3 Petrifilm Environmental Listeria Plate for the Rapid Enumeration of Listeria from Environmental Surfaces
|
|
- Cory Austin
- 6 years ago
- Views:
Transcription
1 3 Petrifilm Environmental Listeria Plate for the Rapid Enumeration of Listeria from Environmental Surfaces Barbara Horter and Henry Lubrant, 3M Microbiology, St. Paul, MN, USA Abstract Accurate and rapid detection of Listeria is of significant interest to the food industry. The 3M Petrifilm Environmental Listeria Plate has been developed in order to provide a Listeria result from environmental samples within 27 to 31 hours. This study compared the Petrifilm Plate method with ISO using PALCAM agar for detection and enumeration of Listeria. Studies of 88 strains demonstrated that the Petrifilm Environmental Listeria Plate has an inclusivity (sensitivity) of 98% and an exclusivity (specificity) of 100% when compared to ISO When comparing colony counts of the strains tested, a paired t-test showed that there was no significant difference between counts on the Petrifilm Plate method and the ISO method (p>0.05). Three different surfaces and nine different collection devices were tested using both methods. The surfaces were artificially inoculated with Listeria and background microbiota. Both sponges and swabs were used as collection devices. After a 60 to 75 minute repair step in buffered peptone water at room temperature (23.7 C), the samples were plated onto Petrifilm Plates and onto PALCAM agar plates. Results showed that, on average, the Petrifilm Plate method was not statistically different (p>0.05) from the ISO method for enumeration of Listeria. Introduction Keeping Listeria out of the food-manufacturing environment is the best defense against product contamination. A vigorous environmental monitoring program is a vital preventative measure in the management of product contamination due to Listeria. The 3M TM Petrifilm TM Environmental Listeria Plate was developed to provide rapid and quantitative Listeria results from environmental samples. The Petrifilm Environmental Listeria Plate is a sample-ready culture medium system containing selective agents, nutrients, a cold-water-soluble gelling agent, and a chromogenic indicator. Petrifilm Environmental Listeria Plates are used for the detection and/or enumeration of Listeria in environmental samples. The sample is collected using a sponge, swab or other pre-moistened sample device. After five milliliters of repair broth - buffered peptone water - is added to the sample, the sample is mixed and then allowed to sit at room temperature for a minimum of 1 hour and up to a maximum of 1.5 hours. The sample is mixed and 3 milliliters is plated onto the Petrifilm Plate. The 1 3Microbiology
2 gelling agent is allowed to solidify after inoculation, and the plate is then incubated for hours at C or C. Red-violet colonies on the plate are Listeria (see Figure 1). Figure 1. Petrifilm Environmental Listeria Plate showing red-violet Listeria colonies There were 2 objectives of this study. One was to compare the Petrifilm Environmental Listeria Plate method with the ISO method for inclusivity of target organisms and exclusivity of non-target organisms. The second objective was to compare the Petrifilm Environmental Listeria Plate method with the ISO method for the detection and enumeration of Listeria from different surface materials. Materials and Method Strain Testing Bacteria for the study 51 Listeria spp. and 37 non-listeria including Bacillus, Brevibacterium, Enterococcus, Erysipelothrix, Escherichia, Kurthia, Lactobacillus, Pediococcus, Pseudomonas, Staphylococcus and Streptococcus were selected from the 3M culture collection or were purchased from the American Type Culture Collection (ATCC; Manassas, VA). Frozen cultures were stored at -84 o C in 15% glycerol. Strains were tested according to the methods outlined in Figure 2. 2
3 Figure 2. Strain testing procedure Grow organisms overnight in TSB about 19 h Dilute organism in Standard Methods Buffer Petrifilm Plate Method ISO Method Add 0.5mL organism to 5 ± 0.5mL BPW Add 0.5ml organism to 4.5 ± 0.5mL BPW 1 hr ± 5 min at 25 ± 2 C 1 hr ± 5 min at 20 ± 2 C 3mL on Petrifilm Listeria Plate 1mL on PALCAM 150mm plate 1mL on PALCAM 150mm plate 28 ± 2 h at 35 ± 1 C h at 37 ± 1 C Red-violet colonies are Listeria Perform confirmation tests Calculate Colony Counts 3 Record colony counts
