Office of Biosafety TABLE OF CONTENTS 1.0 Introduction 2.0 Responsibilities 3.0 Standard Operating Procedures 4.0 Control Measures

Transcription

1 CHEMICAL HYGIENE PLAN Office of Biosafety 2013

2 Office of Biosafety Chemical Safety Program Title Document Revision Prepared By Page Effective Date Chemical Hygiene Plan CSP Cristina Alvarez 2 of 38 February 2013 TABLE OF CONTENTS Chemical Hygiene Plan Cover Page Introduction Purpose Scope and Application Responsibilities Standard Operating Procedures Control Measures Hierarchy of Controls Employee Exposure Engineering Controls Administrative Controls and Work Practices Personal Protective Equipment and Personal Hygiene Routes of Exposure Prior Approval for the use of Hazardous Chemicals Chemical Safety Notice of Intent (NOI) Requirements and Procedures Additional Training for Employees Hazardous Chemical Handling, Storage, and Disposal General Classes of Hazardous Chemicals Special Provisions for Select Carcinogens, Reproductive Toxins, Developmental Toxins, Acutely Toxic Chemicals and Nanoparticles Hazard Identification Chemical Disposal Employee Information and Training Employee Training Employee Methods of Detection Emergency Response and Physical Hazards Injured or Exposed Employees Chemical Spill Response Fire or Explosions Laboratory Inspections Process for Non-Compliance Medical Program and Consultation Medical Examination and Written Opinion Hearing Conservation Program...24 Appendix A: Chemical Compatibility Chart...25 Appendix B: Glove Chemical Resistance Chart...31 Appendix C: Incident Report...36 References...38

3 Page 3 of INTRODUCTION This document describes the Chemical Hygiene Plan for Scott & White Healthcare laboratory personnel. The Office of Biosafety has established a written plan to ensure laboratory work areas are capable of protecting employees from health hazards associated with Hazardous Chemicals in the laboratory, and capable of keeping exposures below the Permissible Exposure Limits (PEL) specified in 29 CFR 1910 Subpart Z. The information herein satisfies the requirements for the institution to provide a written Chemical Hygiene Plan and a Hazard Communication Program in accordance with U.S. Department of Labor Occupational Safety and Health Administration (OSHA) standards 29 CFR and 29 CFR , respectively. This plan is not meant to be an exhaustive reference, but rather a guide for Scott & White Healthcare research personnel to become familiar with and conduct their operations accordingly. All users of chemicals must be familiar with the requirements set forth in this manual and applicable local, state and federal laws and regulations and must conduct their operations in accordance with them. This plan is made readily available to the employees of Scott & White Healthcare online and by contacting the Office of Biosafety. A review and evaluation of this program is conducted on an annual basis and updated as necessary. The persons responsible for implementation of this plan are Scott & White Healthcare Office of Biosafety personnel. Questions regarding this plan should be directed to the Office of Biosafety. Office of Biosafety: OBS@sw.org Biosafety Officer: Francis J. Novembre, Ph. D fnovembre@sw.org Safety Office Coordinator: Cristina A. Alvarez, MPH caalvarez@sw.org

4 Page 4 of Purpose The purpose of this Chemical Hygiene Plan (CHP) is to provide a general guide for handling hazardous chemicals in the laboratory by establishing basic safety principles for procedures, equipment, and work practices that should be employed to protect students, laboratory workers, and other personnel from health hazards associated with hazardous chemicals. 1.2 Scope and Application The Chemical Hygiene Plan is part of Scott & White s compliance to regulations set forth by the US Department of Labor Occupational Safety and Health Administration (OSHA) standard entitled Occupational Exposure to Hazardous Chemicals in Laboratories (Code of Federal Regulations, 29 CFR ), Hazard Communication (Code of Federal Regulations, 29 CFR ) and the Texas Hazard Communication Act (Chapter 502 of the Texas Health and Safety Code). Hazardous chemicals are defined by OSHA as a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees. Additionally, OSHA defines a laboratory as a workplace where relatively small quantities of hazardous chemicals are used on a non-production basis. Furthermore, while laboratory workers are defined by OSHA under the definition of employee as an individual employed in a laboratory workplace that may be exposed to hazardous chemicals in the course of his or her assignments, students are also covered under this CHP. Where the scope of hazards is not adequately addressed by this general document, specific Standard Operating Procedures must be developed by departments and laboratory supervisors. This CHP applies to the following personnel: Scott & White employees conducting research in laboratories Students conducting research in Scott & White research laboratories on the Scott & White Temple Campus Any researchers conducting research in Scott & White animal facilities This CHP does not apply to: The preparation of medical substances and doses for the pharmacy or other administration of medicines to patients Hazardous chemicals use that does not meet the definition of laboratory use Hazardous chemicals that do not offer the potential for exposure

5 Page 5 of RESPONSIBILITIES Safety is the responsibility of all personnel within the Scott & White Healthcare System. The responsibilities of individuals are listed below and are expected to be understood and conducted in accordance with this plan. 2.1 Office of Biosafety: Establish, maintain, and revise the Chemical Hygiene Plan (CHP) annually. Create and revise safety rules and regulations. Serve on appropriate safety committees. Monitor procurement, use, storage, and disposal of chemicals. Conduct regular inspections of the laboratories, preparations rooms, and chemical storage rooms, and submit detailed laboratory inspection reports to administration. Maintain inspection, personnel training, and inventory records. Oversee chemical inventory updates. Assist laboratory supervisors in developing and maintaining adequate facilities. Know current legal requirements concerning regulated substances. Seek ways to improve the chemical hygiene program. Encourage laboratory personnel to attend specialized training that is provided by the institution (i.e., first-aid training, CPR training, fire extinguisher training, and infectious substances shipping training). Notify employees of the availability of medical attention under the following circumstances: 1. Whenever an employee develops signs or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory; 2. Where exposure monitoring reveals an exposure level routinely above the action level for an Occupational Safety and Health Administration regulated substance for which there are exposure monitoring and medical surveillance requirements; 3. Whenever a spill, leak, explosion, or other occurrence resulting in the likelihood of a hazardous exposure occurs, a medical consultation to ascertain if a medical examination is warranted. 2.2 Division/Department Director: Assumes responsibility for personnel engaged in the laboratory use of hazardous chemicals. Provides the Office of Biosafety with the support necessary to implement and maintain the CHP. Provides budgetary arrangements to ensure the health and safety of the departmental personnel, visitors, and students.

