Tissue Culture. Special Section. Tissue Culture. Consumables. Filtration. Roller Culture Apparatus Stirrers

Transcription

1 Special Section Tissue Culture Contents Page Consumables Serological pipettes Centrifuge tubes 1012 Roller bottles 1013 Cell culture flasks 1013 Erlenmeyer flasks 1013 Cell spreaders 1014 Dishes 1014 Multiple well plates 1014 Glass based dishes/plates 1015 Filtration Units Membranes 1020 Syringe filters 1020 Filling bells 1020 Roller Culture Apparatus Stirrers

2 Consumables Serological Pipettes A wide range of pipettes manufactured from crystal clear polystyrene. All pipettes up to 25ml are constructed in a single piece, eliminating the problem of sample hang-up and so providing greater accuracy (>98%). Each pipette has a colour coded plug for easy size identification. Graduations are fine and crisp, so easy to read with increased accuracy. The graduation also extends beyond the end of the scale to provide extra capacity when needed. Pipettes are available sterile, either individually wrapped in fibre-free paper/plastic sleeves or in plastic packs of 25. They are gamma irradiated, non cytotoxic, non haemolytic & non pyrogenic. The standard pipettes are also available non-sterile, in bulk zip-lock bags. Available to purchase in pack or case quantities. Standard narrow tip pipettes, suitable for general laboratory and tissue culture procedures Short pipettes, which are ideal for use in confined areas such as laminar flow cabinets Wide tip pipettes, designed for handling viscous solutions Open end pipettes, for the handling of very viscous solutions or suspensions 1.1 and 2.2ml milk and bacteriological pipettes Aspiration pipettes, non plugged and non graduated pipettes for transferring solutions where no accuracy is required and for vacuum aspirating procedures Capacity Plug Qty per Pack Cat. No. Price Case Cat. No. Price ml colour sterile bag Standard Narrow Tip Pipettes 1 Yellow POA POA 1 Yellow POA POA 2 Green POA POA 2 Green POA POA 5 Blue POA POA 5 Blue POA POA 10 Orange POA POA 10 Orange POA POA 25 Red POA POA 25 Red POA POA 50 Purple POA POA Short Pipettes 5 Blue POA POA 10 Orange POA POA Wide Tip Pipettes 5 Blue POA POA 5 Blue POA POA 10 Orange POA POA 10 Orange POA POA Open End Pipettes 1 Yellow POA POA 1 Yellow POA POA 2 Green POA POA 2 Green POA POA 5 Blue POA POA 5 Blue POA POA 10 Orange POA POA 10 Orange POA POA Milk & Bacteriological Pipettes 1.1 Yellow POA POA 1.1 Yellow POA POA 2.2 Green POA POA 2.2 Green POA POA Aspiration pipettes 2 No plug POA POA 10 No plug POA POA Non-Sterile Standard Pipettes 1 No plug POA POA 2 No plug POA POA 5 No plug POA POA 10 No plug POA POA to

3 Consumables to Corning, Serological Pipettes A range of pipettes suitable for sterile tissue culture applications. Available in 2 styles of individual wrapping; paper/plastic wrap and all plastic wrap. The paper/plastic wrap is colour coded and allows easy opening by either pop-through or peel-apart techniques. The all plastic banana-peel wrap provides easy, aseptic opening and full view of the pipette for easy size identification. Cat. No. Capacity, Graduations Negative Colour Coded Pack Case Price/ ml Grads. Stripe Quantity Case Serological pipettes individually paper/plastic wrapped Yellow POA Green POA Blue POA Orange POA Red POA Purple POA Serological pipettes individually all plastic wrapped Yellow POA Orange POA Red POA Corning, Plastic Centrifuge Tubes Corning centrifuge tubes feature black printed graduations and a large white marking spot. They are available in 15ml and 50ml sizes and a choice of material, either polypropylene or polyethylene teraphthalate. The 15ml tubes are conical and the 50ml tubes are available either conical bottom or self standing. These tubes are available with your choice of cap style, the original plug seal or alternatively the flat top rim seal. Both of these provide a tight, secure seal. All tubes are sterile and certified non-pyrogenic. They are available in racks or bulk packs. Cat. No. Material Cap Style Max. RCF 1, Sterile Pack Case Price/ xg Type Quantity Case 15ml Centrifuge Tubes PET 2 Flat top 3600 Bulk packs of POA PET Flat top 3600 Racks of POA PP 3 Flat top 8400 Bulk packs of POA PP Flat top 8400 Racks of POA PET Plug seal 3600 Bulk packs of POA PET Plug seal 3600 Racks of POA PP Plug seal 8400 Bulk packs of POA PP Plug seal 8400 Racks of POA Conical 50ml Centrifuge Tubes PP 3 Flat top 9400 Bulk packs of POA PP Flat top 9400 Racks of POA PP Plug seal 9400 Bulk packs of POA PP Plug seal 9400 Racks of POA PET 2 Plug seal 3600 Racks of POA Self Standing 50ml Centrifuge Tubes PP 3 Flat top 3000 Bulk packs of POA PP Plug seal 3000 Bulk packs of POA 1. RCF = Relative Centrifugal Force (xg) 2. PET = Polyethylene Teraphthalate 3. PP = Polypropylene Corning, Centrifuge Tubes and Support Cushions These tubes are ideal for applications requiring large-volume centrifugation. Each case of tubes contains a recyclable polyfoam rack to facilitate handling. Polycarbonate support cushions must be used with this product unless the rotor has V-bottom holders. Tubes are sterile, certified non-pyrogenic. Max. Relative Size Cap Centrifugal Sterile Case Price/ Cat. No. Description ml Style Force Pack Size Quantity Case Centrifuge tube, polypropylene 250 Plug POA Support cushion, polycarbonate 250 n/a n/a n/a 6 POA Centrifuge tube, polypropylene 500 Plug POA Support cushion, polycarbonate 500 n/a n/a n/a 6 POA 1012

