Arkansas Analytical, Inc.

Transcription

1 Arkansas Analytical, Inc. Toxicity Test Results ENTERGY ARKANSAS, INC. WHITE BLUFF Outfall 00 October, 00 AFIN# -000 Pimephales promelas, Acute Toxicity, EPA-8-R-0-0, October 00 Daphnia pitlex, Acute Toxicity, EPA-8-R-0-0, October 00 Prepared for: Mr. Barry Snow Entergy Arkansas, Inc. White Bluff Road Redfield, AR 7 Prepared by: Arkansas Analytical, Inc. 70-0, Bldg, Suite Little Rock, Arkansas 709 Lab Number Kl 008 Monday, November 08, 00

2 Introduction This report contains test results for the toxicity testing of Entergy Arkansas, Inc.- White Bluff Plant. The NPDES permit number is AR006. The permit requires acute biomonitoring testing bi-annually for Daphnia pulex and Pimephales promelas. The test results in this report represent the testing for the fourth quarter of 00. The plant is located in Section 0, Township South, Range 0 West in Jefferson County, Arkansas. The discharge is to receiving waters named the Arkansas River in Segment C of the Arkansas River Basin. Plant Operations To be provided by permittee. Toxicity testing, Pimephales promelas and, Daphnia pulex, Entergy-White Bluff Plant, Lab # Kl 008 Page

3 Source of Effluent and Dilution Water Samples were collected at the final discharge of Outfall 00, which is located at the cooling tower blowdown pipeline (down line of cooling tower sump). The samples were -hour flow-weighted composites. Mean daily discharge is to be provided by permittee. Effluent samples were collected as follows: Sample Collection: Sample A: Date, Time Started 0--0, 0 Date, Time Ended 0-6-0,00 Date, Time Received 0-6-0, 60 Storage Temperature Co Sample B: 0-6-0, , , The receiving water samples were grab samples collected approximately 00 yards upstream from the final discharge of Outfall 00. Stream flow at the time of sampling and 7Q0 information is to be provided by the permittee. Receiving water samples were collected as follows: Sample Collection: Receiving Water: Date, Time Collected 0-6-0, Date, Time Received 0-6-0, 60 Storage Temperature ( C) Chain of custody documentation and flow documentation are located in Appendix A. Both effluent samples and receiving water samples were analyzed for ph, hardness, total alkalinity, and conductivity. Results are provided in Appendix B. Dilution Series Five dilutions in addition to a control (0% effluent) were used in the toxicity tests. The dilutions, which were made with receiving water, were 9%, %, %, %, and 9%. The low-flow effluent concentration (critical dilution) was defined as % effluent. Toxicity testing, Pimephales promelas and Daphnia pulex. En tergy-white Bluff Planl, Lab # K.008 Page

4 Test Methods The analyses performed were 8 Hour Static Renewal Acute Toxicity Tests using the fathead minnow, Pimephales promelas, and the daphnid, Daphnia pulex. The tests were conducted according to EPA-8-R-0-0, October 00. The endpoint of the test is death, established by either no movement or no reaction to gentle prodding. Raw data is provided in Appendix B. Statistics are provided in Appendix C. Deviation from method: Date, Time Started Date, Time Ended Type and volume of test chambers: Volume of solution per chamber: Number of organisms per chamber: Number of replicates: Feeding frequency and amount: Acclimation temperature of organisms: Test temperature: Daphnia Pulex None 0-7-0, , 0 I -ounce plastic cups milliliters 8 None degrees Centigrade degrees Centigrade Pimephales promelas None 0-7-0, , ml plastic cups 0 milliliters 0 None degrees Centigrade degrees Centigrade Test Organisms Scientific name: Age: Source: Diseases and treatment Daphnia pulex < hours old In house culture None Pimephales promelas 6 days old Aquatox, Inc. None Organism History is provided in Appendix D. Toxicily testing, Pimephales promelas and Daphnia ptilex, Entergy-White Bluff Plant, Lab # K008 Page

5 Quality Assurance Test Acceptability Control Criteria Greater than or equal to 90% survival TEST ACCEPTANCE CRITERIA for Daphnia pulex Results % Pass X Fail TEST ACCEPTANCE CRITERIA for Pimephales promelas Control Criteria Greater than or equal to 90% survival Results 98% Pass X Fail Reference Toxicant The reference toxicant used was Potassium Chloride prepared in-house. The tests were performed using moderately hard synthetic as dilution water. The results of the reference toxicant were: REFERENCE TOXICANT Daphnia pulex Pimephales promelas Date performed: NOEC Survival: LOEC Survival: LC0: 9/-7/0 00 ppm KC loooppmkcl 7ppmKCl Date performed: NOEC Survival: LOEC Survival: LC0: 9/-7/0 00 ppm KC 0 ppm KCI 07 ppm KCI Quality Assurance charts arc provided in Appendix E. Toxicily testing, Pimephales promelas and Daphnia pulex, Entergy-While Bluff Plant, Lab # K008 Page

