TETON CONSERVATION DISTRICT SAMPLING AND ANALYSIS PLAN FOR THE FLAT CREEK MONITORING PROJECT

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1 TETON CONSERVATION DISTRICT SAMPLING AND ANALYSIS PLAN FOR THE FLAT CREEK MONITORING PROJECT Created: January 2000 Revised: January 2003

2 SAMPLING AND ANALYSIS PLAN Flat Creek/Flat Creek Sub-Watershed/Flat Creek Monitoring Project Created: January 2000 Revised: January 2003 Project Supervisor Randy Williams (307) Phone (307) Fax 230 East Broadway Suite 2A P.O. Box 1070 Jackson, Wyoming Submitted by Brian E. Remlinger Revised January 2003

3 TABLE OF CONTENTS Corrective Actions and Revisions to January Signature Approval Sheets...2,3 Abstract 4 Purpose Statement.5,6 SAMPLING Sampling Personnel and Training....7 Sampling Design.. 8 Sampling Locations and ID Codes.9,10 Proposed Sampling Schedule Parameters and Methods, Chemical ,13 Parameters and Methods, Biological Parameters and Methods, Physical 15 Health and Safety...16 Corrective Actions QUALITY ASSURANCE/ QUALITY CONTROL (QA/QC) Calibration and Maintenance.18 Quality Control Samples 19 Sample Testing/ Contract Laboratories. 20 Field Log Examples..21,22 Revised January 2003

4 DATA Data Archiving...23 Data Review/Validation.24 Lab Results.24 Photographic Documentation.24 Landowners and Contact Information...25 Report Generation.. 26 Statistical Analyses 26 LIST OF TABLES Sampling Personnel.7 Sampling Locations....9,10 Proposed Sampling Schedule.11 Parameters, Chemical...12,13 Parameters, Biological...14 Parameters, Physical..15 Calibration and Maintenance.18 Quality Control Samples 19 Sample Testing/ Contract Labs..20 Data Archiving...23 Landowners and Contact Information...25 Revised January 2003

5 APPENDICES APPENDIX A Credible Data Legislation 1 APPENDIX B - FORMS DEQ-WQD Watershed Program BURP Monitoring Field Data Sheets Chain of Custody Form 1 Equipment Calibration and Maintenance Log (example)....1 APPENDIX C Sample Labeling (example).1 APPENDIX D Fish Creek Watershed Map..1 Sampling Site Location Maps 1 8 APPENDIX E Standard Operating Procedures Table of Contents Included in Appendix Revised January 2003

6 Corrective Actions and Revisions January 2003 Submitted by Brian Remlinger The January 2000 Sampling and Analysis Plan (SAP)- Flat Creek Montioring Project was developed and written by Rik Gay and Heather Alsup. At that time Larry Jorgenson was Chairman of and Steve Kleuss was District Conservationist with the Natural Resource Conservation Service. Revisions to the January 2000 SAP are a result of further monitoring site selection, personnel changes, equipment upgrades, water quality parameter modifications and unknowns discovered. The following are brief descriptions of the revisions to the January 2000 Flat Creek SAP: Pages 2-3 Page 7 Page 9-10 Page 14 Personnel changes to signature approval sheet Sampling personnel changes and training updates Monitoring site updates including addition of Cache Creek and Game Creek monitoring sites Total coliform parameter changed to e. coli bacteria to comply with WDEQ water quality standards Page 18 Multi Probe water analyzer replaced with Hach sension 156 Global Flow Probe upgraded to Marsh-McBirney FLOMATE 2000 Page 19 Page 25 Cache Creek and Game Creek samples added to Quality Control Samples Landowners and Contact Information updated to reflect new monitoring sites Appendix E Sample Site location Maps developed for Cache Creek and Game Creek Revised January

7 Signature Approval Sheet Name Signature Title Organization Randy Williams Executive Director, Project Supervisor Name Signature Title Organization Kate Mead Chairman Board of Directors, Name Signature Title Organization Jenny Castagno District Conservationist Natural Resources Conservation Service Name Signature Title Organization Brian Remlinger Water Resources Specialist, Monitoring Supervisor Name Signature Title Organization Brian Lovett Nonpoint Source Coordinator Wyoming Department of Environmental Quality, Water Quality Division, Watershed Program Revised January

