ChromaNik Technologies Inc.

Transcription

1 HPLC column Chromaik Technologies Inc.

2 The evolution of further surface modification * Development of a novel silyl-reagent which bonded with multifunctional endcapping reagents Final TMS treatment C8 phase can be used at ph range from to as well as hybrid C8s. An excellent peak for acidic, basic and metal chelating compounds without effect of residual silanol groups Characteristics of SunArnor Column name Particle size (µm) Pore diameter (nm) Specific surface area (m /g) Carbon loading (%) Phase ph range for usage USP Category C8 and C8 - L RP-AQUA and C8-0 Equivalent to L6 C8 / RP-AQUA

3 Stability under basic ph condition C8 Almost same stability to compare with the hybrid C8s. Relative plate / % C8 Company A hybrid C8 Company B Hybrid C Elution time / h Durable test condition Column size: 50 x. mm Mobile phase: H/0mM Ammonium bicarbonate (ph0.5)=0/70 Flow rate: 0.8 ml/min Temperature: 60 ºC Measurement condition Column size: 50 x. mm Mobile phase: C/H =60/0 Flow rate: 0. ml/min Sample: = Butylbenzene Stability under neutral ph condition at 80 o C C8 Relative plate / % C8 Company C C8 AQ Company D C Elution time / h Durable test condition Column size: 50 x. mm Mobile phase: H/0mM Ammonium acetate (ph6.8)=0/70 Flow rate: 0. ml/min Temperature: 80 ºC Measurement condition Column size: 50 x. mm Mobile phase: C/H =60/0 Flow rate: 0. ml/min Sample: = Butylbenzene The result of the comparison data in this catalog is not the representative example of all application. C8 / RP-AQUA

4 Stability under basic ph condition RP-AQUA Stability under 00% aqueous buffer (ph0) 00 Relative plate / % RP-AQUA Elution time / h Durable test condition Column size: 50 x. mm Mobile phase: 0mM Ammonium bicarbonate (ph0) Flow rate: 0. ml/min Measurement condition Column size: 50 x. mm Mobile phase: C/H =70/0 Flow rate: 0. ml/min Sample: = Butylbenzene C8 / RP-AQUA

5 Parameter using standard sample Evaluation of hydrogen bonding, hydrophobicity and steric selectivity Reproducibility in retention Separation of standard sample of C8 and RP-AQUA Condition Column: C8 and RP-AQUA 5 μm, 50 x.6 mm Mobile phase: H/H =75/5 Flow rate:.0 ml/min Sample: H = Uracil = Caffeine = Phenol H H H C Retention time / min = Butylbenzene 5 = o-terphenyl 6 = Amylbenzene 7 = Triphenylene Hydrogen bonding (Caffeine/Phenol) Hydrophobicity (Amylbenzene/Butylbenzene) Steric selectivity (Triphenylene/o-Terphenyl) C RP-AQUA Reproducibility in retention of C8 Batch 0 Batch 0 Batch 0 Batch 0 Batch Retention time/min Retention time of amitriptyline Batch Condition (amitriptyline) Column dimension: 50 x.6 mm Mobile phase: Acetonitrile/0mM phosphate buffer ph7.0=(60:0) Flow rate:.0 ml/min Temp.: 0 o C Sample: =Uracil =Propranolol Retention time min min 0 8. min min min Average (Av) Standard deviation (σ) 7.9 min 0. min H H The result of the comparison data in this catalog is not the representative example of all application. = ortriptyline =Amitriptyline H C8 / RP-AQUA

6 Peak Shape of basic compound Comparison of amitriptyline () using ammonium acetate buffer Comparison of amitriptyline () using phosphate buffer C8, 5 μm tr=8. min TF=.0 C8, 5 μm tr=7.69 min TF=.08 Sunniest C8, 5 μm tr=8.8 min TF=.7 Sunniest C8, 5 μm Company A Hybrid C8, 5 μm tr=8. min TF=.0 tr=.95 min TF=.0 Company A Hybrid C8, 5 μm Company B Hybrid C8,.7 μm tr=6. min TF=.59 tr=5.7 min TF=.5 C8, 5 μm Company B Hybrid C8,.7 μm <tr: retention time, TF: tailing factor> Comparison of pyridine () using methanol/water mobile phase tr=5. min TF=. Condition (amitriptyline) Column dimension: 50 x.6 mm Mobile phase: A) Acetonitrile/0mM phosphate buffer ph7.0=(60:0) B) Acetonitrile/0mM ammonium acetate ph6.8=(0:60) Flow rate:.0 ml/min Temp.: 0 o C Sample: =Uracil =Propranolol = ortriptyline =Amitriptyline <tr: retention time, TF: tailing factor> H H H Sunniest C8, 5 μm Company A Hybrid C8, 5 μm Company B Hybrid C8,.7 μm Condition (pyridine) Column dimension: 50 x.6 mm Mobile phase: H/H =0/70 Flow rate:.0 ml/min Detection: UV@50nm Sample: = Uracil, = Pyridine, = Phenol H H H 5 C8 / RP-AQUA The result of the comparison data in this catalog is not the representative example of all application.