4 Consumables Corning, Quick Seal and Plug Seal Roller Bottles Corning roller bottles are manufactured from virgin polystyrene and are treated for optimal cell attachment. Bottles feature superior optical clarity and a seamless one-piece durable construction that reduces damage caused by transit and accidental dropping. All bottles are certified non-pyrogenic and have individually printed lot numbers to aid in product traceability. Quick Seal bottles have printed graduations, every 50ml up to 200ml. Surface Sterile Case Price/ Cat. No. Area, cm 2 Graduations Cap Style Pack Size Quantity Case No Plug Seal 2 40 POA Yes Quick Seal 5 20 POA Corning, Quick Seal Caps Corning Quick Seal caps are suitable for use with Quick Seal bottles. Cat. No. Description Case Price/ Quantity Case Quick Seal caps 100 POA Corning, Expanded Surface Roller Bottles The ribbed design provides twice the growth surface with the same exterior dimensions. Two window areas provide for microscopic viewing as well as pouring surfaces. Bottles are treated for optimal cell attachment, certified non-pyrogenic, and have individually printed lot numbers to aid in product traceability. Cat. No. Surface Graduations Cap Style Sterile Case Price/ Area, cm 2 Pack Size Quantity case Yes Easy Grip 2 36 POA Corning Cell Culture Flasks Corning flasks feature an anti-tip skirt for increased stability, printed lot number for ease in traceability, and are certified non-pyrogenic. Flasks are available with a choice of cap style to match your application. Cat. No. Growth Flask Cap Sterile Case Price/ Area, cm 2 Description Style Pack Size Quantity Case Canted Plug POA Canted Phenolic POA Canted Vented POA Canted Plug POA Canted Phenolic POA Canted Vented POA Straight Plug POA Canted Plug 5 50 POA Canted Phenolic 5 50 POA Canted Vented 5 50 POA Angled Plug 5 50 POA Angled Vented 5 50 POA Angled Plug 5 25 POA Corning, Sterile Erlenmeyer Flasks Corning sterile, disposable, plastic Erlenmeyer flasks are made from optically-clear polycarbonate with a wide, easy-access mouth. They are designed as culture flasks or storage vessels and are ideal for shaker culture applications. The polypropylene plug seal cap offers two positions, open for gas exchange or closed for liquid-tight seal. The vented cap is supplied for use in open systems. Cat. No. Capacity Graduations Neck, mm Cap Sterile Case Price/ ml ml Styles Pack Size Quantity Case Plug 1 50 POA Plug 1 50 POA Plug 1 25 POA Vented 1 50 POA Vented 1 50 POA Vented 1 25 POA to

5 Consumables Cell Spreaders, Stainless Steel Stainless steel cell spreaders with smooth triangular, upturned ends that will not cut into agar. They are flame and autoclave sterilisable. The smaller 16mm bar cell spreader is ideal for 60mm plates and the larger 25mm bar version is for 90mm plates. Both spreaders have a handle length of 203mm. Cat. No. Description Bar width, mm Pack Price Small cell spreader 16 1 POA Large cell spreader 25 1 POA Disposable Cell Spreaders, Polypropylene Economical polypropylene L-shaped cell spreaders, ideal for microbiological applications. Their smooth surface enables quick spreading without tearing agar plates or disturbing media layers. They are supplied sterile in bags of 10 for immediate use without flaming. Cat. No. Description Pack Price Disposable cell spreaders 500 POA Corning, Cell Culture Dishes Manufactured from optically-clear virgin polystyrene. Available untreated or treated for optimal cellattachment. Treated dishes are sterilised by gamma radiation and certified non-pyrogenic. All dishes have stacking beads to aid in handling and are supplied with vents to provide consistent gas exchange. Cell Culture Dishes Non-treated Culture Dishes Size Growth Sterile Case Cat. No. Price/ Cat. No. Price/ (dia.xd), mm Area, cm 2 Pack Size Quantity Case Case 35x POA POA 60x POA POA 60x15 21 gridded POA 100x POA POA 100x * POA 150x POA POA 245x POA * Denotes 6-pack carrier Costar, Cell Culture Clusters Costar multiple well plates are treated for optimal cell attachment, sterilised by gamma radiation, and are certified non-pyrogenic. All plates have a universal footprint and a raised bead to aid in stacking. Alphanumeric markings provide well identification. Number of Well Growth Sterile Case Price/ Cat. No. Wells Dia, mm Area, cm 2 Pack Size Quantity case POA POA POA POA POA POA Costar, 96 Well Cell Culture Clusters Costar 96 well plates are treated for optimal cell attachment, sterilised by gamma radiation, and are certified non-pyrogenic. All plates have a universal footprint and a raised bead to aid in stacking. Alphanumeric markings provide well identification. Well Growth Sterile Case Price/ Cat. No. Configuration Dia, mm Area, cm 2 Pack Size Quantity Case Flat POA Flat POA Flat POA Flat POA Round n/a 1 50 POA Round 6.4 n/a 1 50 POA V-bottom POA Half Area POA Lid only n/a n/a POA 1. Provided with special low evaporation lids 3. 8-well strip, packaged 12 strips per plate 2. Provided without lid 4. Polypropylene with polystyrene lid 1014