8 Signature Approval Sheet Name Signature Title Organization Mike Foster QA/QC Officer Wyoming Department of Environmental Quality, Water Quality Division, Watershed Program Name Signature Title Organization Bob Wisseman Senior Scientist Aquatic Biology Associates Name Signature Title Organization Ken McMillan Senior Scientist Wyoming Department of Agriculture Laboratory Name Signature Title Organization Ardith Moran Branch Lab Supervisor Department of Health and Welfare Lab Pocatello, Idaho Revised January

9 TETON CONSERVATION DISTRICT SAMPLING AND ANALYSIS PLAN FLAT CREEK MONITORING PROJECT Abstract The headwaters of Flat Creek are located within the Gros Ventre mountain range in the Bridger-Teton National Forest. From there, Flat Creek flows southwest across the National Elk Refuge and through the town of Jackson, all the while being subjected to various impacts. These impacts range from large amounts of fecal contaminants due to the wildlife habitat of the National Elk Refuge to increasing urban impacts such as road construction, sewer, and urban storm run-off. Flat Creek runs through a campground, residential, industrial and agricultural areas, and receives water from two separate diversions before its confluence with the Snake River. Residents of Teton County have voiced concern about the questionable health of Flat Creek. This water body was also listed on the Department of Environmental Quality s 303d list of possibly impaired waterbodies. Because of these concerns, the is implementing a water quality monitoring project on Flat Creek in order to assess the quality of this water body. The has utilized the public input process by inviting landowners along Flat Creek and other concerned citizens to attend meetings in which this project has been designed. Citizens feel that although the condition of Flat Creek has improved in many respects over the years, there are still some perceptions of poor ecological integrity due to water contamination resulting from increasing pressure on the system including population growth, increased urban development, and non-point source pollutants. It is the intention of the to assess the overall health and water quality of Flat Creek, and also act as a guard against future negative impacts from occurring on this stream in the future. This SAP is intended as a guide for personnel who will be conducting the water quality monitoring activities for the Flat Creek Monitoring Project, as a QA/QC plan, and as a data management plan. Samples are collected using the methods, procedures and/or protocols in the Natural Resources Conservation Service National Handbook of Water Quality Monitoring, May 1998, and the Wyoming Department of Environmental Quality, Water Quality Division, Watershed Program, Manual For Standard Operating Procedures for Sample Collection and Analysis, A copy of the entire SOP is on file at the Teton Conservation District and all relevant sections of the SOP are contained in the Flat Creek Sampling and Analysis Plan. Revised January

10 Purpose Statement The has responded to public concern of possible water quality issues affecting Flat Creek by working with landowners along the creek, as well as others interested in the study, to implement a water quality-monitoring project. Specific water quality concerns pertaining to Flat Creek include increased urban impacts such as storm water run-off, septic systems near Flat Creek, garbage and solid waste. There are also concerns of future growth of the town of Jackson and the possible threats this poses to the health of the stream system. Non-point source pollution such as fertilizers and herbicides, as well as the state of the fisheries are concerns as well. Without physical, chemical, and biological data pertaining to Flat Creek, it is difficult to substantiate concerns of these and future human impacts on water quality, habitat, and general health. There are several purposes to this study. The ultimate purpose for implementing this monitoring project is to establish baseline data where none exists and to establish trends in the water quality of Flat Creek. There were also twelve objectives set forth by the stream committee as guidelines for measuring project success. They are as follows: 1) To improve the condition of Flat Creek, as well as the entire watershed. 2) To collect credible data pertaining to Flat Creek in order to validate concerns. 3) To increase landowner, as well as general public awareness, education, and participation on issues regarding Flat Creek. 4) To work in conjunction with town and county planners and gain political strength to take on current problems and prevent negative impacts from occurring in the future. 5) To research ordinances concerning development, define legal jurisdiction against negative impacts, and develop stream laws pertaining to Flat Creek. 6) To assess the number of septic systems along Flat Creek and convert them to the town sewer system. 7) To improve diversions and irrigation management regarding Flat Creek. 8) To assess the amount of private land in the Flat Creek watershed that can be developed. 9) To collect historical data relevant to Flat Creek. 10) To secure funding for a watershed protection and enhancement program. 11) To improve fisheries habitat where it needs to be done. 12) To use the public school system and the Teton Science School as a resource for monitoring water quality and also as a means of publicizing efforts to maintain high water quality within the Flat Creek watershed. Some of these objectives do not deal directly with water quality issues and though they may not be mentioned in this plan, it is the intention of the Teton Conservation District to meet each of them. Through this study, the hopes to better understand the causal effect of human impact on the natural system of Flat Revised January

