RELIANCE HIGH THROUGHPUT AUTOSAMPLER

Transcription

1 RELIANCE HIGH THROUGHPUT AUTOSAMPLER

2 PHILOSOPHY WHEN CHOOSING AN AUTOSAMPLER, PHARMACEUTICAL LAB MANAGERS ASK FOR HIGH THROUGHPUT, HIGH INJECTION SPEED AND SHORTER PROCESS TIMES. THESE ARE ONLY A BENEFIT IN COMBINATION WITH A MINIMUM OF CARRY-OVER AND TROUBLE-FREE OPERATION OF THE INSTRUMENT. FOR OVER TWO DECADES, SPARK HAS BEEN ACTIVELY ENGAGED IN THE DEVELOPMENT OF LEADING TECHNOLOGY USED IN AUTOMATION OF SAMPLE HANDLING AND SAMPLE INTRODUCTION. TODAY, SPARK INTRODUCES THE FIRST TRULY TEMPERATURE-CONDITIONED HIGH THROUGHPUT AUTOSAMPLER THAT WILL CHANGE THE WAY PEOPLE IMPLEMENT AUTOMATED SAMPLE HANDLING IN THEIR LAB: RELIANCE.

3 RELIANCE The introduction of modern fast detection and data acquisition methods has caused a significant increase in the number of samples processed in laboratories. In contrast, labor resources and process time frames are severely under pressure. Speed, Quality and Reliability of the installed instrumentation are therefore more important than ever. 5th generation autosampler The experiences gained from 15,000 installed units formed the input for our ambition to develop a new autosampler that would satisfy the most stringent requirements for accurate analytical results in a high throughput LC-MS environment. The Reliance High Throughput conditioned autosampler offers maximum throughput and flexibility in sample introduction. The highest quality and reliability standards are met. Reliance features include: A new high-speed injection mode reducing cycle time by more than 50% for a partial loopfill injection including needle wash. Cycle time including wash is less than 35 seconds. Superior precision and market leading carry-over performance. Standard provisions for reducing carry-over of difficult compounds. New high-capacity stacker for a maximum of 9,216 samples. Integrated cooling in both stacker and autosampler guarantees a constant temperature for stacked and processed samples. User-selectable viscosity levels for optimization of cycle time and performance. Programmed and user-definable templates allow use of all commercially available (micro) well plates. Modular design for easy integration in a customized system. Symbiosis and XLC-MS ready. Improved user interface includes most MS data packages support.

4 HIGH THROUGHPUT CAPABILITY High throughput capability is not only determined by the batch size of the autosampler. It is also influenced by instrument downtime and the degree of integration with other peripherals such as LC Pumps and Mass Spectrometers. Reliable and service-friendly The Reliance is designed to withstand the toughest demands, those of the contract laboratory, with on-time analytical results as the prevailing rule. With 24-hour operation all mechanical parts are put to the most severe life cycle test program. Set up a weekend run and rest assured it will inject, time and again, reliably. A special service mode enables a service engineer to check an operations log file. The lifetime records of switching valves, sample tray, dispenser etc. allow for effective preventive maintenance of the unit, assuring its trouble-free functioning. Furthermore, the modular design of electronics and mechanics simplifies replacement and reduces cost and downtime. Intelligent master, intelligent slave For integration with peripheral instruments like LC-pumps and MS detectors, the Reliance control software offers links with all MS data packages. And for those requiring a true front-end sample prep solution, the Reliance fits perfectly into Spark's novel front-end automated sample handling system Symbiosis.

5 INJECTION PRECISION WITH MINIMAL CARRY-OVER Bio-compatibility and Multi Wash Solvent Selection A special Bio-needle is provided for trouble-free processing of biological samples such as plasma or urine. In order to create a metal free sampling path, a PEEK injection valve and PEEK sample needles are available, making the Reliance the perfect autosampler for bioanalysis. A standard multisolvent selection valve allows a choice of up to 3 different wash solvents. Highly sensitive LC-MS/MS systems and increasingly stringent FDA directives, make the elimination of carry-over a most challenging factor in assay development. Spark s proven injection concept and thorough needle wash routines are designed to meet this challenge. Pressure Assisted Sample Aspiration (PASA ), a dual concentric needle system and inside plus outside needle wash make the favorable combination of highest precision and lowest carry-over.

6 The Reliance is the first autosampler with a unique storage capacity of 9216 samples in a closed and conditioned stacker, avoiding condensation and ensuring a constant temperature. A transport feeder automatically picks up randomly accessible well plates from the stacker for processing in the autosampler. During processing, a large capacity Peltier cooling unit maintains internal conditions until the sample is injected. For fully automated online sample preparation and injection, the Reliance is ready to be integrated in Spark s newly developed XLC (extraction Liquid Chromatography)- MS system: Symbiosis. Symbiosis offers high throughput autosampling and online extraction with full LC functionality, truly automating SPE-LC-MS. COOL SAMPLE PREPARATION CAPABILITIES The stability of samples is vital for a reliable analytical result, and loss of sample batches due to degradation frustrate the operator and lab manager. Many of today s autosamplers offer sample cooling options. However, in a high throughput environment with large numbers of biological samples, cooling and conditioning performance must meet the highest standards.

