CH2MHILL B&W West Valley, LLC

Transcription

1 CH2MHILL B&W West Valley, LLC Ms. Nicole Wright Toxicity Testing Unit New York State Department of Environmental Conservation Division of Water Bureau of Watershed Programs 625 Broadway, 4th Floor Albany, New York AC-EA WR:2012:0030 May SUBJECT: Whole Effluent Toxicity (WET) Testing Reports for the WVDP SPDES Permit No. NY , Outfalls 001 and 007 Dear Ms. Wright: The West Valley Demonstration Project (WVDP) site has completed the first quarterly Whole Effluent Toxicity (WET) Testing as required on page 11 of 32 of the site's SPDES permit (NY ). The WET testing was completed by New England Bioassay, a Division of GZA GeoEnvironmental, Inc., located in Manchester, Connecticut, through a subcontract with TestAmerica, Buffalo, New York. All test results were within permit limits and the results have been certified by the testing laboratory and the permittee. The test summary data sheets can be found on pages 8 and 9 of 53 for outfall 001 and on pages 7 and 8 of 50 for outfall 007. Please note that the daily average flow at the time of sampling for outfall 001 was 183 gpm, and outfall 007 was 4.8 gpm. The WET testing was completed at outfall 001 in February, 2012 with 24-hour composite samples collected on February 5-6, 2012 and on February 8-9, The WET Testing was completed at outfall 007 in March, 2012 with 24-hour composite samples collected on March 8-9, 2012 and on March 12-13, The test species used in both tests were the Ceriodaphnia dubia (Water Flea) and the Pimephales promelas (Fathead minnow) as specified in the permit. It should be noted that for Outfall 001, the test results were considered "invalid" due to the morbidity percentage of species in the receiving waters. However, the effluent species survival results were 100%, indicating no toxicity. Also because of the batch discharge nature of Outfall 001, retesting was not performed. However, this testing will be performed again next quarter. The WET testing was coordinated with routine chemical and physical parameters required by the site's SPDES permit at each outfall, and those results have been reported in there respective Discharge Monitoring Reports (DMRs). Very truly yours, n D. Rendall, Manager egulatory Strategy JDR:DPK:bnj ATTACHMENTS: 1) Analytical Laboratory Report - Whole Effluent Toxicity Testing for Outfall 001 2) Analytical Laboratory Report - Whole Effluent Toxicity Testing for Outfall 007 CHBWV Rock Springs Road West Valley, NY BNJ5530DPK

2 Ms. Nicole Wright -2- cc: M. A. Jackson, NYSDEC-Region 9 DOW E. W. Wohlers, Cattaraugus County Health Department J. M. Wiedman, NYSDEC-Albany DOW J. M. Dundas, DOE-WVDP, AC-DOE M. P. Krentz, DOE-WVDP, AC-DOE M. N. Maloney, DOE-WVDP, AC-DOE L. E Bennett, CHBWV, AC-PRES (Public Reading Room) H. H. Dukes, CHBWV, WV-PL-6 D. P. Klenk, CHBWV, AC-EA J. D. Rendall, CHBWV, AC-EA R. L. Scharf, CHBWV, WV-PL6 A. W. Upshaw, CHBWV,WV-PL6 W. N. Kean, URS SMS, AC-URS Letter Log (B. Jeffery), CHBWV, AC-BUS CHBWV Rock Springs Road West Valley, NY BNJ5530DPK

3 WR:2012:0030 Attachment 1

4 Tat el. THE LEADER IN ENVIRONMENTAL TESTING ANALYTICAL REPORT TestAmerica Laboratories, Inc. TestAmerica Buffalo 10 Hazelwood Drive Amherst, NY Tel: (716) TestAmerica Job ID: TestAmerica Sample Delivery Group: 93 Client Project/Site: West Valley SPDES CH2M Hill B&W West Valley (CHBWV) MS-ELAB West Valley, New York Attn: Chester Wrotniak Authorized for release by: 4/26/2012 2:31:08 PM John Schove Project Manager I john.schove@testamercainc.com LINKS- Review your project results through TotdAccess Have a Question? Ask The Ex e rt Visit us at: The test results in this report meet all 2003 NELAC and 2009 TN! requirements for accredited parameters, exceptions are noted in this report. This report may not be reproduced except in full, and with written approval from the laboratory. For questions please contact the Project Manager This report has been electronically signed and authorized by the signatory. Electronic signature is intended to be the legally binding equivalent of a traditionally handwritten signature. Results relate only to the items tested and the sample(s) as received by the laboratory. Page 1 of 53

5 Client: CH2M Hill B&W West Valley (CHBWV) TestAmerica Job ID: Project/Site: West Valley SPDES SDG: 93 Table of Contents Cover Page I Table of Contents 2 Case Narrative 3 Subcontract Data 4 Page 2 of 53

6 Case Narrative Client: CH2M Hill B&W West Vane (CBWV) TestAmerica Job ID: Project/Site: West Valley SPDES SDG: 93 Job ID: Laboratory: TestAmerica Buffalo Narrative Job Narrative Comments This report has been revised to correct typographical errors. Chronic Toxicity was performed by New England Bioassay. Final results are contained under this cover. Receipt No analytical or quality issues were noted. Page 3 of 53 TestAmerica Buffalo

7 New England Bioassay, a Division of GZA CeoEnvironmental, Inc. coin t New England Bioassay a DIvI&on ci OZAGooEnvIrn~~e~iai, Inc. CI~OMC AQUATIC TO2UCITY TEST REPORT West Valley Demonstration Project 77 Barson Drive West Valley, New York Manchester, CT Receiving Water: Franks Creek NPDES Permit: NY FAX Test Staff Date: Febniary 7,2012 Test Period: February 2012 Report Prepared by: New England Bioassay A Division of New England Bioassay 77 Batson Drive Manchester, CT NEB Project Number: Report Date: February 24, 2012 Report Submitted to: Test America, Buffalo 10 I-Iazelwood Drive Amherst, NY Sample TD: Outfall 001 Please contact the Lab Supervisor, Kim Wills, at (860) or kimber1y.willsça~gza.com if you have any questions concerning these results. ~ CZA GcoEnvironrncntal, Inc. ww~aga corn Page 4 of 53 An EqS OppaTWiq, Pniptqt MWN4I

8 Whole Effluent Toxicity Testing Report Illstruction Form Client Name/Project:Test America/West Valley Demonstration Project Test Date: 2/7/12 Sample rn Outfall 001 Effluent Your results were as follows: Passed Acute Limits a Fail Please proceed according to the instructions in your permit.,,½ Chronic Limits Invalid see comments section of this report o Original Test Invalid Valid retest performed. Both test and retest results are attached. o Retesting will be or has been perfonned according to the Case I Protocols outlined in the attached copy of EPA-New England s species-specific, self-implementing policy for alternate dilution water. o This is your case of dilution water toxicity. Please proceed according to the Case 2 Protocols outlined in the attached copy of EPA-New England s species-specific, self-implementing policy for alternate dilution water. The alternate dilution water you select for future tests for this species should be described as follows: synthetic laboratory water made up according to EPA s toxicity test protocols, by adding specified amounts of salts into deionized water in order to match the hardness of our receiving water. Writing this letter should help you to avoid retests in the future. o Available information is insufficient to determine whether this test passed or failed. Please compare results to your permit limits. Please submit a current copy of your pernilt to the NEB Lab so that we can determine the status of future tests results and help ensure your compliance with permit requirements. Please complete the items on this list before reporting these results according to the instructions in the Monitoring and Reporting Section of your permit. Please complete, sign and date the upper portion of the Whole Effluent Toxicity Test Report Certification page which is the page directly following this page. Fill in the Sample Type and Sample Method (upper right) and the Permit Limits (lower left) on the New England Bioassay, Inc-EPA Toxicity Test Summary Sheet(s) if they are incomplete. Fill in any missing information on the NEB Chain-of-Custody documents. This includes ensuring that the following information is recorded: Sampler s name and title, Facility name and address, Sample collection methods, Sample collection start and end dates and times, Types of sample, Chlorination status of samples upon shipment to NEB, Site description and Sample collection procedures. Monitoring results should be summarized on your monthly Discharge Monitoring Report Form. Signed and dated originals of this report must be submitted to the State (and Federal) Agencies specified in the Monitoring and Reporting section of your permit. Questions? Please contact the Lab Supervisor, Kim Wills, at (860) or kimberly.willsqi~gza.com, PageS of 53

9 AQUATIC TOXICITY TEST SUMMARY Client: Sample TI): NPDES Number: West Valley Demonstration Project Outfall 001 and Frank s Creek NY NEB Project Number: 05, NEB Test Numbers: A&B Sample Dates: 2/5-6/12, 2/8-9/12 (Effluent) 2/6/12, 2/9/12 (Receiving Waler) Test Dates: 2/7-14/12 Test Type: Test Duration: Test species: Dilution Water Source: Modified Chronic Static Renewal Freshwater Test 8 days (Ceriodaphnia) & 7 days (Piinephales) Ceriodaphnia dubia and Fimephalespromelas Frank s Creek Test Method: U.S. Enviromnental Protection Agency (EPA) Document EPA-82 l-r , Short-Terni Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms, Fourth Edition Concentrations Tested: 0%, 6.25%, 12.5%, 25%, 50% and 100% effluent Acute Results: Ceriodaphnia dithia 48-hour LC5O >100% Pimephalespromelas 48-hour LC5O >100% Ceriodaphnia dubia TUa = 0.3 Pimep hales promelas TUa = Chronic Results: Ceriodaphnia dubia C-NOEC = 100% see comjnen~ section of report Pirneph ales prornelas C-NOEC 100% see comments section of report Ceriodaphnia clubia TUe = j~ see comments section of report Pirnephales promelas TUc j,qj see comments section of report Mean Control Survival: Ceriodaphnia dubia IQQYQ (laboratory control) and loo% (dilution water) Pimephalespromefas Øaboratory control) and 7~~o (dilution water) Reference Toxicant Data: Date: 2/15/12 & 2/1/12 (~eriodayhnia dubia and Pimeyhales yivrnelas) Toxicant: Sodium chloride Source: New England Bioassay C-NOEC: Ceriodaphnia dubia j~gll Pirnephalesproinelas- 0.5 gil Page 6 of 53

10 WHOLE EFFLUENT TOXICITY TEST REPORT CERTIFICATION (Permittec) I certi~ under penalty of law that this document and all A1TACHMENTS were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, tme, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Executed on [Date] (A&thbrized Signature] Bryan C. Bower, Director [Print or Type Name and Title] West Valley Demonstration Project [Print or Type the Permittee s Name] NY [Print or Type the NPDES Permit No.] Since the WET test and report check is complicated, the New England Bioassay Aquatic Toxicity Laboratory has certified the validity of the WET test data in the section below. Please note that this does not relieve the permittee from its responsibility to sign and certify the report under 40 C.F.R. S (k). WHOLE EFFLUENT TOXICITY TEST REPORT CERTIFICATION (Bioassay Laboratory) I certi~ under penalty of law that this document and all ATTACHMENTS were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fme and imprisonment for knowing violations. Exec~edon * 4 [Date [Au o Signature] Kim Wills, Laboratory Supervisor [Print or Type Name and Title) New England Bioassay [Print or Type Name of Bioassay Laboratory] 24. Telephone Contacts If you have questions, please contact Joy Hilton, Water Technical Unit, at (617) or David McDonald, Ecosystem Assessment Unit, at (617) Page 7 of 53

11 NEW ENGLAND BIOASSAY, A DIVISION OF GZA GEOENVIRONMENTAL, INC. EPA TOXICITY TEST SWvIMARY SHEET Facility Name: West Valley Demonstration Project NPDES Permit Number: NY Test Start Date: 2/7/12 PipeNumber: Outfall 001 Test Type Acute Chronic ~ Modified (chronic reporting acute values) 24hr screening Test Species Fathead Minnow X Ceriodaphnia Daphnia Pulex Mysid Shrimp Sheepshead Menidia Sea Urchin _Champia Selenastrum Other Sample Type Prechiorinated Dechlorinated Chlorine Spiked in Lab Chlorinated on site X Unchlorinated TRC: mg/l Sample Method Grab X Composite Flowthru Other Dilution Water X receiving water collected at a point upstream of or away from the discharge, free from toxicity or other sources of contamination; ~.eceiving water name: Franlc s Creelc) alternate surface water of known quality and a hardness, etc. to generally reflect the characteristics of the receiving water; (Surface water name: synthetic water prepared using either Millipore MilI-Q or equivalent deionized water and reagent grade chemicals; or deionized water combined with mineral water; or artificial sea salts mixed with deionized water; deionized water and hypersaline brine; or other Effluent sampling date (s): 2/5-6/12 2/8-9/12 Effluent concentrations tested (in%): * Permit limit concentration: TUa<0.3,TUc<1.O Was effluent salinity adjusted? No Actual effluent concentrations tested after salinity adjustment (%): Q ~ Reference Toxicant test date: 2/15/12 Test Acceptability Criteria Mean Conh ol Survival: Mean Diluent Survival: 100% 100% Mean Control Reproduction: 10.4 young/female Mean Diluent Reproduction: 7.1 young/female LC5O TUa A-NOEC C-NOEC TUc 1C25 1C50 Limits N/A 0.3 N/A N/A 1.0 N/A N/A Results LC5O >100% Upper Value Lower Value 100% Data Analysis Method Used Granhical TUa 0,3 A-NOEC 100% C-NO BC 100%* LOEC >l00%* TtJc 1.0* 1C25 >100%* ICSO Page 8 of 53

12 NEW ENGLAND BIOASSAY, A DiVISION CF OZA OBOENVEiONMENTAL, INC EPA TOXICITY TEST SUMMARY SHEET Facility Name: West Valley Demonstration Project NPDES Permit Number: NY Test Start Date: 2/7/12 PipeNumber: Outfall 001 Test Type Acute - Chronic ~ Modified (chronic reporting acute values) 241w screening Test Species X Fathead Minnow Ceriodaphnia Daphnia Pulex Mysid Shrimp Sheepshead Menidia Sea Urchin _Champia Selenastrum Other Sample Type Prechlorinated Dechlorinated Chlorine Spiked in Lab Chlorinated on site X Unchlorinated TRC: mg/l Sample Method Grab ~ Composite Flowthru Other Dilution Water ~ receiving water collected at a point upstream of or away from the discharge, free from toxicity or other sources of contamination; (Receiving water name: Frank s Creek) alternate surface water of laiown quality and a hardness, etc. to generally reflect the characteristics of the receiving water; (Surface water name: synthetic water prepared using either Millipore Mill-Q or equivalent deionized water and reagent grade chemicals; or deionized water combined with mineral water; or artificial sea salts mixed with deionized water; deionized water and hypersaline brine; or other Effluent sampling datc (s): 2/8-9/12 2/5-6/12 Effluent concentrations tested (in%): * Permit limit concentration: TUa 0.3. TUe 1,0 Was effluent salinity adjusted? No Actual effluent concentrations tested after salinity adjustment (%): Reference Toxicant test date: 2/1/12 Test Acceptability Criteria Mean Control Survival: 100% Mean Diluent Survival: 75% Mean Control Weight: 0.516mg Mean Diluent Weight: mg LC5O TUa A-NOEC C-NOEC TUc 1C25 ICSO Limits N/A 0.3 N/A N/A 1.0 N/A N/A LC5O Upper Value Lower Value Data Analysis Method Used TLJa A-NOEC C-NOEC LOEC TUe 1C25 ICSO Results >100% +co 100% Graphical % loo%* >loo%* 1.0* >loo%* Page 9 of 53

13 CERIODAPHNIA DUBL4 AQUATIC TOXICiTY TEST REPORT Test Reference Manual: EPA 821 -R , Short-Term Methods for Estimating the chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms, Fourth Edition Test Method: Ceriodaphnia dubia Survival and Reproduction Test Test Type: Modified chronic Static Renewal Freshwater Test Temperature: 25 ± 1 C Light Ouality: Photoperiod: Test Chamber Size: Test Solution Volume: Ambient Laboratory illumination 16 hours light, 8 hours daric 30 ml Minimum 15 ml Renewal of Test Solutions: Daily, using most recently collected sample Age of Test Organisms: Less than 24 hours Number of Neonates Per Test Chamber: 1 Number of Replicate Test Chambers Per Treatment: 10 Number of Neonates Per Test Concentration: 10 Feeding Regime: chamber daily. Aeration: Dilution Water: Fed 0.1 ml each of YCT and algal suspension per exposure None Frank s Creek Alternate Control Water: NEB Moderately Hard Water (hardness mg/l) Effluent Concentrations: 0%, 6.25%, 12.5%, 25%, 50% and 100% effluent Test Duration: Until 60% of control females have three broods - 8 days End Points: Survival and reproduction. Test Acceptabili. Control Survival:? 80% Yes X No Control Reproduction: Average? 15/control female Yes No ~ Sampling Requirements: NYSDEC has approved West Valley Demonstration Proj ect to use only two sets of samples for their chronic testing due to the batch nature of their discharge. Sample Volume Required: Minimum 2 liters/day Test Organism Source: NEB Page 10 of 53

14 Test Acceptability Criteria:Mean Alternate Water Control Survival 100% Mean Dilution Water Control Survival 100% Mean Alternate Control Reproduction = 10.4 I surviving female Mean Dilution Control Reproduction = 1.1/ surviving female Test Results: Limits Results 48-hour LC5O >100% Upper Value + ~ LowerValue 100% Data Analysis Method Used Graphical A- 100% TUa hour ECSO >100% Survival C-NOEC 100% Reproduction C-NOEC 1 00%* Reportable C-NOEC l00%* TUe * LOEC >100%* MAWC >100%* 1C25 > 100% ~ Reference Toxicant Data: Date: 2/15/12 Toxicant: Sodium chloride Dilution Water: NEB Lab Synthetic Soft Water Source: New England Bioassay IC~: WL In Acceptable Ran2e: Yes X No Dechlorination Procedures: Chlorine is measured using 4500 CL-G DPD Colorimetric Method. ~ Dechlorination was not required Sample was dechlorinated by adding sodium thiosulfate to the sample prior to test initiation. Since dechlorination of the effluent was necessary, a thiosulfate control of dilucnt water spiked with sodium thiosulfate was also included in the test series. Chlorine was mg/l in a dechlorinated sample. Chlorine Measurement was elevated due to interference, Chlorine was mg/l in a filtered sample. Total Residual Chlorine was re-measured following aeration, and was found to be mg/h Page ii of 53