11 Creek. Our monitoring efforts will answer questions concerned people have been asking for a number of years. employees and field samplers will follow the guidelines set forth in this sampling and analysis plan, as well as those set forth in the Wyoming Department of Environmental Quality, Water Quality Division, Watershed Program, Manual of Standard Operating Procedures for Sample Collection and Analysis, August Revised January

12 Organization, Stakeholder, or Group Name Sampling Personnel Number Of Personnel Type of Training 3 NRCS Water Quality Monitoring Training and WACD Water Quality Training, Fluvial Geomorphology Training Natural Resources Conservation Service 1 NRCS Water Water Quality Monitoring Training Private Landowners Varies Landowners who contribute will be trained by TCD staff in staff gauge reading, maintaining field log books, and become familiar with field observation procedures as outlined in the WDEQ SOP manual. Date of Last Training Amount of Previous Sampling Experience field seasons 5 projects field seasons 150 projects 2000 None Revised January

13 Sampling Design One of the goals of the Flat Creek water quality-monitoring project is to establish trends depicting the quality of the water of Flat Creek. Trends are considered persistent changes in water quality variables over time. Because of the lack of adequate historical data pertaining to Flat Creek, it will be important to establish a baseline and then monitor the changes, or trends that occur. Trend stations are ideal to monitor changes over time. When establishing trend stations on a water body, it is crucial to use a control station where land use is stable and impact is minimal. There should not be changes in land use practices during the life of the water quality investigation at the control site. The Teton Conservation District has chosen a control site within the Bridger-Teton National Forest boundaries east of the National Elk Refuge. It is an area near the headwaters of Flat Creek that receives little human disturbance with the exception of some recreation that takes place in the vicinity of the control site. Since the US Forest Service manages this area, the land use should not change within the course of the study. The other sites have been chosen to best represent Flat Creek and all possible disturbance areas. The chemical, physical, and biological data collected will be used to draw conclusions as to the overall condition of Flat Creek. The length of the study will depend on the findings, although it is expected to last a minimum of ten years. Following this study, it is the desire of to randomly monitor Flat Creek in order to maintain an ongoing understanding of the water body. As trends are established, monitoring schedules may vary depending on ongoing results. The National Elk Refuge has asked the to add two additional sites to the monitoring project to better assess wildlife influences in the area. National Elk Refuge personnel will work closely with the in monitoring these two sites which will be located on Nowlin Creek, a tributary of Flat Creek. Their exact locations will be chosen in the spring of 2000 and will be added to this Sampling and Analysis Plan in the Corrective Actions section. A site will also be founded on Cache Creek, another major tributary of Flat Creek that may be directly impacted by urban influence. Revised January

14 Sample Site ID Code FLC FL1 FL2 FL3 FL4 FL5 NC1 CC1 CC2 Latitude/Longitude Coordinates from GPS (1/4, section, township, range, UTM coordinates) 43 33, N , W SW ¼ SE ¼ Sec 35 T 42N R 115W 43 33, N ,75.00 W NW ¼ NE ¼ Sec 11 T 41N R 116W 43 29, N , W SE ¼ NE ¼ Sec 28 N 41N R 116W N W NW ¼ NE ¼ Sec33 T 41N R 116W N W SE ¼ NE ¼ Sec 06 T 41N R 116W N W NW ¼ SW ¼ Sec 28 T 40N R 116W N W SE ¼ NW ¼ Sec 13 T 14N R 116W N W N W Sampling Locations HUC Code for Sample Site Type of Land Ownership Public USFS (site determined by committee) Public FWS (site determined by TCD and USFW-NER) Private (site determined by committee) Private (site determined by committee) Private (site determined by committee) Public-WY Game & Fish (site determined by committee) Public NER Management (site determined by TCD and Sample Site Location Narrative Flat Creek Control: Flat Creek on Bridger-Teton National Forest. Follow Elk Refuge road from East Jackson to Forest Service bndry at Flat Creek USFS sign make right down steep road to site Park at National Fish Hatchery. Walk out road to NE on Elk Refuge. After 200 yards head down to Flat Creek and look for Stake along west bank of Creek. Just below Saddle Butte Bridge on Flat Creek In Jackson, drive to the end of Hansen Ave. at Flat Creek. Walk down to creek and site is below Cache Creek outlet In Jackson, just north of High School Road Bridge on Flat Creek. Drive on South Park Elk Feed Gounds access road from Highway 89 west to bridge over Flat Creek. Site is 50 yards upstream. From East Jackson, drive onto Elk Refuge road. Take left to Nowlin Creek road on north side of Miller Butte. After third pond walk down to creek. Site is 50 yards past pond outlet. USFW-NER) Public-USFS Drive to Cache Creek Trailhead and walk 1.8 miles up forest road. Walk down to Cache Creek and look for green USGS gauging staion Public-USFS Drive to Cache Creek trailhead. Walk down the creek until reaching foot bridge. Sample spot is just below bridge. Revised January