6 Page 6 of Principal Investigator: Ensures that laboratory personnel comply with the CHP and do not operate equipment or handle hazardous chemicals without proper training and authorization. Always wears personal protective equipment (PPE) that is compatible to the degree of hazard of the chemical. Follows all pertinent safety rules when working in the laboratory to set an example. Reviews laboratory procedures for potential safety problems before assigning to other laboratory personnel. Ensures that visitors follow the laboratory rules and assumes responsibility for laboratory visitors. Ensures that PPE is available and properly used by each laboratory employee and visitor. Maintains and implements safe laboratory practices. Monitors the facilities and the chemical fume hoods to ensure that they are maintained and function properly. Contacts the appropriate person, as designated by the department chairperson, to report problems with the facilities or the chemical fume hoods. Ensures laboratory signs are up to date with current hazards and emergency contact information. Ensures the laboratory has an up to date chemical inventory. 2.4 Lab personnel: Reads, understands, and follows all safety rules and regulations that apply to the work area. Plans and conducts each operation, laboratory class, or research project in accordance with the departmental and institutional CHP. Promotes good housekeeping practices in the laboratory or work area. Communicates appropriate portions of the CHP to students in the work area. Notifies the supervisor of any hazardous conditions or unsafe work practices in the work area. Uses PPE as appropriate for each procedure that involves hazardous chemicals. Immediately reports any job related illness or injury to the supervisor.

7 Page 7 of STANDARD OPERATING PROCEDURES For laboratory work at Scott & White Healthcare which involves the use of Hazardous Chemicals, standard operating procedures (SOP) have been developed in order to reduce potential Safety and/or Health Hazards caused by such use. These procedures include various engineering and administrative controls as well as work practices and procedures and personal protective equipment use. Individual administrative units, laboratories or research groups are required to develop more detailed procedures as their situations warrant. In all situations, individual faculty or staff will be responsible for enforcing adequate safety and hygiene measures in laboratories they supervise. The specific procedures implemented by Scott & White Healthcare Office of Biosafety for all laboratories are as follows. Scott & White is a tobacco-free campus. Smoking is not permitted in any of the Scott & White Healthcare premises including laboratories. Eating, drinking, food storage and the application of cosmetics or contact lenses are not permitted in areas where hazardous chemicals are used, or stored and must be done only in well-defined designated non-chemical areas. Do not perform unauthorized experiments. Laboratory experiments should not be left unattended. Horseplay, practical jokes or other behavior that might confuse, startle, or distract another worker while working with hazardous materials are not permitted. Exits, aisles and safety equipment must be kept clear of any obstructions, such as equipment, furniture, trash, etc. Work areas and floors should be kept clear of excessive storage. Hazardous liquid chemicals should not be stored above eye level. Do not taste or smell chemicals. Use proper equipment that is in good condition. For example, never use chipped or cracked glassware. Shield pressurized or vacuum apparatus and safeguard against bumping or overheating. A chemical inventory should be maintained, updated regularly and reviewed at least annually. Any thermometers and reagents that contain mercury should be eliminated or replaced. When inserting glass tubing into stoppers, lubricate the tubing and protect hands from being cut in the event the tubing slips and breaks. Use of other hazardous materials such as heavy metals and halogenated solvents should be eliminated or reduced. Plan appropriate protective procedures and the positioning of all equipment before beginning any operation. Follow the appropriate standard operating procedures at all times in the laboratory.

8 Page 8 of 38 Always read the Safety Data Sheets (SDS) and the label before using a chemical in the laboratory. Wear long pants and closed toe shoes in the laboratory. Everyone must wear appropriate PPE, including a laboratory apron or coat, at all times in the laboratory. Wear appropriate gloves when handling hazardous materials. Inspect all gloves for holes and defects before using. Mouth pipetting is not allowed. Mechanical devices must be used for suction. Use appropriate ventilation such as laboratory chemical hoods when working with hazardous chemicals. Know the location and proper use of the safety equipment (i.e., eyewash unit, safety shower, fire extinguisher, first-aid kit, fire blanket, emergency telephone, and fire alarm pulls). Be aware of the hazards posed by the work of others in the laboratory and any additional hazards that may result from contact between materials and chemicals from different work areas. Keep laboratory door closed at all times. Make others in the laboratory aware of any special hazards associated with your work. Notify supervisors of any chemical sensitivities or allergies. Report all injuries, accidents, incidents, and near misses immediately to supervisors and the Office of Biosafety. For liability, safety, and security reasons, do not allow unauthorized persons in the laboratory Report any unsafe conditions to the laboratory supervisor or Office of Biosafety. Properly collect and label all chemical wastes and contact the Office of Biosafety for disposal. 4.0 CONTROL MEASURES 4.1 Hierarchy of Controls A hierarchy of controls has been traditionally used as a way to implement feasible and effective controls to reduce employee exposure. The controls are adjusted so the ones at the top of the list are potentially more effective and protective than those at the bottom. The list below is the hierarchy to be used in Scott & White Healthcare Research facilities. Elimination and Substitution of hazards is most effective, but also tends to be the most difficult to implement in an existing process. These work best at the design and development stages. Examples: elimination of hazards altogether or substitution of one hazardous chemical with one that is less or non-hazardous