6 SUMMARY FORMS FOR ACUTE BIOMONITORING FATHEAD MINNOW LARVAE SURVIVAL Pimephales promelas PERMITTEE: Entergy Arkansas, Inc. -White Bluff Plant NPDES #: AR006 Sample Collection: Sample A: Sample B: Date, Time Started 0--0, , 0 Date, Time Ended 0-6-0, , 0 Test initiated (date, time): 0-7-0, 0 Test terminated (date, time): 0-9-0, 00 Dilution water used: Receiving Water DATA TABLE FOR FATHEAD MINNOW SURVIVAL Percent Survival in Replicate Chambers Mean Percent Survival Effluent Cone % 0% 9% % % % 9% A B c 90 D E hours 98 8 hours cv% Coefficient of Variation = standard deviation / mean * Toxicity testing, Pimephales promelas and Daphnia pulex, Entergy-White Bluff Plant, Lab # K] 008 Page 6

7 SUMMARY REPORTING FORMS FOR ACUTE BIOMONITORING FATHEAD MINNOW LARVAE SURVIVAL Pimephales promelas Permittee: Entergy Arkansas, Inc. -White Bluff Plant NPDES #: AR006. Dunnett's procedure or Steel's Many-One Rank Test as appropriate: Is the mean survival at 8 hours significantly different (p=0.0) than the control survival for: a) LOW FLOW OR CRITICAL DILUTION, (%) YES NO_X. If you answered NO to la. enter [0] otherwise enter [] parameter #TEM6C: 0. Enter percent effluent corresponding to the parameter below: NOEC for Pimephales promelas (parameter #TOM6C) = 9 % effluent Coefficient of variation (parameter #TQM6C) =.6 % Toxicity testing, Pimephales promelas and Daphnia pulex, Enlergy- While Bluff Plant, Lab # Kl 008 Page 7

8 SUMMARY FORMS FOR ACUTE BIOMONITORING Daphniapulex SURVIVAL RESULTS Permittee: Entergy Arkansas, Inc. White Bluff Plant NPDES#: AR006 Sample Collection: Sample A: Sample B: Dale, Time Started 0--0, ,0 Date, Time Ended 0-6-0, , 0 Test initiated (date, time): 0-7-0, 0 Test terminated (date, time): 0-9-0, 0 Dilution water used: Receiving Water DATA TABLE FOR Daplmia pulex SURVIVAL Percent Survival in Replicate Chambers Effluent Cone % 0% 9% % % % 9% A B 87. c 87. D 87. E Mean Percent Survival hours 8 hours cv% Coefficient of Variation = standard deviation/ mean * Toxicity testing, Pimepliales promclas and Daplmia pulex. Entergy-White Bluff Plant, Lab # Kl 008 Page 8

9 SUMMARY REPORTING FORMS FOR ACUTE BIOMONITORING Daphniapulex SURVIVAL Permittee: Entergy Arkansas, Inc. -White Bluff Plant NPDES #: AR006. Dimnelt's procedure or Steel's Many-One Rank Test as appropriate: Is the mean survival at 8 hours significantly different (p=0.0) than the control survival for: a) LOW FLOW OR CRITICAL DILUTION, (%) YES NO_JC. If you answered NO to la. enter [0] otherwise enter [], parameter #TEMD: 0_. Enter percent effluent corresponding to the parameter below: NOEC for Daphnia pulex (parameter #TOMD) = 9 % effluent Coefficient of variation (parameter #TQMD) =.7 % Toxicity testing, Pimephales pramelas and Daphnia pulex, Entergy-White BlulT Plant, Lab # Kl 008 Page 9