15 CC3 GC1 GC2 GC3 GC N W NW ¼ NE ¼ Sec33 T 41N R 116W N W N W N W N W Private In Jackson, drive to the end of Hansen Ave. at Flat Creek. Walk down to creek and site is at Cache Creek outlet where large concrete culvert exits ground Public-USFS Park at Game Creek trailhead and walk 3.3 miles up Forest Service Road to a crossing of the north fork Game Creek. Take sample just above crossing Public-USFS Park at the Game Creek trailhead and walk 1/3 of a mile to the first crossing of Game Creek. The sample site is just above the culvert Public-USFS Park at the Game Creek trailhead. Walk below Game Creek Road where creek exits culvert. Take sample 30 feet below culvert Public - USFS Drive 0.8 miles up Game Creek Road from Highway 89. Walk directly south to Game Creek. Revised January

16 Proposed Sampling Schedule (indicate whether it is periodic sampling or event driven sampling) Sampling Category (all sites receive same sampling) Biological Physical Proposed Sampling Dates for Periodic Sampling Calendar yearly sampling, of macros and fecal coliform beginning in late Fall of 2000 and continuing for the next 5-10 years, dependent upon ongoing results. Three sites will be selected for electro-shocking in September by Wyoming Game and Fish and work will be done two days per year in September. One time annually in late Fall, dependent upon stream flow level. Event(s) Parameter(s) to be sampled Macroinvertebrates, Electro-shocking, Redd counts, Fecal Coliform sampling Discharge, Embeddedness, Habitat Assessment, Pool/riffle ratio, Substrate Chemical Specific chemical parameters including ph, DO, Temperature, and Conductivity measured during physical sampling which will take place annually in late fall. Full-scale chemical sampling will take place twice each year, dependent upon prerunoff and post-runoff times. Chloride, Fluoride, Nitrate, ph, Total dissolved solids, Total Nitrogen, Total Phosphorous, Total Suspended Solids, Turbidity Revised January

17 Parameters and Sample Collection Methods Parameter Reporting Units Test Method Number or Name Chemical Parameters Preservative Holding Time Biochemical Oxygen Demand (BOD) Dissolved Oxygen (DO) mg/l mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method Ice USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method 360.2, USEPA 1983 Probe: None 48 Hours Must be measured on site Chloride mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method None required 28 Days Fluoride mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method None required 28 Days Phosphorous, Total mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method 365.3, colorimetric ph Units USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method Sulfuric Acid 28 Days None Must be measured on site Revised January

18 Parameter Reporting Units Test Method Number or Name Temperature N/A USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method Preservative None Holding Time Must be measured on site Total Dissolved Solids (TDS) mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method Ice 7 Days Total Nitrogen (Kjeldahl) mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Sulfuric Acid 28 Days Total Suspended Solids (TSS) mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method Ice 7 Days Turbidity NTU USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / , Method None Must be measured in field Revised January

19 Biological Parameters Parameter e. coli bacteria with result reported as CFU s (colony forming units) per 100 ml Reporting Units CFU s Test Method Number or Name SOP for e. coli bacteria sampling, DEQ-WQD SOP Manual, August 1999 adapted from USEPA, Microbiological Methods for Monitoring the Environment, Water and Wastes, EPA-600 / , December 1978, p , p.59-90, incorporated by ref. Preservative Ice immediately. Samples must be kept at or below 4 C. Holding Time 6 Hours Office filtration and incubation, HACH EPA Approved Method 10029, Coliforms: Membrane Filtration (simultaneous detection), also see Teton Conservation Distric s Sampling and Analysis Plan for Bacterial Density Analysis, January 2003 Macroinvertebrates N/A SOP for Macroinvertebrate sampling and for macroinvertebrate sample preservative, DEQ-WQD SOP Manual, August 1999 Formalin; see SOP Once sample is preserved, keep out of direct sun and away from direct exposure to heat. Electro-shocking and redd counts (contracted out to WY Game & Fish) N/A SOP for electro-shocking and redd counts as guided by Wyoming Game & Fish. Contact John Keifling at Wyoming Game and Fish (Jackson Branch) for further information. None Must be measured in field. Revised January

