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1 C R Y O P R E S E R V A T I O N a n d L I F E S C I E N C E I N S T R U M E N T S P E C I A L I S T S C A T A L O G

2 Proudly distributed in Latin America by: a BMT Management, LLC Company "Improving Results, Always"

3 CONTENTS About Biogenics...4 What Our Customers Say...5 FREEZE CONTROL Biofreezer Technology... 6 Preprogrammed Models (CL2200, CL5500, CL8800)... 6 User-Programmable Models (CL3300, CL8800)... 6 How FREEZE CONTROL Systems Work... 7 FREEZE CONTROL Systems Advantages... 8 FREEZE CONTROL CL8800 System CL8800 Controller Specifications FREEZE CONTROL CL3300 System CL3300 Controller Specifications FREEZE CONTROL CL5500 System CL5500 Controller Specifications FREEZE CONTROL CL2200 System CL2200 Controller Specifications Biofreezer System Options Cryochamber Options...18 Cryobath Options...20 Biofreezer System Accessories Uninterruptable Power Supply/Battery Pack...21 Travel Case...21 Thermometers...21 Cryochamber Lids...22 Cryobath Lids...22 Service Contract Biogenics Service Contract...23 Vitrification CVM1 Vitrification Kit...24 Cryostorage and Transport Equipment MVE Cryoport Clinical Media Please see MediCult catalogue Life Science Technology Instruments Stage and Specimen Warmers Portable Incubators Cell Fusion Alarms Microscopes Labeling Systems Laboratory Consumables Biogenics 6-well vented culture dish Brady Miscellaneous Laboratory Consumables IMV B I O G E N I C S 3

4 ABOUT BIOGENICS The industry s most trusted expert Biogenics is a respected global leader in the supply and service of advanced instruments for cryobiology, assisted reproduction and the life sciences. A pioneer in product improvement and innovation, Biogenics understands the technical complexities of products used in cryopreservation and assisted reproduction. Because of our long experience with these sophisticated products, we can offer our customers the kind of unparalleled support available only from the most trusted expert. Who we serve Our instruments and equipment are ideal for clinical, commercial and research applications. These include assisted reproductive technology (ART) and in-vitro fertilization (IVF), embryology laboratories, university and research programs, gene banks, and facilities that freeze cord blood. In multispecies applications, our products are used by commercial embryo transfer practices, veterinary clinics, artificial insemination (AI) breeders, zoology centers, and transgenic embryo producers. Where we began Founded in 1987, Biogenics began as a technical service offering for biomedical instruments. In our early years, we developed the expertise in supporting highly specialized, complex equipment that forms our focused commitment to customer service today. In 1990 we expanded into selling as well as servicing these instruments, leveraging our technical mastery into a complete offering. Today Biogenics is the focal point for the collection and dissemination of key advancements in research and practice in cryopreservation. Our database includes shared information from practitioners around the world. Because we are always listening to laboratory professionals, we continuously improve and update our product line to meet their needs. And we are actively involved in recommending product improvements to our equipment manufacturers. Our commitment to you Biogenics sets the industry standard for service. Only Biogenics offers a five-year warranty on all major products. Biogenics is the only company who submits every instrument it ships to rigorous testing, calibration, and re-testing under a wide range of simulated application environments. Only Biogenics offers individualized consultation after delivery to assure every customer gets the greatest benefit from every new product. Because of our history, we are the only equipment supplier who can consult on software and hardware installation and service issues. 4 B I O G E N I C S

5 WHAT OUR CUSTOMERS SAY Freeze Control cell freezers from Biogenics have proven to be extremely reliable, giving the IVF program good results from cryopreserved embryos. What appeals to me most is their relative simplicity and economy. Lou Jones, well known to the reproductive biology field, has played a large role in establishing the marque and, equally important, backing it up with excellent service. The Rush program is and will be a repeat customer. Richard G. Rawlins, PhD, HCLD Professor and Director IVF/ART Laboratories Rush Centers for Advanced Reproductive Care Rush Medical Center Chicago, IL I have been working with Lou Jones and Biogenics since they first distributed the Freeze Control in 1990, and I am using the current version of this instrument. In addition to the quality of its products, Lou and his company warrant significant praise. I have the utmost respect for Lou. He is very knowledgeable and provides the best customer service. His company is innovative always there to assist in new developments and offer support when asked. Vendors like Biogenics make life much easier and more successful for those of us in the IVF field. Dr. Barry Behr, PhD, HCLD Assistant Professor of OB/GYN, Stanford University School of Medicine Director of IVF and ART Laboratories, Stanford Medical Center Scientific Director of Huntington Reproductive Centers Stanford, CA I have been a client and colleague of Mr. Lou Jones for over 10 years and I have used two models of the Freeze Control unit. These stellar instruments have never failed during operation. The units operate silently, require very little lab space, utilize a minimum of liquid nitrogen and yield consistently high survival rates for human embryos cryopreserved at all stages of development. The survival rate, upon thaw of embryos frozen with the Freeze Control unit, exceeds 90%. I enthusiastically recommend the Freeze Control systems offered through Biogenics. Biogenics also provides excellent customer support, including recalibration with quick turn-around time. I applaud Lou and Biogenics for their cooperation with and support of laboratories functioning in the field of human ART. Thomas B. Pool PhD, HCLD Scientific Director Fertility Center of San Antonio San Antonio, TX The Freeze Control CL863 freezer from Biogenics is an efficient, user-friendly and cost-effective freezing system. At our IVF Clinic it was initially purchased as a backup for a Planer freezer. However, due to its excellent performance, it is now the only unit we use for freezing embryos and semen specimens. I highly recommend the Biogenics freezer based on its performance in significantly improving the pregnancy rates of our patients undergoing frozen embryo transfer cycles. In addition, the technical assistance and customer service of Biogenics is superb. Hema S. Bhakoo PhD, HCLD Director IVF/Andrology Laboratory Infertility & IVF Association of WNY Buffalo, NY My ten-year association with Biogenics and Lou Jones has always been professional and productive. Mr. Jones has been responsive to needs, inquires, suggestions and problems. Having a good understanding of the principals of cryobiology, Mr. Jones is able to address all the issues raised with regards to the products he sells. The company has excellent service and support capabilities. Mr. Jones has anticipated and provided all the additional products required in a cryo-program. He has stayed on top of regulations governing the field, and adapted his products accordingly. Lynette Scott PhD - New England Fertility IVF Lab Director and Consultant B I O G E N I C S 5

6 FREEZE CONTROL BIOFREEZER TECHNOLOGY Biogenics offers a complete line of FREEZE CONTROL Biofreezer systems, precision engineered for cryopreservation of biological specimens. FREEZE CONTROL systems provide a patented and reliable method for heat transfer and temperature regulation in the freezing of biological material for long-term preservation and viable recovery. These controlled-rate, non-pressurized liquid nitrogen systems include a FREEZE CONTROL temperature controller, a cryo- chamber and a cryobath. Options in system configurations are available Biogenics will work with you to determine the most appropriate combination for your specific application requirements. Four models of preprogrammed and/or user-programmed temperature controllers are available. PREPROGRAMMED MODELS (CL2200, CL5500, CL8800) The customer may specify target temperatures, rates, and holds on preprogrammed temperature controller models. Sets of programs are written and stored onto a single memory chip, which is easily user-replaceable. Final program state can be Hold temperature at finish or Freefall at finish. If the customer requires future program changes, a replacement program chip can be provided. Preprogrammed mode offers a level of increased security, because the programs cannot be inadvertently deleted or modified. USER-PROGRAMMABLE MODELS (CL3300, CL8800) CryoGenesis Version 4 or 5 temperature control software is included with user-programmable models. This Windows -based software allows the user to design temperature protocols by generating a set of ramps (including target temperatures, rates, holds, and alarms); manage protocol libraries (save, load, edit); execute protocols (use real time data, allow user interactions); log progress and allow for clinical validation documentation. As with preprogrammed models, final program state may be Hold or Freefall to the temperature of LN 2. 6 B I O G E N I C S