4 Surface Testing To simulate environmental testing, 45 samples were analyzed which consisted of 3 different surface types, 9 different collection devices and 5 different organisms. Samples were tested using the Petrifilm Environmental Listeria Plate and the ISO method. Surfaces stainless steel glazed earthen tile plastic (polypropylene) Collection devices Hydrasponge TM with 10 ml of neutralizing buffer (International Bioproducts) Hydrasponge TM with 10 ml of letheen broth (International Bioproducts) Hydrasponge TM with 10 ml of buffered peptone water (International Bioproducts) Spongesicle TM with 10 ml neutralizing buffer (International Bioproducts) dry, sterile sponge to which 10 ml of sterile water was added Quick Swab (3M) cotton-tipped swab with wooden shaft (Puritan) dacron-polyester-tipped swab with plastic shaft (Puritan), foam-tipped swab with plastic shaft (VWR) Organisms used in surface testing 2 Listeria monocytogenes Listeria innocua Listeria welshimeri E.coli Procedure Organisms were grown overnight in trypticase soy broth + 0.6% yeast extract. A 4 X 4 inch square area on each sterilized surface was inoculated with organisms a Listeria plus E.coli as background or E.coli by itself. The surfaces were allowed to dry overnight. Pre-moistened sampling devices were used to collect samples by swabbing or sponging the area in both a vertical and horizontal direction. The sampling device was returned to the container it came in or to a sterile test tube and 8 ml of buffered peptone water was added. The sample was either vortexed or stomached. The sample was allowed to sit at room temperature (23.7 C) for 60 to 75 minutes. Just before plating, the samples were remixed. A 3 ml sample was plated onto the Petrifilm Environmental Listeria Plate. One-third of a ml was plated onto each of 9 PALCAM agar plates. 4
5 Petrifilm Plates were incubated for 28 ± 0.5 h and PALCAM plates were incubated for 48 h at 35 ± 1 C. Colony counts were recorded. Data Analysis Inclusivity and exclusivity values were calculated for the Petrifilm Environmental Listeria Plate method as compared to the ISO method. For both the strain testing and the surface testing, raw counts were converted to log 10 counts to more nearly match the underlying assumption of normality. A paired t-test was used to compare the Petrifilm Environmental Listeria Plate method with the ISO method for each portion of the study. Results Strain Testing The inclusivity and exclusivity of the Petrifilm Environmental Listeria Plate method as compared to the ISO method is listed below. Testing was performed on 51 Listeria isolates and 37 non-listeria isolates. The results are shown in Table 1. The following organisms did not grow on either method: Listeria seeligeri ATCC 35967, Listeria grayi ATCC , Listeria monocytogenes ATCC and ATCC J Table 1. Identification of Listeria organisms by the Petrifilm Plate and ISO methods. ISO Petrifilm Environmental Listeria Plate Inclusivity (Sensitivity) = 98% Exclusivity (Specificity) = 100% Table 2 shows the results of the comparison between the Petrifilm Environmental Listeria Plate method and the ISO method for colony counts from strain testing. 5
6 SE c t-value d p-value d Table 2. Comparison of the Petrifilm Plate method with the ISO method for the enumeration of Listeria. Testing N a Mean log Difference b Strains Surfaces a Number of samples used in the analysis b mean log difference: log ISO count-log Petrifilm Plate count c Standard error of the mean log difference d Value associated with the statistical test The mean log Listeria counts from the Petrifilm Plate method were not significantly different from the mean log counts from the ISO method. Surface Testing Results from the comparison between the Petrifilm Environmental Listeria Plate method and the ISO method for colony counts on surface testing are listed in Table 2. The mean log Listeria counts were not significantly different when comparing the log colony counts from the Petrifilm Environmental Listeria Plate method to the log colony counts of the ISO method for surface testing Conclusion Results with the Petrifilm Environmental Listeria Plate method were similar to those of the ISO method for strain and surface testing. The Petrifilm Plate gave results in less than one-third of the time of the reference method. This new Petrifilm Plate method provides the added advantages of a convenient, space-saving and waste-reducing, sample-ready medium. REFERENCES ISO :1998 (E), Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenes Part 2: Enumeration method (1998) International Organization for Standardization, Geneva, Switzerland. AKNOWLEDGEMENTS The authors wish to thank Kathryn Lindberg for statistical consulting. Hydrasponge TM and Spongesicle TM are trademarks of International Bioproducts 3M Microbiology 3M Center, Bldg E-05 St. Paul, MN USA microbiology@mmm.com 6 Petrifilm is a trademark of 3M. 3M 2004
Interpretation Guide. Environmental Listeria Plate
Interpretation Guide The M Petrifilm Environmental Listeria Plate is a sample-ready culture medium containing selective agents, nutrients, a cold-water-soluble gelling agent, and a chromogenic indicator
More information3M Food. with 3M. TB Effective Date: Number: April 2, 2013 Supersedes: New Technology Products Originating.