20 Parameter Reporting Units Physical Parameters Test Method Number or Name Bank Stability N/A Refer to SOP Manual, WDEQ, Water Quality Division Bankfull Width N/A Refer to SOP Manual, WDEQ, Water Quality Division Riffle Gradient N/A Refer to SOP Manual, WDEQ, Water Quality Division BURP Monitoring Strahler Stream Order Method N/A Refer to SOP Manual, WDEQ, Water Quality Division Stream Discharge N/A Refer to SOP Manual, WDEQ, Water Quality Division Embeddedness N/A Refer to SOP Manual, WDEQ, Water Quality Division Global Positioning System Data (GPS) Habitat Assessment High Gradient Streams Habitat Assessment Low Gradient Streams N/A N/A N/A Refer to SOP Manual, WDEQ, Water Quality Division Refer to SOP Manual, WDEQ, Water Quality Division Refer to SOP Manual, WDEQ, Water Quality Division Pool Quality N/A Refer to SOP Manual, WDEQ, Water Quality Division Width to Depth Ratio N/A Refer to SOP Manual, WDEQ, Water Quality Division Preservative N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Holding Time Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Must be measured in the field. Revised January

21 Health and Safety The will take the maximum precautions regarding health and safety into the Flat Creek monitoring project. Field samplers will have first aid kits with them at all times and will be trained in basic first aid and adult CPR. All field samplers will have knowledge of the surrounding hospitals and emergency medical clinic locations. Field samplers will always sample with at least two personnel to assure safety. Sampling personnel will always follow health and safety measures outlined in the DEQ-SOP, August EMERGENCY PHONE NUMBERS AND ADDRESSES: ST. JOHNS HOSPITAL EMERG+A+CARE OF JACKSON HOLE EMERGENCY DIAL 911 EMERGENCY DIAL 911 OR CALL (307) OR CALL (307) EAST BROADWAY POWDERHORN MALL 974 WEST BROADWAY JACKSON, WY JACKSON, WY TRAINING FOR FIRST AID AND CPR ST.JOHN S HOSPITAL CALL FOR CLASS INFORMATION OR TETON COUNTY PARKS AND RECREATION Revised January

22 Corrective Actions Any need for modification in the existing monitoring plan including; site locations, number of samples per site, number or type of quality control samples, sampling methods or standard operating procedures, number of sites, or database application will be decided concurrently by the project manager, the stream committee, and field samplers. If any change is warranted, the project manager will insert the dated amendment in to the Sampling and Analysis Plan and send the amendment to the Department of Environmental Quality to insert in their copy. The project manager will also notify all field samplers of amendments in the SAP. The project manager on an as needed basis will assess the effects of changes in the monitoring plan incurred. In the event that any corrective action needs to be implemented, employees and field samplers will follow procedures outlined in the WDEQ SOP Manual, August Revised January

23 Calibration and Maintenance of Equipment Item Log(s) Calibration Maintenance SOP Hach sension156 MultiProbe ph, DO and Conductivity Meter Surber Surber Sampler Magellan GPS ColorTRAK Satellite Navigator GPS (Global Positioning System) Ben Meadows DH-48 Integrated water sampler Marsh- Mcbirney FLO-MATE Model 2000 w/wading rod Calibration and Maintenance log kept on file at the Teton Conservation District. Maintenance log kept on file at the Teton Conservation District. Calibration log kept on file at the Teton Conservation District. Maintenance log kept on file at the Teton Conservation District. Calibration and Maintenance log kept on file at the Teton Conservation District. Once a day. ph 7 and 10 buffer standards are supplied by the conservation district; DO probe is air calib. None required Time set as needed (system does not account for daylight savings changes) No calibration necessary. No calibration necessary. No field maintenance except electrode replacement; meters are returned to the conservation district for repair Check net before each use for tears and holes in the netting; remove any leftover debris No field maintenance; GPS is returned to the conservation district and sent to the manufacturer for repair No field maintenance; DH-48 is returned to the conservation district and sent to the manufacturer for repair. No field maintenance; FLO-MATE is returned to the conservation district and sent to the manufacturer for repair. Collect water samples which best represent the entire water body. For complete detail, refer to WDEQ SOP Manual. Stand downstream from site and collect macroinvertebrate sample. For complete detail, refer to WDEQ SOP manual. Use system to calculate specific location. For complete detail, refer to WDEQ SOP manual Use system to integrate water samples. For complete detail, refer to WDEQ SOP manual. Use system to measure flow and velocity in streams and rivers. For complete detail, refer to WDEQ SOP manual. Revised January

