Carlo Pozza Urban wastewater treatment using Scenedesmussp.

Transcription

1 Carlo Pozza Urban wastewater treatment using Scenedesmussp. Carlo Pozza, Sebastian Schmuck, Thorsten Mietzel Urban Water and Waste Management Universitätsstr Essen

2 2 Presentation summary Project presentation Project progress Conclusions

3 3 Project presentation Wastewater nutrient removal with microalgae. Investigate the use of a novel technology to illuminate the microalgae. Conduct large-scale test using reactor with internal illumination and different light sources. Study the alternative uses of the microalgae after the harvesting (AD, by-products, biofuel).

4 4 Possible applications Nutrient removal Treatment of urban wastewater as secondary or tertiary treatment. Treatment of high polluted wastewater (industrial wastewater, centratefrom sludge thickening from urban wastewater treatment plant, effluent from anaerobic digestion plant, leachate). CO 2 and CH 4 Use of flue gas for enhancing the algal growth (anaerobic digestion plant or power plant). Use of the harvested microalgae for anaerobic co-digestion.

5 5 Potential benefits of microalgae Microalgae grow naturally in wastewater Microalgae remove N and P during their grow (proteins are 45-60% of microalgae dry weight) Assimilation of CO 2 for photosynthesis (about 2 tons CO 2 for 1 ton of microalgae; high tolerance to high CO 2 concentration; the use of flue gas enhance the grow of the microalgae) Synergic effects (O 2 production (bacteria); rising of the ph for metals and phosphorus precipitation, ammonia volatilization) Biomass production (Biogas production, biofuels, fertilizer, animal feed, high value biomolecules production)

6 6 Internal illumination With the internal illumination, the light is spread into the reactor allowing a better distribution. There s the possibility to use different kind of light sources in the photobioreactor(sunlight, LED, fluorescent lamps) Lower surface requirements compared to high rate ponds. Photobioreactor with fibre optics as a source of light Microalgae illuminated with a round bar made of Plexiglas and red and blue LED.

7 7 Progress Experiments Scenedesmus sp. + artificial wastewater Scenedesmus sp. + real urban wastewater

8 8 Batch test in 1-liter reactor with different starting ammonia concentrations (50, 150, 400, 1000 mgn-nh 4 /L) Experiments Fluorescent lamps with solar spectrum, µmol/m 2 /s 20:4 light-dark cycle Temperature: 23 C ph control: 6-7,5

9 9 Experiments Higher removal efficiency and higher growth rate at lower N-NH 4 concentration. The N-NH 4 removal rate is higher, high is the starting concentration. Average removal rates: 8,0 mgn-nh 4 /L/d(1000 mgn-nh 4 /L) 6,0 mgn-nh 4 /L/d(400 mgn-nh 4 /L) 3,0 mgn-nh 4 /L/d(150 mgn-nh 4 /L) 1,8 mgn-nh 4 /L/d(50 mgn-nh 4 /L)

10 10 Experiments A: Urban wastewater + Scenedesmus sp. grown in normal media B:Urban wastewater+ Scenedesmussp. grownwithhigh concentrationof N-NH 4 (800 mgn-nh 4 /L) C: Control reactor, urban wastewater Fluorescent light with solar spectrum PFD: µmol/m 2 /s, 20:4 light-dark cycle Average temperature: 24 C ph control bubbling pure CO 2

11 11 Experiments A: Urban wastewater + Scenedesmus sp. grown in normal media

12 12 Experiments B: Urban wastewater+ Scenedesmussp. grownwithhigh concentrationof N-NH4 (800 mgn-nh4/l)

13 13 C: Control reactor, urban wastewater Experiments

14 14 Experiments FISH test The sample is stained with DAPI and is excited with ultraviolet light: all the cells with DNA are bluecolour. The sample is excited with green light and the algae, in these condition, emit naturally the red.

15 15 Summary Build and test a lab-scale reactorwith internal illumination and afterwards a large-scale reactor. Test of different type of real wastewater and different source of illumination(led, fluorescent lamps, sunlight).

16 16 Thank you for your attention Carlo Pozza, Sebastian Schmuck, Thorsten Mietzel Urban Water and Waste Management Universitätsstr Essen