columnsi o n P a c C S 1 6 C a t i o n - E x c h a n g e C o l u m n

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1 columnsi o n P a c C S 6 C a t i o n - E x c h a n g e C o l u m n The IonPac CS6 cation-exchange column is designed for the determination of disparate concentration ratios of sodium and ammonium (up to,:) in diverse sample matrices using an isocratic acid eluent and elevated temperature. Other important applications include the determination of short-chained alkylamines and alkanolamines in the presence of lithium, sodium, ammonium, potassium, magnesium, and calcium in complex sample matrices. The IonPac CS6 column is recommended for most IonPac CS5 applications. The column selectivity is optimized for a C operating temperature for maximum peak efficiencies. Solvent compatibility permits easy column clean-up after the analysis of complex matrices. Superior Chromatographic Performance Universal column for disparate concentration ratios of ammonium and sodium in complex sample matrices Trace level ammonium in high concentrations of sodium; trace level sodium in high concentrations of ammonium or amines Determine amines, including alkylamines and alkanolamines in diverse sample matrices Designed to be used in most IonPac CS5 applications with a larger linear working range Improved low sample ph tolerance High capacity: 8 µeq per column (5 x 5 mm column) Column selectivity optimized for a C operating temperature for maximum peak efficiencies Simplified operation provided by the EG Eluent Generator, which requires only a deionized water source to produce methanesulfonic acid (MSA) eluent Compatible with organic solvents to enhance analyte solubility, to modify column selectivity, or for effective column clean-up Sample matrices include environmental waters and soil extracts; power plant waters treated with ammonium, morpholine, or ethanolamine; chemical additives; chemical process solutions; scrubber solutions; plating baths; and solvents

2 Unique Carboxylate Cation Exchanger The IonPac CS6 column is a unique hydrophilic, high capacity carboxylate functionalized cation exchanger that provides excellent peak shape for alkali and alkaline earth metals and amines. The IonPac CS6 column packing is a 5-µm diameter macroporous particle consisting of ethylvinylbenzene crosslinked with 55% divinylbenzene as shown in Figure. The substrate is functionalized with a hydrophilic carboxylic acid layer that permits the elution of mono- and divalent cations using a dilute hydronium ion eluent, such as MSA or sulfuric acid. The CS6 packing is a new advancement in Dionex resin technology. The use of a smaller resin particle size (5-µm) provides the advantage of improved peak efficiencies, while the new grafting technology allows higher capacity through the incorporation of a much larger number of carboxylic acid cation-exchange sites. Improved Resolution The IonPac CS6 column provides improved resolution among the analyte peaks due to its higher cationexchange capacity. It was designed specifically for high-to-low ratios of sodium and ammonium in diverse sample matrices. Ratios of up to,: can be resolved using a simple isocratic acidic eluent at an elevated temperature ( C) coupled with suppressed conductivity detection. The common inorganic cations and ammonium can be resolved in minutes using mm MSA as shown in Figure. The MSA or sulfuric acid concentration can be optimized for the fast determination of Group I and II cations using 8 mm MSA, also illustrated in Figure..5 µm Macroporous Polymeric Substrate Surface Area = 5 m /g Figure. Structure of an IonPac CS6-5µm packing particle... mm Methanesulfonic acid mm Methanesulfonic acid 5 5 Figure. Isocratic separation of common inorganic cations and ammonium. 6 5 nm 696 Column: IonPac CS6 (5 x 5 mm) Eluent: See chromatogram Flow Rate:. ml/min Temperature: C CSRS -ULTRA -mm, Peaks:. Lithium. mg/l (ppm). Sodium.. Ammonium.5. Potassium. 5. Magnesium.5 6. Calcium. 67