24 Quality Control Samples Sample Site ID Code FLC FL1 FL2 Type of QC Sample Duplicates and Field Blanks Duplicates and Field Blanks Duplicates and Field Blanks Number or % of QC Samples 1 duplicate every five sites, and one field blank per sampling event 1 duplicate every five sites, and one field blank per sampling event 1 duplicate every five sites, and one field blank per sampling event QC Sample Schedule Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. FL3 FL4 Duplicates and Field Blanks Duplicates and Field Blanks 1 duplicate every five sites, and one field blank per sampling event 1 duplicate every fifth sites, and one field blank per sampling event Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. FL5 Duplicates and Field Blanks 1 duplicate every fifth sites, and one field blank per sampling event Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. NC1 Duplicates and Field Blanks 1 duplicate every fifth sites, and one field blank per sampling event Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. CC1, CC2, CC3 Duplicates and Field Blanks 1 duplicate every fifth sites, and one field blank per sampling event Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. GC1, GC2, GC3, GC4 Duplicates and Field Blanks 1 duplicate every fifth sites, and one field blank per sampling event Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler. Revised January

25 Sample Testing/ Contract Labs Laboratory Address Contact Tests Aquatic Biology Associates Department of Health and Welfare Laboratory Wyoming Department of Agriculture Analytical Services 3490 NW Deer Run Road Corvallis, OR Memorial Drive Pocatello, ID Snowy Range Road Laramie, WY Wyoming Game and Fish P.O. Box 67 Jackson, WY Bob Wisseman Senior Scientist (541) Ardith Moran Branch Lab Supervisor (208) Ken McMillian Senior Scientist (307) Ralph Hudelson Fisheries Biologist (307) Macroinvertebr ate Sample Count and Identification Fecal Coliform and E. Coli Identification Surface Water Collection and Analysis Electroshocking and redd counts Macroinvertebrate and surface water samples will be delivered by way of courier. Surface water samples for chemical analysis must arrive at the lab to be analyzed within 48 hours. The samplers must mail surface water samples on the same day they are collected to ensure their timely arrival. Macroinvertebrate samples must be kept in an alcohol preservative (see WDEQ SOP 1999) and out of direct heat and sunlight until they are shipped to the lab. Macroinvertebrate samples will be sent via United Parcel Service (UPS) and surface water samples will be sent via Federal Express. It is the samplers responsibility to pack the water samples and take them to Federal Express before 5:00PM Monday through Thursday to be shipped to the lab. Samplers should avoid sampling for surface water on Friday, as samples will not be analyzed within 48 hours due to the weekend. Fecal Coliform and E. Coli counts have a holding time of only six hours and therefore need to be delivered to the laboratory in Pocatello, Idaho on the same day as sampling takes place. Samplers should arrive at the lab by 2:30PM as instructed by the lab. Samplers will need to start sampling early enough to accommodate the arrival of samples to the lab by the appropriate time. Electro-shocking and redd counts will be performed by Wyoming Game and Fish and the will assist in the sampling efforts each September. Wyoming Game and Fish will follow proper procedure as detailed in Wyoming Game and Fish sampling protocol. Sampling results will be given to the Teton Conservation District in the form or paper or electronic results and incorporated into the Flat Creek Monitoring Project files. Revised January

26 Field Log Books When using field log books, samplers will follow standard operating procedures outlined for field log books as detailed in the WDEQ SOP Manual August, The front cover will appear as follows: TETON CONSERVATION DISTRICT FIELD LOG BOOK SAMPLERS NAME (PRINTED): DATE FROM: mm/dd/yy - / / DATE TO: mm/dd/yy - / / SEQUENTIAL LOG BOOK NUMBER: The inside cover will appear as follows: TETON CONSERVATION DISTRICT FIELD LOG BOOK (ANY PERSON WHO MAKES AN ENTRY IN THIS LOG BOOK MUST SIGN HERE) SAMPLER SIGNATURE DATE INITIALS Revised January

27 Field Log Books The entry pages will appear as follows: TETON CONSERVATION DISTRICT FIELD LOG BOOK OBSERVER: DATE AND TIME: DISCHARGE RATING: CURRENT WEATHER CONDITIONS: WEATHER CONDITIONS (IN PREVIOUS 12 HOURS): RECENT PRECIPITATION (IF SO, HOW MUCH?: STAFF GUAGE READING: FIELD OBSERVATIONS: Revised January