15 PIMEPHALES.PROMELAS AQUATIC TOXICITY TEST REPORT Test Reference Manual: EPA 821 -R-02-0l 3, Short-Term Methods for Estimating the chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms, Fourth Edition Test Method: Pimephales promelas Larval Survival and Growth Test Test Type: Modified Chronic Static Renewal Freshwatcr Test Temperature: 25 ± 1 C Light Quality: Photoperiod: Test Chamber Size: Ambient Laboratory Illumination 16 hours light, 8 hours dark 600 ml Test Solution Volume: Minimum 200 ml, Renewal of Test Solutions: Daily, using most recently collected sample Age of Test Organisms: Less than 24 horns Number of Larvae Per Test Chamber: 10 Number of Replicate Test Chambers Per Concentration: 4 Number of Larvae Per Test Concentration: 40 Feeding Regime: Cleaning: Fed 0.1 ml newly hatched brine shrimp nauplii twice daily. Siphoned daily, immediately before test solution renewal. Aeration: DO concentration fell below 4.0 mg/l Yes No ~ Aerated at <100 bubbles/minute Yes No X Dilution Water: Frank s Creek Alternate Control Water: NEB Moderately Hard Water (hardness mg/l) Effluent Concentrations: Test Duration: End Points: 0%, 6.25%, 12.5%, 25%, 50% and 100% effluent 7 days Survival and growth. Test Acceptability: control Survival:? 80% Yes X No control Average Dry Weight? 0.25 ing Yes ~ No Sampling Requirements: NYSDEC has approved West Valley Demonstration Project to use only two sets of samples for their chronic testing due to the batch nature of their discharge. Sample Volume Required: Minimum 2.5 liters/day Page

16 Test Organism Source: NEB Test Acceptability Criteria:Mean Dilution Water Control Survival = 75% Mean Alternate Water Control Survival = 100% Mean Dilution Water Control Weight = mg Mean Alternate Water Control Weight = mg Test Results: Limits Results 48-hour LC5O >100% Upper Value +~ Lower Value 100% Data Analysis Method Used Graphical A-NOEC 100% TUa hour ]EC5O >100% Survival C-NOEC l00%* Growth C-NOEC 100%* Reportable C-NOEC l00%* TUe * LOEC >loo%* MAWC >loo%* 1C25 >100%* Reference Toxicant Data: Date: 2/1112 Toxjcant: Sodium chloride Dilution Water: NEB Lab Synthetic Soft Water Source: New England Bioassay ICE: WL In Acceptable Range: Yes X No Dechlorination Procedures: Chlorine is measured using 4500 CL-G DPD Colorimetric Method. X Dechlorination was not required - Sample was dechlorinated by adding sodium thiosulfate to the sample prior to test initiation. Since dechlorination of the effluent was necessary, a thiosulfate control of diluent water spiked with sodium thiosulfate was also included in the test series. Chlorine was mg/l in a dechlorinated sample. - Chlorine Measurement was elevated due to interference. Chlorine was xng/l in a filtered sample. - Total Residual Chlorine was re-measured following aeration, and was found to be ing/l. Page 13 of 53

17 Additional Notes or Other Conditions Affecting the Test: The EPA acceptability criteria for both the mean number of young per female in the Ceriodaphnia and the survival in the dilution water for the fathead minnows were not met, Normally a retest would be scheduled however Outfall 001 only discharges for 6-7 days at a time, and only at certain points_during the_year. therefore another sample was not able to be obtained at this time. Since resampijug is not an option for West Valley 001 we are reporting the data we collected, and our observations and conclusions based on the results, *CepjodapJl,jja dithia: The survival of the Ceriodaphnia met all validity requirements, so we were able to accurately report the acute data and the survival C-NOEC. The mean total of young per female in both the laboratory control water and the Frank s Creek dilution water was less than 15 per female, which does not meet the EPA s acceptability criteria and therefore statistical analysis is technically not valid for the reproduction data. However when comparing the 100% effluent reproduction (9,3 young/female) to the laboratory water (10.4 younwfemale) it would appear there is no statistically significant decrease. When comparing the 100% effluent to the Franic s Creelc (7.1 young/female) the effluent actually produced a slightly lugher number of young (9,3 per female), indicating that there is no effluent toxicity in comparison to the river. Since the survival data shows no effluent toxicity, and the reproduction data does not indicate any effluent toxicity, we are reporting the TUe as 1.0 for the Ceriodaphnia. *Fathead minnows: The survival data meets all validity requirements at 48-hours therefore the acute endpoints are valid and reportable. The survival in the Frank s Creek was 75% at 7-days, which falls below the 80% validity criteria. The survival in the 100% effluent was 97.5% at test completion, and the lowest survival rate in any effluent concentration was 95% at test completion. When the effluent survival data is run against the laboratory control water, which had a 7-day survival of 100%, there is no statistically significant difference. When looking at the fish weights we see that the mean weight in the 100% effluent was mg, which the statistics show is not significantly decreased from the laboratory control weight of mg. This data strongly concludes that the effluent is not toxic to either the survival or weight of the fathead minnows. and we are reporting a TUc of 1.0. River-Water Toxicity: The phenomenon of river-water toxicity to fathead minnows (but not daphnids) in chronic tests is not uncommon. (Kszos and Stewart. 1992: Grothe and Johnson, 1996; Kszos, 1997; SETAC/EPA WET expert advisory panel, 1999: Downey et al. 2000; Federal Rcgister, 2002). These investigators observed that unacceptable survival of fathead minnows in chronic toxicity tests using receiving water may be associated with a natural pathogen (possibly a bacteria) found in river water. The phenomenon usually has these characteristics: - High variability in fish survival is often observed among replicates within a concentration in receiving water and lower effluent concentrations. - Adverse survival effects are seen in chronic tests with fish but not in chronic tests with daphnids:_adverse_effects_are usually not observed in acute fish tests. - Mortality_is usually_first_observed on test days 3 or 4; once mortality is noted in an individual replicate, most, if not all, fish in that replicate will succumb beforc the test is completed. - A fiangal growth is sometimes observed on the fish; the fungal growth has been attributed to Saprolegnia vp. and appears to be a secondary infection. - Page 14 of 53

18 NEW ENGLAND BIOASSAY TOXICITY DATA FORM CHRONIC COVER SHEET CLIENT: ADDRESS: CONTACT: Test America. Inc. 10 Hazelwood Drive Amherst. NY West Valley C dubiatestidno: ~a ~I1~ ~ 1. promelas TEST ID NO: LQ -a7& lb COCNO: ~~)qljq-/l~~ PROTECT NO: a~- Ctqq~3qo co SAMPLE TYPE: DILUTION WATER SOURCE: Receiving Water INVERTEBRATES VERTEBRATES TEST SET UP (TECH INIT.): ft\gi TEST SET UP (TECH INIT.): Al 1/ TEST SPECIES: Ceriodcphnia dtthia SOURCE/NEB LOT#: ~d l,q (-~O3o ~ AGE: <24 Hours TEST SOLUTION VOLUME: 15 ml NO. ORGANISMS PER TEST CHAMBER: I NO. ORGANISMS PER CONCENTRATION: 10 START DATE: END DATE: AT ~-j)~5i I a AT l~o~ hours IH~hours TEST SPECIES: Pi,neohales pronielas SOURCE/NEB LOT #: Pp/V a-?) AGE: <24 Hours TEST SOLUTION VOLUME: 400 ml NO. ORGANISMS PER TEST CHAMBER: 10 NO. ORGANISMS PER CONCENTRATION: 40 START DATE: - END DATE: 7/7/Il Dli z.~( ~- AT IZU>- hours AT l~-~o hours Laboratory Control Water Test Batch Hardness Day Number mg/i CaCO3 Alkalinity mg/i CaCO3 Sample Collection Time Effluent# I from: P-/3ll~ at to: a/t~li~ at 1500 Diluent# I ailo(t? at pqao _Q-~ Q~h%W~o) ~ c1a ~ ~~~ I i o~-mi&~ ~(s j_ C*4v\kcoZ- qq _i_ O3~nu~vo.~ fr\\1ft~ Q4 6 cn~ivntoa ~7 ew-p4*~z 9 (00 (oo LcD LoS (Os (05 (05 Effluent#2 from: 2)8112 at l0~-0 to: L~l9iW at l0~b Diluent#2 to: M9/S.2 at Effluent#3 from: N at to: MI. at Diluent#3 to:jsj_& Results of Ceriodayhnia dubia Chronic Test 95%Conf. Umit 48 Hour LC50: 7DayLC50: >~otl kto~ Survival NOEC: (0 0 Survival LOEC: > I Reproduction NOEC: Reprcduction LOEC: > 1063) Reproduction 1C25: NOEC: NO-OBSERVEO-FFFECT-CONCENTRATION Results of P/rn ephales oronielas Chronic Test - 95% ConE Linut 48 I-IourLC50: ita/ 1~t~ - 7DayLC~o: lo)/ rn ±00 Survival NOEC: * Survival LOEC: 7 10)/. - Grow Eli NOEC: Growth LOEC: -? ~ 4 W a? Growth 1C23: ico/ -~ LOEC: LOWEST-OBSERVED-EFFECT-CONCENTRATION ANThVIAL CONDITION/BEHAVIOR: COMMENTS: ~ 5pQ Ct~mr w,~\5 SE&1CYP\ crc ccqoc4 REVIEWED BY: LjUj ()S4~r C7/ Page 15 of 53 DATE

19 NEW ENGLAND BIOASSAY- CHRONIC TOXICITY TEST BROOD DATA SHEET Pagel ofl FACIlItY NAME & ADDRESS: West Valley Demonstration ProjectWSNPOOl, Rock Springs Rd West Valley, NY NEB PROJECTNU?vIBER:05, ODc4~I ~L1o INEB TESTNUMBER:j~ c.qfls<fl CO t~n~_iljt.1l.j 1-is TEST ORGANISM: Cefloclczphnla dubia I AGE: <24 hoots LOT #: ~, d a(~hh ~ IT1ME \~_r~e5 ENDDATE: IT~5 JL/0Q STARTUATE: 61~ 1 1 I ~- Effluent Day Source I: Snuice 2: Total Number Analyst - Analyst - concentration Number Live Live Transfer Counts Young Adults Replicate AB C D H F 0 H I ~ vw~ -~ I I [it AW ~i I ~J 1 I 1 (X3 ~) NEBLsb ~ o ~ e~ e~ Synthetic 4 ~ I ~ij t ~ j t ItO (ItS Control 5 ~f j J_ ~J -/ ~ 4 V 3 j ~ 0 6L3_ ~- u ~~ ~ flaivmt ~ V) /41/ ~nj 2 v it) W~ 2 1Y7C~ Totals 0 o ~i ~ q I I I I q T V vv V/j7~L p io Receiving ~ 2. 7~ V2~ Z4 liz V I ~3 up L O Water v ~J / T- (9 Diluent 6 ~z ~Z~IY~I 1 ~ TT77~T I ~ I j~v S blab 1~ ~o 0 ~! ~ J q T Un - ~ 6.25% 4 {vvv~tv ~ ~,- o L o 5 6 ~ L..- t~jzt~&~7_. (j3 7 j~fl_2_~ ~ I~LiL 1 botab H In Notes: Page 16 of 53

20 NEW ENGLAND BIOASSAY- CIIRONTC TOXICITY TEST BROOD DATA SHEET Page 2 of ~ FACILITY NAME & ADDRESS: West Valley Deinonstnition Pioject WSNPOOI, Rock Splings Rd West Valley, NY NEB PROJECTNUMBER: 65.tb4Ll~4D.Pt) ORGANISM: Ceriodaplinia ththia STARTDATE: ~-h I Effluent Day Total Nunibet Analyst - Analyst - concentration Numbei Replicate Live Live Tiansfet Counls ~ Young Adults A B C D B P 0 H I 3 0 ito 1$. 1 r bce I*S~ ~S 2 ~o ~ v ~ 0 Hit i25% 4 7VVThz v 4V~[V 40 ~D IS ~ ~ V to Iflsta 6 10 IW ~ ~ 7 ~Zç -3~_~Li çcc-q 4;t un S~ fl L6~ -~ T~s 1 ~ ~r :1-- ~~ Zr ~T r Zr ~r r ~:0 I 2 T Zr Zr T ~ ~ io I~, 3~ % 4 ~~ ~ 0 io ~ II ~ 6 E ~- ~ (0 ~ ~ t7~2~ L g~a t±~~-q ~1 ~ T~&s I ~ -T----r Va 2 flt~~.i 50% : - ~ km 100% ~ 6 ~ ~ W5 ~1 i t39 7~-G~rO 0?4, ~ ~ ~ a Toh~ 0 I I T T T Y~ g Zr I 2~ 4 ~ g ~ ~ i2~l2~& ~ 0 6 7Z ~ 9 9 ~ t ~3-5 -!~1_9~~ 2 ~ i_ex_ ~n_l 93 ~ Tot± ai~&atiilal qs c na Page 17 of 53

21 CT-TON: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,so SN IS NOT CALCULABLE SPEARNAN-KARBER TRIM:.00% LC5O; % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (%) D D D D D 01 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LCSO FOR THIS DATA SET IS GREATER THAN WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE ANY STATISCALLY SOUND RESULTS. DATE: 2/7/12 TEST NUMBER: a DURATION: 48 h SAMPLE: West Valley 001 SPECIES: C.dubia METHOD LC5O CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ******* ~ MAA ******* ******* ******* PROBIT ******* ******* ******* ******* SPEARNAN.000 ~ ~ = LIMIT DOES NOT EXIST Pacie 18 of53

22 CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS MINIMUM REQUIRED TRIM IS TOO LARGE: 80.0,80 8K IS NOT CALCULABLE SPEARMAN-KARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (%) D O D D+O D D+D1 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LCSO FOR THIS DATA SET IS GREATER THAN THE MOVING AVERAGE METHOD CANNOT BE USED WITH THIS DATA SET BECAUSE NO SPAN WHICH PRODUCES AVERAGE ANGLES BRACKETING 45 DEGREES ALSO USES TWO PERCENT DEAD BETWEEN 0 AND 100 PERCENT. NO CONVERGENCE IN 25 ITERATIONS. PROBIT METHOD PROBABLY CAN NOT BE USE WITH THIS SET OF DATA. DATE: 2/7/12 TEST NUMBER: a DURATION: 7 days SAMPLE: West Valley 001 SPECIES: C.dubia METHOD LC5O CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ~ ******* ~ MAA ******* ******* ******* ******* PROBIT ******* ******* ******* ******* SPEARt4AN.000 ******* ~~ ktr ~ NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING. ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION. LIMIT DOES NOT EXIST Paqe 19 of 53

23 CETIS Analytical Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Test Code: 23 Feb-12 15:12 (p1 of 4) a New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CET1SvI.8.4 Analyzed: 23 Feb-12 15:11 Analysis: STP 2x2 Contingency Tables Official Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 07 Feb-12 12:05 Protocol: EPA 821/R-02-0i3 (2002) Dlluent: Ending Date: IS Feb-12 14:00 Species: Cerlodaphnia dubia Brine: Duration: Sd 2h Source: in-house Culture Age: <24h Sample ID: Code: 2A Client: TestAmerica Sample Date: 06 Feb-12 Material: industrial Effluent Project: Receive Date: 07 Feb-I 2 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU Untransformed C >T NA NA 100 >100 NA Fisher Exact/Bonferroni-Holrn Test Control vs C-% Test Stat P-Value P-Type Decision(a:5%) Lab Water Exact Non-Significant Effect Exact Non-Significant Effect Exact Non-Significant Effect Exact Non-Significant Effect Exact Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Control Resp NL Yes Passes Acceptability Criteria Data Summary C-% Control Type NR R NR + R Prop NR Prop R %Effect 0 LabWater I 0 0.0% 6, % ,0% 25 9 I % ID I 0 0.0% -too % 7d Survival Rate Detail C-% Control Type Rep I Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 LabWater I I I I 12.5 I 1 I 1 1 I I I I 50 1 I I I 0 7d Survival Rate Binomials C-% Control Type Rep I Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 Lab Water i/i ui 1/1 In in i/i 1/1 ui in i/i 6.25 in in -i/i in in in in in in in 12.5 in in in in in in in in In in 25 in in in on in in in in in in So in in in in in in in i/i in in 100 in in on in in in in in on ui CrIS~ vi,8.424 Analyst: QA: Paqe 20 of53

24 CETIS Analytical Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Test Code: 23 Feb-12 15:12 (p2 of 4) a New England Bioassay Analysis ID: Endpoint; Td Survival Rate CETIS Version: CETISv1.8.4 Analyzed: 23 Feb-12 15:11 AnalysIs: STP 2x2 Contingency Tables Official Results: Yes Graphics 0.0 C S S 0.5 S 0.0 S 0.? Os a, Os to OS O~ GETIS vi.8*24 Analyst QA: Paqe2l ot53