7 HOW FREEZE CONTROL SYSTEMS WORK There are three essential components to every FREEZE CONTROL system: controller, cryochamber, and cryobath. The cryochamber stands directly in a cryobath of liquid nitrogen and is connected to the temperature controller. The controller regulates the temperature of the specimens precisely and reliably. No special installation is required the user can quickly set up the system, use it and store it away. There are five easy steps for using FREEZE CONTROL biofreezer systems: 1. Switch on the controller. 2. Stand the cryochamber in the LN 2 cryobath. 3. Load specimens when the chamber has reached the start temperature. 4. Select and execute the desired temperature program. 5. After a run, load the next batch of specimens and continue. B I O G E N I C S 7

8 FREEZE CONTROL BIOFREEZER TECHNOLOGY FREEZE CONTROL SYSTEM ADVANTAGES ACCURACY The temperature can be precisely specified and accurately maintained at all times. The uniformity of temperature along and between straws or cryovials also ensures greater consistency and greater success. PRECISION TEMPERATURE CONTROL The rate of cooling and thawing is variable, according to user requirements. Temperatures can be held constant at any point within the control range. Controllers can also be used to provide controlled-rate specimen thawing. Thermometry and temperature control circuits are calibrated to accuracies of better than ± 0.1 C. SAFETY Only small amounts of liquid nitrogen are required. There are no pressurized LN 2 vessels, and no alcohol or other volatile, toxic or flammable liquids are used. 8 B I O G E N I C S

9 EFFICIENCY AND ECONOMY Consumption of LN 2 is less than 1 liter per hour. The low power consumption, typically less than 60 watts, makes fail-safe battery power practical. There are no other consumables. Proprietary circuit design and component selection ensure that calibrations have long term stability, contributing to low maintenance and reduced service costs. RELIABILITY The freezers use only solid state integrated circuitry. There are no compressors, solenoids, fans or valves with moving parts to inevitably wear out. The freezers are vibration free and noiseless. VERSATILITY With models using CryoGenesis, practitioners can design and use an unlimited number of temperature programs of varying complexity, with up to 50 ramps and unlimited duration. A signal tone from the controller, computer, or both can be included in the program to alert the operator when intervention is required, for example, at seeding temperature and/or at program finish. In the CL8800 model, up to 16 programs can be preinstalled to allow the unit to operate without a computer; yet the temperature datalogging facility in CryoGenesis is still active, so temperature cycles can be validated even in preprogrammed mode of operation. B I O G E N I C S 9

10 BIOFREEZER SYSTEMS FREEZE CONTROL CL8800 SYSTEM SYSTEM INCLUDES: CL8800 Preprogrammed/Programmable Temperature Controller CryoGenesis V5 software for Windows, including a 60-minute consultation on installation and operation of CryoGenesis Software CC23S Straw Cryochamber LNS, LNL or DX2 Cryobath All necessary cables including AC power cord, RS232 connection for computer (computer not included in system) PRODUCT CODE: CL8800M The FREEZE CONTROL CL8800 system is a user programmable/preprogrammed biofreezer incorporating a controller with temperature acquisition and datalogging capability. Combined with CryoGenesis V5 software for Windows, a CC23S Straw Cryochamber and either the LNS, LNL, or DX2 Cryobath, this system is perfect for use in a busy lab for routine or research applications. When connected to a computer, the CL8800 provides a facility for full validation of actual freezing cycles that can be assigned to specific file names. The CL8800 is fully user-programmable when run from any IBM compatible PC operating under Windows 3.1 or higher, using our Windows software, CryoGenesis V5. The CL8800 can also be preprogrammed to allow the system to operate without a computer. Up to 16 user-specified programs can be preinstalled. When connected to a PC, the CL8800 s built-in temperature acquisition facility allows CryoGenesis to datalog actual specimen temperature. The temperature control range is +40 C to -120 C. Validation of cryopreservation process can also be performed via an analog chart recorder, using the included temperature output jack. The CC23S Straw Cryochamber included in this system accommodates 23 x 0.5ml or 46 x 0.25ml straws. (A range of FREEZE CONTROL cryochambers is available to accommodate different applications. Please see the specifications under Biofreezer System Options.) The CL8800 system includes your choice of a 1.5 liter insulated and covered metal canister LN 2 cryobath for freezing cycles of up to 90 minutes, a 3.8 liter cryobath for freezing cycles up to 3.5 hours, or a 2.3 liter DX2 EPS foam cryobath with PETG clear lid, for freezing cycles up to 2 hours. 10 B I O G E N I C S

11 CL8800 CONTROLLER SPECIFICATIONS GENERAL: Controlled temperature range Monitoring and Recording Temperature warning signals Temperature sensor Temperature display Recorder output Timing Controller - computer communication between +40 and -120 C range between +50 and -196 C ~ ±1.5 C deviation Platinum resistance element Digital, LED, 0.1 C resolution 1mV/ C, 3.5mm Mono/Stereo Jack Digital, quartz crystal RS-232-C, 2400 baud USB1.1 (USB2 compatible) INTERNAL PROTOCOLS: Maximum number of fixed programs 16 Number of temperature steps per program 4096 Minimum temperature step size 0.04 C Maximum duration of programs 233 minutes EXTERNAL PROTOCOLS (Using CryoGenesis V5): Computer Personal Computer running Windows 98SE or higher Max number of ramps per program no limit Minimum temperature step size 0.04 C Duration of programs no limit OTHER: Power consumption less than 60Watt Mains input V, 50/60Hz Fuses 2 x M A 250V Quick Blow Liquid Nitrogen consumption (typical) less than 1 litre per hour Safe operating temp range for Controller +40 to 0 C Safe storage temp range for Controller +50 to -10 C Controller dimensions 195 x 225 x 90mm (WxDxH) Controller weight 1.9Kg Note: Specifications are subject to change without notice. B I O G E N I C S 11

12 BIOFREEZER SYSTEMS FREEZE CONTROL CL3300 SYSTEM SYSTEM INCLUDES: CL3300 Computer-operated, User-programmable Temperature Controller CryoGenesis V4 or V5 software for Windows, including a 60-minute consultation on the installation and operation of CryoGenesis Software CC23S Straw Cryochamber LNS, LNL or DX2 Cryobath All necessary cables including AC power cord, RS232 connection for computer (computer not included in system) PRODUCT CODE: CL3300M The FREEZE CONTROL CL3300 system is a computer- operated and user-programmable biofreezer. Perfect for routine, research or production applications, the 3300 system provides ultimate flexibility in the creation and execution of temperature protocols. This system requires an IBM- compatible computer to function; however, the computer system requirements are minimal. Older computers may be placed in service with this system. The temperature control range for the CL3300 is +40 C to -120 C. The CC23S Straw Cryochamber included in this system accommodates 23 x 0.5ml or 46 x 0.25ml straws. (A range of FREEZE CONTROL cryochambers is available to accommodate different applications. Please see the specifications under Biofreezer System Options.) The CL3300 system includes your choice of a 1.5 liter insulated and covered metal canister LN 2 cryobath for freezing cycles of up to 90 minutes, a 3.8 liter cryobath for freezing cycles up to 3.5 hours, or a 2.3 liter DX2 EPS foam cryobath with PTEG clear lid, for freezing cycles up to 2 hours. 12 B I O G E N I C S

13 CL3300 CONTROLLER SPECIFICATIONS GENERAL Controlled temperature range Temperature warning sound Thermometry system Temperature display Accuracy Timing Controller computer communication Minimum temperature step size Duration of programs Dimensions (WxDxH) Weight Between +40 and -120 C ~1.5 C deviation Platinum RTD Digital, LCD, 0.1 C resolution Better than ±0.1 C Digital, quartz crystal RS-232-C, 2400 baud, serial port required 0.08 C No limit 155 x 175 x 90mm 1.5 kg OTHER Recorder output 1mV / C Power consumption 50 Watts Mains input VAC, Hz Continuous LN 2 consumption Less than 1 liter per hour Safe operating temperature range +40 to 0 C Safe storage temp range +50 to -10 C Note: Specifications are subject to change without notice. B I O G E N I C S 13