6 Consumables Iwaki, Glass Based Dishes These sterile dishes combine a culture environment with the optical surface required for high definition microscopy. They comprise a glass or quartz coverslip attached to the base of a 35mm polystyrene dish with non-toxic silicone adhesive. Available with a choice of either 12 or 27mm base diameter, they are ideal for observing live or dead cells using a confocal or light microscope. With the benefit of excellent transmittance and low fluorescence, they allow sensitive fluorescence measurements to be made at high magnification. Being glass-bottomed they are also ideal for use with differential interference contrast (DIC) techniques in light microscopy. Supplied sterile, individually wrapped to Cat. No. Description Base Material Coverslip Diameter, mm Pack Price Glass based dish Glass POA Glass based dish Glass POA Glass based dish Quartz POA Glass based dish Quartz POA Iwaki, Glass Based Culture Plates Glass based culture plates are specifically designed for the fluorescent measurement of live and dead cells at a higher magnification than that achievable through plastic dishes. With a base thickness /- 0.02mm they have superior optical clarity over conventional polystyrene alternatives, making them ideal for high transmittance microscope scanning. The chimney well design of the black plates reduces cell to cell contamination and cross talk. They have a low fluorescent background and are especially suitable for use in sensitive fluorescence and confocal microscopy. Standard multiwell plate format Supplied sterile, individually packed with own lid Cat. No. Description No. of Wells Well Bottom Growth Area, cm 2 Pack Price Glass based culture plate 6 Flat POA Glass based culture plate 24 Flat POA Glass based culture plate 96 Flat POA Iwaki, Glass Based Assay Plates Glass based culture plates are specifically designed for the fluorescent measurement of live and dead cells at a higher magnification than that achievable through plastic dishes. With a base thickness /- 0.02mm they have superior optical clarity over conventional polystyrene alternatives, making them ideal for high transmittance microscope scanning. The low base design ensures readability of all wells by inverted microscopes. The chimney well design of the black plates reduces cell to cell contamination and cross talk. They have a low fluorescent background and are especially suitable for use in sensitive fluorescence and confocal microscopy. Imaging plane from bottom of plate less than or equal to 0.5mm Flatness across focal plane less than or equal to 100µm Standard multiwell plate format Supplied non sterile in inner packs of 5 Ideal for applications such as receptor ligand detection through fluorescent probes, cell based assay and low end sensitivity detections. Cat. No. Description No. of Well Well cap. Growth Pack Price Wells Bottom µl Area, cm Glass based assay plate 96 Flat POA Glass based assay plate 384 Flat POA

7 Filtration Nalgene, MF75 Series of Filter Units and Bottle Top Filters The MF75 series of filter units (except 115ml) are designed with an easy to use shape, with tapered sides and grip dimples for secure handling and a wide base for stability. The lot number, membrane type, pore size and expiration date are printed on filter units, bottle tops and receivers for easy identification and lot traceability. The housing is manufactured from polystyrene, with a choice of membrane types and pore sizes. The 115ml filter units have low profile polystyrene housing and a wide base, providing the ideal unit for small volume benchtop filtration. The units have colour coded filter collars for instant identification of the membrane type. Every lot is certified sterile, non-pyrogenic and non-cytotoxic. Sterile shelf life is 5 years Nalgene, MF75 Series CN Membrane Filter Units Cellulose nitrate (CN) is the standard membrane for general filtering of buffers and solutions. Colour coded green. The filter units have graduations on both uppers and receivers and the sidearm has a cellulosic vent plug and quick-disconnect tubing adaptor. Supplied with a leak-proof, threaded closure for the receiver. Cat. No. Upper Receiver Pore Membrane Membrane Grid Pack Price Capacity Capacity Size Diameter Colour ml ml µm mm White None 12 POA White Green 12 POA White None 12 POA White Green 12 POA White Black 12 POA White None 12 POA White Green 12 POA White Black 12 POA White None 12 POA White Green 12 POA White Black 12 POA White None 12 POA White Green 12 POA White Black 12 POA White None 12 POA White Green 12 POA White Black 12 POA 1. Low profile units 2 Normal profile sterilisation filter units 3. Each pack includes 12 glass-fibre prefilters Nalgene, MF75 Series Nylon Membrane Filter Units Nylon membranes are used for special applications. They have no wetting agents, the lowest levels of extractables and provide excellent alcohol resistance. Colour coded red. The filter units have graduations on both uppers and receivers and the sidearm has a cellulosic vent plug and quick-disconnect tubing adaptor. Supplied with a leak-proof, threaded closure for the receiver. Cat. No. Upper Receiver Pore Membrane Pack Price Capacity Capacity Size Diameter ml ml µm mm POA POA POA POA POA POA POA POA POA POA 3. Each case includes 12 glass-fibre prefilters 4. Media-Plus filter units 1016