25 CETIS Analytical Report Report Date: 23 Feb-12 15:12 (p3 of 4) Test Code: a I Ceriodaphnia 7-d Survival and Reproduction Test New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CETISv1.8.4 Analyzed: 23 Feb-12 15:11 Analysis: STP 2x2 Contingency Tables Official Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 07 Feb-12 12:05 Protocol: EPN82I/R-02-0I 3 (2002) Diluent: Ending Date: 15 Feb-12 14:00 Species: Cerlodaphnla dubla Brine: Duration: 8d 2h Source: In-House Culture Age: <24h Sample 113: Code: 2A Client: Test America Sample Date: 06 Feb-12 Material: Industrial Effluent Project: Receive Date: 07 Feb-I 2 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU Untransformed C> T NA NA 100 >100 NA I Fisher Exact/Bonferroni-HOIm Test Control vs C-% Test Stat P-Value P-Type Decision(a:5%) Dilution Water 6.25 I Exact Non-Significant Effect Exact Non-Significant Effect 25 0_S Exact Non-Significant Effect Exact Non-Significant Effect ,0000 Exact Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Control Resp NL Yes Passes Acceptability Criteria Data Summary C-% Control Type NR R NR + R Prop NR Prop R %Effect 0 Dilution Water % % % % % % 7d Survival Rate Detail C-% ControlType RepI Rep2 Rep3 Rep4 Rep5 Rep6 RepT RepS Rep9 ReplO 0 DilutlonWater I I I I I I I I ld Survival Rate Binomials C-% ConirolType Repi Rep2 Rep3 Rep4 Rep5 RepG Repl RepS Rep9 ReplO 0 DIlution Water 1)1 1/1 1/i 1/1 1/1 1/1 1/1 1/1 1/1 1/ /1 1)1 1/1 in in in in in in in /1 1/1 1/1 in in in in in ui in 25 1/1 1/1 1/1 Wi 1/1 1/1 1/1 1/1 ui in 50 in in in 1/i In in ill in i/i in 100 in in on In in i/i In in on in CETIS TM vi Analyst: Paqe 22 of53

26 CETIS Analytical Report Cerlodaphnia 7-d Survival and Reproduction Test Report Date: Test Code: 23 Feb-12 15:12 (p4 of 4) a I New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CETISvL8.4 Analyzed: 23 Feb-12 15:11 Analysis: STP 2x2 Contingency Tables Official Results: Yes Graphics IS C S 05 C 0.I S.7 as 0, , IS, GETIS vi.6.4,24 Analyst: Page 23 of 53

27 CETIS Analytical Report Report Date: 23 Feb-12 15:12 (p1 of 4) Geriodaphnia 7-cl Survival and Reproduction Test Test Code: Analysis ID: Endpoint: Reproduction CETIS Version: CET1SvI.8.4 Analyzed: 23 Feb-12 15:11 Analysis: Parametric-control vs Treatments Official Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 07 Feb-12 12:05 Protocol: EPN82I/R (2Q02) Diluent: Ending Date: 15 Feb-12 14:00 SpecIes: Ceriodaphnladubla Brine: Duration: 8d 2h Source: In-House culture Age: <24h Sample ID: Code: 2A Client: Test America Sample Date: 06 Feb-12 Material: Industrial Effluent Project: Receive Date: 07 Feb-12 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Now England Bioassay Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Untransformed NA C> T NA NA 100 >100 NA % Dunnett Multiple Comparison Test Control vs C-% Test Stat Critical MSD DF P-Value P-Type Declsion(o:5%) Lab Water cdf Non-Significant Effect 12,5 1, CDF Non-Significant Effect COF Non-Significant Effect CDF Non-Significant Effect , CDF Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Control Resp NL Yes Below Acceptability Criteria PMSD Yes Passes Acceptability Criteria - ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(o:5%) Between Non-Significant Effect Error , Total Distributional Tests Attribute Test Test Stat Critical P-Value Decislon(a:1%) Variances Bartlett Equality of Variance Equal Variances Distribution Shapiro-WlIk W Normality 0, Normal Distribution Reproduction Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max Std Err CV% %Effect 0 Lab Water % 0.0% , % 12.5% 12, , % 21.15% % 24.04% as % 5.77% % 10.58% Reproduction Detail a C-% ControiType Repi Rep2 Rep3 Rep4 RepS RepS Rep7 RepB Rep9 ReplO 0 Lab Water IS CETISTh vi Analyst QA: Page 24 of 53

28 CETIS Analytical Report Ceriodaphnia 7-d Survival and Reproduction Te5t Report Date: Test Code: 23 Feb-12 15:12 (p2 of 4) a New England bioassay Analysis ID: Endpoint: Reproduction CETIS Version: CETISvI.8.4 Analyzed: 23 Feb-12 15:11 AnalysIs: Parametric-Control vs Treatments Official Results: Yes Graphics S. I I H -a IZO SD l.s -SD s.o as CETIS TM vi Analyst: QA: Page 25 of 53

29 CETIS Analytical Report Report Date: 23 Feb-12 15:12 (p3 of 4) Test Code: a I Ceriodaphnia 7-d Survival and Reproduction Test Analysis ID: Endpoint: Reproduction CETIS Version: CETISvL&4 Analyzed: 23 Feb-12 15:11 Analysis: Parametric-Control vs Treatments OffIcial Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 07 Feb-12 12:05 Protocol: EPN82I/R (2002) Dlluent: Ending Date: 15 Feb-12 14:00 SpecIes: Cerlodaphnla dubia Brine: Duration: 8d 2h Source: In-House Culture Age: <24h Sample ID: Code: 2A Client: Test America Sample Date: 06 Feb-12 Material: Industrial Effluent Project: Receive Date: 07 Feb-I 2 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: New England Bioassay Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Untransformed NA C >T NA NA 100 >100 NA % Dunnett Multiple Comparison Test Control vs C-% Test Stat Critical MSD DF P-Value P-Type Decision(o:5%) Dilution Water CDF Non-Significant Effect i CDF Non-Significant Effect CDF Non-Significant Effect CDF Non-Significant Effect CDF Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Control Resp NL Yes Below Acceptability Criteria PMSD Yes Above Acceptability Criteria ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(a:5%) Between Non-Significant Effect Error Total Distributional Tests Attribute Test Test Stat Critical P-Value Decision(a:1%) Variances Bartlett Equality of Variance Equal Variances Distribution Shapiro-Will< W Normality Normal Distribution Reproduction Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max SW Err CV% %Effect 0 Dilution Water % 0,0% % % _S % % % -1127% % % ,54% % Reproduction Detail C-% Control Type RepI Rep2 RepS Rep4 Rep S Rep 6 Rap? RepS Rep9 ReplO 0 Dilution Water , CETIS ~ vi Analyst: QA: Page 26 of 53

30 CETIS Analytical Report Ceriodaphnia 7-d Survival and Reproduction Test Report Date: Test Code: 23 Feb-12 15:12 (p4 of 4) a New England Bioassay Analysis ID: Endpoint: Reproduction CETIS Version: CET1Sv1.8.4 Analyzed: 23 Feb-12 15:11 Analysis: Parametric-Control vs Treatments Official Results: Yes Graphics 52 I I H.~ 0.JotO Olsi H J _ I I I I s so skit, GETISTh vi Analyst QA: Page 27 of 53

31 NEJfl 13KM SHEETFOR ROUTJYE CHEMICAL AND PHYSICAL DETERMINATIONS Pn~e I of 2 FACIIJTYNAfvIE & ADDRESS: West Valley Deinonsuation Project WSNPO0I, [0282 Rock Springs Rd West Valley, NY NED PROJECT NUMBER: ~ e. cu-(4 a4o DO TEST ORGANISM: Ceriodaphnia duhia DILUTION WATER SOURCE: Receiving water START DATE: 2 (-7 TIME: J,,) 05 ANALYST fri REMARKS NEBLab Synthetic Control Ternp~ C Initial z4l~ & f;li 7~i3 ~E-O ~ p ZY4 ~ ~4f D.O~mg/L Initial ga. ~5/ Th0 7-7 ~ ~ ç pii,s.tl Initial ~ ~ 7~7?R 74D.7- Y Tq 77 CondnctivibjjLS Initial ~7 \ 3p~ 31~ ~qg~ ~p_i~ ~ t~ 3 o~s Temp., C Final ZLIM ~ ~ Vto al-f C) &~~? á~4) ~?47 D.O.,mgJL Final ~.5 c~i~ 9q3 ~ 7,7 ~5 94 ph, au. Final - 7~ ~13 ~>I 84 7~? 71~ 7 L7 Conduutivi~ ~ts Final ~ 350 3(~3 ~CO 36? ~ ass Receiving Water REMARKS Diluent Temp., C Initial 240,~ o c 2- ( , 0 21/ - 0 y o21q~u D.O~rnWL Initial jq~ ~16 qn 8:1 3-3 ~ 79 ph,&tt Initial ~ H 7~3 ~.Z ~,n~ Conductivity~S Initial ~O~- 1g3 43 ~-ii- zn- z-j ~,7 ~- Temp., C Final ~ ~2 V(1~l 21-hG ~i-~ &~ D.O~mg/L Final % 9-f ~ ZR ph,s.u. Final So ~ s--i?-3?~o q,q gj Conductivity, ~ts Final zz I S? -16 c$-l- I 6,25% REMARKS Teinp., C Initial ~ ~2 Fo ~ 7q~-p Z -~ ~O 246 C~5L8 ~ô D.O~mg/L Initial L4-3 gq )z2?a ~? pl~s.u. Initial 7~ 7(4 9~. / Conductivi~S Initial ~n in vi (~ 310 2ñ(c ~7 ~2~9 Temp., C Final ~ 2Lf.5 ~L}.j 3N-O ZLt~O 2* (~ ov 12 ~fl3 D.O.,ing/L Final g.~ q,(0 M ~-,q- 7~5 717 q~l ph,s.u. Final Z~o ~a Th~) ~-,3 ~ ) Conductivit~S Final ZQY,2t~9 3O~[ 3~~- jq5 C295,,,~]7 12.5% I REMARKS Temp C Initial 2N& ~4D z~ ZJfr6 2~-hO z t-o 2c~.5th?) D.O.,mg/L Initial ~4 q q1tl Q -9 ~7. 2 Q-~ 3,3 ~-/ ph,s.u. Initial 7i1 qjf 75?t J 7-1 -; - I tt~lf q 4 Conductivity, ILS Initial ~ ~3gl~o z~3 cqts Temp C Final 241 Q~ &*3 Z (- I 2H-~()?jv3 2i~ ~ D.O,,rng/L Final ~3 9~ ~[3 ~3 77 7,~- 94 ph,sat Final ~ ~ a--i ~3 Conductivity, jis Final ~ 7 39 i 2c4 Lto ~ Q~ 3~ 3t4: Page 28 of53

32 NED ½ DATA SHEET FOR ROUTINE CHEMICAL AN!) PHYSICAL DETERMINATIONS Page~ of ~2 FACILITY NAME & ADDRESS: West ValleyDemonslmtionPtoject WS?~P00l, Rook Springs Rd West Valley, NY NEB PROJECTNUMBER: b5. Db ~NQ4O TEST ORGANISM: Ceriodaplmia dunn DILUTION WATER SOURCE: Receiving Water 25% Ternl~. C Initial z~ 1c ~24. ~ 2Li to zc t ~o Z -t~o 2~-O a3g ~t~t D.O.,mg/L Initial q3 z&-j ~s ~ ph,s.u. Initial z~ 7~c 7~I 7~~a. 7cf 7~fr ic Conductivity, ~LS Initial ç. ~çt{fr-~ 5~p ~ z ~ Teinp., C Final ~ ~i~zj ~i44 rn?1 2q,~ c~ 3 ~2qi p 02 {s D.O,,rng/L Final Si CBs ~ I o 3~ ph,s.u. Final $~O ~ S~3 &D T9 33 Conductivity, [IS Final ~3 5(jl clad E~+S ~5t~ C5LfI3 ~3 REMARKS 50% REMARKS Temp., C Initial 14~o,~o zft& Vt-b 29:0 z~q.. g 2.44_ >2/aU D.0~ing/L Initial qi3 to-c C:f3?3 8~f3 HO ph,s.k Initial ~ 7(Q 7(7 7~7 ~ 3 ~ ZT tiff Conductivity, p5 Initial ~ç q09 I ~q F?5~e a-~4 x~4 Teinp., C Final Ztlcf PLL9 ~ ZtC-C 24,0 ~ ~{o ~ D~,mg/L Final 91 ~(3 a~ i 7t~ 7W 9J~ ph,sat Final ~ ~?-q gç5 8~ I Re Conductivity, ~ S Final ~?O 91 & 9O~ 2?<3 1 ~ ass 9 +~ X R 100% REMARKS Temp., C Initial lts~,j., 44~o Zc c 2JE-O 2tg zq-o P -kj ~2lg/3 D.0,,ing/L Initial IICi 9. 7~ 8~C 8~~Z?~ ph,s.u. Initial ~7 ~Y 7~ L?-S Conductivits i~ Initial ~5-ç~( (jqfl~)_ t 2 ~ (Lf53 ~ )J-#1S 429 Temp., C Final ~ ~q~e ~ti~ z~-z- Z -FtO ~+& D25Th ~g;~ D.O.,ing/L Final 3 f g3 10,0 ~-6 ~:3 7 ~p *i 95 pu,a Final ~ g,3 ~ ~a&~ s.d. s.c. xc Conductivity, [ts Final jç-t G 15~a LaG, I I~f z~, 14-3a 14; 8 im~5 /4T~ Temp., C Initial STARTOATE: e-ii I I ~ TIME: ip7j3 S~ I REMARKS DO., mg/i. ptm, sn. Initial Initial Conductivity, gs Initial Temp., C Final 0.0., mgil Final ph, sn. Final Conductivity, ~is Final Page 29 of 53

33 NEB S SURVIVAL DATA SHEET FOR FATI{EAI) MINNOW LARVAL SURVIVAL AN]) GROWTH TEST Page 1 of J FACILITY NAME & ADDRESS: West Valley Demonstration Project WSNPOOI, Rock Springs Rd West Valley, NY NED PROJECTNUMEER O5a~~-Poo INEB TESTNUMBER: ~ ~fl COC#: (29- TEST ORGANISM: Pirnephalespro,nelas AGE: <24 horns LOT#: ~ (~ START DATE: ~\1 \ \> TU~E: END DATE: ~ )~+/L?- Tll~1E: o DO concentration fell below 4.0 mg/l 0 All test solutions were aerated at <100 bubbles/minute as of Number of Survivors Effluent Replicate Day Concentration Number 0 I J 2 3 j 4 J 5 6 ] 7 Remarks ] ANALYST p~1~ ~ NEBLab A 10 I,O (C /p (a) Synthetic B 10 fl IC) to to In [0 Control C 10 lo 10 [0 10 (0 /0 D TO ~ /0 CO ~ 10 (0 Receiving A IC (~ LO \ tb /& /0 Water B 10 I~ 10 O ~ 0 0 ~~-o ~4o3~ th,6&vtc& Genteel C (0 10 1,0 10 /0 Dik 10 ~ to to to //? (C) A IC [0 /o 6.25% B 10 l.a to LO to ( 0 ~ 0 ~ íô to D 10 ~ ( A to ic /0 12.5% B 10 ~3 to 10 to 10 /~ to C 10 1~ ~ to ~ 10 to D 10 \fl to A 10 co 10 to ib 25% B 10 [0 fo C 10 LU 10 io 10 /0 D ID ID 10 IO /p A 10?fl (n to IOjp fn 50% B 10 (~ (p ((9 [0 ~ [V C 10 (f) 10 [0 ~ to / ID io In 10 A 10 go 10 to ie to / 100% B 10 lb 10 jo 10 c C 10 ( ~ 10 to to 10 LV /o Page 30 of 53

34 CT TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,80 SM IS NOT CALCULABLE. SPEARMAN-KARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL % EXPOSED DEAD DEAD PROB. 3) , D D D O95D LO D 10 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LCSO FOR THIS DATA SET IS GREATER THAN WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE ANY STATISCALLY SOUND RESULTS. DATE: 2/7/12 TEST NUMBER: DURATION: 48 h SANPLE: West Valley SPECIES: P. promelas METHOD LC5O CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ******* ~ ~****** MAA ******* ******* ******* ******* PROBIT ******* ******* ******* ******* SPEART4AN.000 ******* ~ LIMIT DOES NOT EXIST Page 31 of 53

35 MINIMUM REQUIRED TRIM IS TOO LARGE: 97.5,80 6K IS NOT CALCULABLE SPEARMAN KARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (%) D D D D D 08 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LC5O FOR THIS DATA SET IS GREATER THAN THE MOVING AVERAGE METHOD CANNOT BE USED WITH THIS DATA SET BECAUSE NO SPAN WHICH PRODUCES AVERAGE ANGLES BRACKETING 45 DEGREES ALSO USES TWO PERCENT DEAD BETWEEN 0 AND 100 PERCENT. NO CONVERGENCE IN 25 ITERATIONS. PROBIT METHOD PROBABLY CAN NOT BE USE WITH THIS SET OF DATA. DATE: 2/7/12 TEST NUMBER: DURATION: 168 h SAMPLE: West Valley SPECIES: P. promelas METHOD LC5O CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ~ ~ 9c~~ ~** MAA ******* ******* ******* PROBIT ******* ******* ******* SPEARMAN.000 ******* ******* ******* NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING. ADJUSTMENTS WERE MADE PRIOR TO SPEABMAN-KARBER ESTIMATION. LIMIT DOES NOT EXIST Page 32 of 53

36 I CETIS Analytical Report Report Date: 23 Feb-12 15:05 (p1 of 4) Test Code: b Fathead Minnow 7-d Larval Survival and Growth Test New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CET1Sv1.8,4 Analyzed: 23 Feb-12 15:04 Analysis: Nonparametric-Control vs Treatments Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: 07 Feb-12 12:20 Protocol: EPN82I/R (2002) Diluent: Ending Date: 14 Feb-12 12:40 Species: Pimephales promelas Brine: Duration: 7d Oh Source: In-House Culture Age: <24h Sample ID: Code: ClIent: Test America Sample Date: 06 Feb-12 Material: WWTF Effluent Project: Receive Date: 07 Feb-12 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Angular (Ccrrected) NA C> T NA NA 100 >100 NA I &91% Steel Many-One Rank Sum Test Control vs C-% Test Stat Critical Ties DP P-Value P-Type Decision(a:5%) Lab Water ,6105 Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-Significant Effect ,8333 Asymp Non-Significant Effect Asymp Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAG Limits Overlap Decision Control Resp NL Yes Passes Acceptability Criteria ANOVA Table Source Sum Squares Mean Square DE F Stat P-Value Declsion(e:5%) Between , Non-Significant Effect Error 0, Total Distributional Tests Attribute Test Test Stat Critical P-Value Decision(e:1%) Variances Mod Levene Equality of Variance Equal Variances Variances Levene Equality of Variance , Equal Variances Distribution Shapiro-Wilk W Normahty < Non-normal Distribution 7d Survival Rate Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max Std Err CV% %Effect 0 Lab Water I % 0.0% 6, I % 5.0% % 2.5% % 2.5% % 0.0% % 2.5% Angular (Corrected) Transformed Summary C-% Control Type Count 0 Lab Water Mean % LCL , % IJCL Median Mm Max Std Err ,04074 CV% 0.0% % 5.94% 5.94% 0,0% 5.94% %Effect 0.0% 5.4% 2.89% 2.89% 0.0% 289% CETISIM v Analyst: QA: Page 33 of 53