7 VERSATILITY AND CUSTOMIZATION Highly versatile reagent addition and mixing routines will automate complex pre-column derivatizations, dilutions, ph changes, etc. In addition, the programmable sampling height allows aspiration from two-phase samples such as liquid-liquid extracts or samples containing a precipitate. With these capabilities, the Reliance is the standard autosampler for Spark's front-end automated sample handling system Symbiosis. Spark s Reliance is offered as a stand-alone unit, but can also be customized to fit an OEM customer s HPLC system. For OEM applications, Spark offers design and software services to transform the autosampler into the desired system look with a corresponding software interface.

8 HIGH THROUGHPUT AUTOSAMPLER Conditioned Stacker Accurate temperatureconditioned stacker that holds up to 24 shallow or 12 deep well plates. Integrated Organizer Holds LC-solvent bottles. Well Plate Waiter Automatically transports the required tray to the autosampler. Closed Environment Ensures optimal conditions for storage and processing. Autosampler 5th generation high throughput autosampler with minimal carry-over. High speed concentric dual needle Ensures fast and reliable sampling. Reliance Autosampler High Throughput conditioned autosampler offers maximum throughput and flexibility in sample introduction. The highest quality and reliability standards are met. SparkLink Software Instrument control software with integration for all major MS data packages.

9 RELIANCE TECHNICAL SPECIFICATIONS General specifications Sample Viscosity cp, user selectable for optimized cycle times. Sample Capacity 1 plate in autosampler, max. 24 shallow or 12 deep well plates in stacker. Plate types 48-vial plates, 96 shallow well, 96 deep well, 384 shallow well or user defined well plate. Vial Dimensions Max. height vials: 47 mm (incl. cap); min. height vials: 12.5 mm (incl. cap). Note: select plate type in properties (Sparklink ). Only rigid plate material. 6x8 vials, dimensions base plate 128 x 85.8 mm (optional). Loop Volume µl. Plate & Height Detection Missing plate & height detection by vial sensor. Wash Solvent 3 Solvents selectable on Syringe Dispenser Valve. Method programming Injection Methods Full loop injections Partial loopfill injections Zero sample loss injections. Injection Modes Speed mode Flex mode. Injection Volumes Mode Lower limit (µl) Upper limit (µl) Full loop Loop volume Loop volume Partial loop 5 1/2 Loop volume (max. loopvolume: 200 µl) Zero sample loss µl with Bio-needle Injections per Vial Max. 9 (volumes are programmable for each injection). Analysis Time Max. 9 hrs. 59 min. 59 sec. Needle Wash Volume Programmable µl, with 3 solvents. Analytical performance Options Capped and sealed vials/plates: Temperature Control Built-in Peltier cooling in autosampler and stacker. Full Loop Injections Reproducibility: RSD 0.3 %. Closed conditioned system for guaranteed constant Partial Loopfill Injections Reproducibility: RSD 0.5 %, injection volumes > 5 µl, temperature and humidity suitable for 24-hour with headspace pressure on the vial and 30 µl operation. Temperature is software programmable pre-flush with air segment. (Sparklink ) and computer-monitored. Zero sample loss Injections Integrated Column Switching (ISS) Reproducibility: RSD 1.0 %, injection volumes > 5 µl, Integrated high pressure stream switching valve. with headspace pressure on the vial. Biocompatible Option Rheodyne or Valco biocompatible valves, with PEEK Open vials/plates, valid at 1.0 cp: sample needle and PEEK flowpath. Full Loop Injections Reproducibility: RSD 0.3 %. Biocompatible Column Switching Valve ISS Partial Loopfill Injections Reproducibility: RSD 0.5 %; injection volumes > 5 µl, Mistral column oven combined with the Reliance. without headspace pressure on the vial and 30 µl The injection valve and optional column switching pre-flush with air segment. valve fit into the column oven. Zero sample loss Injections Bio-needle Specially designed sample needle for easier sampling Reproducibility: RSD 1.0 %; injection volumes > 5 µl. of serum, plasma and urine samples. without headspace pressure on the vial. Carry-over < 0.01 % with programmable needle wash. Specifications are subject to change without notice

10 P. O. BOX 388, 7800 AJ EMMEN, THE NETHERLANDS PHONE (31) , FAX (31) WEBSITE System System Components Solutions TECHNOLOGY LEADER IN AUTOMATED FRONT END SAMPLE HANDLING Copyright 2003 Spark Holland BV All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form by any means, without written permission from Spark Holland BV. Spark Holland BV reserves the right to make changes to the design and specifications of the Reliance without prior notice. Reliance and Symbiosis are trademarks of Spark Holland BV.