7 Peak shape of acidic and metal chelating compounds Comparison of oxine (metal chelating compound) tr=.55 min TF=.07 C8, 5 μm Comparison of formic acid (acidic compound) tr=.96 min TF=.9 C8, 5 μm tr=.77 min TF=.07 RP-AQUA, 5 μm tr=.0 min TF=.9 RP-AQUA, 5 μm tr=.57 min TF=. Company A Hybrid C8, 5 μm tr=.70 min TF=.8 Company A Hybrid C8, 5 μm tr=.9 min TF=.9 Company B Hybrid C8,.7 μm tr=.65 min TF=5. Condition (formic acid) Column dimension: 50 x.6 mm Mobile phase: C/0.% H P =/98 Flow rate:.0 ml/min Detection: UV@0nm Sample: = Formic acid = Acetic acid = Propionic Acid Company B Hybrid C8,.7 μm <tr: retention time, TF: tailing factor> Condition (oxine) Column dimension: 50 x.6 mm <tr: retention time, TF: tailing factor> Mobile phase: C/0mM H P =0/90 Flow rate:.0 ml/min Detection: UV@50nm Sample: = 8-Quinolinol (xine) H = Caffeine H C *Hybrid C8s showed a very poor peak shape for formic acid. It is doubted that some amines as a byproduct remained on the surface of packing materials. The result of the comparison data in this catalog is not the representative example of all application. C8 / RP-AQUA 6

8 Distributor network Technolab-Sorbent AB Biotech AB Inacom dichrom Instruments BioAnalytic Watrex Praha, s.r.o. Simkon Kft. Beijing Tai-Ke-Mei Hi-Tech Co., Ltd. brnuta faza j.d.o.o. Labquip LabService Analytica S.r.l. Moricon BGB Analytik AG BGB Analytik Yair Technologies Ltd Allytech Corporation Ltd. France S.A.S. ChromaTech Ltd Applied Chemical and Instrument Co., Ltd I-Tek Instruments Prochrome India Shahana BioMedicals Chromatopak Analytical IT Technologies Pte Ltd SiliCycle, Inc. Innovations United Eco-Chem Inc. S&B Analitica rdering information of Packings C8, μm C8, 5 μm RP-AQUA, μm RP-AQUA, 5 μm Inner diameter (mm) USP category Length (mm) Catalog o. Catalog o. Catalog o. Catalog o. Catalog o. 50 HB HB HB HB HB6 HB6 HB L 50 HB7 HB7 HB HB8 HB HB HB HB HB6 HB6 HB HB7 HB7 HB L 50 HB8 HB8 HB8 HB78 HB88 50 HR HR HR HR HR6 HR6 HR HR7 HR7 HR HR8 HR HR HR HR HR6 HR6 HR HR7 HR7 HR HR8 HR8 HR8 HR78 HR88 Equivalent to L6 Equivalent to L6 Guard Cartridge column of product Particle size Catalog o. C8, 5 μm Guard cartridge column (-pak + Holder) x 0mm 5 μm HBAH RP-AQUA, 5 μm Guard cartridge column (-pak + Holder) x 0mm 5 μm HRAH C8, 5 μm Guard cartridge (-pak ) x 0mm 5 μm HBAC RP-AQUA, 5 μm Guard cartridge (-pak ) x 0mm 5 μm HRAC Guard cartridge holder --- HHAC Manufacturer Chromaik Technologies Inc. 6-- amiyoke, Minato-ku, saka, Japan TEL: FAX: info@chromanik.co.jp URL: 60