMF) in honey The amount of hydroxymethylfurfural (HMF) in honey is an important quality criterion. The Reflectoquant HMF test is the very first rapid test for the determination of hydroxymethylfurfural (HMF) content in honey. Check our application HMF in honey which is an alternative to time consuming HPLC analysis for your screening tests. Ascorbic acid in food Ascorbic acid (vitamin C) counts as an essential quality characteristic in many foods. A loss of vitamin C means a deterioration of the food quality which can even be tasted. Check our Reflectoquant Ascorbic Acid Test applications for over 15 sample materials. Reducing (total) sugar in potatoes In fried or backed goods like e.g. potato chips, acrylamide may be produced by the reaction between asparagine and reducing sugars (fructose, glucose, etc.). The recommended upper level of reducing sugars in potatoes should not exceed a set limit. Check our application "Total sugar in potatoes" for Reflectoquant Total Sugar Test. Nitrate in vegetables Nitrate in itself is not harmful to human health. Its metabolic products, on the other hand, can damage health. Limits have been set for nitrate levels in foods, like drinking water, infant foods and vegetables. Check our Reflectoquant Nitrate Test applications for over 15 sample materials. Interested in more application examples? Visit the analytical application finder on our website! 60

61 RQflex 10 / RQflex 10 plus reflectometers RQflex 10 Ord. No Reflectometer for the evaluation of Reflectoquant test strips Scope of delivery and features includes test-strip adapter and recalibration set, double optical system (option for evaluation for two reaction zones), memory for five methods, memory slots for 50 results (with date, time, parameter, and result), interface to PC, batchspecific calibration function (bar-code technology), battery operation with 4 AAA batteries, detailed manual for reflectometer and tests RQflex 10 plus Ord. No Reflectometer for the evaluation of Reflectoquant test strips and Reflectoquant plus test kits Scope of delivery and features as above for Ord.No , also contains cell adapter and eight empty cells RQflex sample preparation Dilution and decoloration Ord. No. Reflectoquant dilution set Polyvinylpolypyrrolidone Divergan RS, 100 g Preserving milk samples Ord. No. Sodium azide tablets, 5000 tabs Potassium dichromate tablets, 5000 tabs RQflex accessories Ord. No. RQdata for RQflex 10 and RQflex 10 plus Interface and software package for transfer and documentation of results on IBM-compatible PCs Test-strip adapter for RQflex 10 and RQflex 10 plus Cell adapter for RQflex 10 plus Recalibration set for RQflex 10 and RQflex 10 plus Empty cells for RQflex 10 plus, 100 disposable cells RQcheck check set for RQflex 10 and RQflex 10 plus

62 Index A-Z Reflectoquant tests Parameter Graduation No. of tests Ord. No. Method Type A Ammonium Test mg/l NH Indophenol blue R Ammonium Test mg/l NH Indophenol blue R Ammonium Test mg/l NH Nessler R Ascorbic Acid Test mg/l ascorbic acid Phosphormolybd. blue Ascorbic Acid Test RQeasy mg/l ascorbic acid Phosphormolybd. blue B Blank Strip C Calcium Test mg/l Ca Glyoxalbishydroxyanil R Calcium Test mg/l Ca Phthaleinkomplexone Chlorine Test mg/l Cl Redox reaction R F Formaldehyde Test mg/l HCHO Triazole R Free Sulfurous Acid Test mg/l SO Nitroprusside/ R Zn-hexacyanoferrate G Glucose Test mg/l glucose Enzymatic reaction H Hydroxymethylfurfural Test mg/l HMF Enzymatic reaction I Iron Test mg/l Fe(II) Triazine Iron Test mg/l Fe(II) Bipyridine L Lactic Acid Test mg/l lactic acid Enzymatic reaction M Magnesium Test mg/l Mg Phthaleinkomplexone Malic Acid Test mg/l malic acid Enzymatic reaction N Nitrate Test 3-90 mg/l NO Modified Griess reaction Nitrate Test mg/l NO Modified Griess reaction Nitrate Test RQeasy mg/l NO Modified Griess reaction Nitrite Test mg/l NO Griess reaction Nitrite Test g/l NO Aromatic amine P Peracetic Acid Test mg/l peracetic acid Redox reaction Peracetic Acid Test mg/l peracetic acid Redox reaction Peroxide Test mg/l H 2 O Enzymatic reaction Peroxide Test mg/l H 2 O Enzymatic reaction ph Test ph Mixed indicator ph Test ph Mixed indicator ph Test for Cooling Lubricants ph Mixed indicator Phosphate Test RQflex plus mg/l PO Phosphormolybd. blue Phosphate Test mg/l PO Phosphormolybd. blue R Potassium Test RQflex plus mg/l K Kalignost, turbidimetric Potassium Test g/l K Dipicrylamine R S Sucrose Test g/l Enzymatic reaction R Sulfite Test mg/l SO Nitroprusside/ Zn-hexacyanoferrate T Total Acidity Test ph g/l (as tartaric acid) Mixed indicator R Total Hardness Test d Phthaleinkomplexone Total Sugar Test mg/l total sugar Enzymatic reaction R (glucose and fructose) U Urea Test in Milk Application mg/l NH Indophenol blue R R incl. reagent 62

63 Agriculture Aquaristics Beverages Biotechnology, fermenter Boiler water, cooling water Construction-material Disinfection control Disposal drainage water Drinking water Electroplating Food Environment Milk, dairy products Groundwater, surface water Mineral water Seawater Swimming pools Wastewater Parameter Ammonium Test Ammonium Test Ammonium Test Ascorbic Acid Test Ascorbic Acid Test RQeasy Blank Strip Calcium Test Calcium Test Chlorine Test Formaldehyde Test Free Sulfurous Acid Test Glucose Test Hydroxymethylfurfural Test Iron Test Iron Test Lactic Acid Test Magnesium Test Malic Acid Test Nitrate Test Nitrate Test Nitrate Test RQeasy Nitrite Test Nitrite Test Peracetic Acid Test Peracetic Acid Test Peroxide Test Peroxide Test ph Test ph Test ph Test for Cooling Lubricants Phosphate Test RQflex plus Phosphate Test Potassium Test RQflex plus Potassium Test Sucrose Test Sulfite Test Total Acidity Test ph 7.0 Total Hardness Test Total Sugar Test (glucose and fructose) Urea Test in Milk Application 63

64 "You can t always trust what you see. So we trust Spectroquant test kits instead." Maria G., laboratory head Quality control is like a final exam: you simply can t afford to make mistakes. So when Maria and her team check key parameters in waste water or drinking water, they rely on Spectroquant test kits. For validated, documented peace of mind and full marks every time. 64

65 Photometry Spectroquant General information 66 Instruments 68 Picco - colorimeters 70 Multy - colorimeter 73 NOVA - photometers 74 Pharo - spectrophotometers 76 Sample preparation 80 Thermoreactors 82 Test kits 86 Test kits - index 88 Test kits for testing seawater 106 Test kits for Hach instruments 110 Analysis methods for the brewery industry 114 Spectroquant Quality 116 Spectroquant Quality assurance

66 Photometric measurements Spectroquant Safety in water analysis Today quality assurance is a decisive factor in water and wastewater analysis after all, the results must be reliable. Only by ensuring start-to-finish quality-assurance your measurements can be counted as secure and reproducible, and are recognized as analytical results. Our Spectroquant analysis system and the components that make up our professional AQA concept make your Internal Quality Control (IQC) operations reliable and secure. The Spectroquant analysis system: user-friendly cell and reagent tests unmistakeable thanks to barcode identification 66

67 The Spectroquant system at a glance Instruments and accessories Spectroquant colorimeters and photometers combine high measurement quality with simple handling Spectroquant Picco Spectroquant Multy Spectroquant NOVA Spectroquant Pharo Sample preparation Correct and comfortable with crack sets and thermoreactors Spectroquant Crack Set Spectroquant Thermoreactors TR 320 TR 420 TR 620 Test kits More than 150 Spectroquant test kits offer competent solutions for the widest conceivable range of applications Reagent test kits Cell test kits Test kits for testing seawater Test kits for Hach instruments Quality certificates Quality certificates for each test kit confirm the consistent quality of all batches Quality assurance Perfect Analytical Quality Assurance (AQA) thanks to certified standards, GLP-compliant documentation, and tools Spectroquant PhotoCheck CertiPUR UV/VIS standards Spectroquant PipeCheck Spectroquant CombiCheck Standard solutions (CRM) for photometric applications CertiPUR standard solutions 67

68 Spectroquant instruments The suitable instrument for each application From simple pocket instruments all the way to the powerful Lab bench photometer our product range offers the appropriate measurement instrument for every area of application. The instruments that make up the Spectroquant colorimeter series are robust, easy to operate, and excellently suited for on-the-spot use. If you need to determine just one or two parameters, then the Picco colorimeter is the instrument of choice for you. For users who prefer a comprehensive and mobile solution, Merck has developed the Spectroquant Multy colorimeter. A high measurement quality coupled with ease of handling and start-to-finish analytical quality assurance these are the outstanding features of the Spectroquant NOVA and Pharo photometers. To complement the robust NOVA filter photometers, the Spectroquant family has been extended to include the Pharo spectrophotometers for universal application. Both types of instruments, NOVA and Pharo, use the practical barcode reading system for both cell and reagent tests, thus dispensing with the need to enter the applicable method manually. What s more, the cell formats are automatically identified and the correct measuring range is selected accordingly. The proven instrument-supported Analytical Quality Assurance system (AQA) is of particular benefit in ensuring GLP-compliant operations. Due to the consistently high quality of the Spectroquant tests it is not even necessary to prepare a blank sample the blank value has already been accounted for in the programming. 68

69 Spectroquant colorimeters and photometers at a glance Spectroquant Picco the mobile solution in a practical carrycase Spectroquant colorimeter Compact instrument for the measurement of Barcode reading Number of test param. Picco Cl 2 / O 3 / ClO 2 / CyA / ph chlorine, ozone, chlorine dioxide, cyanuric acid, ph 6 Mains operation Batteryoperation Picco COD / CSB COD (chemical oxygen demand) 1 Picco F fluoride 1 *after decomposition Spectroquant Multy compact and powerful Spectroquant colorimeter Barcode reading Number of test param. Mains operation Batteryoperation Multy Compact and portable for routine measurements of different parameters > 130 Spectroquant NOVA the robust filter photometer Spectroquant photometer Barcode reading Number of test param. Mains operation Batteryoperation Spectroquant NOVA 30 A Basic instrument for routine measurements in wastewater > 60 Spectroquant NOVA 60 Routine measurements for drinking and wastewater and all other water types > 170 Spectroquant NOVA 60 A As for NOVA 60, can also be operated with rechargeable powerpack > 170 Spectroquant Pharo the versatile spectrophotometer Spectroquant spectrophotometer Spectroquant Pharo 100 Spectrophotometer for all routine measurements as well as for individual applications in the VIS area Barcode reading Number of test param. > 170 Mains operation Batteryoperation Spectroquant Pharo 300 Spectrophotometer for all routine measurements as well as for individual applications in the UV / VIS area > 170 Possible with optional 12V adaptor. * 69

70 Spectroquant instruments Spectroquant Picco colorimeters Spectroquant Picco colorimeters are extremely easy to operate and are preprogrammed for the use of Spectroquant test kits. And because every stage of the working procedure is described in an easy-to-under stand manner, even untrained staff can operate Spectroquant Picco instru ments without any problem. If you need to determine just one or two parameters, then the Picco colorimeter is the instrument of choice for you. General technical data for all Picco colorimeters: Optics temperature-compensated LED and photosensor amplifier Sample Chamber waterproof Power supply 9 V battery, providing 40 h of operation (equivalent to approx. 600 measurement cycles of 4 minutes) Auto-OFF automatic switch-off Dimensions case 270 x 225 x 80 mm (L x W x H) Dimensions instrument 190 x 110 x 55 mm (L x W x H, without adapter) Weight instrument 0.4 kg Ambient temperature 0 C to +40 C Allowable relative humidity %, non condensing CE-conformity DIN EN , VDE 0839 part , DIN EN , VDE 0839 part Features: Water proofed, backlit display, real-time-clock and date storage function for measurement results, absorption measurement 70

71 Spectroquant Colorimeter Picco Cl 2 / O 3 / ClO 2 / CyA / ph Ord. No for the determination of free and total chlorine, ozone, chlorine dioxide, cyanuric acid and ph Delivery incl. Case with photometer, 9 V battery, adapter for 16 mm round cells, lid for adapter, 3 x 24 mm round cells, operating manual Measuring wavelength Measuring time Auto-OFF 528 nm (LED plus filter) 3-4 seconds 8 minutes after last key press Spectroquant test kit Measuring range in mg/l Number of tests Ord. No. Chlorine Test, free Chlorine Test, free Chlorine Test, total Chlorine Test, total Chlorine Test, free and total Chlorine Reagent 1 (liquid) Free Chlorine: Use Reagent 1+2 Chlorine Reagent 2 (liquid) Total Chlorine: Use Reagent Chlorine Reagent 3 (liquid) Ozone Test Ozone Test Chlorine Dioxide Test Cyanuric Acid Test ph Cell Test ph Absorbance ma Accessory for Analytical Quality Assurance: Spectroquant Reference Standard for chlorine, chlorine dioxide and ozone Ord. No The Spectroquant Reference Standard is used to carry out checks of accuracy and reproducibility of measuring results only with the Spectroquant Picco Cl 2 /O 3 /ClO 2 /CyA/pH colorimeter and the Spectroquant Multy colorimeter. The standards are supplied in sealed vials and are individually calibrated on instruments traceable to N.I.S.T. SRM 2032, 935a. The kit contains one Zero standard and 3 cuvettes for checking 3 different concentrations according to the appropriate values for each parameter: Chlorine mg/l Chlorine Dioxide mg/l Ozone mg/l 71

72 Spectroquant instruments Spectroquant Colorimeter Picco COD / CSB Ord. No for the determination of COD Delivery incl. Case with photometer, 9 V battery, adapter for 16 mm round cells, lid for adapter, operating manual Measurement accuracy ±3.5 % Measuring wavelength 430 nm (LED plus filter) and 605 nm (LED) Measuring time 3-4 seconds Auto-OFF 8 minutes after last key press Spectroquant test kit Mesuring range Number of tests Ord. No. COD Cell Test mg/l COD Cell Test mg/l COD Cell Test mg/l COD Cell Test g/l Absorbance ma Remark: For the digestion of COD a thermoreactor is required. Details see in chapter Spectroquant Thermoreactors. Spectroquant Colorimeter Picco F Ord. No for the determination of fluoride Delivery incl. Case with photometer, 9 V battery, adapter for 16 mm round cells, lid for adapter, 3 x 16 mm empty round cells, operating manual Measurement accuracy ±3.5 % Measuring wavelength 620 nm (LED) Measuring time 3-4 seconds Auto-OFF 15 minutes after last key press Spectroquant test kit Measuring range in mg/l Number of tests Ord. No. Fluoride Test Fluoride Test Fluoride Cell Test μg/l Accessories for Spectroquant Picco colorimeters and Multy colorimeter Empty cells 24 mm with screw caps package of 12 pcs Ord. No Empty cells 16 mm with screw caps package of 25 pcs Ord. No