Food Title: TM Enrichm ment Pouch with Buffered Peptone Water (ISO): Compatibility with TM Sample Handing Devices Number: TB.201318.00 Effective Date: April 2, 2013 Supersedes: New Technology Platform:
More informationtel: fax: foodcheksystems.com
METHOD COMPARISON STUDY REPORT Faster Detection of Listeria spp. by BAX System Real-Time PCR Assay in Environmental Samples Following 20 hours Enrichment with Actero Listeria Enrichment Media Actero Listeria
More informationAssessment of Antibacterial Efficacy of Goldshield against MRSA and VRE
Assessment of Antibacterial Efficacy of against MRSA and VRE Sheri Maxwell, B.S. Charles P. Gerba, Ph.D. Department of Soil, Water and Environmental Science University of Arizona Tucson, Arizona. 85721
More informationtel: fax: foodcheksystems.com
METHOD COMPARISON STUDY REPORT Faster Detection of Listeria Monocytogenes by BAX System Real-Time PCR Assay in Environmental Samples Following 20 hours Enrichment with Actero Listeria Enrichment Media
More informationFinally, You Have a Choice! Online Ordering Available
Simple and Effective System for Colony Counting Finally, You Have a Choice! Online Ordering Available Compact Dry is a ready-touse system reducing the time needed to perform microbial testing on food,
More informationBiocidal Surface Test - Clinell Wipes ~ Project Report Prepared for GAMA Healthcare Ltd ~ Huddersfield Microbiology Services Oct 06
Biocidal Surface Test - Clinell Wipes ~ Project Report Prepared for GAMA Healthcare Ltd ~ Huddersfield Microbiology Services Oct 06 Summary The ability of Clinell wipes to disinfect contaminated steel
More informationPublic Health England (PHE) Certified Reference Materials for Microbiology. Certificate of Analysis
Public Health England (PHE) Certified Reference Materials for Microbiology Certificate of Analysis This certificate should not be parted from the PHE certified reference material with batch number: 1070-14
More informationFoodChek - Listeria spp.
FoodChek - Listeria spp. Sampling Kit Bulk Product Information Intended Use: The FoodChek - Listeria spp. is a lateral flow immunonanomagnetic screening assay for the rapid detection of Listeria spp. from
More informationListeria Right Now. An enrichment-free environmental monitoring tool for Listeria detection providing actionable results in less than 60 minutes.
Listeria Right Now An enrichment-free environmental monitoring tool for Listeria detection providing actionable results in less than 60 minutes. LICENSE NUMBER 081802 Less than an hour? How is this possible?
More informationPuritan Environmental Sampling Kit (ESK )
Puritan Environmental Sampling Kit (ESK ) Table of Contents: Introduction Pg.2 Description..Pg.2 Production Information.... Pg.2-4 Attributes and Intended Uses... Pg.4-5 Common Food and Water Borne Pathogens...Pg.5-6
More informationRapid Aerobic Count. Interpretation Guide. 3M Food Safety 3M Petrifilm Rapid Aerobic Count Plate
3M Food Safety 3M Petrifilm Rapid Aerobic Count Plate Rapid Aerobic Count Interpretation Guide The 3M Petrifilm Rapid Aerobic Count Plate is a sample-ready culture medium system which contains nutrients,
More informationPetrifilm RYM. Interpretation Guide. Rapid Yeast and Mould Count Plate. Brand
Petrifilm Brand Interpretation Guide The 3M Petrifilm Rapid Yeast and Mould Count Plate is a sample-ready culture medium system that contains nutrients supplemented with antibiotics, a cold-water-soluble
More informationFINAL REPORT Efficacy of a Cold Plasma System
FINAL REPORT Efficacy of a Cold Plasma System ORDER Number PREPARED FOR: 714 Mall Blvd., Suite 3 Savannah, GA 31406 EMSL Analytical, Inc. Web: http://www.emsl.com - 1 - Certificate of Analysis Client:
More informationPersistence of Activity of a Hand Sanitizer
Persistence of Activity of a Hand Sanitizer Eurofins Microbiology Laboratories, Inc. 2430 New Holland Pike Lancaster, PA 17601 717-556-7373 www.eurofinsus.com TABLE OF CONTENTS 1.0 OBJECTIVE... 3 2.0 PROTOCOL
More informationThe Efficacy of 300 ppm Peracetic Acid from Perasan MP-2 and MP-2C on E. Coli O157:H7 Inoculated Meat Surfaces
The Efficacy of 300 ppm Peracetic Acid from Perasan MP-2 and MP-2C on E. Coli O157:H7 Inoculated Meat Surfaces Background Joseph Donabed, B.S. Enviro Tech Chemical Services, Inc. October 25 th, 2016 Enviro
More informationRAC. Interpretation Guide. Rapid Aerobic Count Plate
Interpretation Guide The 3M Petrifilm is a sample-ready-culture medium system which contains nutrients, a cold-water-soluble gelling agent and a dual-sensing indicator technology that facilitates aerobic
More informationPublic Health England (PHE) Certified Reference Materials for Microbiology. Certificate of Analysis Clostridium perfringens CRM13170L
Public Health England (PHE) Certified Reference Materials for Microbiology Certificate of Analysis Clostridium perfringens CRM13170L This certificate should not be parted from the PHE certified reference