37 I CETIS Analytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 23 Feb-12 15:05 (p2 of 4) b I New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CETISvI.8.4 Analyzed: 23 Feb-12 15:04 Analysis: Nonparametric-Control vs Treatments Official Results: Yes Yd Survival Rate Detail G-% Control Type Rep 1 Rep 2 Rep 3 Rep 4 0 LabWater I I I I ,9 Angular (Corrected) Transformed Detail C-% Control Type Rep I Rep2 Rep3 Rep4 0 Lab Water , , d Survival Rate Binomials C-% Control Typo Rep I Rep 2 Rep 3 Rep 4 0 Lab Water 10/10 10/10 10/10 10/ID /10 10/10 8/10 10/10 12,5 10/10 10/10 10/10 9/ /10 10/10 10/10 9/ /10 10/10 10/10 10/ /10 10/10 9/10 10/10 Graphics IL Os S 01 Os - p q Q0 Se S S S Os O,IQ 02-4, F I I I F I S OaoIdb I CETIS~ vi Analyst QA: Page 34 of 53

38 CETIS An&ytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 16 Feb-12 13:54 (p1 of 4) b New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CET1SvI.8,4 Analyzed: 16 Feb-12 13:54 Analysis: Nonparametric-Control vs Treatments Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: 07 Feb-12 12:20 Protocol: EPAJ82I/R (2002) Diluent: Ending Date: 14 Feb-12 12:40 Species: Pimephales promeias Brine: Duration: 7d Oh Source: in-house Culture Age: <24h Sample ID: Code: Client: Test America Sample Date: 06 Feb-12 Material: WWTF Effluent Project: Receive Date: 07 Feb-12 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Angular (Corrected) NA C> T NA NA 100 MOO NA % Steel Many-One Rank Sum Test Control vs C-% Test Stat Critical Ties DF P-Value P-Type Declslon(c:5%) Dilution Water Asymp Non-Significant Effect ,8729 Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Contrci Resp NL Yes Beiow Acceptabtuty Criteria ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Declslcn(o:5%) Between Non-Significant Effect Error Tclai Distributional Tests Attribute Test Test Stat Critical P-Value Decision(a:1%) Variances Mod Levene Equahty of Variance 0, Equal Variances Variances Levene EqualIty of Variance Unequal Variances Distribution Shapiro-Wiik W Ncrmahty < Ncn-normai Distribution ld Survival Rate Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max Std Err CV% %Effect 0 Diiuticn Water % 0.0% % % M % -30.0% 25 4 OM % -30.0% % % % -30.0% Angular (Corrected) Transformed Summary C-% Control Type Count Mean 95% LCL 95% UCL Median NUn Max Std Err CV% %Effect Dilution Water % 0.0% % % ,94% % % % 4 1, % % , % % CETISTh vl.&4.24 Page 35 of 53 Analyst: QA:

39 CETIS Analytical Report Report Date: 15 Feb-12 13:54 (p2 of 4) Fathead Minnow 7-d Larval Survival and Growth Test Test Code: Analysis ID; Endpoint: 7d Survival Rate CETIS Version: CE11SvI.8.4 Analyzed; 16 Feb-12 13:54 AnalysIs: Nonparametric-Control vs Treatments Official Results: Yes Td Survival Rate Detail C-% Control Type Rep I Rep 2 Rep 3 Rep 4 0 Dilution Water Angular (Corrected) Transformed Detail C-% Control Type Rep I Rep 2 Rep 3 Rep 4 C Dilution Water , d Survival Rate Binomials C-% Control Type Rep I Rep 2 Rep 3 Rep 4 0 Dilution Water 10/10 0/10 10/lu 10/ /10 10/10 8/10 10/ /10 10/10 10/10 9/ /10 10/10 10/10 9/ /10 10/10 10/10 10/ /10 10/10 9/10 10/10 Graphics b I New England Bioassay 0~9 C.? j ~ L 00 C co 0~ I~ ~ CETISTM vl, Analyst: QA: Page 36 of 53

40 NEW ENGLAND BIOASSAY- WEIGHT DATA FOR FATHEAD MINNOW LARVAL SURVIVAL AND GROWTH TEST Facility Name and Address: West Valley Demonstrotion Project WSNPOOI, Rook Springs Rd West Valley, NY NEB Prrdeot Number Q~ ay-44~4o NEB Test Number Q Test Dates: a I i ~ Weighing Date: ~2/i5 )jp Drying Temperature ( C): 100 Drying Time (bouts): 6 Analyst Initial Weights CA): C~ ~ L~ /, ~ Analyst Final Weights (B): Mv Effluent Concentration Replioate Number Weight of boat (n1g~ Dry weight: foil and larvae (mg~ NEBLab A B Synthetio Controi C q&o, g 3~- ~ 3 D 9&tess q3q4~, A g3q,o7 Receiving Water B ~. 4.c~ Dihient C D A 9zC,,YZ 6.25% B fsi, 71 C D A g&o~lse 115% 8 9z~c C ic~cqs~? 9z71fl D A ~ 923i? 25% B ~H&OI c q:cs,sc ~ 9ccy.o7 9;3,z7 A g (Q,Lj7 50% B gir)!no tu~,1i ~ D A 100% B ~ D QITL~3 gca9y-i- Analyet~_ Page 37 of 53

41 CETIS Analytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 23 Feb-12 15:05 (p3 of 4) b New England Bioassay Analysis ID: Endpoint: Mean Dry Weight-mg CETIS Version: CETISvI.8.4 Analyzed: 23 Feb-12 15:04 Analysis: Parametric-Conb-ol vs Treatments Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: 07 Feb-12 12:20 Protocol: EPN82I/R (2002) Diluent: Ending Date: 14 Feb-12 12:40 Species: Pimephales promelas Brine: Duration: 7d Oh Source: In-House Culture Age: <24h Sample ID: Code: Client: TestAmerica Sample Date: 06 Feb-12 Material: WWTF Effluent Project: Receive Date: 07 Feb-I 2 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Untransformed NA C>T NA NA 100 >100 NA 1 148% Dunnett Multiple Comparison Test Control vs C-% Test Stat Critical MW DF P-Value P-Type Decision(ci:5%) Lab Water CDF Non-Significant Effect ,407 0M CDF Non-Significant Effect , ,9748 CDF Non-Significant Effect CDF Non-Significant Effect CDF Non-Significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(o:5%) Between Non-SIgnificant Effect Error Total Distributional Tests Attribute Test Test Stat Critical P-Value Decislon(a:1%) Variances Bartlett Equality of Variance Equal Vaiiances Distribution Shapiro-WillcW Normality Normal Distribution Mean Dry Weight-mg Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max SW Err CV% %Effect 0 Lab Water , % 0.0% , % 0.87% % -5.57% % -5.28% , ,82% -8.67% % 2,23% Mean Dry Weight-mg Detail Control Type Lab Water Rep I ,524 Rep Rep 3 0, Rep i 84-1 CETISTh vi Analyst: QA: Page 38 of 53

42 CETIS Analytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 23 Feb-12 15:05 (p4 of 4) b I New England Bioassay Analysis ID: Endpoint: Mean Dry Weight-mg CETIS Version: CET1SvI.8.4 Analyzed: 23 Feb-12 15:04 Analysis: Parametric-Control vs Treatments Official Results: Yes Graphics CM 0.5 OOi2~ I. S x 04 OS I It , F &0S 52.5 OS I -to to k I It CETIS vi Analyst QA:. Page 39 of 53

43 CETIS Analytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 16 Feb-12 13:54 (p3 of 4) b New England Bioassay Analysis ID: Endpoint: Mean Dry Weight-mg CETIS Version: CETISv1.8.4 Analyzed: 16 Feb-12 13:54 Analysis: Nonparametric-Control vs Treatments Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: 07 Feb-12 12:20 Protocol: EPN82I/R (2002) Dhluent: Ending Date: 14 Feb-12 12:40 Species: Pimephales promelas Brine: Duration: 7d Oh Source: In-House Culture Age: <24h Sample ID: Code: Client: TestAmerica Sample Date: 06 Feb-12 Material: WWTF Effluent Project: Receive Date: 07 Feb-12 Source: West Valley Demonstration Project (NY000 Sample Age: 36h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Untransformed NA C >T NA NA 100 >100 NA 1 496% Steel Many-One Rank Sum Test Control vs C-% Test Stat Critical Ties DF P-Value P-Type Decislon(a:5%) Dilution Water Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(o:5%) Between , Non-SignIficant Effect Error Total Distributional Tests Attribute Test Test Stat Critical P-Value Deoision(ccl%) Variances Bartlett Equality of Variance ,09 < Unequal Variances Distribution Shapiro-Wilk W Normality < Non-normal Disifibution Mean Dry Weight-mg Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max Std Err CV% %Effect 0 Dilution Water % 0.0% % % % % % % % -30.1% % % Mean Dry Weight-mg DetaIl - 0 6, Control Type Dilution Water Rep I ,586 0,524 Rep 2 0 0, Rep 3 Rep4 0, , I CETIS TM vl Analyst: QA: Page 40 of 53

44 CETIS Analytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 16 Feb-12 13:54 (p4 of 4) b New England Bioassay Analysis ID: EndpoInt: Mean Dry Weight-mg CETIS Version: CETISvI.8.4 Analyzed: 16 Feb-12 13:54 Analysis: Nonparametrlo-Control vs Treatments Official Results: Yes GraphIcs S l C..0.4 Sb L RookIlo i CETIS 4 vi Analyst: QA: Page 41 of 53

45 Final Weight Tare Weight Fish Weight weight/i 0 Mean 1 928, , Page 42 of 53

46 NEB a DATA SKEET FOR ROUTINE CHEMICAL AND PHYSICAL DETERME~ATIONS Paae I of 3 FACILITYNAME & ADDRESS: West Valley De,nonstn,tion PLoject WSNPO0I, Rock Springs Rd West Valley, NY NEB PROJECTNUMBER DS & co TESTOROANISM: Pimephales piomelas DILUTION WATER SOURCE: Receiving Water START DATE: ~ij j ( ~ IT~ j~9iq A24ALYST MV NEBLab REMARKS Synthetic Control Temp., OC Initial 14,2-,Jj4,4 Z~3 a~o 94? z-q~g Q4n D.O.,mg/U Initial $~3 ~,o 12 %1 1-7 ~ ph,sat 7/7 7~ ] y 1 Conductivity, ItS Initial 32) c~u~ 3ttS 3 i ~~5 ~L~ 3 3~)3 &13 Teinp., C Final at~~3 Z~ ~ 2.41) ~to Ef 0 ~f.o Z%,4 D.O~ngIL Final v-~ -1~ 3 ThO (p.s 5,8 ~1c ph, s.u. Final -~ ~ 4j~ -15 ~çq rr.rr 7~,q Conductivity, lis Final 3145 J1~ 354 ~ ~ I ~ Receiving Water REMARKS Diluent Ternp~ C Initial z j,o ~a~ tc zvc?&o ~cp,o 21+0 ~24~ D.O.,mg/L Initial gi/~ g,6 7 ~ 91 a1 ~9 Sn ph,sal Initial q~ 7t1 7-3 t a 7.3 ra ConductivityjiS Initial jqi_ ;c3 3 iq 3 ii v~ av cqj 17 Tem~i, C Final ;Lj,c~ ~~IIO ~ ~44 24~O ~Lj:o 7 4~~ ao.,mg, L Final Z7 ~ 7ç 14 (p1 r73 ]~1 ph, au. Final 7t0 ~fle 7Lo r),3 ~,g T(o ii Conductivity, ~ Final ZZ3 2-I ~o ig~i. 2L~3 ~~t5 142_ Z 4 / 6.25% REMARKS Temp., C Initial 24L0 &40 ZQ~t 2u1~O ~ 24.0 >c~s!4 D.O.,tngiL Initial 1O.C~ g,; q,lj 9-7 %~C~ e~7 8.3 ph,s.u. Initial -j,l~ ~ ta 7~ 1 14 ConductivitypS Initial ~ 274 Z-tc0 ~ 211 c7 ~7 Teinp., C Final &t4~ z~i~c 2~j1~ ao 2LI~0 ~44 zci,z, D.O.,nig/L Final )~7 %7~ 7) r70 ~9 ry (,p 4,q ph,sat Final -j~ 7{~, 7~ I 7Q. 7~7 7U> 71C ConductivityjiS Final ~)O 3 i j ~2S~ 312-3~1.+ ~ z7 Ic 12,5% REMARKS Temp,, C Initial.~ ~ z~.c z~&o a~e 29~O ~ D.O~mWL Initial ift q,i. Q M 9.-a ph,s.tt Initial 714 7~Jf -~ Conductivity, its initial 37 ~ q~ 3 g~ 3~3 ~c3s3 373 Tenip~C Final A~I4 ~*\ 21F~O ~ ~610 DD.,mgJL Final 7,~ tn (p~ (o1 ~TO ~1LI 4M pl-i s.u. Final 7Cc ~ ~7$ 7(0 7,c~- Conductivity, ~ Final 34 Lj 5~~-.5q7 L~2- i~t I s ~) q ç m- Clv(~LQd /ø c.t Page 43 of 53

47 NEB s DATA SHEET FOR ROUTINE CHEMICAL AND PHYSICAL DETERMINATIONS Page~ of 2 FACILITY NAME & ADDRESS: West Valley DeinonstrationProject WSNPOOI, Rock Spr ngs Rd West Valley, NY NEB PROJF.CTNtjMBER: O&00LN94 O. DO TEST ORGANISM: Pimephales promelas DIUTION WATER SOURCE: Receiving Water STARTDATE: ~9I7 11,9 T11~~E; jaarj 25% REMARKS Temp.. C Initial zlc~o ~ 21k0 iq~ at+o ale, o 0~4~j D.O.,tngfL Initial 9~ 3,3 ~ Ct3 jp I S~3 ph,sat Initial 74 79,~?L1 ~4. Conductivit~ ~ Initial 6 Ci ~c ~i-f q44 ~5V& 5 ~\ 53 ~ TemP. ~ OC Final ~ V4 V jl(,3 aq~.3 ZLf, p ~ LHn ~vi4q Dfl,mg/L Final 7Z 7,! 1L~ t7~3 ~p (j2s (~C7 ph,s.u, Final -~ ~ 7(,~ ~75 ~3 71o 7C Conductivity, ItS Final 5 ~ C ) ~ ~ 50% REMARKS Teinp~ C Initial 1(-i~O ~ z i~c~ zt -o a tc zcj~4 ç~q,(~ D.O.,nig/L Initial 91i q,3 t(9e %3 3~3 s8 ph,sal Initial ~ 7,7 7-3 ~ ~7 Conductivit, ItS Initial ~ g~ 7 ~ 84 3 ~St &~s~ ~A~.L1- Temp., C Final?~cQ 214M 2gg alko z~p eh,, ic I D.O.,mgIL Final 7,5 7 j (~Q ~ ) -~ ~ Q~ ph,sa~ Final 77 7~ ye~ ~ 37) j7c53 t7fl Conductivity,pS Final ~ $-~f C\QQ ~ i 100% REMARKS Temp., C Initial 1(4 to ~Jf p -zc~c? [-O ~cj-,o z~t ~4. I flo~ing/l Initial q, I 7!j4 9,1 i/~b 9-7 ~]-~5 ph,s~t Initial 7,-7 7~ 7~7 -:94 ~ ~7 ~] Conductivity,gS Initial ~çq~ ~ 1Gb PW6 jqs3 ~ q~ I~-14 5 Temp., C Final ~ ~- z~13 z4.4- a ~-1o 2 -f~ tj O24(~ Z~n g D,O.,ing/L Final ~ 7~ (or (~$ ~ 3 To ~? ph,s.u, Final vi 7<? <?O ~ ~1 a~.o 7,q Conductivfty,~tS Final 15 ) ((QZ5 Hoog i~si 151t.+ IH 7~ / 17~f Temp., C DO., mg/l ph, s.u. Initial Initial Initial REMARKS Conductivity, ItS Initial Temp., C DO., nig/l ph, s.u. Final Final Final Conductivity, ps Final Page 44 of 53

48 INITIAL CHEMISTRY INFORMATION CLIENT: JOB # TEST #: Test America West Valley O5. OOQ4&LFo.or~ C dithialdno. (~ Y7Z Yi P. yrornelasldno. Ia-&7~f h SAMPLE TYPE: Effluent River Effluent River Effluent River #1 Water #1 #2 Water #2 #3 Water #3 RECEIPT DATE ~ / 7//~~Z ~2/ 7/i~2 Z-/i0I4 2 i/o/i COC# ~?~1 q$~ 02 ~1W? C12~/f~~ PH(SU) 7,9 7,3 Temperature( C) 1)t,cQ3 5 70) 2? Dissolved Oxygen (rng/l) ~3 Specific Conductivity (pathos/cm) /4g~ /3 / 205 Salinity(ppt) ~ f ~ I TRC-DPD (rng/l) TRC - Amperornetric )4 O~d23 N? 0,cV ~p N o,o15 N P~ (ing/l) Hardness (mg/las CaCO3) too 70 Alkalinity (mg/l as CaCO3) /70 Color J~M I~~7ik-?jw~&_:) clknzu~ yc/1v ~. Clarity ctzxr ejeo-( (4 r INITIALS ~- i3r 3*- 7W NOTE: NA = NOT APPÜCABLE~TY A- Data Reviewed By: cail~ Date Reviewed: Page 45 of 53

