IOWA STATE UNIVERSITY 2011 IOWA EVALUATION OF INSECTICIDES AND PLANT-INCORPORATED PROTECTANTS

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1 IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY 20 IOWA EVALUATION OF INSECTICIDES AND PLANT-INCORPORATED PROTECTANTS CORN PESTS RESEARCH PROJECT DEPARTMENT OF ENTOMOLOGY AMES, IOWA SUE BLODGETT, CHAIR INSECTS INVESTIGATED Corn Rootworm European corn borer Corn Earworm Report Authors April 0, 202 Aaron Gassmann File number: 289- Patrick Weber

2 20 IOWA EVALUATION OF INSECTICIDES AND PLANT INCORPORATED PROTECTANTS TABLE OF CONTENTS Page. Research Personnel Introduction and Objective Testing Procedures and Evaluations Studies Conducted Corn Rootworm Evaluation Tables Ames Pioneer CMRA study Root Injury/Percent Consistency... Stand Count... Yield... Pioneer dual pyramid efficacy and yield for corn rootworm Stand Count... Root Injury/Percent Consistency... 6 Yield... 6 Monsanto corn rootworm study Root Injury/Percent Consistency... 7 Stand Count... 7 Yield... 8 Monsanto genuity VT3P seed blend study Stand Count... 8 Yield... 9

3 Table of Contents (Continued) Page Ames Monsanto smartstax features and benefits study Stand Count... 9 Yield Ames (Old Dairy Farm) Syngenta experimental hybrid efficacy study Root Injury/Percent Consistency Ames (South Dairy Farm) Syngenta experimental hybrid efficacy study Root Injury/Percent Consistency... 2 Crawfordsville Yield study Root Injury/Percent Consistency... 2 Stand Count Yield Monsanto smartstax refuge in bag study Stand Count Yield Monsanto smartstax features and benefits study Stand Count... 2 Yield... 2 Northeast Iowa Monsanto corn rootworm study Root Injury/Percent Consistency... 2 Stand Count... 2 Percent Lodging Yield Nashua Yield study Root Injury/Percent Consistency Stand Count Percent Lodging Yield... 28

4 Table of Contents (Continued) Page Monsanto corn rootworm study Root Injury/Percent Consistency Stand Count Percent Lodging Yield European Corn Borer Evaluation Tables Ames Pioneer Optimum AcreMax II for European corn borer Stand Count... 3 ECB Whorl Injury... 3 ECB Tunneling (cm) Yield Corn Earworm Evaluation Tables Ames Dow AgroSciences smartstax vs. HXX & RR2 corn traits study Stand Count Percent Damaged Kernels Kernel Area Consumed (cm 2 ) Total Damaged Ears Monsanto smartstax features and benefits study Stand Count... 3 Percent Damaged Kernels... 3 Kernel Area Consumed (cm 2 )... 3 Total Damaged Ears Appendix I - Agronomic Information Appendix II Weather Data Appendix III - Materials Tested Appendix IV - Research Pictures...

5 20 IOWA EVALUATION OF INSECTICIDES AND PLANT INCORPORATED PROTECTANTS CORN PESTS RESEARCH PROJECT DEPARTMENT OF ENTOMOLOGY AMES, IOWA Project Leader: Aaron Gassmann Agricultural Specialist: Patrick Weber Postdoctoral Research Associate: Jennifer Petzold-Maxwell Graduate Research Assistants: Lab Research Assistant: Mike Dunbar Ryan Keweshan Eric Clifton Amanda Hoffmann Jeremy Bolles Technical Assistants: University Research Farm Superintendents: Charlie Ethington James Terry Lee Koenigsfeld Phil Johnson Walter Wohlford-Wessels Kent Berns Ryan Rusk Kenneth Pecinovsky Kevin VanDee

6 2 INTRODUCTION The corn rootworm species, which includes the western corn rootworm Diabrotica virgifera virfigera and northern corn rootworm D. barberi, are the most damaging pests of corn Zea mays in the Unites States Corn Belt. Eggs are laid in the soil during the fall and hatch the following spring. Larval feeding on corn roots in June may diminish yield by reducing plant growth and drought tolerance and by imposing harvesting losses due to plant lodging. Adult emergence from the soil is underway by early July and continues through late summer. Additional crop losses can be caused by the beetles feeding on the female flowers (silks) and on soft doughy kernels. In Iowa, crop rotation, where it fits cropping practices, remains the preferred method of control. However, it is also economically feasible to protect corn roots with insecticides and plant-incorporated protectants (transgenic seedcorn that contains a gene from the naturally occurring soil bacterium Bacillus thuringiensis (Bt). In additional to corn rootworm, several above-ground lepidopteran pests feed on corn in Iowa. These include the European corn borer Ostrinia nubilalis, black cutworm Agrotis ipsilon, fall armyworm Spodoptera frugiperda, and corn earworm Helicoverpa zea. Over the last decade, Bt corn varieties have been made commercially available to protect corn from these pests. OBJECTIVE The goal of this research program is to serve Iowa agriculture by monitoring and evaluating the performance of registered commercial insecticides and transgenic corn hybrids. To achieve this goal, we maintain a viable, proactive, progressive and scientifically sound product evaluation program. TESTING PROCEDURES AND EVALUATIONS Field Sites: Product efficacy study plots were established at six Iowa locations in 20. Corn rootworm (CRW) research fields are continually maintained on University farms located at: Ames, Johnson Farm; Crawfordsville, S.E. Research and Demonstration Farm; Nashua, N.E. Research and Demonstration Farm; Sutherland, N.W. Research and Demonstration Farm and Lewis, S.W. Research and Demonstration Farm. Each research field is divided into two sections which annually alternate as test plot and late planted trap crop. The seed planted for the trap crop is a mixed maturity blend with a greater proportion of latematuring varieties. This trap crop constitutes a favorable environment for adult female CRW late in the season when other fields are maturing. In addition, we conducted corn rootworm (CRW) research on two second year corn fields at two Ames locations (ISU- Old Dairy Farm and ISU South Dairy Farm). Also, we conducted corn rootworm (CRW) research on a continuous corn field (since 2003) in a private grower s field in northeastern Iowa. Table : Lists the research conducted at each location, target pest, and other general plot information.

7 3 Corn Rootworm Studies Field plot design: The experimental design in most studies was a randomized complete block. Treatments in the yield studies at both Crawfordsville (Tables 7-9) and Nashua (Tables 28-3) were paired rows 7-feet in length with four replications. Treatments for the Pioneer dual pyramid study for corn rootworm at Ames (Tables -7) were four rows wide with 0 feet per row with four replications. The Pioneer CMRA study (Tables 2-) at Ames were eight row treatments that were 2 feet in length with six replications. The treatments in the Monsanto corn rootworm studies at Ames (Tables 8-0), northeast Iowa (Tables 2-27) and Nashua (Tables 32-3), were eight row treatments with 0 feet per row with four replications. The Monsanto genuity VT3P study in Ames (Tables -2) were eight row treatments with 00 feet per row with one replication. The Monsanto smartstax features and benefits study in Ames (Tables 3-) were eight row treatments with 200 feet per row with one replication. Treatments for the Syngenta experimental hybrid efficacy studies at Ames-ISU Old Dairy Farm (Table ) and Ames-ISU South Dairy Farm (Table 6) were four row wide treatments that were 20 feet in length with four replications. The Monsanto smartstax RIB study in Crawfordsville (Tables 20-2) were eight row treatments with 00 feet per row with one replication. The Monsanto smartstax features and benefits study in Crawfordsville (Tables 22-23) were eight row treatments with 00 feet per row with one replication. Application techniques: Seeds were pre-bagged and planted with a four-row John Deere Max Emerge TM 700 integral planter that had 30 inch row spacings. The standard planter fiberglass seed hoppers with attached finger pickup mechanism, were replaced with modified units. On the new units, the metal plate that covered the fingers had been replaced with a 7/8-inch, clear Plexiglas plate. Inserted through the Plexiglas was a small stainless steel cylinder. The cylinder was positioned to deliver seed to the pickup fingers. At the beginning of each replication pre-bagged seeds were dumped into the steel cylinder. At the beginning of each replication, a hydraulic motor (attached to the planter s drive shaft) was activated to deliver seed immediately into the ground. At the end of each replication, this same hydraulic motor was activated to expel any unplanted seed. Plant-incorporated protectants: Plantincorporated protectants were evaluated in corn hybrids producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). These included the YieldGard hybrids of Monsanto that produce the Bt toxin Cry3Bb and the Herculex hybrids of Pioneer and Dow AgroSciences that produce the binary Bt toxin Cry3/Cry3. Seed treatments: All the DeKalb hybrids evaluated in the yield studies (Crawfordsville, Tables 7-9; Nashua, Tables 28-3) were treated by the respective company with Poncho 00. All the Mycogen hybrids evaluated in the yield studies (Crawfordsville, Tables 7-9; Nashua, Tables 28-3) were treated by the respective company with Cruiser

8 Extreme 20. For the Pioneer dual pyramid study for corn rootworm, (Ames, Tables -7) all treatments were treated with Cruiser 20 except one treatment of the pure stand of RR2 where the seeds were treated with Poncho 20/VOTIVO. For the Pioneer CMRA study (Ames, Tables 2-) for corn rootworms and Pioneer Optimum AcreMax II study (Ames, Tables 36-39) for European corn borer all hybrids were treated with Cruiser 20. For the blended refuge seeds (RR2) planted in the Pioneer dual pyramid study for corn rootworm all seeds were commercially treated with Poncho 20/VOTIVO. In the Pioneer Optimum AcreMax II study for European corn borer all blended refuge seeds (RR2) planted were treated with Cruiser 20 insecticidal seed treatment. For the Monsanto studies at Nashua (Tables 32-3), Ames (Tables 8-0) and northeast Iowa (Tables 2-27), Monsanto Genuity VT3P study in Ames (Tables -2), Monsanto smartstax features and benefits study at Ames (Tables 3-) and Crawfordsville (Tables 22-23), and Monsanto smartstax RIB study in Crawfordsville (Tables 20-2) the Poncho 20 seed treatment was commercially applied. The Syngenta experimental hybrid efficacy studies at Ames-Old Dairy Farm (Tables ) and Ames-South Dairy Farm (Tables 7) these hybrids were treated with Cruiser Extreme 20. All these seed treatments target secondary soil-borne pests, such as wireworm and seedcorn maggot. For some treatments, plant-incorporated protectants were combined with conventional insecticides. Granular application treatments: Force 3G granular insecticide was applied to one treatment in the Pioneer dual pyramid study for corn rootworm study and six treatments in each of the Syngenta experimental hybrid efficacy studies at Ames-Old Dairy Farm and Ames-South Dairy Farm with modified Noble metering units mounted on the planter. Aztec 2.G granular insecticide was applied to two treatments in the Yield studies at both Crawfordsville and Nashua with modified Noble metering units mounted on the planter. The Noble units were calibrated in the laboratory to accurately deliver material at a tractor speed of mph. Plastic tubes directed the granular treatments to either a 7- inch band ahead of the closing wheels (T-band, All-Terrain Banders), or to the seed furrow, placing all the insecticide in-furrow (Furrow). Eleven-inch polybristle skirts were attached to the frame and the frame positioned so the bristle tips touched the ground. Each row was constantly monitored to ensure that insecticides were correctly applied at all times. Final incorporation was accomplished with drag chains mounted behind the closing wheels. SmartBox TM application treatments: SmartChoice G and Counter 20G, treatments were applied with modified SmartBox TM metering units. These products were applied as ounces per 000 row feet. The commercial SmartBox TM were removed from their large-base containers and sandwiched between a flat metal plate on the bottom and a custom-made, threaded plastic cap on the top. The bottom plate had been fabricated so that it could slide in and out of the same planter mounting brackets used for the Noble units. An inverted 000 ml Nalgene bottle, screwed into the top cap provided a secure and sealed container for

