Cultivo CO 2. Incubator. Underwriter Laboratories Listed

Transcription

1 Cultivo CO 2 Incubator Underwriter Laboratories Listed

2 Your work. Your cultures. Your way. How do you define optimal? You use CO2 incubators to provide optimal atmospheric conditions to protect, grow and maintain cell and tissue cultures. But how do you define optimal? Optimal is never a one-size-fits-all condition. It s contextual. It s driven by the research or the cell line. But more importantly it should be driven by you. Providing optimal conditions means you control the parameters of the particular environment in which your specific cells will thrive, or be challenged. You define the variables, not the incubator.

3 With full, finely-tuned control over three variables (CO2, temperature and relative humidity), you can create more lifelike conditions for your cultures to thrive, and improve the validity of your results. Accurate, precise and stable environmental conditions make your results more reliable. Find out how cells respond in challenging conditions. Work with sensitive cell lines. With Cultivo, you can provide the unique conditions required to explore more research questions than ever before. Whether growing a few plates of microorganisms for a small laboratory or managing a largescale tissue culturing operation, your success hinges on how well your incubator prevents contamination. Cultivo draws on Baker s deep knowledge of air containment and contamination control technologies to provide unparalleled protection of your work. User-driven features help increase throughput by solving common problems and eliminating tedious water pan changes (Ultra and Ultra Plus models). With one of the largest usable workspaces among incubators of the same type and size, productivity is improved. Economical and user-defined configurations with plenty of options and accessories that allow you to determine the level of control and protection your research requires.

4 Protect Cultures from Contamination Baker Clean Air Technology With more than 60 years of innovation in contamination control technology, at Baker, we know how to prevent and control contamination. The design of Cultivo draws on this extensive experience to protect cultures against the threat of accidental contamination from a wide variety of sources due to everyday activities. Vertical, uni-directional downward airflow delivers better-than ISO Class 4 (Class 10) air to the chamber, keeping cultures safe from contaminants by sweeping them away and trapping them in the full-face HEPA filter. This uniform downflow also helps reduce variations in temperature throughout the chamber, without drying out sensitive cultures. Large, full-face HEPA filter captures contaminants introduced into the chamber after door opens. Cultivo takes less than 60 seconds to provide better-than-iso Class 4 (Class 100) air the fastest filtering rate of any incubator model. Uniform downward airflow inside Cultivo air flows down to the chamber bottom, under the false floor, passing over the humidity source. The fan pulls air tup behind the rear baffle to the top of the unit, where it passes through a full-face HEPA filter, delivering clean, better-than-iso Class 4 (Class 10) air. Crevice-free interior with coved corners is easy to clean and won t harbor potential contaminants. Superior condensation control virtually eliminates wet spots where contaminants can grow and spread. Fogless interior door with heated frame provides a crystal-clear view to every shelf, reducing the need for door openings, when contaminants are most likely to enter the chamber. Ultrasonic humidity delivery system (optional) eliminates the water pan altogether, along with the risk of contaminants that typically go with it. copper interior components stop contaminants in their tracks, whether they result from spills or recirculation into the system from the water pan. Screen shot from Cultivo air filtration test video. A difference you can see watch how Cultivo s air filtration system performs against a leading competitor!

5 Unmatched Control and Accuracy Cultivo provides users with precise control, as well as industry-leading stability, uniformity and recovery rates. Even with a full load, cultures grow consistently from shelf to shelf, for dependably high-quality, robust cells on every plate. InteliCELL proprietary PID control algorithm provides pinpoint, user-defined control over all three parameters (temperature, CO2 and relative humidity), with recovery not seen in other CO2 incubators. Uniform vertical downflow air circulation enhances the uniformity and stability of conditions inside the chamber without disturbing or dehydrating cultures. Temp Recovery CO2 Recovery 37.5 RH Recovery min min (Evaporative) Time (Minutes) 5 10 Time (Minutes) 15 For 20% faster humidity recovery, select Cultivo Ultra or Ultra Plus with ultrasonic humidification. Relative Humidity (%) 4.5 CO2 (%) 6 min Temperature ( C) Time (Minutes) InteliCELL Meet your incubator s brain InteliCELL is the sophisticated, proprietary PID control algorithm that allows you to define and sustain the optimal conditions for your work. It s beyond smart technology it s the intelligence that drives Cultivo s unparalleled control, stability and uniformity. By accounting for factors that other technologies leave as unknown, InteliCELL consistently maintains the setpoints that you define, giving you ultimate control with minimal effort. The core of InteliCELL s design is a closed feedback loop that works just like your nervous system, sensing and responding to changing conditions in a controlled way. Highly precise temperature, CO2 and humidity sensors continuously sample interior conditions, feeding data back to the control algorithm. InteliCELL evaluates this data and instantly responds by making small, controlled adjustments to maintain set points. This feedback loop allows Cultivo to provide consistent conditions even across different ambient laboratory environments. InteliCELL is also self-tuning and adaptable to changing laboratory environments and usage rates. With all that it does for your cultures, InteliCELL truly is the brain behind Cultivo.