3 High Loading Capacity for Trace Level Ammonium in High Sodium Samples The IonPac CS6 is a high capacity cation-exchange column that replaces the CS5 column for disparate concentration ratios of ammonium and sodium in diverse sample matrices. The CS6 is ideal for the determination of low concentrations of ammonium in environmental waters. It provides improved resolution of sodium from ammonium and alkanolamines, even for samples high in ionic strength. Figure A illustrates the determination of trace level ammonium in the presence of high sodium at ratios up to,: using an isocratic MSA eluent on the CS6 5-mm column. Ratios up to,: can be resolved using a MSA gradient with the CS6 -mm column as illustrated in Figure B..8. A 6 8 B 5 5 Column A: Column B: Eluent A: Eluent B: IonPac CS6 (5 x 5 mm) IonPac CS6 ( x 5 mm) mm Methanesulfonic acid 7 mm Methanesulfonic acid for min to 5 mm at 8 min Flow Rate A:. ml/min Flow Rate B:. ml/min Temperature: C CSRS -ULTRA, Peaks A:. Sodium mg/l (ppm). Ammonium. Peaks B:. Sodium 5. Ammonium.5 CTC- Column placed between the Cell Out and Regen In Ports 79 High Loading Capacity for Trace Level Sodium in High Ammonium Samples The IonPac CS6 column is also ideal for the determination of trace level sodium at ng/l (ppt) concentrations in ammonium and amine-treated cooling waters. The high capacity and unique selectivity of the CS6 column allow the resolution of trace sodium in the presence of high levels of ammonium and alkanolamines. Figures and 5 illustrate the determination of trace concentrations of sodium in the presence of high levels of ammonium or amines. Figure. Determination of trace level ammonium in a high sodium sample Column: IonPac CS6 (5 x 5 mm) Eluent: mm Methanesulfonic acid Temperature: C Flow Rate:. ml/min CSRS -ULTRA -mm, Peaks:. Sodium. mg/l (ppm). Ammonium CTC- Column placed between Cell Out and Regen In Ports 69 Figure. Determination of trace level sodium in a high ammonium sample Column: IonPac CS6 ( x 5 mm) Eluent: 7 mm Methanesulfonic acid for min to 5 mm at 8 min Temperature: C Flow Rate:. ml/min CSRS -ULTRA -mm, Peaks:. Lithium 5 µg/l (ppb). Sodium 6. Ammonium. Ethanolamine 5. Hydrazine 6. Potassium 7. Magnesium 8. Calcium 6 CTC- Column placed between the Cell Out and Regen In Ports 79 Figure 5. Determination of trace level sodium in a high amine sample.

4 Determination of Amines in Complex Matrices The IonPac CS6 column can be used to monitor the amine content in the quality control of chemical additives, process solutions, plating baths, and scrubber solutions. The CS6 column is ideal for the determination of amines in a variety of sample matrices. Figures 6 and 7 illustrate the separation of shortchained hydrophilic methylamines and alkanolamines and Group I and II cations in a single run. For specific amine applications, the eluent conditions can be optimized to resolve the amines of interest and the common inorganic cations Column: IonPac CS6 ( x 5 mm) Eluent: mm Methanesulfonic acid Eluent Source: EG Flow Rate:.5 ml/min Temperature: 6 C CSRS -ULTRA -mm,, 5 ma Peaks:. Lithium.5 mg/l (ppm). Sodium.. Ammonium.. Ethanolamine.5 5. Methylamine.5 6. Potassium.5 7. Dimethylamine Amino--pentanol. 9. Morpholine.. Trimethylamine.5. Magnesium.5. Calcium Figure 6. Determination of methyl amines and the Group I and II cations using the IonPac CS6 column. The CSRS-ULTRA was operated outside the heated column compartment Column: IonPac CS6 ( x 5 mm) Eluent: 6 mm Methanesulfonic acid, gradient from to min to 8 mm Methanesulfonic acid, gradient from to 5 min to 55 mm Methanesulfonic acid Eluent Source: EG Flow Rate:.5 ml/min Temperature: 6 C CSRS -ULTRA -mm, AutoSuppression recycle mode, ma Peaks:. Lithium. mg/l (ppm). Sodium.. Ammonium.5. Ethanolamine.5 5. Diethanolamine. 6. Potassium. 7. Triethanolamine Magnesium.5 9. Calcium. 798 Figure 7. Determination of alkanolamines and the Group I and II cations using the IonPac CS6 column. The CSRS-ULTRA was operated outside the heated column compartment.