8 Filtration Nalgene, MF75 Series SFCA Membrane Filter Units and Bottle Top Filters Surfactant-free cellulose acetate (SFCA) is much cleaner than standard cellulose acetate. It is low protein binding with no wetting agents and excellent flow rates. It minimises clogging and prevents loss of specific proteins. Colour coded yellow. The filter units have graduations on both uppers and receivers and the sidearm has a cellulosic vent plug and quick-disconnect tubing adaptor. Supplied with a leak-proof, threaded closure for the receiver and are designed for the filtration of large volumes (over 200ml) of whole serum. The large membrane surface provides excellent flow rates when filtering up to 500ml of whole fetal bovine serum or a lesser volume of other sera and media-plus filter units allow easy vacuum filtration of up to 1L of media with 20% serum. The graduated bottle top filters are designed for direct filtration into sterile glass media bottles, screwing securely onto media bottles with 33 or 45mm necks. Only sterile bottles designed for use with vacuum should be used. They have side-arms with quick-disconnect tubing adaptors. SFCA Membrane Filter Units Cat. No. Upper Receiver Pore Membrane Pack Price Capacity, ml Capacity, ml Size, µm Diameter, mm POA POA POA POA POA POA POA POA POA POA POA POA POA POA 1. Each pack includes 12 glass-fibre prefilters SFCA Membrane Bottle Top Filters Cat. No. Filter Fits Bottle Pore Membrane Pack Price Capacity, ml Neck Size, mm Size, µm Diameter, mm POA POA POA POA POA POA POA POA POA POA

9 Filtration Nalgene, MF75 Series PES Membrane Filter Units and Bottle Top Filters Polyethersulfone (PES) is the ultimate cell and tissue culture media membrane, with low protein binding, low extractables, no external wetting agents and a fast flow. These are ideal for biological and pharmaceutical sterilisation requirements. The 0.2µm filters are fitted with the new Supor machv membranes. These are super clean membranes that provide even faster throughput and very low protein binding. Colour coded blue. The filter units have graduations on both uppers and receivers and the sidearm has a cellulosic vent plug and quick-disconnect tubing adaptor. Supplied with a leak-proof, threaded closure for the receiver. The graduated bottle top filters are designed for direct filtration into sterile glass media bottles, screwing securely onto media bottles with 33 or 45mm necks. Only sterile bottles designed for use with vacuum should be used. They have side-arms with quick-disconnect tubing adaptors. PES Membrane Filter Units Cat. No. Upper Receiver Pore Membrane Pack Price Capacity, ml Capacity, ml Size, µm Diameter, mm * POA POA * POA POA * POA POA * POA POA * POA POA * POA POA PES Membrane Bottle Top Filters Cat. No. Filter Fits Bottle Pore Membrane Pack Price Capacity, ml Neck Size, mm Size, µm Diameter, mm * POA POA * POA POA * POA POA * POA POA * POA * POA * Fitted with the new, faster, Supor machv membrane Nalgene, Filter Unit Receivers For use with the MF75 series of filter units with an easy to use design. They are radiation sterilised and can be used to store sterilised filtrate. They are also handy as presterilised, single use storage containers. 1-1/2 turn threaded screw closure guarantees a leakproof seal and eliminates ph shift in receivers. Graduated. Cat. No. Description Capacity, ml Pack Price Filter unit receiver POA Filter unit receiver POA Filter unit receiver POA Filter unit receiver POA 1018