More informationAlpha HydroMAID Cleaning Effectiveness of the Alpha HydroMAID Cleaning System versus Conventional Mopping
The purpose of these studies was to evaluate the effectiveness of the Alpha HydroMAID (Mobile Automated Integrated Diluter) for removing a bacterial/fungal mixture typical of a kennel floor bioload and
More informationCleaning Efficiency of The MicroOne Wipe and Mop Cleaning System
PDA Laboratories, Inc. A Contract ing Laboratory Visit us at www.pdalabsinc.com 17A Kiel Avenue Kinnelon, NJ 07405 (Tel) 973-492-8000 (Fax) 973-492-8002 pdainfo@pdalabsinc.com Cleaning Efficiency of The
More informationFood Sample Procedure (1:10 or Greater Dilution of Food Sample)
30 25 20 15 10 7.5.5 3M Petrifilm Aerobic Count Plate For detailed WARNINGS, cautions, DISCLAIMER OF WARRANtIES / LIMIted REMEDY, LIMITATION OF 3M LIABILITY, StoRAGE AND DISPoSAL information, and Instructions
More informationLAB. Interpretation Guide. Lactic Acid Bacteria Count Plate
Interpretation Guide The 3M Petrifilm Lactic Acid Bacteria Count Plate is a self-contained, sampleready-culture-medium system which contains nutrients, selective agents, a cold-water-soluble gelling agent
More informationRapidChek SELECT Salmonella Test Kit
RapidChek SELECT Salmonella Test Kit Part #: 7000188-7000197 AOAC Approved Protocols: This test kit s performance was reviewed by AOAC Research Institute and was found to perform to the manufacturer s
More informationNordVal International. c/o Norwegian Veterinary Institute PB 750 Sentrum, N-0106 Oslo, Norway RAPID L. mono
NordVal International / NMKL c/o Norwegian Veterinary Institute PB 75 Sentrum, N-6 Oslo, Norway www.nmkl.org Issued for: RAPID L. mono NordVal No: First approval date: November 5 Extension date: December
More informationActero Listeria Enrichment Media Product Information
Actero Listeria Enrichment Media Product Information INTENDED USE: Actero Listeria Enrichment Media ( Actero Listeria ) is a selective medium, specifically optimized for a 20-30 hours single-step recovery
More information3M Condensation Management Film (CMFi) Hygienic Evaluation of
Hygienic Evaluation of 3M Condensation Management Film (CMFi) Introduction 3M Condensation Management Film (CMFi)* is designed to evaporate intermittent overhead condensation formed during the warm sanitation
More informationTest Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002
Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002 FTTS-FA-002 Antibacterial Textiles for Medical Use Antibacterial Textiles suppress and even kill harmful bacteria
More informationActero Listeria Enrichment Media Product Information
Actero Listeria Enrichment Media Product Information INTENDED USE: Actero Listeria Enrichment Media ( Actero Listeria ) is a selective medium, specifically optimized for single-step recovery and enrichment
More informationResult: COMPLETE Report Date: June 12, 2017
Send to: Dr. Preetha Biswas Neogen Corporation 620 Lancaster Place Lansing, MI 48912 Result: COMPLETE Report Date: June 12, 2017 Customer Name: Neogen Corporation Location of Testing: NSF Ann Arbor Description:
More informationAntimicrobial effectiveness evaluation of Isocide powder coating versus stainless steel plate
Antimicrobial effectiveness evaluation of Isocide powder coating versus stainless steel plate By: Dian Susanti and Alexander Atmadi Abstract Undesirable bioburdens can cause contamination on equipment
More informationAerobic Count. Interpretation Guide. 3M Food Safety 3M Petrifilm Aerobic Count Plate
3M Food Safety 3M Petrifilm Aerobic Count Plate Aerobic Count Interpretation Guide The 3M Petrifilm Aerobic Count (AC) Plate is a ready-made culture medium system that contains Standard Methods nutrients,
More informationNeogen Rapid Listeria Solutions: ANSR -More Sensitive than ISO. Listeria Right Now Results in 1 hour. Antonio Debán Valles
Neogen Rapid Listeria Solutions: ANSR -More Sensitive than ISO Listeria Right Now Results in 1 hour Antonio Debán Valles Microbiology Application Specialist Neogen Corporation The World s leading Animal
More informationActero Salmonella Enrichment Media Product Information
Actero Salmonella Enrichment Media Product Information INTENDED USE: Actero Salmonella Enrichment Media is a selective medium optimized for an improved enrichment of Salmonella spp. from food and environmental
More informationStandard Operating Procedure Title: Stock Suspensions of Micro-Organisms
2. Correct Aseptic technique must be used when performing all Microbiological procedures. 3. All work must be carried out in the Biohazard Cabinet. Table of Contents 2. Positive Identification of Reference
More informationResult:COMPLETE Report Date: December 28 th, 2015
Send to: Clean Water Environmental, LLC 1939 Talamore Court Southeast, Grand Rapids, MI 49546 Dr. Dale Williams Result:COMPLETE Report Date: December 28 th, 2015 Customer Name: Clean Water Environmental,
More informationTest Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces
Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa
More informationActero Salmonella Enrichment Media Product Information
Actero Salmonella Enrichment Media Product Information INTENDED USE: Actero Salmonella Enrichment Media is a selective medium optimized for an improved enrichment of Salmonella spp. from food and environmental
More informationActero Salmonella/STEC Enrichment Media Product Information
Actero Salmonella/STEC Enrichment Media Product Information INTENDED USE: Actero Salmonella/STEC Enrichment Media is a selective medium optimized for an improved enrichment of Salmonella spp. from food
More informationANALYTICAL REPORT: Comparison of the Microbial Recovery Efficacy of QI Medical EnviroTest Paddles versus a Conventional Contact Plate
FOCUS Scientific Services LLC ANALYTICAL REPORT: Comparison of the Microbial Recovery Efficacy of QI Medical EnviroTest Paddles versus a Conventional Contact Plate REPORT: FS-QI-GM-003 Study Manager: Anthony
More informationHeat Shock Proteins in Yeast (2012)
MASSACHUSETTS INSTITUTE OF Technology Department of Biology Heat Shock Proteins in Yeast (2012) Summary Lydia Breen (Stoneham High School) Mary Brunson (Brookline High School) Yeast is a single-celled
More informationSureTect Listeria monocytogenes PCR Assay Workflow NF VALIDATION ISO Extension Study: Method Comparison
SureTect Listeria monocytogenes PCR Assay Workflow NF VALIDATION ISO 16140 Extension Study: Method Comparison Study Report Ana-Maria Leonte, Thermo Fisher Scientific, Wade Road, Basingstoke, Hampshire,
More informationISO INTERNATIONAL STANDARD
INTERNATIONAL STANDARD ISO 18593 First edition 2004-06-01 Microbiology of food and animal feeding stuffs Horizontal methods for sampling techniques from surfaces using contact plates and swabs Microbiologie
More informationSureTect Listeria species PCR Assay Workflow NF VALIDATION ISO Extension Study: Method Comparison
STUDY REPORT SureTect Listeria species PCR Assay Workflow NF VALIDATION ISO 16140 Extension Study: Method Comparison Ana-Maria Leonte Thermo Fisher Scientific, Wade Road, Basingstoke, Hampshire, RG24 8PW,
More informationDecontamination Effectiveness of Esco Celsafe CO2 Incubator Sterilization Feature using High Heat Temperature By Bekti Tri Sumaryati
Decontamination Effectiveness of Esco Celsafe CO2 Incubator Sterilization Feature using High Heat Temperature By Bekti Tri Sumaryati 1. Summary Esco Celsafe CO 2 Incubator sterilization feature, which
More informationTECHNICAL DATASHEET. in compliance with: USP, ISO and all appropriate subsections FQ 106 v3.0 August, 2015
ETIGAM TECHNICAL DATASHEET ETIGAM NSS biological indicator spore strips for radiation Bacillus pumilus This technical report provides relevant data and instructions for use of the NSS biological indicator
More informationElectrolyzed Water. Oklahoma State University
Oklahoma State University Peter M. Muriana, Ph.D. Associate Professor Dept. Animal Sciences & Oklahoma Food & Ag Products Center Oklahoma State University Stillwater, OK 77- -7- TEL; -7- FAX peter.muriana@okstate.edu
More informationCOUNT METHOD 5.0 OBJECTIVES 5.1 INTRODUCTION 5.2 PRINCIPLE. Structure
Food Microbiology EXPERIMENT 5 STANDARD PLATE COUNT METHOD Structure 5.0 Objectives 5.1 Introduction 5.2 Principle 5.3 Materials Required 5.4 Procedure 5.4.1 E-coli Culture 5.4.2 Food Samples 5.5 Observations
More informationSTANDARD OPERATING PROCEDURE FOR MICROBIOLOGICAL EXAMINATION OF CARCASSES BY WET/DRY SWABBING
17/10/08 Page 1 of 11 1. PURPOSE To ensure that the microbiological examination of carcasses, carried out as part of a plant own check programme for the control the general hygiene of food processing operations
More informationFINAL REPORT LOW LEVEL DISINFECTION EVALUATION VIOGUARD KEYBOARD
. Page 1/30 FINAL REPORT LOW LEVEL DISINFECTION EVALUATION VIOGUARD KEYBOARD Subject: Microbial disinfection test, VIOGUARD, Inc keyboard Escherichia coli Document Date of Creation: June 08, 2017 Date
More informationSureTect Salmonella species PCR Assay Workflow NF VALIDATION ISO Extension Study: Method Comparison
STUDY REPORT SureTect Salmonella species PCR Assay Workflow NF VALIDATION ISO 16140 Extension Study: Method Comparison Ana-Maria Leonte Thermo Fisher Scientific, Wade Road, Basingstoke, Hampshire, RG24
More informationKILL TIME STUDIES Antimicrobial Activity of Advanced Cellular Silver (ACS) 200 Using Methicillin resistant Staphylococcus aureus (MRSA)
KILL TIME STUDIES Antimicrobial Activity of Advanced Cellular Silver (ACS) 200 Using Methicillin resistant Staphylococcus aureus (MRSA) Test Solution: ACS 200 Submitted August 19, 2008 September 4, 2008
More informationF - en /11 TEMPO EB (Enterobacteriaceae)
80 003 12596F - en - 2010/11 TEMPO EB (Enterobacteriaceae) For microbiological control only TEMPO EB (Enterobacteriaceae) is an automated test for use with TEMPO, for the enumeration of enterobacteria
More informationSTANDARD OPERATING PROCEDURE FOR MICROBIOLOGICAL EXAMINATION OF CARCASSES BY THE ABRASIVE SPONGE SWABBING METHOD