9 insecticide. A short plastic tube attached to the dispenser opening of the metering unit could be connected to either the planter s T-band or Furrow tubes. The two controllers mounted in the tractor cab were used to operate the SmartBox TM metering units. All treatments were applied at mph using the fixed speed mode on the SmartBox TM controllers. European Corn Borer Study Field plot design: The experimental design in the Pioneer Optinum AcreMax II study for European corn borer was a randomized complete block with four replications respectively. Five treatments were evaluated (Pioneer HX alone, Pioneer RR2 hybrid alone, Pioneer HX x YGCB (pyramid) alone, and with a % and 0% blended refuge seeds (RR2). These treatments were four rows wide, 0-feet in length. Plots were cut down to 38 feet of row for alley purposes. In the blended refuges, a gene check was used to identify refuge plants (rows and : blended refuge treatments only). A total of twenty four three-plant clusters were marked with stakes for rows and of the % blended refuge treatment and the 0% blended refuge treatment. Two six-plant clusters were marked with stakes within rows and for the treatments of HX hybrid alone, HX x YGCB (pyramid) and RR2 hybrid alone for all four replications. Within the blended refuge treatments the plant clusters consisted of either: Refuge-Bt-Bt or Bt-Bt-Refuge. Application techniques: The ECB neonates were obtained from the USDA-ARS Corn Insects and Crop Genetics Research Laboratory. We obtained four bottles of neonates that were mixed with corn grit. We infested with first-generation ECB on 7 June and 2 June. The inoculum was applied into the whirl. When infesting with second-generation ECB on 9 August and 6 August, the inoculum was applied into the ear-leaf collar. Inoculum was equal to 00 ECB per plant and administered with bazooka inoculators. On July, the ECB injury was scored. A total of 288 plants were evaluated. We used the Pioneer 9- rating scale for evaluating firstgeneration ECB whorl injury. On 6 October and 7 October, tunneling was evaluated by splitting stalks into three parts (top, shank, and bottom) with a curved linoleum knife. All three plant clusters in the two blended refuge treatments and all six plant clusters in the three pure stand treatments were evaluated for a total of 288 plants. Total tunnel length (cm) was scored for each plant part and averaged for each treatment. Corn Earworm Study Field plot design: The experimental design in this Dow AgroSciences smartstax vs Herculex XTRA and RR2 corn study in Ames was a randomized complete block with four replications. The treatments consisted of Mycogen RR2 corn, Mycogen smartstax and Mycogen Herculex XTRA. These treatments were four rows wide, 20-feet in length. Application techniques: The st CEW instar larvae were obtained from Benzon Research from Carlisle, Pennsylvania. Prior to infestation, corn ear shoot bags were placed over the top ear shoot of all

10 6 plants in row 2 and 3 of each plot. On August a #2 camel hair brush was used to infest 0 ears per row (of rows 2 and 3) to the silks with, st instar larvae. One summer Technical Assistant was assigned one replication of treatments to infest. On 8 August ( days after infestation) 0 randomly selected ears were sampled from the two center rows of each plot. From these ears we counted the numbers of kernels damaged, in addition, we measured with a caliper (in millimeters) the average kernel cap size, both length and width. In addition, we counted the number of rows of kernels around the ear and the number of kernels in an average row length. From these numbers collected, we calculated the percentage of damaged kernels per ear and per treatment. Corn Earworm Study Field plot design: The experimental design in this Monsanto smartstax features & benefits study was a randomized complete block with four replications. The treatments consisted of Pioneer HXX (Pioneer 3F3PDR), Syngenta Agrisure (N3C-3), DeKalb smartstax (DKC -09), DeKalb RR2 (DKC -08) with Aztec 2.G 0.2 ounces ai/000 rowft, Syngenta GT Agrisure (N3-W2) with Aztec 2.G 0.2 ounces ai/000 rowft, Pioneer RR2 (3F37) with Aztec 2.G 0.2 ounces ai/000 rowft and DeKalb RR2 (DKC - 08) These treatments were eight rows wide, 200-feet in length. Application techniques: The st CEW instar larvae were obtained from Benzon Research from Carlisle, Pennsylvania. Prior to infestation, corn ear shoot bags were placed over the top ear shoot of 0 plants in row 2 and 7 of each plot. On 0 August a #2 camel hair brush was used to infest 0 ears per row (of rows 2 and 7) to the silks with, 2nd instar larvae. One summer Technical Assistant was assigned one replication of treatments to infest. On 2 August ( days after infestation) 0 randomly selected ears were sampled total from rows 2 and 7 of each plot. From these ears we counted the numbers of kernels damaged, in addition, we measured with a caliper (in millimeters) the average kernel cap size, both length and width. In addition, we counted the number of rows of kernels around the ear and the number of kernels in an average row length. From these numbers collected, we calculated the percentage of damaged kernels per ear and per treatment. Corn Rootworm Larval Evaluations Stand counts: The number of plants in either 7., or 20 row-ft was recorded (note distance in foot notes of stand count tables). These were taken early and late in the growing season. The number of plants in 38 row-ft was recorded for both the Pioneer Dual Pyramid study for corn rootworm and Pioneer Optimum AcreMax II study for European corn borer. These were taken early and at Physiological maturity during the growing season. Root-Injury: Since CRW beetle emergence was delayed by 0- days, we delayed starting root digging until the first of the month of August. After the majority of corn rootworms had finished feeding, roots were dug from 8/0/20

11 7 through 8/7/20. Five root systems total were dug per replication from each insecticide treatment (except from the My-9%smartstax/%non-Bt treatment (refuge in the bag)) where nine root systems (6 smartstax + 3 non-bt) total were dug per replication from the tworow yield studies (Crawfordsville, Table 7 & Nashua, Table 28). For the Monsanto corn rootworm studies at Nashua (Table 32), Ames (Table 8) and northeast Iowa (Table 2), five root systems total were dug from each insecticide treatment out of rows 2 and 7. With the Pioneer CMRA study, six roots total were dug per treatment from rows two and seven (Ames, Table 3). With the Pioneer dual pyramid study for corn rootworm, we dug 8 root systems per treatment from the four pure stand treatments (HXX + MIR60, RR2 Hybrid + Force 3G at oz/000feet of row, RR2 hybrid with Poncho 20/VOTIVO insecticidal seed treatment, RR2 hybrid with Cruiser 20 insecticidal seed treatment. 72 root systems per treatments were dug from the two blended refuge treatments (HXX + MIR60 (pyramid), with % blended refuge seeds-p03r, HXX + MIR60 (pyramid) with 0% blended refuge seeds-p03r). Roots were dug out of rows and only. With the Syngenta experimental hybrid efficacy study, a total of six root systems total were dug from rows one and four of each treatment (Ames-Old Dairy Farm- (Table ) and Ames-South Dairy Farm-(Table 6). Prior to leaving the field, the roots were marked with a permanent marker with the plot number, study name and location. Excess soil was removed in the field as well. In Ames, roots were soaked in water and then a pressurized water spray was used to remove the remaining soil. Roots were then laid out by replications and were then evaluated for rootworm feeding injury on the following Iowa State Node-Injury Scale (0-3): Node-Injury Scale (0-3): No feeding damage (lowest rating that can be given) One node (circle of roots), or the equivalent of an entire node, eaten back to with-in ~ ½ inches of the stalk (soil line on the 7 th node) Two nodes eaten Three or more nodes eaten. (Highest rating that can be given) Damage in-between complete nodes eaten is noted as the percentage of the node missing, i.e..0 = ½ nodes eaten, 0.2 = ¼ of one node eaten, and so on. The linear node-injury scale allows injury to be expressed intuitively and has proved useful in evaluating minor injury, especially with transgenic seed corn. For an interactive guide to the Node-injury scale, see the Iowa State University Entomology web site at: rootworm/nodeinjury/nodeinjury.html

12 8 means with an experiment wise error rate of P < 0.0. COMMENTS ON INSECTICIDES AND PLANT INCORPORATED PROTECTANTS (PIP) PERFORMANCE Product consistency: The percent product consistency was also calculated for each treatment. Product consistency equals the percentage of times a treatment limited feeding injury to 0.2 node or less (greater injury can result in economic yield loss, especially when plants are moisture stressed). Lodging counts: A plant was considered lodged if it was leaning at least 30 degrees from vertical. These were taken at harvest time along with final stand count numbers (note distance in footnotes of lodging count tables). Yields: Studies that were taken to yield were machine harvested with a modified John Deere 90 plot combine or John Deere 90. Weights (pounds) and percent moisture were recorded from either a HarvestMaster brand or Shivvers brand 00 moisture meter plot harvest data collection system. These measurements were converted to bushels per acre of No. 2 shelled corn (6 lbs. per bushel) at % moisture in an Excel worksheet. All data were analyzed with standard ANOVA procedures using SAS Enterprise Guide.2. When a significant treatment effect was present pairwise comparisons made among Tables list treatment rates as ounces active ingredient per 000 row foot unless otherwise indicated in the footnotes. CORN ROOTWORM EVALUATIONS AMES (Johnson farm) Pioneer CMRA study (Tables 2-): Both the Herculex XTRA treatments (with and without Bio-insecticide) had significantly less node-injury than the two Herculex treatments (Table 2). The Herculex alone treatment had a significantly lower stand count than the other three treatments (Table 3). No significant differences in yield were detected (Table ). Pioneer dual pyramid yield and efficacy for corn rootworm (Tables - 7): No significant differences were noted for stand counts (Table ). Root injury was lowest and product consistency greatest for RR2 with Force 3G. Product consistency was lowest for RR2 in the 0% blended refuge treatment. In general the pyramid had lower root injury and higher product consistency than RR2, except for RR2 with Force 3G (Table 6). The pure stand of RR2 with Poncho 20/VOTIVO insecticidal seed treatment was the highest yielding and was significantly greater than the pure stand of RR2 with the Cruiser20 insecticidal seed treatment and the pyramid with a 0% blended refuge. (Table 7).