73 Spectroquant Multy colorimeter The Spectroquant Multy colorimeter is the ideal instrument for all users looking for an inexpensive and allround solution for photometric water analysis. Over 130 methods on the basis of the established Spectroquant test kits cover all essential parameters in the field of drinking-water and wastewater analysis. The included rechargeable batteries and the practical carrycase enable the instrument to be used anywhere, anytime. Thanks to the battery-charging feature integrated in the instrument, the batteries can be recharged while running the device off the mains power supply. The connection port for an optional printer or PC also makes it possible to transmit sets of measurement data and to download new software updates via the internet effortlessly. Spectroquant Colorimeter Multy Ord. No Scope of delivery Display Optics Measuring wavelength Interface Methods Keypad Power Supply Ambient Conditions System check Storage capacity CE approval Dimensions Case, colorimeter, adapter unit for 16 mm round cells, lid for adapter unit, 7 rechargeable batteries, lithium battery (to ensure data storage), interface cable for connection to the PC or printer, 3 x 16 mm round cells, 3 x 24 mm round cells, screw driver (for battery compartment), 100 ml plastic beaker, operating manual Large format graphic display 6 temperature compensated LED with interference filters, internal reference channel (dual beam technology) 430 nm, 530 nm, 560 nm, 580 nm, 610 nm, 660 nm RS 232 for printer and PC connection Programming of more than 130 methods for Spectroquant cell and reagent tests, as well as physical measurements and preprogrammed applications Acid and solvent resistant, touch-sensitive with audible feedback 7 Ni-MH-battery pack (AA/Mignon), charged whilst in the unit with integrated mains charger, integrated overload cut-out. up to max. 90 % humidity (non condensing), approx C automatic selfcheck of the instrument for 1000 data sets, with date, time and registration number yes approx. 265 x 195 x 70 mm (unit), 440 x 370 x 140 mm (case) Spectroquant Verification Standard For accuracy and reproducibility checks Ord. No further details see page

74 Spectroquant instruments Spectroquant NOVA 30 A The Spectroquant NOVA 30 A, the basic instrument for comfortable wastewater analysis, is compact, robust, and whenever the circumstances demand mobile, too. This instrument is capable of running all important cell tests for waste-water ana lysis. It goes without saying that even this basic model incorporates advanced features such as the barcode reading system, AQA support, and documentation. Spectroquant Photometer NOVA 30 A Ord. No Net version, incl. rechargeable battery, graphic display 128 x 64 pixel Wavelength 6 filters in array-technique with reference beam: 340, 445, 525, 550, 605, 690 nm ±2 nm, half band width 10 nm (30 nm for 340 nm) Photometric reproducibility A at A Photometric resolution A Types of determination Absorbance, concentration, transmission Measur. range of absorbance A to A Lamp Tungsten halogen lamp, preset, no warm-up time, measuring time 2 s Date/Time real time clock integrated in the photometer Cell compartment 16 mm ø cells Test recognition AutoSelect function (bar-code reading system) automatic cell recognition Method-update via Internet AQA 3 quality control modes Turbidity correction simultaneous multiwavelength measurement to correct turbidity Interface RS 232 C serial interface for printer and computer Methods Programming of more than 60 methods for Spectroquant cell tests, as well as physical measurements and preprogrammed applications Storage capacity up to 500 results Power supply V~, Hz Temperature Storage: -25 C to +65 C, operations: +5 C to +40 C Allowable relative humidity Annual mean: 75 %, 30 days/year: 95 %, other days: 85 % Dimensions 140 x 270 x 260 mm (H x D x W) Weight 2.8 kg incl. battery Accessories for NOVA For further information please see page

75 Spectroquant NOVA 60 and 60 A The Spectroquant NOVA 60 is an instrument for the routine analysis of all water types and is capable of measuring both the ready-to-use cell tests as well as inexpensive reagent tests. Over 150 test kits with different measuring ranges and practically relevant forms of reference make the NOVA 60 a photometer with a universal range of applications. The instrument can store up to 50 user-defined methods. The Spectroquant NOVA 60 A can also be used as a mobile analysis station. Spectroquant Photometer NOVA 60 Ord. No Net version, graphic display 128 x 64 pixel Wavelength 12 filters in array-technique with refer. beam: 340, 410, 445, 500, 525, 550, 565, 605, 620, 665, 690, 820 nm ±2 nm, half band width 10 nm (30 nm for 340 nm) Photometric reproducibility Photometric resolution Types of determination Measur. range of absorbance Lamp Date/Time Cell compartment Test recognition Method-update AQA Turbidity correction Interface Methods A at A A Absorbance, concentration, transmission A bis A Tungsten halogen lamp, preset, no warm-up time, measuring time 2 s real time clock integrated in the photometer 10, 20, 50 mm cuvettes and 16 mm ø cells AutoSelect function (barcode reading system) automatic cell recognition via Internet 3 quality control modes simultaneous multiwavelength measurement to correct turbidity RS 232 C serial interface for printer and computer Programming of more than 170 methods for Spectroquant cell and reagent tests, as well as physical measurements and preprogrammed applications Storage capacity up to 1000 results Special functions 50 free programmable methods Power supply V~, Hz Temperature Storage: -25 C to +65 C, operations: +5 C to +40 C Allowable relative humidity Annual mean: 75 %, 30 days/year: 95 %, other days: 85 % Dimensions 140 x 270 x 260 mm (H x D x W) Weight 2.3 kg Spectroquant PhotoCheck For checking the photometer, further details see page 119. Spectroquant Photometer NOVA 60 A Ord. No Net version incl. rechargeable battery, graphic display 128 x 64 pixel All technical details same as NOVA 60 (Ord. No ) Weight 2.8 kg incl. battery 75

76 Spectroquant instruments The Spectroquant Pharo range of spectrophotometers The new Spectroquant Pharo 100 and 300 spectrophotometers combine the advantages of a system photometer such as test kits optimally adapted to the instrument with those of a spectrophotometer. No matter whether you wish to programme your own methods, record spectra or kinetic profiles, or make multi-wavelength measurements here everything is right at your fingertips. In addition you can make use of all the advantages of the established Spectroquant system, such as instrument-supported Analytical Quality Assurance (AQA) and the barcode scanning system for both cell and reagent tests. The adapterless, automatic identification of all types and formats of cells, and also the intuitive menu interface and the large-format display make the instrument s operation child s play. USB and RS-232 interfaces enable the simple transfer of data to printers, PCs or in wireless mode to a USB stick. The Pharo spectrophotometers also support the user in ensuring GLP-compliant operations. 76

77 Spectroquant Pharo 100 The Spectroquant Pharo 100 spectrophotometer with a wavelength spectrum from 320 to 1100 nm is well suited for all routine measurements as well as for individual use in the VIS range. Combined with the barcode scanning system as well as AQA support and documentation, the Pharo 100 is truly a photometer with universal applications. Spectroquant Spectrophotometer Pharo 100 Ord. No Spectrophotometer with plug-in power supply, backlit graphic display Wavelength range nm Technology Stabilized single-beam Source lamp Tungsten halogen lamp Measuring modes Concentration, absorbance, transmission, multi wavelengths, scans and kinetics in absorbance or transmission mode Spectral bandwidth 4 nm Wavelength resolution 1 nm Wavelength accuracy ± 1 nm Photometric range ± 3.3 A Absorbance resolution A Absorbance accuracy A bei < A; 0.5 % of the reading for A 2,000 Scan Scans in 1 nm increments with free selectable wavelength range Cells 16 mm round, 10, 20, 50 mm rectangular with automatic cell size recognition Test recognition Autom. bar-code reading system for all Spectroquant cell and reagent tests Method update via Internet / PC or via USB stick Instrument-supported quality assurance AQA 1: Instrument check using PhotoCheck and/or CertiPUR UV/VIS standards AQA 2: System check using CombiCheck resp. CertiPUR standards AQA 3: Testing samples for interferences by means of the MatrixCheck function Communication interfaces 1 USB-A, 1 USB-B, 1 RS 232 Data storage 1000 single measured values; 4 MB for scans and kinetics, i.e. approx. 100 scans ( nm) and 400 kinetic curves with 150 single values each Languages Methods and profiles German, English, Spanish, French, Italian, Brazilian-Portuguese, Bulgarian, Chinese (simplified), Chinese (traditional), Czech, Greek, Hungarian (Magyar), Indonesian, Japanese, Malay, Mazedonian, Norwegian, Polish, Portuguese (see Brazilian-Portuguese), Russian, Slowenian, Thai* Programmed methods of all Spectroquant cell and reagent tests, 100 userdefined methods, 20 profiles for kinetic and absorption scans each Protection class IP 30 Power supply Country specific power supply, length of the connection cable: 2.0 m Power requirements V ~ / Hz / 0.70 A Temperature Operating: +10 C to +35 C, Storage: -25 C to +65 C Allowable relative humidity Annual mean: 75 %; 30 days / year: 95 %; other days: 85 % Dimensions 404 x 197 x 314 mm (width x height x depth) Weight (without plug-in power supply) approx. 4.5 kg *please contact your Merck representative for availability of additional languages Spectroquant PhotoCheck For checking the photometer, further details see page

78 Spectroquant instruments Spectroquant Pharo 300 All functions of the Spectroquant Pharo 100 plus an additional wavelength spectrum from 190 to 1100 nm make the Spectroquant Pharo 300 an instrument with a virtually limitless range of applications, enabling to carry out all your measurements with ease and comfortably. Spectroquant UV/VIS Spectrophotometer Pharo 300 Ord. No Spectrophotometer with plug-in power supply for all routine measurements and for individual use in the UV/VIS range, backlit graphic display Wavelength range Technology Source lamp Measuring modes nm Stabilized single-beam Xenon flashlamp Concentration, absorbance, transmission, multi wavelengths, scans and kinetics in absorbance or transmission mode Spectral bandwidth 4 nm Wavelength resolution 1 nm Wavelength accuracy ± 1 nm Photometric range ± 3.3 A Absorbance resolution A Absorbance accuracy A bei < A / 0.5% of the reading for A Scan Scans in 1 nm increments with free selectable wavelength range Cells 16 mm round, 10, 20, 50 mm rectangular with automatic cell size recognition Test recognition Automatic bar-code reading system for all Spectroquant cell and reagent tests Method update via Internet/PC or via USB stick Instrument-supported quality assurance AQA 1: Instrument check using PhotoCheck and/or CertiPUR UV/VIS standards AQA 2: System check using CombiCheck resp. CertiPUR standards AQA 3: Testing samples for interferences by means of the MatrixCheck function Communication interfaces 1 USB-A, 1 USB-B, 1 RS 232 Data storage 1000 single measured values; 4 MB for scans and kinetics, i.e. approx. 100 scans ( nm) and 400 kinetic curves with 150 single values each Languages Methods and profiles German, English, Spanish, French, Italian, Brazilian-Portuguese, Bulgarian, Chinese (simplified), Chinese (traditional), Czech, Greek, Hungarian (Magyar), Indonesian, Japanese, Malay, Mazedonian, Norwegian, Polish, Portuguese (see Brazilian-Portuguese), Russian, Slowenian, Thai* Programmed methods of all Spectroquant cell and reagent tests, 100 userdefined methods, 20 profiles for kinetic and absorption scans each 78 Protection class IP 31 Power supply Country specific power supply, length of the connection cable: 2.0 m Power requirements V ~ / Hz / 0.70 A Temperature Operating: +10 C to +35 C, Storage: -25 C to +65 C Allowable relative humidity Annual mean: 75%, 30 days / year: 95%, other days: 85% Dimensions 404 x 197 x 314 mm (width x heigth x depth) Weight (without plug-in power supply) approx. 4.5 kg *please contact your Merck representative for availability of additional languages Spectroquant PhotoCheck CertiPUR UV/VIS standards For checking the photometer, further details see page 119.

79 Accessoires for: Spectroquant NOVA photometers Cases Ord. No. Case for Spectroquant NOVA 30 and NOVA 60 photometers PC software and cable Ord. No. Multi-Achat II for Windows (German Version + English Version on CD-ROM) PC software for transferring data from the Spectroquant NOVA 30, NOVA 60, and NOVA 400 photometers. Additional tool enables control WTW ph, oxygen and conductivity meters. System requirements: Windows 98/2000/XP/WIN 7 (32-Bit) PC cable for Spectroquant NOVA 30, NOVA 60, NOVA 400 photometers (for serial bus) Halogen lamps Ord. No. Halogen lamp for Spectroquant NOVA 30 and NOVA 60 photometers Halogen lamp for Spectroquant NOVA 400 spectrophotometer Spectroquant Pharo spectrophotometers Cases and cables for portable use* Ord. No. Halogen lamp modul for Spectroquant spectrophotometer Pharo Case for Spectroquant spectrophotometer Pharo 100 and Pharo V adapter for Spectroquant spectrophotometer Pharo 100 and Pharo 300 (car, PowerPack) * For the mobile use of the Spectroquant Pharo spectrophotometers we recommend using a PowerPack rechargeable battery with a 12 V plug adapter as the power source. Spectroquant photometer (for use with the NOVA and Pharo instruments) Rectangular and round cells Ord. No. Rectangular cells 10 mm (1 pack = 2 pcs) Rectangular cells 20 mm (1 pack = 2 pcs) Rectangular cells 50 mm (1 pack = 2 pcs) Semi-microcells 50 mm (1 pack = 2 pcs) Rectangular cells quartz 10 mm (1 pack = 2 pcs) Empty cells 16 mm ø (1 pack = 25 pcs) with screw cap Positioning Aid for 10 mm plastic cuvette Cleaning agents for cells and glass vessels. For more information see page

80 Spectroquant sample preparation Simply effective Sample preparation is an integral component of the Spectroquant analysis system. Some test kits already contain all the reagents necessary for preparing the sample material for analysis. For a broad range of other analytical tasks we also offer Spectroquant Crack Sets for simple and effective sample pretreatment. Samples can then be effortlessly decomposed in the thermoreactors. COD, TOC, AOX, total nitrogen, total phosphorus, total chromium, and silver can be swiftly and easily determined with our tests. The test kit contains all the necessary reagents even those required for the decomposition. The illustrated working instructions guide you safely to the correct result. The reagents contained in the package and the analysis instructions accessible at make sample digestion a child s play. 80

81 Simply correct Crack Sets It s not always a matter of determining the total content. This is why decomposition reagents for sample preparation are also available as separate packs. In this way you retain your working flexibility, while at the same time you save having to order all the chemicals in the test kit. Crack Set 10 for 100 digestions Ord. No The Crack Set 10 for 100 decompositions contains digestion reagents for the determination of the total content of cadmium, chromium, copper, iron, lead, nickel, phosphorus, and zinc for use in conjunction with the thermoreactor. Contents: digestion reagent, acid, neutralizing agent for ph adjustment. Crack Set 10 C for 25 digestions Ord. No The "C" stands for cells. This means that the cells necessary for the decomposition in the thermoreactor are contained in the set. The digestion reagent is predosed, and the set s application is identical to that of the Crack Set 10. Contents: digestion reagent filled into round cells, acid, neutralizing agent for ph adjustment. Crack Set 20 for 90 digestions Ord. No The Crack Set 20 contains digestion reagents for the determination of total nitrogen for use with the thermoreactor. Contents: digestion reagent, lye. When the objective is to determine the total content of certain parameters, the Crack Sets contain all the necessary reagents. 81

82 Spectroquant sample preparation Simply comfortable Spectroquant thermoreactors Developed in practice for practice, we offer a system of thermoreactors that fulfils every conceivable requirement. The standard digestion programmes Standard programmes for routine digestions help avoid operating mistakes from occurring in the first place. The Spectroquant thermoreactor family features eight standard programmes for day-to-day routine use: 148 C, 120 min for COD 148 C, 20 min for COD (rapid digestion method) 150 C, 120 min for COD acc. to USEPA 120 C, 120 min for TOC 120 C, 60 min for total nitrogen, total contents of Cr, Cu, Ni, Pb, Cd, Fe, and Zn 120 C, 30 min for AOX and total phosphorus, cyanide 100 C, 60 min for Ag 100 C, 30 min A description of the digestion procedures is already integrated in the instruction sheets included with the test kits. For special digestion variants there are applications available as downloads from the internet. 82 The standard digestion programmes are already stored in the thermoreactors. The TR 420 and TR 620 thermoreactors are capable of digesting as many as 24 samples at the same time.