6 Active Humidity and Condensation Control No gimmicks. Most CO2 incubators only allow users to control two variables (temperature and CO2) and employ clever gimmicks for passively managing condensation formation. True active humidity and condensation control gives users the ability to define relative humidity in a simple, two-touch process. At Baker, we know that temperature, CO2 and relative humidity (RH) are each vital to providing optimal environmental conditions for cell growth. Our unique PID control algorithm, InteliCELL, combined with highly precise sensor technology, allows Cultivo to actively control humidity and prevent condensation from forming, even at higher levels of RH (>90%). To learn more about how Baker delivers active humidity and condensation control under typical (up to 90%) and higher (90%-95%) levels of humidity, download our white paper. Learn more - scan the code to get the relative humidity white paper! Uniformity and Consistency Eliminate the Sweet Spot Because Cultivo provides unmatched uniformity of interior conditions, there s no more competition for the sweet spot in the incubator, where the healthiest cell growth tends to occur. Growth Rate Assessment Shelf Positioning A B C Shelf 2 Shelf 3 Bottom 1.5 x 107 Cells / ml / 24 hours Top S. epidermidis 1.2 x 107 S. marcescens (np) 9.0 x 106 S. marcescens (p) 6.0 x 106 Citro. freundii 3.0 x D -3.0 x 106 Day 2 Day 3 Day 4 Cultivo provides uniform and consistent conditions for growth throughout the chamber. A group of bacteria that are sensitive to stressful conditions were cultured inside Cultivo in the Baker laboratory. Plates were arranged in Cultivo in four configurations: A) spaced through the chamber; B) together on one shelf in a single layer; C) close to the HEPA filter and close to the bottom of the chamber; and D) near the door. Results showed consistent growth characteristics and doubling rates no matter where plates were placed. To learn more, download our growth assessment test results.

7 Simple Operation with Customizable Control All operations are accessible through an intuitive, user-friendly touchscreen interface that gives you full control over incubator functionality LOG SETTINGS UV ⁰C SERVICE 5 4.5! 95.0 TEMP % % CO2 RH Pressure-sensitive touchscreen works with gloved hands. Real-time status updates and alarm conditions on the home screen. One-touch access to set point screens. 1. Alarm indicator 2. Low water level indicator 3. UV light indicator Customizable alarms and security settings. 4. Message waiting 5. Parameter alarm indicator Graphic data log displays for at-a-glance monitoring. USB download of data and event logs. Helps assist in providing compliance with 21 CFR Part 11 electronic record-keeping requirements. TEMP LOG SETTINGS Current Temp 34.3 C! Temp Set Point 37.0 C Temp Alarm Low 35.0 C Temp Alarm High 39.0 C SERVICE CANCEL SAVE 5

8 Improved Productivity and User Experience Cultivo has been designed around your needs, to help solve common problems and improve productivity in your laboratory. Outstanding condensation control. Cultivo is the only incubator available that provides a virtually condensation-free interior at relative humidity levels above 90%. No more time spent mopping up after dripping doors. Personnel are protected from exposure to potentially dangerous contaminants during cleanup, as well as accidental slips. Largest usable workspace leave the sweet spot behind! Cultivo offers one of the largest usable interior workspaces among incubators of the same type and size. This, combined with uniform downflow air throughout the chamber, eliminates your need to find the sweet spot of the incubator, where conditions are believed to be most favorable for cell growth. Cultures grow consistently throughout the entire chamber even next to the door. Fogless interior door. The difference is clear the solution was simple. Cultivo s heated interior door (controlled by InteliCELL ) eliminates condensation on the glass door that obstructs a clear view into the chamber. Now you can see what is happening with your cultures without opening the door every time. Ditch your water pan! Ultrasonic humidification technology (nebulizer delivery system; Ultra and Ultra Plus models) eliminates the need for a water pan, freeing your time typically spent on tedious water changes for more productive activities. More Options and Accessories Cultivo also includes additional features, options and accessories designed to enhance your research: Auto water refill option allows easy water replenishment and changes without opening the door. The exterior water reservoir is easy to monitor and fill, taking only a few minutes every few weeks. USB flash drive and alarm status relay provides for easy extraction of key data and integrates with your facility s monitoring system. alarm contacts and optional communication interfaces (analog 4-20mA signal and RS485/422 and RS232 serial ports) make Cultivo adaptable for various facility monitoring requirements. UV and H2O2 biodecontamination provide flexibility to destroy a wide variety of contaminants that infiltrate your laboratory. Spacious interior shelves hold up to 25 stacks of 90mm plates to help you maximize space.