14 BIOFREEZER SYSTEMS FREEZE CONTROL CL5500 SYSTEM SYSTEM INCLUDES: CL5500 Preprogrammed Temperature Controller with up to 8 programs CC23S Straw Cryochamber LNS, LNL or DX2 Cryobath All necessary cables including AC power cord PRODUCT CODE: CL5500M The FREEZE CONTROL CL5500 system is a preprogrammed biofreezer incorporating a temperature controller with built-in rechargeable battery for power failure protection or portable operation. This controller also can be powered from an external 12-volt source. The 5500 offers complete peace of mind when operating in areas of questionable AC mains power. The CL5500 system comes with eight standard or custom resident programs. The CL5500 system is ideal for routine lab applications where the range of controlled temperature will not exceed -43 C. This system is also perfect for the mobile embryo transfer practitioner. For this application, a custom formfitted transport case built to withstand the rigors of road or air travel is available. This compact system, with its built-in power pack, offers failsafe protection against power failures or blackouts. The specimens being cryopreserved will never be affected by such incidents. From a full charge, running time is 3 to 4 hours (continuous) in absence of AC mains power. The temperature control range for the CL5500 is +20 C to -43 C. The CC23S Straw Cryochamber included in this system accommodates 23 x 0.5ml or 46 x 0.25ml straws. (A range of FREEZE CONTROL cryochambers is available to accommodate different applications. Please see the specifications under Biofreezer System Options.) The CL5500 system includes your choice of a 1.5 liter insulated and covered metal canister LN 2 cryobath for freezing cycles of up to 90 minutes, a 3.8 liter cryobath for freezing cycles up to 3.5 hours, or a 2.3 Liter DX2 EPS foam cryobath with PTEG clear lid, for freezing cycles up to 2 hours. 14 B I O G E N I C S

15 CL5500 CONTROLLER SPECIFICATIONS GENERAL Controlled temperature range Between +20 C and -43 C Temperature warning (LED or sound) ~1.5 C deviation Thermometry system Platinum RTD Temperature display Digital, LCD, 0.1 C resolution Accuracy Better than ±0.1 C Timing Digital, quartz crystal Analog temperature output 1mV per C Dimensions (WxDxH) 220 x 192 x 118mm Weight 4 kg PROGRAMS Maximum number of fixed programs 8 Number of steps per program 256 Minimum temperature step size 0.25 C Maximum duration of programs 160 minutes BATTERY PACK Battery charge Battery type Battery Dimensions Charging rate 12V, 7.2Ah Sealed lead acid, absorbed glass mat (AGM) 150 x 100 x 65mm ~1 Amp OTHER Power consumption Less than 60 Watts ( V or V, 50/60Hz) Running time from full charge 3 to 4 hours, depending on program Continuous LN 2 consumption Less than 1 liter per hour Safe storage temperature range +40 C to 0 C Note: Specifications are subject to change without notice. B I O G E N I C S 15

16 BIOFREEZER SYSTEMS FREEZE CONTROL CL2200 SYSTEM SYSTEM INCLUDES: CL2200 Preprogrammed Temperature Controller CC23S Straw Cryochamber LNS, LNL or DX2 Cryobath All necessary cables including AC power cord PRODUCT CODE: CL2200M The FREEZE CONTROL CL2200 system is an elegantly straightforward biofreezer that incorporates a preprogrammed temperature controller. The CL2200 system comes with eight standard or custom resident programs. Extremely simple to operate, this system is the most affordable available and has a temperature range suitable for most applications. The temperature control range for the CL2200 is +20 C to -43 C. The CC23S Straw Cryochamber included in this system accommodates 23 x 0.5ml or 46 x 0.25 ml straws. (A range of FREEZE CONTROL cryochambers is available to accommodate different applications, with cooling rates appropriate for most protocols. Please see the specifications under Biofreezer System Options.) The CL2200 system includes your choice of a 1.5 liter insulated and covered metal canister LN 2 cryobath for freezing cycles of up to 90 minutes, a 3.8 liter cryobath for freezing cycles up to 3.5 hours, or a 2.3 liter DX2 EPS foam cryobath with PTEG clear lid, for freezing cycles up to 2 hours. A custom form-fitted transport case built to withstand the rigors of road or air travel is available. 16 B I O G E N I C S

17 CL2200 CONTROLLER SPECIFICATIONS GENERAL Controlled temperature range Temperature warning sound Thermometry system Temperature display Accuracy Timing Dimensions (WxDxH) Weight Between +20 and -43 C ~1.5 C deviation Platinum RTD Digital, LCD, 0.1 C resolution Better than ±0.1 C Digital, quartz crystal 155 x 175 x 90mm 1.5 kg PROGRAMS Number of fixed programs 8 Number of temperature steps per program 1,024 Minimum temperature step size 0.25 C Maximum duration of programs NORMAL 160 minutes FAST 40 minutes OTHER Power consumption 50 Watt ( VAC, Hz) Continuous LN 2 consumption Less than 1 liter per hour Safe temperature range for cryochamber +50 to -196 C Safe operating temperature range for controller +40 to 0 C Safe storage temperature range for controller +50 to -10 C Note: Specifications are subject to change without notice. B I O G E N I C S 17

18 BIOFREEZER SYSTEM OPTIONS Biogenics FREEZE CONTROL biofreezer systems consist of a temperature controller, cryochamber, and cryobath. A range of FREEZE CONTROL cryochambers is available to accommodate different specimen containers (e.g., straws and/or cryovials, security straws, etc.), with cooling rates appropriate for virtually any protocol. There are two cryobath sizes available, interchangeable according to customer preference. CRYOCHAMBER OPTIONS FREEZE CONTROL cryochambers are unique. Their patented design ensures symmetrical heat transfer from all specimens to liquid nitrogen. The highly conductive material used for the chamber ensures a very high degree of temperature uniformity of each specimen whether Liquid Nitrogen levels are high or low. Specimen temperature is monitored continuously and is measured by a precision platinum temperature sensor permanently mounted in the core. Specimens can be safely accessed for manual seeding, without exposing them to temperature fluctuations. The conductive core of the chamber, and the tight thermal coupling between specimens and core, ensure that latent heat is efficiently removed during the nucleation process. All cryochambers are available in two styles: STANDARD or FAST, designated by product code suffixes S or F, respectively. The STANDARD design is suitable for most applications, whereas the FAST models are appropriate for applications where a higher- speed cooling rate is desired. We are pleased to guide you in the selection of cryochambers to match your requirements. The Standard CC23S Straw Cryochamber is included with all biofreezer systems unless the customer order states otherwise. STANDARD CC23S STRAW CRYOCHAMBER CC23S The CC23S Cryochamber accommodates 23 x 0.5ml, or 46 x 0.25ml straws. PRODUCT CODE: CC23S STANDARD FAST Maximum Cooling Rate at 20 C 8 C/minute 16 C/minute Maximum Cooling Rate at -40 C 5 C/minute 10 C/minute 18 B I O G E N I C S

19 CRYOCHAMBER OPTIONS VHC CRYOVIAL / HIGH CAPACITY STRAW CRYOCHAMBERS Biogenics offers several options in VHC cryochambers for use with any of our biofreezer systems. The VHC (Variable High Capacity) has a convertible design, allowing freezing of up to 15 x 1.0ml Nunc cryovials, or up to 85 x 0.25ml or 55 x 0.5ml straws (with interchangeable lifters). VHC CC5A The CC5A 5-slot cryochamber accommodates 15 x 1ml, 10 x 2ml, or 5 x 5ml cryovials. PRODUCT CODE: CC5A STANDARD FAST Maximum Cooling Rate at 20 C 6 C/minute 12 C/minute Maximum Cooling Rate at -40 C 3 C/minute 6 C/minute VHC CC85S The CC85S 5-slot cryochamber accommodates 85 x 0.25ml straws. PRODUCT CODE: CC85S STANDARD FAST Maximum Cooling Rate at 20 C 6 C/minute 12 C/minute Maximum Cooling Rate at -40 C 3 C/minute 6 C/minute VHC CC55S The CC55S 5-slot cryochamber accommodates 55 x 0.5ml straws. PRODUCT CODE: CC55S STANDARD FAST Maximum Cooling Rate at 20 C 6 C/minute 12 C/minute Maximum Cooling Rate at -40 C 3 C/minute 6 C/minute B I O G E N I C S 19

