Certificate of Accreditation

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1 PERRY JOHNSON LABORATORY ACCREDITATION, INC. Certificate of Accreditation Perry Johnson Laboratory Accreditation, Inc. has assessed the Laboratory of: Fish Health and Safety Laboratory, Fisheries Research Centre, (Hereinafter called the Organization) and hereby declares that Organization is accredited in accordance with the recognized International Standard: ISO/IEC 17025:2005 This accreditation demonstrates technical competence for a defined scope and the operation of a laboratory quality management system (as outlined by the joint ISO-ILAC-IAF Communiqué dated April 2017): Biological and Chemical Testing (As detailed in the supplement) Accreditation claims for such testing and/or calibration services shall only be made from addresses referenced within this certificate. This Accreditation is granted subject to the system rules governing the Accreditation referred to above, and the Organization hereby covenants with the Accreditation body s duty to observe and comply with the said rules. For PJLA: Initial Accreditation Date: Issue Date: Expiration Date: Tracy Szerszen President/Operations Manager Perry Johnson Laboratory Accreditation, Inc. (PJLA) 755 W. Big Beaver, Suite 1325 Troy, Michigan November 27, 2015 August 19, 2018 November 30, 2020 Accreditation No: Certificate No.: L The validity of this certificate is maintained through ongoing assessments based on a continuous accreditation cycle. The validity of this certificate should be confirmed through the PJLA website: Page 1 of 6

2 Microbiological F Fish (including Shellfish), Fishery Products, Water and Soil Sampling and Sensory Analysis of Fishes Analysis of Total Plate Count Analysis of Escherichia coli Analysis of Coliforms Analysis of Staphylococcus aureus Analysis of Salmonella Analysis of Vibrio cholerae Analysis of Vibrio parahaemolyticus a) USFDA BAM, 8 th Edition, Revision A,1998 b) GS 655 (SASO 103), Methods of Microbiological Examination of Meat, Fish, Shellfish and their Products c) FSSAI, Manual of Methods of Analysis of Foods, Microbiological Testing d) SASO 48, Methods of Sampling Fish, Shellfish and their Products Detected or Not Detected SASO 1556 (GS 1016), Microbiological Criteria for Food Stuffs Part 1 SASO 708 (GS 380), Chilled Fish SASO 1571 (GS 1361), Chilled Shrimps SASO 2216 Frozen Squid SASO 1570 (GS 1360), Natural Caviar e) SASO 955 (GS 711), Microbiology General guidance for Enumeration of Staphylococcus aureus Colony Count Technique GS 270 (SASO 538), Smoked Fish f) SASO (GS 287E), Microbiology General guidance on Methods for the Detection of Salmonella g) SASO 2023, Microbiology General guidance for the Detection of Vibrio parahaemolyticus h) FAO Issue: 08/2018 This supplement is in conjunction with certificate #L Page 2 of 6

3 Microbiological F Fish, Shellfish and Fish Products Fish, Fish Products, and Shellfish Aeromands (including Aeromonas hydrophila, caviae, sobria and salmonicida) Vibrio spp. Flavobacterium spp. Photobacterium spp. Enterobacteriacae pseudomonas spp. Gram +ve Cocci (Streptococcus iniae ) Isolation and identification of bacterial Pathogens of Aquatic animals using API 20E and 20NE and API for staph and strep. Detection of Aquatic Animal Pathogens by Real-Time PCR Detection of Aquatic Animal Pathogens by PCR 1) AFS Blue Book, 2014: diagnostic Procedures for Finfish and Shellfish Pathogens 2) Austin & Austin, 2012: Bacterial Fish Pathogens, Diseases of farmed and Wild Fish 3) Edward J. Noga, 2010: Fish Diseases, Diagnosis and Treatment (2 nd Ed.) 4) John. G. Holt et al., 1994: Bergey, s Manual of Determinative Bacteriology Kits from Life Technologies Kits from Thermo Fisher Scientific Kits from GenReach Biotechnology Corp. OIE: 2009, 2013, 2014, 2017, 2018 Detected or Not Detected Detected or Not detected Issue: 08/2018 This supplement is in conjunction with certificate #L Page 3 of 6