9 Control & Kill Microbial Contaminants with UV and Hydrogen Peroxide Cultivo offers two optional protocols designed to reduce the spread of potential contaminants and to kill a wide variety of contaminating microorganisms. Customizable ultraviolet light water pan biodecontamination cycle offers more effective decontamination of microbes that may grow in the water pan (base model) or internal water reservoir (Ultra and Ultra Plus models). A customizable timer allows you to follow your institution s protocols. Vaporized hydrogen peroxide (H2O2) biodecontamination (Ultra Plus model) kills a wide variety of microorganisms within the entire chamber. Because Cultivo s H2O2 protocol is completed within 4 hours (vs. 12 hours for high temperature methods), you ll experience far less downtime than is typically required by most methods of decontamination, increasing the productivity of your laboratory. All removable interior components are autoclavable. Vaporized H2O2: Go Beyond Log Reductions, Achieve Total Biodecontamination Decontaminating methods are often evaluated based on their ability to achieve a log reduction in the number of microorganisms that can potentially contamine precious cultures. But a log reduction will not help you achieve a complete kill of all contaminants that threaten your research. In fact, some microbes may be more resistant than others, thereby posing a serious risk to re-contaminate your work. At Baker, we strive to achieve the maximum amount of protection for your research. The vaporized H2O2 biodecontamination protocol for Cultivo has been developed not to merely reduce the numbers of contaminants found wtihin your incubator; rather, it has been specifically designed to provide a complete kill of all contaminating microorganisms for total biodecontamination, beyond that which is typically expressed as a log reduction by most incubator suppliers. Compared with other methods that employ high temperatures, directed UV light or ineffective concentrations of vaporized H2O2, the Cultivo Learn more - scan the code protocol provides a more effective means of killing more forms of microbial life, including vegetative bacteria, bacterial spores, fungi, to get the vaporized H2O2 fungal spores and viruses. This keeps laboratory personnel and cultures safe from even the most resistant microbes. It also requires lower test report! temperatures, reducing the risk of burns, as well as heat conduction to adjacent incubators. It leaves no toxic residue (its harmless by-products are water and oxygen) and is more efficient, taking approximately 4 hours, rather the 12 hours typically associated with other methods. Learn more about the research and testing behind the Cultivo H2O2 biodecontamination protocol in our test report.

10 Cultivo Cultivo Ultra Evaporative (Water Pan) Humidification Cultivo Ultra Plus Ultrasonic (Nebulizer) Humidification Performance Specifications Model Number Humidity Delivery INC001 Evaporative (Water Pan) Ultrasonic (Nebulizer) External Dimensions W x 26.5 D x H (679mm W x 673mm D x 1003mm-1041mm H) Internal Dimensions 19.9 W x 21.9 D x 33.5 H (505mm W x 556mm D x 851mm H) 5.92 ft3 (0.168 m3) / mm plates Usable Interior Volume / Capacity Usable Shelf Area / Max Shelves 18.6 W x 20.1 D (472mm W x 511mm D) / 11 shelves (4 standard) Weight 244 lbs. (111 kg.) / Shipping Weight 310 lbs. (141 kg) Temperature Control Range 13 F (7.0 C) above ambient F (55.0 C) (increments of 0.2 F / 0.1 C) Temperature Accuracy +/-0.1 C Temperature Uniformity +/ C Temperature Recovery Time Within 1 C of set point (37 C) in 6 minutes CO2 Control Range 0.0% % (increments of 0.1%) CO2 Accuracy +/-0.15% CO2 Recovery Time Within 0.2% of set point (5%) in 4 minutes Relative Humidity Control Range Two operating modes: Up to 90%; 90%-95% Relative Humidity Accuracy Relative Humidity Recovery Time +/-3% Within 3% of set point (92%) in 10 minutes Contamination Prevention Within 3% of set point (92%) in 8 minutes Expansive HEPA filter providing better-than-iso Class 4 (Class 10) air cleanliness Biodecontamination Methods UV Light & Vaporized Hydrogen Peroxide () UV Light (optional) Features HEPA Filtration (better than ISO Class 4) Active Humidity Control Active Temperature Control Active CO2 Control Alarm Contacts Copper Interior Components UV Light Automatic Water Refill System Vaporized Hydrogen Peroxide Biodecontamination n/a n/a Options CO2 Supply Automatic Switchover RS232/485 or 4-20mA

11 Accessories Rolling Cart Stacking Kit Additional Shelves (4 standard, 8 additional) Etaluma Lumascope (live cell imaging) Two-Stage CO2 Gas Tank Regulator (adjustable to max of 15 psi) Portable CO2 Meter Portable ph Meter CO2 Analyzer Fyrite Kit Refill CO2 Analyzer Fluid Kit Water Pan Seismic Restraints Preventive Maintenance Kit Dry In-Line Filter HEPA Filter

12 For warranty information, please visit bakerco.com/warranty. PROUDLY MADE IN THE USA P. O. Drawer E, Sanford, ME (207) (800) Fax (207) Cultivo and InteliCELL are trademarks of The Baker Company The Baker Company. All rights reserved.