20 BIOFREEZER SYSTEM OPTIONS CRYOCHAMBER OPTIONS CC60 The CC60 is excellent for high volume applications. This high capacity, single-tiered cryochamber holds up to 60 x 1.0ml Nunc cryovials and comes equipped with an LNL 3.8 liter cryobath. (Note: The CC60 will not operate with the smaller LNS cryobath). PRODUCT CODE: CC60 STANDARD FAST Maximum Cooling Rate at 20 C 3 C/minute 5 C/minute Maximum Cooling Rate at -40 C 1.5 C/minute 3 C/minute CC20S The CC20S cryochamber is a multipurpose chamber: It accommodates both security and conventional straw types. It holds up to 20 CryoBioSystem security straws. Alternatively, it holds 40 x 0.5ml or 60 x 0.25ml straws. PRODUCT CODE: CC20S STANDARD FAST Maximum Cooling Rate at 20 C 7 C/minute 14 C/minute Maximum Cooling Rate at -40 C 4 C/minute 8 C/minute CRYOBATH OPTIONS LNS 1.5 liter insulated and covered metal canister LN 2 cryobath for freezing cycles of up to 90 minutes. PRODUCT CODE: LNS LNL 3.8 liter insulated and covered metal canister LN 2 cryobath for freezing cycles of up to 3.5 hours. PRODUCT CODE: LNL LNS LNL DX2 2.3 liter EPS foam cryobath with PETG clear lid for increased LN 2 economy. For freezing cycles up to 2.25 hours, eliminating the need for refills during a freeze. Vent holes allow easy access for seeding instruments. PRODUCT CODE: DX2 DX2 20 B I O G E N I C S

21 BIOFREEZER SYSTEM ACCESSORIES Biogenics carries a full range of accessory products to augment any FREEZE CONTROL biofreezer system. CL-P10 UNINTERRUPTABLE POWER SUPPLY/ BATTERY PACK The CL-P10 power pack was engineered to provide constant power protection for most FREEZE CONTROL biofreezers manufactured since May It provides 100% power failure protection for over three hours on a single charge. The CL- P10 operates from its internal battery, from mains, or from an external 12-volt source. The size of the CL-P10 is 121 x 184 x 241mm. It weighs 5.7 kg with the battery installed and has a conveniently placed carrying handle. Custom travel cases are available in a variety of configurations to accommodate the CL-P10 with any model freezer. PRODUCT CODE: P10 TRAVEL CASE Custom form-fitted, ATA-approved transport case for biofreezer systems is built to withstand the rigors of road and air travel. The case incorporates heavy duty hardware, internal bracing, and ABS exterior over plywood laminate. Compartments to house accessories are included. Travel case available for all models of biofreezers and incubators. PRODUCT CODE: CLZ THERMOMETERS TEGAM 871A EXTERNAL OUTPUT DIGITAL THERMOMETER AND K-TYPE PROBE TEGAM 871A interfaces with a chart recorder for independent temperature monitoring. External output is scaled to 1mV per C. Comes complete with cable. PRODUCT CODE: 871A B I O G E N I C S 21

22 BIOFREEZER SYSTEM ACCESSORIES THERMOMETERS L1040 DUAL K-TYPE DIGITAL THERMOMETER The L1040 is an accurate, economical and reliable way to double-check temperatures in any freezer, thaw bath, warm stage or other laboratory device, and it provides the practitioner with precise verification of actual thermal or cryogenic conditions. The extremely small K-type probe (0.060") allows its integration inside a standard specimen carrier, such as a straw or cryovial. It provides the system with a temperature range of -250 C to 1372 C with an accuracy of ±0.1% of reading ±1 C across its range. PRODUCT CODE: L1040 TL13 DATALOGGER MODULE FOR CL3300, CL3000, CL863 Allows recording of specimen temperatures by interfacing the advanced features in CryoGenesis V4 Software with FREEZE CONTROL temperature controllers CL3300, CL3000 and CL863. PRODUCT CODE: TL13 XTL EXTENDED CRYOCHAMBER LIDS Extended lid for FREEZE CONTROL cryochambers accommodates straws with plug-ends or doublelength straws. PRODUCT CODE: XTLT PRODUCT CODE: XTLM XTLT XTLM CRYOBATH LIDS BLS LNS standard replacement lid. PRODUCT CODE: BLS BLH LNS cryobath lid with center hole and offset handle to accommodate chambers equipped with extended lids. PRODUCT CODE: BLH BLC PTEG clear cryobath lid with integral handle for DX2 bath. PRODUCT CODE: BLC 22 B I O G E N I C S

23 SERVICE CONTRACT BIOGENICS SERVICE CONTRACT FOR FREEZE CONTROL Biogenics Service Contract increases the economy, convenience, and efficiency of servicing your laboratory cryosystem. Annual calibration and servicing of all Freeze Control biofreezers and incubators is recommended to ensure continued accurate and trouble-free operation and validation of the equipment. Biogenics is the only company in North America authorized to service Freeze Control. Biofreezer servicing includes a 15-point quality assurance protocol which eliminates any possible drift in previously calibrated settings, scrutinizes your cryochamber for the development of any abnormal temperature gradients, and allows us to compare the values of previous settings to evaluate any performance degradation. Further, diligent QA is mandated by CAP, and is a general part of the overall CLIA guidelines. Inspectors routinely require documentation that such service is being maintained. The Biogenics Service Contract provides the above for a term of five years, plus the following: Dramatic reduction in annual cost Guaranteed lock-in of service rates for five years Guaranteed overnight* loaner system provided free-of-charge (less shipping) in the event of a system breakdown All labor costs for calibration, QA, and repairs are covered Automatic notification of when calibration is due Priority service to contract clientele Peace of mind and less administrative work (no P/O s to issue, invoices to pay, or checks to write) All machines should be serviced once a year, with or without the contract. With the contract, you save money, and everyone saves administrative time. * Weekends excluded PRODUCT CODE: BGSCV8K8 B I O G E N I C S 23

24 VITRIFICATION CVM1 VITRIFICATION KIT The CVM1 Vitrification system was developed by Cryologic to uniquely overcome many of the pitfalls of traditional vitrification. With the CVM1 system your specimens and the vitrification media never touch liquid nitrogen during vitrification or in storage thereafter. This eliminates exposure of the specimens to undesirable elements, viruses, fungi, and other agents, which may be present in liquid nitrogen. Brilliantly designed with the aspect of repeated production in mind, the system consists of a sterilizable metal block which is comprised of a proprietary alloy and coating engineered for maximum cooling rate and specimen protection. Complementary aspects of the system are the unique, proprietary fibre-plugs and their accompanying protective sheaths. The small hook on the end of the fibre-plug is designed to effectively suspend a micro-droplet of vitrification media containing the specimen (eg embryo or ocyte). FEATURES AND ADVANTAGES: No liquid nitrogen exposure Ultra-rapid cooling and thawing of specimens No straw or vapor barriers to heat exchange Convenient handling of specimens Avoidance of de-vitrification Sterilizable parts Convenient storing of specimens Minimal thawing medium required Call or us for a free demonstration video CD of the CVM1 System. PRODUCT CODE: CVM1KIT 24 B I O G E N I C S