17/10/08 Page 1 of 12 1. PURPOSE To ensure that the microbiological examination of carcasses, carried out as part of a plant own check programme for the control the general hygiene of food processing operations
More informationDocument No. FTTS-FA-001. Specified Requirements of Antibacterial Textiles for General Use
1. Purpose and Scope This criterion is applicable to the evaluation and testing of antibacterial activity of textile for general use. The quantitative evaluation of antibacterial activity is judged by
More informationTest Method for Efficacy of Copper Alloy Surfaces as a Sanitizer
Test Method for Efficacy of Copper Alloy Surfaces as a Sanitizer Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa (ATCC 15442) Methicillin Resistant
More informationENVR 421 Laboratory #1: Basic Bacteriology Techniques
ENVR 421 Laboratory #1: Basic Bacteriology Techniques Introduction The purpose of this laboratory exercise is to familiarize you with two fundamental bacteriology techniques: the streak plate and the spread
More informationEnterobacteriaceae Kit
iq-check Enterobacteriaceae Kit User Guide Test for the real-time PCR detection of Enterobacteriaceae in food and environmental samples Catalog #12003068 Table of Contents Section 1 Introduction.... 1
More informationMicrobiological Standards for Reusable Plastic Containers within Produce Grower Facilities within Ontario and Quebec
Microbiological Standards for Reusable Plastic Containers within Produce Grower Facilities within Ontario and Quebec Aim To assess the microbiological standard of reusable plastic containers (RPC s) used
More information3M Microbiology. Food safety. maximized. Safety. Processing
3M Microbiology Food safety Processing maximized Safety Food Safety Maximized The importance of food safety Producing safe and healthy food is critical to the success of one of the largest industries worldwide.
More information2.1 Tryptone Soya Broth containing 4% Tween 80 (TSB + T), or another appropriate deactivating broth.
Prepared by: Date: Supersedes: Checked by: Date: Date Issued: Approved by: Date: Review Date: 1. SCOPE AND APPLICATION This general test method applies to the determination of microbiological test method
More informationMicrobiological Standards for Reusable Plastic Containers within Produce Grower Facilities within Ontario and Quebec
Microbiological Standards for Reusable Plastic Containers within Produce Grower Facilities within Ontario and Quebec Aim To assess the microbiological standard of reusable plastic containers (RPCs) used
More informationEVALUATION OF THE HYGIENE STATUS IN SEVERAL MEAT AND DAIRY PROCESSING ESTABLISHMENTS IN R.MACEDONIA
Available online at www.macvetrev.mk Mac Vet Rev ; 6 (): 5 Original Scientific Article UDC: 579.64:67.5(497.7) EVALUATION OF THE HYGIENE STATUS IN SEVERAL MEAT AND DAIRY PROCESSING ESTABLISHMENTS IN R.MACEDONIA
More informationPROFICIENCY TESTING PROGRAMS MICROBIOLOGY
PROFICIENCY TESTING PROGRAMS MICROBIOLOGY A35 Waters: microbiological analyses except Legionella A50 Waters: microbiological analyses Legionella A50a Cooling tower waters: microbiological analyses Legionella
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationPr oject Summar y. Efficacy of cetylpyridinium chloride to reduce E. coli O157:H7 in commercial beef processing plants
Pr oject Summar y Efficacy of cetylpyridinium chloride to reduce E. coli O157:H7 in commercial beef processing plants Principal Investigators: Mick Bosilevac and Mohammad Koohmaraie U.S. Department of
More informationISO/TS TECHNICAL SPECIFICATION
TECHNICAL SPECIFICATION ISO/TS 11133-2 First edition 2003-12-15 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on
More informationLENTICULE discs Certified Reference Materials for Microbiology
LENTICULE discs Certified Reference Materials for Microbiology Produced in double accredited laboratory fulfilling ISO/IEC 17025 and ISO Guide 34 Escherichia coli CRM09001M This certificate should not
More informationFINAL REPORT LOW LEVEL DISINFECTION EVALUATION VIOGUARD KEYBOARD
Test Report: Keyboard disinfection Page 1/31 FINAL REPORT LOW LEVEL DISINFECTION EVALUATION VIOGUARD KEYBOARD Subject: Microbial disinfection test, VIOGUARD, Inc keyboard Klebsiella pneumoniae Document
More informationI - en /01 TEMPO TVC (Total Viable Count)
80 007 12600I - en - 2014/01 TEMPO TVC (Total Viable Count) For microbiological control only TEMPO TVC (Total Viable Count) is an automated test for use with the TEMPO system, for the enumeration of viable
More informationSilliker, Inc. Food Science Center Report RPN Part B. October 30, 2014
Silliker, Inc. Food Science Center Report RPN 17264 Part B October 30, 2014 The Microbiological Shelf-life and Safety of Two Bagels Topped with two Flavors of Cheese Toppers (Tomato Mozzarella & Southwest
More informationZ Competent E. coli Transformation
467PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Z Competent E. coli Transformation (Cat. # GZ 4, GZ 5) think proteins! think G-Biosciences