83 The advantages of the Spectroquant thermoreactors Practically oriented tuning of the digestion reagents to the thermoreactors Simple handling thanks to user-friendly description of the digestion procedures Flexible selection between standard programmes and individual programming Time-saving COD rapid digestion method Thermoreactors at a glance Spectroquant TR 320 thermoreactor the starter model Instrument Functions Area of application Spectroquant TR stored standard programmes Simultaneous digestion of 12 samples The instrument features all you need for handling digestions simply and correctly. Spectroquant TR 420 thermoreactor the advanced instrument for experts Instrument Functions Area of application Spectroquant TR 420 Free temperature and time selection 8 stored standard and 8 free programmes Simultaneous digestion of 24 samples Thermosensor and PC cable available AQA documentation for control purposes Individual programming for future assignments The instrument already has all digestion programmes necessary for wastewater analysis preinstalled, but can also be individually used in the temperature range up to 170 C and with digestion times of up to three hours. Spectroquant TR 620 thermoreactor the two-in-one instrument for professional use Instrument Functions Area of application Spectroquant TR 620 as for the TR 420 thermoreactor, but with two separate temperature-selectable heating zones As a two-in-one professional model this instrument features all the advantages of the TR 420. It has two separate controllable heating blocks, which makes it possible to run different digestion programmes at one and the same time. It is e.g. capable of simultaneously digesting 12 COD and 12 TOC samples at the different temperatures required, yielding results after just 120 minutes. 83

84 Spectroquant sample preparation General technical data for all thermoreactors: incl. integrated protective hood for the determination of COD and TOC, as well as of total contents of cadmium, chromium, copper, cyanide, iron, lead, nickel, nitrogen, phosphorus, silver, and zinc. Display LCD display for temperature and time, desired and actual values for heating time and temperature continually shown in the LCD display Heater On/off display (the LED blinks red during the heating phase and is permanently on during the digestion phase), contact guard on the surface of the heating-block Spectroquant Thermoreactor TR 320 Ord. No Holes 12 for cell tests ø 16 mm Temperature selection 100 C, 120 C and 148 C ±1.0 C Controlling accuracy ±1 C ±1 digit Heating time 8 temperature heating-time programs for simplest possible operation: 148 C (20 min or 120 min), 150 C (120 min), 120 C (30 min, 60 min or 120 min), 100 C ( min) automatic power switch-off at the end of the heating time Mains version 115 V~ / 230 V~, 50 Hz / 60 Hz convertible Dimensions 180 x 256 x 307 mm (H x W x D) Weight 2.85 kg Spectroquant Thermoreactor TR 420 Ord. No Holes 24 for cell tests ø 16 mm Temperature selection Room temperature -170 C ±1.0 C Controlling accuracy ±1 C ±1 digit Timer min freely selectable Heating time 8 temperature heating-time programmes for simplest possible operation: 148 C (20 min or 120 min), 150 C (120 min), 120 C (30 min, 60 min or 120 min), 100 C ( min), as well as eight freely selectable programmes, automatic power switch-off at the end of the heating time Optional accessories Thermosensor: heating-block temperature-monitoring option via integrated serial interface and control software for AQA, brass adapter with integrated Pt sensor fitting the holes incl. connector cable (for checking equipment) Mains version 115 V~ / 230 V~, 50 Hz / 60 Hz convertible Dimensions 180 x 256 x 307 mm (H x W x D) Weight 3.6 kg 84

85 Spectroquant Thermoreactor TR 620 Ord. No two separate temperature-selectable heating zones, incl. integrated protective hood for the determination of COD and TOC, as well as of total contents of cadmium, chromium, copper, cyanide, iron, lead, nickel, nitrogen, phosphorus, silver, and zinc. Holes 24 (2 x 12) for cell tests ø 16 mm, the temperature of each of the two heating zones can be set and controlled separately Temperature selection Room temperature C ±1.0 C Controlling accuracy ±1 C ±1 digit Timer min freely selectable Heating time 8 temperature heating-time programmes for simplest possible operation: 148 C (20 min or 120 min), 150 C (120 min), 120 C (30 min, 60 min or 120 min), 100 C ( min) as well as eight freely selectable programmes, automatic power switch-off at the end of the heating time Optional accessories Thermosensor: heating-block temperature-monitoring option via integrated serial interface and control software for AQA, brass adapter with integrated Pt sensor fitting the holes incl. connector cable (for checking equipment) Mains version 115 V~ / 230 V~, 50 Hz / 60 Hz convertible Dimensions 180 x 256 x 307 mm (H x W x D) Weight 3.6 kg Accessories for Spectroquant thermoreactors Thermosensor for thermoreactors TR 420/620 Ord. No The thermosensor measures the current temperature in the bore of the thermoreactor and compares it with the specified temperature. The results can be transmitted to a PC for documentation purposes. PC cable for thermoreactors TR 420/620 Ord. No

86 Spectroquant test kits Validation provides security All Spectroquant analysis methods are validated. Validation data are registered for each single Spectroquant test kit and recorded in the validation document. Many Spectroquant analysis methods conform with international standards and are thus comparable with references. This gives you the sense of security you need in your operations. Reagent test kits Spectroquant reagent test kits contain highly stable, ready-to-use reagent mixtures in a userfriendly design. Selection of the appropriate cell format makes it easy to vary the measuring range as you require. We check the linearity of the calibration function for all cell formats. Each pack contains an AutoSelector that gives the barcode for the selection of the correct method in the photometer. The lower limit of detection (LLD) can be extended in many cases into the ppb range by the use of 50 mm cells. Spectroquant test kits can be used in conjunction with all spectrophotometers and also with photometers from other suppliers. The reagents are either in liquid or in powder form and are added to the sample using a dropper, pipette, or metering spoon. The outstanding features of these products are the extremely long shelf-life (up to three years) and the avoidance of the necessity for storage in a refrigerator. The AutoSelector is an integral component of each and every reagent test set and enables the automatic selection of the correct method by barcode identification. 86

87 The advantages of the Spectroquant test kits Validated and standard-compliant analysis reagents for your sense of security Bar-code system enables rapid and simple operation Free selection between cell and reagent tests helps contain costs Comprehensive documentation of the quality control for every test Batch and quality certificates downloadable from our website Cell test kits The characteristic feature of the Spectroquant cell test kits is their high user-friendliness. Virtually all of the reagents necessary for the analysis are contained in cells. The barcode on each of these cells ensures that no mistakes can occur. When you insert your cell into a Spectroquant NOVA or Pharo photometer, the instruments auto matically select the correct method of analysis. As you can see mistakes are fully excluded. The packaging concept offers the cells ideal protection from fluctuations in temperature and moisture during transport. The external label states important information on the contents and also gives data regarding safety and risk issues, the batch number, and the shelf-life of the test kit (up to three years). The package insert provides an easy-to-survey presentation of all relevant information for example the reaction principle, an exact description of the working procedure, area of application, the influence of foreign ions, and on the accuracy. The cell label with the barcode shows the parameter and item number, risk phrases, and the contents and barcode identification a whole lot of information in just a little space. Reagents that must be added for the determination or for the pretreatment of the sample are contained in easy-to-handle dose-metering bottles. 87

88 Index A Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests A Absorbance A A A Acid Capacity Cell Test to ph 4.3 (total alkalinity) 3) mmol/l mmol/l mmol/l CaCO Alkalinity (total) Aluminium Cell Test Al Aluminium Test μg/l Al Ammonium Cell Test μg/l μg/l NH 4 -N NH 4 Ammonium Test NH 4 -N 250 NH Ammonium Cell Test NH 4 -N NH 4 Ammonium Cell Test Ammonium Test NH 4 -N NH 4 Ammonium Cell Test NH 4 -N NH 4 Antimony Sb AOX Cell Test AOX AOX Sample Preparation Set AOX Enrichment Set AOX Standard mg/l Arsenic Test μg/l As Arsenic reagent 2: Sulfuric Acid % GR Arsenic reagent 7: Zinc granular GR, particle size 3-8 mm, ISO Absorption Tube for Arsenic with ground joint NS useable in NOVA 30 3) The cell test contains three round-bottomed cells each bearing a barcode label. The sample is prepared and measured in the round-bottomed cell. After measurement the round-bottomed cells can be emptied and cleaned for subsequent measurements. The round-bottomed cells can thus be used several times. 88

89 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA/Pharo [mg/l] [mg/l] application own coloring physical measurement - 10, 20, 50 Indicator mmol/l Chromazurol S Chromazurol S +/ mmol/l 2,5, 9, 10, 11, 13, 15, 18 see Acid capacity Cell Tests to ph 4.3 6, 16, 17 analogous DIN ISO E30 analogous DIN ISO E30 Indophenol blue analogous EPA 350.1, APHA 4500-NH 3 F, ISO 7150/1, DIN E / , 6, 8, 9, 11, 13, 15, 17, , 20, / , 6, 9, 11, 13, 15, 16, 17, / , 2, 9, 11, 13, 15, 17, 18 Indophenol blue analogous EPA 350.1, APHA 4500-NH 3 F, ISO 7150/1, DIN E5 Indophenol blue analogous EPA 350.1, APHA 4500-NH 3 F, ISO 7150/1, DIN E5 Indophenol blue analogous EPA 350.1, APHA 4500-NH 3 F, ISO 7150/1, DIN E5 Indophenol blue analogous EPA 350.1, APHA 4500-NH 3 F, ISO 7150/1, DIN E5 Indophenol blue analogous EPA 350.1, APHA 4500-NH 3 F, ISO 7150/1, DIN E , 20, / , 2, 9, 11, 13, 15, 17, / , 2, 6, 8, 9, 11, 13, 15, 16, / , 6, 8, 11, 13, 16, / / , 4, 8, 12, / , 4, 8, 16, 18 Application Iron(III)-thiocyanate / , 8, 18 additionally required for AOX measurement for multiple use, additionally required for AOX measurement for 8 16 quality tests, analogous DIN EN ISO 9562 Silver DDTC analogous EPA 206.4, APHA 3500-As, EN additionally required for Arsenic measurement additionally required for Arsenic measurement for multiple use, additionally required for Arsenic measurement 5.0 / , 8, 9, 11, 13, 15, (+ 350) 10, / , 8, 11, 13, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 89

90 Index B-C Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests B BOD Cell Test 3) BOD BOD (Oxygen) Reaction bottle BOD Nutrient Salt Mixture (with allyl thiourea) 12 l BOD Standard 210 +/- 20 mg/l 10 l Boron Test B Boron Cell Test B Bromate BrO 3 Bromine Test Br C Cadmium Test 2) μg/l Cd Cadmium Cell Test 2) μg/l Cd Calcium Test Ca Calcium Test Ca CaO CaCO 3 Ca CaO CaCO Calcium Cell Test Ca CaO CaCO Carbohydrazide Chloride Test Cl Chloride Cell Test Cl Chlorine Test (free chlorine) Cl USEPA approved 1b) Chlorine Cell Test 3) (free chlorine) Cl USEPA approved 1b) Chlorine Test (total chlorine) Cl USEPA approved 1c) useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 3) The cell test contains three round-bottomed cells each bearing a barcode label. The sample is prepared and measured in the round-bottomed cell. After measurement the round-bottomed cells can be emptied and cleaned for subsequent measurements. The round-bottomed cells can thus be used several times.

91 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA/Pharo [mg/l] [mg/l] application mod. Winkler method / , 8, 9, 11, 16, 18 4 bottles are necessary for 1 determination, 6 for 2, 8 for 3 etc. for 12 x 1 l nutrient salt solution, additionally required for BOD measurement, anal. EN for 10 x 1 l standard solution, analogous EN 1899 Rosocyanine analogous EPA 213.3, APHA 4500-B B / , 9, 13, 15, 18 Azomethine H analogous DIN D / , 9, 13, 15, 16 Application , 9, 13, 15 DPD 10 10, 20, / , 7, 9, 17 Cadion derivative , 20, / , 8, 9, 10, 11, 13, 15, 18 Cadion derivative / , 8, 9, 10, 11, 13, 15, 16, 18 Phthalein derivate / , 3, 8, 9, 11, 12, 13, 16, 19 Glyoxalbishydroxyanil , /- 4 1, 2, 5, 6, 9, 13, 15, 16, Phthaleinkomplexone for determinations in the low measuring range see manual NOVA / Pharo / /- 9 1, 2, 5, 6, 9, 13, 15, 16, see Oxygen Scavengers Test Iron(III)-thiocyanate analogous EPA 325.1, APHA 4500-Cl- E Iron(III)-thiocyanate analogous EPA 325.1, APHA 4500-Cl- E DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO / / , 5, 6, 8, 9, 10, 12, 13, 15, 16, /- 5 2, 5, 6, 8, 9, 10, 12, 13, 15, 16, , 20, / , 5, 7, 9, 12, 13, 17, / , 5, 7, 9, 12, 13, 17, , 20, / , 17, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 91

92 Index C Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests C Chlorine Test 100 tests free chlorine tests chlorine (total) USEPA approved 1c) Chlorine Cell Test 3) 100 tests free chlorine tests chlorine (total) USEPA approved 1c) Cl Cl Chlorine Reagent Cl 2-1 (liquid) 5) Cl Chlorine Reagent Cl 2-2 (liquid) 5) Cl Chlorine Reagent Cl 2-3 (liquid) 5) Cl Cells and accessories for the photometric chlorine measurement with liquid reagents , and Cl Chlorine Dioxide Test ClO Chromate Test 2) for the determination of chromium (VI) μg/l μg/l Cr CrO 4 Chromate Cell Test 2) for the determination of chromium (VI) and chromium (total) USEPA approved 1a) Cr CrO 4 Chromium in electroplating baths g/l g/l CrO 3 COD Cell Test 2) COD USEPA approved 1a) COD Cell Test 2) COD COD Cell Test 2) COD USEPA approved 1a) COD Cell Test 2) COD USEPA approved 1a) useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 3) The cell test contains three round-bottomed cells each bearing a barcode label. The sample is prepared and measured in the round-bottomed cell. After measurement the round-bottomed cells can be emptied and cleaned for subsequent measurements. The round-bottomed cells can thus be used several times. 5) Combination for free or total chlorine: see page 71 92

93 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA /Pharo [mg/l [mg/l] application DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO , 20, / , 7, 9, 17, 18 DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO / , 7, 9, 17, 18 DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO DPD analogous EPA 330.5, APHA 4500-Cl G, DIN EN ISO DPD Measuring range of NOVA 30 is mg/l Cl 2, for free chlorine: Cl 2-1 and Cl 2-2 for total chlorine: Cl 2-1, Cl 2-2 and Cl 2-3 DPD analogous APHA 4500-ClO 2 D, DIN G5 Diphenylcarbazide analogous APHA 3500-Cr D, DIN D24 Diphenylcarbazide analogous APHA 3500-Cr D, DIN D , / , 7, 9, 17, , / , 7, 9, 17, , / , 7, 9, 17, , 20, / , 7, 9, , 20, / , 6, 8, 9, 10, 11, 13, 14, 15, 16, (+10) / , 6, 8, 10, 11, 14, 16, 18 Application , 20, 50 Oxidation with chromosulfuric acid, determination as chromate Oxidation with chromosulfuric acid, determination as chromate Oxidation with chromosulfuric acid, determination as chromate Oxidation with chromosulfuric acid, determination as chromate analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO / , 5, 6, 9, 11, 13, / , 9, 11, 13, /- 7 2, 5, 11, 13, /- 8 2, 11, 13, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 93

94 Index C-D Spectroquant test kits COD Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests C COD Cell Test 2) USEPA approved 1a) COD Cell Test 2) COD USEPA approved 1a) COD Cell Test 2) COD USEPA approved 1a) COD Cell Test 2) COD USEPA approved 1a) COD Cell Test 2) COD COD Cell Test (Hg free) 2) COD COD Cell Test (Hg free) 2) COD Color, true and apparent Pt / Co (CU) Color measurement m m -1 Copper Test Cu Copper Cell Test Cu Copper in electroplating baths g/l g/l Cu Cyanide Test 2) μg/l CN (free and readily liberated cyanide) Cyanide Cell Test 2) (free and readily liberated cyanide USEPA approved 1a) ) μg/l CN D Cyanuric Acid Test Cyanuric acid DEHA (Diethylhydroxylamine) Detergents useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 94