9 Page 9 of 38 Engineering controls are items used to remove a hazard or place a barrier between the worker and the hazard. Examples: fume hood, biosafety cabinet, emergency showers and eyewash stations Administrative controls are policies, rules or programs that are developed by the institution to protect and minimize employee exposure to workplace hazards. Examples: protocol approvals, training, appropriate chemical storage, signs and labeling Work practices are procedures for safe and proper work that are used to reduce the duration, frequency or intensity of exposure to a hazard. Examples: standard operating procedures, standard microbiological practices Personal Protective Equipment (PPE) is protective gear needed to keep workers safe while performing their jobs when no other controls are feasible. Examples: lab coat, gloves, safety goggles, respiratory protection 4.2 Employee Exposure An initial determination of an employee s exposure to any substance regulated by an OSHA Standard which requires monitoring is made if there is reason to believe exposure levels for substances routinely exceed the Action Level or in the absence of an Action Level, the Permissible Exposure Limit (PEL). If initial monitoring demonstrates employee exposure over the Action Level and/or Permissible Exposure Limit, exposure monitoring and cessation of monitoring shall be in accordance with the relevant OSHA Standard. Where the use of respirators is determined to be necessary to maintain exposure below permissible limits, appropriate respiratory protection shall be provided at no cost to employees. Respirators shall be selected and used in accordance with requirements in OSHA Standard titled Respiratory Protection 29 CFR (b) (d) (e) & (f). 4.3 Engineering Controls Engineering controls must be tested and certified on a scheduled basis to ensure proper functionality. The various engineering controls are listed below with the minimum testing requirements. All testing or certification of equipment must be recorded in a visible area near or on the control.

10 Page 10 of 38 Engineering Control Chemical Fume Hood Biosafety Cabinet Eyewash station Emergency Shower Hand washing facilities Minimum testing required Annual Annual Weekly if in laboratory Weekly if in laboratory none As general laboratory ventilation cannot be relied upon to protect personnel from localized exposures to hazardous levels of airborne chemicals, engineering controls such as laboratory fume hoods and other local exhaust systems are often necessary to provide additional exposure control. In general, laboratory fume hoods are recommended whenever using hazardous chemicals that: Having high acute toxicity, or which are carcinogens, or reproductive toxins. Have a permissible exposure limit of less than 50 ppm (or 0.25 mg/m3 for particulate matter). Are volatile (e.g. solvents) or are easily dispersible in air (e.g. dust). Are odiferous A biological safety cabinet is not an equivalent substitute for a chemical fume hood and must never be used for handling hazardous chemicals. Biological safety cabinets should be used when handling infectious materials. 4.4 Administrative Controls and Work Practices The Office of Biosafety should be notified of any highly hazardous chemicals or materials being used in the laboratory. The Chemical Hygiene Officer (CHO) will determine if a Notice of Intent (NOI) is required prior to use of any hazardous chemicals or agents in the laboratory. Please refer to section 5.0 for more information regarding the prior approval process. In some instances, submission of a protocol or NOI to other Scott & White Healthcare Committees, such as IACUC, may require Office of Biosafety review prior to approval. The Office of Biosafety is available for assistance with completing or submitting a NOI or other hazard forms required by Scott & White safety committees. The use of hazardous chemicals may require more specific work practices or procedures in addition to the Standard Operating Procedures provided in section 3.0 of this document. The Office of Biosafety highly recommends the use of laboratory developed Standard Operating Procedures for any hazardous chemicals present and used. When necessary, the Office of Biosafety will develop SOPs for specific chemicals that will be accessible on the Biosafety webpage under Chemical Safety at

11 Page 11 of Personal Protective Equipment and Personal Hygiene Personal protective equipment and personal hygiene are two basic aspects of laboratory safety. Wearing appropriate personal protective equipment and practicing good personal hygiene, as described below, will minimize exposures to hazardous chemicals during routine use and in the event of an accident. Defective or damaged personal protective equipment should never be used. Attire: Personnel must wear a lab coat in the laboratory, ensure legs and feet are covered by wearing long pants and closed toe shoes (no sandals, open-toed shoes, or shorts), and confine any loose clothing and long hair. An apron can be used in addition to the lab coat but not as a substitute. Gloves: Gloves are essential when working with hazardous substances. The proper gloves will prevent skin absorption, infection or burns. All glove materials are not equally effective in protection from chemical hazards. In many cases, latex examination gloves do not provide adequate protection from hazardous chemicals. Consult a chemical resistance chart such as the one found in Appendix B or from your glove manufacturer or contact the Office of Biosafety for assistance in appropriate selection. Many glove manufacturers provide chemical resistance charts for their products online. Eye protection: All personnel including students, staff and visitors in laboratories must wear safety glasses or goggles at all times where potential eye hazards exist. Goggles are required when chemical splashes are possible. If contact lenses are to be worn, the eyes should be protected by goggles when in the lab. Prescription glasses are not adequate eye protection alone. They may be used in addition to one of the above listed protective measures. Face shields: Full-face shields must be worn when conducting a procedure that may result in a violent reaction. Full-face shields provide protection to the face. It is recommended that the shields are used with safety goggles when working with hazardous chemicals since the face shields do not entirely protect the eyes from chemical exposure. Personal hygiene: Hands should be washed frequently throughout the day. More specifically, they should always be washed before leaving the lab, after contact with any hazardous material, after removing gloves, and before eating. If at any time personnel are unsure of appropriate PPE, the Office of Biosafety should be contacted for assistance in proper PPE selection.

