M-Beads Magnetic Silica Beads Tools

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1 M-Beads Magnetic Silica Beads Tools

2 MoBiTec GmbH 2010 Page 2 Contents Technical Data... 3 Application... 4 General Information... 4 Bead Usage... 4 Additional Materials Needed... 4 Protocols... 5 Protein Immobilization... 5 Order Information, Shipping and Storage... 6 Contact and Support... 7

3 MoBiTec GmbH 2010 Page 3 Technical Data Product Name M-Beads Magnetic Silica Beads Tools Size 600 nm 1.0 µm 10 mg/ml Concentration beads/ml 7-12 x Supplied product volume 2 ml, 10 ml, 100 ml Material Magnetic silica beads with activated surface. CI 90% nm µm Size Distribution Sedimentation M-Beads Magnetic silica beads Tools, surface activated: PBS (ph 7.4), 0.05% Sodium Azide (NaN Solution additives 3, Toxic!) M-Beads S, unmodified silica beads: water, 0.05% Sodium Azide Storage Store at 4-8 C Material Supplied Vial with M-Beads suspended in PBS buffer containing 0.05% Sodium Azide. Unmodified M-Beads Magnetic silica beads S are stored in water and 0.05% sodium azide.

4 MoBiTec GmbH 2010 Page 4 Application General Information M-Beads Tools are magnetic silica beads with different surfaces to best suit your needs. Surfaces: Surface activated Formula Example Applications Silica Carboxyl SiOH COOH end-users own application (e.g. functionization of the M-Beads) Protein and peptide immobilization Antibody immobilization Aldehyde CHO Protein immobilization Amine NH 2 Protein immobilization Sulfydril SH immobilization via target cysteine groups, coupling to gold surfaces The magnetic properties allow easy and quick rinsing steps during modification and in their application. The beads can be used to immobilize your own protein or molecule to the surface. Or you can use the positive or negative charge to separate molecules by controlling the ph. Bead Usage This product is stable at least 1 year after production date when stored at 4-8 C, unless mentioned otherwise on the label. Store beads in well closed vial and in upright position to prevent drying of the beads since this makes them more difficult to resuspend. Do not freeze the product! Vortex bead suspension well before use. If you expect iron interference in downstream applications, we strongly advise you to rinse the beads before usage. The M-Beads Magnetic silica beads Tools are suspended in PBS buffer or water with 0.05% sodium azide added as a preservative. Before using the beads it's important to rinse them with water or PBS to remove the NaN 3 that could interfere with your test and for safety reasons since NaN 3 is toxic! Additional Materials Needed Depending on the application, some buffers and materials are needed. Magnets for bead separation/collecting. Mixer/vortex to mix samples and resuspend beads.

5 MoBiTec GmbH 2010 Page 5 Protocols Protein Immobilization Bead Surface Carboxyl 1 (COOH) Aldehyde (CHO) Thiol (SH) Chemicals needed EDC/NHS Aldehyde/Amine reaction EDC/NHS followed by PDEA to introduce a reactive disulphide group. Protein binding Amine groups (from lysine and/or as unblocked N- termini) Amine groups Free cysteine Amine 2 (NH 2 ) Gluteraldehyde Amine/aldehyde Treatment No treatment needed. No treatment needed. Reduce disulphides under nondenaturing conditions to generate free cysteine. No treatment needed. Comments Can be used to couple most proteins. Add reducing agent to stabilize amide bond. Useful for proteins containing cysteines. Risk of multiple coupling. Add reducing agent to stabilize amide bond. 1 The first step is to activate the functional groups with N-hydroxysuccinimide in order of creating a highly reactive succinimide ester which reacts with amine groups contained in protein. 2 Gluteraldehyde gives more stable protein binding than the carbodiimide reagents used with carboxylate beads. Abbreviations: EDC, N-ethyl-N'-(dimethylaminopropyl) carbodiimide; NHS, N- hydroxysuccinimide; PDEA, 2-(2-pyridinyldithio) ethane-amine.

6 MoBiTec GmbH 2010 Page 6 Order Information, Shipping and Storage Order# Product Quantity PR-MAG M-Beads Magnetic silica beads S ml PR-MAG M-Beads Magnetic silica beads S ml PR-MAG M-Beads Magnetic silica beads S ml PR-MAG M-Beads Magnetic silica beads S ml PR-MAG M-Beads Magnetic silica beads S ml PR-MAG M-Beads Magnetic silica beads S ml PR-MAG M-Beads Magnetic silica beads S-CHO ml PR-MAG M-Beads Magnetic silica beads S-CHO ml PR-MAG M-Beads Magnetic silica beads S-CHO ml PR-MAG M-Beads Magnetic silica beads S-CHO ml PR-MAG M-Beads Magnetic silica beads S-COOH ml PR-MAG M-Beads Magnetic silica beads S-COOH ml PR-MAG M-Beads Magnetic silica beads S-COOH ml PR-MAG M-Beads Magnetic silica beads S-COOH ml PR-MAG M-Beads Magnetic silica beads S-COOH ml PR-MAG M-Beads Magnetic silica beads S-COOH ml PR-MAG M-Beads Magnetic silica beads S-NH ml PR-MAG M-Beads Magnetic silica beads S-NH ml PR-MAG M-Beads Magnetic silica beads S-NH ml PR-MAG M-Beads Magnetic silica beads S-NH ml PR-MAG M-Beads Magnetic silica beads S-NH ml PR-MAG M-Beads Magnetic silica beads S-NH ml PR-MAG M-Beads Magnetic silica beads S-SH ml PR-MAG M-Beads Magnetic silica beads S-SH ml PR-MAG M-Beads Magnetic silica beads S-SH ml PR-MAG M-Beads Magnetic silica beads S-SH ml PR-MAG M-Beads Magnetic silica beads S-SH ml PR-MAG M-Beads Magnetic silica beads S-SH ml shipped at RT; store at 4-8 C

7 MoBiTec GmbH 2010 Page 7 Disclaimer For R&D use only. Not for drug, household or other uses. Product contains 0.05% Sodium Azide which is toxic. Avoid contact with the suspension buffer. When disposing the suspension buffer, flush with large amounts of water. Contact and Support MoBiTec GmbH Lotzestrasse 22a D Goettingen Germany Customer Service General inquiries & orders Technical Service Product information phone: +49 (0) phone: +49 (0) fax: +49 (0) fax: +49 (0) order@mobitec.com info@mobitec.com MoBiTec in your area: Find your local distributor at

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