13 9 AMES (Johnson farm) Monsanto corn rootworm study (Tables 8-0): The Mycogen Herculex XTRA treatment was the same as the DeKalb RR2 hybrid treatment but was significantly different than all other treatments for node-injury (Table 8). Regarding product consistency, the Mycogen Herculex XTRA treatment was the same as the smartstax treatment but both were significantly different than the remainder of the treatments (Table 8). With stand counts all treatments were the same except the DeKalb YieldGard VT3 + Force overtop and DeKalb RR2 hybrid which were significantly different from each other (Table 9). No significant differences in yield were detected (Table 0). Monsanto genuity VT3P study (Tables -2): No significant differences were noted for stand counts (Table ). No significant differences in yield were detected (Table 2). Monsanto smartstax features and benefits study (Tables 3-): No significant differences were noted for stand counts (Table 3). No significant differences in yield were detected (Table ). AMES (Old Dairy Farm) Syngenta experimental hybrid efficacy study (Tables ): Rootworm pressure was low but we did see a significant difference with the Non-Bt hybrid showing greater node-injury compared to the other treatments (Table ). AMES (South Dairy Farm) Syngenta experimental hybrid efficacy study (Tables 6): Rootworm pressure was low but we did see a significant difference with the Non-Bt hybrid showing greater node-injury compared to the other treatments (Table 6). CRAWFORDSVILLE (S.E. R&D FARM) Yield study (Tables 7-9): Node injury was significantly higher for the two isoline treatments (checks) than all other treatments. The treatment of Mycogen Herculex HXX with SmartChoice-SB insecticide In-furrow over top was the same as the DeKalb Smartstax with Aztec 2.G overtop treatment and the DeKalb Smartstax with Aztec 2.G overtop was the same as Mycogen HXX plus Force 3G overtop and the Mycogen Smartstax treatments (Table 7). Regarding stand counts, the Mycogen Isoline hybrid was the same as all treatments except the Mycogen 9% Smartstax/%Non-Bt which was significantly different (Table 8). With yield the Mycogen Isoline hybrid was the same as all other treatments except the Mycogen HXX with Force 3G and SmartChoice G SmartBox applied Infurrow overtop (Table 9). Monsanto smartstax RIB study (Tables 20-2): No significant differences were noted for stand counts (Table 20). No significant differences in yield were detected either (Table 2). Monsanto smartstax features and benefits study (Tables 22-23): The Agrisure hybrid (Agrisure 3) treatment was significantly different than all other treatments for stand counts

14 0 (Table 22). No significant differences in yield were detected (Table 23). Northeast Iowa Monsanto corn rootworm study (Tables 2-27): There was heavy CRW pressure at this site. The DeKalb Isoline Hybrid (DKC -92) treatment had the highest node-injury followed by the DeKalb YieldGard VT3 hybrid (DKC 6-60) followed by the Mycogen Herculex Hybrid (DKC 2K92) treatment and finally the DeKalb Isoline Hybrid (DKC -92) treatment with Force overtop. These four treatments were significantly different than all the other treatments (Table 2). No significant differences were noted for stand counts (Table 2). The DeKalb Isoline Hybrid (DKC -92) and the DeKalb YieldGard VT3 hybrid (DKC 6-60) treatments had significantly greater percent lodging than all other treatments (Table 26). No significant differences in yield were detected (Table 27). NASHUA (N.E. R&D FARM) Yield study (Tables 28-3): The treatment of DeKalb-Smartstax with Aztec 2.G overtop In-furrow had significantly less node-injury than all ten other treatments (Table 28). No significant differences among stand counts were noted (Table 29). Regarding percent lodging, the DeKalb Isoline and Mycogen Isoline hybrid treatments were significantly different than all other treatments (Table 30). For yield, the DeKalb Isoline and Mycogen Isoline hybrid treatments were significantly different than all other treatments (Table 3). Monsanto corn rootworm study (Tables 32-3): The smartstax hybrid (DKC 6-6) treatment had significantly less node-injury than all other treatments. Both the YieldGard VT3 hybrid (DKC 6-60) treatment and DeKalb Isoline (DKC 6-72) treatment with either Aztec or Force overtop had significant less node-injury than either one of these hybrids alone (Table 32). The smartstax hybrid (DKC 6-6) treatment had significantly lower stand count compared to the Mycogen Herculex hybrid (DKC 2K92) and DeKalb Isoline hybrid (DKC 6-72) plus Force applied overtop treatments (Table 33). The DeKalb Isoline Hybrid (DKC 6-72) treatment had significantly greater percent lodging than all other treatments (Table 3). For yields, the Mycogen Herculex Hybrid (DKC 2K92) treatment was significantly greater than all other treatments in this study (Table 3). EUROPEAN CORN BORER EVALUATIONS AMES (Johnson farm) Pioneer Optimum AcreMAX II study for European corn borer (Table 36-39): Stand counts were similar among all treatments (Table 36). With whorl injury, the RR2 plants within the 0% blended refuge were significantly greater than the other treatments (Table 37). Tunneling of ECB was significantly lower on HX and the pyramid (HX x YGCB) than on RR2 either alone or in a blended refuge (Table 38). No significant differences in yield were detected (Table 39). CORN EARWORM EVALUATIONS AMES (Johnson farm) Dow Agro Sciences Smartstax vs. Herculex XTRA and RR2 corn traits

15 study (Tables 0-2): The HXX/RR2 treatment had significantly lower stand count than the RR2 but was similar to the smartstax treatment (Table 0). Regarding damaged kernels per ear, percent damaged per ear, kernel area consumed (cm) and total damaged ears both the RR2 and HXX/RR2 treatments were significantly higher than the smartstax treatment (Tables -2). AMES (Johnson farm) Monsanto smartstax features and benefits study (Tables 3-): Regarding damaged kernels per ear, percent damaged per ear, kernel area consumed (cm) and total damaged ears, both the Agrisure hybrid (Agrisure 3) and smartstax (DKC -09) treatments were significantly lower than all other treatments (Tables 3-). CALIBRATION INFORMATION All Noble units were laboratory calibrated and units were randomly spot-checked in the field prior to planting. SmartBox TM units were calibrated on the planter in accordance with the SmartBox Operator s Manual instructions. During calibration and planting, the flowability of each formulation was noted, as well as any other calibration problems. There were no calibration or delivery problems with any treatment. AGRONOMIC INFORMATION, WEATHER DATA AND MATERIALS TESTED AND PICTURES OF RESEARCH PROJECTS Weather data from the test site or the nearest Iowa Climatological Station are listed in Appendix II. Information on materials tested is listed in Appendix III. Pictures of various activities of research projects are listed in Appendix IV. RESEARCH SUPPORT Many thanks to the Iowa Agriculture and Home Economics Experiment Station and the following companies for providing support for the evaluation of insecticides and plant-incorporated protectants (Corn Pests Research Project): AMVAC Chemical Corporation, Dow AgroSciences, Monsanto, Pioneer Hi-Bred International, Inc., Syngenta. WAIVER OF ENDORSEMENT This report deals with the relative ability of each treatment to protect corn from damage by soil insects. This information is not presented to endorse the use of any product and the name of Iowa State University should not appear in any advertising without prior written consent. Iowa State University, their respective officers, agents, or employees, have not made, and do not hereby make, any representation, warranty or covenant with respect to the use of these test results, nor will they be liable for any damages, losses, or claims, including those of an incidental or consequential nature, arising out of the use of these test results. Agronomic information and field insecticide history for each test plot location are listed in Appendix I.

16 2 Table. Iowa evaluation of insecticides and plant-incorporated protectants. Experimental Unit Size Target Pest and Table Entries/ Row # Test Location Type of Studies Numbers Test Length (ft) Reps Corn Rootworms Ames )Pioneer CMRA study 2)Pioneer dual pyramid efficacy and yield study 3)Monsanto corn rootworm study )Monsanto genuity VT3P seed blend study )Monsanto smartstax features & benefits study 6)Syngenta experimental hybrid efficacy study 7)Syngenta experimental hybrid efficacy study Crawfordsville )Yield study )Monsanto smartstax RIB )Monsanto smartstax features & benefits study Northeast Iowa )Monsanto corn rootworm study Nashua )Yield study 2)Monsanto corn rootworm study European Corn borer Ames Corn Earworm Ames )Pioneer Optimum AcreMax II study (SB) )Dow AgroSciences smartstax vs HXX & RR2 traits study (SB) 2)Monsanto smartstax features & benefits study All Studies were conducted on trap crop corn ground unless noted otherwise in parenthesis. (SB) = test conducted on ground that was planted to soybeans in 200.

17 3 Table 2. Average root-injury and percent product consistency for the Pioneer CMRA study: Ames, IA. Node- Product Treatment 2 Injury 3,, Consistency 6,7 HXX with Bio-insecticide 8,9,0 0.06a 9a HXX 0.07a 97a HX with Bio-insecticide 8,9,0 0.6 b 80ab HX 0.27 c 6 b Planted May, 20; node injury evaluated August, 20 2 HXX = Herculex XTRA hybrid (Pioneer P39XR); HX = Herculex hybrid (Pioneer P39HR) 3 Chemical and check means based on 36 observations (6 roots/2 rows x 6 replications) Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 6 Product consistency = percentage of times nodal injury was 0.2 (¼ node eaten) or less 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis bacteriophora bioinsecticide (nematodes) solution applied at a rate of 00 nematodes per square inch. 9 Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter 0 Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn plants and 2 inches deep Table 3. Average stand count for the Pioneer CMRA study: Ames, IA. Stand Treatment 2 Count 3, HX with Bio-insecticide,6,7 3.7a HXX with Bio-insecticide,6,7 3.00ab HXX 3.7ab HX 3.00 b Planted May, 20; evaluated June 7 and September 2, 20 2 HXX = Herculex XTRA hybrid (Pioneer P39XR); HX = Herculex hybrid (Pioneer P39HR) 3 Means based on 2 observations ( row treatment x 20 row-feet/treatment x 6 replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis bacteriophora bioinsecticide (nematodes) solution applied at a rate of 00 nematodes per square inch. 6 Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter 7 Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn plants and 2 inches deep

18 Table. Average yield for the Pioneer CMRA study: Ames, IA. Treatment 2 Yield 3,, HX with Bio-insecticide 6,7,8 202 HXX with Bio-insecticide 6,7,8 9 HXX 88 HX 83 Planted May, 20; Harvested (mechanically) October 3, 20 2 HXX = Herculex XTRA hybrid (Pioneer P39XR); HX = Herculex hybrid (Pioneer P39HR) 3 Means based on 6 observations (-rows x 20 row-feet/treatment x 6 replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to% Moisture 6 Metarhizium anisopliae bioinsecticide (fungus) applied at 208 grams per plot; Steinernema carpocapsae and Heterorhabditis bacteriophora bioinsecticide (nematodes) solution applied at a rate of 00 nematodes per square inch. 7 Metarhizium anisopliae broadcast over plots by hand before planting then were incorporated by tiller shortly thereafter 8 Steinernema carpocapsae and Heterorhabditis bacteriophora were applied along each row approximately 6-9 inches from corn plants and 2 inches deep Table. Average stand count for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA. Stand Treatment 2,3 Count,,6 HXX + MIR60 (Pure stand) 68.7 HXX + MIR60 (9% HXX + MIR60; % RR2 blended refuge) 67.7 HXX + MIR60 (90% HXX + MIR60; 0% RR2 blended refuge) 67.7 RR2 + Force 3G - 0.2/T-band 67.2 RR2 (Pure stand) with Poncho 20/VOTIVO 67.2 RR2 (Pure stand) with Cruiser Planted May 0, 20; evaluated June 6, 20 2 HXX + MIR60 = Dual Pyramided hybrid (Pioneer product TQ3882); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P03R) 3 HXX = Herculex XTRA technology. Herculex Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred.. Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company Force 3G is a registered trademark of a Syngenta Group Company Means based on 6 observations ( row treatment x 38 row-feet/treatment x replications) Insecticide listed as ounces a.i. per,000 row-feet; T-band = Insecticide applied at planting time 6 No significant differences between means (ANOVA, P < 0.0)

