ZORBAX StableBond HPLC Columns

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1 ZORBAX HPLC Columns Longest column lifetime and best reproducibility for low ph separations down to ph 1 Patented stable column chemistry allows use at high temperature and low ph without degradation Six different bonded phases provide broad selectivity SB-C18, SB-C8, SB-CN, SB-Phenyl, SB-C3, SB-Aq Agilent ZORBAX columns use patented, unique, monofunctional silanes with bulky diisobutyl (SB-C18) or diisopropyl (SB-C8, SB-C3, SB-Phenyl, SB-CN and SB-Aq) side chain groups that sterically protect the key siloxane bond from hydrolytic attack at low ph. packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. The high purity, low acidity silica provides excellent peak shape with acidic, basic, and neutral compounds so that columns are an excellent choice for low ph method development. ZORBAX columns are compatible with all common mobile phases, including very high aqueous mobile phases. High purity (Type B) silica for good peak shape Use the LC Reference Library to answer your chromatography questions Save time on method development, column selection, and LC instrumentation and chromatography troubleshooting. The LC Reference Library has practical answers to many commonly-asked questions, and is available 24 hours a day! To access this useful information, go to: Sterically Protected Bonded Phase Bulky diisobutyl (C18) or diisopropyl (C8, C3, CN, Aq, Phenyl) side-chain groups are used to stabilize both long and short chain bonded phases. R= Isopropyl, isobutyl (C18) R1= C8, C18, CN, C3, etc. SB-C18 Shows Excellent Stability at Low ph and High Temperature (ph 0.8, 90 C) Relative Retention SB-C18 (Diisobutyl-C18) ZORBAX Rx-C18 (Dimethyl-C18) Competitor A-C18 Competitor B-C18 Competitor C-C18 Competitor D-C18 Competitor E-C18 Column Volumes of Mobile Phase Purge As an indicator of column breakdown, retention time of toluene was measured after purging the column with mobile phase. Only the SB-C18 is unchanged after three working months of use under these very low ph (0.8) and high temperature (90 C) conditions. ZORBAX Rx-C18 also provides a stable matrix, and can be used as an alternative selectivity to SB-C18. SB-C18 P/N ZORBAX Rx-C18 P/N Purge Solvent: 50% Methanol/50% Water with 1.0% TFA Temperature: 90 C Solute: Toluene 16 Order online at:

2 ZORBAX HPLC Columns Shorter Chain ZORBAX SB-CN is also Stable at Low ph (ph 2.0, 50 C) % k Remaining Column Volumes SB-CN Optimizes Retention and Resolution SB-CN Sterically Protected Silane P/N Si-(Me) 2 PrCN Traditional Silane ZORBAX SB-CN, and the other short chain bonded phases, are also exceptionally stable at low ph. Conventional dimethyl CN and similar bonded phases lack this stability. SB-C18 P/N SB-CN P/N Column: ZORBAX SB 4.6 x 75 mm, 3.5 µm Mobile Phase: 30% ACN 70% 25mM NaH 2 PO 4, ph 2.5 Temperature: 35 C Sample: 1. Estriol 2. Daidzen 3. Quercetin 4. Genistein 5. Diethylstilbestrol The SB-CN column is used here to reduce analysis time by 50%. The retention of the most hydrophobic analyte is cut in half. At the same time retention of the more polar, early eluting peaks increases slightly. Order online at: 17

3 ZORBAX HPLC Columns Five Different Bonded Phases Provide Selectivity Options Find replacement LC part numbers on line SB-C18 P/N When you need to order a replacement LC part, there is nothing like a diagram to help you find the part number you need. Agilent s online Illustrated Parts Breakdown has diagrams and part numbers for 1100, 1050 and 1090 LCs. To take the guesswork out of ordering parts: Go to SB-C8 P/N SB-C3 P/N SB-Phenyl P/N Injection Valve Assembly 1. Valve Assembly 2. Isolation Seal 3. Rotor Seal (Vespel or Tefzel) 4. Stator Ring 5. Stator Face 6. Stator Head 7. Stator Screws Injection Valve SB-CN P/N Columns: ZORBAX 4.6 x 150 mm, 5 µm Mobile Phase: 0-100% B in 18.8 min A: 50 mm NaH 2 PO 4, ph 2.5 in 95% H 2 O / 5% ACN B: 50 mm NaH 2 PO 4, ph 2.5 in 47% H 2 O / 53% ACN Temperature: 26 C UV Detection: 254 nm Sample: 1. Procaine 2. Lidocaine 3. d-cinchonine 4. Butacaine 5. Tetracaine SB-C3 is just one of the five different selectivity choices. In this example, optimum resolution is obtained with SB-C3. All are based on the same high purity Rx-SIL. Selectivity changes are therefore dependent only on the bonded phases, making method development more reliable. 18 Order online at:

4 ZORBAX HPLC Columns Components of Green Tea Separated on a Rapid Resolution SB-C8 Column Column: ZORBAX SB-C8 4.6 x 150 mm, 3.5 µm P/N Mobile Phase: 75% 0.1% TFA : 25% MeOH Temperature: 40 C UV Detection: 280 nm Sample: 1. Epigallocatechin 2. Epicatechin 3. Epigallocatechin gallate 4. Catechol 5. Epicatechin gallate Excellent Peak Shape Nutraceuticals, such as the components of green tea, are quickly separated on a SB-C8 Rapid Resolution column. Column: ZORBAX SB-C x 150 mm, 5 µm P/N Mobile Phase: 85% 0.1% TFA 15% ACN Temperature: 35 C UV Detection: 210 nm Sample: 5 µl 1. Aspartic acid 2. Phenylalanine 3. 5-benzyl-3, 6-dioxo-2-piperazineacetic acid 4. asp-phe 5. Aspartame The high purity Rx-SIL used in the manufacturing of all columns provides excellent peak shape of basic and acidic compounds. Stay current with e-notes more information, more easily, more often Opt-in to Agilent s list and receive the most current information that is relevant to you. Once you register and complete an interest profile, we customize your message to include only information that is important and valuable to you based on your individual needs. Visit and sign-up now! Order online at: 19