95 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA /Pharo [mg/l] [mg/l] application Oxidation with chromosulfuric acid, determination as chromium(iii) Oxidation with chromosulfuric acid, determination as chromate Oxidation with chromosulfuric acid, determination as chromium(iii) Oxidation with chromosulfuric acid, determination as chromium(iii) Oxidation with chromosulfuric acid, determination as chromium(iii) Oxidation with chromosulfuric acid, determination as chromate Oxidation with chromosulfuric acid, determination as chromium(iii) own coloring own coloring analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO analogous EPA 410.4, APHA 5220 D, ISO /- 29 8, 10, 11, /- 13 2, 11, /- 66 8, 10, 11, / , 3, 8, 10, 11, 12, 14, / , 3, 8, 10, 11, 12, 14, /- 8 9, 11, 13, / , 18 physical measurement (Pt/Co, APHA, Hazen, Color Unit) physical measurement according EN ISO 7887; at 445, 525 and 620 nm with NOVA 60 at 436, 525 and 620 nm with Pharo 100/300 10, 20, Cuprizone , 20, / , 2, 5, 6, 8, 9, 10, 11, 13, 16, 18 Cuprizone / , 2, 5, 6, 8, 9, 10, 11, 13, 16, 18 Application , 20, 50 Barbituric acid, pyridine-carboxylic acid Barbituric acid, pyridine-carboxylic acid analogous EPA 335.2, ISO 6703; DIN D13 and D14 analogous EPA 335.2, ISO 6703, DIN D13 and D (+ 10) 10, 20, / , 9, 10, 11, 13, 15, (+ 10) / , 10, 11, 13, 15, 18 Turbidity /- 5 7, 11, 17 see Oxygen Scavengers Test see Surfactants Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 95

96 Index F-M Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests F Fluoride Cell Test F Fluoride Cell Test F Fluoride Test 4) F Formaldehyde Test HCHO Formaldehyde Cell Test HCHO G Gold Test Au H Hardness Hazen Color Number (Pt/Co / APHA / Hazen) Pt/Co (Hazen) Hydrazine Test μg/l N 2 H Hydrogen Peroxide Test H 2 O Hydrogen Peroxide Cell Test H 2 O 2 H 2 O Hydrogen sulfide Hydroquinone I Iodine color number IFZ Iodine Test I Iron Test 2) Fe Iron Test 2) Fe Iron Cell Test 2) Fe Iron Cell Test 2) Fe Isoascorbic acid (erythorbic acid) L Lead Test 2) Pb Lead Cell Test 2) Pb M Magnesium Cell Test Mg Manganese Test Mn Manganese Test Mn Manganese Cell Test Mn Mercury Hg Methylethylketoxime (2-Butanoneoxime) Molybdenum Cell Test Mo MoO 4 2+ Na 2 MoO Molybdenum Test Mo MoO 4 2+ Na 2 MoO useable in NOVA 30 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 4) Cat. No (Water for on-line analysis) is required for digestion of the sample.

97 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA /Pharo [mg/l] [mg/l] application Alizarinkomplexone analogous EPA 340.3, APHA 4500-F- E for determinations in the low measuring range see manual NOVA / Pharo Alizarinkomplexone analogous EPA 340.3, APHA 4500-F- E for determinations in the low measuring range see manual NOVA / Pharo Alizarinkomplexone analogous EPA 340.3, APHA 4500-F- E / , 13, 15, / / , 13, 15, / , 10, 13, 15, 18 Chromotropic acid , 20, / , 9, 10, 11, 15, 18 Chromotropic acid / , 9, 10, 11, 15, 18 Rhodamine B / , 16, see Total Hardness or Residual Hardness own coloring physical measurement 10, 20, 50 4-(Dimethylamino)-benzaldehyde analogous DIN P , 20, / Neocuproin , / , 7, 9, 11, 12, 13, 14, 15 Titanyl sulfate analogous DIN H15 for determinations in the low measuring range see manual NOVA / , 9, 16, 18 see Sulfide see Oxygen Scavengers Test own coloring analogous to DIN 6162 A 10, 20, 50 DPD 10 10, 20, / , 9, 17 Triazine , 20, / , 2, 5, 6, 9, 13, 15, 16, 17, 18 1,10-Phenanthroline distinction between Fe(II) and Fe(III) possible, analogous APHA 3500-Fe D, DIN E , 20, / , 2, 5, 6, 9, 10, 13, 15, 16, 17, 18 Triazine / , 2, 6, 9, 10, 13, 15, 16, 18 2,2 -Bipyridine distinction between Fe(II) and Fe(III) possible / , 10, 18 see Oxygen Scavengers Test PAR , 20, / , 8, 9, 10, 11, 15, 18 PAR / , 2, 6, 9, 12, 13, 15, 16, 18 Phthaleinkomplexone / , 2, 9, 10, 15, 18 PAN , 20, / , 2, 9, 10, 13, 15 Formaldioxime analogous DIN E , 20, / , 2, 9, 10, 13, 15, 18 Formaldioxime analogous DIN E / , 2, 9, 10, 13, 18 Application see Oxygen Scavengers Test Bromopyrogallol red / , 5, 18 Mercaptoacetic acid / , 11 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 97

98 Index M-N Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests M Monochloramine Test Cl 2 NH 2 Cl NH 2 Cl-N N Nickel Test 2) Ni Nickel Cell Test 2) Ni Nickel in electroplating baths g/l g/l - Ni Nitrate Test 2) NO 3 -N NO Nitrate Test 2) NO 3 -N NO 3 Nitrate Cell Test 2) NO 3 -N NO 3 Nitrate Cell Test 2) NO 3 -N NO Nitrate Cell Test 2) NO 3 -N NO Nitrate Cell Test NO 3 -N NO Nitrate Cell Test in seawater NO 3 -N NO 3 Nitrate Test in seawater NO 3 -N NO Nitrite Test μg/l μg/l NO 2 -N 335 NO Nitrite Cell Test μg/l μg/l NO 2 -N NO 2 Nitrite Cell Test NO 2 -N NO 2 Nitrogen (total) Cell Test 2) N Nitrogen (total) Cell Test 2) N Nitrogen (total) Cell Test 2) 4) N useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 4) Cat. No (Water for on-line analysis) is required for digestion of the sample. 98

99 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA /Pharo [mg/l] [mg/l] application Indophenol blue , 20, / , 9, 17 Dimethylglyoxime analogous APHA 3500-Ni E , 20, / , 5, 8, 9, 10, 11, 13, 15, 18 Dimethylglyoxime analogous APHA 3500-Ni E / , 5, 8, 10, 11, 18 Application , 20, 50 2,6-Dimethylphenol analogous ISO 7890/1, DIN D , 20, / , 6, 8, 9, 11, 13, 15, 17, 18 Nitrospectral , / , 6, 9, 11, 13, 15, 17, 18 Nitrospectral / , 2, 6, 9, 11, 13, 15, 17, 18 2,6-Dimethylphenol analogous ISO 7890/1, DIN D9 2,6-Dimethylphenol analogous ISO 7890/1, DIN D9 2,6-Dimethylphenol analogous ISO 7890/1, DIN D / , 2, 6, 9, 11, 13, 15, 17, / , 8, 11, /- 6 1, 8, 11, 18 Resorcinol / , 2, 8, 11, 13, 16, 18 Resorcinol / , 2, 8, 11, 13, 16, 18 Griess reaction analogous EPA 354.1, APHA 4500-NO2- B, DIN EN D10 Griess reaction analogous EPA 354.1, APHA 4500-NO2- B, DIN EN D , 20, / , 5, 8, 9, 10, 11, 13, 15, 16, / , 5, 8, 9, 10, 11, 13, 15, 16, 18 Iron sulfate / , 10, 13, 16, 18 Koroleff digestion, Koroleff digestion, nitrospectral Koroleff digestion, 2,6-dimethylphenol digestion analogous analogous ISO 7890/1, DIN D9 digestion analogous to DIN EN ISO H36 digestion analogous DIN EN ISO H36, determination analogous ISO 7890/1, DIN D / , 2, 5, 11, 13, 14, / , 2, 5, 11, 13, 14, /- 5 1, 8, 11, 14, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 99

100 Index O-P Spectroquant test kits O Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests Organic Carbon, Total Oxygen Cell Test O Oxygen Demand, Biological Oxygen Demand, Chemical Oxygen Scavengers Test DEHA Carbohy Hydro ISA MEKO Ozone Test O P Palladium Pd Peroxide ph Cell Test 3) ph ph ph ph Phenol Test Phenol Phenol Cell Test Phenol Phosphate Test 2) (ortho-phosphate) PO 4 -P PO 4 P 2 O Phosphate Cell Test 2) (ortho-phosphate) PO 4 -P PO 4 P 2 O Phosphate Cell Test 2) (ortho-phosphate and total phosphorus) USEPA approved 1c) PO 4 -P PO 4 P 2 O Phosphate Cell Test 2) (ortho-phosphate) PO 4 -P PO 4 P 2 O Phosphate Cell Test 2) (ortho-phosphate and total phosphorus) USEPA approved 1c) PO 4 -P PO 4 P 2 O Phosphate Cell Test (ortho-phosphate) PO 4 -P PO 4 P 2 O Phosphate Test (ortho-phosphate) PO 4 -P PO 4 P 2 O Phosphate Test (ortho-phosphate) PO 4 -P PO 4 P 2 O useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 3) The cell test contains three round-bottomed cells each bearing a barcode label. The sample is prepared and measured in the round-bottomed cell. After measurement the round-bottomed cells can be emptied and cleaned for subsequent measurements. The round-bottomed cells can thus be used several times.

101 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml NOVA/Pharo [mg/l] [mg/l] application see TOC 2, 5, 11, 13, 17 mod. Winkler method /- 0.3 see BOD see COD Iron reduction / DPD analogous DIN G , 20, / , 9, 15 Application see Hydrogen peroxide Indicator 10 +/- 0.1 ph 5, 7, 9, 13, 15, 17 4-Aminoantipyrine analogous EPA 420.1, APHA 5530, ISO , 20, / / , 9, 11, 13, 16, 18 MBTH / , 11, 13, 16, 18 Phosphormolybdenum blue analogous EPA , APHA 4500-P E, DIN EN ISO , 20, / , 2, 5, 9, 11, 13, 15, 16, 18 Phosphormolybdenum blue Phosphormolybdenum blue analogous EPA , APHA 4500-P E, DIN EN ISO 6878 analogous EPA , APHA 4500-P E, DIN EN ISO / , 2, 5, 9, 11, 13, 16, / , 2, 5, 9, 11, 13, 16, 18 Phosphormolybdenum blue Phosphormolybdenum blue analogous EPA , APHA 4500-P E, DIN EN ISO 6878 analogous EPA , APHA 4500-P E, DIN EN ISO / , 4, 8, 11, 16, / , 4, 8, 11, 16, 18 Vanadatomolybdate analogous APHA 4500-P C / , 16 Vanadatomolybdate analogous APHA 4500-P C , / , 16 Phosphormolybdenum blue analogous EPA , APHA 4500-P E, DIN EN ISO / , 4, 8, 11, 12, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 101

102 Index P-S Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/40 Pharo 100/300 Multy form of tests P Phosphate Cell Test (ortho-phosphate) PO 4 -P PO 4 P 2 O Phosphate Cell Test (ortho-phosphate and total phosphorus) PO 4 -P PO 4 P 2 O Platinum Pt Platinum-Cobalt Standard Method Potassium Cell Test K Potassium Cell Test K Protein Test g/l g/l - Protein Protein Test g/l g/l - Protein R Residual Hardness Cell Test S SAC (Spectral absorption coefficient) Ca d e f CaO CaCO m m -1 - Silicate (Silicic Acid) Test Silicate (Silicic Acid) Test 4) SiO 2 Si SiO 2 Si Silver Test 2) Ag Sodium Cell Test in nutrient solutions for fertilization Na Sulfate Cell Test SO USEPA approved 1a) Sulfate Test Sulfate Cell Test SO Sulfate Cell Test SO USEPA approved 1a) 102 useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 4) Cat. No (Water for on-line analysis) is required for digestion of the sample.

103 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA/Pharo [mg/l] [mg/l] application Phosphormolybdenum blue Phosphormolybdenum blue analogous EPA , APHA 4500-P E, DIN EN ISO 6878 analogous EPA , APHA 4500-P E, DIN EN ISO / , 4, 8, 11, 16, / , 4, 8, 11, 16, 18 Application see Color Kalignost, turbidimetric / , 12, 13, 15, 16 Kalignost, turbidimetric Bradford Method Biuret Method Phthalein komplexone /- 14 1, 16 Method not programmed in the photometers Method not programmed in the photometers / , 5, 9 physical measurement at 436 nm (Pharo 100) and nm (Pharo 300) see also color measurement Silicomolybdenum blue analogous APHA 4500-Si E, DIN D , 20, / , 6, 9, 13, 16 Molybdosilicate analogous APHA 4500-Si D / / , 6, 9, 13 Eosine, 1,10- phenanthroline reagents for the digestion in the thermoreactor are included in the test kit , / , 18 Iron(III)- thiocyanate determination as chloride / Barium sulfate, turbidimetric analogous EPA 375.4, APHA 4500-SO42- E /- 8 1, 6, 9, 11, 13, 15, 16 Tannic acid /- 15 6, 9, 11, 13, 15 Barium sulfate, turbidimetric Barium sulfate, turbidimetric analogous EPA 375.4, APHA 4500-SO42- E analogous EPA 375.4, APHA 4500-SO42- E /- 17 1, 6, 9, 11, 13, 15, /- 41 1, 4, 6, 8, 9, 11, 13, 15, 16, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 103

104 Index S-Z Spectroquant test kits Measuring range Measuring range Measuring range Parameter [mg/l] [mg/l] [mg/l] Reference Number Ord. No. NOVA 30/60/400 Pharo 100/300 Multy form of tests S Sulfide Test S Sulfite Cell Test SO 3 SO Sulfite Test SO 3 SO Surfactants (anionic) Cell Test MBAS Surfactants (cationic) Cell Test CTAB Surfactants (nonionic) Cell Test Triton X Suspended solids susp. solids T Tin Cell Test Sn TOC Cell Test TOC TOC Cell Test 4b) TOC Screw caps for Spectroquant TOC digestion TOC-Standard 1000 ± 10 mg/l 100 ml Total Alkalinity Total Hardness Cell Test Ca d e f CaO CaCO V Total Nitrogen Transmission % % T Turbidity FAU Volatile Organic Acids acetic acid Cell Test W Water Hardness Z Zinc Cell Test 2) μg/l Zn Zinc Test 2) Zn Zinc Reagent 6 (Isobutylmethylketone GR) Zinc Cell Test 2) Zn useable in NOVA 30 1) This method is officially recognized by the USEPA as an alternative method for the investigation of a) wastewater, b) drinking water, and, respectively, c) drinking water and wastewater. 2) For details of the sample preparation and of the determination of the total content please look into the package inserts in the internet. 4) Cat. No (Water for on-line analysis) is required for digestion of the sample.