19 Table 6. Average root-injury and percent product consistency for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA, Node- Product Treatment 2.3, Injury,6 Consistency 7,8 RR2 (Pure stand) + Force 3G - 0.2/T-band 0 0.0a 98a HXX + MIR60 (90% HXX + MIR60) 0.0a 96a HXX + MIR60 (Pure stand) 9 0.ab 88ab HXX + MIR60 (9% HXX + MIR60) b 73ab RR2 (Pure stand) with Poncho20/VOTIVO bc 7ab RR2 blended refuge (0%) 0.30 cd 0ab RR2 blended refuge (%) 0.33 cd 7 b RR2 (Pure stand) with Cruiser d b Planted May 0, 20; node injury evaluated August, 20 2 HXX + MIR60 = Dual Pyramided hybrid (Pioneer product TQ3882); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P03R) 3 HXX = Herculex XTRA technology. Herculex Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company Force 3G is a registered trademark of a Syngenta Group Company Insecticide listed as ounces a.i. per,000 row-feet; T-band = Insecticide applied at planting time Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 6 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 7 Product consistency = percentage of times nodal injury was 0.2 (¼ node eaten) or less 8 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 9 For the pure stand treatment of HXX + MIR60, 8 Bt roots were dug (2 roots/2 rows x replications) 0 For the pure stand treatments of RR2, 8 non-bt roots were dug (2 roots/2 rows x replications) For the RR2 blended refuge treatments (% & 0%), 2 non-bt roots were dug (6 roots/2 rows x replications) 2 For the pyramided treatments (90% & 9%), 8 Bt roots were dug (2 roots/2 rows x replications) Table 7. Average yield for the Pioneer dual pyramid efficacy and yield study for corn rootworm: Ames, IA. Treatment 2,3, Yield,6,7 RR2 (Pure stand) with Poncho 20/VOTIVO 20a RR2 + Force 3G - 0.2/T-band 8ab HXX + MIR60 (Pure stand) 8ab HXX + MIR60 (9% HXX + MIR60; % RR2 blended refuge) 8ab RR2 (Pure stand) with Cruiser bc HXX + MIR60 (90% HXX + MIR60; 0% RR2 blended refuge) 66 c Planted May 0, 20; machine harvested October 3, 20 2 HXX + MIR60 = Dual Pyramided hybrid (Pioneer product TQ3882); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P03R) 3 HXX = Herculex XTRA technology. Herculex Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Herculex is a registered trademark of Dow AgroSciences LLC. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company Force 3G is a registered trademark of a Syngenta Group Company. Insecticide listed as ounces a.i. per,000 row-feet; T-band = Insecticide applied at planting time Means based on observations (2-rows x 38 row-feet/treatment x reps) 6 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 7 Yields converted to% Moisture

20 6 Table 8. Average root-injury and product consistency for the Monsanto corn rootworm study: Ames, IA. Node- Product Treatment 2 Form. Rate 3 Placement Injury,6,7 Consistency 8,9 YGVT3 + Force 3.0G 0.2 T-band 0.0a 9a YGVT3 + Aztec 2.G 0. T-band 0.06a 9a DeKalb-Iso + Aztec 2.G 0. T-band 0.0a 8a DeKalb-Iso + Force 3.0G 0.2 T-band 0.0a 8a YGVT ab 9a Smartstax ab 80 b DeKalb-Iso bc 90a My-HXT c b Planted May 0, 20; evaluated August, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Chemical and check means based on 20 observations ( roots/2 rows x replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0.2 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 9. Average stand count for the Monsanto corn rootworm study: Ames, IA. Stand Treatment 2 Form. Rate 3 Placement Counts,6 DeKalb-Iso a My-HXT ab DeKalb-Iso + Aztec 2.G 0. T-band 3.0abc Smartstax abc DeKalb-Iso + Force 3.0G 0.2 T-band 3.2abc YGVT abc YGVT3 + Aztec 2.G 0. T-band 3.00 bc YGVT3 + Force 3.0G 0.2 T-band 33.0 c Planted May 0, 20; evaluated June 7 and September 2, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time Means based on 32 observations ( rows/ treatment x 7. row-ft/treatment x replications x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.0)

21 7 Table 0. Average yield for the Monsanto corn rootworm study: Ames, IA. Bushels/ Treatment 2 Form. Rate 3 Placement Acre,6,7 My-HXT DeKalb-Iso DeKalb-Iso + Force 3.0G 0.2 T-band 68 YGVT3 + Aztec 2.G 0. T-band 67 YGVT3 + Force 3.0G 0.2 T-band 6 YGVT DeKalb-Iso + Aztec 2.G 0. T-band 9 Smartstax Planted May 0, 20; machine harvested October 3, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on observations (-row treatment x 3 row-feet/treatment x replications) 6 No significant differences between means (ANOVA, P < 0.0) 7 Yields converted to % Moisture Table. Average stand counts for the Monsanto genuity VT3P seed blend efficacy study: Ames, IA. Stand Treatment 2 Counts 3, Genuity VT3P RIB (00% Bt corn) F 36.2 Smartstax (9% Bt corn/% non-bt corn) Smartstax (00% Bt corn) 3.7 Dekalb-RR2 3.2 Genuity VT3P RIB (90% Bt corn/0% non-bt corn) 3.00 Genuity VT3P RIB (80 Bt corn/20% non-bt corn) 3.7 Planted May 0, 20; evaluated June 7 and September 22, 20 2 DeKalb-RR2 = DeKalb brand RR (DKC 6-36); Smartstax (00% Bt corn) = DeKalb brand Smartstax (DKC 6-2); Smartstax (9% Bt corn/% non-bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC6-2JRM); Genuity VT3P RIB (90% Bt corn/0% non-bt corn) = DeKalb VT3P Refuge in a bag (DKC6-97DKL); Genuity VT3P RIB (00% Bt corn) = DeKalb brand VT3P Refuge in a bag (DKC6-97WTM); Genuity VT3P RIB (80 Bt corn/20% non-bt corn) = DeKalb brand VT3P Refuge in a bag (DKC6-97BPY) 3 Means based on 8 observations ( rows/ treatment x 7. row-feet/treatment x 2 evaluations) No significant differences between means (ANOVA, P < 0.0)

22 8 Table 2. Average yield for the Monsanto genuity VT3P seed blend efficacy study: Ames, IA. Bushels/ Treatment 2 Acre 3,, Genuity VT3P RIB (80 Bt corn/20% non-bt corn) 87 Genuity VT3P RIB (90% Bt corn/0% non-bt corn) 73 Genuity VT3P RIB (00% Bt corn) 73 DeKalb-RR2 70 Smartstax (00% Bt corn) 9 Smartstax (9% Bt corn/% non-bt corn) 8 Planted May 0, 20; machine harvested October 2, 20 2 DeKalb-RR2 = DeKalb brand RR (DKC 6-36); Smartstax (00% Bt corn) = DeKalb brand Smartstax (DKC 6-2); Smartstax (9% Bt corn/% non-bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC6-2JRM); Genuity VT3P RIB (90% Bt corn/0% non-bt corn) = DeKalb VT3P Refuge in a bag (DKC6-97DKL); Genuity VT3P RIB (00% Bt corn) = DeKalb brand VT3P Refuge in a bag (DKC6-97WTM); Genuity VT3P RIB (80 Bt corn/20% non-bt corn) = DeKalb brand VT3P Refuge in a bag (DKC6-97BPY) 3 Means based on observation (-row treatment x 9 row-feet/treatment) No significant differences between means (ANOVA, P < 0.0) Yields converted to % Moisture Table 3. Average stand count for the Monsanto genuity smartstax features and benefits study: Ames, IA. Stand Treatment 2 Form. Rate 3 Placement Counts,6 DeKalb-RR2 + Force 3.0G 0.2 T-band 3.2 Smartstax Agrisure + Force 3.0G 0.2 T-band 33.0 DeKalb-RR Pioneer-HXX Agrisure Pioneer-RR2 + Force 3.0G 0.2 T-band 32.0 Planted May 0, 20; evaluated June 7 and September 28, 20 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 3F3PDR); Agrisure = Syngenta Agrisure GT hybrid (N3-W2); DeKalb-RR2 = DeKalb brand RR (DKC -08); Agrisure 2 = Syngenta Agrisure 3 hybrid (N3C-3); Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 3F37) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time Means based on 8 observations ( rows/ treatment x 7. row-feet/treatment x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.0)

23 9 Table. Average yield for the Monsanto genuity smartstax features and benefits study: Ames, IA. Bushels/ Treatment 2 Form. Rate 3 Placement Acre,6,7 Pioneer-RR2 + Force 3.0G 0.2 T-band 62 DeKalb-RR2 + Force 3.0G 0.2 T-band 60 Agrisure + Force 3.0G 0.2 T-band 60 Agrisure DeKalb-RR Smartstax Pioneer-HXX Planted May 0, 20; machine harvested October 2, 20 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 3F3PDR); Agrisure = Syngenta Agrisure GT hybrid (N3-W2); DeKalb-RR2 = DeKalb brand RR (DKC -08); Agrisure 2 = Syngenta Agrisure 3 hybrid (N3C-3); Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 3F37) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on observations (-row treatment x 9 row-feet/treatment) 6 No significant differences between means (ANOVA, P < 0.0) 7 Yields converted to % Moisture Table. Average root-injury and percent product consistency for the Syngenta experimental hybrid efficacy study: Old Dairy Farm, Ames, IA. Node Product Treatment 2,3 Rate Placement Injury 6,7,8 Consistency 9,0 MIR60 + HXRW + HX + Bt a 00a MIR60 + HXRW + HX + Bt + Force 0.2 T-band 0.0a 00a 9% MIR60 + HX + Bt + HXRW / % refuge + Force 0.2 T-band 0.0a 00a MIR60 + Bt + Force 0.2 T-band 0.02a 00a 90% MIR60 + HX + Bt + HXRW / 0% refuge + Force 0.2 T-band 0.02a 00a MIR62 + MIR60 + Bt + Force 0.2 T-band 0.02a 00a 9% MIR60 + HX + Bt + HXRW / % refuge a 00a 90% MIR60 + HX + Bt + HXRW / 0% refuge a 00a MIR60 + Bt a 00a MIR62 + MIR60 + Bt a 92ab Non--Bt + Force 0.2 T-band 0.08a 92ab Non-Bt b 79 b Planted May 8, 20; evaluated August 8, 20 2 MIR60 is the Agrisure RW trait; MIR62 is the Agrisure Viptera trait for above-ground lepidopteran protection 3 Refuge corn does not contain rootworm active Bt but does have Bt; HXRW = Herculex rootworm; Bt = Agrisure CB/LL trait for above-ground lepidopteran protection (e.g., corn borer); HX = Herculex I (lepidopteran protection) Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time 6 Chemical and check means based on 2 observations (6 roots/2 rows x replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 8 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 9 Product consistency = percentage of times nodal injury was 0.2 (¼ node eaten) or less 0 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Force is a Restricted Use Pesticide.