12 Page 12 of Routes of Exposure There are several routes of exposure to chemicals in the laboratory. The following briefly describe the routes and the recommended controls for each. Inhalation is the most common route of entry of chemicals into the body. Engineering controls are the best option to eliminate or minimize the hazards from significant inhalation exposure. The use of properly functioning fume hoods or some other local exhaust system is often required to minimize exposure. Substitution of chemicals with less volatile or toxic chemicals, or substituting liquid or solid chemicals for gaseous chemicals is also a means of control. Stricter controls may be required for the use of extremely toxic chemicals. Some administrative controls include: Reducing the frequency of use and length of time with the chemical Restricted access to an area where hazardous chemicals are used Posting proper signage to indicate the special hazards Allow procedures with certain nuisance odors to be performed after typical office hours, when most staff has left. If engineering and administrative controls are not an option, the use of personal protective equipment may be required to reduce inhalation exposures. If respirators are worn by laboratory employees, requirements of the OSHA Respiratory Protection Standard (29 CFR ) must be met. This standard requires training on the proper use of respirators, medical surveillance to ensure the user is capable of wearing a respirator, and fit testing to ensure the respirator fits properly. A lab worker or his/her supervisor should contact the Office of Biosafety in the event that respiratory protection is needed to control exposures to hazardous chemicals. Skin / Eye Contact: To reduce the risk of a chemical entering the body via skin or eye contact, engineering controls including substitution and appropriate ventilation, should be used as described in Inhalation Hazards. The use of personal protective equipment, such as eye protection, face shields, gloves, appropriate shoes, lab coats, and other protective equipment, is another effective means of preventing skin and eye contact. Administrative controls include enforcing policies regarding skin and eye protection, and discarding or repairing any broken glassware or equipment.

13 Page 13 of 38 Injection/Cuts Exposure: Injection or cuts most commonly occur while handling needles or sharps. Work practices to help prevent these include reducing the use of sharps as much is reasonably achievable, dispose of any broken glassware in the glass box or rigid leak-proof sharps container, do not recap, bend, break or manipulate any needles or syringes. The use of needlesafe devices is recommended when available. Sharps containers or broken glass boxes should never be filled more than ¾ full. Personal protective equipment such as cut or puncture resistant gloves, laboratory coats, appropriate shoes, and other protective equipment should also be worn as a means of preventing exposure. Ingestion: Ingestion of chemicals is the least common route of entry into the body. Laboratory workers can easily ingest chemicals into the body via contaminated hands if they are not washed prior to eating, smoking or sticking part of the hand, or a writing tool that has been in contaminated hands, into the mouth. Use engineering controls to isolate the hazardous substance so that minimal contact is required. Administrative controls and work practices include no mouth pipetting and designating a non-chemical or non-laboratory area where eating, drinking, and cosmetic application is permitted. Good personal hygiene such as frequent hand washing is also a means of preventing ingestion. Personal protective equipment such as gloves should be properly selected per the recommendations listed in the SDS and used. 5.0 PRIOR APPROVAL FOR THE USE OF HAZARDOUS CHEMICALS Hazardous chemicals that meet the requirements below must be registered with the Office of Biosafety prior to use in the laboratory. Contact the Office of Biosafety for instructions on registering your chemical for approval. The Office of Biosafety and Biosafety Officer retain the rights to inspect compliance with these procedures and suspend a protocol, if necessary. For any questions or assistance with this process please contact the Office of Biosafety. 5.1 Chemical Safety Notice of Intent (NOI) Requirements A chemical is administered (via IP, IV, food, water, etc.) to a research animal for experimental purposes. (Chemical Hazard Form will be completed through IACUC) AND/OR The chemical has - or is expected to have - one or more of the following characteristics listed in the table below:

14 Page 14 of 38 Hazard Criteria Examples Acetyl Cholinesterase Inhibitors All Parathion, Physostigmine, Diisopropylfluorophosphate Carcinogens Regulated by OSHA carcinogen Listed by NTP as a Part A or Part B carcinogen Listed by IARC as a Group 1, 2A, or 2B carcinogen Benzidine, Ethyleneimine Cyclophospamide, Tamoxifen, Doxorubicin HCl, ENU, MNU, PCBs, Streptozotocin Nickel compounds, Dimethyl sulfate, Etoposide (VP-16), Urethane Other known carcinogens Azoxymethane Chemotherapeutics All Busulfin, Cisplatin, Paclitaxel, Erlotinib, Fludara, Sutent, Pentostatin Highly regulated chemicals RCRA P-listed chemicals Heptachlor, Physostigmine RCRA U-listed chemicals DDT, ENU, MNU, Streptozotocin RCRA D-listed chemicals Cadmium, Chromium, Lead, Mercury EPA PIC Banned or severely restricted pesticides DDT, Aldrin, Dieldrin, Heptachlor Severe environmental PCBs hazards Hazard Criteria Examples Irritants (cause a severe Strong Irritants 5-FU, Phosgene reversible inflammatory effect at the site of contact) Irreversible Illness All MPTP, Thalidomide Mutagens Known or suspected to cause mutations in humans BRDU Sensitizers (can cause an allergic reaction after repeated exposure) Teratogens Known to cause mutations in animals All Known or suspected to cause teratogenic effects in humans Bleomycin Sulfate Paclitaxel, Malathion, Isocyanates, Nickel Salts 486, Thalidomide Known to cause teratogenic Bleomycin Sulfate, Erlotinib, Fludara effects in animals Toxics and Toxins LD50 (oral, rat) <500mg/kg Tetrodotoxin, picrotoxin, 5-FU, Rotenone, Chloropyrifos, DMBA LD50 (dermal, rat) <1,000 mg/kg LC50 (rat) <200 pm Acute or chronic organ toxicity Aldrin, Chloropyrifos, Dieldrin Acrolein, Diisopropylfluorophosphates, Dimethyl Sulfate, Parathion, Phosgene Potassium Cyanide, PCBs, Nickel Sulfide

