LaserBio Labs. Catalog and Price list March Peptide and protein calibration kits Purified MALDI matrices MALDI matrix kits Protein digests

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1 Catalog and Price list March 2003 Peptide and protein calibration kits Purified MALDI matrices MALDI matrix kits Protein digests

2 Matrix Kits Our MALDI matrix kits contain pre-weighed recrystallized matrices and adapted solvent mixtures. They provide standard analytical conditions and eliminate the need for cumbersome daily preparation of fresh matrix. Recrystallized matrix provides higher sensitivity and improved signal-to-noise ratio when compared to non-recrystallized standard commercial product. M001 αchca matrix kit Contains 50 eppendorf tubes of 5 mg recrystallized α-cyano-4-hydroxycinnamic acid (αchca) matrix each, and one 50 ml solvent vial (50% acetonitrile in water / 0.1% Trifluoroacetic acid) for matrix preparation Applications: MALDI-TOF analysis of peptides and proteins, Proteomics M002 SA matrix kit Contains 50 eppendorf tubes of 5 mg recrystallized 3,5 dimethoxy-4-hydroxycinnamic acid (Sinapinic acid - SA) matrix each, and one 50 ml solvent vial (30% acetonitrile in water / 0.1% Trifluoroacetic acid) for matrix preparation Applications: MALDI-TOF analysis of peptides and proteins M003 DHB matrix kit Contains 50 eppendorf tubes of 5 mg recrystallized 2,5 dihydroxybenzoic acid (Gentisic acid - DHB) matrix each, and one 50 ml solvent vial (20% acetonitrile in water / 0.1% Trifluoroacetic acid) for matrix preparation Applications: MALDI-TOF analysis of peptides and proteins, small molecules M004 Peptide/protein 3-matrix kit eppendorf tubes of 5 mg recrystallized α-cyano-4-hydroxycinnamic acid (αchca) each 30 eppendorf tubes of 5 mg recrystallized 3,5 dimethoxy-4-hydroxycinnamic acid (Sinapinic acid - SA) each 30 eppendorf tubes of 5 mg recrystallized 2,5 dihydroxybenzoic acid (Gentisic acid - DHB) matrix each Three solvent vials of 30 ml each (50% acetonitrile in water / 0.1% TFA, 30% acetonitrile in water / 0.1% TFA, 20% acetonitrile in water / 0.1% TFA) for matrix preparation Applications: MALDI-TOF analysis of peptides and proteins, proteomics, small molecules Bulk Matrix M101 Recrystallized αchca matrix tubes of 200 mg each recrystallized α-cyano-4-hydroxycinnamic acid (αchca) matrix M102 Recrystallized SA matrix tubes of 200 mg each recrystallized 3,5 dimethoxy-4-hydroxycinnamic acid (Sinapinic acid - SA) matrix M103 Recrystallized DHB matrix tubes of 200 mg each recrystallized 2,5 dihydroxybenzoic acid (Gentisic acid - DHB) matrix M104 Recrystallized Peptide/protein matrix tubes of 200 mg each recrystallized α-cyano-4-hydroxycinnamic acid (αchca), 3,5 dimethoxy-4-hydroxycinnamic acid (Sinapinic acid SA) and 2,5 dihydroxybenzoic acid (Gentisic acid - DHB) matrix Page 1 info@laserbiolabs.com

3 Peptide Calibration Mixtures C101 Peptide calibration mix 1 (PepMix1) Da tubes of peptide calibration mixture 1. Each tube provides 100 calibration points Contains : Angiotensin II Angiotensin I Neurotensin ACTH [1-17] ACTH [18-39] ( Da) ( Da) ( Da) ( Da) ( Da) C102 Peptide calibration mix 2 (PepMix2) Da tubes of peptide calibration mixture 2. Each tube provides 100 calibration points Contains : Neurotensin ( Da) ACTH [18-39] ( Da) Bovine Insulin chain B ( Da) Bovine Insulin ( Da) C103 Peptide calibration mix 3 (PepMix3) Da tubes of peptide calibration mixture 3. Each tube provides 100 calibration points Bovine Insulin chain B ( Da) Bovine Insulin ( Da) Aprotinin ( Da) Ubiquitin bovine ( Da) C104 Peptide calibration mix 4 (ProteoMix) Da tubes of peptide calibration mixture 4. Each tube provides 100 calibration points. Specially developed for calibration of proteolytic peptide mixtures and proteomics applications Bradykinin [1-5] (572.7 Da) Angiotensin II ( Da) Neurotensin ( Da) ACTH [18-39] ( Da) Bovine Insulin chain B ( Da) Page 2 info@laserbiolabs.com

