Synergy, Cytation and FLx800 Multi-Mode Microplate Readers

Transcription

1 Fluorescence Filter, Cube and Mirror List Synergy, Cytation and FLx800 Multi-Mode Microplate Readers Use this Guide to find part numbers and other details for filters, filter cubes and dichroic mirrors used in BioTek s Multi-Mode Microplate Readers. TABLE OF CONTENTS Accessory Selection Guide... 2 Standard Filters... 3 Standard Filters (continued)... 4 Custom Filters... 5 Dichroic Mirrors Fluorescence intensity... 6 Synergy LX pre-configured filter cubes.7 Synergy H1 and Cytation pre-configured filter cubes... 8 Synergy Neo2 pre-configured filter cubes... 9 Synergy Neo2 pre-configured filter cubes (continued) Appendix I - Drug Discovery Filter Sets BioTek Instruments, Inc. P.O. Box 998, Highland Park, Winooski, Vermont USA COPYRIGHT 2018 Phone: Fax: Outside the USA: customercare@biotek.com

2 ACCESSORY SELECTION GUIDE Please refer to the chart below for compatible filter-based fluorescence accessories for your BioTek reader. FLx800 Filters (standard, pg. 3; custom, pg. 5) Dichroic Mirrors (pg. 8) Pre-configured Cubes Synergy LX (pg. 7) Synergy 2 Synergy HTX Synergy H1 (pg. 9) Synergy Neo2 (pg. 10) Cytation (pg. 9) Note: Synergy HTX filters are compatible with discontinued Synergy HT. Synergy 2 filters and mirrors are compatible with discontinued Synergy 4 and H4 Synergy Neo2 filters, mirrors and cubes are compatible with discontinued Synergy Neo, unless otherwise noted. 2 P age

3 STANDARD FILTERS Standard filters are available for the most common applications and can be installed upon request on the instrument before it ships from BioTek Wavelength Synergy LX, HTX, FLx800 Synergy 2 Synergy H1, Cytation Synergy Neo2 284/ TRF lamp Tryptophan excitation 310/ / Tyrosine, O- aminobenzoyl excitation 7-methoxycoumarin and Quanta Blu excitation 330/ HTRF excitation 340/ Fura-2 excitation 340/ / NADH excitation and tryptophan MUB, caspace-3, europium chelate excitation 380/ Fura-2 and EBFP excitation 400/ / Porphyrin excitation, O- aminobenzoyl and 7- methoxycoumarin 420/ CFP, Alexa Fluor 430 excitation 420/ / CFP excitation and Quanta-Blu Attophos excitation and caspace-3 440/ NADH 450/ CBQCA excitation, NanoBRET 460/ NanoOrange excitation and EBFP and MUB 485/ Fluorescein, EGFP excitation and CFP 485/ Propidium Iodide excitation 495/ LanthaScreen 1 500/ YFP excitation 508/ Fura-2 516/ EGFP 520/ / Alexa Fluor 532 excitation, LanthaScreen 2 VIC excitation and Fluorescein and EGFP 3 P age

4 STANDARD FILTERS (CONTINUED) Wavelength Synergy LX, HTX, FLx800 Synergy2 Synergy H1, Cytation Synergy Neo2 530/ Tamra excitation 540/ Alexa Fluor 546, CY3, and rhod2 excitation and EYFP 540/ Alamar Blu, Amplex red, RFP excitation 545/ Rhodamine B excitation 560/ Cell Titer Blue excitation 560/ VIC 560/ / Attophos and CBQCA 5-ROX excitation, AF532, HEX and VIC 570/ AlphaScreen 575/ / ROX excitation and 5-Tamra, preferred VIC NanoOrange and Attophos 590/ Alexa Fluor 594 and Texas Red excitation and Cell Titer Blue 590/ / Rhod-2, Alexa Fluor 546, and CY3 Alamar Blu, Amplex Red, RFP and porphyrin 615/ Primary AlphaLISA 620/ HTRF/LANCE 620/ / ROX and Alexa Fluor 594 and Alexa Fluor 633 excitation Rhodamine B, europium chelate, CY5 excitation 635/ Texas Red 645/ Alexa Fluor / HTRF/LANCE 645/ / Texas Red and Propidium iodide CY5, AlphaScreen excitation 4 P age

5 CUSTOM FILTERS Custom filters are available for the less common applications where alternative filters might be required. They ship separately from the instrument, and have a typical lead time of 6 weeks. Wavelength Synergy LX, HTX, FLx800 Synergy 2 Synergy H1, Cytation Synergy Neo2 276/ TRF lamp Tyrosine excitation 280/ TRF lamp General protein excitation 325/ / / MUB excitation 380/ / wtgfp excitation 405/ / / BRET2 470/ / / / Alternative fluorescein excitation 500/ Alternative YFP excitation 515/ BRET2 520/ / / / Alternative fluorescein and EGFP 540/ Cy3, Alexa fluor 546 excitation 540/ Alternative fluorescein and EGFP 550/ Alexa Fluor 555 excitation 556/ Alamar Blu excitation, Alexa Fluor / Alamar Blu excitation 570/ Alexa Fluor / Alexa Fluor 546, Alexa Fluor 568 excitation 580/ LP NanoBRET 5 P age