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26 CRYOSTORAGE AND TRANSPORT EQUIPMENT Biogenics carries the complete line of cryostorage and transport products by MVE and Taylor-Wharton. MVE SC SERIES, LIQUID NITROGEN FREEZERS The SC Series is designed for the user who has small capacity needs but requires long-term storage and low liquid nitrogen consumption in a convenient lightweight package. SC SERIES SPECIFICATIONS MODELS SC 3/3 SC 8/5 SC 11/17 SC 16/11 SC 20 SC 20/20 SC 36/32 SC 33/26 MAXIMUM STORAGE CAPACITY Number of canisters No. of 1/2 cc straws (10/cane) No. of 1/2 cc straws (1 Level Bulk) No. of 1.2 & 2.0 ml vials (5/cane) PERFORMANCE Liquid nitrogen capacity (liters) Static evaporation rate (liters/day)* Normal working duration (days)** UNIT DIMENSIONS Neck opening [in/(mm)] 2/(51) 2/(51) 2/(51) 2/(51) 2/(51) 2/(51) 2/(51) 2/(51) Overall height [in/(mm)] 16/(406) 18.5/(470) 21.6/(549) 17.5/(444) 25.7/(652) 25.7/(652) 25.9/(657) 25.9/(657) Outside diameter [in/(mm)] 8.7/(222) 10.2/(260) 10.2/(260) 17.2/(438) 14.5/(368) 14.5/(368) 18.2/(464) 18.2/(464) Canister height [in/(mm)] 5/(127) 5/(127) 11/(279) 5/(127) 11/(279) 11/(279) 11/(279) 11/(279) Canister diameter [in/(mm)] 1.5/(38) 1.5/(38) 1.5/(38) 1.5/(38) 1.5/(38) 1.5/(38) 1.5/(38) 1.5/(38) Weight empty [lbs (kg)] 8/(3.6) 12/(5.3) 17/(7.7) 14/(6.4) 26/(11.8) 26/(11.8) 34/(15.4) 34/(15.4) Weight full [lbs (kg)] 14.4/(6.5) 27/(12.1) 36.6/(16.6) 43/(19.6) 62.5/(28.3) 62.5/(28.3) 93.4/(42.4) 93.4/(42.4) ** Static evaporation rate and static holding time are nominal. Actual rate and holding time will be affected by the nature of container use, atmospheric conditions, and manufacturing tolerances. ** Normal working duration is an arbitrary reference, to estimate container performance under normal operating conditions. Actual working time may vary due to current atmospheric conditions, container history, manufacturing tolerances and any individual patterns of use. 26 B I O G E N I C S

27 MVE XC SERIES, LIQUID NITROGEN FREEZERS The XC Series is designed for the user who requires large-capacity storage and low liquid nitrogen consumption in a convenient lightweight package. XC SERIES SPECIFICATIONS MODEL XC 20/20 XC21/6 XC22/5 XC32/8 XC33/22 XC34/18 XC35/12 XC43/28 XC SQ XC47/11-6 XC47/11-10 MAXIMUM STORAGE CAPACITY Number of canisters Sq No. of 1/2 cc straws (10/cane) 780 N/A 2,400 2,520 1,260 2,100 2,800 1,260-4,500 3,500 No. of 1/2 cc straws (1 Level Bulk) ,870 3,666 3,960 1,764 3,000 4,400 1,764-6,216 5,000 No. of 1.2 & 2.0 ml vials (5/cane) 210 N/A ,320 1,050 No. of racks (25 Vials) - 6 (5/2) PERFORMANCE Liquid nitrogen capacity (liters) Static evaporation rate (liters/day)* Normal working duration (days)** UNIT DIMENSIONS Neck opening [in/(mm)] 2.18/(55.4) 3.5/(89) 3.81/(97) 3.81/(97) 2.75/(70) 3.5/(89) 4/(102) 2.75/(70) 5/(127) 5/(127) 5/(127) Overall height [in/(mm)] 25.7/(652) 17.2/(438) 22/(559) 21.5/(546) 26/(660) 26.6/(675) 25/(635) 26.4/(670) 26.5/(673) 26.5/(673) 26.5/(673) Outside diameter [in/(mm)] 14.5/(368) 18.2/(464) 14.5/(368) 18.2/(464) 18.2/(464) 18.2/(464) 18.25/(456) 20/(508) 20/(508) 20/(508) 20/(508) Canister height [in/(mm)] 11/(279) 5/(127) 11/(279) 11/(279) 11/(279) 11/(279) 11/(279) 11/(279) 11/(279) 11/(279) 11/(279) Canister diameter [in/(mm)] 1.65/(41.9) 2.75/(70) 3.09/(79) 2.62/(67) 2.22/(56) 2.81/(71) 2.6/(66) 42.22/(56) 4/(102) 4/(102) 4/(102) Weight empty [lbs(kg)] 26/(11.8) 59.4/(26.9) 26/(11.8) 30/(13.6) 34/(15.4) 34/(15.4) 34/(15.4) 36/(16.4) 36/(16.4) 36/(16.4) 36/(16.4) Weight full [lbs(kg)] 62.5/(28.3) 62.5/(28.3) 66/(30) 87/(39.5) 94/(42.5) 96/(43.5) 95/(43.1) 111/(50.5) 120.4/(54.6) 120.4/(54.6) 120.4/(54.6) ** Static evaporation rate and static holding time are nominal. Actual rate and holding time will be affected by the nature of container use, atmospheric conditions, and manufacturing tolerances. ** Normal working duration is an arbitrary reference, to estimate container performance under normal operating conditions. Actual working time may vary due to current atmospheric conditions, container history, manufacturing tolerance and any individual patterns of use. B I O G E N I C S 27

28 CRYOSTORAGE AND TRANSPORT EQUIPMENT MVE CRYOSYSTEM SERIES The CryoSystem Series is the next generation of large aluminum freezers designed specifically for vial storage. The MVE Cryosystem 2000, 4000 and 6000 combine the benefits of low nitrogen consumption with mid-range vial capacity. The light weight and low space demands of these containers make them the most economical units in their class. CRYOSYSTEM SERIES SPECIFICATIONS MODEL CRYOSYSTEM 2000 CRYOSYSTEM 4000 CRYOSYSTEM 6000 MAXIMUM STORAGE CAPACITY Number of racks Boxes Boxes per rack vials PERFORMANCE Liquid nitrogen capacity (liters) Static evaporation rate (liters/day)* Working volume (liters) Normal working days** UNIT DIMENSIONS Neck opening (in/mm) 8.5/(216) 8.5/(216) 8.5/(216) Overall height (in/mm) 27.5/(680) 37.5/(950) 37.5/(950) Outside diameter (in/mm) 22/(559) 22/(559) 26/(665) Weight empty (lbs/kg) 76/(34.5) 114/(51.7) 125/(56.7) Weight full (lbs/kg) w/ln2 16/(75.3) 312/(141.5) 418/(190) ** Static evaporation rate and static holding time are nominal. Actual rate and holding time will be affected by the nature of container use, atmospheric conditions, and manufacturing tolerances. ** Normal working duration is an arbitrary reference, to estimate container performance under normal operating conditions. Actual working time may vary due to current atmospheric conditions, container history, manufacturing tolerances and any individual patterns of use. 28 B I O G E N I C S

29 MVE VAPOR SHIPPERS, -150 C SPECIMEN TRANSPORT The MVE Vapor Shipper containers are designed for the safe transportation of biological samples at cryogenic (-150 C) temperatures. Fabricated from durable, lightweight aluminum, they contain a hydrophobic absorbent that contains the liquid nitrogen. The absorbent also repels moisture and humidity, assuring the maximum holding time. A protective shipping carton for all models that protects the container from being placed on its side and helps in withstanding the rigors of transportation is available. These containers can be used to ship samples with a nonhazardous classification throughout the world. VAPOR SHIPPERS SPECIFICATIONS MINI CRYO CRYO CRYO MODEL SC 2/1V SC 4/2 V SC 4/3V SC 20/12V XC 14/2V XC 20/3V** MOOVER MOOVER SHIPPER SHIPPER XC MAXIMUM STORAGE CAPACITY Number of canisters Center Rack - No. of 1/2 cc straws (10/cane) ,000 2,500/2,000** No. of 1/2 cc straws (1 Level Bulk) ,500 3,750/3,000** Number of 1/4 cc straws (1 Level Bulk) ,000 6,750/6,000** No. of 1.2 & 2.0 ml vials (5/cane) /600** No. of 1.2 & 2.0 ml vials (6/cane) /720** (Bulk) Number of blood bags stored (4R9953) PERFORMANCE Liquid nitrogen capacity (liters) Static evaporation rate (liters/day)* Static holding time (days) UNIT DIMENSIONS Neck opening [in/(mm)] 1.4/(35) 2.75/(70) 2/(51) 2/(51) 3.81/(97) 3.81/(96.7) 1.4/(35) 3.8/(97) 8.5/(216) 8.5/(216) Overall height [in/(mm)] 13.5/(343) 18.4/(468) 19.4/(492) 25.7/(652) 22.0/(559) 25/(635) 19.5/(495) 22/(558) 21.5/(546) 23/(584) Outside diameter [in/(mm)] 7.25/(184) 8.7/(222) 8.7/(222) 14.5/(368) 14.5/(368) 14.50/(368) 7.2/(184) 18.3/(464) 14.5/(369) 15/381) *** Canister height [in/(mm)] 5/(127) 11/(278) 11/(278) 11/(279) 11/(278) 11/(278) 11/(278) 11/(278) /(317.5) Canister diameter [in/(mm)] 1.2/(31) 2.62/(67) 1.81/(46) 1.5/(38) 3.7/(94) 3.2/(80) 1.2/(31) 3.1/(79) - - Weight empty [lbs (kg)] 6/(2.7) 11/(5) 13/(5.9) 30/(13.6) 24/(10.9) 23/(50.5) 88/(4) 30.5/(13.8) 24/(10.9) 30/(13.6) Weight full [lbs (kg)] 8.8/(4) 18/(8.1) 20.6/(9.3) 52/(23.6) 39/(18 ) 35/(77) 11.6/(5) 38/(17.2) 37.5/(17) 47/(103.4) *** Static evaporation rate and static holding time are nominal. Actual rate and holding time will be affected by the nature of container use, atmospheric conditions, and manufacturing tolerances. *** With center absorbent canister (3-week holding time) *** Useable Height B I O G E N I C S 29