10 Summary of Results Entergy Arkansas, Inc. -- White Bluff Plant NOEC / LOEC Survival LC0 Daphnia pulex 9%/NA NA PASS NA NOEC/ LOEC survival Pimephales promelas 9% / NA LC0 NA PASS NA Conclusion EPA-8-R-0-0, October 00, Pimephales promelas The permit issued to the Entergy Arkansas, Lie.- White Bluff Plant, AR006, specifies that the critical dilution is % effluent. The effluent samples did not exhibit acute toxicity at the critical dilution, and, as such, the effluent samples passed the test. Therefore, there is no further action to be taken. EPA-8-R-0-0, October 00, Daphnia pulex The permit issued to the Entergy Arkansas, Inc.- White Bluff Plant, AR006, specifies that the critical dilution is % effluent. The effluent samples did not exhibit acute toxicity at the critical dilution, and, as such, the effluent samples passed the test. Therefore, there is no further action to be taken. Biomonitoring Analysts: KennejirPigue Toxicity testing, Pimeplmles promelas and Daphnia pttlex. Entergy-While Bluff Plant, Lab # K.008 Page 0

11 APPENDIX A Chain of Custody Forms

12 70 Interstate 0, Bldg., Ste. Little Rock, AR 709 PHONE: 0-- FAX: CHAIN OF CUSTODY RECORD CLIENT INFORMATION j Project Description Turnaround Time Preservation Codes: Entergy Arkansas, White Bluff Plant 00 White Bluff Rd. Redfield,AR 7 Attn: Barry Snow White Bluff Biomonitoring Dmonitoring Reporting Information Telephone: Fax: BSNOW@entergy.co'm Hour 8 Hour I. Cool, Degrees Centigrade. Sulfuric Acid (H,SO,), ph <. Tliiosulfatc for Dedilorinatioti. Hydrochloric Acid(llCI) 7 Hour. Nitric Acid (HNO,),pH< 6. Sodium Hydroxide (NaOH), pi) f Routine TEST PARAMETERS Bottle Tjjpe Code Preservative Code: G =-- Ghiss: P = Wasnc Bottle Type: P V = Septum. A - Amher Sampler(s) Signature Field Number SAMPLE COLLECTION Date/s Time/s Sampler(s) Printed Grab Comp Number of Bottles Sample Matrix 0^~>*^ SAMPLE IDENTIFICATION/ DESCRIPTION m jd o Arkansas Analytical Work Order Number: 'oas */ / ;>' W^L0O X Water Outfall X L. Relinquished bv: (Signature) Date/Time. Received bv: (Slanaturel ^^ SAMPLE CONDITION UPON RECEIPT IN LAB REMARKS / SAMPLE COMMENTS /X^X*^ ^ - ^' ^"^ \ CUSTODY SEALS: V Yes No ^^^, CONTAINERS CORRECT: f Yes No. COC/LABELS AGREE: Yes No s&\s*u /Vu> S<^t <r*+ &Xfr *+/ s*^6tt>. -T&**? ^ irc^ > / s. Relinaulshed bv: (Slanatur»K^^ Date/Time ft&acelved bv lab: (Slanature). PRESERVATION CONFIRMED: Yes No ^ ^ >- jlsl'lssy? ' RECEIVED ON ICE; -J-Ye s / 6. TEMPERATURE ON RECEIPT: / FOR COMPLETION BY LAB ONLY Y No

13 70 Interstate 0, Bldg., Ste. Little Rock, AR 709 PHONE: 0^- FAX: CHAIN OF CUSTODY RECORD CLIENT INFORMATION j Project Description Entergy Arkansas, White Bluff Plant White Bluff Rd. Redfield, AR 7 Attn: Barry Snow White Bluff Biomonitoring Dmonitoring Reporting Information Telephone: Fax: BSNOW@entergy.com Turnaround Time Hour 8 Hour 7 Hour. Cool, Degrees Centigrade. Sulfuric Acid (II,SO,, ph <. Nitric Acid (HMO,), pll < Preservation Codes:. Thiosulfalt fur Dcclilorinatiun. Hydrochloric Acid(HCI) 6. Sodium Hydroxide (NaOH), ph = I" Routine TEST PARAMETERS llotllo ' y\uf Onlf Preservative Code: G =-- Glasx!' hiistk- Bottle Type: P V = Septum. A Amber Samplers) Signature /T\V<^A Sampler(s) Printed V C*F'\*&*C Arkansas Analytical Work Order Number: Field Number SAMPLE COLLECTION Date/s Time/s Grab Comp Number ol Bottles Sample Matrix SAMPLE IDENTIFICATION/ DESCRIPTION CD Si O V^/&-Cl7//e> mo-\-s*o X Water Outfall X VL/<?/tfe**tf. Relinaulshed bv: (Slanature) ^e^.. Relinaulshed bv: (Sianature) ^^ Daterrime Wfalto \^6 Date/Time. Received bv: (Slanaturel SAMPLE CONDITION UPON RECEIPT IN LAB """^ ^^ ^^". CUSTODY SEALS:. CONTAINERS CORRECT:. COC/LABELS AGREE: y Yes ^ux <T Received bv lab: (SJonnture). PRESERVATION CONFIRMED: Yes /"-Urn i d>l - RECEIVED ONICE: ^- Yes Cfv^7 I 6- TEMPERATUR EON RECEIPT: \^'C/ J FOR COMPLETION BY LAB ONLY Yes Yes No No No NO No REMARKS / SAMPLE COMMENTS