49 832 AppliedLinear Statistical Model ~ ~J~\J~j~ TABLE A 14 GL~e~ it: Table of Random Perm UnouiS -fea-(- (u~ ~ ti o it 9 4 ii It Is to 11 t I-S IS H- 7 ~S ~ ii It II I It ~ i2~g g Ii ~ I Ii i~ ii it H ii S ~ ~ Ii I 20 8 H t: U IS II 3 II Jo IS II II J2 S It I It I IS IC /1 1V ;.; I it ( II to I it Ii It I Ii ~ it S I ! II II S It It t !5 Source: Rcp:in~ed, 2W p~rm2~sion, ::c-m Wi2Ii~m 0. CocIi~an und G:rt~4~ ~. Co-S. Ex;erinwi:n: Nrv York:Joh~\s2I~y &SOrSIn:., 1c571, p Page 46 ofss

50 t36cwi ~LL EFFLUENT RECEIVING WATER Sampler: C. r ~ CSCL. Sampler: - Title: Title: F7-r.~j inr~n&~taij~.~ Facility: West Valley Facility: West Valley Sampling Method: X Composite Sampling Method: X Grab Sample ID: Outfall 001 Sample ID: Franks Creek Start Date: 2-r~ i2. Time: /StO Date Collected: ZGci 7 End Date: 2Q C Time: /.s~bo Time Colkcted: or1 La Sampling Method: Grab (for ph and TRC only Date Collected: Time Collected: Sample Type: Preoblorinated - Dechlorinated Effluent Sampling Location and Procedures: L-3 cj~c (-$c co i~ a~,(~4 ~I~ ~pa-p Ffo~ Ce~çc~4~e O tlf(fn3 Receiving Water Sampling Location and Procedures:._r~-~yc~~ c.-~-ejc (~n\.frc~-i5 a~wp1j v ~cs Jv~.-t Czrc4-, Requested Analysis: X Chronic and modified acute p Method of Shipment: Relinquished By: Date: Time: Received By: Date: Time: Relinquished By: Date: Time: Received By: Date: Time: Relinquished By: Date: Time: Received Bc~~øL Tpp Date: 4 7//~ Time: 08 Temperature of Effluent Upon Receipt at Lab: Temperature of Receiving Water Upon Receipt at Lab:0?.OfQ f C Effluent COO# /L/ q~ Receiving Water COC# C2~2 /~ ~S IF THIS COOLER IS MISPLACED OR THE LABEL IS LOST, PLEASE SHIP TO: KIM WILLS, NEW ENGLAND BIOASSAY, 77 BATSON DRIVE, MANCHESTER CT Page 47 of 53

51 CH2M Hill B&W West Valley LLC (CHBWV) Rock Springs Rd. West Valley, NY CHAIN-OF-CUSTODY I REQUEST-FOR-ANALYSIS I PACKING SKEET Sample Type: SPDES Electronic Disk - Yes External Lab Destination Purchase Order Number Charge Number I Release Number Report Format Level Priority TestAmerica ~ CH I WV03.1NML0L0L Business Days ~sto~an Signature AZ ga1a)~ e~ C.O-C ReportDatato: ChetWro~~ (71~942416O Notes WNSP0OI /06/ Cool Notes: Samples shipped under contract with Test America 0, U, 0) C, Cu a- Signature Rel: Date/Time Signature Rec: Date/Ti~~~~ Signature Rel: Date114a_~ Signature Rec: Date/Time A~~k/e~r z/4/ze / SO Signature Reb Date/Time ~,Q/97~(~Q~C~ Signature Ree: Date/Time Signature Rel; Date/Time Signature Rec: Date/Time Signature Rel: Date/Time Sample Receipt at Lab: Cool YES NO Tenip: C Signature Rec: Date/Time Custody Tape Intact: YES NC) Page 1 of I

52 NEW LJOASc~AJN-OF-cTJSTODy j EFFLUENT RECEiVING WATER Sampler: Sampler: ~ ~ ~t3cl~ Title:,r OAø1t~,4 i ~i~4~ Facility: West Valley Facility: West Valley Title: fe-ti 3 iov ~ V$10t-th k. sci,~i,d1zcj4 Sampling Method: X Composite Sampling Method: Sample rn Start Date: p 9 c 2_~ Outfall 001 Time: /c,to Sample ID: Date Collected: End Date: L jvl_ Time: /cj, to Time Collected: X Grab Franks Creek Sampling Methoch Grab (for ph and TRC only ) Date Collected; Time Collected: Sample Tyne: it Unchlorinated Chlorinated Effluent Sampling Location and Procedures: L- S ~(spocil~n o~,l~j U~ C-2 f1o~a Receiving Water Sampling Location and.procedures: ~flns 4 c ~c~4pt ( n, ~r-&axts Cr~q~ ~C (cmi FtC \~ ~ S-iNV-2 I ~ Requested Analysis: X Chronic and modified acute ~ ~. ~ Method of Shipment: Relinquisbed By: / / /~/ Date: Received By: Date: z/io/,z Relinquished By: Received By: Relinquished By: Temperature of Effluent Upon Receipt at Lab: O~P(~C Date: Date: Received By: Date: - Time: Effluent COC# Date: ~: Time: Time: 0c1 00 Time: Time: Time: Temperature of Receiving Water Upon Receipt at LabL~)2I C C3 Z (cc Lj Receiving Water COC# C 32 jsvc IF TifiS COOLER IS MJSPLACED OR THE LABEL IS LOST, PLEASE SHIP TO: KIM WILLS, NEW ENGLANI) BIOASSAY, 77 BATSON DRIVE, MANCHESTER CT Page 49 of 53

53 CH2M Hill B&W West Valley LLC (CHEW. ) Rock Springs Rd. West Valley, NY CHAiN-OF-CUSTODY I REQUEST-FOR-ANALYSIS I PACKING SHEET Sample Type: SPDES Eleefronic Disk- Yes External Lab Destination Purchase Order Number Charge Number Release Number Report Format Level Priority TestAnierica CH WVO3.]N.O1.Ol.Ol Business flays Custodian Signature P fr,-_- C-O-C Reviewed By: I%1/t4a_4 -.~4L Z - ~ -( t- Report Data to: Chet Wrotniak (7i~ ~Jotes: WNSPOO J 02/09/ I Cool WET Notes Samples are being sent via contract with Test America Signature Rel: Date/Time W6~h~fl Signature Rec: Date/Time Signature Rel: Date/Time,1 ~ IA 41 I /2 ~ / Signature Rel: Rec: DatelTime Date/Time Signature Rel: Date/Time Signature Rec: Date/Time Signature Ret Date/Time Signature Rel: Date/Time Sample Receipt at Lab: Cool YES NO Temp: C Signature Rec: Date/Time Custody Tape Intact YES NO Page 1 ofl

54 ORIGIN ID DKKA (716) KEN ~tnecki TESTAMERICA 10 HA2ELN000 OR - AMHERST, NY UNITED STATES Us T0SAIVIPLE RECEIVING NEW ENGLAND BIOASSAY~ 77 BATSON DRIVE MANCHESTER CT (860) REF: WEI TESTING DEPT: SAMPLE CONTROL IN P~ IIIIIIIUIIIIII 1111 (1111tH IIIIIIIIIIIIIIII I III! I4~ ~~y*i Fi~ ~ F hj~. SHIP DATE: 09FEB12 ACTIJGT: 66.0 LB han CAD:?35603/CAFE25OS BILL SENDER ~ o~1 ~j ILl a (ii ii~irum ~i ~ ~fll IiIII Express ~ \ ~ ~ Fed Lx I k~ ~ i( r~ix FRI 1OFEB A2 ~ PRIORITY OVERNIGHT ZB QCWA I~llt~ ~ ~ II11~~ II I~UI~~ li U iii CT-US 801 ORIGIN ID: OKKA çfls) KEN KINECKI TESTAMERICA 10 NAZELM000 DR I SI-lIP DATE OSFEBIZ ACTWGT. eo.0 LB MAN CAO~ t)CAFE2SOS AMHERST. NY BILL SENDER UNITED STATES US TO SAMPLE RECEIVING NEW ENGLAND BIOASSAY 77 BATSON DRIVE MANCHESTER (8~) 643~9660 OEPL SAMPU CONTROL CT REF: WET TESTING fr~~44~5 O ZB ocw1~ PRIORITI OVERNIGHT g~o42 CT-US 801 C C 0 04 Ca CC 0 a a.

55 Sodium Chloride Reference Toxicant Data Ceriodaphnia dubia Chronic Toxicity MAR FEB ,901 C 1 ~ C C C ~ 0.30 oioj I I I I I I I I I C -010 r 5 ; c~ ~ - J C Test Date 1C25 Reproducllon Mean 1C25 ± 2 SD Test ID Date IC25 Mean IC25 STD -2STD +2STD cv % /1/ /1/ /17/ /21/ /1/ /2/ /1/ /4/ /2/ /1/ /5/ /3/ , /1/ /1/ /2/ /1/ /1/ /1/ /1/ /3/ /8/ /1/ /4/ /15/ Page 52 of 53

56 Reference Toxicant Data 7-day Chronic Toxicity Test P. promelas Growth Data 1.50 FEB FEB 2012 x x x -J ? < x >< )< (5 (U --- Z o o N r ~- t- o o ~ N ~N Z 0 Ef) ~.. 0) r Test Date X 025 Mean 025 ±2 SD Test ID Date IC25 Mean IC25 STD -2STD +2STD CV % /1/ /1/ , /1/ /4/ /21/ /1/ /1/ , /4/ /2/ /1/ /5/ /3/ /1/ /1/ /2/ /1/ /1/ /1/ /1/ , /3/ /8/ /1/ /4/ /1/ Page 53 of 53

57 WR:2012:0030 Attachment 2

58 Th ma THE LEADER IN ENVIRONMENTAL TESTING ANALYTICAL REPORT TestAmerica Laboratories, Inc. TestAmerica Buffalo 10 Hazelwood Drive Amherst, NY Tel: (716) TestAmerica Job ID: TestAmerica Sample Delivery Group: 120/1 23 Client Project/Site: West Valley SPOES CH2M Hill B&W West Valley (CHBWV) MS-ELAB West Valley, New York Attn: Chester Wrotniak Authorized for release by: 4/26/ :58:00 PM John Schove Project Manager I john.schove~testamericainc.com LINKS Review your project results through ToldAccess Have a Question? Ask 7k a Visit us at I II C Ex e rt The test results in this report meet all 2003 NELAC and 2009 TNI requirements for accredited parameters, exceptions are noted in this report. This report may not be reproduced except in full, and with written approval from the laboratory. For questions please contact the Project Manager This report has been electronically signed and authorized by the signatory. Electronic signature is intended to be the legally binding equivalent of a traditionally handwritten signature. Results relate only to the items tested and the sample(s) as received by the laboratory. Page 1 of 50

59 Case Narrative Client: CH2M Hill B&W West Valle (CBWV) TestAmerica Job ID: ProjectiSite: West Valley SPDES SDG: 120/I 23 Job ID: Laboratory: TestAmerica Buffalo Narrative Job Narrative Comments This report has been revised to change the name of the receiving water collection location from Franks Creek to Erdman Brook. This report has been revised to correct typographical errors. Chronic Toxicity was performed by New England Bioassay. Final results are contained under this cover. Receipt No analytical or quality issues were noted. Page 2 of 50 TestAmerica Buffalo

60 CRROMC WHOLE EFFLUENT TOXICITY TEST REPORT West Valley Demonstration Project West Valley, New York Receiving Water: Erdman Brook NPDES Permit: NY Test Start Date: March 10, 2012 Test Period: March 2012 Report Prepared by: New England Bioassay A division of GZA GeoEnvironmental, Inc. 77 Batson Drive Manchester CT NEll Project Number: Report Date: March 26, 2012 Report Submitted to: Test America, Buffalo 10 Hazelwood Drive Antherst, NY Sample ID: /02049 Please contact the Lab Supervisor,Kim Wills, at (860) or 1dmber1y.wills~gza,com if you have any questions concerning these results. Page 3 of 50

61 Whole Effluent Toxicity Testing Report Instruction Form Client Name/Project: Test America! West Valley 007 Test Date: 3110/12 Sample ID: /02049 Your results were as follows: )~ ass o Fail Please proceed according to the instructions in your permit. o Invalid Retesting is still required. Retest report will be sent at a later date under separate cover. o Original Test invalid Valid retest performed. Both test and retest results are attached. o Retesting will be or has been performed according to the Case 1 Protocols outlined in the attached copy of EPA-New England s species-specific, self-implementing policy for alternate dilution water. o Ihis is your case of dilution water toxicity. Please proceed according to the Case 2 Protocols outlined in the attached copy of EPA-New England s species-specific, self-implementing policy for alternate dilution water. The alternate dilution water you select for future tests for this species should be described as follows: synthetic laboratory water made up according to EPA s toxicity test protocols, by adding specified amounts of salts into deionized water in order to match the hardness of our receiving water. Writing this letter should help you to avoid rete,sts in the ffiture. o Available information is insufficient to determine whether this test passed or failed. Please compare results to your permit limits. Please submit a current copy of your permit to the NEB Lab so that we can detennine the status of future tests results and help ensure your compliance with pennit requirements. Please complete the items on this list before reporting these results according to the instructions in the Monitoring and Reporting Section of your permit. Please complete, sign and date the upper portion of the Whole Effluent Toxicity Test Report Certification page which is the page directly following this page. Fill in the Sample Type and Sample Method (upper right) and the Permit Limits (lower left) on the New England Bioassay-EPA Toxicity Test Summary Sheet(s) if they are incomplete. Fill in any missing information on the NB Chain-of-Custody documents. This includes ensuring that the following information is recorded: Sampler s name and title, Facility name and address, Sample collection methods, Sample collection start and end dates and times, Types of sample, Chlorination status of samples upon shipment to NEB, Site description and Sample collection procedures. Monitoring results should be summarized on your monthly Discharge Monitoring Report Form. Signed and dated originals of this report must be submitted to the State (and Federal) Agencies specified in the Monitoring and Reporting section of your permit Questions? Please contact the Lab Supervisor, Kim Wifis, at (860) or Mmberlv.willsa~gza.com. Page 4 of 50

62 WHOLE EFFLUENT TOXICITY TEST REPORT CERTIFICATION (Pemiittee) I certify under penalty of law that this document and all Afl ACHMENTS were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accwate, and complete. t am aware that there are significant penalties for submitting false information, including thc possibility of fine and iniprisomnent for knowing violations. Executed on ~ 1 2 [Date] ~ uth~r1 zed Signature] Bryan C. Bower, Director [Print or Type Name and Title] West Valley Demonstration Project [Print or Type the Permittee s Name] NY [Print or Type the NPDES Pem~iit No.] Since the WET test and report check is complicated, the New England Bioassay Aquatic Toxicity Laboratory has certified the validity of the WET test data in the section below. Please note that this does not relieve the pernñttee from its responsibility to sign and certify the report under 40 C.F.R (k). WHOLE EFFLUENT TOXICITY TEST REPORT CERTIFICATION (Bioassay Laboratory) I certify under penalty of law that this document and all ATTACHIVIENTS were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Executedon ~m [Date] [Autho ized Signature] Kim Wills. Laboratory Supervisor [Print or Type Name and Title] New England Bioassay [Print or Type Name of Bioassay Laboratory] 24. Telephone Contacts If you have questions, please contact Joy Hilton, Water Technical Unit, at (617) or David McDonald, Ecosystem Assessment Unit, at (617) Page 5 of 50

63 AQUATIC TOXICITY TEST SUMMARY Client: Sample ID: NPDES Number: West Valley Demonstration Project Outfall 007 and Erdman Brook NY NEB Prolect Number: NEB Test Numbers: A&B Sample Dates: 3/ /12-13/12 (Effluent) ,3 13/12 (Receiving Water) Test Dates: 3/10-17/12 Test Type: Test DuratIon: Test species: Dilution Water Source: Test Method: Concentrations Tested: Modified Chronic Static Renewal Freshwater Test 6 days (Ceriodaphnia) & 7 days (Pimephales) Ceriodaphnia dubia and Pimephales proinelas Erdman Broook U.S. Environmental Protection Agency (EPA) Document EPA-82 1-R , Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms, Fourth Edition 0%, 6.25%, 12.5%, 25%, 50% and 100% effluent Acute Results: Ceriodaphnia dubia 48-hour LC5O = >100% Pimephales promelas 48-hour LC5O = >100% Ceriodaphnia dubia Wa = 0.3 Pimephalespromelas Wa Chronic Results: Ceriodaphnia dubia C-NOEC Pimephalespromelas C-NOEC Ceriodaphnia dubia TUc Pimephalespronielas TUc = ~Q Mean Control Survival: Ceriodaphnia dithia / (laboratory control) and ~ (dilution water) Pimephalespromelcis.- 100% flaboratory control) and 2~4 (dilution water) Reference Toxicant Data: Date: 3/1/12 (Ceriodayhnia dubia and Pirneyhales yromelas) Toxlcant: Sodium chloride Source: New England Bioassay jq~: Ceriodaphnia dubia 1.26 a/l Pimephalespromelas all Page 6 of 50