30 CRYOSTORAGE AND TRANSPORT EQUIPMENT MVE LAB SERIES The MVE Lab Series of cryogenic dewars are an efficient, economical, and convenient way to store and dispense liquid nitrogen. Many lab units can be fitted with pouring spouts, pressurized dispensing devices or dippers to aid in the transfer of liquid nitrogen. LAB SERIES SPECIFICATIONS SS TRANSFER MODEL LAB 4 LAB 5 LAB 10 LAB 20 LAB 30 LAB 50 UNIT Net Capacity (liters) PERFORMANCE Static evaporation rate (liters/day) N/A UNIT DIMENSIONS Neck opening (in/mm) 1.4/(35.5) 2.2/(56) 2.2/(56) 2/(51) 2.5/(64) 2.5/(64) 6/(152) Usable height (in/mm) 7.8/(198) 10.5/(266) 13.5/(343) 13.7/(348) 14.9/(378) 22/(559) 14/(356) Overall height (in/mm) 16.8/(426) 18.2/(462) 21.5/(546) 24.7/(627) 24.1/(611) 30.7/(779) 16.5/(419) Outside diameter (in/mm) 7.3/(185) 8.8/(222) 10.3/(260) 14.5/(368) 17/(432) 17/(432) 8/(203) Internal diameter (in/mm) 5.5/(139) 6.5/(165) 8.3/(210) 11.4/(289) 14/(356) 14/(356) 6/(152) Weight empty (lbs/kg) 6/(2.7) 8/(4) 13/(6) 19/(9) 27/(12) 34/(15) 11/(5) Weight full (lbs/kg) 13/(6) 17/(8) 31/(14) 56/(26) 84/(38) 123/(56) 20/(9) 30 B I O G E N I C S

31 CRYOPORT PSX1000 LIGHTWEIGHT LN2 DRY VAPOR SHIPPER Utilizing the R & D advances in the aerospace industry and new materials technologies, CryoPort Stystems has developed a unique lightweight and easy-to-handle nitrogen dry vapor shipper. Designed for the safe transportation of semen and embryos at cryogenic temperatures below -150º, the PSX1000 is easy to use, retains functionality when tipped on its side, and sells for less than other IATA approved dry vapor shippers. SPECIFICATIONS Dry weight: Full weight: Height: Diameter: Opening: Capacity: 11.5 lbs lbs 24 inches inches 2.8 inches 5.5 Liters CAPACITY OF ONE CRYOPORT CANISTER 290 1/2 cc straws (10 per cane) 640 1/4 cc straws (13 mm goblets on canes 2 levels) 320 1/2 cc straws (13 mm goblets on canes 2 levels) 460 1/4 cc straws (10 mm goblets on canes 2 levels) 230 1/2 cc straws (10 mm goblets on canes 2 levels) 740 1/2 cc straws (bulk in 2 layers with lifter) /4 cc straws (bulk in 2 layers with lifter) ml to 2.0 ml vials (5 per cane) PRODUCT CODE: PSX1000 CRYOPORT PS1000 LN2 DRY SHIPPER CryoPort s new personal LN2 Dry vapor shipper, is lightweight, economical, and is ergonomically designed for maximum comfort and ease of use. The PS1000 features an over-the-shoulder strap and easy pour-off handles and document pouch. SPECIFICATIONS Dry weight: Full weight: Height: Diameter: Opening: Capacity: Operating hold time: 7.5 lbs lbs inches inches 2.80 inches Up to 80 vials (16 canes) Up to 16 days PRODUCT CODE: PS1000 B I O G E N I C S 31

32 LIFE SCIENCE TECHNOLOGY INSTRUMENTS STAGE AND SPECIMEN WARMERS TOKAI HIT THERMO PLATE The Thermo Plate, a microscope-stage automatic thermocontrol system with an optical clear glass heating plate, ensures accurate, stable thermal control of specimens during observation under a microscope. The system includes a glass thermoplate (many models available), a temperature sensor, and a temperature controller. PRODUCT CODE: MAT STAGE WARMER PLUS The Stage Warmer Plus system is sized specifically for the user s stage warming needs and is adaptable to all brands of dissecting and stereoinverted microscopes. The stage warming deck is made of 6060 high tensile aluminum, which maintains a constant temperature (±0.2 C) between a setting range of 32 to 40 C. (Other ranges are available.) This easy-to-install system comes complete with instructions and necessary hardware. PRODUCT CODE: SWP BIOTHERM MICRO S 37 The BioTherm micro S 37 is a microscope warm stage specifically preset to 37 C. It is ideally suited to maintain stable specimen temperatures while examining or analyzing motile sperm samples under a microscope. It has a low profile that readily suits many microscopes, and minimizes heat loss or warming of the underlying microscope platform. There is automatic compensation for heating by light sources (or ambient heating). Heating is performed by direct current; there is no mechanical vibration, no electromagnetic interference and no induced magnetic fields. PRODUCT CODE: S37 LEC INSTRUMENTS LEC939 TEST TUBE WARMER The LEC 939 is a test tube/vial warmer capable of 0.25 C resolution. It is designed for holding specimens in the lab at a desired temperature above ambient conditions. Simple and affordable, it can be equipped with blocks for various test tube sizes. PRODUCT CODE: LEC B I O G E N I C S

33 BIOTHERM MSP The BioTherm MSP is a microscope warm stage with a selectable temperature range of +25ºC +50ºC (controllable temperatures start at 2ºC above ambient). The warm stage plate has a very low profile (less than 6mm) making it suitable for a wide range of microscopes. A large 22mm aperture stage is available for use with inverted and stereo microscopes. The BioTherm MSP can be used with Petrie dishes or slides. Heating is applied uniformly across the whole plate, eliminating any hot or cold spots on the plate. The heater uses direct current and is designed so that there is no net magnetic field in the plate. Hence there are no mechanical vibrations, there is no induced magnetization, and no electromagnetic interference to affect specimens or nearby instrumentation. BIOTHERM MSP SPECIFICATIONS Microscope plate dimensions: 112 x 112 x 5.6mm (L x W x H) Light aperture: 12 mm or 22 mm (diameter) Temperature controller dimensions: 180 x 130 x 56 mm (L x W x H) Cable from plate to controller: 75 cm Weight of plate and controller: 1130 g Surface temperature settings: +25ºC (or ambient + 2ºC) to 50ºC Temperature steps: 0.1ºC Surface temperature variation: < 0.2ºC (over entire plate surface) Warm-up time (from 10ºC to 32ºC): Less than 3 minutes Temperature overshoot: < 0.1ºC Short-term temperature fluctuations: < 0.02ºC Long-term temperature drift: < 0.3ºC per year Temperature variation with ambient: At 32ºC, less than 0.01ºC/ºC Power consumption: 32 watts Input requirements: Universal mains ( Volt, Hz) or 12V DC PRODUCT CODE: MSP B I O G E N I C S 35