24 20 Table 6. Average root-injury and percent product consistency for the Syngenta experimental hybrid efficacy study: South Dairy Farm, Ames, IA. Node Product Treatment 2,3 Rate Placement Injury 6,7,8 Consistency 9,0 MIR60 + HXRW + HX + Bt a 00 9% MIR60 + HX + Bt + HXRW / % refuge a 00 MIR60 + HXRW + HX + Bt + Force 0.2 T-band 0.03a 00 9% MIR60 + HX + Bt + HXRW / % refuge + Force 0.2 T-band 0.03a 00 90% MIR60 + HX + Bt + HXRW / 0% refuge + Force 0.2 T-band 0.03a 00 MIR62 + MIR60 + Bt + Force 0.2 T-band 0.0a 92 90% MIR60 + HX + Bt + HXRW / 0% refuge a 00 MIR60 + Bt + Force 0.2 T-band 0.06a 96 MIR62 + MIR60 + Bt a 00 MIR60 + Bt a 96 Non--Bt + Force 0.2 T-band 0.08a 92 Non-Bt b 83 Planted May 9, 20; evaluated August 9, 20 2 MIR60 is the Agrisure RW trait; MIR62 is the Agrisure Viptera trait for above-ground lepidopteran protection 3 Refuge corn does not contain rootworm active Bt but does have Bt; HXRW = Herculex rootworm; Bt = Agrisure CB/LL trait for above-ground lepidopteran protection (e.g., corn borer); HX = Herculex I (lepidopteran protection) Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time 6 Chemical and check means based on 2 observations (6 roots/2 rows x replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 8 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 9 Product consistency = percentage of times nodal injury was 0.2 (¼ node eaten) or less 0 No significant differences between means (ANOVA, P < 0.0) Force is a Restricted Use Pesticide. Node- Product Treatment 2,3 Form. Rate Placement Injury 6,7,8 Consistency 9,0 My-HXX + SmartChoice-SB G 0.8 In-Furrow 0.09a 90a DeKalb-Smartstax + Aztec 2.G 0. In-Furrow 0.8ab 7ab My-HXX + Force 3.0G 0.2 In-Furrow 0.2 bc 60ab My-Smartstax bc 6ab My-HXX + Counter-SB 20G 0.90 In-Furrow 0. cd abc My-HXX d 2abc My-Iso + Force 3.0G 0.2 In-Furrow 0.6 de 2 bc DeKalb-Iso + Aztec 2.G 0. In-Furrow 0.77 de bc My-9%SSTX/%Non-Bt e 8 bc DeKalb-Iso f 0 c My-Iso g 0 c Planted May, 20; evaluated August, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-feet In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Chemical and check means (except My-9%SSTX/%Non-Bt treatment) based on 20 observations ( roots/2 rows x replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 8 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 9 Product consistency = Percentage of times nodal injury was 0.2 (¼ node eaten) or less 0 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) For the refuge in the bag treatment (My-9%SSTX/% Non-Bt), mean based on 36 observations (9 roots/2 rows (6 Smartstax (3 adjacent roots and 3 distant roots to a Non-Bt plant) + 3 Non-Bt) x replications)

25 2 Table 8. Yield study: Crawfordsville, IA. Stand Treatment 2,3 Form. Rate Placement Count 6,7 My-9%SSTX/%Non-Bt a My-HXX + SmartChoice-SB G 0.8 In-Furrow 3.2ab My-Smartstax ab DeKalb-Iso ab DeKalb-Smartstax + Aztec 2.G 0. In-Furrow 3.0ab My-HXX + Force 3.0G 0.2 In-Furrow 3.0ab My-Iso + Force 3.0G 0.2 In-Furrow 3.2ab My-HXX ab DeKalb-Iso + Aztec 2.G 0. In-Furrow 33.7ab My-HXX + Counter-SB 20G 0.90 In-Furrow 33.0 b My-Iso b Planted May, 20; evaluated June 8 and September 30, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-feet In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 6 observations (2-row treatment x 7. row-feet/treatment x replications x 2 evaluation dates) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 9. Yield study: Crawfordsville, IA. Bushels/ Treatment 2,3 Form. Rate Placement Acre 6,7,8 My-HXX + SmartChoice-SB G 0.8 In-Furrow a My-HXX + Force 3.0G 0.2 In-Furrow 2a My-HXX + Counter-SB 20G 0.90 In-Furrow 2ab My-9%SSTX/%Non-Bt ab My-Smartstax ab DeKalb-Iso + Aztec 2.G 0. In-Furrow 39ab My-HXX ab DeKalb-Iso ab DeKalb-Smartstax + Aztec 2.G 0. In-Furrow 9ab My-Iso + Force 3.0G 0.2 In-Furrow 8ab My-Iso b Planted May, 20; machine harvested October 7, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-feet In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on observations (2-row treatment x 69 row-feet/treatment x replications) 7 No significant differences between means (ANOVA, P < 0.0) 8 Yields converted to% Moisture

26 22 Table 20. Average stand count for the Monsanto smartstax refuge in a bag study: Crawfordsville, IA. Stand Treatment 2 Form. Rate 3 Placement Counts,6 DeKalb-RR2 + Aztec 2.G 0. T-band 3.2 Smartstax RIB (9%Bt/%non-Bt) DeKalb-RR Smartstax Pioneer-HXX Pioneer OAM Planted May, 20; evaluated June 8 and September 30, 20 2 Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer OAM = Pioneer Optimum AcreMax (Pioneer P028XR); Smartstax (9% Bt corn/% non-bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC-08JRM); Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer P028AMI); DeKalb-RR2 = DeKalb brand RR (DKC -08) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on 8 observations ( rows/ treatment x 7. row-feet/treatment x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.0) Table 2. Average yield for the Monsanto smartstax refuge in a bag study: Crawfordsville, IA. Bushels/ Treatment 2 Form. Rate 3 Placement Acre,6,7 Smartstax RIB (9%Bt/%non-Bt) Smartstax Pioneer-HXX Pioneer OAM DeKalb-RR2 + Aztec 2.G 0. T-band 3 DeKalb-RR Planted May, 20; machine harvested October 7, 20 2 Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer OAM = Pioneer Optimum AcreMax (Pioneer P028XR); Smartstax (9% Bt corn/% non-bt corn) = DeKalb brand Smartstax Refuge in a bag (DKC-08JRM); Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer P028AMI); DeKalb-RR2 = DeKalb brand RR (DKC -08) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on observation (-row treatment x 9 row-feet/treatment) 6 No significant differences between means (ANOVA, P < 0.0) 7 Yields converted to % Moisture

27 23 Table 22. Average stand count for the Monsanto genuity smartstax features and benefits study: Crawfordsville, IA. Stand Treatment 2 Form. Rate 3 Placement Counts,6 Smartstax a DeKalb-RR2 + Force 3.0G 0.2 T-band 33.2ab DeKalb-RR ab Pioneer-HXX ab Pioneer-RR2 + Force 3.0G 0.2 T-band 32.00ab Agrisure + Force 3.0G 0.2 T-band 3.7ab Agrisure c Planted May, 20; evaluated June 7 and September 28, 20 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 3F3PDR); Agrisure = Syngenta Agrisure GT hybrid (N3-W2); DeKalb-RR2 = DeKalb brand RR (DKC -08); Agrisure 2 = Syngenta Agrisure 3 hybrid (N3C-3); Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 3F37) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time Means based on 8 observations ( rows/ treatment x 7. row-feet/treatment x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.0) Table 23. Average yield for the Monsanto genuity smartstax features and benefits study: Crawfordsville, IA. Bushels/ Treatment 2 Form. Rate 3 Placement Acre,6,7 Pioneer-HXX DeKalb-RR Smartstax DeKalb-RR2 + Force 3.0G 0.2 T-band 3 Pioneer-RR2 + Force 3.0G 0.2 T-band Agrisure Agrisure + Force 3.0G 0.2 T-band Planted May, 20; machine harvested October 2, 20 2 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 3F3PDR); Agrisure = Syngenta Agrisure GT hybrid (N3-W2); DeKalb-RR2 = DeKalb brand RR (DKC -08); Agrisure 2 = Syngenta Agrisure 3 hybrid (N3C-3); Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 3F37) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on observations (-row treatment x 9 row-feet/treatment) 6 No significant differences between means (ANOVA, P < 0.0) 7 Yields converted to % Moisture

28 2 Table 2. Average root-injury and product consistency for the Monsanto corn rootworm study: Northeast Iowa. Node- Product Treatment 2 Form. Rate 3 Placement Injury,6,7 Consistency 8,9 YGVT3 + Aztec 2.G 0. T-band 0.30a 6a DeKalb-Iso + Aztec 2.G 0. T-band 0.33a a Smartstax a a YGVT3 + Force 3.0G 0.2 T-band 0.7a 3ab DeKalb-Iso + Force 3.0G 0.2 T-band.23 b 3ab My-HXT b 0 c YGVT c bc DeKalb-Iso d 0 c Planted May 3, 20; evaluated August 0, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Chemical and check means based on 20 observations ( roots/2 rows x replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0.2 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 2. Average stand count for the Monsanto corn rootworm study: Northeast Iowa. Stand Treatment 2 Form. Rate 3 Placement Counts,6 DeKalb-Iso + Aztec 2.G 0. T-band 36.0 YGVT3 + Force 3.0G 0.2 T-band 36.2 DeKalb-Iso My-HXT YGVT YGVT3 + Aztec 2.G 0. T-band 3.2 DeKalb-Iso + Force 3.0G 0.2 T-band 3.00 Smartstax Planted May 3, 20; evaluated June 2 and September 6, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time Means based on 6 observations ( row treatment x 7. row-feet/treatment x replications) 6 No significant differences between means (ANOVA, P < 0.0)

29 2 Table 26. Average percent lodging for the Monsanto corn rootworm study: Northeast Iowa. % Treatment 2 Form. Rate 3 Placement Lodging,6 YGVT3 + Force 3.0G 0.2 T-band a DeKalb-Iso + Aztec 2.G 0. T-band 3a Smartstax a DeKalb-Iso + Force 3.0G 0.2 T-band 7a YGVT3 + Aztec 2.G 0. T-band a My-HXT a YGVT b DeKalb-Iso c Planted May 3, 20; evaluated September 6, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on 6 observations (-row treatment x7. row-feet/treatment x replications) 6 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 27. Average yield for the Monsanto corn rootworm study: Northeast Iowa. Bushels/ Treatment 2 Form. Rate 3 Placement Acre,6,7 DeKalb-Iso + Aztec 2.G 0. T-band 23 YGVT3 + Force 3.0G 0.2 T-band 22 YGVT3 + Aztec 2.G 0. T-band 22 My-HXT Smartstax DeKalb-Iso + Force 3.0G 0.2 T-band 203 DeKalb-Iso YGVT Planted May 3, 20; machine harvested October, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on observations (-row treatment x 3 row-feet/treatment x replications) 6 No significant differences between means (ANOVA, P < 0.0) 7 Yields converted to% Moisture 8 Means based on 3 observations (-row treatment x 3 row-feet/treatment x 3 replications)