More informationSubmitted May 16, May 23, 2014 BY:
KILL-TIME PROTOCOL Antimicrobial Activity of Five Silver-based Solutions Using Methicillin-resistant Staphylococcus aureus (MRSA) Test solutions: A, B, C, D, E Submitted May 16, 2014 May 23, 2014 BY: Richard
More informationSlate Steel (Mild Steel) Ceramic
Demonstrating the efficacy of the Dry Ice Blast Cleaning Process against bacterium Escherichia coli, Salmonella Enteritidis, and Listeria monocytogenes on a range of different substrates in association
More informationMICROBIOLOGICAL DETECTION OF E. COLI WITH UNPARALLELED SENSITIVITY. RUG A novel beta-glucuronidase substrate
MICROBIOLOGICAL DETECTION OF E. COLI WITH UNPARALLELED SENSITIVITY RUG A novel beta-glucuronidase substrate E. coli detection Escherichia coli (E. coli) is a Gram negative bacterium that inhabits the intestines
More informationMicrobial Survival. Created on 2/25/ :05 AM
Microbial Survival Introduction The main objectives of this practical are: Verify, or otherwise, the Department of Health recommendation that food be cooked to 70 o C for 2 minutes or an equivalent time-temperature
More informationPublic Health England (PHE) Certified Reference Materials for Microbiology. Certificate of Analysis Salmonella Nottingham CRM07832L
Public Health England (PHE) Certified Reference Materials for Microbiology Certificate of Analysis Salmonella Nottingham CRM07832L This certificate should not be parted from the PHE certified reference
More informationSerial dilution and colony count (Viable count) Pour plate. Spread plate Membrane filtration. Turbidity. Microscopic cell count
Aljawharah Alabbad 2016 Serial dilution and colony count (Viable count) Pour plate Spread plate Membrane filtration Turbidity Microscopic cell count Many studies require the quantitative determination
More informationBiological Consulting Services of North Florida, Inc.
Biological Consulting Services of North Florida, Inc. June 29, 2015 Village Water Filters 1258 Rainbow Drive #2839 Silverthrone, CO 80498 RE: Biological filtration efficacy testing of the filters provided
More informationFINAL REPORT STUDY TITLE
Final Report: Germicidal Spray Test of ZeroMold Plus Page 1 of 18 FINAL REPORT STUDY TITLE Germicidal Spray Test of ZeroMold Plus Disinfectant SOUTHWEST RESEARCH INSTITUTE PROJECT NUMBERS 01.19898.01.001
More informationPublic Health England (PHE) Certified Reference Materials for Microbiology. Certificate of Analysis Legionella pneumophila CRM12821M
Public Health England (PHE) Certified Reference Materials for Microbiology Certificate of Analysis Legionella pneumophila CRM12821M This certificate should not be parted from the PHE certified reference
More informationTest Organisms: Staphylococcus aureus (ATCC 6538) Escherichia coli (ATCC 11229) Pseudomonas aeruginosa (15442)
STUDY TITLE Evaluation of Antimicrobial Activity of UV Illumination/Hydroxyl Generator s: (15442) PRODUCT IDENTITY Odorox Mobile Disinfection Unit Hydroxyl Generator AUTHOR Scott R. Steinagel, B.S. Manager,
More informationSanitization and endotoxin clearance in AxiChrom columns
Application note 8-990-4 AE Chromatography columns Sanitization and endotoxin clearance in AxiChrom columns Columns used in cgmp environments need to maintain very high standards of hygiene. AxiChrom chromatography
More informationPolyskope 1.0 Multiplex Pathogen Detection Assay
Polyskope 1.0 Multiplex Pathogen Detection Assay User Guide Test for the real-time simultaneous PCR detection of E.coli O157 STEC, Salmonella spp. and Listeria monocytogenes in food and environmental samples
More informationASSESSMENT OF THE MICROBICIDAL ACTIVITY OF AN ACCELERATED HYDROGEN PEROXIDE- BASED FORMULATION (AHP-5) AGAINST VRE AND MRSA
Final Report submitted to Virox Technologies Inc. Oakville, Ontario ASSESSMENT OF THE MICROBICIDAL ACTIVITY OF AN ACCELERATED HYDROGEN PEROXIDE- BASED FORMULATION (AHP-5) AGAINST VRE AND MRSA Syed A. Sattar,
More information2. 47 mm grid marked, white sterile 0.45 micron membranes (Millipore or equivalent) 4. Vacuum pump capable of inches of vacuum
Microbiological Methods IX-B- 1 PRESUMPTIVE MEMBRANE FILTER METHOD AND CONFIRMATION OF PSEUDOMONAS AERUGINOSA PRINCIPLE SCOPE Presumptive Pseudomonas bacteria are quantitated by a membrane filter technique,
More informationErin Crowley, Ben Bastin, Megan Boyle, Jonathan Flannery, M. Joseph Benzinger Jr., Patrick Bird, James Agin, David Goins
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 A Comparative Evaluation of the Invisible Sentinel Veriflow
More informationExercise 13 DETERMINATION OF MICROBIAL NUMBERS
Exercise 13 DETERMINATION OF MICROBIAL NUMBERS Introduction When biologists discuss the growth of microorganisms (microbial growth), they are actually referring to population size rather than to the size
More information01/08/2018. Counting Microorganisms. Counting microorganisms. Turbidity Measurements. Relative abundance. Direct counts.