34 LIFE SCIENCE TECHNOLOGY INSTRUMENTS STAGE AND SPECIMEN WARMERS LEC INSTRUMENTS LEC916 MICROSCOPE STAGE WARMERS Dedicated stages are designed to fit most brands of microscopes. The system includes a heated stage element to fit monocular, stereo, and inverted micro-scopes. The temperature controller has a heating pad on top and separate control circuits for the stage and heating pad. Each circuit gives accurate temperature control by use of a feedback system. A unique heating element is used to eliminate vibration of the stage, thus enabling a stable image at high magnification. PRODUCT CODE: LEC916 EXAMPLES OF DEDICATED STAGES FOR VARIOUS MICROSCOPES Bausch & Lomb Stereo zoom 7 Kyowa PLHL, 5DZ-PL Leica LABOVERT, DMIL, DM IRB Nikon Diaphot TMV 300/200, SMZ-2T, SMZ-10, SMZ-2B, SMZ-1B, SMZ-U, Labophot-2, SMZ-10A, Eclipse E400, E600, E800, TE300/200 Olympus IXC50/70, HB-SVR, SZH10, CH-2, IMT-2, HB-2, BX, CK2, CH30, CX40, BX50, SZX12, CHK-2 Orion BM-LUX-4, BM-LAB Wild M5, M8, M10, MZ8 Zeiss Standard 20, 25 LEC916 TECHNICAL SPECIFICATIONS Temperature range Ambient to 40 C Accuracy ±0.25 C Temperature indication 0.1 C Heating time of stage/pad 30 minutes nominal Size of warming pad 210 x 130mm Size of temperature controller 210 x 150 x 90mm Power 100 to 120, 200 to 240V, AC 50/60 Hz 60 VA 36 B I O G E N I C S

35 PORTABLE INCUBATORS BIOTHERM INC-12V/IRB-1 PORTABLE INCUBATORS/CELL CARRIERS The BioTherm INC-12V and IRB-1 are portable incubators designed for maintaining live cell cultures, gametes and tissues at appropriate metabolic temperatures, with less than 0.05 C temperature fluctuation or variation between specimens. Temperatures are maintained in the range C to C, selectable in steps of 0.5 C. The temperature is monitored and regulated continuously. The INC-12V is equipped with your choice of non-replaceable core. The IRB-1 is capable of interchangeable core geometries, and comes with two interchangeable cores of your choice. The incubators can maintain specimens at a constant temperature; temperature regulation is maintainable for periods exceeding 30 hours away from mains power. The incubators come complete with rechargeable batteries and built-in, world-wide-ready AC chargers. Tubes of various dimensions can be accommodated in the core. A range of standard tube cores is available. Other cores can be customized provided they are within specifications for the unit. The incubator is rugged and secure, and built to be transported by third parties. The long operating time makes it possible for live materials to be shipped nationally and internationally. PRODUCT CODE: INC-12V FEATURES AND SPECIFICATIONS Two internal 6V 4AH sealed lead acid batteries Universal mains input Internal mains battery charger Battery level indicator and battery warning LED External 12V connection Integral carrying/travel case Secure latching mechanism Fully removable lid Temperature range Temperature holding time Dimensions (WxDxH) Weight STANDARD INC-12V/IRB-1 CORES 32.5 C to 40 C (0.5 C increments) >30 hours (from internal batteries) 275 x 212 x 258mm ~6.0 kg (including batteries) V12 Capacity: 12 x 10ml tubes (15mm dia x 85mm) F6/6 Capacity: 6 x 14ml tubes (17mm dia x 75mm) 6 x 6ml tubes (12mm dia x 60mm) L14/14 Capacity: 14 x 1.8ml cryovials (13mm dia. x 34mm) 14 x auto-sample tubes (8.5mm dia. x 98mm) (continued) B I O G E N I C S 37

36 LIFE SCIENCE TECHNOLOGY INSTRUMENTS (continued) STANDARD INC-12V/IRB-1 CORES E10 Capacity: 10 x 14ml or 15ml tubes (17mm dia. x 96mm) G17 Capacity: 17 x 5ml or 6ml tubes (12mm dia. x 63mm) A12/1 Capacity: 12 x tubes (15mm dia. x 94mm) 1 x 14ml or 15ml tube (17mm dia. x 98mm) SC8/1 and removeable Straw Cassette (INC-12V only) Capacity: 8 x 5ml or 6ml tubes (12mm dia. x 63mm) 1 x 14ml or 15ml tube (17mm dia. x 97mm) 1 x cassette slot (141mm x 94mm x 11mm) 50 x 0.5ml or 100 x 0.25ml straws PORTABLE INCUBATORS LEC960 PORTABLE INCUBATOR/CELL CARRIER The LEC960 portable incubator maintains temperatures from +37 C to +39 C and has a temperature stability of ±0.25 C. This incubator accommodates various sizes of test tubes. It consists of interchangeable, temperature-controlled aluminum blocks with cavities for housing test tubes. Custom blocks are available. Operation is from an internal 12-volt battery, battery charger or a 12-volt vehicle battery. PRODUCT CODE: LEC B I O G E N I C S

37 CELL FUSION VOLTAIN EP-1 CELL FUSION/ACTIVATION SYSTEM The VOLTAIN EP-1 is a Cell Fusion/Activation system, which provides sinusoidal alignment signals and pulse fusion signals. Designed and manufactured by CryoLogic, the VOLTAIN EP-1 is reliable, simple to operate, and safe. PRODUCT CODE: EP1 FEATURES: Accurate & Stable Digital settings for pulse timing parameters overcome temperature and time drifts. Highly Rectangular Pulses Pulses have fast rise and fall times, and minimal droop. THE VOLTAIN EP-1 IS SUITABLE FOR A RANGE OF APPLICATIONS: Rapid cell alignment in highly stable sinusoidal fields Cell activation 70% activation of MII oocytes Cell fusion of very different to very similar cell sizes Cell permeation High Fidelity Alignment fields consist of very low distortion sinusoidal waves. High Stability Alignment frequency and amplitude is highly stable. Settings remain valid, allowing for easy repeatability. Wide Adjustment Range Alignment frequency and amplitude, and pulse timing parameters and amplitude, can be finely adjusted to suit a wide variety of cell types. Safe & Reliable The unit has full overload protection. Delayed operation for arming/alignment prevents false triggering. User Friendly The controls are easy to use and output parameters can be easily identified and adjusted. Operator interface has optional hands-free aligning and pulse triggering. (continued) B I O G E N I C S 39

38 LIFE SCIENCE TECHNOLOGY INSTRUMENTS CELL FUSION VOLTAIN EP-1 TECHNICAL SPECIFICATIONS ALIGNMENT FIELD Waveform Sinusoidal, distortion < 1% Alignment amplitude 0.0 to 25.0 volt peak (0 50Vpp, Vrms) Step size Continuous Alignment frequency 10KHz to 1200KHz Step size Continuous Alignment field current limit 200mA FUSION / ACTIVATION FIELD Pulse shape Square Rise / fall time 0.05µs typical Droop < 2% for 150W load Pulse amplitude =OV to +500 Volt Step size Continuous Pulse current limit 10A typical Pulse duration 1 to 999µs Step size 1µs Tolerance +0.15µs typical Repeat pulse delay 10ms to 9.99s Step size 10ms Tolerance +2µs max Repeat pulse count 0 99 OTHER Displays Selectors arm/fire Alignment voltage/frequency Pulse voltage Pulse timing Pulse timing parameters LED Momentary buttons or foot switches Potentiometers (10 turn) Potentiometer (10 turn) Quartz crystal Decade switches GENERAL Mains supply voltage 120 / 240Vac ±20% Power consumption 25VA Operating temperature range 0 to +40 C Dimensions (WxDxH) 14 x 35 x 21cm Weight 5 kg 40 B I O G E N I C S