30 26 Node- Product Treatment 2,3 Form. Rate Placement Injury 6,7,8 Consistency 9,0 DeKalb-Smartstax + Aztec 2.G 0. In-Furrow 0.00a 00a My-HXX + SmartChoice-SB G 0.8 In-Furrow 0.2 b 7ab My-Smartstax bc ab DeKalb-Iso + Aztec 2.G 0. In-Furrow 0.6 cd bc My-HXX + Force 3.0G 0.2 In-Furrow 0.68 cd 3abc My-HXX + Counter-SB 20G 0.90 In-Furrow 0.8 d bc My-9%SSTX/%Non-Bt cd 0ab My-HXX de bc My-Iso + Force 3.0G 0.2 In-Furrow. ef 0 c DeKalb-Iso fg 0 c My-Iso g 0 c Planted May, 20; evaluated August 8, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-feet In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Chemical and check means (except My-9%SSTX/%Non-Bt treatment) based on 20 observations ( roots/2 rows x replications) 7 Iowa State Node-Injury scale (0-3). Number of full or partial nodes completely eaten 8 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 9 Product consistency = Percentage of times nodal injury was 0.2 (¼ node eaten) or less 0 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) For the refuge in the bag treatment (My-9%SSTX/%Non-Bt), mean based on 36 observations (9 roots/2 rows (6 Smartstax (3 adjacent roots and 3 distant roots to a Non-Bt plant) + 3 Non-Bt) x replications) Table 29. Yield study: Nashua, IA. Stand Treatment 2,3 Form. Rate Placement Count 6,7 My-Iso + Force 3.0G 0.2 In-Furrow DeKalb-Iso + Aztec 2.G 0. In-Furrow 3.7 My-HXX + SmartChoice-SB G 0.8 In-Furrow 3.0 My-Smartstax My-HXX + Counter-SB 20G 0.90 In-Furrow 3.2 DeKalb-Smartstax + Aztec 2.G 0. In-Furrow 3.2 My-HXX My-Iso My-HXX + Force 3.0G 0.2 In-Furrow 3.2 DeKalb-Iso My-9%SSTX/%Non-Bt Planted May, 20; evaluated June 7 & September 23, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-feet In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 6 observations (2-row treatment x7. row-feet/treatment x replications x 2 evaluation dates) 7 No significant differences between means (ANOVA, P < 0.0)

31 27 Table 30. Yield study: Nashua, IA. % Treatment 2,3 Form. Rate Placement Lodging 6,7 My-Iso + Force 3.0G 0.2 In-Furrow 0a My-Smartstax a My-9%SSTX/%Non-Bt a My-HXX + Force 3.0G 0.2 In-Furrow 0a My-HXX a My-HXX + Counter-SB 20G 0.90 In-Furrow 0a My-HXX + SmartChoice-SB G 0.8 In-Furrow 0a DeKalb-Iso + Aztec 2.G 0. In-Furrow 0a DeKalb-Smartstax + Aztec 2.G 0. In-Furrow a My-Iso b DeKalb-Iso b Planted May, 20; evaluated September 23, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-feet In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on 8 observations (2-row treatment x7. row-feet/treatment x replications) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 3. Yield study: Nashua, IA. Bushels/ Treatment 2,3 Form. Rate Placement Acre 6,7,8 DeKalb-Iso + Aztec 2.G 0. In-Furrow 22a My-HXX + SmartChoice-SB G 0.8 In-Furrow 207a My-Smartstax a My-HXX + Force 3.0G 0.2 In-Furrow 200a My-HXX + Counter-SB 20G 0.90 In-Furrow 200a My-HXX a DeKalb-Smartstax + Aztec 2.G 0. In-Furrow 97a My-Iso + Force 3.0G 0.2 In-Furrow 97a My-9%SSTX/%Non-Bt a My-Iso b DeKalb-Iso b Planted May, 20; machine harvested October, 20 2 My-Smartstax = Mycogen Smartstax (Mycogen 2K9); My-HXX = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb- Smartstax = DeKalb Smartstax (DKC6-2); DeKalb-Iso = DeKalb brand RR Isoline (DKC 6-72); My-Iso = Mycogen brand RR2 (Mycogen 2K9); My-9%SSTX/%Non-Bt = Mycogen 9% Smartstax + % Non-Bt (Refuge in a Bag) (Mycogen 2K9+ Mycogen 2K9) 3 My-Iso (Mycogen 2K9) is the isoline of My-HXX (Mycogen 2K92) Insecticide listed as ounces a.i. per,000 row-ft In-Furrow = insecticide applied at planting time; SB = SmartBox application at planting time 6 Means based on observations (2-row treatment x 69 row-feet/treatment x replications) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Yields converted to% Moisture

32 28 Table 32. Average root-injury and product consistency for the Monsanto corn rootworm study: Nashua, IA. Node- Product Treatment 2 Form. Rate 3 Placement Injury,6,7 Consistency 8,9 Smartstax a 90a YGVT3 + Aztec 2.G 0. T-band 0.32 b b YGVT3 + Force 3.0G 0.2 T-band 0.3 bc 0 b My-HXT bc bc DeKalb-Iso + Aztec 2.G 0. T-band 0.62 c 20 bc DeKalb-Iso + Force 3.0G 0.2 T-band 0.6 c c YGVT d c DeKalb-Iso e 0 c Planted May 3, 20; evaluated August 0, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Chemical and check means based on 20 observations ( roots/2 rows x replications) 6 Iowa State Node-Injury Scale (0-3). Number of full or partial nodes completely eaten 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0.2 (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 33. Average stand count for the Monsanto corn rootworm study: Nashua, IA. Stand Treatment 2 Form. Rate 3 Placement Counts,6 DeKalb-Iso + Force 3.0G 0.2 T-band 3.7a My-HXT a YGVT ab DeKalb-Iso ab YGVT3 + Aztec 2.G 0. T-band 3.00ab DeKalb-Iso + Aztec 2.G 0. T-band 3.7ab YGVT3 + Force 3.0G 0.2 T-band 3.2ab Smartstax b Planted May 3, 20; evaluated June 2 and September 6, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time Means based on 32 observations ( row treatment x 7. row-feet/treatment x replications x 2 evaluations) 6 No significant differences between means (ANOVA, P < 0.0)

33 29 Table 3. Average percent lodging for the Monsanto corn rootworm study: Nashua, IA. % Treatment 2 Form. Rate 3 Placement Lodging,6 DeKalb-Iso + Aztec 2.G 0. T-band 0a DeKalb-Iso + Force 3.0G 0.2 T-band 0a Smartstax a My-HXT a YGVT3 + Aztec 2.G 0. T-band 0a YGVT3 + Force 3.0G 0.2 T-band 0a YGVT a DeKalb-Iso b Planted May 3, 20; evaluated September 6, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); YGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on6 observations (-row treatment x7. row-feet/treatment x replications) 6 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 3. Average yield for the Monsanto corn rootworm study: Nashua, IA. Bushels/ Treatment 2 Form. Rate 3 Placement Acre,6,7 My-HXT a YGVT3 + Force 3.0G 0.2 T-band 20 b Smartstax b YGVT bc YGVT3 + Aztec 2.G 0. T-band 20 bc DeKalb-Iso + Force 3.0G 0.2 T-band 20 bc DeKalb-Iso c DeKalb-Iso + Aztec 2.G 0. T-band 82 c Planted May 3, 20; machine harvested October, 20 2 My-HXT = Mycogen brand Herculex XTRA (Mycogen 2K92); DeKalb-Iso = DeKalb brand RR (DKC -92); VGVT3 = YieldGard VT Triple (DKC6-60); Smartstax = DeKalb brand (DKC6-6) 3 Insecticide listed as ounces a.i. per,000 row-feet T-band = insecticide applied at planting time Means based on observations (-row treatment x 3 row-feet/treatment x replications) 6 No significant differences between means (ANOVA, P < 0.0) 7 Yields converted to % Moisture

34 30 Table 36. Average stand count for the Pioneer ECB Optimum AcreMax study: Ames, IA. Stand Treatment 2,3 Count, HX x YGCB (with % blended refuge plants) F 68.2 HX x YGCB HX x YGCB (with 0% blended refuge plants) HX 67.7 RR Planted May 0, 20; evaluated June 7, 20 2 HX = Herculex trait (Pioneer hybrid P06HR); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P06R); HX x YGCB = Pyramid Hybrid (Pioneer P06YHR/P06R); 3 HX = Herculex trait. Herculex Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty Link (LL), Ignite and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard Corn Borer gene. YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company. Means based on 6 observations ( row treatment x 38 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Table 37. Average whorl injury for the Pioneer ECB Optimum AcreMax study: Ames, IA. ECB whorl Treatment 2,3 Injury, HX (Pure stand) 6 F 9.0a HX x YGCB (Pure stand) 6 9.0a HX x YGCB (pyramid with % blended refuge plants) 9 9.0a RR2 (% blended refuge) 8 9.0a HX x YGCB (pyramid with 0% blended refuge plants) 9 8.9a RR2 (Pure stand) 7 8.9a RR2 (0% blended refuge) b Planted May 0, 20; evaluated July, 20 2 HX = Herculex trait (Pioneer hybrid P06HR); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P06R); HX x YGCB = Pyramid Hybrid (Pioneer P06YHR/P06R); 3 HX = Herculex trait. Herculex Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty Link (LL), Ignite and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard Corn Borer gene. YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company. Means based on the Pioneer 9- rating scale for evaluating first generation ECB whorl injury (ECBLF). Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 6 For the pure stand treatments of HX x YGCB, 8 Bt plants were evaluated (2 plants/2 rows x replications) 7 For the pure stand treatment of RR2, 8 non-bt roots were evaluated (2 plants/2 rows x replications) 8 For the RR2 blended refuge treatments (% & 0%), 2 non-bt plants were evaluated (6 plants/2 rows x replications) 9 For the pyramided treatments (90% & 9%), 8 Bt plants were evaluated (2 plants/2 rows x replications)

35 3 Table 38. Average tunneling (cm) for the Pioneer ECB Optimum AcreMax study: Ames, IA. Average ECB Treatment 2,3 Tunneling (cm) HX x YGCB (Pure stand) 0.00a HX (Pure stand) 0.07a HX x YGCB (pyramid with % blended refuge plants) 8 0.3a HX x YGCB (pyramid with 0% blended refuge plants) 8 0.3a RR2 (% blended refuge) b RR2 (0% blended refuge) 7.92 bc RR2 (Pure stand) c Planted May 0, 20; evaluated October 6 & 7, 20 2 HX = Herculex trait (Pioneer hybrid P06HR); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P06R); HX x YGCB = Pyramid Hybrid (Pioneer P06YHR/P06R); 3 HX = Herculex trait. Herculex Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Liberty Link (LL), Ignite and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard Corn Borer gene. YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company. Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) For the pure stand treatments of HX x YGCB, 8 Bt plants were evaluated (2 plants/2 rows x replications) 6 For the pure stand treatment of RR2, 8 non-bt roots were evaluated (2 plants/2 rows x replications) 7 For the RR2 blended refuge treatments (% & 0%), 2 non-bt plants were evaluated (6 plants/2 rows x replications) 8 For the pyramided treatments (90% & 9%), 8 Bt plants were evaluated (2 plants/2 rows x replications) Table 39. Average yield for the Pioneer ECB Optimum AcreMax study: Ames, IA. Treatment 2,3 Yield,,6 HX x YGCB 2 HX 209 HX x YGCB with % blended refuge (RR2) 209 RR2 208 HX x YGCB with 0% blended refuge (RR2) 202 Planted May 0, 20; machine harvested October 3, HX = Herculex trait (Pioneer hybrid P06HR); RR2 = Roundup Ready Corn 2 trait (Pioneer hybrid P06R); HX x YGCB = Pyramid Hybrid (Pioneer P06YHR/P06R); 3 HX = Herculex trait. Herculex Insect Protection technology by Dow AgroSciences LLC and Pioneer Hi-Bred. Ignite and the Water Droplet logo are registered trademarks of Bayer. YGCB = YieldGard Corn Borer gene. YieldGard and the YieldGard Corn Borer design are registered trademarks of Monsanto Company. RR2 = Roundup Ready Corn 2 gene. Roundup Ready is a registered trademark of Monsanto Company. Means based on observations (2-rows x 38 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) 6 Yields converted to% Moisture