Counting Microorganisms 1 Counting microorganisms 2 Relative abundance Turbidity measurements Direct counts Absolute counts Viable counts Absolute number of growing bacteria Most probale number (MPN) Probable
More informationSanitization of ÄKTA pilot 600 using sodium hydroxide
GE Healthcare Sanitization of ÄKTA pilot 600 using sodium hydroxide The ÄKTA pilot 600 chromatography system is designed to facilitate sanitization, with the high standards imposed by regulatory authorities
More informationCLENDO INDUSTRIAL LABORATORY. CUSTOMER SAMPLE ANALYSIS REPORT Page1 of 8. FDA No CLENDO CONTROL NO. 9081, 9095
CUSTOMER SAMPLE ANALYSIS REPORT Page1 of 8 A. CUSTOMER NAME TECHNICAL SALES INC. B. CLIENT NUMBER 548 C. ADDRESS HC-1 BOX 23223 CAGUAS, PR 725-8918 D. TELEPHONE (787) 272-3345 E. FAX (787) 272-3191 F.
More informationThis study report is published with the express written consent of Antimicrobial Test Laboratories. Business Mobility Now!
mobile Antimicrobial Lab Test Report The Socket SoMo 650Rx handheld computer incorporates antimicrobial materials that provide an extra layer of protection to the device against the spread and multiplication
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More informationá62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS
USP 40 Microbiological Tests / á62ñ Microbiological Examination 1 á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS INTRODUCTION The tests described hereafter
More informationReduction of Listeria monocytogenes Biofilm Formation in Ready-to-Eat Meat Processing Environments
Reduction of Listeria monocytogenes Biofilm Formation in Ready-to-Eat Meat Processing Environments Final Report submitted to the American Meat Institute Foundation November 28, 2002 Amy C. Lee Wong Food
More informationCOLUMBIA AGAR (base)
COLUMBIA AGAR (base) INTENDED USE Columbia Agar is a highly nutritive medium used for the growth and isolation of a large variety of microorganisms, particularly very fastidious bacteria : streptococci
More informationCopper alloys were selected to cover an object was based on its ability to be fabricated and/or surfaced
Running Title:Reduction of the Microbial Burden in the Clinical Environment with Copper Page 1 of 6 SUPPLEMENTAL MATERIALS AND METHODS FABRICATION OF ITEMS SURFACED WITH ANTIMICROBIAL COPPER ALLOYS: Copper
More informationMICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods
Issue No.29 / March 2010 MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods By Dr Vincent TURBLIN, Deputy Regional Market Manager Poultry - CEVA Animal Health Asia Pacific Most
More informationIN THIS SECTION MICROBIOLOGY TESTING EXPERT SOLUTIONS FOR PRODUCT DEVELOPMENT. Bacterial Endotoxin (LAL) Testing
EXPERT SOLUTIONS FOR PRODUCT DEVELOPMENT IN THIS SECTION MICROBIOLOGY TESTING Microbial assays involve a variety of tests, from the determination of the numbers and types of organisms naturally present
More informationL - en /01 TEMPO EC (E. coli)
80 004 12597 L - en - 2015/01 TEMPO EC (E. coli) For microbiological control only TEMPO EC (E. coli) is an automated test for use with the TEMPO system, for the enumeration of Escherichia coli in 22-27
More informationKILL-TIME STUDIES Antimicrobial Activity of Experimental Solutions Using Legionella pneumophila Test Solution: ACS 200 Submitted June 6, 2012
KILL-TIME STUDIES Antimicrobial Activity of Experimental Solutions Using Legionella pneumophila Test Solution: ACS 200 Submitted June 6, 2012 October 11, 2012 PREPARED FOR: Results RNA 1272 South 1380
More information