39 ALARMS CRYOLERT LN 2 LEVEL SENSING TANK ALARMS Biogenics developed CryoLert in response to demand for an economical, true level-reading tank alarm systems. Research shows that to provide true protection to valuable biological specimens, an alarm should monitor cryogenic refrigerant level rather than tank temperature. The CryoLert continuously monitors actual liquid nitrogen level and allows the practitioner to specify an alarm point based on canister exposure out of LN 2. CryoLert liquid nitrogen alarm alerts the practitioner both audibly and visibly when LN 2 levels fall below a preset point of safety or if the sensor or power has failed. LN 2 levels routinely decrease due to slow evaporation or deteriorated vessel vacuums. Often, these levels drop rapidly without warning. The CryoLert was engineered with many features, including power failure indicator with auto reset, relay contacts for an external alarm, and an LN 2 fill timer which disables alarm outputs thus preventing false alarms while filling the vessel or manipulating specimens. CRYOLERT FEATURES: Audible 15-minute warning if power to the unit is interrupted Separate green and red LEDs for LN 2 high or low conditions respectively 100% indication of all possible faulty sensor conditions Resettable Fill Timeout button providing 5 minutes of interruption for filling or extraction Relay output for an external alarm or automatic telephone dialer system* 12-volt output to power an external alarm or remote device Silence button to stop power failure warning signal Automatic reset after power is restored Safe, low DC voltage operation PRODUCT CODE: BGC * Optional available through Biogenics 6-CHANNEL CRYOLERT FEATURES: Monitors up to six independent LN 2 refrigerators Microprocessor-controlled, non-volatile firmware Instant visual status for all refrigerators monitored Large, back-lit liquid crystal display Complete self-diagnostics with faulty sensor and power failure notification Output for autodialer The most advanced, lowest-cost system on the market BENEFITS: A cost-effective solution for when three or more refrigerators need monitoring Centralized monitoring for peace of mind 24 hour, 365 day monitoring of liquid levels PRODUCT CODE: CL6C Specify number of probes when ordering PRODUCT CODE: 6CP B I O G E N I C S 41

40 LIFE SCIENCE TECHNOLOGY INSTRUMENTS MICROSCOPES WESCO 601 MICROSCOPE This 6:1 binocular zoom stereo scope is perfect for use in embryology. The 601 microscope offers superior optics at an attractive price. PRODUCT CODE: W SPECIFICATIONS Magnification 15x 94.5x Working distance 100mm Field of view 15.5mm 2.5mm WESCO 701 ZOOM STEREO MICROSCOPE The 701 Zoom Stereo Microscope is designed for superior optical and mechanical performance. With its wide 7:1 zoom ratio and choice of accessories, the 701 is an extremely versatile instrument. Many configurations are available, making the 701 the clear choice for industrial or bio-medical use. PRODUCT CODE: W SPECIFICATIONS Working distance 100mm Interpupilary distance 55-75mm Diopter adjustment ±5 Focus range 135mm Observation body 45 inclined, 360 rotatable Stage 95mm stage plate 42 B I O G E N I C S

41 MICROSCOPES WESCO CXRIII COMPOUND MICROSCOPE WESCO CXRIII is a laboratory microscope system for high-end application, with a modern design incorporating the latest optical and mechanical advancements. Extra clarity and contrast is provided through high performance infinity corrected IP optical system. WESCO CXRIII offers great performance features standard, making it a clear choice for advanced laboratory and research applications. PRODUCT CODE: WCXR LABELING SYSTEMS BRADY TLS2200 LABELING SYSTEM The TLS2200 is an efficient, cost-effective, and time-saving solution to the challenge of labeling biological specimens for cryostorage. This unique handheld instrument generates labels with specially formulated adhesive for straw ID rods, cryovials, test tubes, and other labware. The user has control of the type size, number of lines, orientation, and virtually every parameter of the print format. Bar coding is also supported by the TLS2200. The TLS2200 comes standard with attaché-style carry case, rechargeable battery and AC adapter (specify USA or international version), sample labels, Identilab software, protective cover, cleaning kit, and comprehensive instructions. PRODUCT CODE: TLS BRADY TLS PC LINK LABELING SYSTEM The TLS PC Link is a rugged, small and lightweight desktop/portable thermal labeling system for identifying biological specimens for cryostorage. The printer provides capabilities such as thermal transfer printing, bar coding, and multiple material configurations. The TLS PC Link printer uses all of the available TLS2200 materials and ribbons, and also runs the latest bulk rolls of material as well. The included Windows drivers allow the unit to print labels from both LabelMark (included with TLS PC Link) and Codesoft software, or any Windows software application. In addition, the printer has its own printer control language, allowing the unit to be integrated with a piece of equipment and precisely print labels whenever and wherever they are needed. The firmware is upgradeable through the mini-din serial jack. The unit operates off AC power or the included rechargeable battery. PRODUCT CODE: TLSPC B I O G E N I C S 43

42 LABORATORY CONSUMABLES BIOGENICS 6-WELL VENTED CULTURE DISH Biogenics 6-well vented culture dish reflects an innovative rethinking of the specific requirements of the assisted reproductive laboratory. The features of this multi-welled dish were designed in response to the demands associated with handling, sorting and culturing spherical cell structures and other sensitive biological specimens. Unique to the 6-well vented culture dish are its radiused corners in each well which allow specimens to roll into the center of each well and avoid sticking into corners because the wells have no corners. The vented lid allows for two positions indicated by molded keyways positioned 90º apart. The open position elevates the lid uniformly by 1mm allowing for gas exchange and prevention of contamination when placed inside an incubator, whereas the closed position allows for secure transport across the lab. Standard-welled dish Biogenics 6-well vented culture dish The center well s patent-pending convex/concave geometry creates an annular canyon where specimens may be sorted prior to culturing. Alternatively, this well is useful for humidification. Tight tolerance between the skirt edge and the well bottoms ensure good thermal transfer when the dish is place upon a heated stage. Ribs on the dish lid interface with a mechanical interference fit on the skirt of another culture dish, allowing the dishes to be stacked vertically with reduced risk of spillage during transport or incubation. Each well is numbered for the purpose of tracking individual specimens. The additional two wells (compared to a 4-well dish) result in better economy and value. The dishes are manufactured from 100% virgin, crystalline medical grade polystyrene. All production lots of the dish are subjected to a rigorous 72-hour optical polish process, which results in a clarity surpassing all competitive products. After sterilization by gamma irradiation they undergo a 96-hour mouse embryo assay test wherein test results are typically % post culture. Dishes are supplied in case lots of 120 dishes. Each dish is individually wrapped and sterile, thus reducing the potential waste of unused dishes. PRODUCT CODE: B I O G E N I C S

43 BRADY AC Adapter Battery Charger Battery Pack Cleaning Kit LABELS WHT-TRANS Straws YEL-TRANS Straws PTL Vials R62-10 Ink Cartridge PRODUCT CODE: BRAC PRODUCT CODE: BRBC PRODUCT CODE: BRBP PRODUCT CODE: BRCK PRODUCT CODE: B427W PRODUCT CODE: B427Y PRODUCT CODE: 21427V PRODUCT CODE: BRIC MISCELLANEOUS LABORATORY CONSUMABLES DISHES Dish 4-Well Nunclon, 4/pkg, 120/case Dish 35 x 10mm ESP, 20/pkg, 500/case Dish 35 x 10 Nunclon, 10/pkg, 500/case Dish 60 x 15mm ESP, 20/pkg, 500/case Tissue Culture Plate ESP, 4/pkg, 100/case Well Vented Culture Dish, 1/pkg, 120/case Well Vented Culture Dish, 1/pkg, 120/case Square Dish with Grid ESP, 4/pkg, 100/case SYRINGES Syringe Air-Tite, all plastic, 1cc, 100/box Syringe Air-Tite, all plastic, 3cc, 100/box Syringe Air-Tite, all plastic, 5cc, 100/box Syringe Air-Tite, all plastic, 10cc, 100/box Syringe Air-Tite, all plastic, 20cc, 100/box Syringe Air-Tite, all plastic, 30cc, 100/box Syringe Air-Tite, all plastic, 50cc, 100/box Syringe Monoject, 1cc T.B., 100/box Needle Monoject, 18 Ga x 1", 100/box Needle Monoject, 18 Ga x 1 1/2", 100/box Needle Monoject, 20 Ga x 1", 100/box Needle Monoject, 20 Ga x 1 1/2", 100/box Needle Monoject, 22 Ga x 1", 100/box Syringe Filter Acrodisc #4192, 0.22um, 25mm membrane, 50/box Syringe Filter Acrodisc #4652, 0.22um, 32mm membrane, 50/box Syringe Filter Acrodisc #4184, 0.45um, 25mm membrane, 50/box B I O G E N I C S 45