64 New England Bioassay a division of GZA EPA Toxicity Test Summary Sheet Facility Name: West Valley Demonstration Project Test Start Date: 3/10/12 NPDES Permit Number: NY Pipe Number: Outfall 007 Test Tyue Test Species Sample Tvve Sample Method Acute Fathead Minnow Prechiorinated Grab Chronic X Ceriodaphnia Dechlorinated X Composite ~ Modified Daphnia Pulex Chlorine Spiked in Lab Flowthru (chronic reporting Mysid Shrimp Chlorinated on site Other acute values) Sheepshead X Unchiorinated 24hr screening Menidia Sea Urchin _Champia TRC: mg/l Selenastrum Other Dilution Water X receiving water collected at a point upstream of or away from the discharge, free from toxicity or other sources of contamination; (Receiving water name: Erdman Brook) alternate surface water of known quality and a hardness, etc. to generally reflect the characteristics of the receiving water; (Surface water name: synthetic water prepared using either Millipore Mill-Q or equivalent deionized water and reagent grade chemicals; or deionized water combined with mineral water; or artificial sea salts mixed with deionized water; deionized water and hypcrsaline brine; or other Effluent sampling date (s): 3/8-9/12 3/12-13/12 Effluent concentrations tested (in%): 0 ~ * Permit limit concentration: TUa < 0.3. TI.Jc < 1.0 Was effluent salinity adjusted? 112 If yes, to what value? ~JL~ ppt With sea salts? N/A Ilypersaline brine solution? N/A Actual effluent concentrations tested after salinity adjustment (%): I ~ Reference Toxicant test date: 3 1/12 Test Acceptability Criteria Mean Control Survival: 100% Mean Control Reproduction: 30.4 young/female Mean Diluent Survival: 90% Mean Diluent Reproduction:27.2 young/female Limits Results LC5O NA LC5O >100% Upper Value Lower Value 100% Data Analysis Method Used Graphical TIJa 0.3 TUa 0.3 A-NOEC N/A A-NOEC 100% C-NOEC N/A C-NOEC 100% LOEC >100% TUc 1.0 TUc 1.0 1C25 N/A 1C25 >100% 1C50 N/A 1C50 Page 7 of 50

65 New England Bioassay a division of GZA EPA Toxicity Test Summary Sheet Facility Name: West Valley Demonstration Project Test Start Date: 3/10/12 NPDES Permit Number: NY Pipe Number: Outfall 007 Test Type Test Species Sample Type ~mple Method Acute X Fathead Minnow Prechlorinated Grab Chronic Ccriodaphnia Dechlorinated K Composite ~ Modified Daphnia Pulex Chlorine Spiked in Lab Flowthru (chronic reporting Mysid Shrimp Chlorinated on site Other acute values) Sheepshead ~ Unchiorinated 24hr screening Menidia Sea Urchin _Champia TRC: mgll Selenastrum Other Dilution Water K receiving water collected at a point upstream of or away from the discharge, free from toxicity or other sources of contamination; (Receiving water name: ErdmanBrook) alternate surface water of known quality and a hardness, etc. to generally reflect the characteristics of the r~eiving water; (Surface water name: s)mthetic water prepared using either Millipore Mil-Q or equivalent deionized water and reagent grade chemicals; or deionized water combined with mineral water; or artificial sca salts mixed with deionized water; deionized water and hypersaline brine; or other Effluent sampling date (s): 3/8-9/12 3/12-13/12 Effluent concentrations tested (in%): * Permit limit concentration: TUa < 0.3. TUc < 1.0 Was offluent salinity adjusted? No If yes, to what value? WA ppt With sea salts? N/A Hypersaline brine solution? N/A Actual effluent concentrations tested after salinity adjustment (%): Reference Toxicant test date: 3/1/1 2 Test Acceptability Criteria Mean Control Survival: 100% Mean Control Weight: mg Mean Diluent Survival: 95% Mean Diluent Weight: nig Limits Results LC5O N/A LC5O >100% Upper Value +co Lower Value 100% Data Analysis Method Used Graphical TUa 0.3 TUa 0.3 A-NOEC N/A A-NOEC 100% C-NOEC N/A C-NOEC 100% LOEC >100% TUe 1.0 TUc 1.0 1C25 N/A 1C25 >100% 1C25 1C50 >100% Page 8 of 50

66 CERJODAPHNIA DUBIA AOUATTC TOXICITY TEST REPORT Test Reference Manual: EPA 821 -R , Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms, Fourth Edition Test Method: Cerioclaphnicz dtthia Survival and Reproduction Test Test Type: Modified Chronic Static Renewal Freshwater Test Temperature: 25 * 1 C Light Quality: Photoperiod: Test Chamber Size: Test Solution Volume: Ambient Laboratory Illumination 16 hours light, 8 hours dark 30 ml Minimum 15 ml Renewal of Test Solutions: Daily, using most rcccntly collected sample Age of Test Organisms: Less than 24 hours Number of Neonates Per Test Chamber: 1 Number of Replicate Test Chambers Per Treatment: 10 Number of Neonates Per Test Concentration; 10 Feeding Regime: chamber daily. Aeration: Dilution Water: Fed 0.1 ml each of YCT and algal suspension per exposure None Erdman Brook Alternate Control Water: NUB Moderately Hard Water (hardness mg/i,) Effluent Concentrations: 0%, 6.25%, 12.5%, 25%, 50% and 100% effluent Test Duration: Until 60% of control females have three broods - 7 days End Points: Survival and reproduction. Test Acceptability: Control Survival: 80% Yes X No Control Reproduction: Avcragc? 15/control female Yes X No Sampling Requirements: NYSDEC has approved West Valley Demonstration Project to use only two sets of samples for their chronic testing due to the batch nature of their discharge, Sample Volume Required: Minimum 2 liters/day Test Organism Source: NEB Page 9 of 50

67 Test Acceptability Criteria:Mean Control Survival = 100% Mean Dilution Water Control Survival = 90% Mean Control Reproduction ~Q~4/surviving female Mean Dilution Water Control Reproduction =27,~/surviving female Test Results: Limits Results Status 48-hour LC5O >100% Upper Value + ~ Lower Value 100% Data Analysis Method Used Graphical A- 100% TUa hour EC5O > 100% Survival C-NOEC 100% Reproduction C-NOEC 100% Reportable C-NOEC 100% TUc LOEC >100% MAWC >100% 1C25 > 100% Reference Toxicant Data: Date: 3/1/12 Toxicant; Sodium chloride Dilution Water: NEB Lab Synthetic Soft Water Source: New England Bioassay ICE: 1.26WL In Acceptable Ran2e: Yes X No Dechlorination Procedures: ~h1orine is measured using 4500 CL-S DPD Colorimetric Method. X Dechlorination was not required - Sample was dechlorinated by adding sodium thiosulfate to the sample prior to test initiation. Since dechlorination of the effluent was necessary, a thiosulfate control of diluent water spiked with sodium thiosulfate was also included in the test series. Chlorine was mg/l in a dechlorinated sample. - Chlorine Measurement was elevated due to interference. Chlorine was mgt~ in a filtered sample. - Total Residual Chlorine was re-measured following aeration, and was found to be mg/l. Page 10 of 50

68 PIIYIEPHALESP1?OMELAS AQUATIC TOXICITY TEST REPORT Test Reference Manual: EPA 821 -R , Short-Term Methods for Estimating the Chronic Toxicity ofeffluents and Receiving Water to Freshwater Organisms, Fourth Edition Test Method: Fi;nephalesprornelas Larval Survival and Growth Test Test Type: Modified Chronic Static Renewal Freshwater Test Temperature: 25 ± 1 C Light quality: Photoperiod: Test Chamber Size: Test Solution Volume: Ambient Laboratory Illumination 16 hours light, 8 hours dark 600 nil, Minimum 200 nil Renewal of Test Solutions: Daily, using most recently collected sample Age of Test Organisms: Less than 24 hours Number of Larvae Per Test Chamber: 10 Number of Replicate Test Chambers Per Concentration: 4 Number of Larvae Per Test Concentration: 40 Feeding Regime: Fed 0.1 nil newly hatched brine shrimp nauplii twice daily. Cleaning: Siphoned daily, immediately before test solution renewal. Aeration: DO concentration fell below 4.0 mg/l Yes No A Aerated at <100 bubbles/minute Yes No X Dilution Water: Erdman Brook Alternate Control Water: NEB Moderately Hard Water (hardness mgfl) Effluent Concentrations: Test Duration: End Points: 0%, 6.25%, 12.5%, 25%, 50% and 100% effluent 7 days Survival and growth. Test Acceptability: Control Survival:? 80% Yes X No Control Average Dry Weight 0.25 lug Yes X No Sampling Requirements: NYSDEC has approved West Valley Demonstration Project to use only two sets of samples for their chronic testing due to the batch nature of their discharge. Sample Volume Required: Minimum 2.5 liters/day Page Ii of 50

69 Test Organism Source: NEB Test Acceptability Criteria:Mean Control Survival = l00% Mean Dilution Water Control Survival = 95% Mean Control Weight = rng Mean Dilution Water Control Weight = mg Test Results: Limits Results Status 48-hour LC5O >100% Upper Value LowerValue 100% Data Analysis Method Used Graphical A-NOEC 100% TUa hour EC5O >100% Survival C-NOEC 100% Growth C-NOEC 100% Reportable C-NOEC 100% TUe LOEC >100% MAWC >100% 1C25 >100% Reference Toxicant Data: Date: 3/1/12 Toxicant: Sodium chloride Dilution Water: NEB Lab Synthetic Sofi Water Source: New England Bioassay 1.21 gil In Acceptable Range: Yes X No Dechlorination Procedures: chlorine is measured using 4500 CL-G DPD Colorinietric Method. ~ Dechlorination was not required - Sample was dechlorinated by adding sodium thiosulfate to the sample prior to test initiation. Since dechlorination of the effluent was necessary, a thiosulfate control of diluent water spiked with sodium thiosulfate was also included in the test series. Chlorine was mgil in a dechlorinated sample. - Chlorine Measurement was elevated due to interference. Chlorine was mgil in a filtered sample. - Total Residual Chlorine was re-measured following aeration, and was found to be mg/l. Page 12 of 50

70 NEW ENGLAND BIOASSAY TOXICITY DATA FORM CHRONIC COVER SHEET CLIENT: ADDRESS: CONTACT: Test America. Inc. 10 Hazeiwood Drive Amherst, NY West Valley C. dubiatestidno: i~ 1 ~3R Rprornelas TEST ID NO :t ) _4LL3 i-7 COCNO: CiD~ PROJECT NO: D S, Uc 44at1U.OO SAMPLE TYPE: DThUTION WATER SOURCE: Receiving Water INVERTEBRATES TEST SET UP (TECH NIT.): TEST SPECIES: Cerloda ha/a thibia LOT #: 42? it ~1D ) AGE: <24 I-fours TEST SOLUTION VOLUME: 15 ml NO. ORGANISMS PER TEST CHAMBER: I NO. ORGANISMS PER CONCENTRATION: 10 STARTDATE: ENDDATE: 3iioliz_ ~3/L(,//?. AT IDZS hours AT Ct? 1~5 hours VERTEBRATES TEST SET UP (TECH INIt): TEST SPECIES: Pimeyhalçs proinelas SOURCE/NEB LOT#: C~4(2(ELt4~ OS3 ~ AGE: <24 Hours TEST SOLUTION VOLUME: 400 rnl NO. ORGANISMS PERTEST CHAMBER: 10 NO. ORGANISMS PER CONCENTRATION: 40 START DATE: a ho(il AT ICO hours ENDDATE: ~jl9 ~ a AT~ hours Laboratory Control Water Sample Collection Time Test Batch Hardness Day Number mg/i CaCO3..2_ e~oo~..~.fl: ~- I Alkalinity mg/ I CaCO3 CC (-p5. Effluent# I from:.3 I?/Y) at p gc~ç tn: S(q/I7 ~ at Diluent# I b ( 3/ iq atj~yc Effluent #2 from:2ft It? at to: 31f3/IY. at toto Diluent#2 to: ~/i51 17 at ~~~,, Effluent #3 from: (V $~ at hj A to:~jq Pi at Diluent#3 to: N Dr at Results of Ceriodaphnja dub/a Chronic Test % Coni Limit 48HourL~30:>~~~ 4 do 7DayLC50: Survival NOEC: 00 Survival LOEC: >1 ~ ~ cc Reproduction NOEC: Reproduction LOEC: Reproduction 1C25: > [ bd[~ )~ VD 10 NOEC: NO-OBSERVED.EFFECT.CONCENTRATION Results of Plinephales promelas Chronic lest 95% Conf, Limit 48 Hour LC55: >1o~h ~ 7DayLC50: 7 ~ Survival NOEC: }bd 1 ~, Survival LOEC: t >IOb1~ GrowIhNOEC: GiowtliwEC: >~c 1O GrowthIC2S: LOEC: LOWEST.OBSERVED.BFFECT.CONCENTRATION ANIMAL CONDITION/BEHAVIOR: COMMENTS: U I tit,i dqlhlrs Sjp4tk.J &--6 REVIEWED BY: I DATE: Page 13of50

71 NEW ENGLAND BIOASSAY - CHRONIC TOXIC1TY TEST BROOD DATA SHEET Page 1 of 2 FACILITYNAME & ADDRESS: Westvalley Dernonstmtion Prnjeot WSNPOO7, Rook Spnngs Rd West Valley, NY NEB PROJECrNUMBER: o6~ cow f~ q 0 DC) NEB TESTNTJMBER: C) C) c~ COC#:0~ -. TEST ORGANISM: Ceriodapisnia dubia AGE: <24 bouts LOT #: C. 41 ~ ~2riW as STARTDATE: 2 ~ IT~ ~)3 ~ HDATE: ~ T~E: Effluent Day Source 1: Source 2: Total Number Analyst - Analyst - concentration Number Live Live Transfer Counts Young Adults Replicate A B C U E F C HIS 0~ 1 ~T I 4 T ~ 4 4 ~ ~V T (a m&1 ~ (W -~ ~ SII NEBLOb 3~ 5~ L4~ Ot mel Synthetic 4 I JJJ_jJ cxn to f~~d 1311 Control ~ Jj~ 7 ~7 ic)_ JI J~ ~ 4-i- 3 to ~ M 6 ~ij~d ~ fln.lllc...ij{_±c ~Lfl uc jco j-o ii To2flfl~ to Ifl~- 4 1 ~,o S 2 4 T T T -~ ~ -~ -~ to It~!- -r~*~~ Receiving 3 ~ I ZL L1g i b I Water 4 ~ I J 1 ~m Diluent S Z IC) 4) 6 7 I Tot&s g 0 ~ ~ r r ~ ~ -v- v- -r U 2 ~ r 4 (0 I~I 4.~4 ~ ~ alti 6.25% 4 J~ II Li ~i ~ ~ t~ ~ Z~Z~Z]EiZzi~~- vo -~ 6 ~_ p iti~ 7 Tot~ Notes: Page 14 of 50

72 NEW ENGLAND BIOASSAY - CHRONIC TOXICiTY TEST BROOD DATA SHEET Page 2 of 2 FACILITYNAME & ADDRESS: West Valley Demonstration Project WSNPOO7, Rock Springs Rd West Valley, NY NBa FROJECTNUMBER:05 ObLjftJ;Lj~~~ ORGANTSM: Ceriodaphniadubia ~ STARTDATE: Effluent Day Tctal Nuinbei Analyst - Analyst - concentration Number Replicate Live Live Transfes Counts A B C 0 E F G H ~T~TT i T T T T T T T ~T r T 2 -~i ~ T T T T V ~r (ftq 3~~ ~ 7EZJLi~1~~EEE (~ lb ft 125% 4 J~J J7s :~ ~ N Totals ~ T T r r r T T T -r r 2 1 ~Y zr- v r v- ~ -~ -r -~ lb ~ Z@Z~ ~ ILl - I 25% fi_ to ~ (p ~ : II! Totals ~ a (A~ 0 -q T -i ~T / 1 ~ 1t I 1 r ~ r ~ ~- - --~ --r L.. 2 T zr- T r T T ~ r I. ~~ iii (t [~ 50% 4 ci ij.i~a lb V vli3v(t~ io St 5 ~IIJZ±QL~h 6 7 ~ T~s 1 r~rr~r-7----~-- tb is 2 T g -r It? H ~ 77~5~T7~3~t (o6 ~ 100% 4 qq zr11 u iav via v 5k5. 4:~ LWL ~ ~ : C~L~iLLI~L~Ip9 TotaIs~q Page 15 of 50

73 CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,SO 5K IS NOT CALCULABLE SPEARMAN-KARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (%) D D D THE BINOMIAL TEST SHOWS THAT AND tinfinity CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LC5O FOR THIS DATA SET IS GREATER THAN WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE ANY STATISCALLY SOUND RESULTS. DATE: 3/10/12 TEST NUMBER: a DURATION: 48 h SAMPLE: West Valley 007 SPECIES: C.dubia METHOD LCSO CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ~ *~***** ******* MAA ******* ******* ******* ******* PROBIT ******* ******* ******* ******* SPEABMAN.000 ~ ******* ******* = LIMIT DOES NOT EXIST Page 16 of 5D

74 MINIMUM REQUIRED TRIM IS TOO LARGE: 97.5,SO SK IS NOT CALCULABLE SPEARMAN-KARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (%) D D+01 25, D D D 01 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LC5O FOR THIS DATA SET IS GREATER THAN WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE ANY STATISCALLY SOUND RESULTS. DATE: 3/10/12 TEST NUMBER: a DURATION: 7 days SAMPLE: West Valley 007 SPECIES: C.dubia METHOD LC5O CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ******* ~ ******* ******* ******* ******* ******* PROBIT ******* ******* ******* ******* SPEARMAN.000 ******* ~ NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING. ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION. = LIMIT DOES NOT EXIST Page 17 of 50