105 Method Comments Pipette Cell size [mm] LLD Accuracy Areas of volume [ml] NOVA /Pharo [mg/l] [mg/l] application Dimethyl-pphenylenediamine analogous EPA 376.2, APHA 4500-S2- D, ISO 10530; DIN D , 20, / , 8, 9, 11, 13, 15, 18 Ellman s reagent for determinations of the low measuring range see manual NOVA / Pharo / , 3, 5, 15, 18 Ellman s reagent / , 5, 12, 13, 15, 18 Methylene blue analogous EPA 425.1, APHA 5540 C, DIN EN 903 H / , 11, 13, 18 Disulfine blue analogous DIN H / , 11, 13, 18 TBPE / , 11, 13, 18 physical measurement 16, 20 Pyrocatechol violet / , 10, 16, 18 Indicator / , 11, 13, 18 Indicator /- 43 8, 11, 13, 18 for multiple use, additionally required for TOC measurement analogous EN 1484-H43, DIN H3 Phthalein komplexone see Acid capacity to ph /- 8 2, 9, 13 see Nitrogen (total) 10, 20, Hydroxamic acids / iron(iii) salt /- 75 4, 8, 11, 18 see Total Hardness or Res. Hardness PAR / , 5, 9, 10, 11, 13, 15, 18 Cl-PAN / , 6, 8, 9, 10, 11, 15, 18 Extracting agent for Zinc Test PAR / , 6, 8, 9, 10, 11, 15, 18 Areas of application: 3 Beverages 7 Disinfection control 11 Environment 15 Mineral water 4 Biotechnology, fermenter 8 Disposal drainage water 12 Food 16 Seawater 1 Agriculture 5 Boiler water, cooling water 9 Drinking water 13 Groundwater, surface water 17 Swimming pools 2 Aquaristics 6 Construction-material industry 10 Electroplating surf. refin. 14 Milk dairy products 18 Wastewater 105

106 Spectroquant test kits for testing seawater Simply competent The higher salt content of seawater may interfere with the reagents of test kits developed for drinking and waste water. We have tested our Spectroquant test kits range for the suitability to analyse seawater. Select the test parameter of your choice from the table which informs about the limit of tolerance of the mentioned salts and whether the test kit works in seawater. Overview A-C Suitability of test kits for testing seawater Test kit Ord. No. Seawater Limit of tolerance, salts in % NaCl NaNO 3 Na 2 SO 4 A Acid Capacity Cell Test no Aluminium Cell Test yes Aluminium Test yes Ammonium Cell Test no Ammonium Cell Test yes Ammonium Cell Test yes Ammonium Cell Test yes Ammonium Test yes Ammonium Test yes AOX Cell Test no Arsenic Test no B BOD Cell Test yes Boron Cell Test yes Boron Test no Bromine Test no

107 Overview C-F Suitability of test kits for testing seawater Test kit Ord. No. Seawater Limit of tolerance, salts in % NaCl NaNO 3 Na 2 SO 4 C Cadmium Cell Test no Cadmium Test no Calcium Cell Test no Calcium Test yes Calcium Test no Chloride Cell Test yes 20 1 Chloride Test yes Chlorine Cell Test no Chlorine Cell Test no Chlorine Test no Chlorine Test no Chlorine Test no Chlorine Reagent (liquid) (free and total) no Chlorine dioxide Test no Chromate Cell Test (Chromium VI) yes Chromium (total) Cell Test no Chromate Test yes COD Cell Test no COD Cell Test no COD Cell Test no COD Cell Test no COD Cell Test no COD Cell Test no COD Cell Test no COD Cell Test (Hg free) no COD Cell Test (Hg free) no Copper Cell Test yes Copper Test yes Cyanide Cell Test no Cyanide Test no Cyanuric Acid Test no F Fluoride Cell Test no Fluoride Cell Test no Fluoride Test yes Formaldehyde Cell Test no Formaldehyde Test no

108 Overview G-P Suitability of test kits for testing seawater Test kit Ord. No. Seawater Limit of tolerance, salts in % NaCl NaNO 3 Na 2 SO 4 G Gold Test yes H Hardness, see Total Hardness Cell Test Hydrazine Test no Hydrogenperoxide Cell Test yes Hydrogenperoxide Test no I Iodine Test no Iron Cell Test yes Iron Cell Test no Iron Test yes Iron Test yes L Lead Cell Test no Lead Test no M Magnesium Cell Test yes Manganese Test no Manganese Test no Manganese Test yes Molybdenum Cell Test no Molybdenum Test no Monochloramine Test no N Nickel Cell Test no Nickel Test no Nitrate Cell Test no Nitrate Cell Test no Nitrate Cell Test no Nitrate Cell Test no 2 20 Nitrate Test no Nitrate Test no Nitrate Cell Test (seawater) yes Nitrate Test (seawater) yes Nitrite Cell Test yes Nitrite Cell Test yes Nitrite Test yes Nitrogen (total) Cell Test no Nitrogen (total) Cell Test no Nitrogen (total) Cell Test no 2 20 O Oxygen Cell Test no Oxygen Scavengers Test no Ozone Test no P ph Cell Test yes Phenol Cell Test yes Phenol Test yes

109 Overview P-Z Suitability of test kits for testing seawater Test kit Ord. No. Seawater Limit of tolerance, salts in % NaCl NaNO 3 Na 2 SO 4 P Phosphate (ortho-phosphate) Cell Test yes Phosphate (ortho-phosphate) Cell Test yes Phosphorus (total) Cell Test no Phosphate (ortho-phosphate) Cell Test yes Phosphate (ortho-phosphate) Cell Test yes Phosphorus (total) Cell Test yes Phosphate (ortho-phosphate) Cell Test yes Phosphate (ortho-phosphate) Cell Test yes Phosphorus (total) Cell Test yes Phosphate Test yes Phosphate Test yes Phosphate Cell Test yes Phosphate Test yes Potassium Cell Test yes Potassium Cell Test yes R Residual Hardness Cell Test no S Silicate (Silicic Acid) Test yes Silicate (Silicic Acid) Test no Silver Test no Sodium Cell Test no 10 1 Sulfate Cell Test yes Sulfate Cell Test yes Sulfate Cell Test yes Sulfate Test no Sulfide Test no Sulfite Cell Test no Sulfite Test no Surfactants (anionic) Cell Test no Surfactants (cationic) Cell Test no Surfactants (nonionic) Cell Test no T Tin Cell Test yes TOC Cell Test no TOC Cell Test no Total Hardness Cell Test no V Volatile Organic Acids Cell Test no Z Zinc Cell Test no Zinc Cell Test no Zinc Test no

110 Spectroquant test kits Spectroquant test kits for Hach instruments The following Spectroquant test kits are suitable for use only in conjunction with Hach instruments. They do not require any further device-specific calibration, but instead use the original programmes installed by Hach and work exactly according to the specifications supplied by Hach in the respective instrument operation manual. Thus you don t need to forego Merck s quality documentation when using Hach instruments. The corresponding batch certificates can be downloaded from the internet at Overview A-C Spectroquant test kits for Hach instruments C Parameter Measuring range No. of Merck Method in mg/l tests Ord. No. Chlorine Powder Packs for non-merck photometers Cl DPD for 10-ml-samples (free Chlorine) Chlorine Powder Packs for non-merck photometers for 25-ml-samples (free Chlorine) Chlorine Powder Packs for non-merck photometers for 10-ml-samples (total Chlorine) Chlorine Powder Packs for non-merck photometers for 25-ml-samples (total Chlorine) COD Cell Tests for non-merck photometers USEPA approved Cl DPD Cl DPD Cl DPD COD Oxidation with Chromosulfuric acid, determination as chromate COD Cell Tests for non-merck photometers USEPA approval pending COD Oxidation with Chromosulfuric acid, determination as chromate COD Cell Tests for non-merck photometers USEPA approval pending COD Oxidation with Chromosulfuric acid, determination as chromate COD Cell Tests non-merck photometers USEPA approval pending COD Oxidation with Chromosulfuric acid, determination as chromate 110

111 Hach Comments Pipette- Cell size Areas of Ord. No. volume Hach application analogous EPA 330.5, APHA 4500-Cl G analogous EPA 330.5, APHA 4500-Cl G analogous EPA 330.5, APHA 4500-Cl G analogous EPA 330.5, APHA 4500-Cl G analogous EPA 410.4, APHA 5220 D and ISO analogous EPA 410.4, APHA 5220 D and ISO analogous EPA 410.4, APHA 5220 D and ISO analogous EPA 410.4, APHA 5220 D and ISO ml 1 inch 2, 7, 9, 11, 13, 16, 17, ml 1 inch 2, 7, 9, 11, 13, 16, 17, ml 1 inch 2, 7, 9, 11, 13, 16, 17, ml 1 inch 2, 7, 9, 11, 13, 16, 17, ml 16 mm 5, 9, 10, 11, 13 15, 17, ml 16 mm 5, 9, 10, 11, 13, 15, 17, ml 16 mm 3, 4, 5, 8, 10, 11, 13, ml 16 mm 3, 4, 5, 8, 10, 11, 13, 18 Areas of application: 1 Agriculture 2 Aquaristics 3 Beverages 4 Biotechnology, fermenter 5 Boiler water, cooling water 6 Construction-material industry 7 Disinfection control 8 Disposal drainage water 9 Drinking water 10 Electroplating surf. refin. 11 Environment 12 Food 13 Groundwater, surface water 14 Milk dairy products 15 Mineral water 16 Seawater 17 Swimming pools 18 Wastewater 111

112 Overview I-Z Spectroquant test kits for Hach instruments I Parameter Measuring range No. of Merck Method in mg/l tests Ord. No. Iron Powder Packs for non-merck photometers Fe Phenanthroline for 10-ml-samples M Molybdenum Test Mo MoO Na 2 MoO 4 N Nitrite Powder Packs for non-merck photometers for 25-ml-samples O Oxygen Scavengers Test DEHA Carbohy Hydro ISA MEKO Mercaptoacetic acid NO 2 -N Diazotization Iron Reduction S Sulfate Powder Packs for non-merck photometers for 10-ml-samples Sulfate Powder Packs for non-merck photometers for 25-ml-samples SO Barium chloride SO Barium chloride 112

113 Hach Comments Pipette- Cell size Areas of Ord. No. volume Hach application analogous APHA 3500-Fe D 10 ml 1 inch 1, 2, 5, 7, 8, 9,10, 11, 13, 15, 17, ml 1 inch 5, ml 1 inch 1, 2, 3, 5, 8, 9, 10, 11, 13, 15, ml 1 inch analogous EPA ml 1 inch 1, 5, 6, 7, 8, 9, 10 11, 13, 15, analogous EPA ml 1 inch 1, 5, 6, 7, 8, 9, 10 11, 13, 15, 18 Areas of application: 1 Agriculture 2 Aquaristics 3 Beverages 4 Biotechnology, fermenter 5 Boiler water, cooling water 6 Construction-material industry 7 Disinfection control 8 Disposal drainage water 9 Drinking water 10 Electroplating surf. refin. 11 Environment 12 Food 13 Groundwater, surface water 14 Milk dairy products 15 Mineral water 16 Seawater 17 Swimming pools 18 Wastewater 113

114 Spectroquant supplementary software Analysis methods for the brewery industry Setting the standard for the brewery industry, Merck Millipore offers a comprehensive range of 21 methods for analysis throughout the beer brewing process. The new Spectroquant Pharo software package, Analysis methods for the brewery industry, provides complete beer analysis from raw materials to finished product. The test procedures follow the MEBAK (Mitteleuropäische Brautechnische Analysenkommission) or EBC (European Brewery Convention) methods and are described in the accompanying manual. Users are guided step-by-step through reagent preparation, sample processing and analysis. Merck Millipore has also included information about test solution stability and storage, that can t be found in the standard MEBAK method description, to further support the success of your analysis. 114

115 Your Benefits at a Glance Saves time and reduces errors thanks to pre-calibrated methods. Easy handling - the method descriptions are clear and can be used like a cookery book. Accurate measurements thanks to user-defined calibration when required. Method Sample type Pharo 100 Pharo 300 Acids Anthocyanogenes, Harris and Ricketts method Bitterness - beer (EBC method) Bitterness - wort (EBC method) Iron, spectrophotometric (EBC method) Color, spectrophotometric (EBC method) Flavanoids (EBC method) Free Amino Nitrogen, Ninhydrin method - light beer (EBC method) Free Amino Nitrogen, Ninhydrin method - light wort (EBC method) Free Amino Nitrogen, Ninhydrin method - dark beer (EBC method) Free Amino Nitrogen, Ninhydrin method - dark wort (EBC method) Total Carbohydrates (EBC method) Total Polyphenols (EBC method) Iso- Acids Copper, Cuprethol method (EBC method) Nickel (EBC method) Photometric Iodine Test Reducing Power, spectrophotometric Thiobarbituric Acid Number (TAN) Vicinal Diketones (Diacetyl, 2,3-Pentandione), spectrophotometric (EBC method) Steam-volatile Phenols beer and wort beer and wort beer wort beer beer and wort beer light beer light wort dark beer dark wort beer beer and wort beer and wort beer beer beer and wort beer beer, wort, and congress wort beer beer and malt Spectroquant Supplementary Software Analysis Methods for the Brewery Industry Ord. No Scope of delivery USB stick with the Supplementary Software Analysis Methods for the Brewery Industry, the unlock software, the manuals in English and German. Installation booklet 115

116 Spectroquant quality Simply reliable Quality is a given at Merck. We draw our competence in this field from more than 100 years of experience in the analytical sector. This is also reflected in the official titles of approval awarded to us: Merck has been certified according to DIN EN ISO 9001 ever since 1992 and DIN EN ISO since

117 Quality of the Spectroquant test kits Stringent controls on our raw materials, effective in-process controls and documented final controls are a confirmation for the consistently high quality of our test kits. Every batch that is manufactured receives an individual batch certificate, meaning that you can be released with the effort of checking the quality of the test kit upon its receipt. Our batch certificates, which can be downloaded from the internet at can also be presented for inspection at official audits. The American Environmental Protection Agency has officially approved the suitability of many of our Spectroquant tests and awarded them the quality seal USEPA Approved. Validation of the methods of analysis Merck precisely documents all process-related data obtained in the development laboratory, from measuring ranges and limits of detection all the way to calibration functions. These data are for us key quality characteristics and as such constitute the basis for quality assurance in routine analysis. Certificate for the suitability of Spectroquant test kits for self-monitoring operations The characteristic procedural data, determined according to standardized methods, are given summarically in the form of a quality certificate. This certificate is proof of the consistent quality of all batches that have been delivered to customers so far. Quality certificates as well as batch certificates for each individual Spectroquant test kit can be accessed on the internet at Quality of the Spectroquant photometers The same principles apply to the manufacture of the photometers: each instrument is accompanied by an inspection protocol that confirms not only the quality of the optics systems (photometric accuracy, linearity, reproducibility, wavelength accuracy), but also the electrical safety according to IEC

118 Spectroquant quality assurance Simply complete Start-to-finish quality-assurance measures ensure that your measurement results count as secure and reproducible analytical results. What s more, the Spectroquant NOVA and Pharo photometers support you in ensuring GLP-compliant operations. The components of our professional AQA concept are established media for your Internal Quality Control (IQC) activities, such as those required e.g. in the A704 official memorandum of the Abwassertechnische Vereinigung (German Wastewater Engineering Association). AQA 1 page 121 AQA 3 page 122 AQA 2 page 122 Instrument-supported AQA combines 3 essential parts of quality control to ensure correct results. All target values and tolerances are preprogrammed in the instruments. This makes your AQA checks very easy. AQA 1 Checking the photometer Easy to perform with PhotoCheck and/or CertiPUR UV/VIS standards. AQA 2 Checking the complete system [instrument + test kit + pipette + operator] Reliable documentation by measuring the recovery with CombiCheck, diluted standard solutions (CRM), or CertiPUR standard solutions. AQA 3 Checking the influence of the sample [matrix check] Conveniently guided multiple spiking and dilution procedure supported by the Spectroquant Pharo software. Choose between one time spiking (CombiCheck R-2 solution) or multiple dilution/resp. spiking. (spiking solutions prepared by user) 118

119 AQA 1 checking the photometer In the AQA1 mode the Spectroquant NOVA and Pharo photometers themselves are monitored by means of certified color standards (Spectroquant PhotoCheck) as well as the CertiPUR UV/VIS standards. 1 Spectroquant PhotoCheck Ord. No Spectroquant PhotoCheck is a complete set of highly stable color solutions. A check in a reference spectrophotometer which itself is monitored using primary standards (NIST standards) confirms that this checking medium can be traced back to international standards. Spectroquant PhotoCheck can hence be tracked back to international standards and is thus for test-instrument checking purposes according to DIN EN ISO 9001 resp All results can be subsequently transferred to a printer or a PC for documentation purposes. CertiPUR UV/VIS standards The consistent and correct functioning of your UV/VIS spectrophotometer can be checked using the CertiPUR standards. The CertiPUR solutions can be used to check the following parameters as per Ph. Eur.: Absorption Stray light Wavelength accuracy Operations as per GLP, GMP, USP and DIN 9001 or EN demand these regular controls. Designation Content Ord. No. Useable in Useable in Pharo 100 Pharo 300 UV/VIS Standard 1 UV/VIS Standard 1A UV/VIS Standard 2 UV/VIS Standard 3 UV/VIS Standard 6 Potassium dichromate solution for absorbance according to DAB and Ph. Eur. 2 x 10 ml K 2 Cr 2 O mg/l in H 2 SO N and 6 x 10 ml H 2 SO N Potassium dichromate solution for absorbance at 430 nm according to DAB und Ph. Eur. 2 x 10 ml K 2 Cr 2 O mg/l in H 2 SO N and 6 x 10 ml H 2 SO N Sodium nitrite solution for stray light testing according to DAB and Ph. Eur. 3 x 10 ml NaNO 2 50 g/l in H 2 O Sodium iodide solution for stray light testing according to DAB and Ph. Eur. 3 x 10 ml Nal 10 g/l in H 2 O Holmium oxide solution reference material for wavelength testing according to DAB and Ph. Eur. 3 x 10 ml Ho 2 O 3 40 g/l in HClO 4 (10% v/v)