15 Page 15 of Chemical Specific Training for Employees Depending on the chemical to be used the Laboratory Safety Training may be sufficient, but in other cases the Office of Biosafety may require additional training due to the hazards associated with the chemical and how it will be handled. The PI will be required to provide additional Hazard Specific Training that is to include the hazards of the chemical, Standard Operating Procedures to be used, proper disposal procedures, as well as emergency response procedures to all laboratory employees. The PI shall record the training date, the personnel in attendance with a sign in sheet, and training information provided to personnel. These records should be stored in the Laboratory Safety Binder with other training records and NOI information. 6.0 HAZARDOUS CHEMICAL HANDLING, STORAGE, AND DISPOSAL 6.1 General Classes of Hazardous Chemicals There is wide variation in the hazards associated with chemicals. Understanding these hazards along with minimizing the amount used and stored within the lab decreases the chance of injury. Chemicals should be separated at a minimum by the following types. Corrosives: Corrosives are acutely toxic chemicals that cause harmful effects after single exposure and destruction of living tissue at site of contact. Examples: Strong Acids- hydrochloric acid, nitric acid Strong Bases- Sodium Hydroxide, Ammonia Organic Solvents- Phenol Chemical Storage: Store concentrated acids and bases separately Keep corrosives away from organic chemicals and combustible materials Must use secondary catch basins for concentrated acids and bases Must be stored in a corrosives cabinet Work Practices: Always protect skin, eyes and face Wear lab coat/ protective apron Always wear long pants and closed toe shoes Use corrosion resistant gloves to protect hands Wear safety goggles to protect eyes and a face shield if required for additional protection

16 Page 16 of 38 Flammable liquids: These are defined by flash point, which is the minimum temperature at which the liquid will vaporize (evaporate). Chemicals with a flash point less than 141 o F (60 o C) are considered flammable. Examples: Ethyl Alcohol Isopropyl Alcohol Methanol Chemical Storage: No more than 10 gallons of flammable liquids should be stored outside of an approved flammable storage cabinet No more than 60 gallons should be stored per flammable storage cabinet and no more than 3 storage cabinets per lab. Work Practices: Always handle flammables in areas free of ignition sources Never heat flammables with an open flame Dilute flammable vapors with using ventilation device (chemical fume hood) Use refrigerators certified for storage of flammable materials Store flammables near fume hood to encourage use of fume hood when transferring chemicals Know the location of your fire extinguishers, fire pull alarms and nearest exit Oxidizers: These can ignite or accelerate fires. Examples: Peroxides Nitrates Perchlorates Hypochlorates Chemical Storage: Store away from flammables and combustible materials Store away from reducing agents Maintain the minimum quantity needed and dispose of unneeded material immediately Work Practices Minimize the quantities on hand Exercise caution when mixing with flammable or combustible materials and use small amounts to reduce generation of heat and control the reaction.

17 Page 17 of 38 Reactives: Reactive chemicals are unstable compounds which react violently or explosively when exposed to other chemicals or certain physical conditions. They can produce toxic fumes, fires, and explosions Examples: Water reactive chemicals Pyrophors react with air Peroxide forming chemicals can cause shock sensitive explosives Picric acid and Trinitrotoluene (TNT) Chemical Storage Consult MSDS for storage information Work Practices Bring only quantities needed for immediate use in the laboratory Date all reactive chemicals as soon as they are received Do not open material after the expiration date Compressed Gases and Cryogenic Liquids: These can create pressure hazards and can also create health hazards and/or flammable atmospheres. These undergo substantial volume expansion when released to air, potentially depleting workplace oxygen content to hazardous levels. Examples: Oxygen Carbon Dioxide Liquid Nitrogen Chemical Storage Store compressed gas cylinders away from heat sources, flammable and highly combustible materials Gas cylinders should be individually secured (e.g. strapped or chained down) to a wall, sturdy surface or in a base stand Work Practices: Check connections and hoses regularly for leaks Replace valve caps when cylinders are not in use or before moving Segregate and clearly mark Full and Empty cylinders Wear at a minimum lab coat, safety glasses, and closed toe shoes. Wear any additional PPE as necessary. Nanomaterials: Nanoparticles can be natural or engineered and are a collection of tens to thousands of atoms approximately 1 to 100 nanometers in diameter. Engineered nanoparticles are produced and designed with very specific properties related to shape, size, surface properties and chemistry. The health effects of exposure to nanomaterials are still not fully understood.

18 Page 18 of 38 Examples: Carbon fullerenes Carbon nanotubes Metal oxide nanoparticles Quantum dots Work Practices: A combination of controls (engineering controls, work practices, and personal protective equipment) is required to minimize potential exposures to themselves and others. Work with nanomaterials must be approved through the Scott & White Healthcare IBC before use. 6.2 Special Provisions for Select Carcinogens, Reproductive Toxins, Developmental Toxins, Acutely Toxic Chemicals, and Nanoparticles Special provisions for protection for employees who work with particularly hazardous substances will be established according to OSHA regulations. The provisions for these particularly hazardous substances are as follows: Establish work practices according to hazard Establish designated work areas Use containment devices (chemical fume hoods or glove boxes) Use proper PPE Establish procedures for chemical disposal Establish decontamination procedures These particularly hazardous substances include those with a high degree of acute toxicity, or those which are highly toxic and may be fatal or cause damage to target organs as a result of a single exposure or exposures of short duration. Some particularly hazardous substances are described below. Select Carcinogens: Chemicals capable of causing cancer. These can cause damage after repeated or long-duration exposure. Adverse effects are not immediate. Example: Benzene, Formaldehyde Reproductive toxins: Affect various aspects of reproduction including but not limited to gestation, lactation, fertility. Example: Benzene, Xylene Developmental Toxins: Affect fetus during pregnancy and can cause malformation or retarded growth. 6.3 Hazard Identification and Labeling Hazard identification and labeling assist to ensure personnel are aware of the hazards and risks of the chemicals in the workplace. Scott & White Healthcare assures labels on incoming containers of hazardous chemicals are not removed or defaced prior to delivery to the laboratory. Material Safety Data Sheets and Safety Data Sheets that are received with incoming shipments of hazardous chemicals shall be stored within the laboratory and be readily accessible to laboratory employees.