75 CETIS Analytical Report Report Date: 26 Mar-12 08:07 (p1 of 2) Test Code: a J Ceriodaphnia 7-d Survival and Reproduction Test Analysis ID: Endpoint: N Survival Rate CETIS Version: CETISv1 8.4 Analyzed: 26 Mar-12 8:07 Analysis: STP 2x2 Contingency Tables Official Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 10 Mar-12 10:25 Protocol: EPN821/R (2002) Diluent: Ending Date: 16 Mar-12 09:25 Species: Cerlodaphnia dubla Brine: Duration: Sd 2311 Source: In-House Culture Age: <24h Sample ID: Code: 600F52F8 Client: TestAmerica Sample Date: 09 Mar-12 Material: Industrial Effluent Project: Receive Date: 10 Mar-12 Source: West Valley Demonstration Project (NY000 Sample Age: 34h Station: Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU Untransformed C >T NA NA 100 >100 NA Fisher ExactlBonferroni-Hoim Test control vs C-% Test Slat P-Value P-Type Decision(a:5%) Dilution Water Exact Non-Significant Effect Exact Non-Significant Effect Exact Non-Significant Effect Exact Non-Significant Effect Exaot Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Control Resp NL Yes Passes Acceptability Criteria Data Summary C-% Control Type NR R NR + R Prop NR Prop R %Effect 0 Dilution Water % % % % % % 7d Survival Rate Detail New England Bioassay C-% Control Type Rep I Rep 2 Rep 3 Rep 4 Rep 5 Rep 6 Rep 7 Rep 8 Rep 9 Rep 10 0 DilutionWater d Survival Rate Binomials C-% Control Type Dilution Water RepI Rep2 Rep3 Rep4 RepS RepS Rep7 Rep8 RepO Replo 1/1 0/1 1/1 1/1 1/1 1/1 1/1 1/1 Ill 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/ /1 0/1 in iii ui 1/1 1/1 111 i/i ill 1/1 1/1 1/1 in ui In in in ui u/i 1/1 1/1 1/1 1/1 In 1/1 ill 1/ /1 1/1 1/1 iii ui in 1/1 1/1 1/I ui in CETISTh vl.s.4.24 Analyst: Ok Page 18 of 50

76 CETIS Analytical Report Ceriodaphnia 7-cl Survival and Reproduction Test Report Date: Test Code: 26 Mar-12 08:07 (p2 of 2) a I New England Bioassay Analysis ID: Endpoint: 7d Survival Rate CETIS Version: CETISvt8.4 Analyzed: 26 Mar-12 8:07 Analysis: STP 2x2 Contingency Tables Official Results: Yes Graphics ( Os I (25 25 m I CETISTh vi Analyst QA Page 19 of 50

77 CETIS Analytical Report Report Date: 26 Mar-12 08:07 (p1 of 2) Test Code: a J Ceriodaphnia 7-cl Survival and Reproduction Test Analysis ID: Endpoint: Reproduction CETIS Version: CETISv1.8.4 Analyzed: 26 Mar-12 8:07 Analysis: Nonparametric-Control vs Treatments OfficIal Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 10 Mar-12 10:25 Protocol: EPAI82I/R (2002) Diluent: Ending Date: 16 Mar-12 09:25 Species: Cerlodaphnla dubla Brine: Duration: Sd 23h Source: In-House Culture Age: <24h Sample ID: Code: 600FS2FB Client: Test America Sample Date: 09 Mar-12 Material: Industrial Effluent Project: Receive Date: 10 Mar-12 Source: West Valley Demonstration Project (NY000 Sample Age: 34h Station: New England Bioassay Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Untransformed NA C>T NA NA 100 >100 NA % Steel Many-One Rank Sum Test Control vs C-% Test Stat Critical Ties DF P-Value P-Type Deolslon(a:5%) Dilution Water Asymp Non-Significant Effect ,9590 Asymp Non-Significant Effect ,0000 Asymp Non-Significant Effect Asymp Non-Significant Effect Asymp Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAG Limits Overlap Decision Control Resp 27,2 15- NL Yes Passes Acceptability Criteria PMSD Yes Passes Acceptability Criteria ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(u:5%) Between Non-Significant Effect Error Total Distributional Tests Attribute Test Test Stat Critical P-Value Decision(o:1%) Variances Bartlett Equality of Variance Unequal Variances Distribution Shapiro-Wilk W Normality < Non-normal Distribution Reproduction Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max Std Err CV% %Effect 0 Dilution Water % 0.0% M4 32, % % 12S _as , % -9.93% % % % % % -9.93% Reproduction Detail C % Control Type Rep I Rep2 Rep3 0 Dilution Water , Rep Rep Rep Rep Rep Rep I CETIS1 4 vi Analyst: QA: Page 20 of 50

78 CETIS Analytical Report Report Date: Test Code: 26 Mar-12 08:07 (p 2o1 2) a I Cerlodaphnia 7-d Survival and Reproduction Test - New England Bioassay Analysis ID: Endpoint: Reproduction GETIS Version: CETiSvl.8.4 Analyzed: 26 Mar-12 8:07 Analysis: Nonparametric-Controi vs Treatments Official Results: Yes Graphics 40 S Ii h -S -IC 20 I, I, I I I I 0 G~0I CO CETISW vi Analyst Page 21 of 50

79 CETIS Analytical Report Report Date: 26 Mar-12 08:07 (p1 of 2) Test Code: a I Cerlodaphnia 7-d Survival and Reproduction Test Analysis ID: Endpoint: Reproduction CETIS Version: CET1SvI.8.4 Analyzed: 26 Mar-12 8:07 Analysis: Linear interpolation (ICP1N) Official Results: Yes Batch ID: Test Type: Reproduction-Survival (7d) Analyst: Start Date: 10 Mar-12 10:25 Protocol: EPA/821/R (2002) Diluent; Ending Date: 16 Mar-12 09:25 Species; Ceilodaphnla dubia Brine: Duration: 3d 23h Source: n-house Culture Age: <24h Sample ID: Code: 6ODFS2FB Client: Test America Sample Date: 09 Mar-12 Material: ndustrlai Effluent Project: Receive Date: 10 Mar-I 2 Source: West Valley Demonstration Project (NY000 Sample Age: 34h Station: New England Bioassay Linear Interpolation Options X Transform Y Transform Seed Resamples Exp 95% CL Method Linear Linear Yes Two-Point interpolauon Test Acceptability Criteria Attribute Test Stat TAG Limits Overlap Decision Control Resp NL Yes Passes Acceptability Criteria Point Estimates Level % 95% LCL 95% UCL TU 95% LCL 95% UCL lc5 >100 N/A N/A <1 NA NA 1010 >100 N/A N/A <1 NA NA 015 >100 N/A N/A <1 NA NA 1020 >100 WA N/A <1 NA NA 025 >100 WA N/A <1 NA NA 1040 >100 WA N/A <I NA NA 1050 >100 N/A N/A <1 NA NA Reproduction Summary Calculated Variate C-% Control Type Count Mean Mm Max Std Err Std Dev CV?. %Effect 0 Dilution Water % 0.0% t % % % -9,93% % % % % % -9.93% Reproduction Detail C-% Control Type Repi Rep2 Rep 3 Rep 4 0 Dilution Water Rep CETIS~ vl Analyst: Page 22 of 50

80 CETIS Analytical Report Cerlodaphnia 7-d Survival and Reproduction Test Report Date: Test Code: 26 Mar-12 08:07 (p2 of 2) a New England Bioassay Analysis ID: Endpoint: Reproduction CETIS Version: CETISvI.8.4 Analyzed: 26 Mar-12 8:07 Analysis: Linear Interpolation (ICPIN) Official Results: Yes Graphics 35 F L~ - -c 30 / ~8-~~ ZS I 30 - I, CS CETIS ~ vi.a4.24 Malyst: QA: Page 23 of 50

81 NED i DATA S1~ET FOR ROUTINE CEEMXCAL AND PHYSICAL DETERMINATIONS hgel of 2 ~ FACILITYNAME &ADDRBSS: West Valley Demonsttntion Prnject WSNI 007, Rook Springs Rd West Valley, NY NEB PROJECT NUMBER: 0 bgq 2!-tO CL) TEST ORGANISM: Ceriodaphnia dubla DILUTION WATER SOURCE: Receiving Water START DATE: 5 ~D I i ~. j TIME: O ANALYST ~ me7 C~- ()~ ~),,~V NEBLab REMARKS Synthetic Conttvl Temp., C Initial 15-9 ~b5 ~4. T ~$2 &S a Z~fi ç D.O.,rng/L Initial ~ro ~( 7E ~.b %.a ~ t4 ph,s.u. Initial ~g R~t) 7q 7.6 7~ Conductivity, U~ Initial ~ c~9oz ~ e3~5o.353 ~qç Temp., C Final?J(-.o CQLP.O ~2~-h I Vt- 1 Z4~ ~.J D.O.,mg I, Final PltS,u. Final ~ gq J) ~ Conductivity~gS Final ~ cf,3 ~7 3~Lf 2~s-j ~~) I Receiving WaterDiluent I REMARKS Temp., C Initial ~ 195Lp ~-%e cq- +7 2(sS) ~t1i3 D.O~rnWL Initial 9o 9(~ ~? I io.ik q~t.( pn,s.u. Initial q~ ~ ( 75 ~4 Conductivity, ~ts Initial ~gz~ 339- cz~jp ~ 7 &J3 ~tc~ Z Temp., C Final z ~. ~ 2~O ~q-~ -vie v~ ~ D.O~mg L FIn~d ~-,3 ~7 ~.5 ~ ;/ ph,s.u. Final ~p S.C)?O g, I t-c) Cnnduclivity,[LS Final ~s st~ ~Q 6.25% REMARKS Temp, C Initial ZQ 1 ~5th J2.4o &.qs ~.c ~C.2~o D.O.,rng/L Initial 1b27- ~ gj,~ gi. 9J1? Gt,,(4 ph,s.u. Initial 7,ç ]to 77 VT ~7-7 -l~ c ConducUvit~ ~ts Initial LtL (0 381 (5F~24 ~ a0s ~-cq Temp., C Final iqs oqo 2 -l ~7 zt1~ Ci ZLL, s zc / D.O~mg/L Final %1 6.4?cY 3~ c g-~ ph,s.u. Final 3.f iuo g.j t-z ~1Z Conductivity,gS Final q~3 (4/3 ti~cko LtL[ St % I REMARKS Temp., C bikini zs~o 85.Lo Mo ~QA44 QCa.O 3~i~ D.O.,mg L Initial tc-s ~7 9,b ~ 9.t g~ç ph,s,u. Initial 7& b7(~4) V/ 11 7.~ c. Conductivity, p5 Initial L/(ac c-fu3 2f/4 s (pqs C?)? Temp~ C Final ~t~ê ~ ~ flr Z&~-Z D.O.,mgIL Final ~ 3:6 as y- y ~ 9-b ph,s.u, Final I Z~ ~ & -3 3R- 6~3 ConductivibstaS Final t~3q LjcjLJ c-ko3 t/7y - c~2 ~ Page 24 of 50

82 NEB s DATA SHEET FOR ROUTINE CHEMICAL AN]) PHYSICAL DETERMINATIONS Pagel of 2 FACILYrYNAME & ADDRESS West Valley Demonstiation Fmject WSNPOO7, Rock Spnngs Rd West Valley, NY NB PROJECTNUMBER: ~-ç. ~L4~tiQ~oO TEST ORGANISM Ceriodaphnia dubia DILUTION WATER SOURCE: Receiving Water STARTDATE ITIME:! O~ 25% REMARKS Teinp, C thitial ~ &6Lo ot4ô ~ LO ZLi.t D.O.,mg L Initial Lo ~ g~ ~q q.~ q~ ph,s.u. Initial q /.~ 77 tt ms 7/7 ConducUvi~S Initial Ws 1& Temp., C Final zq 3 2LAQ a5~o ~ct 2t74 2S~( D.O.,mg/L Final 8~3- ~~C) &S 8 -~ ~7 3~4 ph,s~ Final 35 ta. 1.1 ~-..LJ Conductivity, 45 Final (~ 7~ (~3 a (~oc~ Q~3 (2 ~ 1C ~ ([7 50% I REMARKS Tetnp., C Initial z~-g â54 clt/.n &45 ac.s 7t4tO D.O.,mg/L Initial 21. g~> ~ b.0 g~c~ ph,sal Initial 7X ry qg 7~C( 711 ConductMt~yiS Initial 830 &% Sbm 8(o7 ICY? i bce / Temp., C Final ZL(,(ç, 2t1.O 25.~ a5-~) Z4c 25-2 D.O.,ingfL Final ~ 7%~ 9.0 %.Q 3~ j ~Lf ~ -t~ ph,su. Final s~s ~ ~ a 8- J 33 ConductiviW~sS Final S6S gqy- 8Z~+ 39 I I ~ )jl.j3 100% REMARKS Teinp., C Initial 2~s 6 a~l cq4.o c32j-(7 a&~ 3L40 Dfl,mg)L Initial 9~ &.S ~ qo II. /0.0 pm,s.u. Initial ~ 7q #77 7C7 3 Q 7~f ConductivitMiS Initial 7r 1373 h29(0,,5?& RQ~3 j Tanp., C Final Z4G flo ~a Zs-Z- Z ~tg z5-3 ao., mg/l Final R-3 1~7 t-s q. C ph,s.u. Final Vt 5-i i.~ Conductivity, ss Final 35O L.3z5 I qao I 3~- i ç.cco ~ Temp., C Initial REMARKS DO., mg/l p1-i, s.u. Initial Initial Conductivity, ItS Initial Temp., C DO., rng/l ph, sn. Final Final Final Conductivity, ~LS Final Page 25 of 50

83 Cerioclaphnia dubía Culture Chart ~ Brood mother source: 2M&) C jq ~ Sources brood size: /7 ~I~1~c ~ ~ate~ft~ z~% (Qty.)~ Lot# 030 We9~ g0q.&)zlbo Day aco. ~i ~ 2NNW Yw2y \ ~ A ~t: y ~ ~ ~ c ~ 8NN N V ITS A/8 y ~ ~ 5N N I / / 5 I w 9NN / 9 / WN N 1 / ~ ~ \ \ flnn~ N N V T ~ 7 r~ v N N / 13 1 Yneonates present X = brood mother dead neos present mid-day of preceding day (see Nine in log) 2Y = two broods ae = aborted eggs P = nec s present in P.M. of preceding day (sae time in log) N = no neonates soc. = acclimation (H20 type used w/ renewal this day) = neonates used in test, rep. if noted (and brood counted). Test organism collection: Tray diagram used? Project# lest ID # Symbols ( P) (YIN) Time period, neonates released Colleotion date! time Oo~qt~o T 2-?i~/ 5a5 ) ~ ~-)e/ J/~3 OOQ97~7 7 ~-7-l~/ o5a~ ) Ii;o ~~-/a v T T Page 26 of 50

84 Ceriodaphnia dub/a Culture Chad Lot # Cd ia_4 tin Brood mother source: ~H HO/C! ~ Source s brood size: (Qty.) West V~IIey [mch[~ ~tci ~ D&e % t/~ ~)3 7 ~ ~ 1/q Day ccc. ~ IN NN 7 ~ ~ YTY 2 N N P1 ~ y~j /3g 3 N N N 7/ 4 N7 N 7 / ~ / 4 jn N ~AJ 6N N 7 Y ~ / ~ ill ~NNO 8 N N w Y /~(/8V r~ 7 9N N 1 10 N N 11 N N fln N H 13 N N 7/ 10 v ti / / 10 K 1o { f Y ts ~ f J i~ It, 4 ill ~7/~,v/ ii v ~ / N Y = neonates present X = brood mohier dead,/ nec s present mid-day of preceding day (see time in log) 2Y = two broods ae = aborted eggs P = neos present In P.M. of preceding day (see time in log) N = no neonates acc. = acclimation ([1~O type used wi renewal this day) T# = neonates used in test, rep. # noted (and brood counted). Test organism collection: Tray diagram used? Project # Test ID # Symbols (4 P) (V/N) Time period, neonates released Collection date I time O0~9~O T ~ ~-7~j~ /o5~s ~ )I~o Q~L~7~ 0 ~ d915-1/3o, T T~ T T Page 27 of 50

85 MS S SURVWAL DATA SHEET FOR FATHEAD MINNOW LARVAL SURVIVAL AND GROWTH TEST Page 1 oil FACILITY NAME & ADDRESS: West Valley Demonstration PiDject WSNPOO7, Rock Springs Rd West Valley, NY NEB PROJECTNUMBER l at~lt -(DO NEB TESTNUMBBR:1 LjL~\, COCiLi~2 I2bC~ Lcn TEST ORGANISM: Pimephales promelas AGE: <24 horns LCff #: I Ca ~ 6 ) STARTDATE: Sj,uO I, ~ a IT1~ ENDDATE: J7 j )ç9~ TIME: Number of Survivois Effluent Replicate Day Concentration Number j Remarks ANALYST NEBLa1 A 10 I D Ic to t. ~4) to lo Synthetic B 10 Lv to io Lc Lt LI) Contmi C 10 lcd 1L 10 1,0 1) 10 It) CO ii) K) ii) ii) to Receiving A 10 ~Q ~n to to U Water B Diluent C 10 IJCJ D 10 1K) ~C) IC) tc) (C~ to A ~V-H Cf 6.25% B 10 (0 tti ~C) (t t~ LO CO C 10 to I@j LC) i,c> ID (b (6 9 A 10 In ~o VO LO in (0 12.5% B 10 i o t.o 1c to lb ic C 10 1k) là 0 10 ic It it Lo A to lb ID UN l~t~ 25% B (C) to ib ID CD C lb L~ to ID 1 ) LI~ ~ to U) U~) lb 9t ~~ A 10 (C) ftt ROLD U) ID (C) 50% B 10 Lb U) to to ID C 10 to l.d UDin ID it 0 10 ir 0 10 [0 1.0 (0 il) A 10 ~~ç j UP ~O 10 (13 IC) Li) 100% B 10 IC) I~) Ct) [P iç~~ to C 10 \j) 10 lb [c) 1~ ~i 9 D 10 I.e 10 lid to U) 1(~ (C) C DO concentration fell below 4.0 mg/l C All test solutions were aerated at <100 bubbles/minute as of Page 28 of 50

86 CT-TON: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARI4AN METHODS MINIMUM REQUIRED TRIM IS TOO LARGE: 99.5,SO SM IS NOT CALCULABLE SPEARMAN KARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (%) D O95D 1O O95D 1O O95D 1O O95D 10 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LCSO FOR THIS DATA SET IS GREATER THAN WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE ANY STATISCALLY SOUND RESULTS. DATE: 3/10/12 TEST NUMBER: b DURATION: 48 h SAMPLE: West Valley 007 SPECIES: P.promelas METHOD LCSO CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ~ ~ MAA ******* ******* ******* PROBIT ******* ***-**** ******* SPEARMAN.000 ******* ~ ******* NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING. ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION. LIMIT DOES NOT EXIST Page 29 of 50