120 Spectroquant quality assurance AQA 2 checking the complete system In the AQA2 mode the entire system (instrument + test kit + pipette + operator) is exhaustively monitored using certified standards. 2 Spectroquant CombiCheck Product information see page Standard solutions (CRM) for photometric applications Product information see page 126 CertiPUR standard solution Product information see page 127 AQA 3 checking the influence of the sample Besides testing the overall system, it is also necessary to identify any measurement errors due to possible interferences within the sample. Standard addition (see also Spectroquant CombiCheck addition solutions R-2) or dilution can be used to recognize measurement errors due to matrix effects. Any such interferences can be analyzed on the basis of the recovery rates and correspondingly rectified by taking appropriate countermeasures, such as e.g. pretreating the sample accordingly. 3 In the Spectroquant Pharo spectrophotometers, this function is also supported by the instrument itself (AQA-3 mark). Spectroquant CombiCheck Product information see page AQA-1 + AQA-2: The photometer shows when the next AQA check is due. The intervals can be freely selected by time and, in the case of AQA-2, also by the number of measurements. 120

121 AQA password protected control of the complete system By issuing a password (Spectroquant NOVA photometer) or defining hierarchically structured user groups (Spectroquant Pharo spectrophotometers) you yourself can ensure that the AQA intervals of your photometer are observed. You can instruct the instrument to refuse to carry out a measurement in the event that the quality control measurements and intervals are not adhered to. The method in question is released for further measurements only after it has been successfully subjected to the corresponding quality-assurance checks The documentation of the AQA measures in the measurement report is a further enhancement of GLP-compliant operation. Once it has passed the quality-control check, each result is given the suffix AQA in the report. Secure proof that the system is tested. Spectroquant PipeCheck Ord. No In connection with Internal Quality Control measures (IQC) pipettes are required to be tested at regular intervals. This check is usually carried out by weighing the volume of a liquid on a calibrated precision balance. Such a balance is, however, not always readily available. With Spectroquant PipeCheck you can check your pipettes and document the measurement results accordingly, even if you don t have a balance. When e.g. a 5.0-ml pipette is due for checking, 5.0 ml of distilled water is injected into the Spectroquant PipeCheck cell. The measured absorbance must match that measured in the reference cell. 121

122 Spectroquant quality assurance Checking the entire system with Spectroquant CombiCheck The multiparameter standard solutions contained in the various Spectroquant CombiCheck packs are optimally suited for checking the overall system, from the individual test kit and the measurement instrument itself, all the way to your individual working procedures. These standards can also be directly traced back to NIST primary standards. Each CombiCheck pack contains one standard solution and one addition solution. If the check shows that the specified concentration of the standard solution is reached, the entire analysis system is in order. If, however, deviations from the specified values become apparent, the causes of these deviations must be tracked down. With the aid of the addition solution it is possible to identify measurement errors that are caused by the sample matrix (for example the presence of interfering substances). If the recovery rate is found to be insufficient (beyond the specified tolerances) the reason for the error must be analyzed and eliminated by taking the appropriate countermeasures, such as e.g. pretreating the sample correspondingly. Ord. No Spectroquant CombiCheck 10 to control quality of photometric methods of below listed parameter Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks Standard Solution Reagent R-1 Ammonium 4.00 ± 0.30 mg/l NH 4 -N Chloride 25 ± 6 mg/l Cl COD Nitrate Phosphate 4) Sulfate ± 12 mg/l COD ± 12 mg/l COD ± 0.25 mg/l NO 3 -N ± 0.25 mg/l NO 3 -N ± 0.08 mg/l PO 4 -P ± 0.08 mg/l PO 4 -P ± 0.08 mg/l PO 4 -P ± 0.08 mg/l PO 4 -P ± 15 mg/l SO 4 ± 15 mg/l SO 4 ± 15 mg/l SO ) / ) ) / ) ) Addition Solution Reagent R-2 (for spiking of samples) Ammonium 3.00 ± 0.25 mg/l NH 4 -N Chloride 25 ± 6 mg/l Cl COD Nitrate Phosphate 4) Sulfate ± 8 mg/l COD ± 8 mg/l COD ± 0.20 mg/l NO 3 -N ± 0.40 mg/l NO 3 -N ± 0.07 mg/l PO 4 -P ± 0.07 mg/l PO 4 -P ± 0.07 mg/l PO 4 -P ± 0.05 mg/l PO 4 -P ± 5 mg/l SO 4 ± 15 mg/l SO 4 ± 10 mg/l SO ) ) / ) / ) )

123 Spectroquant CombiCheck 20 to control quality of photometric methods of below listed parameter Ord. No Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks Ammonium 12.0 ± 1.0 mg/l NH 4 -N Standard Solution Reagent R-1 Chloride 60 ± 10 mg/l Cl COD Nitrate Phosphate 4) ± 75 mg/l COD ± 75 mg/l COD ± 0.9 mg/l NO 3 -N ± 0.9 mg/l NO 3 -N ± 0.9 mg/l NO 3 -N ± 0.9 mg/l NO 3 -N ± 0.9 mg/l NO 3 -N ± 0.7 mg/l PO 4 -P ± 0.7 mg/l PO 4 -P / ) ) ) Sulfate 500 ± 75 mg/l SO Ammonium 8.0 ± 0.8 mg/l NH 4 -N Addition Solution Reagent R-2 Chloride 40 ± 7 mg/l Cl (for spiking of samples) COD Nitrate Phosphate 4) ± 40 mg/l COD ± 40 mg/l COD ± 0.8 mg/l NO 3 -N ± 0.6 mg/l NO 3 -N ± 1.5 mg/l NO 3 -N ± 0.6 mg/l NO 3 -N ± 0.8 mg/l NO 3 -N ± 0.5 mg/l PO 4 -P ± 0.5 mg/l PO 4 -P / ) ) ) Sulfate 150 ± 30 mg/l SO Spectroquant CombiCheck 30 to control quality of photometric methods of below listed parameter Ord. No Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks Cadmium ± mg/l Cd Standard Solution Reagent R-1 Copper Iron Manganese ± 0.20 mg/l Cu ± 0.20 mg/l Cu ± 0.15 mg/l Fe ± 0.15 mg/l Fe ± 0.15 mg/l Fe ± 0.15 mg/l Fe ± 0.15 mg/l Mn ± 0.15 mg/l Mn ) / ) ) / ) Cadmium ± mg/l Cd Addition Solution Reagent R-2 Copper 3.00 ± 0.30 mg/l Cu (for spiking of samples) 3.00 ± 0.30 mg/l Cu ) Iron Manganese ± 0.30 mg/l Fe ± 0.30 mg/l Fe ± 0.20 mg/l Fe ± 0.15 mg/l Mn ± 0.15 mg/l Mn / ) ) / ) ) using a 10 mm rectangular cell, Ord.No ) using a 20 mm rectangular cell, Ord.No ) using a 50 mm rectangular cell, Ord.No ) only the determination of ortho-phosphate can be checked 123

124 Spectroquant quality assurance Ord. No Spectroquant CombiCheck 40 to control quality of photometric methods of below listed parameter Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks Standard Solution Reagent R-1 Aluminium 0.75 ± 0.08 mg/l Al ) Lead Nickel ± 0.20 mg/l Pb ± 0.20 mg/l Pb ± 0.20 mg/l Ni ± 0.20 mg/l Ni ) ) 5.0 Zinc 2.00 ± 0.40 mg/l Zn Addition Solution Reagent R-2 Aluminium 1.00 ± 0.10 mg/l Al ) (for spiking of samples) Lead Nickel ± 0.15 mg/l Pb ± 0.10 mg/l Pb ± 0.20 mg/l Ni ± 0.20 mg/l Ni ) ) 0.1 Zinc 2.00 ± 0.40 mg/l Zn Ord. No Standard Solution Reagent R-1 Ammonium COD Spectroquant CombiCheck 50 to control quality of photometric methods of below listed parameter Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks ± 0.10 mg/l NH 4 -N ± 0.10 mg/l NH 4 -N ± 4.0 mg/l COD ± 4.0 mg/l COD ± 4.0 mg/l COD / ) Nitrogen ± 0.7 mg/l N ± 0.7 mg/l N Addition Solution Reagent R-2 (for spiking of samples) Ammonium COD ± 0.10 mg/l NH 4 -N ± 0.10 mg/l NH 4 -N ± 3.0 mg/l COD ± 3.0 mg/l COD ± 3.0 mg/l COD / ) Nitrogen ± 0.5 mg/l N ± 0.5 mg/l N

125 Spectroquant CombiCheck 60 to control quality of photometric methods of below listed parameter Ord. No Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks Chloride 125 ± 13 mg/l Cl / ) Standard Solution Reagent R-1 COD ± 25 mg/l COD ± 20 mg/l COD Chloride 50 ± 7 mg/l Cl / ) Addition Solution Reagent R-2 COD ± 15 mg/l COD ± 10 mg/l COD (for spiking of samples) Spectroquant CombiCheck 70 to control quality of photometric methods of below listed parameter Ord. No Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks Ammonium 50.0 ± 5.0 mg/l NH 4 -N Standard Solution Reagent R-1 Ammonium 50.0 ± 5.0 mg/l NH 4 -N ) ( mg/l) Ammonium (5-150 mg/l) 50 ± 5 mg/l NH 4 -N ) COD ± 400 mg/l COD ± 400 mg/l COD Nitrogen 50 ± 7 mg/l N Ammonium 20.0 ± 2.0 mg/l NH 4 -N Addition Solution Reagent R-2 Ammonium ( mg/l) 10.0 ± 1.0 mg/l NH 4 -N ) (for spiking of samples) Ammonium (5-150 mg/l) 20 ± 2 mg/l NH 4 -N ) COD 2000 ± 200 mg/l COD Nitrogen 20 ± 6 mg/l N Spectroquant CombiCheck 80 to control quality of photometric methods of below listed parameter Ord. No Parameter Concentration and working tolerance can be used for test kits Ord.No. Standard solution (ml) No. of quality checks COD 1500 ± 150 mg/l COD Standard Solution Reagent R-1 Nitrate 25.0 ± 2.5 mg/l NO 3 -N Phosphate 4) ± 1.0 mg/l PO 4 -P ± 1.0 mg/l PO 4 -P COD 1000 ± 100 mg/l COD Addition Solution Reagent R-2 Nitrate 10.0 ± 1.5 mg/l NO 3 -N (for spiking of samples) Phosphate 4) ± 0.5 mg/l PO 4 -P ± 0.5 mg/l PO 4 -P ) using a 10 mm rectangular cell, Ord.No ) using a 20 mm rectangular cell, Ord.No ) using a 50 mm rectangular cell, Ord.No ) only the determination of ortho-phosphate can be checked 125

126 Spectroquant quality assurance Analytical quality control Standard solutions (CRM) for photometric applications By using the certifed, diluted standard solutions for photometric applications you can verify your measurement results without extra effort. These standard solutions are traceable to SRM from NIST and are completely ready-to-use. Time-consuming dilution is no longer necessary which eliminates possible sources of error. The lot certifcates document all batch-specifc and analysis-relevant data, such as exact concentration and expanded measurement uncertainty. Most importantly, it provides you with the certitude that the diluted, certifed standard solutions are traceable directly to primary standard reference materials from NIST. This ensures that quality standards are comparable internationally.! The lot certifcate for each standard solution can be accessed easily online at any time: Ordering Information for Standard solutions, (CRM), 100 ml in H 2 O Product Concentration Expanded Ord. No. Measurement uncertainty A Ammonium standard solution mg/l NH 4 -N ± mg/l Ammonium standard solution 1.00 mg/l NH 4 -N ± 0.04 mg/l Ammonium standard solution 2.00 mg/l NH 4 -N ± 0.07 mg/l Ammonium standard solution 6.00 mg/l NH 4 -N ± 0.13 mg/l Ammonium standard solution mg/l NH 4 -N ± 0.4 mg/l Ammonium standard solution 50.0 mg/l NH 4 -N ± 1.2 mg/l C COD standard solution 20.0 mg/l ± 0.7 mg/l COD standard solution 100 mg/l ± 3 mg/l COD standard solution 200 mg/l ± 4 mg/l COD standard solution 400 mg/l ± 5 mg/l COD standard solution 1,000 mg/l ± 11 mg/l COD standard solution 2,000 mg/l ± 32 mg/l COD standard solution 8,000 mg/l ± 68 mg/l COD standard solution 50,000 mg/l ± 894 mg/l N Nitrate standard solution 0.50 mg/l NO 3 -N ± 0.05 mg/l Nitrate standard solution 2.50 mg/l NO 3 -N ± 0.06 mg/l Nitrate standard solution 15.0 mg/l NO 3 -N ± 0.4 mg/l Nitrate standard solution 40.0 mg/l NO 3 -N ± 1 mg/l Nitrate standard solution 200 mg/l NO 3 -N ± 5 mg/l Nitrite standard solution mg/l NO 2 -N ± mg/l Nitrite standard solution 40.0 mg/l NO 2 -N ± 1.3 mg/l Nitrogen (total) standard solution 2.50 mg/l N ± 0.06 mg/l Nitrogen (total) standard solution 12.0 mg/l N ± 0.3 mg/l Nitrogen (total) standard solution 100 mg/l N ± 3 mg/l P Phosphorus (total) standard solution mg/l PO 4 -P ± mg/l Phosphorus (total) standard solution 4.00 mg/l PO 4 -P ± 0.08 mg/l Phosphorus (total) standard solution 15.0 mg/l PO 4 -P ± 0.4 mg/l S Sulfate standard solution 40 mg/l SO 4 ± 6 mg/l Sulfate standard solution 125 mg/l SO 4 ± 6 mg/l Sulfate standard solution 400 mg/l SO 4 ± 20 mg/l Sulfate standard solution 800 mg/l SO 4 ± 27 mg/l

127 Spectroquant quality assurance Analytical quality control CertiPUR standard solutions Our comprehensive range of ready-to-use standard solutions is naturally also at your disposal. You can prepare standards for almost every parameter in any concentration you wish simply by making the approriate dilution. Ready to use standard solutions Parameter Concentration in mg/l Volume in mg/l for Ord. No. A Aluminium* Ammonium* Antimony* Arsenic* B Boron* C Cadmium* Calcium* Chloride* Chromate* Chromium* Cobalt* COD reference solution Copper* Cyanide* F Fluoride G Gold* I Iron* L Lead* M Magnesium* Manganese* Mercury* Molybdenum* N Nickel* Nitrate* Nitrite* P Palladium* Phosphate* Platinum* Potassium* S Silver* Silicon* Sulfate* T Tin* TOC* V Vanadium* Z Zinc* With the above standard solutions, you can make standards of different concentrations to meet your needs by dilution. You can also use the above for checking matrix effects by substituting them for additional solution. The stability of the unopened standards are guaranteed for two years, which will provide an economic and easy to handle product for quality control. * traceable to NIST 127