28 Data Archiving Data Item Location Retention Time Chain of Custody forms Equipment Calibration Logs Field Data Sheets Field Log Books Laboratory Test Results (original raw data) Database/Spreadsheets Statistical Analyses Final Report Photographs office office office office office office office office office 3 Years 3 Years Permanently Permanently Permanently Permanently Permanently Permanently Permanently When data archiving, employees and field samplers will always follow procedures outlined in WDEQ SOP Manual, August Revised January

29 Data Review and Validation All data collected and reported will receive a 100 percent error check. The monitoring supervisor and the Natural Resources Conservation Service District Conservationist will be responsible for all data review and validation. Both will use methods and correction procedures as outlined in the USDA-NRCS National Handbook of Water Quality Monitoring for accuracy in all review and validation. Laboratory Results All laboratory results will be stored in paper and electronic format. Direct results sent from laboratories will be kept in a file at the. Results will also be entered into electronic database format for analyses. A copy of all electronic database entries will be kept in the form of zip disc and stored off of Teton Conservation District property in case damage to property occurs. Paper report will be put into electronic file by the project supervisor, or will be delegated to other staff and supervised by the project supervisor. Laboratory results will be analyzed and used to evaluate the condition of Flat Creek and program effectiveness. Photographic Documentation Photographic documentation will start with the BURP/BIOASSESSMENT field data sheet in which the field sampler will fill in the appropriate blanks pertaining to taking photographs. Field sampler will indicate the frame number, date, and time of the upstream, downstream, and panorama photographs taken. Field samplers will take one photograph upstream and one downstream. Panorama photos will consist of one frame taken in all directions including North, Northeast, East, Southeast, South, Southwest, West, and Northwest, which will account for a 360 view of the site. All captions should include frame number, date and time, subject, location in narrative, and latitude/ longitude coordinates (which are included on the first page of the field data sheets. At the end of each sampling event or day, all photographs taken will be downloaded into the computer at the and cataloged. A copy of all photographs taken will be kept on zip disc off property in case damage to conservation district property occurs. The uses a FUJIFILM MX-700 digital camera. The camera is kept at the and instructions are kept with the camera in its case. Field samplers will always follow standard operating procedures for photographic documentation as outlined in the WDEQ SOP Manual, August Revised January

30 Landowners and Contact Information The Flat Creek Monitoring Project is a collaborative effort between the Teton Conservation District and local landowners that have expressed interest in the project. The control site (FLC) is located on Bridger-Teton National Forest land. Sites two through four, (FL2-FL4) are located on private property. Site five (FL5) is located on the Wyoming Game and Fish Big Game Refuge. Sites seven (NC1) is located on Nowlin Creek on the National Elk Refuge. Cache Creek sites one and two (CC1 and CC2) are located on Bridger-Teton National Forest and site three (CC3) is located on private property. All Game Creek sites are located on Bridger-Teton National Forest. The Teton Conservation District has the permission from the US Forest Service, the National Elk Refuge, Wyoming Game and Fish, the National Fish Hatchery, and all other landowners to monitor as necessary on their property and will always notify landowners at least one day in advance of monitoring efforts. The following list represents landowners and contact information regarding the monitoring sites: Contact Site Telephone Number Address US Forest Service Contact Nancy Hall FLC (307) P.O. Box 1888 Jackson, WY National Fish Hatchery Contact Kerry Grande FL1 (307) Fish Hatchery Jackson, WY Triano and Copp FL2 (307) P.O. Box Jackson, WY Pete Karns FL3 (307) P.O. Box 40 Jackson, WY Mike Martin FL4 (307) P.O. Box 1743 Jackson, WY Wyoming Game and Fish Contact Steve Kilpatrick FL5 (307) P.O. Box 67 Jackson, WY National Elk Refuge Contact Eric Cole NC1 (307) P.O. Box C Jackson, WY US Forest Service Contact Nancy Hall CC1, CC2, GC1, GC2, (307) P.O. Box 1888 Jackson, WY GC3 and GC4 Pete Karns CC3 (307) P.O. Box 40 Jackson, WY Revised January

31 Report Generation All paper reports will be kept in the Flat Creek Monitoring project files at the. Paper reports including all reports sent to the conservation district by contract labs will be kept on file at the district office. Information collected will be stored in electronic format as well and reports generated from information collected will be in electronic form. The district will produce reports on the findings of the monitoring project and will use GIS for the generation of these reports. Field samplers will always comply with procedures outlined in WDEQ SOP Manual, August 1999 when generating reports. Statistical Analyses The has taken measures to ensure that the number of sample sites is adequate as well as ensuring that the correct number of samples are being taken in order to reduce the amount of statistical variation. The district has also taken adequate quality control measures to assure that the findings of this project are accurate and best represent the water quality of Flat Creek. The format for analyses is yet to be determined by the Teton Conservation District, which is awaiting Phase V training of Water Quality Monitoring to be sponsored by The Department of Environmental Quality, Wyoming Department of Agriculture, and the Wyoming Association of Conservation Districts. Upon completion of this training, the will develop a database management system that complies with policies set forth within the Phase V training session. Revised January