4 Biological F Fish (including Shell fish), and Fishery Products External Parasitic Infection in Fish Internal Parasitic Infection in Fish (a) Noga, E.J. (2010): Fish Disease and Treatment (2 nd Edn.), Inc. Watsworth Publ.Co., USA, 2 nd Edn. (b) Zale,A.V., D.L.Parris and T.M. Sutton. Fisheries Techniques (3 rd Edn.) Published by American Fisheries Society. Detected /Not Detected [Slight = (+) Moderate = (++) Heavy = (+++)] (c) Heil,N.(Ed) National Wild Fish Health Survey-Laboratory Procedure Manual. (5 th Edn.) U.S. Fish and Wildlife Service, Warm spring GA. Chemical F Plankton (phytoplankton and Zooplankton) Chlorophyll a in and Seawater Seawater, and Soil Extract Count and identification of phytoplankton and zooplankton Extract by acetone and measurement by spectrophotometer Measurement of Nitrogen, Nitrite Diazotization Method (Powder Pillows) in water, wastewater, and seawater using HACH DR/2800 s. (d) Lim, C., C.D. Webster Nutrition: Fish Health. The Haworth Press Inc. NY. Collect the sample by plankton net and examine under the microscope Health HACH Water analysis Handbook N/A N/A 0 mg/l NO2-N to 0.35 mg/l NO2-N Issue: 08/2018 This supplement is in conjunction with certificate #L Page 4 of 6

5 Chemical F Chlorophyll a in Seawater, and Soil Extract Measurement of Nitrogen, Nitrate Cadmium Reduction Method (Powder Pillows) in water, using HACH DR/2800 s Measurement of Sulfate using SulfaVer 4 Method in water, wastewater, and seawater using Hach DR/ 2800 and DR/890 Total and free Chlorine by DPD Method (Powder Pillows) in water, using HACH DR/2800 s Measurement of Cyanide Pyridine-Pyrazalone Method in water, using Hach DR/ 2800 and DR/890 Measurement of Iron, Total FerroVer Method in water, wastewater, and seawater using Hach DR/ 2800 and DR/890 Measurement of Phosphorus, Reactive (Orthophosphate) PhosVer 3 (Ascorbic Acid) Method in water, wastewater, and seawater using HACH DR/ 2800 HACH Water analysis Handbook 0 mg/l NO3-N to 0.5 mg/l NO3-N 2 mg/l SO42- to 70 mg/l SO42-0 mg/l Cl2 to 2 mg/l Cl mg/l CN- to 0.24 mg/l CN mg/l Fe to 3 mg/l Fe 0.02 mg/l PO4 3- to 2.5 mg/l PO4 3- Issue: 08/2018 This supplement is in conjunction with certificate #L Page 5 of 6

6 Chemical F Chlorophyll a in Seawater, and Soil Extract Measurement of Sulfied by Methylene Blue method in water, using HACH DR/ 2800 Measurement of Nitrogen, Ammonia Salicylate Method in water, using HACH DR/ 2800 HACH Water analysis Handbook Soil Analyses Particle size analyses Boyd 1992 N/A Sea Water, Soil, Fish and Shellfish Total mercury (Hg) Heavy metals: Arsenic (As), Copper (Cu), Cadmium (Cd), Lead (Pb), Nickel (Ni), Chromium (Cr), Zinc (Zn), Iron (Fe) Measurement of total mercury (Hg) using direct mercury analyzer (DMA- 80) Manual: Application note : HG/EN-04 HG/EN-09 HG/EN-12 HG/EN-18 Measurement of heavy metals using optical emission spectroscopy with inductively coupled plasma frequency (ICP OES) NFEN ISO November 2009 : determination of element by optical emission spectroscopy with inductively coupled plasma frequency (ICP OES) Manual: Application note : MW-102 MW-114 EPA3051 SK-10 5 µg/l H2S-S to 800 µg/l H2S-S 0.01 mg/l NH3-N to 0.50 mg/l NH3-N 0.05 ng to 600 ng of Hg D.L = 0.01 ng of Hg 0.01 ppm to 1 ppm D.L = ppm 1. The presence of a superscript F means that the laboratory performs testing of the indicated parameter at its fixed location. Example: Outside Micrometer F would mean that the laboratory performs this testing at its fixed location. Issue: 08/2018 This supplement is in conjunction with certificate #L Page 6 of 6