19 Page 19 of 38 All chemicals must have a label in English listing the full name of the chemical and its hazards. It should also have the date the chemical was received and the date it was opened. If an expiration date is provided, but not easily identified on the label or container, it should be visibly written on the label or container. If the chemical is transferred to a new container, it must be labeled in English with the full chemical name, hazards associated, and any pertinent date information. Laboratories must have appropriate signage posted at the entrance listing the hazards found within the laboratory, at least two emergency contacts and any entry requirements, such as personal protective equipment. The Office of Biosafety can assist in providing the appropriate signage for your laboratory. 6.4 Chemical Disposal Chemicals shall not be disposed of through sanitary sewer or non-hazardous waste streams such as office or laboratory trash cans. Chemical waste should be disposed per the Material Safety Data Sheet or Safety Data Sheet. The Office of Biosafety should be contacted by at OBS@sw.org for chemical waste disposal. The following information will need to be provided when scheduling a pickup: Chemical name or list of chemicals Amount of each chemical to be picked up Contact person and information Laboratory location Chemical waste should be secured in appropriate containers with a secondary catch basin in a low traffic area to prevent accidental release. All chemical waste containers should be properly labeled in English with the name of the chemical and the hazards. Contact the Office of Biosafety for proper disposal procedures for any mixed waste, such as biological and chemical waste. 7.0 EMPLOYEE TRAINING AND INFORMATION Scott & White Healthcare provides laboratory employees with information and training on hazardous chemicals at time of initial assignment to ensure employees are aware of the hazards present in their work area. If there is a work specific hazard, direct supervisors are responsible for providing employees with more specific training.

20 Page 20 of Employee Training The Office of Biosafety provides several safety trainings. The training schedule along with registration links for all the trainings offered can be found on the Office of Biosafety website at The following description provides an overview of the training needed for all research laboratory personnel: Laboratory Safety: This training is required annually for all individuals who will be involved in any form of laboratory work or experiments. The training covers general laboratory safety, a brief overview of some chemical, biological, radiological and physical hazards. It also reviews the OSHA Hazardous Chemicals in the Laboratory Standard (29 CFR ), the Chemical Hygiene Plan, and controls. Any laboratory agent or technique specific training required is the responsibility of the PI or other laboratory employee properly trained to do so. No employee shall be allowed to perform work unsupervised until properly trained. 7.2 Employee Methods of Detection The following diagram represents several methods of detection employees may use to detect the presence or release of a hazardous chemical in the work area. Visual Detection Smell Monitoring Devices/ Direct Reading Instruments Employee Monitoring Chemical Spill Presence of an odor Oxygen monitors When required by OSHA (e.g. Formaldehyde) Leaking Chemical Container When an employee develops signs and symptoms of chemical exposure (e.g. carbon monoxide) 7.3 Physical Hazards and Emergency Response Working with chemicals poses various physical risks such as slips, falls, fires, or explosions. It is important to identify these potential risks and prevent them by maintaining clean work areas, reviewing Safety Data Sheets for storage and handling procedures, reviewing the literature on the chemicals, discussing projects with principal investigators prior to beginning work, and using appropriate protective devices or equipment. In the event of a chemical spill or release, employees will notify any personnel in the area of the spill by verbal notification or warning signage and follow the provided procedures as follows.

21 Page 21 of Injured or Exposed Employees If an employee has been injured or exposed, immediate care is the priority. If the area does not pose an immediate danger (e.g. fire, explosion, hazardous air quality, further injury to others), then use the closest emergency equipment available. If the area does pose an immediate danger, exit the area and use emergency equipment in a safe area. The following graphic shows the flow of procedures for an injured person. The S&W 24- hour help line for emergencies can also be contacted for emergency assistance. Call (254) to access the pager system, and then enter 0450 as the pager number and your phone number to display. Immediate Care: Use the emergency eye wash station, emergency shower, handwashing facilities, or follow lab specific incident protocol Notify Supervisor: Immediately notify your supervisor and complete an Employee Accident Form ( Medical Attention: Visit S&W Employee Health Services(Bldg 19) If after hours, contact 24-hr help line above and visit S&W Emergency Department for treatment. Incident Reporting: Complete an Adverse Event form and also notify the Office of Biosafety within 24 hours. ( Follow Up: Follow up with your health care professional treatment plan as recommended.

22 Page 22 of Chemical Spill Response If employees have a simple spill posing no immediate danger and any injured employees have been provided care, the following procedures should be followed along with any SDS spill response procedures. Notify any personnel in the area by verbal notification or written signage and evacuate the laboratory for at least 30 minutes to allow any aerosols to settle. Wear appropriate PPE: Put on appropriate PPE prior to cleaning the spill. Prevent or control the spreading: Use absorbent materials to contain the spill and prevent further contamination. Collect any residues: Collect any residues and dispose of as hazardous materials. If any broken glass or sharps are involved use forceps, a broom and dustpan, or other method of collecting residues. Decontaminate the area and equipment: All areas and equipment should be properly decontaminated following the spill to prevent further exposure of employees or contamination of other areas. Notification and Incident Reporting: Supervisors should be notified of the spill and an incident report should be completed and returned to the Office of Biosafety. Notification and Incident Reporting: Supervisors should be notified of the spill and an incident report should be completed and returned to the Office of Biosafety. **If employees do not feel comfortable cleaning the spill, the Office of Biosafety should be contacted immediately for assistance.