128 Spectroquant quality assurance Analytical quality control - Overview The following tables show you at a glance which quality-assurance product such as e.g. Spectroquant CombiCheck or standard solutions can be used in conjunction with which test kit. In cases where a parameter is not stable for instance chlorine then we naturally provide assistance to the users of our products and offer an application by which such a standard can be prepared. These applications can be found in the preface to our photometer and colorimeter manuals and also in the internet at This ensures that every user is given the optimum support to achieve good results. Overview A-B Test kit Ord. No. Ord. No. Ord. No. Diluted Ready-to-use Alternative Ord. No. Test kit CombiCheck standard solution, CRM Standard Standard A Acid Capacity Cell Test to ph 4.3 (total alkalinity) ) Aluminium Cell Test ) Aluminium Test ) Ammonium Cell Test ) Ammonium Cell Test Ammonium Cell Test Ammonium Cell Test ) ) ) Ammonium Test ) Ammonium Test ) AOX Cell Test mg/l AOX Arsenic Test ) B BOD Cell Test EN 1899, 210mg/l Boron Cell Test ) Boron Test ) Bromate 2) Bromine Test DIN EN ISO ) 1) Standard solution, ready-to-use, 1000mg/l analyt. Traceable to SRM of NIST (see Ord. No. Standards) 2) Own standards. Worksheets how to prepare these standards can be downloaded from our website Quick Links: Analytical Application Finder - Photometry - Sample = Standard 128

129 Overview C Spectroquant quality assurance Test kit Ord. No. Ord. No. Ord. No. Diluted Ready-to-use Alternative Ord. No. Test kit CombiCheck standard solution, CRM Standard Standard C Cadmium Cell Test ) Cadmium Test Calcium Cell Test ) Calcium Test ) Calcium Test ) Chloride Cell Test ) Chloride Test ) Chlorine Cell test (free) DIN EN ISO ) Chlorine Test (free) DIN EN ISO ) Chlorine Test (total) DIN EN ISO ) Chlorine Cell Test (free and total) DIN EN ISO ) Chlorine Test (free and total) DIN EN ISO ) Chlorine Powder Packs 3) (free) Chlorine Powder Packs 3) (total) DIN EN ISO ) DIN EN ISO ) Chlorine Dioxide Test DIN EN ISO ) Chromate Cell Test ) Chromate Test ) COD Cell Test ) COD Cell Test ) COD Cell Test ) COD Cell Test ) COD Cell Test ) COD Cell Test COD Cell Test COD Cell Test COD Cell Test COD Cell Test (Hg-free) COD Cell Test (Hg-free) ) 2) 2) 2) 2) 2) 129

130 Overview C-N Spectroquant quality assurance Test kit Ord. No. Ord. No. Ord. No. Diluted Ready-to-use Alternative Ord. No. Test kit CombiCheck standard solution, CRM Standard Standard COD Cell Test 3) ) COD Cell Test 3) ) COD Cell Test 3) ) COD Cell Test 3) ) Copper Cell Test ) Copper Test Cyanide Test ) Cyanuric Acid Test ) F Fluoride Cell Test ) Fluoride Cell Test ) Fluoride Test ) Formaldehyde Cell Test ) Formaldehyde Test ) G Gold Test ) H Hydrazine Test ) Hydrogen Peroxide Cell Test ) Hydrogen Peroxide Test ) I Iodine Test DIN EN ISO ) Iron Cell Test ) Iron Cell Test ) Iron Test ) Iron Test ) Iron Powder Packs 3) ) L Lead Cell Test ) Lead Test ) M Magnesium Cell Test ) Manganese Cell Test ) Manganese Test ) Manganese Test ) Molybdenum Cell Test ) Molybdenum Test ) Monochloramine Test ) N Nickel Cell Test ) Nickel Test ) Nitrate Cell Test ) Nitrate Cell Test ) Nitrate Cell Test Nitrate Cell Test ) ) ) Standard solution, ready-to-use, 1000mg/l analyt. Traceable to SRM of NIST (see Ord. No. Standards) 2) Own standards. Worksheets how to prepare these standards can be downloaded from our website Quick Links: Analytical Application Finder - Photometry - Sample = Standard 3) for non-merck photometers

131 Overview N-P Spectroquant quality assurance Test kit Ord. No. Ord. No. Ord. No. Diluted Ready-to-use Alternative Ord. No. Test kit CombiCheck standard solution, CRM Standard Standard N Nitrate Test ) Nitrate Test ) Nitrate Cell Test in seawater Nitrate Test in seawater ) ) Nitrite Cell Test ) Nitrite Test ) Nitrite Cell Test ) Nitrite Powder Packs 3) ) Nitrogen (total) Cell Test Nitrogen (total) Cell Test Nitrogen (total) Cell Test O Oxygen Cell Test ) Oxygen Scavengers Test ) Ozone-Test DIN EN ISO ) P ph Cell Test Buffer solution ph Phenol Cell Test ) Phenol Test ) Phosphate (ortho-phosphate) Cell Test ) Phosphate (ortho-phosphate) Cell Test ) Phosphorus (total) Cell Test Phosphate (ortho-phosphate) Cell Test Phosphate (ortho-phosphate) Cell Test 2) 2) 2) 1) ) ) Phosphorus (total) Cell Test Phosphate (ortho-phosphate) Cell Test Phosphate (ortho-phosphate) Cell Test 1) ) ) Phosphorus (total) Cell Test ) Phosphate (ortho-phosphate) ) Cell Test 131

132 Overview P-Z Spectroquant quality assurance Test kit Ord. No. Ord. No. Ord. No. Diluted Ready-to-use Alternative Ord. No. Test kit CombiCheck standard solution, CRM Standard Standard P Phosphate Test (orthwo-phosphate) ) Phosphate Test (ortho-phosphate) ) Phosphate Test (ortho-phosphate) ) Potassium Cell Test ) Potassium Cell Test ) R Residual Hardness Cell Test ) S Silicate (silicic acid) Test ) Silicate (silicic acid) Test ) S Silver Test ) Sodium Cell Test ) Sulfate Cell Test Sulfate Cell Test Sulfate Cell Test ) ) ) Sulfate Test Sulfate Powder Packs 3) ) ) Sulfide Test ) Sulfite Cell Test ) Sulfite Test ) Surfactants (anionic) Cell Test ) Surfactants (cationic) Cell Test ) Surfactants (nonionic) Cell Test ) T Tin Cell Test ) TOC Cell Test ) TOC Cell Test ) Total Hardness Cell Test ) Total Nitrogen Cell Test ) Total Nitrogen Cell Test ) V Total Nitrogen Cell Test ) Volatile Organic ) Acid Cell Test Z Zinc Cell Test ) Zinc Cell Test ) Zinc Test ) ) Standard solution, ready-to-use, 1000mg/l analyt. Traceable to SRM of NIST (see Ord. No. Standards) 2) Own standards. Worksheets how to prepare these standards can be downloaded from our website Quick Links: Analytical Application Finder - Photometry - Sample = Standard 3) For non-merck photometers Cleaning validation The Spectroquant non ionic surfactants cell test (page 102) supports an effective cleaning validation. Details on search Extran.

133 Cleaning agents for cells and glass vessels The cleanliness of the working environment and accessories is an unconditional prerequisite for the accuracy of the measurements. All instruments used must be clean and dry. After the analysis has been completed, the volumetric flasks, pipettes, reagent vessels, and cells used must be thoroughly washed with water immediately and subsequently rinsed with distilled water. The cell surfaces must be absolutely clean, dry, and clear. Any stains or marks adhering to surfaces can be wiped off using a dry cloth. Greasy marks can be removed by immersing the vessel in question in 2-5 % Extran and subsequently rinsing with distilled water. Extran MA 02 neutral is adequate for normal stains and marks. Caution! Do not use in connection with the determination of phosphorus, since Extran MA 02 neutral contains phosphates. The ph of a 2-5 % solution is approx In the case of stubborn stains it is advisable to use Extran MA 03 phosphate-free. Caution! Do not use for alkali-sensitive material such as aluminium etc. The ph of a 2-5 % solution is approx Cleaning agents for cells and glass vessels Ord. No. Extran detergent, MA 02 neutral (1 pack = 2.5 l) Extran detergent, MA 03 phosphate-free (1 pack = 2.5 l)

134 I carry a lot of water - and responsibility! Turbiquant lightens the load all round. Jim G., ultra-pure water supplier Jim s customers demand the highest standards of purity. Luckily, so do his colleagues back at the bottling plant. To make sure the water they supply is always up to par, they use Turbiquant instruments. For fast, reliable turbidity analyses right there on site. 134

135 Turbidimetry Turbiquant General information 136 Turbiquant 1100 IR and 1100 T 140 Turbiquant 1500 IR and 1500 T 141 Turbiquant 3000 IR and 3000 T 142 Turbiquant accessoires 143 Turbiquant calibration standards

136 Turbiquant turbidimetry Fast, simple, exact Measure turbidity with the Turbiquant instruments from Merck. Here you ll find the right solutions for: Drinking water Wastewater Surface water Industrial process water Our instruments are suited for both investigations in the laboratory as well as on-the-spot analyses. We have developed the various models of the Turbiquant turbidimeters with a focus on routine measurements. A feature they all have in common is their ease of use and ready to use, non toxic standards for easy calibration. The Turbiquant handheld instruments: Portable, light weight and battery-operated for on-spot measurements. 136

137 The advantages of the Turbiquant system Easy to operate and calibrate Reliable and reproducible results Measurements according to EN ISO 7027 resp. USEPA GLP functions, data transfer Broad range of standards for immediate use Comprehensive range of accessories, also for continuous-flow measurements Deionized water 0.02 NTU Drinking water 0.02 to 0.5 NTU Turbidity an expressive parameter in drinking- and wastewater treatment, the beverages industry, and in the chemical sector. Here a few typical turbidity values. Spring water 0.05 to 10 NTU Waste water (untreated) 70 to 2,000 NTU Sift water (paper industry) 60 to 800 NTU 137

138 Turbiquant turbidimetry Turbiquant 1100 IR and 1100 T These instruments are particularly suited for on-the-spot measurements. A robust carry-case and the waterproof casing offer additional protection. Turbiquant 1500 IR and 1500 T The instrument automatically selects the correct measuring range. A monitoring feature for the calibration interval makes its operation even easier. The optimal continuous flow cell makes it possible to run pressureless continuous-flow measurements. Turbiquant 3000 IR and 3000 T With its high-precision operation in the measuring range from to 10,000 NTU, this instrument is suited for the analysis of extra-pure water and quality-assurance operations in the beverages industry all the way to the investigation of wastewaters. Comprehensive AQA functions, the display of results in NTU, EBC, FNU, FAU, and nephelos, and the ratio-measurement option satisfies even the most demanding user. In addition to pressureless continuous-flow measurements it is also capable of working continuously up to a pressure of 4 bar. IR or T where s the difference? For standard-compliant measurements according to EN ISO 7027 an IR-LED (infrared) light source with a wavelength of 860 nm is prescribed (IR-models). The USEPA Method and also APHA - AWWA - WPCF require the use of a tungsten lamp emitting white light (T-models). IR measurements show no interference when colored solutions are used. Tungsten lamps are more sensitive in cases in which the most minute particles are to be measured. 138

139 Turbiquant system at a glance Turbiquant 1100 for on-the-spot measurements Instrument Light source Measuring range Applications Special features Turbiquant 1100 IR IR LED ,100 NTU/FNU Portable instrument for on-the-spot analysis Portable, battery-operated instrument Turbiquant 1100 T Tungsten lamp 0,01-1,100 NTU/FNU Portable instrument for on-the-spot analysis Portable, battery-operated instrument Turbiquant 1500 instrument for routine measurements in the laboratory Instrument Light source Measuring range Applications Special features Turbiquant 1500 IR IR LED 0-1,000 NTU/FNU Standard instrument for all applications in the laboratory suitable for drinking water Continuous-flow measurements (pressureless), AQA functions Turbiquant 1500 T Tungsten lamp 0-1,000 NTU/FNU Standard instrument for all applications in the laboratory suitable for drinking water Continuous-flow measurements (pressureless), AQA functions Turbiquant 3000 the precision instrument for turbidimetry Instrument Light source Measuring range Applications Special features Turbiquant IR LED 0-10,000 NTU/FNU/FAU, Precision instrument Continuous-flow measurements 3000 IR 0-2,450 EBC also for demanding (pressureless or up to 4 bar), turbidimetric applications AQA functions, passwort protection, ratio measurements, transmission (IR only) Turbiquant Tungsten lamp 0-10,000 NTU, Precision instrument Continuous-flow measurements 3000 T 0-2,450 EBC, also for demanding (pressureless or up to 4 bar), 0-67,000 NEPHELO turbidimetric applications AQA functions, passwort protection, ratio measurements The measurement method of the Turbiquant 1100/1500/3000 IR instruments conforms with EN ISO 7027, that in the Turbiquant 1100/1500/3000 instruments is analogous to the guidelines of the USEPA. 139

140 Turbiquant instruments Turbidimetry with Turbiquant The Turbiquant 1100 IR and 1100 T instruments are portable, battery-operated turbidimeters. The four standard batteries provide power for more than 5,000 measure ments. The easy operation, the practical carrying case, and the waterproof casing that ensures that the electronic components are adequately protected make the Turbiquant 1100 IR and 1100 T the ideal instruments for on-the-spot analysis. Ord. No Turbiquant Turbidimeter 1100 IR Portable, battery-operated instrument for on-the-spot analysis Scope of delivery 2 empty cells, manual, handy hints, carrying case, 4 batteries Measuring principle nephelometric 90 scattered light, conform with EN ISO 7027 Light source IR LED Indication of units NTU / FNU Measuring range ,100 NTU Resolution 0.01 within the range 0.01 < x < NTU 0.1 within the range < x < NTU 1 within the range 1,000 < x < 1,100 NTU Accuracy ±2 % of reading or ±0.1 NTU for range NTU ±3 % of reading for range 500-1,100 NTU Calibration automatic 3 points or partial (user-defined) Response time 14 seconds Cuvettes 25 x 45 mm Sample volume 15 ml Protection type designed to meet IP 67 Power requirements 4 alkali manganese batteries, AAA / Micro Test certificates CE Warranty 2 years Also required: Turbiquant Calibration Standard Set Ord. No Ord. No Turbiquant Turbidimeter 1100 T Portable, battery-operated instrument for on-the-spot analysis Scope of delivery 2 empty cells, manual, handy hints, carrying case, 4 batteries Specifications same as Turbiquant 1100 IR, but tungsten lamp Measurement principle nephelometric 90 scattered light, follows USEPA recommendations Light source white light tungsten lamp Measurement units NTU / FNU Warranty 2 years Also required: Turbiquant Calibration Standard Set Ord. No IQ and OQ documents for all Turbiquant Instruments further details see page

141 Turbiquant 1500 IR and 1500 T These instruments have large and easy-to-read displays and can be simply operated with just a few buttons. It goes without saying that the user is guided through the operation procedure via the display. A continuous-flow cell enables a high sample throughput to be achieved. Turbiquant Turbidimeter 1500 IR Ord. No For routine measurements in the laboratory Scope of delivery universal power supply / plug 3 empty cells, manual, handy hints Measurement modes nephelometric (non ratio), conform with EN ISO 7027 Light source IR LED Indication of units NTU (= FTU = FNU) Measuring range 0-1,000 NTU Resolution max within the range 0 < x <10 NTU max. 0.1 within the range 10 < x < 100 NTU max. 1 within the range 100 < x < 1,000 NTU Accuracy ±2 % of reading or ±0.01 NTU for range ,000 NTU Reproducibility < ±1% of reading or ±0.01 NTU Calibration automatic 1 to 3 points Response time < 3 seconds Cuvettes 28 x 70 mm Sample volume 25 ml Serial input / output RS 232, uni-directional Real time clock integrated Other functions GLP function (calibration intervals monitoring), automatic self check, integrated real time clock Power requirements universal power supply / plug Test certificates CE, CETPlus Warranty 2 years Also required: Turbiquant Calibration Standard Set Ord. No Turbiquant Turbidimeter 1500 T Ord. No For routine measurements in the laboratory Scope of delivery universal power supply / plug 3 empty cells, manual, handy hints Specifications same as Turbiquant 1500 IR, but tungsten lamp Measurement principle nephelometric (non-ratio), follows USEPA recommendations Light source tungsten lamp Warranty 2 years Also required: Turbiquant Calibration Standard Set Ord. No