5 ZORBAX HPLC Columns ZORBAX Columns (80Å) Ordering Information Column Size Particle SB-C18 SB-C8 SB-CN SB-Phenyl Description (mm) Size (µm) USP L1 USP L7 USP L10 SB-C3 USP L11 SB-Aq Standard Columns Semi-Preparative 9.4 x Semi-Preparative 9.4 x Semi-Preparative 9.4 x Semi-Preparative 9.4 x Analytical 4.6 x Analytical 4.6 x Analytical 4.6 x Rapid Resolution (RR) 4.6 x Rapid Resolution 4.6 x Rapid Resolution 4.6 x Rapid Resolution 4.6 x Rapid Resolution HT 4.6 x Rapid Resolution HT, 3/pk 4.6 x Solvent Saver 3.0 x Solvent Saver 3.0 x Solvent Saver Plus 3.0 x Solvent Saver Plus 3.0 x Narrow Bore 2.1 x Narrow Bore 2.1 x Narrow Bore RR* 2.1 x Narrow Bore RR 2.1 x Narrow Bore RR 2.1 x Narrow Bore RR 2.1 x Narrow Bore RRHT** 2.1 x Narrow Bore RRHT, 3/pk 2.1 x MicroBore RR 1.0 x MicroBore RR 1.0 x MicroBore RR 1.0 x MicorBore Guard, 3/pk 1.0 x Guard Cartridge, 2/pk 9.4 x Guard Cartridge, 4/pk 4.6 x Guard Cartridge, 4/pk 2.1 x Guard Hardware Kit 9.4 x Guard Hardware Kit 4.6 x Guard Hardware Kit 2.1 x PrepHT High Throughput Preparative Columns (endfittings required for use) PrepHT Cartridge 21.2 x PrepHT Cartridge 21.2 x PrepHT Cartridge 21.2 x PrepHT Cartridge 21.2 x PrepHT Cartridge 21.2 x PrepHT Guard Cartridges, 2/pk 17 x Guard Hardware PrepHT Endfittings (required for use) (continued on next page) 20 Order online at:

6 ZORBAX HPLC Columns ZORBAX Columns (80Å) Ordering Information (continued) Column Size Particle SB-C18 SB-C8 Description (mm) Size (µm) USP L1 USP L7 Agilent Cartridge Columns (requires hardware kit ) Analytical 4.6 x Analytical 4.6 x Rapid Resolution 4.6 x Guard Cartridge, 10/pk 4.0 x Cartridge Holder High Throughput Cartridge Columns (requires hardware kit ) Rapid Resolution Cartridge 4.6 x Rapid Resolution Cartridge, 3/pk 4.6 x Rapid Resolution Cartridge 4.6 x Rapid Resolution Cartridge, 3/pk 4.6 x Rapid Resolution Cartridge 2.1 x Rapid Resolution Cartridge, 3/pk 2.1 x Rapid Resolution Cartridge 2.1 x Rapid Resolution Cartridge, 3/pk 2.1 x Rapid Resolution HT Cartridge 4.6 x Rapid Resolution HT Cartridge, 3/pk 4.6 x Rapid Resolution HT Cartridge 4.6 x Rapid Resolution HT Cartridge, 3/pk 4.6 x Rapid Resolution HT Cartridge 4.6 x Rapid Resolution HT Cartridge, 3/pk 4.6 x Rapid Resolution HT Cartridge 2.1 x Rapid Resolution HT Cartridge, 3/pk 2.1 x Rapid Resolution HT Cartridge 2.1 x Rapid Resolution HT Cartridge, 3/pk 2.1 x Rapid Resolution HT Cartridge 2.1 x Rapid Resolution HT Cartridge, 3/pk 2.1 x High Throughput Cartridge Column Hardware Kit Capillary Glass-Lined Columns Capillary 0.5 x Capillary 0.5 x Capillary 0.5 x Capillary RR* 0.5 x Capillary RR 0.5 x Capillary 0.3 x Capillary 0.3 x Capillary 0.3 x Capillary RR 0.3 x *RR: Rapid Resolution 3.5 µm columns **RRHT: Rapid Resolution HT 1.8 µm columns Configurations not shown are available upon request. For more information on these and other columns, consult the Agilent Web site at ZORBAX Column Specifications Bonded Phase Pore Size Surface Area Temp. Limits* ph Range* Endcapped Carbon Load ZORBAX SB-C18 80Å 180 m 2 /g 90 C No 10% ZORBAX SB-C3 80Å 180 m 2 /g 80 C No 4.0% ZORBAX SB-C8 80Å 180 m 2 /g 80 C No 5.5% ZORBAX SB-CN 80Å 180 m 2 /g 80 C No 4.0% ZORBAX SB-Phenyl 80Å 180 m 2 /g 80 C No 5.5% ZORBAX SB-Aq 80Å 180 m 2 /g 80 C No proprietary * columns are designed for optimal use at low ph. At ph 6-8, highest column stability for all silica-based columns is obtained by operating at temperatures <40 C and using low buffer concentrations in the range of M. At mid-range ph, Eclipse XDB and Bonus-RP are recommended. Order online at: 21