36 32 Table 0. Stand counts for the Dow AgroSciences Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA. Stand Treatment 2 Counts 3, RR2 3.0a Smartstax 30.0ab HXX 28.0 b Planted May 0, 20; stand counts evaluated on June 7, 20 2 RR2 = Mycogen 2K9; HXX = Mycogen Herculex XTRA 2K92; Smartstax = Mycogen 2K9 3 Stand counts based on 8 observations (2 rows/7. feet of row x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table. Total kernels, damaged kernels, and percent damaged for the Dow AgroSciences Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA.,2 Total Damaged % Treatment 3 Kernels/Ear Kernels/Ear, Damaged/Ear 6,7 Smartstax 9.23a 0.2a HXX b 0.73 b RR b 0.96 b Planted May 0, 20; evaluated August 8, 20 2 Ten corn ears per row in rows 2 and 3 of each plot were infested on August, 20 with st instar CEW larvae 3 RR2 = Mycogen 2K9; HXX = Mycogen Herculex XTRA 2K92; Smartstax = Mycogen 2K9 Mean total kernels, damaged kernels counts and % damaged based on 0 observations (0 ears/treatment x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 6 Mean total kernels, damaged kernels counts and % damaged based on 0 observations (0 ears/treatment x replications) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 2. Average kernel area (cm 2 ) consumed and number of total damaged ears for the Dow AgroSciences Smartstax vs Herculex XTRA and RR2 corn study, Ames, IA.,2 Kernel Area Total Treatment 3 Consumed (cm 2 ), Damaged Ears 6,7 Smartstax 0.2a 3a HXX.8 b 2 b RR2.6 b 26 b Planted May 0, 20; evaluated August 8, 20 2 Ten corn ears per row in rows 2 and 3 of each plot were infested on August, 20 with st instar CEW larvae 3 RR2 = Mycogen 2K9; HXX = Mycogen Herculex XTRA 2K92; Smartstax = Mycogen 2K9 Kernel area consumed based on 0 observations (0 ears/treatment x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 6 Total damaged ears based on 0 observations (0 ears/treatment x replications) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

37 33 Table 3. Total kernels, damaged kernels, and percent damaged for the Monsanto genuity smartstax features and benefits study, Ames, IA.,2 Total Damaged Percent Treatment 3 Form. Rate Placement Kernels/Ear 6 Kernels/Ear 6,7 Damaged/Ear 8,9 Agrisure a 0.0a Smartstax a 0.0a Pioneer-HXX b.0 b DeKalb-RR b 2. b DeKalb-RR2 + Force 2.G 0.2 T-band 38.8 b 3.7 b Agrisure + Force 2.G 0.2 T-band b 3. b Pioneer-RR2 + Force 2.G 0.2 T-band 3.2 b 3. b Planted May 0, 20; evaluated August 2, 20 2 Ten corn ears per row in rows 2 and 7 of each plot were infested on August 0, 20 with 2 nd instar CEW larvae 3 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 3F3PDR); Agrisure = Syngenta Agrisure GT hybrid (N3-W2); DeKalb-RR2 = DeKalb brand RR (DKC -08); Agrisure 2 = Syngenta Agrisure 3 hybrid (N3C-3); Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 3F37) Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time 6 Mean total kernels, damaged kernels counts and % damaged based on 0 observations (0 ears/treatment x replications) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Mean total kernels, damaged kernels counts and % damaged based on 0 observations (0 ears/treatment x replications) 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table. Kernel area (cm 2 ) consumed and number of total damaged ears for the Monsanto genuity smartstax features and benefits study, Ames, IA.,2 Kernel Area Total Treatment 3 Form. Rate Placement Consumed (cm 2 ) 6,7 Damaged Ears 8,9 Agrisure a 0a Smartstax a 0a Pioneer-HXX b 8 b DeKalb-RR b 9 b DeKalb-RR2 + Force 2.G 0.2 T-band.97 b 7 b Agrisure + Force 2.G 0.2 T-band.0 b 7 b Pioneer-RR2 + Force 2.G 0.2 T-band.0 b 0 b Planted May 0, 20; evaluated August 2, 20 2 Ten corn ears per row in rows 2 and 7 of each plot were infested on August 0, 20 with 2 nd instar CEW larvae 3 Pioneer-HXX = Pioneer brand Herculex XTRA (Pioneer 3F3PDR); Agrisure = Syngenta Agrisure GT hybrid (N3-W2); DeKalb-RR2 = DeKalb brand RR (DKC -08); Agrisure 2 = Syngenta Agrisure 3 hybrid (N3C-3); Smartstax = DeKalb brand Smartstax (DKC -09); Pioneer-RR2 = Pioneer brand RR2 (Pioneer 3F37) Insecticide listed as ounces a.i. per,000 row-feet T-band = Insecticide applied at planting time 6 Kernel area consumed based on 0 observations (0 ears/treatment x replications) 7 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Total damaged ears based on 0 observations (0 ears/treatment x replications) 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

38 3 APPENDIX I Agronomic Information

39 3 20 Field History Data Ames, IA Corn rootworm studies (following trap crop) Crawfordsville, IA Corn rootworm studies (following trap crop) Insecticide History 200 No Insecticide (trap crop) No Insecticide (trap crop) 2009 No Insecticide (soybeans) Insecticide Test Plot 2008 Insecticide Test Plot No Insecticide (trap crop) 2007 No Insecticide (trap crop) Insecticide Test Plot Tillage Variety Fall-disk ripper; Spring-field cultivation (2X) Pioneer P39XR, Pioneer TQ3882, Pioneer P39HR, Pioneer P03R, DKC6-60, DKC-92, DKC6-6, Mycogen 2K92, DKC6-36, DKC6-2, DKC62-97DKL, DKC6-2JRM, DKC6-97WTM, DKC62-97BPY Fall-chiseled; Spring-field cultivated DKC6-2 & DKC6-72, Mycogen 2K9, Mycogen 2K92, Mycogen 2K9, Pioneer 3F3PDR, Agrisure N3C-3, DKC-09, DKC-08, Agrisure N3-W2, Pioneer 3F37, DKC-08 Planting Date(s) May 0 May Planting Rate 3,600 seeds/a 3,600 seeds/a Herbicide 6 oz Balance + 32 oz Glystar-May 2; 3 oz Capreno-June 8 2. qt Harness Extra + 3 oz Balance Flexx-May 7; 22 oz Roundup PowerMax +.2 qt Infantry L + 2. lbs AMS-June 7 Fertilizer 2 N P K N P K Fall Applied Preplant Dates Stand Count June 7, September 22 June 8, September 30 Root Digging August 0,,2 August 9 Lodging September 2,22 September 30 Harvest October 2,3 October 7 Soil Type Clay Loam Silty Clay Loam Soil Organic Matter % 3.7. Soil ph.6.9 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-Preplant: 36 lbs actual nitrogen applied as urea on May 2 (Ames); Spring-Preplant 20 lbs actual nitrogen applied as anhydrous ammonia ( ) on April 2 (Crawfordsville).

40 36 20 Field History Data Nashua, IA Corn rootworm studies (following trap crop) Nashua, IA Monsanto corn rootworm study (following continuous corn) Insecticide History 200 No Insecticide (trap crop) VT3 Hybrid Test Plot (Aztec) 2009 Insecticide Test Plot VT3 Hybrid Test Plot 2008 No Insecticide (trap crop) VT3 Hybrid Test Plot 2007 Insecticide Test Plot YIELDGARD + Hybrid Test Plot Tillage Variety Fall-chopped stalks & chisel; Spring-field cultivated DKC6-2 & DKC6-72, Mycogen 2K9, Mycogen 2K92, Mycogen 2K9, Fall-chopped stalks & chisel; Springfield cultivated DKC6-60, DKC-92 & DKC6-6; Mycogen 2K92 Planting Date(s) May May Planting Rate 3,600 seeds/a 3,600 seeds/a Herbicides 2.3 qt Harness Extra-May 9; 2 pt Marksman-June 2.3 qt Harness Extra-May 9; 2 pt Marksman-June Fertilizer 2 N P K N P K Fall Applied Preplant Dates Stand Count June 7 & September 23 June 7 & September 23 Root Digging August August Lodging September 23 September 23 Harvest October October Soil Type Loam Loam Soil Organic Matter % Soil ph Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 200 lbs actual nitrogen applied as anhydrous ammonia ( ) on April 3 (Nashua).

41 37 20 Field History Data Northeast Iowa Monsanto corn rootworm study (following continuous corn) Ames, IA Pioneer ECB OAM II study (following soybeans) Insecticide History 200 Corn No insecticide (SB s) 2009 Corn (Insecticide applied) No insecticide (SB s) 2008 Corn (Insecticide applied) No insecticide (SB s) 2007 Corn (Insecticide applied) Soybeans (with Warrior) Tillage Variety Fall-chopped stalks & chisel; Spring-field cultivated DKC6-60, DKC-92 & DKC6-6; Mycogen 2K92 Spring-field cultivation (2X) Pioneer P06HR, Pioneer P06YHR, Pioneer P06R Planting Date May May Planting Rate 3,600 seeds/a 3,600 seeds/a Herbicides 2. pts G-Max Lite TM + qt Accomplish-April 6; 22 oz Roundup PowerMax + oz Status + 2 oz Radiate-June 6 Fungicides oz Headline-June 6; 9 oz Headline Amp-July pts Harness-May 2; 3 oz Capreno-June 8. None Fertilizer 2 N P K N P K Fall Applied Preplant Dates Stand Count June 2 & September 6 June 7, & 30, September 2 Root Digging August N/A ECB Scoring - N/A July Stalk Rating N/A October 6,7 Lodging September 6 September 2 Harvest October October 2 Soil Type Clay loam Loam Soil Organic Matter 3.. Soil ph 6..8 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 36 lbs actual nitrogen (urea) applied on May 2 (Ames). Fall- 200 applied 60 lbs of Phosphorus and 90 lbs of potassium (Ames). Spring-preplant 7 lbs applied as 28% Nitrogen fertilizer on April 6 (Northeast Iowa). Fall-applied 70 lbs actual nitrogen applied as anhydrous ammonia ( ) in November 200 (Northeast Iowa).

42 38 20 Field History Data Ames, IA-Old Dairy Farm Syngenta efficacy study (following corn) Ames, IA-South Dairy Farm Syngenta efficacy study (following corn) Insecticide History 200 Corn Corn 2009 Soybeans Corn 2008 Corn Soybeans (Warrior) 2007 Soybeans Corn Tillage Fall-Chisel plow Spring-field cultivation (2X) Fall-Disk ripper Spring-field cultivation (2X) Variety Agrisure GT/CB/LL, Agrisure 3000GT, Agrisure Viptera 3, Agrisure Viptera 322, Agrisure 322 Viptera EZ Refuge, Agrisure 322 Viptera EZ Refuge2 Agrisure GT/CB/LL, Agrisure 3000GT, Agrisure Viptera 3, Agrisure Viptera 322, Agrisure 322 Viptera EZ Refuge, Agrisure 322 Viptera EZ Refuge2 Planting Date May 8 May 9 Planting Rate 32,000 seeds/a 32,000 seeds/a Herbicides 28 oz Dual II Magnum-May ; 3 oz Laudis-June oz Dual II Magnum-May 9; 6 oz Status-June 2. Fertilizer 2 N P K N P K Fall Applied Preplant Dates Stand Count June 8 & October 9 June 8 & October 20 Root Digging August 7 August 7 Lodging October 9 October 20 Harvest October 9 October 20 Soil Type Loam Loam Soil Organic Matter % Soil ph Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 20 lbs actual nitrogen (urea) applied on May 0 at Old Dairy Farm (Ames). Spring-preplant 20 lbs actual nitrogen (urea) applied on May 6 at South Dairy Farm (Ames).