6 CUSTOM FILTERS (CONTINUED) Wavelength Synergy LX, HTX, FLx800 Synergy 2 Synergy H1, Cytation Synergy Neo2 585/ / / / / / / Cy5 excitation Texas Red excitation, Amplex red, Alamar Blu 660/ Cy5.5 excitation 665/ / Cy5.5 excitation 671/ Cy5.5 excitation 680/ / / Wavelength Cytation, Synergy H1, 2, HTX, LX, FLx800 Synergy Neo2 700/ Cy / Cy / Cy / / Red PMT 781/ / / / DICHROIC MIRRORS FLUORESCENCE INTENSITY Dichroic mirrors are available in two sizes: full-size and half-size. Each Synergy H1, Cytation and Synergy Neo2 filter cube can contain up to two EX and EM filter pairs and can fit two half-size mirrors or one full-size mirror. The mirror holder on the Synergy 2 can fit up to two half-size mirrors and one full size mirror (full-size mirrors are required for fluorescence polarization measurements). Synergy LX filter cube can contain one filter pair and one halfsize mirror. Half-Size Full-Size Cut-off (nm) Excitation Range Emission Range % All except FP Tryptophan HTRF, MMP, Quanta Blu 6 P age

7 DICHROIC MIRRORS FLUORESCENCE INTENSITY (CONTINUED) Z -Lyte Attophos, CFP, Fluo-3 MUB, Europium Chelate, Hoechst 33258, Omnia, LanthaScreen, Adapta Fluorescein, Picogreen, FAM Rhodamine 123, YFP CY3, HEX, Rhodamine 6G Alamar Blu, Amplex Red, TAMRA ROX, Texas Red AlphaScreen CY5 Important note about dichroic mirrors on Synergy 2: Please see table below for Synergy 2 (Synergy 4, and H4) installation guidelines and compatible measurement technologies. Refer to user s manual for detailed installation instructions. Install in mirror holder position # Fluorescence Intensity Time-Resolved Fluorescence Fluorescence Polarization Full-Size Mirror 3 Yes Yes Yes Half-Size Mirror 1 or 2 Yes Yes No (HTRF mirror) 1 Yes Yes No 7 P age

8 SYNERGY LX PRE-CONFIGURED FILTER CUBES These are pre-configured filter cubes for common applications. Customized filter cubes can be assembled upon request. Part Number Content LUM filter cube (one is included with F configurations) Red filter cube Green filter cube Blue filter cube SYNERGY H1 AND CYTATION PRE-CONFIGURED FILTER CUBES These are pre-configured filter cubes for common applications. Customized filter cubes can be assembled upon request. Part Number Content Blue filter set: EX 360/40 EM 460/40 DM 400 Green filter set: EX 485/20 EM 528/20 DM 510 Green filter set: EX 485/20 EM 528/20 DM 510 Red filter set: EX 530/25 EM 590/35 DM HTRF filter set: EX 330/80 EM 620/10, 665/8 DM LanthaScreen terbium filter set: EX 340/30 EM 495/10, 520/25 DM Lance filter set: EX 360/40 EM 620/10, 665/8 DM Filter cube Luminescence TRF cube: EX 360/40 EM 620/40 DM Green FP filter set: EX 485/20 EM 528/20 DM 510, polarizers Red FP filter set: EX 530/25 EM 590/35 DM 570, polarizers Blue FP filter set: EX 360/40 EM 460/40 DM 400, UV polarizers Empty filter cube FP visible (EX > 400nm) Empty filter cube FP UV (EX between 300 and 400nm) Empty Filter cube - non FP applications Filter cube Luminescence BRET 1 cube: EM 460/40, 540/25 nm BRET 2 cube: EM 410/80, 515/30 nm Filter cube: EX 380/20, 485/20 EM 440/30, 528/ Deep UV FP filter set: EX 284/10 EM 340/30 DM Far Red FP filter set: EX 620/40 EM 680/30 DM 660, polarizers Far Red filter set: EX 620/40 EM 680/30 DM Green/LUM filter set: Green filter set EX 485/20 EM 528/10, DM Filter Cube: Blue (360/460), Red (530/590) Filter Cube: MitoXpress Xtra Filter Cube: PH-Xtra Glycolysis 8 P age