14 APPENDIX B Physical, Chemical, and Raw Data for Fathead Minnow and Daphniapulex

15 Bio monitoring Report \cute /8 Hour Static Test Lab Number: Client: Date/ Time Started: Date/ Time Ended: Test Organism: Age of Organism: Source of Organism: Dilution Water: Cone: Rep # Live Organisms PH Dissolved 0 mg/l Alkalinity mg/l Hardness mg/l Conductivity T Res Cl mq/l #-' Ohr hr 8 hr hr 8 hr hr 8 hr hr 8 hr hr 8hr hr 8 hr hr 8 hr O- A lu l?---f a zx D U A 0 D D la A -> ft a A 0 b- D A a *a SO 0 E D L. ZS C. ' *^ ffi. A a IPa. 9. M 7TJ -7 - j&- 007

16 Biomonitorinq Report \cute /8 Hour Static Test Lab Number: Client: Date/Time Started: jojzlfto Date/ Time Ended: Test Organism: Age of Organism: Source of Organism:-? /, Dilution Water: Cone: o PI t"^ o L [( -^ ' Kep #." A B C D E A B C D E A B C D E A B C D E A R C n F A R C 0 F # Live Organisms Ohr 8 hr r^ -^ <? I S j J ^ g / - v * j^ ^ hr e? p e i &$ej J V V X I T i i s7 X i8 7 0 v I I ph hr 8 hr ^A m 7,^7 IS "? % i 7V i 7 s -p^) ^tfl ~7 V y/ S H L ~-?o\ ' I? f -7$ ~J \ ~f$ ^. Dissolved 0 mg/l Alkalinity mg/l hr 8 hr hr 8 hr ""7.*T~ *? - -7 ~~?^7 z J 7^? ~7(* ">^? ^ ^ i. ^ /^ ^j? -7-7 i i^y 7V 77 "&!? "/7 ^ ^> Hardness mg/l hr 8hr.? L. - d }(ogd \^./vsl Conductivity hr 8 hr vt'77,"' ^/7A d?yt//) T Res Cl mq/l hr 8 hr ^o^r"- r( <>^t,' c^-fite^

17 APPENDIX C Fathead Minnow and Daphniapulex Statistics

18 AA # K008, Pimphales promelas, 8 HR ACUTE, 0-7- File: Z:\TOXSTAT\WBLUFF\FH. Transform: ARC SINE(SQUARE ROOT(Y)) Shapiro - Wilk's test for normality D = W Critical W (P Critical W (P 0.0) (n = 0 0.0) (n = 0 = 0.97 = Data FAIL normality test. Try another transformation. Warning - The first three homogeneity tests are sensitive to non-normal data and should not be performed. AA # K008, Pimphales promelas, 8 HR ACUTE, 0-7- File: Z:\TOXSTAT\WBLUFF\FH. Transform: ARC SINE(SQUARE ROOT(Y) Hartley's test for homogeneity of variance Bartlett's test for homogeneity of variance These two tests can not be performed because at least one group has zero variance. Data FAIL to meet homogeneity of variance assumption. Additional transformations are useless. TITLE: AA # K008, Pimphales promelas, 8 HR ACUTE, 0-7- FILE: Z:\TOXSTAT\WBLUFF\FH. TRANSFORM: ARC SINE(SQUARE ROOT{Y)) NUMBER OF GROUPS: GRP IDENTIFICATION REP VALUE TRANS VALUE g, " 0, " ft, " o, " o, "o CONTROL CONTROL CONTROL CONTROL CONTROL

19 o, "o a. "o g, * a. is a. " &, " a,, "8 g, Ti a, IS g. "a g, " g, " a. "o 0, ft. "o a. T> a. TS a, * o, " AA # K008, Pimphales promelas, 8 HR ACUTE, 0-7- File: Z:\TOXSTAT\WBLUFF\FH. Transform: ARC SINE(SQUARE ROOT(Y)) STEEL'S MANY-ONE RANK TEST Ho:Control<Treatment GROUP IDENTIFICATION TRANSFORMED MEAN RANK SUM CRIT. VALUE df SIG o, "O a. "8 a, * a, " g, " CONTROL Critical values use k =, are tailed, and alpha = 0.0