4 C105 Peptide calibration mix 5 (PepMix5) Da tubes of peptide calibration mixture 5. Each tube provides 100 calibration points Bradykinin [1-5] Bradykinin [1-7] Bradykinin Angiotensin I Neurotensin (572.7 Da) (756.9 Da) ( Da) ( Da) ( Da) C106 Peptide calibration mix 6 (PepMix6) Da tubes of peptide calibration mixture 6. Each tube provides 100 calibration points Bradykinin [1-5] Bradykinin [1-7] Bradykinin [1-8] (572.7 Da) (756.9 Da) (904.0 Da) Protein Calibration Mixtures C107 Protein calibration mix 1 (ProMix1) 5,700-17,000 Da tubes of protein calibration mixture 1. Each tube provides 100 calibration points Bovine Insulin ( Da) Horse heart cytochrome C ( Da) Horse myoglobin ( Da) C108 Protein calibration mix 2 (ProMix2) 8,000-24,000 Da tubes of protein calibration mixture 2. Each tube provides 100 calibration points Horse heart cytochrome C ( Da) Horse myoglobin ( Da) Trypsinogen ( Da) Page 3 info@laserbiolabs.com

5 C109 Protein calibration mix 3 (ProMix3) 23,000-66,000 Da tubes of protein calibration mixture 3. Each tube provides 100 calibration points Trypsinogen ( Da) Yeast enolase ( Da) Bovine serum albumin ( Da) C110 Protein calibration standards 12,000-66,000 Da tubes of individual protein calibrants. Each tube provides 100 calibration points Horse heart cytochrome C Horse myoglobin Trypsinogen Yeast enolase Bovine serum albumin ( Da) ( Da) ( Da) ( Da) ( Da) Calibration Kits Our calibration kits contain calibrated peptide and protein mixtures, designed for analytical reproducibility and consistent signal-to-noise ratio, as well as individual calibrants and diluent. They provide reproducible, standard analytical conditions and eliminate the need for cumbersome preparation of calibrant mixtures. They are prepared and conditioned with the highest purity chemicals, in a form adapted to everyday use in the mass spectrometry laboratory. C001 Peptide calibration Kit I ( Da) Peptide calibration mixtures 1 (PepMix1), 2 (PepMix2) and 3 (PepMix3). 1 tube each. Each tube provides 100 calibration points Four single calibrant peptides: Angiotensin II ( Da), ACTH [18-39] ( Da), Bovine Insulin chain B ( Da), Bovine Insulin ( Da) 1 tube of mixture diluent (0.01% TFA) C002 Peptide calibration Kit II ( Da) Peptide calibration mixtures 1 (PepMix1), 5 (PepMix5) and 6 (PepMix6). 1 tube each. Each tube provides 100 calibration points Four single calibrant peptides: Bradykinin [1-5] (572.7 Da), Angiotensin II ( Da), Neurotensin ( Da), ACTH [18-39] ( Da) 1 tube of mixture diluent (0.01% TFA) C003 Protein calibration Kit (5-100 kda) tube each of protein calibration mixtures 1 (ProMix1), 2 (ProMix2) and 3 (ProMix3). Each tube provides 100 calibration points Four single calibrant proteins: Bovine Insulin ( Da), Horse myoglobin ( Da), Yeast enolase ( Da), Bovine serum albumin ( Da) 1 tube of mixture diluent (0.01% TFA) C004 Peptide / Protein calibration Kit (1-100 kda) tube each of peptide calibration mixtures 1 (PepMix1), 2 (PepMix2) and 3 (PepMix3). Each tube provides 100 calibration points 1 tube each of protein calibration mixtures 2 (ProMix2) and 3 (ProMix3). Each tube provides 100 calibration points Four single calibrant peptides: Angiotensin II ( Da), ACTH [18-39] ( Da), Bovine Insulin chain B ( Da), Bovine Insulin ( Da) Four single calibrant proteins: Horse myoglobin ( Da), Trypsinogen ( Da), Yeast enolase ( Da), Bovine serum albumin ( Da) 1 tube of mixture diluent (0.01% TFA) Page 4 info@laserbiolabs.com