43 39 20 Field History Data Ames, IA Dow AgroSciences CEW study (following soybeans) Ames, IA Monsanto features & benefits study (following trap crop) Insecticide History 200 No insecticide (SB s) No Insecticide (trap crop) 2009 No insecticide (corn) No Insecticide (soybeans) 2008 No insecticide (SB s) Insecticide Test Plot 2007 No insecticide (corn) No Insecticide (trap crop) Tillage Spring-field cultivation (2X) Fall-disk ripper; Spring-field cultivation (2X) Variety Mycogen 2K9, Mycogen 2K92, Mycogen 2K9 Pioneer 3F3PDR, Agrisure N3C- 3, DKC-09, DKC-08, Agrisure N3-W2, Pioneer 3F37, DKC-08 Planting Date May 0 May 0 Planting Rate 3,600 seeds/a 3,600 seeds/a Herbicides 2. pts Harness-May 2; 3 oz Capreno-June 8. 6 oz Balance + 32 oz Glystar-May 2; 3 oz Capreno-June 8 Fertilizer 2 N P K N P K Fall Applied Preplant Dates Stand Count June 7 June 7 and September 22 CEW Infestations August August 0 CEW Ear assessments August 8 August 2 Soil Type Loam Clay Loam Soil Organic Matter. 3.7 Soil ph.8.6 Expressed as formulation per acre. 2 Expressed as pounds per acre. Spring-preplant 36 lbs actual nitrogen (urea) applied on May 2. (Ames); Fall-200 applied 60 lbs of Phosphorus and 90 lbs of potassium. (Ames-Dow Agro Sciences study only).

44 0 APPENDIX II Weather Data

45 Ames Rainfall and Temperature 20 May June July Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches Mean/Total Normal D.F.N Weather station located miles WSW of test site.

46 2 Ames Rainfall and Temperature 20 August September October Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches Mean/Total Normal D.F.N Weather station located miles WSW of test site.

47 3 Crawfordsville Rainfall and Temperature 20 May June July Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches Mean/Total Normal D.F.N Weather station located at test site.

48 Crawfordsville Rainfall and Temperature 20 August September October Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches Mean/Total Normal D.F.N Weather station located at test site.

49 Northeast Iowa Rainfall and Temperature 20 May June July Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches T T T T T T T 8 6 T T T T T T T T T Mean/Total Normal D.F.N Closest weather station located in Manchester, Iowa, approximately 20 miles West Northwest.

50 6 Northeast Iowa Rainfall and Temperature 20 August September October Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches T T T 66 T T T T T T Mean/Total Normal D.F.N Closest weather station located in Manchester, Iowa, approximately 20 miles West Northwest.

51 7 Nashua Rainfall and Temperature 20 May June July Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches T T T T T T T Mean/Total Normal D.F.N Weather station located at test site.

52 8 Nashua Rainfall and Temperature 20 August September October Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day High Low Inches High Low Inches High Low Inches T T T T Mean/Total Normal D.F.N Weather station located at test site.

53 9 APPENDIX III Materials Tested

54 0 Materials Tested in 20 Iowa State University Efficacy Studies Common/code name Formulation Chemical name Company Aztec 2.G tebupirimphos & cyfluthrin AMVAC Chem. Corp. Counter 20G terbufos AMVAC Chem. Corp. Cruiser Commercially applied seed trt (0.2 mg/sd) thiamethoxam Syngenta Cruiser Extreme 20 Commercially applied seed trt (0.2 mg/sd) thiamethoxam+ three fungicides (Maxim XL+Apron XL and Dynasty) Syngenta DKC-92 RR2 + commercially applied Poncho 00/VOTIVO Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto DKC6-60 (YieldGard VT Triple with commercial seed trt) DKC6-6 (Smartstax with commercial seed trt) DKC-08 DKC-09 (Smartstax with commercial seed trt) DKC6-2 (Smartstax with commercial seed trt) DKC6-2JRM (Smartstax RIB (9%Bt/%non-Bt) with commercial seed trt) DKC6-36 DKC6-72 DKC6-97DKL VT3P RIB (9%Bt/%non-Bt) with commercial seed trt Transgenic seedcorn (YGRW+YGCB+RR2) + commercially applied Poncho 00/VOTIVO Transgenic seedcorn (CRW/RR2(VT)) +BT (VT)+CRW (HXRW)+BT (HX) commercially applied Poncho 00/VOTIVO RR2 + commercially applied Poncho 00/VOTIVO Transgenic seedcorn (CRW/RR2(VT)) +BT (VT)+CRW (HXRW)+BT (HX) commercially applied Poncho 00/VOTIVO Transgenic seedcorn (CRW/RR2(VT)) +BT (VT)+CRW (HXRW)+BT (HX) commercially applied Poncho 00/VOTIVO Transgenic seedcorn (CRW/RR2(VT)) +BT (VT)+CRW (HXRW)+BT (HX) commercially applied Poncho 00/VOTIVO RR2 + commercially applied Poncho 00/VOTIVO DKC6-72 (RR2) true isoline seed of DKC commercially applied Poncho 00/VOTIVO Transgenic seedcorn (YGRW+YGCB+RR2)+ commercially applied Poncho 00/VOTIVO Cry3Bb (MON 863) + CryAb (MON80) + Roundup Ready Corn 2 + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Cry3Bb & RR2 (MON 8807) + CryA.0&Cry2Ab2(MON8903) +Cry3Ab&Cry3Ab(DAS922) +CryF&LL(TC07) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.2 mg/seed) + Bacillus firmus I-82 Monsanto Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Cry3Bb & RR2 (MON 8807) + CryA.0&Cry2Ab2(MON8903) +Cry3Ab&Cry3Ab(DAS922) +CryF&LL(TC07) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Cry3Bb & RR2 (MON 8807) + CryA.0&Cry2Ab2(MON8903) +Cry3Ab&Cry3Ab(DAS922) +CryF&LL(TC07) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Cry3Bb & RR2 (MON 8807) + CryA.0&Cry2Ab2(MON8903) +Cry3Ab&Cry3Ab(DAS922) +CryF&LL(TC07) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto Cry3Bb (MON 863) + CryA.0/Cry2Ab2 (MON8903) + Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto

55 Materials Tested for 20 (Continued) Common/code name Formulation Chemical name Company DKC6-97WTM VT3P RIB (00%Bt) with commercial seed trt Transgenic seedcorn (YGRW+YGCB) + commercially applied Poncho 00/VOTIVO Cry3Bb (MON 863) + CryA.0/Cry2Ab2 (MON8903) + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Monsanto DKC6-97BPY VT3P RIB (80%Bt/20%non-Bt) with commercial seed trt Transgenic seedcorn (YGRW+YGCB+RR2)+ commercially applied Poncho 00/VOTIVO Force 3G tefluthrin Mycogen 2K9 true isoline seed of Mycogen 2K9 Mycogen 2K92 Mycogen 2K92 (Herculex/RR) with Cruiser Extreme 20 seed trt Dow Background Mycogen 2K9 (Smartstax) with Cruiser Extreme 20 seed trt Mycogen 2K9+2K9 (RIB) with Cruiser Extreme 20 seed trt Pioneer P03R (RR2) with Poncho 20/VOTIVO seed treatment Pioneer P03R (RR2) with Cruiser 20 seed treatment Dow Background Dow Background Pioneer Background Pioneer Background Pioneer TQ3882 (pyramid) with Cruiser 20 seed treatment Pioneer Background Pioneer P39HR (HX) with Cruiser 20 seed treatment Pioneer P39XR (Herculex XTRA) with Cruiser 20 seed treatment Pioneer P06HR (HX) with Cruiser 20 seed treatment Pioneer P39HR near isoline seed of Pioneer P39XR Pioneer Background Pioneer P06HR near isoline seed of Pioneer P06R Cry3Bb (MON 863) + CryA.0/Cry2Ab2 (MON8903) + Roundup Ready 2 Corn + fludioxonil & mefenoxam fungicides + clothianidin seed trt (0.0 mg/seed) + Bacillus firmus I-82 Roundup Ready 2 Corn + Thiamethoxam seed trt (0.2 mg/seed) Cry3AB & Cry3Ab + CryF + RR2 + thiamethoxam seed trt (0.2 mg/seed) Cry3AB & Cry3Ab + CryF + Cry38b + CryA.0 + Cry 2Ab2 + RR2 + Liberty Link + thiamethoxam seed trt (0.2 mg/seed) Cry3AB & Cry3Ab + CryF + Cry38b + CryA.0 + Cry 2Ab2 + RR2 + Liberty Link + thiamethoxam seed trt (0.2 mg/seed) Roundup Ready Corn 2 + mefenoxam fungicide + clothianidin seed trt (.2 mg/seed) + Bacillus firmus I-82 Roundup Ready 2 Corn + mefenoxam fungicide + thiamethoxam seed trt (0.2 mg/seed) Cry3Ab & Cry3Ab + CryF + CryAb + MIR60 + mefenoxam fungicide + thiamethoxam seed trt (0.2 mg/seed) mefenoxam fungicide + Clothianidin + thiamethoxam seed trt (0.2 mg/seed) Cry3AB & Cry3Ab + CryF + mefenoxam fungicide + thiamethoxam seed trt (0.2 mg/seed) mefenoxam fungicide + Clothianidin + thiamethoxam seed trt (0.2 mg/seed) Monsanto Syngenta Crop Protection Dow AgroSciences Dow AgroSciences Dow Background Dow Background Pioneer Hi-Bred International, Inc Pioneer Hi-Bred International, Inc Pioneer Hi-Bred International, Inc Pioneer Hi-Bred International, Inc Pioneer Hi-Bred International, Inc. Pioneer Hi-Bred International, Inc

56 2 Materials Tested for 20 (Continued) Common/code name Formulation Chemical Name Company Pioneer P06R (RR2) with Cruiser 20 seed treatment Pioneer Background Roundup Ready 2 Corn + mefenoxam fungicide + thiamethoxam seed trt (0.2 mg/seed) Pioneer 3F3PDR (Herculex XTRA) with Cruiser 20 seed treatment Pioneer 3F37 (RR2) with Cruiser 20 seed treatment Poncho 20 Poncho 00 Poncho 20 Pioneer Background Cry3AB & Cry3Ab + CryF + mefenoxam fungicide + thiamethoxam seed trt (0.2 mg/seed) Roundup Ready 2 Corn + mefenoxam fungicide + thiamethoxam seed trt (0.2 mg/seed) Pioneer Hi-Bred International, Inc. Pioneer Hi-Bred International, Inc. Pioneer Hi-Bred Pioneer Background International, Inc Commercially applied seed trt (0.2 mg/seed) clothianidin Bayer CropScience Commercially applied seed trt (0.0 mg/seed) clothianidin Bayer CropScience Commercially applied seed trt (.2 mg/seed) clothianidin Bayer CropScience SmartChoice G Chlorethoxyfos Syngenta Agrisure 3 hybrid (N3C-3) Transgenic seedcorn Bt+MIR60+GA2 Syngenta Agrisure GT hybrid (N3-W2) GA2 mepsps (GA2) CryAb (Bt) + mcry3a (MIR60) + mepsps (GA2) AMVAC Chem. Corp. Syngenta Crop Protection Syngenta Crop Protection

57 3 APPENDIX IV Research Pictures

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