20 AA # K008, DAPHNIA PULEX, 8 HR ACUTE, File: Z:/toxstat/wbluff\DP. Transform: ARC SINE(SQUARE ROOT{Y) Shapiro - Wilk's test for normality D = 0.0 W = 0.7 Critical W (P Critical W (P 0.0) (n = 0 0.0) (n = 0 = 0.97 = Data FAIL normality test. Try another transformation. Warning - The first three homogeneity tests are sensitive to non-normal data and should not be performed. AA # K008, DAPHNIA PULEX, 8 HR ACUTE, File: Z:/toxstat/wbluff\DP. Transform: ARC SINE(SQUARE ROOT(Y) Hartley's test for homogeneity of variance Bartlett's test for homogeneity of variance These two tests can not be performed because at least one group has zero variance. Data FAIL to meet homogeneity of variance assumption. Additional transformations are useless. TITLE: FILE: TRANSFORM: AA # K008, DAPHNIA PULEX, 8 HR ACUTE, Z:/toxstat/wbluff\DP. ARC SINE(SQUARE ROOT(Y)) NUMBER OF GROUPS: 6 GRP IDENTIFICATION REP VALUE TRANS VALUE g, " o, "o a, * Q, "0 a, " CONTROL CONTROL CONTROL CONTROL CONTROL

21 , " g, "o g. * a. TS o, * a. T> Q, * &. "o Q. T O, "o a, "o g, T a. o, "6 g, " a, 'V <L Ti o, "6 9- IS a., AA # K008, DAPHNIA PULEX, 8 HR ACUTE, File: Z:/toxstat/wbluff\DP. Transform: ARC SINE(SQUARE ROOT(Y)) STEEL'S MANY-ONE RANK TEST Ho:Control<Treatment GROUP IDENTIFICATION TRANSFORMED MEAN RANK SUM GRIT. VALUE df SIG Q, Q, " g, " o, * 0. * CONTROL Critical values use k =, are tailed, and alpha = 0.0

22 APPENDIX D Organism History

23 AQUATOX, INC. 6 TWIN POINTS ROAD HOT SPRINGS, ARKANSAS TEST ORGANISM HISTORY DATE SHIPPED /&'<?7'/O CLIENT Purchase Order #: SPECIES: Cjpjmephales promefos Mysidopsis bahia Cyprinodon variegates Quantity Shipped: >. Age: Brood Stock Source: Culture Water: Artificial Salts Artificial Salts Hardness (Mg/ CaCO) Salinity (ppt)_ Dissolved Oxygen (Mg/): Feeding: Comments: Shipped Via: Packaged By: Federal Express UPS Overnight Bill Hall Primers 7I

24 I. Organism History Aquatic Research Organisms DATA SHEET Species: O Source: Lab reared Hatcher/ reared Field collected. Hatch date /< Receipt date Lot number O( ao QfbVP Strain Brood Origination T II. Water Quality Temperature ^ C Salinity ppt DO ph / Hardness ppm III. Culture Conditions System: Diet: Fiake Food Brine Shrimp^ Prophylactic Treatments:. r^ Comments: A-\l ST\\ r&j? Phytoplankton, Rotifers Other Trout Chow_ /cr IV, Shipping Information Client: ff of Oranism

25 APPENDIX E Quality Assurance Charts

26 ARKANSAS ANALYTICAL, INC. FATHEAD MINNOW NOEC QUALITY ASSURANCE 8 HOUR ACUTE *-uu _ + NOEC # x *w ^^ - " H <$?> ^? <$? <$? ^ N^ \ \ NQ»$> ^ *J A L LCL. J Date Test Performed ARKANSAS ANALYTICAL, INC FATHEAD MINNOWLC0 QUALITY ASSURANCE 8 HOUR ACUTE 0, O 000 C ^- c & to"- 0 '

27 ARKANSAS ANALYTICAL, INC DAPHNIA PULEXNOEC QUALITY ASSURANCE 8 HOUR ACUTE Reference Toxicant, KG E a , 00 ' Date Test Performed ARKANSAS ANALYTICAL, INC. DAPHNIA PULEX LC0 QUALITY ASSURANCE 8 HOUR ACUTE