32 Manual of Standard Operating Procedures for Sample Collection and Analysis TABLE OF CONTENTS (NOTE: This table of contents only contains subjects relative to the s Flat Creek Monitoring Project. The entire SOP is on file at The.) PART 1 SAMPLE PLANNING AND SITE LOCATIONS Below Only Monitoring.1 2 BURP Monitoring Field Data Sheet Review.1 2 BURP Monitoring Preliminary Data Review 1 2 BURP Monitoring Procedure Sequence.1 2 BURP Pre-Monitoring Site Evaluation..1 2 BURP Reference Site Locations (including bioassessment reference sites) BURP Reference Site Scheduled Monitoring 1 2 Non-point Source Pollution Bioassessments..1 2 Sampling Locations, Determining..1 2 PART 2 BIOLOGICAL SAMPLING Fecal Coliform Sampling 1-4 Fecal Coliform Testing (including Bacterial Sample Analysis Log).1 8 Geometric Mean, Calculating and Using (for fecal coliform test results)..1 4 Macroinvertebrate Sample Identification (including Subsampling Form).1 8 Macroinvertebrate Sample Preservative Macroinvertebrate Sampling..1 6 Revised January 2003

33 PART 3 PHYSICAL SAMPLING Bank Stability, Evaluating. 1 2 Bankfull Width, Determining 1 2 Bioassessment Riffle Gradient BURP Monitoring Strahler Stream Order Method Current Velocity, Measuring for BURP Monitoring.1 2 Discharge, Stream..1 4 Embeddedness (silt covering) Rating 1 2 Geology Abbreviations, Surficial..1 6 Global Positioning System (GPS) Data (including GPS Locational Data Sheet).1 4 Habitat Assessment, High Gradient Streams.1 4 Habitat Assessment, Low Gradient Streams..1 4 Per Cent Fines 1 2 Pool Quality Soil Type Abbreviations and Definitions..1 4 Width to Depth Ratio.1 2 PART 4 CHEMICAL SAMPLING Biochemical Oxygen Demand (BOD), 5 day 20 C..1 2 Chloride..1 2 Dissolved Oxygen (DO) 1 2 Fluoride..1 2 Revised January 2003

34 Hardness, Total (as Calcium Carbonate) Nitrogen, Total Kjeldahl 1 2 ph Temperature, Water Total Dissolved Solids (TDS) 1 2 Total Suspended Solids (TSS) Turbidity 1 2 PART 5 QUALITY CONTROL, CUSTODY AND REPORTING Abbreviations.1 4 Bioassessment Quality Control Criteria.1 2 Blanks BURP Monitoring Core Parameters..1 2 BURP Monitoring Electronic Data Entry..1 2 Chain of Custody (including three types of Chain of Custody Forms).1 8 Cleaning Previously Used Sampling Bottles.1 2 Conversion Factors 1 6 Data Archiving (including sample Transfer of Records and Box Label Forms) Duplicates..1 2 Ecoregion Definition..1 2 Field Log Books.1 2 Holding Time.1 2 Instrument Calibration and Calibration Logs 1 2 Macroinvertebrate Reference Collection Revised January 2003

35 Material Safety Data Sheets (MSDS) 1 2 Photographic Documentation 1 8 Quality Control Measures, Summary of Safety and Safety Equipment 1 2 Sample Collection..1 2 Sample Labeling 1 2 Sample Preservation..1 4 Split Samples.1 2 Surber Sample Techniques 1 2 Revised January 2003

36 APPENDIX A CREDIBLE DATA LEGISLATION Revised January 2003

37 APPENDIX B FORMS 1. DEQ-WQD Watershed Program BURP Monitoring Field Data Sheets 2. Chain of Custody Form 3. Equipment Calibration and Maintenance Log (example) Revised January 2003

38 APPENDIX C Sample Labeling (example) Revised January 2003

39 APPENDIX D Sampling Site Location Maps Revised January 2003

40 APPENDIX E References and Information Resources Revised January 2003

41 APPENDIX F Standard Operating Procedures o Table Of Contents Included Revised January 2003