23 Page 23 of Fires or Explosions In the event of a fire or explosion in the lab, all personnel should immediately notify others in the area to evacuate and activate the nearest fire alarm pull station. Personnel should always use the stairs to exit the building. The elevators should never be used during a fire. Personnel should meet at the designated meeting space for the building in order to account for personnel. If there is a small fire (< office trash can) and the employee is trained and comfortable using an extinguisher, the employee can use the acronym PASS (Pull the pin, Aim the nozzle, Squeeze the trigger, and Sweep side to side) to extinguish the fire. The employee should always keep the exit at their back in order to avoid being surrounded by the fire and maintain egress. 8.0 LABORATORY INSPECTIONS The Office of Biosafety performs laboratory inspections prior to approval of a NOI for the use of biohazardous or special chemical hazards (see section 5.0 above). This is done to ensure the laboratory meets the appropriate safety requirements. The inspection includes compliance with regulations required by federal, state and local laws, as well as standards and recommendations from organizations such as ANSI, CDC and NIH. A copy of the inspection can be found at PROCESS FOR NON-COMPLIANCE Deficiencies identified during laboratory inspections or non-compliance with approved NOI procedures will be addressed as follows: Step One - Verbal Notification: If, during a routine evaluation or inspection, a problem involving procedures is observed, a verbal recommendation will be provided. If upon receipt of a verbal recommendation, the laboratory staff or the Environmental Health and Safety staff takes immediate steps to correct the problem, then no further response regarding the discrepancy will be requested. Step Two - Written Notification: Following the survey, a written summary of the findings and recommendations including corrections during the survey will be sent to the PI responsible for the laboratory. The PI will then be requested to take corrective action within 30 days. Verbal, , or written response is requested. Step Three - Documentation: A list of discrepancies will be maintained by the Office of Biosafety and a follow-up will be conducted after 30 days of the initial deficiency identification to determine if corrective actions have been taken. Step Four - Follow-up: If the follow-up reveals that the same deficiency exists, notification of this situation may be sent to both the PI and the Department Chair. A written response from the PI shall be sent to the Office of Biosafety detailing specific steps taken to ensure correction of the discrepancy.

24 Page 24 of 38 Step Five - Final Action: If the problem persists, the issue will be handled on a case by case basis. The entire case history will be presented to administrative reviewers for disciplinary action. Any operation causing a high or unacceptable risk to employees or personnel exposure to any chemical hazard will be suspended immediately by the Office of Biosafety/IBC without regard to the above procedure. In the event of this action, the situation will be promptly reviewed by the administrative reviewers MEDICAL PROGRAM AND CONSULTATION Scott & White Healthcare provides all employees who work with hazardous chemicals an opportunity to receive medical attention, including any follow-up examinations which the examining physician determines to be necessary, for the following circumstances: Whenever an employee develops signs or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory, the employee is provided an opportunity to receive an appropriate medical examination. Where exposure monitoring reveals an exposure routinely above the Action Level, or in the absence of an Action Level above the Permissible Exposure Limit (PEL), for an OSHA regulated substance for which there are exposure monitoring and medical surveillance requirements. Medical Surveillance shall be established for the effected employee as prescribed by the particular OSHA Standard. Whenever an event takes place in the work areas such as a spill, leak, explosion or occurrence resulting in the likelihood of a hazardous chemical exposure, the effected employee(s) is provided an opportunity for a medical consultation. This consultation is for the purpose of determining the need for a medical examination. An appropriate medical examination is provided as necessary Medical Examinations and Written Opinion Medical examinations and consultations are performed or arranged through S&W Employee Health (Bldg 19), are provided at NO COST to employees without loss of pay and at a reasonable time and place. The physician shall be provided with the following: The identity of hazardous chemical(s) the employee may have been exposed to; A description of the conditions under which the exposure occurred including quantitative exposure data, if available; and A description of the signs and symptoms of exposure the employee is experiencing if any.

25 Page 25 of 38 A written opinion from the examining physician for an examination or consultation which is required by the Standard is obtained. The written opinion shall not include any findings/diagnoses which are not related to an occupational exposure. This opinion shall include: Any recommendation for further medical follow-up; The results of the medical examination and any associated tests; Any medical condition that may be revealed in the course of the examination which may place the employee at increase risk as a result of exposure to a hazardous chemical found in the workplace; and A statement the employee has been informed by the physician of the results of the consultation or medical examination and any medical condition that may require further examination or treatment The S&W Hearing Conservation program is also available to S&W employees. The program informs workers of noise hazards and a working environment that provides effective means of protection against hearing loss. The S&W Healthcare Hearing Conservation Program establishes appropriate and effective engineering and administrative controls, instructs on and provides personal hearing protection, and works to ensure proper use of hearing protection equipment by staff. Environment of Care/Safety Compliance maintain the Hearing Conservation Program and should be contacted regarding enrollment in this program. The office can be reached at (254) For more information about the Hearing Conservation Program please visit ion.pdf.

26 Page 26 of 38 APPENDIX A: Chemical Compatibility Chart The following chart provides compatibility information for various chemical groups. Please refer to the group descriptions for more specific examples of each group. This information can also be found online on the IBC website,

27 Page 27 of 38 Group1: Inorganic Acids Chlorosulfonic acid Hydrochloric acid (aqueous) Hydrofluoric acid (aqueous) Hydrogen chloride (anhydrous) Hydrogen chloride (anhydrous) Nitric acid Oleum Phosphoric acid Sulfonic acid Group 2: Organic Acids Acetic acid Butyric acid (n-) Formic acid Propionic acid Rosin Oil Tall Oil Group 3: Caustics Caustic potash solution Caustic soda solution Group 4: Amines and Alkanoamines Aminoethylethanolamine Aniline Diethylenetriamine Diisopropanolamine Dimetheylamine Ethylenediamine Hexamethylenediamine 2-Methyl-5-ehtylpyridine Monoethanolamine Morpholine Pyridine Triethanolamine Triethylamine Triethylenetetramine Trimethlyamine Group 5: Halogenated Compounds Allyl chloride Carbon tetrachloride Chlorobenzene Chloroform Chlorohydrines, crude Dichlorobenzene (o-) Dichlorobenzene (p-) Dichlorodifluoromethane Dichloroethyl ether Dichloropropane Dichloropropene Ethyl chloride Ethylene dibromide Ethylene dichloride Methyl bromide Methyl chloride Methylene chloride Monochlorodifluoromethane Perchloroethylene Propylene dichloride 1,2,4-Trichlorobenzene 1,1,1-Trichloroethane Trichloroethylene Trichlorofluoromethane Group 6: Alcohols, Glycols, Glycol Ethers Allyl alcohol Amyl alcohol 1,4-Butanediol Butyl alcohol (iso, n, sec, tert) Butylene glycol Corn syrup Cyclohexyl alcohol Decyl alcohol (n, iso) Dextrose solution Diacetone alcohol Diethylene glycol Diethylene glycol dimethyl ether Diethylene glycol monobutyl ether Diethylene glycol monomethyl ether Diisobutyl carbitol Dipropylene glycol Dodecanol Ethoxylated dodecanol Ethoxylated pentadecanol Ethoxylated tetradecanol Ethoxylated tridecanol Ethoxytriglycol Ethyl alcohol Ethyl butanol 2-Ethylbutyl alcohol 2-Ethylhexyl alcohol Etheylene glycol Etheylene glycol monobutyl ether Etheylene glycol monoethyl ether