5 Long-Term Durability The IonPac CS6 packing ensures long-term column stability and troublefree operation. The column is compatible with acidic eluents and samples. Performance of the high-capacity CS6 column does not deteriorate with the injection of acidic samples up to approximately mn hydronium ion as illustrated in Figure 8. Acid digests or preserved samples can be injected without ph adjustment. Solvent-Compatible Packing The IonPac CS6 column is % solvent-compatible. Adding acetonitrile to the eluent modifies column selectivity and enables the elution of nonpolar analytes or contaminants from the column. Acetonitrile can be used to enhance sample solubility, reduce retention times, and improve the peak shapes of hydrophobic amines. Time and expense can be saved by eliminating time-consuming sample preparation steps. This feature allows complex sample matrices to be analyzed with minimal sample preparation mm H mm H + 5 mm H Column: IonPac CS6 (5 x 5 mm) Eluent: mm Methanesulfonic acid Flow Rate:. ml/min Temperature: C CSRS -ULTRA -mm, Peaks:. Lithium. mg/l (ppm). Sodium.. Ammonium.5. Potassium. 5. Magnesium.5 6. Calcium. Figure 8. The IonPac CS6 packing permits the injection of acidic samples without ph adjustment. 67

6 Ordering Information In the U.S., call or contact the Dionex regional office nearest you. Outside the U.S., order through your local Dionex office or distributor. Refer to the following part numbers. Description Part Number IonPac CS6 Columns IonPac CS6 Analytical Column (5 x 5 mm) IonPac CG6 Guard Column (5 x 5 mm) IonPac CS6 Analytical Column ( x 5 mm) IonPac CG6 Guard Column ( x 5 mm) Trap Columns CTC- Cation Trap Column (9 x mm) for use with the 5-mm columns...9 CTC Cation Trap Column ( x 5 mm) for use with the -mm columns... Cation Concentrator Columns TCC-LP Low Pressure Trace Cation Concentrator ( x 5 mm) for use with the -mm and 5-mm columns...67 Ordering Information For optimum ease of use and economy, the IonPac CS6 column should be operated with the CSRS Cation Self-Regenerating Suppressor. When performing gradient cationexchange applications on the IonPac CS6 column, a Cation Trap Column (CTC-) should be installed between the gradient pump and the injection valve to remove cationic contaminants from the eluent. The EG Eluent Generator automatically produces MSA gradients from water. When using the EG for eluent delivery, a CTC may be required depending on source water purity. When performing concentrator work, the TCC-LP Cation Concentrator column or the CG6 should be used as the concentrator column. S p e c i f i c at i o n s Dimensions: CS6 Analytical Column: 5 x 5 mm and x 5 mm CG6 Guard Column: 5 x 5 mm and x 5 mm Maximum Operating Pressure: psi Mobile Phase Compatibility: Acidic eluents, % HPLC acetonitrile. Alcohols should be avoided. Substrate Characteristics: Bead Diameter: 5 µm Crosslinking (%DVB): 55% Ion-Exchange Group: Grafted carboxylic acid Functional Group Characteristics: Medium hydrophobic Capacity (µeq/column): 8 µeq/column (5 x 5 mm) 7 µeq/column (5 x 5 mm) µeq/column ( x 5 mm) 6 µeq/column ( x 5 mm) Column Construction: PEEK with - threaded ferrulestyle end fittings. All components are non-metallic. IonPac, Cation Self-Regenerating Suppressor, and CSRS are registered trademarks of Dionex Corporation. Printed on recycled and recyclable paper. Passion. Power. Productivity. Dionex Corporation North America Europe Asia Pacific 8 Titan Way P.O. Box 6 U.S. (87) Canada (95) Austria () Belgium () 5 9 Denmark (5) France () 9 Germany (9) Italy (9) Sunnyvale, CA The Netherlands () Switzerland () South America U.K. and Ireland () Brazil (55) 7 5 (8) Australia (6) 9 5 China (85) 8 8 India (9) 8755 Japan (8) Korea (8) Singapore (65) LPN 57- PDF /6 6 Dionex Corporation