Chlorophyll Training. If you registered for Chlorophyll-- - get your equipment NOW from Jean (out in the hall) before the training starts.

Transcription

1 Chlorophyll Training If you registered for Chlorophyll-- - get your equipment NOW from Jean (out in the hall) before the training starts.

2 COOPERATIVE LAKES MONITORING PROGRAM TRAINING FOR Chlorophyll-a

3 Paul Steen Huron River Watershed Council 1100 N. Main Street Ann Arbor, MI x601

4 Trophic State Indicators Transparency Total Phosphorus Chlorophyll a Dissolved Oxygen and Temperature Aquatic Plants

5 Chlorophyll a Training Outline What is chlorophyll? Schedule Water Collection Sample Handling preservative (MgCO3) field filtering freezer storage Trophic state indicator Variability and trends

6 CHLOROPHYLL-A What is Chlorophyll-a?

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9 CHLOROPHYLL-A Sampling Schedule

10 micorps.net Lake Monitoring CLMP documents

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12 NEED 4-5 Samples Lakes change over time! Abunance Seasonal Succession of Lake Algae in a Mesotrophic Lake Winter Spring Summer Fall Winter Credit: Water on the Web

13 Chlorophyll Equipment Bag of equipment contains -60 cc (ml) syringe -filter holder -filters (12-13) (in a baggy) -tygon tube -vials with caps (11) -tweezers -amber bottles (2) -dropper bottle with MgCO 3 (labeled) -zip-lock bags -labels (11) -clothes pin Weighted composite sampler Keep a copy of the quick procedures handy

14 Chlorophyll Sampling Re-supply Kit Filters (11-12) with warning label (in envelope) Vials with caps (11) Dropper bottle with MgCO 3 (labeled) Zip-lock bags Labels (11)

15 Chlorophyll Sampling Equipment Provided by volunteer: boating safety equipment anchor pencil or indelible ink pen measured line for sampler freezer ice pack

16 CHLOROPHYLL-A Step 1. Getting a depth integrated water sample

17 Connecting ring Measured line Rubber stopper with 2 glass tubes Retaining chain Juice can Suspension chain Bottle cap One liter amber bottle 2 dumbbell weights

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22 Secchi Disk Measurement NO SUNGLASSES! 1. Disk lowered slowly until it disappears from view 2. Disk raised slowly to point where it reappears 3. Record: the secchi depth is average

23 Depth Integrated Composite Sample Collection Light Penetration Secchi Disk Depth Chlorophyll Sampler Twice the Secchi Depth Extent of Euphotic Zone Or Depth to Which Light Penetrates in a Lake

24 Rinse with lake water

25 Lower to twice the Secchi depth

26 Slowly bring to surface

27 Want bottle 2/3 to 3/4 full

28 Fill the 2 rectangular amber bottles.

29 5 drops of MgCO 3 preservative added to each bottle

30 Cold storage until returning to shore

31 CHLOROPHYLL-A Step 2. Filtering the water sample

32 Chlorophyll Filtering Equipment 60 cc plastic syringe flexible plastic tube filter holder membrane filter disks tweezers sample storage vials and caps (2) chlorophyll sample labels (2)

33 Chlorophyll Filtering Equipment Provided by Volunteer fine-tip permanent black marker aluminum foil large safety pin coffee filter or paper towel zip-lock freezer bag

34 Let s go to the video tape! Filtering section starts at 5:50.

35 Chlorophyll filtering equipment

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37 Remove translucent separator sheets (your filters may not have these)

38 Rinse with sample water then fill to 60cc line

39 Plunge to 50 cc (ml) line

40 Slight pressure

41 Tweezers and safety pin

42 Carefully fold filter paper

43 Put folded filter disk into vial

44 Tin foil to keep out light

45 Freezer storage until turn-in date

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47 Sample turn-in and submitting your data 1. Make copies of your data forms for your records. 2. Include originals with your frozen samples. 3. Turn in frozen-sample per the schedule. 4. Enter your field data into the online MDE by October MDE login: Get it from

48 Common Reasons for Sample Rejection Sample collected at the wrong time Samples collected far outside the assigned interval will be rejected Samples collected on cover slip, not white filter disk Samples not wrapped in foil Incorrect delivery If you forget to turn your samples in to the local DEQ office on the assigned date CONTACT US for instructions on safe shipping. Unexpected shipments will thaw and be rejected.

49 Year End Report

50 What is Trophic Status Index? Avg. Secchi Depth Summer Phosphorus Median Chlorophyll-a x log 10 SD x log 10 TP x log 10 CHL TSI SD TSI TP TSI CHL Averaged Average TSI

51 Trophic Status Low Medium High Very High Oligotrophic Mesotrophic Eutrophic Hypereutrophic

52 Trophic Status Low Medium High Very High Oligotrophic Mesotrophic Eutrophic Hypereutrophic

53 Current snapshot: Trophic Status (2017) Based on full participation in Secchi, Chlorophyll, Total Summer Phosphorus in 2017 N = 128

54 Evaluation Form Yellow form You can leave them in the box by door when you are done.

55 Working together to protect lakes Questions?

56 Hands on Demos!