Methods of microbiological examination for air in public places - Determination of aerobic bacterial count

Transcription

1 Translated English of Chinese Standard: GB/T Translated by: Wayne Zheng et al. NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB GB/T Methods of microbiological examination for air in public places - Determination of aerobic bacterial count 公共场所空气微生物检验方法细菌总数测定 Issued on: September 30, 2000 Implemented on: January 01, 2001 Issued by: National Quality and Technical Supervision Bureau Page 1 of 9

2 Tips - GB Series (not part of this Standard) Standard GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T GB/T Description Methods of microbiological examination for air in public places - Determination of aerobic bacterial count Methods of microbiological examination for tea set in public places - Determination of aerobic bacterial count Methods of microbiological examination for tea set in public places - Determination of coliform bacteria Methods of microbiological examination for towel and bedclothes in public places - Determination of aerobic bacterial count Methods of microbiological examination for towel and bedclothes in public places - Determination of Coliform bacteria Methods of microbiological examination for barber's tools - Determination of Coliform bacteria Methods of microbiological examination for barber's tools - Determination of Staphylococcus aureus Methods of microbiological examination for slippers in public places - Determination of molds and yeasts count Methods of microbiological examination for water in swimming pool - Determination of aerobic bacterial count Methods of microbiological examination for water in swimming pool - Determination of Coliform bacteria Methods of microbiological examination for bath tube and wash basin in public places - Determination of aerobic bacterial count Methods of microbiological examination for bath tube and wash basin in public places - Determination of Coliform bacteria Issued Date Enforced Date Newer Version (Click to Check) GB/T Methods for determination of air temperature in public places Page 2 of 9

3 GB/T Methods for determination of air humidity in public places GB/T Methods for determination of wind speed in public places GB/T Method for determination of atmospheric pressure in public places GB/T Methods for determination of thermal radiation in public places GB/T GB/T Method for determination of air change flow of indoor air in public places Method for measurement of infiltration rates of indoor air in public places GB/T Methods for determination of daylighting efficient in public places GB/T Method for determination of illumination in public places GB/T Method for determination of noise in public places GB/T Methods for determination of carbon monoxide in air of public places GB/T Methods for determination of carbon dioxide in air of public places GB/T Method for determination of ammonia in air of public places GB/T Methods for determination of formaldehyde in air of public places GB/T Method of examination of ozone in air of public places GB/T Method for determination of temperature in water of swimming place GB/T Method for determination of urea in water of swimming place GB/T GB/T GB/T Hygiene inspection methods in public places - Part 1: Physical factors Hygiene inspection methods in public places - Part 3: Airborne microbes Hygiene inspection methods in public places - Part 4: Public appliances microorganisms Page 3 of 9

4 GB/T GB/T Hygiene inspection methods in public places - Part 5: Air conditioning system Hygiene inspection methods in public places - Part 6: Health monitoring technical specifications Page 4 of 9

5 Table of Contents Foreword Scope Definitions Instruments and devices Medium Operational steps Page 5 of 9

6 Foreword In order to implement the "Public Places Hygienic Management Regulations" and GB 9663~ , GB "Public Places Hygienic Standard, and to strengthen the hygienic supervision and management of public places, this Standard is formulated. The methods in this Standard are the supervision and inspection methods supporting to GB 9663~ The first method of this Standard is the arbitration method. This Standard is released for the first-time. This Standard is proposed by Ministry of Health, the People's Republic of China. Drafting organizations of this Standard: Chinese Academy of Preventive Medicine and Environmental Hygiene Monitoring Institute, Beijing Municipal Health and Epidemic Prevention Station, Guangdong Provincial Health and Epidemic Prevention Station, and Tianjing Municipal Health and Epidemic Prevention Station. The main drafters of this Standard: Zhou Shuyu, Chen Xiping, Gao Hui, Cai Han, and Zhang Shulan. Page 6 of 9

7 Methods of microbiological examination for air in public places - Determination of aerobic bacterial count 1 Scope This Standard specifies the determination method of total number of bacteria in the air of public places. This Standard applies to the determination of total number of bacteria in the air of public places. 2 Definitions This Standard uses the following definitions. 2.1 Impacting method It refers to the sampling determination method - USE percussive air microbial sampler to sample; through pumping dynamic action, LET air go through slits or small holes so as to generate high-speed airflow; so that the bacteria-carrying particles suspended in air hit onto nutrient agar plate; after incubating at 37ºC for 48 h; CALCULATE the number of bacterial colonies contained in each cubic meter of air. 2.2 Natural sinking method It refers to the sampling determination method - A nutrient agar plate, diameter of 9 cm, is exposed for 5 min at the sampling point; after incubating at 37ºC for 48 h; CALCULATE the number of grown bacterial colonies. 3 Instruments and devices 3.1 High-pressure steam sterilizer. 3.2 Dry-heat Sterilizer. 3.3 Thermostat incubator. 3.4 Refrigerator. 3.5 Petri dish (diameter of 9 cm). 3.6 General devices for preparing medium: Measuring bottle, conical flask, ph meter or precision ph paper, etc. 3.7 Percussive air microbial sampler. Page 7 of 9

8 and shall avoid those air-circulation locations such as air conditioning, windows and doors PLACE the nutrient agar plate at the sampling point, OPEN the dish lid, EXPOSE for 5min, COVER the dish lid, FLIP the plate, PLACE into 36ºC ± 1ºC thermostat incubator to INCUBATE for 48 h COUNT the number of colonies grown on each plate, CALCULATE the average number of colonies of all sampling points. The number of colonies per petri dish (cfu/dish) shall be reported as the result. Page 9 of 9