ZirChrom Separations

Transcription

1 ZirChrom Separations LC/MS friendly, ultra-durable zirconia-based (HPLC) stationary phases. Bene Technology Co., Ltd. MKT

2 HISTORY ZirChrom Separations, Inc. is a company formed in 1995 located in Anoka, Minnesota, USA. ZirChrom manufactures a full line of zirconia-based high performance chromatographic materials for the analytical analysis of compounds primarily by high performance liquid chromatography (HPLC). Silica gel, alumina or polymeric HPLC stationary phases, which are widely used today, are subject to poor performance under some operating conditions such as high mobile phase ph (> ph 8) and elevated column temperatures, respectively. Alternatively, zirconia is chemically stable over the entire ph range from 1-14, and is also thermally stable. Due to the toughness of zirconia, ZirChrom has adopted the rhinoceros( 코뿔소 ) as its company symbol. The extreme stability of zirconia and its unique chromatographic selectivity allows for the optimization of separation conditions, which are totally incompatible with other types of supports. As with the more traditional HPLC supports, a wide variety of analyses may be performed including amino acids, peptides, proteins, nucleic acids (RNA and DNA), carbohydrates, pesticides, environmental samples, clinical samples and pharmaceuticals to name just a few. Zirconia-based supports promise to revolutionize the way that liquid chromatographic analyses are done. These supports will last longer, and allow for faster more selective separations resulting in significant cost savings per analysis.

3 Effect of Mobile ph to Zr/Si 1. The ph of the mobile phase affects both zirconia s surfacecharge and the charge state of the analyte. 2. The type of buffer used in the eluent. 3. The pka of the analyte.

4 Zirconium vs Titanium vs Silicon Electron Configuration - Zirconium : [Kr] 5s 2 4d 2, (2,8,18,10,2 : 40 ; Zr 4+ ionic radius Å ) - Titanium : [Ar] 3d 2 4s 2, (2,8,10,2 : 22 ; Ti 4+ ionic radius Å) => strong electron donor-acceptor complexes of d orbitals - Silicon : [Ne] 3s 2 3p 2, (2,8,4 : 14 ; Si 4+ ionic radius Å)

5 Titanium

6 Zirconium vs Titanium vs Silicon

7 ZirChrom COLUMNS Specification

8 Application note/publications ZirChrom Application Notes - Sorted by Number - Sorted by Column - Sorted by Compound Class Publications Complete Literature Abstracts - Sorted by Subject and Chronologically (Adobe Acrobat Reader required) Complete Literature Abstracts - Sorted Chronologically (Adobe Acrobat Reader required) Publications - Sorted by Subject and Chronologically (Adobe Acrobat Reader required)

9 Product Summary Phase Number Phase Name Mode of Use Application AB01 Rhinophase AB Affinity Phase/Pseudo Antibodies Purification ZRC05 ZirChrom Chiral(R)PG CHIRAL_BrushType, piacceptor ZRC01 ZirChrom Chiral(S)LEU CHIRAL_BrushType, piacceptor ZRC04 ZirChrom Chiral(S)PG CHIRAL_BrushType, piacceptor ZRC02 ZirChrom Chiral(R)NESA CHIRAL_BrushType, pidonor ZRC03 ZirChrom Chiral(S)NESA CHIRAL_BrushType, pidonor ZRC06 ZirChrom CelluloZe CHIRAL_Carbohydrate ZR08 ZirChrom PEZ IEX_CationExchange Mab, Proteins ZR06 ZirChrom SAX IEX_Strong AnionExchange Bio Molecule, Aqueous Vitamins ZR07 ZirChrom SHAX IEX_Strong Hydrophilic AnionExchange Protein_Hydrophilic ZR05 ZirChrom WAX IEX_Weak AnionExchange Bio Molecule, Sugar Analysis ZR04 ZirChrom WCX IEX_Weak CationExchange Proteins TI02 Sachtopore NP Normal Phase and SEC ZR02 ZirChrom PHASE Normal Phase and SEC Steroids, Plant Genins DB01 DiamondBond C18 ReversedPhase T101 Sachtopore RP ReversedPhase TI01 Sachtopore RP ReversedPhase ST01 ZirChrom SELECT ReversedPhase ZR01 ZirChrom CARB ReversedPhase Closely Related Compounds EZ01 ZirChrom EZ ReversedPhase Deactivated, Acidic & Basic MS01 ZirChrom MS ReversedPhase Deactivated/Covalent bond, MS ZR03 ZirChrom PBD (USP L49) ReversedPhase Base & Neutral Compuounds ZR09 ZirChrom PS ReversedPhase High Aqueous Eluents PT01 ProTain ReversedPhase? In-line Protein Removal System Z-APPS ReversedPhase, NP? sub-2μm The MetaloxTM 200-C Syste Column Part m Pre/Post Heat/Cool NT/TT NuTip /TopTip Sample Prep_Zr, Ti Phosphopeptide Enrichment

10 RPC

11 RPC_ZIRCHROM PBD USP L 49 A reversed-phase like material, but with slightly different selectivity. Stable from ph Not as hydrophobic as typical C18-silica phases, so less organic solvent is required. Selectivity is modifiable through the addition of a strong Lewis base to the mobile phase such as fluoride, phosphate or hydroxide. Thermal stability is so high it may be used indefinitely at temperatures up to 150 o C, and is much more stable than new Zorbax TM phases. These higher temperatures result in lower pressure drops, narrower peaks, very high speed analyses and different selectivity.