6 Protein trypsin digests Kits Our protein digests kits contain calibrated peptide mixtures, obtained from controlled trypsin digestion of well characterized proteins. They provide quantified, standardized digests for proteomics and LC-MS applications. They eliminate the variability and cumbersome preparation of trypsin digestions. Digests are prepared with pure natural proteins and conditioned, in a form adapted to everyday use in the mass spectrometry laboratory. D101 Myoglobin trypsin digest tubes of digest D101. Each tube contains 50 picomoles of digested protein T2 T18 T41 1 T3 T10 T17 T24 1 T13 T16 T33 1 T1 D102 Enolase trypsin digest tubes of digest D102. Each tube contains 50 picomoles of digested protein T50 T3 T22 T30 T16 T69 1T11 T4 T45 T44 T38 T14 T82 1 T6 T51 T103 1 T141 2 D103 Glutamate dehydrogenase trypsin digest tubes of digest D103. Each tube contains 50 picomoles of digested protein T14 T53 T59 T15 T3 T47 T20 T37 T63 1 T32 T85 1T751 T12 T28 T50 T89 1 T35 T106 1 D104 BSA trypsin digest tubes of digest D103. Each tube contains 50 picomoles of digested protein T56 T31 T18 T67 T6 T49 T76 T54 T59 T47 T37 T137 1 T76 Page 5 info@laserbiolabs.com

7 D105 Glycogen phosphorylase trypsin digest tubes of digest D105. Each tube contains 50 picomoles of digested protein T86 T70 T22 T98 T107 T49 T20 T65 T47 T92 T96 T46 T63 T29 T41 T94 T34 T25 T23 T84 T21 T143 1 T88 D106 Trypsin digest Mix tube each of digests D101 (Myoglobin), D102 (Enolase), D103 (Glutamate dehydrogenase, D104 (BSA) and D105 (Glycogen phosphorylase). Each tube contains 50 picomoles of digested protein Page 6 info@laserbiolabs.com

8 Ordering Information Catalog # Designation Price ( ) M001 αchca matrix kit 100 M002 SA matrix kit 100 M003 DHB matrix kit 100 M004 Peptide / protein 3-matrix kit (αchca, SA, DHB) 180 M101 Purified αchca matrix 130 M102 Purified SA matrix 150 M103 Purified DHB matrix 120 M104 Purified peptide / protein matrix kit (αchca / SA / DHB) 120 C001 Peptide calibration kit I ( Da) 200 C002 Peptide calibration kit II ( Da) 200 C003 Protein calibration kit (5-100 kda) 200 C004 Peptide / Protein calibration kit (1-100 kda) 300 C101 Peptide calibration PepMix 1 ( Da) 150 C102 Peptide calibration PepMix 2 ( Da) 150 C103 Peptide calibration PepMix 3 ( Da) 150 C104 Peptide calibration PepMix 4 (Proteomix) ( Da) 150 C105 Peptide calibration PepMix 5 ( Da) 150 C106 Peptide calibration PepMix 6 ( Da) 150 C107 Protein calibration ProMix 1 (5,700-17,000 Da) 150 C108 Protein calibration ProMix 2 (8,000-24,000 Da) 150 C109 Protein calibration ProMix 3 (23,000-66,000 Da) 150 C110 Protein calibrants (12,000-66,000 Da) 150 D101 Myoglobin trypsin digest 150 D102 Enolase trypsin digest 150 D103 Glutamate dehydrogenase trypsin digest 150 D104 BSA trypsin digest 150 D105 Glycogen phosphorylase trypsin digest 150 D106 Trypsin digests Mix 150 Prices do not include VAT, shipping & handling Service and custom orders For questions about our product line, problems with products or technical advice on protocoles, please contact us at the address below. We are committed to providing adequate analysis solutions to our customers. For custom orders or volume discount, please consult with our representative Route des Crêtes, BP Sophia-Antipolis Cedex, France Tel +33 (0) Fax +33 (0) info@laserbiolabs.com Copyright 2002 SARL. All rights reserved is a registered trademark All products are for research use only, not for drug, household or other use Page 7 info@laserbiolabs.com