10 Filtration Nalgene, Analytical Filter Units Precise, easy to use systems for the recovery and growth of micro-organisms in research and industry. The disposable, preassembled, presterilised filter units have polypropylene uppers and polystyrene lowers. They have triton-free CN (cellulose nitrate) membranes, which provide superior recovery and growth of microorganisms. Uppers and lowers twist apart for easy removal of the membrane for culturing. The wide base is stable on the bench and the non-silicone gasket minimises the risk of contamination. They are excellent for microbial analysis, sterility testing, water quality or environmental testing. Cat. No. Upper Receiver Pore Membrane Membrane Grid Pack Price Capacity Capacity Size Diameter Colour ml ml µm mm White None 12 POA White Grey 12 POA Nalgene, Analytical Test Filter Funnels Disposable, sterile filter funnels for microbiological analysis testing, food/beverages, raw material, sterility testing and finished product quality. The polypropylene funnels with high-impact polystyrene collars are supplied preassembled and presterilised. The triton-free CN (cellulose nitrate) membranes provide superior recovery and growth of micro-organisms and are easily removed for analysis has a grey, water quality CN membrane with a white grid. The membrane turns black when wet, making it easier to detect and count growing colonies. Six funnel adaptors are included in each pack. Cat. No. Capacity Pore Membrane Membrane Grid Pack Price ml Size, µm Diameter, mm Colour White None 50 POA White Grey 50 POA White None 50 POA White Grey 50 POA Grey White 50 POA to Nalgene, Reusable Filterware The unique Nalgene filter holder family is a versatile system with interchangeable components in three useful volumes and various configurations. For use with 47/50mm membranes. Permits sterilisation or analytical filtration, using vacuum or pressure. Major holder components are moulded of durable, highly break-resistant, transparent polysulfone (PSF), which is non-toxic, exhibits low protein binding characteristics, contains very low levels of trace metals or organic leachables and has fairly good chemical resistance. Filter Holder with Receiver Eliminates the need for a manifold and/or breakable glass vacuum flask, so is suitable for field work. Supplied with a sterilisation membrane support plate for optimal flow rate and throughput and an analytical plate for optimal membrane support and to keep the membrane flat. The receiver is graduated. The two side-arms allow connection to a vacuum line; also accepts syringe filters. The tubing adaptor can be plugged with cotton for sterile venting. Accepts 6 to 8mm ID vacuum tubing. A cover allows convenient storage of sterile filtrate. Autoclavable. Cat. No. Chamber Receiver Nominal Filter Nominal Filter Pack Price Capacity, ml Capacity, ml Area 1*, cm 2 Area 2**, cm POA POA POA * Filter area 1 with analytical support plate. ** Filter area 2 with sterilisation support plate Bottle Top Filters This polysulfone filter holder with silicone O-ring and polypropylene vacuum adapter offers the convenience of a bottle top filter with the cost savings of a reusable filter housing. Fits glass media bottles with 33 or 45mm necks. The removable sterilisation membrane support plate provides maximum flow rate and throughput. There is a vacuum port on the lower section. The tubing adaptor can be plugged with cotton for sterile venting. Accepts 6 to 8mm ID vacuum tubing. Non-cytotoxic and autoclavable. Cat. No. Chamber Capacity, ml Neck Diameter, mm Nominal Filter Area, cm 2 Pack Price POA POA POA POA

11 Filtration Nalgene, Water Quality Membrane This cellulose nitrate (CN) membrane is certified to meet the requirements of standard methods for water quality work. It contains no extractables that will inhibit or stimulate the growth of bacteria and has no significant effect on the ph of the medium. The sterilisation technique causes no toxicity, chemical or physical changes. The membranes are individually wrapped, sterile and autoclavable. Suitable for use with Nalgene reusable filterware (see page 1019). Specification Min. bubble point, psig 40 Min. flow rate, water, ml/min/cm 2 measured at 14.7psig 51 Max. temp, C 130 Cat. No. Membrane Diameter, mm Pore Size, µm Membrane Colour Grid Pack Price White Green 100 POA Nalgene, Syringe Filters SFCA Membrane Syringe Filters Suitable for cold sterilisation of aqueous solutions, biological or immunological samples and cell culture media components. Surfactant free cellulose acetate (SFCA) is low protein binding with low extractables and contains no wetting agents. The housing material is modified acrylic. The inlet is female Luer- Lok and the outlet male Luer-Lok. The sterile version is certified sterile, non-cytotoxic and non-pyrogenic and is supplied in individual blister packs. The non sterile version is bulk packed. Do not autoclave. PES Membrane Syringe Filter The universal tissue culture membrane. This offers higher flow rates, lower protein binding and lower extractables than competitive cellulosic membranes. Inherently hydrophilic; contains no wetting agents. Suitable for the sterilisation of microbial media, proteinaceous solutions and tissue culture reagents. The housing material is modified acrylic. The inlet is female Luer-Lok and the outlet male Luer slip. Certified sterile, non-cytotoxic and non-pyrogenic and supplied in individual blister packs. Do not autoclave. Nylon Membrane Syringe Filters For universal filtration of HPLC and GC solvents and DMSO. Chemically resistant to a wide range of solvents, low levels of extractables and inherently hydrophilic, containing no wetting agents or plasticisers. Polypropylene housing material. Female Luer-Lok inlet and male Luer slip outlet. Bulk packed. Autoclavable. Specifications Membrane SFCA SFCA SFCA SFCA PES Nylon Nylon Sterile Yes Yes No No Yes No No Volume, ml Pore size, µm Membrane diameter, mm Filter area, cm Housing burst pressure, psig/bar 75/5.1 75/5.1 75/5.1 75/5.1 75/5.1 90/6.2 75/5.1 Bubble point, psig/bar 45/3.1 28/1.9 45/3.1 28/1.9 35/2.4 42/2.8 25/1.7 Hold up vol. after air purge, ml Nominal flow rate, ml/min 90 a 180 a 90 a 180 a 22 b 35 c 95 c Pack Cat. No Price POA POA POA POA POA POA POA a. At 14.7 psig/1 bar with water b. At 45 psig/3.1 bar with water c. Water at 13.5 psig Nalgene, Filling Bell, Polycarbonate A transparent, autoclavable filling bell for use in sterile work areas. Ideal for transferring sterile media aseptically from large containers to media bottles or culture vessels. Polycarbonate is durable, non-toxic and will not shatter, making an economical alternative to glass. It fits all common media bottles and is supplied with an autoclavable polypropylene tubing adaptor, which accepts 6.3, 9.5 and 12.7mm ID tubing. Specification Overall height with adaptor, mm 114 Bell ID, mm 70 Inner fill tube length, mm 63 Inner fill tube bottom OD, mm 9 Inner fill tube bottom ID, mm 5 Cat. No. Description Pack Price Filling bell 1 POA 1020