142 Turbiquant instruments Turbiquant 3000 IR oder 3000 T In addition to the specifications of the Turbiquant 1500 IR and 1500 T the user can select different measurement methods and result terms. The Turbiquant 3000 IR, for example can display the following units: NTU nephelometric turbidity units (90 diffused-light measurement) EBC guidelines of the European Brewery Convention FNU formazine nephelometric units (calibration with formazine) FAU formazine attenuation units (DIN measurement beyond 40 FNU) The methods available are those of nephelometry, densitometry and ratio measurement. It is also possible to make continuous-flow measurements with a low-pressure continuous-flow cell using a purging gas. Ord. No Turbiquant Turbidimeter 3000 IR The precision instrument Scope of delivery universal power supply / plug, 3 empty cells, manual, handy hints Measurement modes nephelometric (non-ratio / ratio selectable), conform with EN ISO 7027 Light source IR LED Indication of units NTU, FNU, FAU, EBC Measuring range 0-10,000 NTU, 0-10,000 FNU, 0-10,000 FAU, 0-2,450 EBC Resolution selectable NTU max max max max. 0.1 within the range within the range within the range within the range 0 < x <10 NTU 10 < x < 100 NTU 100 < x < 1,000 NTU 1,000 < x < 10,000 NTU Accuracy ±2 % of reading or ±0.01 NTU for range ,000 NTU ±5 % of reading for range 1,000-4,000 NTU ±10 % of reading for range 4,000-10,000 NTU Repeatability < ±1 % of reading or ±0.01 NTU Calibration automatic 1 to 4 points (to 1,750 NTU) 10,000 NTU selectable Response time < 6 s Cuvettes 28 x 70 mm Sample volume 25 ml Serial input / output RS 232, bi-directional Real time clock integrated Other functions GLP function (calibration interval monitoring), automatic self check, integrated real time clock, safety access codes for calibration and instrument setup Power requirements universal power supply / plug Test certificates CE, CETPlus Warranty 2 years Also required: Turbiquant Calibration Standard Set Ord. No

143 Turbiquant Turbidimeter 3000 T Ord. No The precision instrument Scope of delivery Specification Measuring mode Light source Indication of units Measuring range Warranty universal power supply / plug 3 empty cells, manual, handy hints same as Turbiquant 3000 IR, but tungsten halogen lamp nephelometric (non-ratio/ratio selectable), follows USEPA recommendations tungsten lamp NTU, EBC, nephelos 0-10,000 NTU, 0-2,450 EBC, 0-67,000 nephelos 2 years Also required: Turbiquant Calibration Standard Set Ord. No Turbiquant accessoires Empty cells Ord. No. Turbiquant 1000/1100 cells (1 pack = 3 pcs) Turbiquant 1500/3000 cells (1 pack = 3 pcs) Lamps Ord. No. Turbiquant 1500 IR lamp module Turbiquant 3000 IR lamp module Turbiquant 1500/3000 tungsten lamp module Other accessories and cables Ord. No. Turbiquant 1500/3000 cell rack Turbiquant 1500/3000 continuous-flow cell Turbiquant 3000 continuous-flow cell (low-pressure) Printer cabel for Turbiquant 1500/3000 (for serial interface) PC cable for Turbiquant 1500/3000 (for serial interface)

144 Turbiquant instruments Primary calibration standards AMCO-AEPA-1 Microspheres are primary calibration standards that conform with USEPA Method (see also Standard Methods for Examination of Water and Wastewater 21st Edition (2005), Section 2130, Turbidity). In compliance with EN IS0 standard 7027: 1999 Water Quality Turbidimetry these standards count as alternative secondary standards to freshly prepared formazine suspensions. The advantages of the Turbiquant calibration standards 1. Cited as primary calibration standards in compliance with USEPA method Accepted as alternative use secondary standards according EN ISO 7027: More stable than formazin with a minimum shelf life of 12 months. 4. Non toxic. 5. Can be stored and transported without any precautionary measures. 6. Supplied ready-to-use. 7. Coming with indexing ring for quick and repeatable indexing as recommended by US EPA. 8. Incomparable precision: 10 NTU ±1 % 100 NTU ±1 % 1,000 NTU ±1 % 1,750 NTU ±2 % 10,000 NTU ±2 % 144

145 Turbiquant Calibration Standard Sets Turbiquant 1000 Calibration Standard Ord. No. Turbiquant 1000 IR Calibration Standard Set 4 standards ,000 NTU Turbiquant 1100 Calibration Standard Ord. No. Turbiquant 1100 IR / 1100 T Calibration Standard Set 3 standards ,000 NTU Turbiquant 1500 Calibration Standard Ord. No. Turbiquant 1500 IR / 1500 T Calibration Standard Set 3 standards ,000 NTU Turbiquant 3000 Calibration Standard Ord. No. Turbiquant 3000 IR Calibration Standard Set 4 standards ,750 NTU Turbiquant 3000 T Calibration Standard Set 4 standards ,750 NTU Turbiquant 3000 IR Calibration Standard 10,000 NTU Turbiquant 3000 T Calibration Standard 10,000 NTU Turbiquant 1500 / 3000 Calibration Standard 10 NTU Turbiquant Don t forget to add the Turbiquant Calibration Standard Set to your order of the instrument! 145

146 My customers want facts, not opinions. Winston J., Quality Manager Variety is the spice of life but not when it comes to lab findings. So for proven, verifiable results every time, Winston and his team trust their Spectroquant, Reflectoquant and Turbiquant systems backed by our documented quality assurance system. 146

147 Quality Assurance General information 148 Installation Qualification 148 Operational Qualification 149 Performance Qualification

148 Quality Assurance proven and verifiable results Quality comes naturally to us at Merck Millipore. We subject each and every one of our products to careful function and release testing, although the user still has to perform quality assurance in accordance with general laboratory standards (GLP). GLP requires a careful process including Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ). Merck Millipore offers comprehensive IQ, OQ and PQ documentation for the Spectroquant and Reflectoquant systems, as well as IQ and OQ documents for Turbiquant instruments. The purpose of the Installation Qualification is to verify that the entire delivery matches the purchase order and to assure that the instrument is installed correctly. Installation Qualification documents are available for allreflectoquant, Spectroquant, and Turbiquant instruments. Scope of delivery of Spectroquant Multy colorimeter. 148

149 Your benefits: flawless implementation correct installation comprehensive documentation method-specific checks Operational Qualification is used to assure the functionality of the instruments over the entire operating range, according to the defined procedures. We recommend that you use our extensive range of process control options: System Instrument Functionally Test Product Ord. No. Page Reflectoquant RQflex 10 Check of remission RQcheck Check Set RQflex 10 plus Check of remission RQcheck Check Set Spectroquant Picco Cl 2 /O 3 /ClO 2 /CyA/pH Check of absorbance Reference Standard Multy Check of absorbance Verification Standard NOVA, Pharo Check of absorbance PhotoCheck Pharo 100, Pharo 300 Check of absorbance, stray light, wavelenght UV/VIS Standards Pharo 300 Check of stray light in UV range Turbiquant 1100 IR/T Check of 90 scattered light 1500 IR/T Check of 90 scattered light 3000 IR Check of 90 scattered light 3000 T Check of 90 scattered light UV/VIS Standard Calibration Standard Calibration Standard Calibration Standard Calibration Standard

150 Spectroquant Verification Standard for Spectroquant Multy colorimeter Ord. No The Spectroquant Verification Standard is used to carry out checks of accuracy and reproducibility of measuring results with the Spectroquant Multy colorimeter. The standards are supplied in sealed vials and are individually calibrated on instruments traceable to N.I.S.T. SRM 2032, 935a. The kit contains one Zero standard and 6 cuvettes for checking the 6 different wavelengths of the Multy colorimeter. Find detailed Information about Spectroquant Multy colorimeter on page 73 Verifying the product-related functionalities is the most specific and comprehensive step in the process, and involves the measurement of both method-specific standards and real samples. Performance Qualification consists of two parts: Total System Monitoring and the Matrix Check. Total System Monitoring is performed in order to check the entire system (instrument + test kit + standard + pipette and/or cuvette + operator). It is a method-specific check using standard solutions. The recommended standard solutions for the Reflectoquant system are listed on page 127 and for the Spectroquant system on page 128. With Spectroquant PipeCheck you can check your pipettes and document the measurement results accordingly, even if you don't have a balance. For further information see page

151 The Matrix check is used to check interferences which derive from the sample matrix. As a rule, a sample contains not only the substance to be analyzed, but also foreign substances. Under certain circumstances, the interference these cause can be so great that the recovery of the parameter is significantly less than 100%. For this reason, a variety of foreign substances have been investigated to define the maximum concentration at which they may be present in samples without interfering with the determination in question. These limits are stated in the package insert of each test kit. Some samples have a very complex composition (matrix) or one which is not precisely known. In such cases, it is particularly difficult to estimate the potential influence of the foreign substances on the analysis (matrix effect). That s why the Matrix Check is such an essential part of the whole Analytical Quality Assurance process. There are two methods to choose from, depending on the concentration of the original sample: standard addition (spiking) or dilution of the sample with distilled water. For the standard addition, the recommended standard solutions for the Reflectoquant system on page 127 or for the Spectroquant system on page 128 are also used. Spectroquant NOVA and Pharo photometers provide an instrumentsupported Analytical Quality Assurance System. For further information see page 118 ff. Comprehensive quality assurance using the IQ, OQ, and PQ documents will transform your measurement results into proven, verifiable analytical results. Please contact your local dealer to find out more about this service. 151

152 My customers have high standards and expectations. My job is to exceed them. Ananthasivan K. S., Marketing Manager Like his colleagues at over 60 sites worldwide, Ananthasivan takes his customers success personally. That s why he listens carefully to their needs and challenges then provides individual consultancy and quotations to match. From timely deliveries to quality control and certification services, Ananthasivan goes the extra mile to partner his customers long term. 152

153 Service Service tools 154 Environmental aspects

154 Service Detailed information on all products on the web Simply competent We offer our customers around the world a comprehensive range of services, from detailed information on our website, professional training courses, practical application advice through to issues concerning environmentally compatible disposal. Online Services Explore our large product and service portfolio online. offers you the whole Merck product range. To find more information on water and food analysis products you may visit To look for special photometry information use Quick links Take a look at the quick links leading you to general information on our test kits, such as application finder, photometer method update, or this catalog as a download. 154

155 Search Search for product name, product number or key word. As your are typing your search string, you will get suggestions in a drop-down list. Choose from the list and you will be immediately directed to the corresponding product page. Material Data Sheets and Certificate of Analysis Using the Quick Search feature you get directly to a pre-filled search field for MSDS or CoA. Direct access to product information Entering the six-digit product number will lead you to the corresponding product page. In About this product you will find (depending on the product) instructions for use, applications, technical information documents such as certificates and safety data sheets, and more brochures. Here you will find alternative Merck products for your chosen product above as well as accessories you need! Applications for Reflectoquant and Spectroquant test kits Many of our test kits can be used directly for the analysis of drinking water and for wastewater investigation. There are, however, some areas like food and beverage applications or testing of solid or slurry samples that require special sample pre-treatment measures. Examples Hydroxymethylfurfural in honey, calcium in milk, lipase in cocoa, ascorbic acid in lollipops and candies or chloride in concrete, phosphorus in waste-dump percolating water, boron in soils. To minimize the effort needed for you to conduct such analyses, we keep a stock of more than 300 applications ready for you to assist you in your work. The complete analytical procedure is detailed in the document, from how to prepare samples to determining measurement parameters and comparing the process with other methods. New applications are added on a regular basis. Look for the analytical application finder on our website. Choose either photometry or reflectometry. The tool uses three search parameters field of activity, sample and parameter to locate the applications that users are looking for. Applications are easily converted into PDF files. If you already know the test kits you would like to work with, just enter the six-digit product number in the search field to view the applications listed for that parameter on the product detail page. Photometer Method Update You can update the software on your Merck photometer any time and free of charge! This gives you the assurance that your results will be correct, as it s our responsibility to calibrate the tests for the instrument. Use the same instrument to conduct all new tests without having to program it yourself. Benefit from always having an up-to-date instrument! Look for method update photometer under quick links. 155

156 Service Customized support and safety aspects On-the-spot consultancy In addition to giving advice in matters of analysis and technical instruction by our local specialists, we have also installed competent and practically oriented hotlines in almost all countries throughout the world. Inquiries are answered immediately or else forwarded to our specialists from the development sector. Training courses and seminars Regular training courses are run to give users tips and hints of use in analysis and to help them avoid making errors in their day-to-day operations. These training courses and seminars are always held with a small number of participants to ensure an intensive and lively exchange of ideas. This provides the opportunity to deal with questions raised by the participants individually and in detail. Application laboratory Our central application laboratory works on new applications for our range of test kits. We regularly check developments in the market and your inquiries in order to define the parameters and sample materials that are of most interest. If you do not find an application on our website, please contact us for further consultancy. Research and Development Merck builds upon the foundations of its technological expertise and functional processes - from research to knowledge of our customers demands. From that and from our contacts to national standard committees, we develop market-oriented ideas for new test kits and their applications. Reference laboratory If you wish to compare your results with those yielded by reference methods gained in accredited laboratories, we can offer you this service, too, in collaboration with our independent external partners in the field. Validation and accreditation for Spectroquant test kits If you are planning to validate our Spectroquant test kits and to get accredited, you may contact our local product specialists to support you by providing special checklists and spreadsheets. 156

157 Safety aspects More safety for your lab technicians In developing our test kits, wherever possible we avoid the use of harmful chemicals, e.g. chloroform, cadmium, or benzene. More safety features Identification and safety labels on test kits go without saying. At Merck you find them even on every Spectroquant cell test and on all reagent bottles. This information helps you to avoid any potential risk. Robust test vessels with leak-proof caps ensure safe handling. Of course, our products implement all relevant statutory requirements and we supply comprehensive and easy-to-use waste disposal advice for our customers. More information for safer handling People dealing with laboratory chemicals should be informed about potential risks. Informative Material Safety Data Sheets (MSDS) are available for our test kits free of charge and in over 30 languages. They provide important information and useful advice concerning ingredients, technical handling of chemicals, workplace safety measures and ecological data - topics on which other companies do not offer any information at all or only very rarely. 157

158 Service Customized support and safety aspects Environmental Aspects Merck does more than merely sell chemicals and test kits we recognize our responsibility for our products throughout their entire lifespan. Merck gives top priority to protecting the environment. Therefore, right from the start of development we involve our Environment, Health, Safety, Security and Quality department that is responsible for global coordination of environmental and safety management. And we follow the obligation to respect our environment in many aspects here are some examples: ISO certificate Merck performs environmental and safety audits at all our production sites in order to identify weaknesses and continually improve the utilization of resources. These internal audits are supplemented by additional audits by third parties. As of December 2008, 26 of our sites held ISO certification. Safe shipping Merck ships its products to customers and subsidiaries all over the world. Safety is an overriding objective of our shipments, which should pose no danger to people or the environment. The goods and raw materials should arrive at their destination intact. Since many of the products that Merck ships are classified as dangerous goods, strict transport regulations are adhered to worldwide. Waste disposal With the help from Merck, laboratories can play an active role in protecting the environment: Merck provides the convenient Retrologistik disposal service for safe and simple disposal of chemical waste and package materials. The recycling of used test kits is already a constituent part of our product development. Taking these principles into due consideration, Merck has implemented an extensive and customer-friendly concept for the entire range of Spectroquant products. Based on this concept, not only used packaging materials, but also used chemicals and reagents, are recycled by means of environmentally compatible methods. Waste disposal advice online This new service combines customer support and environmental protection. The step-by-step instructions also given online guide users through a straightforward process on how to properly dispose of used Merck test kits, such as Spectroquant, Aquamerck, Microquant, and Aquaquant, among others. These easy-to-order instructions bring greater uniformity to working practices and more importantly help laboratories play an active role in protecting the environment. 158

159 Together we can protect our world. Come and help us make the future brighter. For years, Merck has been manufacturing products that help to improve our environment and are easier on nature themselves. One of our central goals is to reduce the amount of hazardous or toxic chemicals our test kits contain. As a result, they not only provide precise, reliable results they also help preserve our environment. In addition to our test kits for water and food analytics, we can also supply lowerimpact products for a wide range of other applications. Contact your for details.