9 SYNERGY NEO2 PRE-CONFIGURED FILTER CUBES These are pre-configured filter cubes for common applications. Customized filter cubes can be assembled upon request. Cube type: SPECIAL Cube # Paired with Description / Application Alpha (96-/384-well plates): EM 570/ or Alpha top cube for dual PMT system and up Dual PMT single filter cube. Required for top measurement when paired with cubes and higher. Not for use with single PMT configurations of Synergy Neo to All dual FP assays Alpha (1536-well plates): EM 570/ AlphaPlex lower cube. For Synergy Neo2 use only, not compatible with Synergy Neo. Cube type: EXCITATION for DUAL assays (TR-FRET, FRET and Lum.) Cube # Paired with Description/Application HTRF-Europium: EX 330/80 DM , HTRF Terbium, LanthaScreen Terbium: EX 340/30 DM CFP-YFP FRET: EX 420/50 DM , 46, FITC-TRITC, EGFP-Cy3, EGFP-YFP FRET: EX 485/20 DM Cy3-Cy5 FRET: EX 540/25 DM GeneBLAzer, Z-Lyte: EX 400/30 DM LANCE: EX 360/40 DM 400 Cube type: EMISSION for DUAL assays (TR-FRET, FRET and Lum.) Cube # Paired with Description /Application 41 11, HTRF, LANCE red: EM 620/10,665/8 DM LanthaScreen: EM 495/10, 520/25 DM CFP-YFP FRET: EM 485/20, 540/25 DM FITC-TRITC FRET: EM 528/20, 575/15 DM Cy3-Cy5 FRET: EM 590/35, 680/30 DM EGFP-Cy3 FRET: EM 516/20, 590/35 DM EGFP-YFP FRET: EM 516/20, 540/25 DM GeneBLAzer, Z-Lyte: EM 460/40, 528/20 DM HTRF green: EM 520/10, 620/10 DM * BRET 1 Dual PMT, 460/ * BRET 2 Dual PMT, 410/ Dual Alpha (AlphaPlex): EM 540/25, 615/16 DM * NanoBRET: EM 450/50, 610LP DM 550 *Any lower cube with open LUM position 9 P age

10 SYNERGY NEO2 PRE-CONFIGURED FILTER CUBES (CONTINUED) Cube type: DUAL FP cubes, paired with cube #4 Cube # Paired with Description/Application 4 61 to REQUIRED to use cubes 61 to Dual FP Green: EX 485/20 EM 528/20 DM Dual FP red1: EX 530/25 EM 590/35 DM Dual FP red2: EX 590/20 EM 635/32 DM Dual FP far red: EX 620/40 EM 680/30 DM Dual FP double: EX 485/20, 530/25 EM 530/25, 590/35 DM 510, Dual FP UV: EX 360/40 EM 460/40 DM Dual FP UV: EX 340/30 EM 530/25 DM 400 Cube type: SINGLE PMT or DUAL PMT single lower cube (requires cube #3 as upper cube) Cube # Paired with Description / Application and up REQUIRED for top measurements for top GFP and LUM: EX 485/20 EM 516/20 DM for top GeneBLAzer: EX 400/30 EM 460/40, 528/20 DM for top FITC-TRITC FRET: EX 485/20 EM 528/20, 575/15 DM for top EGFP-Cy3 FRET: EX 485/20 EM 516/20, 590/35 DM for top EGFP-YFP FRET: EX 485/20 EM 516/20, 540/25 DM for top Cy3-Cy5 FRET: EX 540/25 EM 590/35, 680/30 DM for top MUB/SF: EX 360/40, 485/20 EM 460/40, 528/20 DM 400, for top Green FP: EX 485/20 EM 530/25 DM for top BRET2: EM 410/80, 515/ for top Far red FP: EX 620/40 EM 680/30 DM for top Fluorescein (green, red): EX 485/20, 530/25 EM 528/20, 590/35 DM 510, for top Delfia Europium and LUM: EX 360/40 EM 620/40 DM for top Omnia and LUM: EX 360/40 EM 485/20 DM for top Luminescence standard / for top Red, Far Red: EX 530/25, 620/40 EM 590/35, 680/30 DM 570, for top Fura 2: EX 340/11, 380/20 EM 508/20 DM for top BRET 1: EM 460/40, 540/ for top Deep UV FP: EX 284/10 EM 340/30 DM for top HTRF Red: EX 330/80 EM 620/10, 665/8 DM for top FP: EX 530/20 EM 590/20 DM for top Lum 485, Lum 600: EM 485/20, 600/ for top NanoBRET: EM 450/50, 610LP for top CUBE 123 MITOXPRESS XTRA ASBY for top CUBE 124 PH-XTRA GLYCOLYSIS ASBY for top HTRF Tb/Green for top Empty customizable filter cube and barcode 10 P age

11 APPENDIX I - DRUG DISCOVERY FILTER SETS Cisbio Assays HTRF Assay Technology Excitation Emission 1 Emission 2 Dichroic Invitrogen Assays 330/ / / nm Assay Technology Excitation Emission 1 Emission 2 Dichroic LanthaScreen Terbium Adapta and LanthaScreen Europium Omnia Z -Lyte GeneBLAzer PolarScreen (green FP) PolarScreen (red FP) Predictor (red FP) 340/ / / / / / / / / / / / / / / / / / / / nm nm nm nm (Bottom) 510 nm nm nm Perkin Elmer Assays Assay Technology Excitation Emission 1 Emission 2 Dichroic LANCE DELFIA AlphaScreen 360/ / / / / / / nm nm nm Bellbrook Assays Assay Technology Excitation Emission 1 Emission 2 Dichroic Transcreener Far Red (FP) 620/ / nm P age