12 Roller Culture Wheaton, Roller Culture Apparatus Conventional roller culture equipment designed to roll vessels 108 to 121mm in diameter and up to 290mm long. Two outside rollers can be moved inward to accommodate bottles as small as 75mm in diameter. 2 vessels per deck. An extremely compact unit, well-suited for research work. Supplied without vessels. Use in an environment of CO 2 may necessitate more frequent replacement of motor brushes. Maximum temperature for use is 40 C. CE marked. Specification Number of decks 1 Electrical requirements V AC, 50/60Hz, 14watts Bottle speed 0.1 to 3.8rpm based on 110mm bottle Dimensions, mm 320x325x180 Weight, kg 8 Cat. No. Description Price Bench top roller culture apparatus for 2 bottles POA Wheaton, Deck Kit Attaches quickly and easily to above roller culture apparatus. Holds an additional 2 roller bottles per kit. The roller culture base unit ( ) will accept two additional decks. Kit consists of assembled roller deck, four support posts, drive belt, necessary hardware and assembly instructions. Weight 2.3kg. Cat. No. Description Price Deck kit POA Wheaton, Mini Bottle Roller Culture Apparatus The mini bottle roller culture apparatus is designed for small-scale mixing and agitation using bottles that are too small to be accommodated by the standard roller apparatus. It is perfect for 30ml or larger serum bottles, ml media bottles, or 38 x 200ml culture tubes. The compact size makes it suitable for laboratories with limited space and for use with standard incubators and cold rooms. Continuous rotation of cylindrical bottles at predetermined optimum speeds provides better distribution of media and uniform gassing. The mini bottle roller culture apparatus will accommodate bottles 38 to 60mm in diameter, up to 240mm long, with bottle speeds of 3 to 45rpm (38mm bottle) and 2 to 30rpm (60mm bottle). It is a single deck unit which has four rolling positions, each position will accomodate bottle/s up to 240mm long. Up to two mini bottle deck kits can be added. Supplied without vessels. Use in an environment of CO 2 may necessitate more frequent replacement of motor brushes. Maximum temperature for use is 40 C. CE marked. Specification Number of decks 1 Electrical requirements 230V AC, 50/60Hz, 14watts Bottle speed 38mm dia. bottle - 3 to 45rpm 60mm dia. bottle - 2 to 30rpm Dimensions, mm 320x325x180 Weight, kg 8 Cat. No. Description Price Bench top roller culture apparatus for mini bottles POA Wheaton, Deck Kit Attaches quickly and easily to above roller culture apparatus. Holds an additional 4 roller bottles per kit. The roller culture base unit ( ) will accept two additional decks. Kit consists of assembled roller deck, four support posts, drive belt, necessary hardware and assembly instructions. Weight 4.1kg. Cat. No. Description Price Deck kit POA shown with two deck kits ( ) 1021