87 MINIMUM REQUIRED TRIM IS TOO LARGE: 97.0,SO 3K IS NOT CALCULABLE SPEARMAN-EARBER TRIM:.00% LC5O: % CONFIDENCE LIMITS ARE UNRELIABLE. CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. 3) D D 10 25, D D D 08 THE BINOMIAL TEST SHOWS THAT AND +INFINITY CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS PERCENT. THE LC5O FOR THIS DATA SET IS GREATER THAN THE MOVING AVERAGE METHOD CANNOT BE USED WITH THIS DATA SET BECAUSE NO SPAN WHICH PRODUCES AVERAGE ANGLES BRACKETING 45 DEGREES ALSO USES TWO PERCENT DEAD BETWEEN 0 AND 100 PERCENT. NO CONVERGENCE IN 25 ITERATIONS. PROBIT METHOD PROBABLY CAN NOT BE USE WITLI THIS SET OF DATA. DATE: 3/10/12 TEST NUMBER: b DURATION: 7 days SAMPLE: West Valley 007 SPECIES: P.prornelas METHOD LC5O CONFIDENCE LIMITS LOWER UPPER SPAN BINOMIAL ******* ~ MAA ******* ******* ****9r** ******* PROBIT ******* ******* ******* ******* SPEARMAN.000 ~ ******* ******* NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING. ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION. = LIMIT DOES NOT EXIST Page 30 of 50

88 CETIS Analytical Report Report Date: 26 Mar-12 08:23 (p 1 of 4) Test Code: I 2-443b I Fathead Minnow 7-cl Larval Survival and Growth Test Analysis 0: Endpoint: 7d Survival Rate CETIS Version: CETISv1.8,4 Analyzed: 26 Mar-12 8:21 AnalysIs: Parametric-Control vs Treatments Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: 10 Mar-12 11:00 Protocol: EPPJ82I/R (2002) Diluent: Ending Date: 17 Mar-12 12:15 Species: Pimephales promelas Brine: Duration: 7d lh Source: In-House Culture Age: <24h Sample ID: Code: 6ODF52FB Client: Test America Sample Date: 09 Mar-12 Material: Industrial Effluent Project: Receive Date: 10 Mar-12 Source: West Valley Demonstrahon Project (NY000 Sample Age: 35h Station: New England Bioassay Data Transform Zeta Alt 1-lyp Trials Seed NOEL LOEL TOEL TU PMSD Angular (Corrected) NA C> T NA NA 100 >100 NA % Dunnett Multiple Comparison Test Control vs C-% Test Stat CrItical MSD DF P-Value P-Type Declslon(o:5%) Dilution Waler CDF Non-Significant Effect CDF Non-Significant Effect CDF Non-Significant Effect CDF Non-SIgnificant Effect CDF Non-Significant Effect Test Acceptability Criteria Attribute Test Stat TAC Limits Overlap Decision Control Resp NL Yes Passes Acceptability Criteria ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decislon(cc5%) Between Non-Significant Effect Error Total Distributional Tests Attribute test Test Stat Critical P-Value Decision(a:1%) Variances Mod Levene Equality of Variance , Equal VarIances Variances Levene Equality of Variance Equal Variances Disfrlbution Shapiro-Wilk W Normality ,0318 Normal Distribution 7d Survival Rate Summary C-% Control Type Count Mean 95% LCL 95% IJCL Median Mm Max Std Err CV% %Effect 0 Dilution Water l % 0.0% % 5.26% I 0 0.0% -5.26% S I % -2.63% % -5.26% I % -2.63% Angular (Corrected) Transformed Summary C-% Control Type Count Mean 0 DIlution Water & % LCL t t412 4 t371 1, t412 4 t % UCL 1.48 lass L Median t412 t t412 Mm Max , Std Err CV% 7.07% 9.94% 0.0% 5.94% 0,0% 5.94% %Effect 0.0% 5.73% -6.12% -3.06% -6.12% -3.06% I CETISTM vl Analyst: QA: Page 31 of5o

89 CETIS Analytical Report Report Date: Test Code: 26 Mar-12 08:23 (p2 of 4) b I Fathead Minnow 7-d Larval Survival and Growth Test - New England Bioassay Analysis ID: EndpoInt: 7d Survival Rate CETIS Version: CETISvI.8.4 Analyzed: 26 Mar-12 8:21 Analysis: Parametric-Control vs Treatments Official Results: Yes 7d Survival Rate Detail C-% Control Type Rep I Rep 2 Rep 3 Rep 4 0 Dilution Water , Angu!ar (Corrected) Transformed Detail C-% Control Type Rep I Rep 2 Rep 3 Rep 4 0 Dilution Water , d SurvIval Rate Binomiais C-% Control Type Rep I Rep 2 Rep 3 Rep 4 0 DilutIon Water 10/10 9/10 9/10 10/ /10 10/10 9/ /10 10/10 10/10 10/ /10 10/10 10/ /10 10/10 10/10 10/ /10 9/10 10/10 Graphics 0, vs L.,J 0.05 p , v-i vs 0, ,0 03 is 4.5 0, Si kilo CETIS~ vl Page 32 of 50 Analyst

90 NEW ENGLAND BIOASSAY - WEIGHT DATA FORFATHEAD MINNOW LARVAL SURVIVAL AND GROWTH TEST Facility Name and Address: WestValley DemonstmtionProjectWSNP0O7, Rock Springs Rd West Valley, NY NEB Project Number: os. a (~ L4t4 ~~4O ~ NEB rest Number: Q Testflates: )~ ~ ~ ~ weigl~ngdate 3)as)\~9. Diying Temperature ( C): 100 Diyhig Time (homs~: 6 Analyst Initial Weights (A): (~ &~, ~j 17f I Analyst Final Weights (B): ~ A B Effluent Concentration Replicate Number Weight of boat (mg) Dry weight: foil and Ian ae (rag) A NEBLab B 0}5(QaS SyntheticControl C gi4g ) ~ 955 ~ 3 A ci5&o~3., Receiving Water B Qc~4q ~ Diluent C 7 625% B A C A 9Y.i~gq q~p-iq 12.5% 3 c1~3,o1 C D gry~g3. A 25% B ~ QTc.~ T D grfl0~ 50% A jog iia. i / B 9~3. go C - ~t*(-- ~Lvas% D 9(~O3 A 100% B 9ss.ck~ C ~94t~41 D ~9?4D l.c/.- WI&& Page

91 CETIS Analytical Report Report Date: 26 Mar-12 08:23 (p 3 of 4) Test Code: b Fathead Minnow 7-d Larval Survival and Growth Test Analysis ID: Endpoint: Mean Dry Weight-mg CETIS Version: CETISvI.8,4 Anal3/zed: 26 Mar-12 8:22 Analysis: Parametric-control vs Treatments Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: IC Mar-12 11:00 Protocol: EPP1821/R (2002) Diluent: Ending Date: 17 Mar-12 12:15 SpecIes: Pimephales promelas Brine: Duration: 7d lii Source: In-House Culture Age: <24h Sample ID: Code: 600FS2FB Client: Test America Sample Date: 09 Mar-12 Material: industrial Effluent Project: Receive Date: 10 Mar-I 2 Source: West Valley Demonstration Project (NY000 Sample Age: 35h Station: New England Bioassay Data Transform Zeta Alt Hyp Trials Seed NOEL LOEL TOEL TU PMSD Untransformed NA C >T NA NA 100 >100 NA % Dunnett Multiple Comparison Test Control vs C-% Test Stat Critical MSD DF P-Value P-Type Declsion(a:5%) Dilution Water ,7026 CDF Non-Significant Effect ,8449 CDF Non-Significant Effect 25 0, CDF Non-Significant Effect CDF Non-Significant Effect CDF Non-Significant Effect ANOVA Table Source Sum Squares Mean Square DF F Stat P-Value Decision(a:5%) Between ,2844 Non-Significant Effect Error Total Distributional Tests Attribute Test Test Stat Critical P-Value Decision(a:1%) Variances Bartlett Equality of Variance Equal Variances Distribution Shapiro-WilkW Normality 0, Normal Distribution Mean Diy Weight-mg Summary C-% Control Type Count Mean 95% LCL 95% UCL Median Mm Max Std Err CV% %Effect 0 Dilution Water A % 0.0% A % 2.21% % -0.24% % 1.68% % -0.96% % % Mean Dry Weight-mg Detail Control Type Dilution Water Rep I Rep Rep Rep ( I CETISTh vi Analyst Page 34 of 50

92 CETIS Analytical Report Fathead Minnow 7-d Larval Survival and Growth Test Report Date: Test Code: 26 Mar-12 08:23 (p4 of 4) b New England Bioassay Analysis ID: EndpoInt: Mean D~y Weight-mg CETIS Version: CETISv1.8.4 Analyzed: 26 Mar-12 8:22 AnalysIs: Parametric-Control vs Treatments Official Results: Yes Graphics ~ z 04 0*0000 0J CV ~ LI IS kilo CETIS 4 vi Page 35 of 50 Analyst CA

93 CETIS Analytical Report Report Date: 26 Mar-12 08:23 (p1 of 2) Test Code: b I Fathead Minnow 7-d Larval Survival and Growth Test Analysis ID: Endpoint: Mean Dry Weight-mg CETIS Version: CETISvI.8,4 Analyzed: 26 Mar-12 8:22 Analysis: Linear Interpolation (ICPIN) Official Results: Yes Batch ID: Test Type: Growth-Survival (7d) Analyst: Start Date: 10 Mar-12 11:00 Protocol: EPN82I/R (2002) Diluent: Ending Date: 17 Mar-12 12:15 SpecIes: Pimephales promelas Brine: Duration: 7d lh Source: In-House Culture Age: <24h Sample ID: Code: 60DF52F8 Client: Test America Sample Dale: 09 Mar-12 Material: Industrial Effluent Project: Receive Date: 10 Mar-12 Source: West Valley Demonstration Project (NY000 Sample Age: 35h Station: Linear Interpolation Options X Transform Y Transform Seed Resamples Exp 95% CL Method Linear Linear Yes Two-Point Interpolation Point Estimates Level % 95% LCL 95% UCL TU 95% LCL 95% UCL CS >100 N/A N/A <1 NA NA lcio >100 N/A N/A <1 NA NA IC1S >100 N/A N/A <1 NA NA lc2o >100 N/A N/A <1 NA NA 1C25 >100 N/A N/A <1 NA NA 1C40 >100 N/A N/A <I NA NA lc5o >100 N/A N/A <1 NA NA Mean Dry Weight-mg Summary Calculated Variate C-% Control Type Count Mean Mm Max Std Err Std Dev CV% %Effeot 0 Dilution Water , , % 0.0% % 2.21% , % -0.24% , % 1.68% , ,26% -0.96% ,98% % Mean Dry Weight-mg Detail Control Type Dilution Water Rep I 0, Rep ,557 0,607 Rep Rep , New England Bioassay CETIS vi Analyst Page 36 of 50

94 CETIS An&ytical Report Report Date: 26 Mar-12 08:23 (p2 of 2) Fathead Minnow 7-cl Larval Survival and Growth Test Test Code: Analysis ID: Endpoint; Mean Dry Weight-mg CETIS Version: CETISvI.8.4 Analyzed: 26 Mar-12 8:22 Analysis: Linear Interpolation (ICPiN) Official Results: Yes Graphics I2-443b I New England Bioassay ICC CETIS~ vl.&4.24 Analyst QA: Page 37 of 50

95 Final Weight Tare Weight Fish Weight weight/lu Mean , , Page 38 of 50

96 NEB edata SKEET FOR ROUTINE CHEMICAL AND PHYSICAL DETE1~MD{ATION5 ± ofj ~ FACILWY NAME & ADDRESS: Westvalley DemonstrationpiojectWSNPO07, Rook Springs Rd Wet Valley, NY NEB PROJECT NUMBER: ~ estsqlj~ LI&_~D TEST ORGANISM: Pimephales prornelas DILUTION WATER SOURCE: Receiving Water START DATE: ~j [0.~ TIME: I tv~ ANALYST ~flrfl3c~, (3~ YD MVó~ NEBLab REMARKS Synthetic Control Ternp~0C Ithial 3~j a~5 PtT ~ -ks ~ 24~ c O?S~.S~ IXO.,inglL Initial ~ r7-q c35 ~,9., t~ 8s ph,s.u. Initial j~ 3~ 7t7 73 ~S?~LQ Conductivity,pS Initial S ~t3 ~93 3L) :35-( Ternp~ C Final ~ C~/9-.O2 &L(.,5 24~ 3 2~(7 1 [Y 3 J247 ao,,ing/l Final 9o ~1ij t. [.tf G7 (~ 3 ta,& ph,s.u. Final 77 7~ -7~ ~7.7 In ~ 3? y,g Conductivit.stS Final ~Ø &-4~ p~3 3?q 37o 3~C 371 Receiving Water Diluent REMARKS Ten~p., C Initial Z5-O 95L4,2?4.O ~J{ç ~o zlii 3 ~2&o D.O~rng/L Initial j~ cflp ~N 10$ c~k ph,s,u. Initial ~ 7(7 7~- ~rr i-s 74 YIp Conductivity, ~LS Initial LJ~ c2&p 3&T Te:np., C Final 7tj10 >245 Qi4,~~ 1&0.jçtj Z~ 3. ~4 ~ D,O,,rng L Final ~5 ~.\ t~/~7 Q,L1 c~g (pif ph,s.a Final ~j? r7~ 75 ~7-C Vi ~G 72 Conductivity, 118 Final ~ 8 ~ o35 cv i 61,5 5 I I 6.25% REMARKS Ternp., C Initial ~q9 ~ ~ c2+(j 0fl3 9c~.o ZL~(LC) op (AG D.O.,mg/L Initial j(7.7 %1,7 9-(c 9.~- ~ ph,s,u. Initial 7~ 7~(~ TI ~ m7 ~ c- rt~ Conductivity, ILS Initial q. ~ ~ &5&i. ~ 5( g 53- c c r- ~ Tetnp,, C Final 2L~1~Y ~4s ~I4Lf zs-o j~fl z-q-q 9JfJ0 D.O.,mg/L Final ~ s.7 ~i 50 ph,s,u. Final 7,(~ 71o.7 s 7-G 77 Conductivity,~LS Final L17fl 3cj7 acs ~* s6 7 5?O 1 53~ 12.5% REMARKS Temp~ C Initial 25-0 ~ ~24o ~4 ajlo Z14 / c~~/p~j D.O.,mg/L Initial ICC &~7 9.3 z q~ 9S ph,s.u. Initial ~~j(~ 7-1 ~ 7T ~-7?C TI Conductivity, ItS Initial ( (0ç Q~ç3 1-j/4.&147 (~ ~ts c z t~ ~ Teinp,, C Final z41.e o.v-i.7 ~{4 ZG-6 zcm z~&-3 &47 D,O~ing/L Final ~99 L]~ ~ I CeS Le,5 pli,s.u. Final.77 7L~ 9.(~ 7- J 77 7,9 Conducthit~S Final ~ L.j~i4Q QOCo L-j(ç3 Quit C~3 (AS Page 39 of 50

97 NEB idata SHEET FOR ROUTINE CHEMICAL AND PHYSICAL DETERMINATIONS Page 1 of 2 P FACILErY NAME & ADDRESS: West Valley Demonstration Project WSNPOO7, Rook Springs Rd West Valley, NY NEB FROJE~F NUMBER: oc O b 147) TEST ORGANISM: Pimephakspromelas DILUTION WATER SOURCE: Receiving Water STARTDATE: & j~c> I ~ TIME: I. LOC 25% REMARKS Temp. C InWal 751 ~7~(~ ~4() J4LJ~S3 ~Q ~-jco 0~Le.O ao.,rng/l Initial LOS 8.(o 9~o 8.9 CIj 7 q..o ph,s.u. Initial p49 7fl lfl~ 177 7~ Conductivity, gs Initial 6c~ cp44 S isc 7~ Teinp., C Final imt& J~i.\.jp ~-k.6?fa O Zç~ rn~~v &-~cq D.O~mWL Final ~ 1-7 Li.1 ~,c (ttp ph,s,w Final rjc~3 ~l(o ~6 74 ~-~- ~ o Conductivity,~tS Final y4q O (/j 579J (oto 7~t?9L % REMARKS Temj~, C Initial zs-q 95.q-. ~ $c c~5.5 ~J4.O ~~jp~o D.O~ins/L Initial I&.~Z ~,b ~ s.w io.o q,c. q~ p~. Initial ~fl 7~~ T7 r~ ~ ~ 7 s ConductivityjtS Initial g-co ~5(o 800 &ot 1071 IO~ I,1~ p~ Temp., OC Final ZL4t0 &J-~ ~ ~ i~-y 15(7 7}(. (If ~Wp(p Ofl,ing/L Final g~ 99~. T9 ~- 3 ~ 3 ph,su. Final ~ ~ i~ R-? rn ~-8 2J ConductivftM.S Final ~ ~7 ~ar 873 ~o joiç ~ ô& ( io(~[ 100% REMARKS Temp., C Initial ~ ~4p ~47 2S.I rj. b?5~s D.O.,nlgI[. Initial ~ 5 S~ Iii (C. 0 ~ ph,s,u. initial ~77, 79?T S.~ Conductivity, ps Initial 7, (y~- J 373 /~p~q(~ /5~7(p ( ~3 o Teinp., C Final ztlo o~4i7 ~4.3 2&-6 zs.c 19?5 Mi D.O~rng/L Fiaal ~ 9~5 17 ~ I 5 (~ç (ml ph, au. Final ~,,~ SO 7-9 ~- o I Conductivity, AS Final g~(~ I~7 P415 1 L~ (p i (:17 I, lot 1 i LI~ ;z I REMARKS Temp., C DO., mg/l pil, sn. Initial Initial Initial Conductivity, AS Initial Temp., C DO., mg/l ph, s.u. Final Final Final Conductivity, p5 Final Page 40 of 50