12 RPC_ZIRCHROM PBD USP L 49

13 RPC_ZIRCHROM PS A reversed-phase like material, useful for separations that require highly aqueous eluents. Stable from ph Not as hydrophobic as typical C18-silica phases, so less organic solvent is required. Selectivity is modifiable through the addition of a strong Lewis base to the mobile phase such as fluoride, phosphate or hydroxide. Thermal stability is so high it may be used indefinitely at temperatures up to 150 o C, and is much more stable than new Zorbax TM phases. These higher temperatures result in lower pressure drops, narrower peaks, very high speed analyses and different selectivity.

14 RPC_ DIAMOND BOND-C18

15

16 RPC_ ZIRCHROM CARB Quality comparable or better than Hypercarb TM at a lower price. Very different selectivity than ODS or polymeric materials; a good alternative when ODS does not work. Better for geometrical isomer separations than ODS or normal phase LC. Superb for separating diastereomers.

17 RPC_ ZIRCHROM EZ/MS Covalent attachment

18 RPC_ ZIRCHROM SELECT

19 Sachtopore-RP, titania-based

20 NPC _ ZIRCHROM-PHASE - SEC Stable from ph 1-14 and at temperatures up to 150 o C. Bare zirconia may be used as an extremely stable SEC phase, which does not produce tailing with amines. A support that may be modified for different selectivity by adding fluoride to the mobile phase. A very stable, regenerable alternative to fragile and expensive hydroxyapatite for protein chromatography.

21 Sachtopore-NP, titania-based

22 IEC

23 IEC_ ZIRCHROM-SAX

24 IEC_ ZIRCHROM-SHAX

25 IEC_ ZIRCHROM-WAX

26 IEC_ ZIRCHROM-WCX

27 IEC_ ZIRCHROM-PEZ

28 CC_ ZIRCHROM-CHIRAL

29 CC_ ZIRCHROM-CHIRAL ZirChrom -Chiral(R)NESA The chiral selector for this column is (R)-N-[1-(1-naphthyl)ethyl]succinamic acid. ZirChrom -Chiral(R)PG The chiral selector for this column is (R)-3,5-dinitrobenzoyl-phenylglycine. ZirChrom -Chiral(S)LEU The chiral selector for this column is (S)-3,5-dinitrobenzoyl-leucine. ZirChrom -Chiral(S)NESA The chiral selector for this column is (S)-N-[1-(1-naphthyl)ethyl]succinamic acid. ZirChrom -Chiral(S)PG The chiral selector for this column is (S)-3,5-dinitrobenzoyl-phenylglycine. ZirChrom -CelluloZe The chiral selector class for this column is polysaccharide.

30 AC_ RHINOPHASE-AB

31 AC_ RHINOPHASE-AB

32 In line protein removal system_ ProTain

33 In line protein removal system_ ProTain Up to 5 mg of bovine serum proteins can be loaded onto a 20 mm x 4.0 mm i.d. cartridge depending on the mobile phase conditions.

34 Sample Prep_ Phosphopeptide enrichment

35 Sample Prep_ Nu Tip/Top Tip Glygen s Lab-in-a-tip SPE pipette tips

36 ZirChrom Rhinophase -AB Kit The ZirChrom Rhinophase -AB Production Kit (part# AB01-HTP) consists of the following: One container containing 30 ml of Rhinophase -AB media (25 um) Sixteen large (60 ml) SPE tubes with frits One 16-port vacuum manifold system One vacuum/pressure pump (110V) Four containers of dry loading buffer (each for 2000 ml solution) Four containers of dry elution buffer (each for 2000 ml solution) Eight empty 2000 ml plastic containers (for loading and elution buffers) The ZirChrom Rhinophase -AB Research Kit (part# AB01-RES) consists of the following: One container containing 3 ml of Rhinophase -AB media (25 um) Three small (6 ml) SPE tubes with frits One container of dry loading buffer (for 2000 ml solution) One containers of dry elution buffer (for 2000 ml solution) Two empty 2000 ml plastic containers (for loading and elution buffers)

37 ZirChrom Rhinophase -AB Kit vs Protein A/G

38 Micro/Capillary column Hotsep micro-hplc columns

39 Guard column