13 Roller Culture with 8 additional decks Wheaton, Modular Cell Production Apparatus The Wheaton modular cell production apparatus allows large scale propagation of monolayer cell cultures in standard roller culture vessels, 108 to 121mm in diameter and up to 550mm long. A powerful motor drives the bottles through a series of durable non-slip belts to exacting speeds from to 6.9 rpm (110mm bottles) with an accuracy of ±1%. The base unit consists of the control housing, controls and motor. Additional 5-position decks can be mounted on the base to accommodate increased numbers of roller vessels. As a guideline, a base unit with 9 decks (max) will hold enough glass or plastic bottles to give a total surface area of up to cm 2 for cell growth. The front wheels are equipped with locking devices to ensure stability. The unit may be ordered completely assembled or with base unit and decks separate for customer assembly. Supplied without bottles. Dimensions of unit with roller deck is 760x620x350mm. Each additional deck increases height of unit by 180mm. Electrics: 220/240V AC, 60 Hz, 35W. The all-position drive model is a positive traction drive system for lightweight plastic bottles, where each roller is individually driven. The all-position drive option may be field retrofitted (please enquire) or factory installed (see below). A locking speed control knob which prevents accidental change in rotation rates is included. Cat. No. Description Price Base unit with one 5-position deck 1 POA Additional deck (maximum 8) POA Base unit with one 5-position deck and all-position drive 2 POA Additional deck with all position drive (maximum 8) POA 1. Modular roller apparatus holds a maximum number of 9 decks. Not suitable for use with plastic bottles. 2. Modular roller apparatus holds a maximum number of 9 decks. Suitable for glass and plastic bottles. Optional rotation alarm and battery backup systems are available, please contact our Technical Office on for details. Wheaton, R 2 P Roller Culture Apparatus This versatile design allows for growth and requirement changes without having to revalidate new equipment. Modular design Programmable soft start and stop From 5 to 55 positions per unit (holds 10 to 10 program storage registers 110, 850cm 2 bottles) All-position drive system Displays accurate RPM by entering vessel Optional temperature monitoring, 1 to 4 diameter sensors per unit Wide speed range, brushless, DC motor, Optional battery back-up, complete 0.25 to 8.75rpm functionality for up to 18 hours Visual, audible, built-in rotation alarm Accepts glass or plastic bottles 108 to Programmable rotation direction, fixed or 121mm in diameter and up to 500mm long reversing Specification Bottle speed 0.25 to 8.75rpm (based on 110mm diameter bottles)* Accuracy, rpm ±0.01 Electrical requirements VAC, 50/60Hz, 35W Weight, kg Drive unit with one deck is 24kg, each additional deck is 7 11kg Dimensions (wxdxh), mm Drive unit with one deck 787x622x343 (for each additional deck add 152mm) * Other speed ranges are available, please enquire for details shown inside CO 2 Roll-in Incubator Cat. No. Description Price R 2 P roller culture apparatus with 1 deck POA R 2 P roller culture apparatus with 3 decks POA R 2 P roller culture apparatus with 11 decks POA R 2 P additional deck kit (factory fitted)* POA R 2 P additional deck kit (retro fittable) POA Accessories R 2 P temperature monitoring option (factory fitted)* POA R 2 P temperature monitoring option (retro fittable) POA R 2 P battery back-up unit (factory fitted)* POA R 2 P battery back-up unit (retro fittable) POA * Please note that this option must be specified at time of ordering. Optional computer software is also available, please enquire on for details. 1022

14 Roller Culture Wheaton, Roll-In Incubators for Cell Production Roller Apparatus Roll-In Incubator The Wheaton Roll-In incubator is a convenient, space-saving and economical alternative to a warm room for small production runs involving only one roller apparatus. Designed to accommodate a full size Modular or R 2 P Roller Apparatus with a base unit and nine roller decks. The unit provides over 38 cubic feet of space. Forced air circulation with digital temperature control provides temperature uniformity to ±0.5 C (without shelves). Temperature range is ambient +5 C to 70 C (empty chamber) Independent over-temperature safety protection Viewing window in door Four internal electrical outlets for accessories The incubator can accommodate up to six shelves for other types of equipment such as magnetic stirrers and rocker tables which can be plugged inside the unit. The Wheaton Roll-In Incubator has double-walled construction designed for temperature stability and easy cleaning. Specifications Heating method Forced air circulation with digital temperature control Temperature range, C Ambient +5 to 70* Temperature uniformity, C ±0.5* Interior dimensions, mm 889x660x1930 Exterior dimensions, mm 1041x851x2209 Electrics 230V, 60Hz, 1500W Weight, kg 331 Cat. No. Description Pack Price Roll-In Incubator with window 1 POA (shelves supplied separately) Shelf for roll-in incubator 1 POA For roller culture apparatus, please see page * In empty chamber Wheaton, Roll-In CO 2 Incubator The Wheaton CO 2 incubator is a convenient, space-saving and economical alternative to a warm room for small production runs. Designed to hold full size units, it can be used in combination with the optional shelves for spinner flasks, rocker tables, etc. The double walled construction provides for temperature stability and easy cleaning Forced air circulation with digital temperature control provides temperature uniformity to ±0.5 C (without shelves). Temperature range is ambient +5 C to 70 C (empty chamber) CO 2 range 0 20% Over 38 cubic feet of space Fold down ramps for rolling in Roller Culture Apparatus Independent over-temperature safety protection Viewing window in door Four internal electrical outlets for accessories Specifications Heating method Forced air circulation with digital temperature control Temperature range, C Ambient +5 to 70* Temperature uniformity, C ±0.5* CO 2 range 0 to 20% Interior dimensions, mm 889x660x1930 Exterior dimensions, mm 1041x851x2209 Electrics 230V, 60Hz, 1500W Weight, kg 315 Cat. No. Description Pack Price CO2 Roll-In Incubator with window 1 POA (shelves supplied separately) Shelf for CO 2 incubator 1 POA For roller culture apparatus, please see page * In empty chamber shown with R 2 P roller culture apparatus ( ) and optional shelves shown with R 2 P roller culture apparatus ( ) 1023