28 Office of Biosafety CSP-001 Version Rev Chemical Safety Program February October Page of Group 6: continued Ethylene glycol monomethyl ether Furfuryl alcohol Glycerine Heptanol Hexanol Hexylene glycol Isoamyl alcohol Isooctyl alcohol Methyoxytriglycol Methyl alcohol Methylamyl alcohol Molasses, all Nonanol Octanol Pentadecanol Polypropylene glycol methyl ether Propyl alcohols (n, iso) Propylene glycol Sorbitol Tetradecanol Tetraethylene glycol Tridecyl alcohol Triethylene glycol Undecanol Group 7: Aldehydes Acetaldehyde Acrolein (inhibited) Butyraldehyde (n, iso) Crotonaldehyde Decaldehyde (n, iso) 2-Ethyl-3-prolylacrolein Formaldehyde solutions Furfural Hexamethylenetetramine Isooctyl aldehyde Methyl butyraldehyde Methyl formal Paraformaldehyde Valeraldehyde Group 8: Ketones Acetone Acetophenone Camphor oil Cyclohexanone Diisobutyl ketone Isophorone Mesityl oxide Methyl ethyl ketone Methyl isobutyl ketone Group 9: Saturated Hydrocarbons Butane Cyclohexane Ethane Heptane Hexane Isobutane Liquified natural gas Liquified petroleum gas Methane Nonane n-paraffins Pentane Petrolatum Petroleum ethers Petroleum naphtha Polybutene Propane Proplyene butylene polymer Group 10: Aromatic Hydrocarbons Benzene Cumene p-cymene Coal tar oil Diethybenzene Dodecyl benzene Dowtherm Ethylbenzene Naphtha, coal tar Naphthalene (includes molten) Tetrahydronaphthalene Toluene Triethyl benzene Xylene (m-, o-, p-) Group 11: Olefins Butylene 1-Decene Dicyclopentadiene Diisobuytlene Dipentene

29 Office of Biosafety Chemical Safety Program Group 11: continued 1-Docene Ethylene Liquefied petroleum gas 1-Heptene 1-Hexane Isobutylene Nonene 1-Octene 1-Pentene Polybutene Propylene Propylene butylene polymer Propylene tetramer (dodecene) 1-Tetradecene 1-Tridecene Turpentine 1-Undecene Group 12: Petroleum Oils Asphalt Gasolines Casingead Automotive Aviation Jet Fuels JP-1 (Kerosene) JP-3 JP-4 JP-5 (Kerosene, heavy) Kerosene Mineral spirits Naphtha (non-aromatic) Naphtha Solvent Stoddard solvent VM&P Oils Absorption oil Clarified oil Crude oil Diesel oil Fuel oil No. 1(Kerosene) No. 1-D No. 2 No. 2-D No. 4 No. 5 No. 6 Lubricating oil Mineral oil Mineral seal oil Motor oil Penetration oil Range oil Road oil Spindle oil Spray oil Transformer oil Turbine oil CSP-001 Rev. 0 February 2013 Page 29 of 38 Group 13: Esters Amyl acetate Amyl tallate Butyl acetates (n, iso, sec) Butyl benzyl phthalate Castor oil Croton oil Dibutyl phthalate Diethyl carbonate Dioctyl adipate Dioctyl phthalate Epoxidized vegetable oils Ethyl acetate Ethyl diacetate Ethylene glycol monoethyl ether acetate Ethylhexyl tallate Fish oil Glycol diacetate Methyl acetate Methyl amyl acetate Neatsfoot oil Olive oil Peanut oil Propyl acetated (n, iso) Resin oil Soya bean oil Sperm oil Tallow Tanner s oil Vegetable oil Wax, carnauba

30 Office of Biosafety CSP-001 Version Rev Chemical Safety Program February Page 30 of 38 Group 14: Monomers and Polymerizeable Esters Acrylic acid (inhibited) Acrylonitrile Butadiene (inhibited) Butyl acrylate (n, iso) Ethyl acrylate (n, iso) 2-Ethylhexyl acrylate (inhibited) Isodecyl acrylate (inhibited) Isoprene (inhibited) Methyl acrylate (inhibited) Methyl methacrylate (inhibited) o-propiolactone Styrene (inhibited) Vinyl acetate (inhibited) Vinyl chloride (inhibited) Vinylidene chloride (inhibited) Vinyl toluene Isoprophyl ether Ethers (cont) Tetrahydrofuran Group 22: Phosphorous, elemental Group 23: Sulfur, molten Group 24: Acid Anhydride Acetic Anhydride Propionic anhydride Group 15: Phenols Carbolic oil Creosote, coal tar Cresols Nonylphenol Phenol Group 16: Alkylene Oxides Ethylene Oxide Propylene Oxide Group 17: Cyanohydrins Acetone cyanohydrin Ethylene cyanohydrin Group 18: Nitriles Acetonitrile Adiponitrile Group 19: Ammonia Ammonia hydroxide Group 20: Halogens Bromine Chlorine Group 21: Ethers Diethyl ether (ethyl ether) 1,4 Dioxane

31 Office of Biosafety Chemical Safety Program CSP-001 Rev. 0 February 2013 Page 31 of 38 APPENDIX B: Glove Compatibility Chart The following chart is an example of a glove compatibility chart from Ansell. Many manufactures do provide their own glove compatibility charts online. Be sure to use the chart that corresponds to the gloves used in your work area. The link to the PDF version for Ansell is below.

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