15 Stirrers shown with culture vessels Techne, MCS Series of Biological Stirrers A range of five stirrers Seven culture vessels ranging from 125ml to 5000ml Calibrated speed control and interval stirring Unique stirring action The Techne range of biological stirrers is suitable for suspension cell culture and for microcarriers. Uniform suspension of cell cultures and microcarrier beads is achieved by a gentle stirring action which aids attachment and promotes circulation of the medium around the cells to encourage a high rate of growth. Continuous stirring is adjustable up to 80rpm; the stirrers also incorporate fully variable interval stirring, whereby on times between 6 seconds and 5 minutes, and off times between 2 minutes and 2 hours can be selected. Special SOFTSTART design ensures controlled acceleration of the stirrer rod to the selected speed, thus avoiding excessive turbulence within the medium on start-up and eliminating the risk of cell damage. Each MCS biological stirrer comprises a polished stainless steel platform which houses the motor and controls; all models are suitable for incubator and/ or warm room use. Specifications Model MCS-101L MCS-102L MCS-104S MCS-104L MCS-104XL Vessels per system Maximum vessels size, ml 5000 (3000 min) (only) Width, mm Depth (including mains plug), mm Height (excluding vessel location pips), mm Net weight (excluding vessels), kg Cat. No Price POA POA POA POA POA General Characteristics: All Models Speed range, rpm 0 to 80 Speed setting accuracy, rpm Better than 3 SOFTSTART speed control 20 seconds acceleration/20 seconds deceleration Interval stirring variable on-time 6 seconds to 5 minutes variable off-time 2 minutes to 2 hours Heat input to culture vessel Negligible. Continuous running produces no measurable increase in temperature Electrical supply all models 240/110V 50/60Hz Range of Stirrers and Vessels Specifications Techne, Glass Culture Vessels with Stirrer Rods Techne biological stirrers use special borosilicate glass culture vessels and stirrer rods, siliconised to reduce the possibility of cells attaching to and growing on the surfaces. Nominal working volume 125ml 250ml 500ml 1L 3L 5L 5L Gross volume, ml Working volume range, ml Height, mm Diameter, mm Top cap thread size, mm Side cap thread size, mm Port size, mm Number of side necks Cat. No Price POA POA POA POA POA POA POA 1024

16 Stirrers STEM Corporation, Ultra Slow Speed Stirrers A range of precision ultra-slow speed stirrers specially designed for biotechnology applications including cell culture stirring. Magnetic drivers with electronic control give a precise stirring action which is reproducible to better than one rpm, with minimal heat production. The splash proof, non-corrosive polymer case and 12V DC platform voltage make these units ideal for operation within fume cupboards, heated cabinets or incubators. Type A, pendulum, stirrers do not contact the bottom or sides of the vessel, preventing any grinding action. Large stirrer bars (eg. cat. no ) can be used with type B systems. Type A stirrer platforms have a point source magnet for use with STEM pendulum type stirrers (use of other pendulum-type stirrers should be verified). Type B stirrer platform have a bar magnet for suspended-bar or bar-type stirrer. AS635 and AS640 will accommodate both types due to their size Specifications Stir bar configuration Type A & B Type A Type B Model AS635 AS640 AS649 AS650 AS651 AS641 AS642 AS637 No. of positions 1x2L 4x2L 1x5L 1x10L 1x24L 1x5L 1x10L 1x24L Speed range, rpm Total stir capacity, L Maximum vessel diameter, mm Dimensions (lxw), mm 150x x x x x x x x260 Cat. No Price POA POA POA POA POA POA POA POA Cell Culture Bottles (autoclavable polycarbonate) with Pendulum (Type A) Stirrer Cat. No. Description Capacity, L Price Cell culture bottle with pendulum (Type A) 2.2 POA Cell culture bottle with pendulum (Type A) 12.5 POA Cell culture bottle with pendulum (Type A) 24 POA Stirrer Bars PTFE coated stirrer bars for use with Type B stirrer platforms. Cat. No. Description Size, mm Pack Price PTFE coated stirrer bar 60x10 5 POA PTFE coated stirrer bar 40x8 5 POA Nalgene, 2605 Magnetic Culture Vessel Polycarbonate; polypropylene closures; TEFLON TFE stirrer bars; polypropylene/tfe stirring assembly Specially designed for efficient top-to-bottom mixing at low speed and low shear. This lightweight breakresistant 1 litre culture vessel is excellent for use on a magnetic stir plate for small-volume scale-up applications. It is the only plastic vessel of its size specifically designed for use as a culture vessel. Two magnetic stirrer bars are included: a small version for bacteria and a large version for mammalian cells. The impeller height is adjustable. Graduations to 1 litre. Autoclavable. Specification Capacity, total/working, litres 2.2/1 Closure diameter, top/side, mm 63/38 Overall height x overall diameter, mm 266x137 Overall width, including ports, mm 190 Cat. No. Description Price Magnetic culture vessel with stirrer bars POA 1025